AlfalfaGEDB | MS.gene70436

M. sativa cultivar XinJiangDaYe / MS.gene70436

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70436.t1	GAU15170.1	54.1	135	59	2	40	171	37	171	1.20E-29	139.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70436.t1	A0A392P375	57.3	124	50	2	51	171	46	169	6.4e-30	140.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene70436	MS.gene71524	PPI
MS.gene70436	MS.gene70424	PPI
MS.gene77472	MS.gene70436	PPI
MS.gene70418	MS.gene70436	PPI
MS.gene70436	MS.gene77464	PPI
MS.gene70436	MS.gene049263	PPI
MS.gene70436	MS.gene044370	PPI
MS.gene70436	MS.gene044326	PPI
MS.gene70436	MS.gene79115	PPI
MS.gene70436	MS.gene70427	PPI
MS.gene70436	MS.gene70476	PPI
MS.gene70436	MS.gene70430	PPI
MS.gene70443	MS.gene70436	PPI
MS.gene70436	MS.gene25704	PPI
MS.gene70436	MS.gene77468	PPI
MS.gene70436	MS.gene71531	PPI
MS.gene70436	MS.gene044323	PPI
MS.gene70436	MS.gene71282	PPI
MS.gene70436	MS.gene71528	PPI
MS.gene70436	MS.gene71284	PPI
MS.gene70436	MS.gene044376	PPI
MS.gene70436	MS.gene045214	PPI
MS.gene70436	MS.gene77466	PPI
MS.gene70436	MS.gene044361	PPI
MS.gene044363	MS.gene70436	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70436.t1	MTR_3g083080	38.863	211	85	7	1	171	1	207	4.01e-33	117
MS.gene70436.t1	MTR_3g083030	34.746	236	84	8	1	171	1	231	1.00e-27	103
MS.gene70436.t1	MTR_3g083050	35.681	213	90	7	1	171	1	208	8.90e-26	98.2
MS.gene70436.t1	MTR_3g083150	34.259	216	90	10	1	169	1	211	1.99e-18	79.0
MS.gene70436.t1	MTR_3g080680	41.406	128	63	6	51	169	74	198	2.55e-17	75.9
MS.gene70436.t1	MTR_3g082420	43.119	109	52	5	56	154	100	208	3.70e-16	73.2
MS.gene70436.t1	MTR_3g082340	43.119	109	52	5	56	154	101	209	7.86e-16	72.4
MS.gene70436.t1	MTR_3g083100	36.306	157	71	6	28	169	31	173	2.27e-15	70.1
MS.gene70436.t1	MTR_8g461300	34.028	144	77	7	41	169	58	198	3.21e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 55 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCTTTCCTCGCAACTTTC+TGG	0.099577	3.1:+66285073	None:intergenic
AACATCATCAGTTATGTAAA+TGG	0.241862	3.1:+66284798	None:intergenic
CTCTTTCCTCGCAACTTTCT+GGG	0.302255	3.1:+66285074	None:intergenic
AAAGGTGATGGCTGTGTATA+AGG	0.310768	3.1:-66284978	MS.gene70436:CDS
TCCTTCACCCAGTCCAGTTC+GGG	0.325347	3.1:+66285019	None:intergenic
TTCCTTCACCCAGTCCAGTT+CGG	0.326573	3.1:+66285018	None:intergenic
CAGTCCTGCTATAACTTTCC+AGG	0.342416	3.1:-66284532	MS.gene70436:CDS
TTCCAGGCCGAAATCTTTGA+TGG	0.364306	3.1:-66284516	MS.gene70436:CDS
AGGAATTCCATCAAAGATTT+CGG	0.371440	3.1:+66284509	None:intergenic
ATCAGATTCGGAACCCGAAC+TGG	0.376408	3.1:-66285032	MS.gene70436:CDS
GTACTTGTCTAACGCAATCT+TGG	0.389784	3.1:+66284753	None:intergenic
TCCTCGCAACTTTCTGGGGA+CGG	0.424630	3.1:+66285079	None:intergenic
TTGTGTTTGATGACCTTGTC+AGG	0.426633	3.1:-66284629	MS.gene70436:CDS
CGCAACTTTCTGGGGACGGT+TGG	0.434829	3.1:+66285083	None:intergenic
AGAAGAAGAGGAAGAAAGAA+AGG	0.437733	3.1:-66284996	MS.gene70436:CDS
ATTCGGAACCCGAACTGGAC+TGG	0.438785	3.1:-66285027	MS.gene70436:CDS
ACATCATCAGTTATGTAAAT+GGG	0.440245	3.1:+66284799	None:intergenic
TAGCTTGAAAGGTAATGTAA+TGG	0.463220	3.1:+66284577	None:intergenic
GCTGAAATCAAAATCAGATT+CGG	0.474119	3.1:-66285044	MS.gene70436:CDS
TTTGATGGAATTCCTTCAGA+TGG	0.478240	3.1:-66284501	MS.gene70436:CDS
AGACAAGTACAATGTTGATC+AGG	0.481976	3.1:-66284740	MS.gene70436:intron
TCAACCCGTCAATGCGTTCA+AGG	0.482744	3.1:-66284606	MS.gene70436:CDS
TATATGTTAGGTAGGCTTCA+TGG	0.483461	3.1:+66284455	None:intergenic
CTGGGTGAAGGAAGAAGAAG+AGG	0.489092	3.1:-66285008	MS.gene70436:CDS
CCTTGGTGTATATGTTAGGT+AGG	0.492491	3.1:+66284447	None:intergenic
AAAACCTTGAACGCATTGAC+GGG	0.497313	3.1:+66284602	None:intergenic
TGTAGCATCTTTAGCTTGAA+AGG	0.500229	3.1:+66284566	None:intergenic
GAAAGTTATAGCAGGACTGC+TGG	0.513254	3.1:+66284536	None:intergenic
TAAAACCTTGAACGCATTGA+CGG	0.514956	3.1:+66284601	None:intergenic
TTGGATGATGCCGTGCAAGC+AGG	0.523717	3.1:+66285102	None:intergenic
AGAGGAAGAAAGAAAGGTGA+TGG	0.530633	3.1:-66284990	MS.gene70436:CDS
TTCGGAACCCGAACTGGACT+GGG	0.533008	3.1:-66285026	MS.gene70436:CDS
TTCCATCAAAGATTTCGGCC+TGG	0.534165	3.1:+66284514	None:intergenic
TCTTTCCTCGCAACTTTCTG+GGG	0.545629	3.1:+66285075	None:intergenic
GAGTCGGATACCTGCTTGCA+CGG	0.558901	3.1:-66285112	MS.gene70436:CDS
GTCTAACGCAATCTTGGAGA+AGG	0.569250	3.1:+66284759	None:intergenic
GACCCTCCACAAGGTTTATG+TGG	0.573237	3.1:-66284832	MS.gene70436:CDS
TCGGCCTGGAAAGTTATAGC+AGG	0.573488	3.1:+66284528	None:intergenic
TCCTTCAGATGGACCGCCTG+TGG	0.573790	3.1:-66284490	MS.gene70436:CDS
ACCCGAACTGGACTGGGTGA+AGG	0.577055	3.1:-66285020	MS.gene70436:CDS
GACGCTATCGACCCTCCACA+AGG	0.594289	3.1:-66284841	MS.gene70436:CDS
GAGCAGAATGTCGATGGAGT+CGG	0.600485	3.1:-66285128	None:intergenic
GTTACAGAGCAGAATGTCGA+TGG	0.600913	3.1:-66285134	None:intergenic
ACCACAGGCGGTCCATCTGA+AGG	0.603009	3.1:+66284489	None:intergenic
GTTATAGCAGGACTGCTGGA+AGG	0.641064	3.1:+66284540	None:intergenic
CGCCACATAAACCTTGTGGA+GGG	0.644987	3.1:+66284830	None:intergenic
CCGCCACATAAACCTTGTGG+AGG	0.646649	3.1:+66284829	None:intergenic
ATTGACGGGTTGACCTGACA+AGG	0.647456	3.1:+66284616	None:intergenic
ATGGCACACGAACTAACCAC+AGG	0.663365	3.1:+66284474	None:intergenic
ATACCGCCACATAAACCTTG+TGG	0.663420	3.1:+66284826	None:intergenic
TGGAGAAGGTAATGAGACGA+GGG	0.666829	3.1:+66284773	None:intergenic
TTGGAGAAGGTAATGAGACG+AGG	0.668902	3.1:+66284772	None:intergenic
CCTCCACAAGGTTTATGTGG+CGG	0.672471	3.1:-66284829	MS.gene70436:CDS
ACCGTCCCCAGAAAGTTGCG+AGG	0.682379	3.1:-66285080	MS.gene70436:CDS
GCACACGAACTAACCACAGG+CGG	0.703587	3.1:+66284477	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTATTCTTCTTGATTATT+AGG	-	chr3.1:66284921-66284940	MS.gene70436:intron	15.0%
!!	ATTATTCTTCTTGATTATTA+GGG	-	chr3.1:66284922-66284941	MS.gene70436:intron	15.0%
!!	AACAAAAGATAGAACATAAA+AGG	+	chr3.1:66284658-66284677	None:intergenic	20.0%
!!	AATATATCAAGTGAAACAAA+GGG	+	chr3.1:66284689-66284708	None:intergenic	20.0%
!!	ATATTATCTTAATCTCATTG+TGG	-	chr3.1:66284704-66284723	MS.gene70436:intron	20.0%
!!	TAATATATCAAGTGAAACAA+AGG	+	chr3.1:66284690-66284709	None:intergenic	20.0%
!!	TATATATACCAATAGTTTAC+TGG	+	chr3.1:66284875-66284894	None:intergenic	20.0%
!	AACATCATCAGTTATGTAAA+TGG	+	chr3.1:66284787-66284806	None:intergenic	25.0%
!	ACATCATCAGTTATGTAAAT+GGG	+	chr3.1:66284786-66284805	None:intergenic	25.0%
	AGGAATTCCATCAAAGATTT+CGG	+	chr3.1:66285076-66285095	None:intergenic	30.0%
	ATAAAAGGAAACGTACAGTT+AGG	+	chr3.1:66284643-66284662	None:intergenic	30.0%
	GCTGAAATCAAAATCAGATT+CGG	-	chr3.1:66284538-66284557	MS.gene70436:CDS	30.0%
	GTTCAAAACCAGTAAACTAT+TGG	-	chr3.1:66284864-66284883	MS.gene70436:intron	30.0%
	TAGCTTGAAAGGTAATGTAA+TGG	+	chr3.1:66285008-66285027	None:intergenic	30.0%
	AGAAGAAGAGGAAGAAAGAA+AGG	-	chr3.1:66284586-66284605	MS.gene70436:CDS	35.0%
	AGACAAGTACAATGTTGATC+AGG	-	chr3.1:66284842-66284861	MS.gene70436:CDS	35.0%
	TAAAACCTTGAACGCATTGA+CGG	+	chr3.1:66284984-66285003	None:intergenic	35.0%
	TTTGATGGAATTCCTTCAGA+TGG	-	chr3.1:66285081-66285100	MS.gene70436:CDS	35.0%
!	AAGTGAAACAAAGGGTACAA+AGG	+	chr3.1:66284681-66284700	None:intergenic	35.0%
!!	TGTAGCATCTTTAGCTTGAA+AGG	+	chr3.1:66285019-66285038	None:intergenic	35.0%
	AGAGGAAGAAAGAAAGGTGA+TGG	-	chr3.1:66284592-66284611	MS.gene70436:CDS	40.0%
	GTACTTGTCTAACGCAATCT+TGG	+	chr3.1:66284832-66284851	None:intergenic	40.0%
!	AAAACCTTGAACGCATTGAC+GGG	+	chr3.1:66284983-66285002	None:intergenic	40.0%
!	AAAGGTGATGGCTGTGTATA+AGG	-	chr3.1:66284604-66284623	MS.gene70436:CDS	40.0%
!	TTGTGTTTGATGACCTTGTC+AGG	-	chr3.1:66284953-66284972	MS.gene70436:intron	40.0%
	ACTCTTTCCTCGCAACTTTC+TGG	+	chr3.1:66284512-66284531	None:intergenic	45.0%
	ATACCGCCACATAAACCTTG+TGG	+	chr3.1:66284759-66284778	None:intergenic	45.0%
	CAGTCCTGCTATAACTTTCC+AGG	-	chr3.1:66285050-66285069	MS.gene70436:CDS	45.0%
	CTCTTTCCTCGCAACTTTCT+GGG	+	chr3.1:66284511-66284530	None:intergenic	45.0%
	GAAAGTTATAGCAGGACTGC+TGG	+	chr3.1:66285049-66285068	None:intergenic	45.0%
	GTCTAACGCAATCTTGGAGA+AGG	+	chr3.1:66284826-66284845	None:intergenic	45.0%
	TCTTTCCTCGCAACTTTCTG+GGG	+	chr3.1:66284510-66284529	None:intergenic	45.0%
	TGGAGAAGGTAATGAGACGA+GGG	+	chr3.1:66284812-66284831	None:intergenic	45.0%
	TTCCAGGCCGAAATCTTTGA+TGG	-	chr3.1:66285066-66285085	MS.gene70436:CDS	45.0%
	TTCCATCAAAGATTTCGGCC+TGG	+	chr3.1:66285071-66285090	None:intergenic	45.0%
	TTGGAGAAGGTAATGAGACG+AGG	+	chr3.1:66284813-66284832	None:intergenic	45.0%
	ATCAGATTCGGAACCCGAAC+TGG	-	chr3.1:66284550-66284569	MS.gene70436:CDS	50.0%
	ATGGCACACGAACTAACCAC+AGG	+	chr3.1:66285111-66285130	None:intergenic	50.0%
	CCTCCACAAGGTTTATGTGG+CGG	-	chr3.1:66284753-66284772	MS.gene70436:CDS	50.0%
	CGCCACATAAACCTTGTGGA+GGG	+	chr3.1:66284755-66284774	None:intergenic	50.0%
	CTGGGTGAAGGAAGAAGAAG+AGG	-	chr3.1:66284574-66284593	MS.gene70436:CDS	50.0%
	GACCCTCCACAAGGTTTATG+TGG	-	chr3.1:66284750-66284769	MS.gene70436:CDS	50.0%
	GTTATAGCAGGACTGCTGGA+AGG	+	chr3.1:66285045-66285064	None:intergenic	50.0%
	TCAACCCGTCAATGCGTTCA+AGG	-	chr3.1:66284976-66284995	MS.gene70436:CDS	50.0%
	TCGGCCTGGAAAGTTATAGC+AGG	+	chr3.1:66285057-66285076	None:intergenic	50.0%
	TTCCTTCACCCAGTCCAGTT+CGG	+	chr3.1:66284567-66284586	None:intergenic	50.0%
!!	ATTGACGGGTTGACCTGACA+AGG	+	chr3.1:66284969-66284988	None:intergenic	50.0%
	ATTCGGAACCCGAACTGGAC+TGG	-	chr3.1:66284555-66284574	MS.gene70436:CDS	55.0%
	CCGCCACATAAACCTTGTGG+AGG	+	chr3.1:66284756-66284775	None:intergenic	55.0%
	GAGTCGGATACCTGCTTGCA+CGG	-	chr3.1:66284470-66284489	MS.gene70436:CDS	55.0%
	GCACACGAACTAACCACAGG+CGG	+	chr3.1:66285108-66285127	None:intergenic	55.0%
	TCCTTCACCCAGTCCAGTTC+GGG	+	chr3.1:66284566-66284585	None:intergenic	55.0%
	TTCGGAACCCGAACTGGACT+GGG	-	chr3.1:66284556-66284575	MS.gene70436:CDS	55.0%
	TTGGATGATGCCGTGCAAGC+AGG	+	chr3.1:66284483-66284502	None:intergenic	55.0%
!	TCCTCGCAACTTTCTGGGGA+CGG	+	chr3.1:66284506-66284525	None:intergenic	55.0%
	ACCACAGGCGGTCCATCTGA+AGG	+	chr3.1:66285096-66285115	None:intergenic	60.0%
	ACCGTCCCCAGAAAGTTGCG+AGG	-	chr3.1:66284502-66284521	MS.gene70436:CDS	60.0%
	GACGCTATCGACCCTCCACA+AGG	-	chr3.1:66284741-66284760	MS.gene70436:CDS	60.0%
	TCCTTCAGATGGACCGCCTG+TGG	-	chr3.1:66285092-66285111	MS.gene70436:CDS	60.0%
!	ACCCGAACTGGACTGGGTGA+AGG	-	chr3.1:66284562-66284581	MS.gene70436:CDS	60.0%
!	CGCAACTTTCTGGGGACGGT+TGG	+	chr3.1:66284502-66284521	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	66284461	66285143	66284461	ID=MS.gene70436
chr3.1	mRNA	66284461	66285143	66284461	ID=MS.gene70436.t1;Parent=MS.gene70436
chr3.1	exon	66284958	66285143	66284958	ID=MS.gene70436.t1.exon1;Parent=MS.gene70436.t1
chr3.1	CDS	66284958	66285143	66284958	ID=cds.MS.gene70436.t1;Parent=MS.gene70436.t1
chr3.1	exon	66284741	66284869	66284741	ID=MS.gene70436.t1.exon2;Parent=MS.gene70436.t1
chr3.1	CDS	66284741	66284869	66284741	ID=cds.MS.gene70436.t1;Parent=MS.gene70436.t1
chr3.1	exon	66284461	66284661	66284461	ID=MS.gene70436.t1.exon3;Parent=MS.gene70436.t1
chr3.1	CDS	66284461	66284661	66284461	ID=cds.MS.gene70436.t1;Parent=MS.gene70436.t1

Gene Sequence

>MS.gene70436

ATGTCGATGGAGTCGGATACCTGCTTGCACGGCATCATCCAACCGTCCCCAGAAAGTTGCGAGGAAAGAGTTAAGTCGCTGAAATCAAAATCAGATTCGGAACCCGAACTGGACTGGGTGAAGGAAGAAGAAGAGGAAGAAAGAAAGGTGATGGCTGTGTATAAGGAGCGCTCTCGTCACCTAACTGTACGTTTCCTTTTATGTTCTATCTTTTGTTCCTTTGTACCCTTTGTTTCACTTGATATATTATCTTAATCTCATTGTGGATGCACAGAGCTTCGACGCTATCGACCCTCCACAAGGTTTATGTGGCGGTATCTTTCCCATTTACATAACTGATGATGTTCGCCCTCGTCTCATTACCTTCTCCAAGATTGCGTTAGACAAGTACAATGTTGATCAGGTTCAAAACCAGTAAACTATTGGTATATATAAATATATGTTTATTTATTGATGAACTAATTATTCTTCTTGATTATTAGGGTGCAAATTTTGTGTTTGATGACCTTGTCAGGTCAACCCGTCAATGCGTTCAAGGTTTTAACCATTACATTACCTTTCAAGCTAAAGATGCTACACTACCTTCCAGCAGTCCTGCTATAACTTTCCAGGCCGAAATCTTTGATGGAATTCCTTCAGATGGACCGCCTGTGGTTAGTTCGTGTGCCATGAAGCCTACCTAA

Protein sequence

>MS.gene70436.t1

MSMESDTCLHGIIQPSPESCEERVKSLKSKSDSEPELDWVKEEEEEERKVMAVYKERSRHLTSFDAIDPPQGLCGGIFPIYITDDVRPRLITFSKIALDKYNVDQGANFVFDDLVRSTRQCVQGFNHYITFQAKDATLPSSSPAITFQAEIFDGIPSDGPPVVSSCAMKPT