Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70441.t1 | GAU15170.1 | 61.3 | 93 | 33 | 3 | 28 | 118 | 80 | 171 | 2.40E-21 | 111.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70441.t1 | A0A392P375 | 62.4 | 93 | 32 | 2 | 28 | 118 | 78 | 169 | 1.7e-21 | 111.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70441 | MS.gene71524 | PPI |
| MS.gene044361 | MS.gene70441 | PPI |
| MS.gene79115 | MS.gene70441 | PPI |
| MS.gene044370 | MS.gene70441 | PPI |
| MS.gene70441 | MS.gene044326 | PPI |
| MS.gene70443 | MS.gene70441 | PPI |
| MS.gene71282 | MS.gene70441 | PPI |
| MS.gene70424 | MS.gene70441 | PPI |
| MS.gene71528 | MS.gene70441 | PPI |
| MS.gene044376 | MS.gene70441 | PPI |
| MS.gene044363 | MS.gene70441 | PPI |
| MS.gene70430 | MS.gene70441 | PPI |
| MS.gene77464 | MS.gene70441 | PPI |
| MS.gene71284 | MS.gene70441 | PPI |
| MS.gene70441 | MS.gene049263 | PPI |
| MS.gene70418 | MS.gene70441 | PPI |
| MS.gene70441 | MS.gene70427 | PPI |
| MS.gene77468 | MS.gene70441 | PPI |
| MS.gene70441 | MS.gene044323 | PPI |
| MS.gene77472 | MS.gene70441 | PPI |
| MS.gene045214 | MS.gene70441 | PPI |
| MS.gene71531 | MS.gene70441 | PPI |
| MS.gene77466 | MS.gene70441 | PPI |
| MS.gene70476 | MS.gene70441 | PPI |
| MS.gene25704 | MS.gene70441 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70441.t1 | MTR_3g083080 | 54.945 | 91 | 38 | 1 | 28 | 118 | 120 | 207 | 1.48e-28 | 103 |
| MS.gene70441.t1 | MTR_3g083030 | 49.451 | 91 | 40 | 3 | 29 | 118 | 146 | 231 | 4.28e-23 | 89.7 |
| MS.gene70441.t1 | MTR_3g083050 | 49.451 | 91 | 40 | 3 | 29 | 118 | 123 | 208 | 8.79e-23 | 88.6 |
| MS.gene70441.t1 | MTR_3g083100 | 43.333 | 90 | 39 | 5 | 37 | 116 | 86 | 173 | 2.52e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTACAATAATGAGAATC+AGG | 0.370663 | 3.1:-66355938 | MS.gene70441:intron |
| ACAGTCCGGCTATAACTTCC+AGG | 0.383566 | 3.1:-66355563 | MS.gene70441:CDS |
| CTTCCAGGCCTATGTATGTC+TGG | 0.438867 | 3.1:-66355548 | MS.gene70441:CDS |
| TTGACCTTGTCAAGTCAACC+TGG | 0.498194 | 3.1:-66355644 | MS.gene70441:CDS |
| GGCAGCATCTTCTGCTTCAA+AGG | 0.508408 | 3.1:+66355596 | None:intergenic |
| TGGAGGAGGTTGCACGGTGA+TGG | 0.509571 | 3.1:-66355528 | MS.gene70441:CDS |
| TGCCTATCCTTCCAACAGTC+CGG | 0.513152 | 3.1:-66355577 | MS.gene70441:CDS |
| ATACTCACCACCACAGCTCC+AGG | 0.524393 | 3.1:+66355626 | None:intergenic |
| GGCCTATGTATGTCTGGAGG+AGG | 0.530208 | 3.1:-66355542 | MS.gene70441:CDS |
| CCTCCAGACATACATAGGCC+TGG | 0.530763 | 3.1:+66355545 | None:intergenic |
| GTCTAACGCAAGGTTGCAGA+GGG | 0.535200 | 3.1:+66355960 | None:intergenic |
| GTCAAGTCAACCTGGAGCTG+TGG | 0.537315 | 3.1:-66355636 | MS.gene70441:CDS |
| GGAAGTTATAGCCGGACTGT+TGG | 0.543443 | 3.1:+66355566 | None:intergenic |
| AGCCGGACTGTTGGAAGGAT+AGG | 0.552433 | 3.1:+66355575 | None:intergenic |
| TGTTTGAAGTTTGAGTGGGC+TGG | 0.565616 | 3.1:+66356025 | None:intergenic |
| TATGTGTTAGGTGAGCTTCA+TGG | 0.566494 | 3.1:+66355482 | None:intergenic |
| GAGGCGAATGTGAGTATCAT+CGG | 0.569023 | 3.1:+66355987 | None:intergenic |
| TATTGTACTTGTCTAACGCA+AGG | 0.601897 | 3.1:+66355950 | None:intergenic |
| AGAATTGTTTGAAGTTTGAG+TGG | 0.609514 | 3.1:+66356020 | None:intergenic |
| ATAGGCCTGGAAGTTATAGC+CGG | 0.610977 | 3.1:+66355558 | None:intergenic |
| AAGTCAACCTGGAGCTGTGG+TGG | 0.611633 | 3.1:-66355633 | MS.gene70441:CDS |
| AGCTCCAGGTTGACTTGACA+AGG | 0.616249 | 3.1:+66355640 | None:intergenic |
| GTTATAGCCGGACTGTTGGA+AGG | 0.617832 | 3.1:+66355570 | None:intergenic |
| TGTCTAACGCAAGGTTGCAG+AGG | 0.623057 | 3.1:+66355959 | None:intergenic |
| CAAGGTTGCAGAGGGGAGTG+AGG | 0.623595 | 3.1:+66355968 | None:intergenic |
| GAATTGTTTGAAGTTTGAGT+GGG | 0.628160 | 3.1:+66356021 | None:intergenic |
| GCACGGTGATGGACCGCATG+TGG | 0.632860 | 3.1:-66355517 | MS.gene70441:CDS |
| AACCTCCTCCAGACATACAT+AGG | 0.641482 | 3.1:+66355540 | None:intergenic |
| TATGTCTGGAGGAGGTTGCA+CGG | 0.656503 | 3.1:-66355534 | MS.gene70441:CDS |
| CCAGGCCTATGTATGTCTGG+AGG | 0.671152 | 3.1:-66355545 | MS.gene70441:CDS |
| GCACACGTCTTGACCACATG+CGG | 0.680788 | 3.1:+66355504 | None:intergenic |
| TCTAACGCAAGGTTGCAGAG+GGG | 0.788709 | 3.1:+66355961 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTATCAATATGATAATA+TGG | - | chr3.1:66355825-66355844 | MS.gene70441:intron | 15.0% |
| ! | TTACAATTCTTGATAGAACT+CGG | - | chr3.1:66355676-66355695 | MS.gene70441:intron | 25.0% |
| !!! | AGAGTTTTTGATTACATGAA+GGG | - | chr3.1:66355737-66355756 | MS.gene70441:intron | 25.0% |
| CAAGTACAATAATGAGAATC+AGG | - | chr3.1:66355625-66355644 | MS.gene70441:CDS | 30.0% | |
| ! | ACTGACTACTCTTCTTATTT+AGG | - | chr3.1:66355878-66355897 | MS.gene70441:intron | 30.0% |
| ! | AGAATTGTTTGAAGTTTGAG+TGG | + | chr3.1:66355546-66355565 | None:intergenic | 30.0% |
| ! | CTGACTACTCTTCTTATTTA+GGG | - | chr3.1:66355879-66355898 | MS.gene70441:intron | 30.0% |
| ! | GAATTGTTTGAAGTTTGAGT+GGG | + | chr3.1:66355545-66355564 | None:intergenic | 30.0% |
| !! | GAGAGTTTTTGATTACATGA+AGG | - | chr3.1:66355736-66355755 | MS.gene70441:intron | 30.0% |
| TATTGTACTTGTCTAACGCA+AGG | + | chr3.1:66355616-66355635 | None:intergenic | 35.0% | |
| AACCTCCTCCAGACATACAT+AGG | + | chr3.1:66356026-66356045 | None:intergenic | 45.0% | |
| ATAGGCCTGGAAGTTATAGC+CGG | + | chr3.1:66356008-66356027 | None:intergenic | 45.0% | |
| GAGGCGAATGTGAGTATCAT+CGG | + | chr3.1:66355579-66355598 | None:intergenic | 45.0% | |
| TTGACCTTGTCAAGTCAACC+TGG | - | chr3.1:66355919-66355938 | MS.gene70441:intron | 45.0% | |
| ! | TGTTTGAAGTTTGAGTGGGC+TGG | + | chr3.1:66355541-66355560 | None:intergenic | 45.0% |
| !!! | AAAAATATAAAAAAGTATAT+TGG | - | chr3.1:66355706-66355725 | MS.gene70441:intron | 5.0% |
| ACAGTCCGGCTATAACTTCC+AGG | - | chr3.1:66356000-66356019 | MS.gene70441:CDS | 50.0% | |
| CTTCCAGGCCTATGTATGTC+TGG | - | chr3.1:66356015-66356034 | MS.gene70441:CDS | 50.0% | |
| GGAAGTTATAGCCGGACTGT+TGG | + | chr3.1:66356000-66356019 | None:intergenic | 50.0% | |
| GGCAGCATCTTCTGCTTCAA+AGG | + | chr3.1:66355970-66355989 | None:intergenic | 50.0% | |
| GTCTAACGCAAGGTTGCAGA+GGG | + | chr3.1:66355606-66355625 | None:intergenic | 50.0% | |
| GTTATAGCCGGACTGTTGGA+AGG | + | chr3.1:66355996-66356015 | None:intergenic | 50.0% | |
| TATGTCTGGAGGAGGTTGCA+CGG | - | chr3.1:66356029-66356048 | MS.gene70441:CDS | 50.0% | |
| TCTAACGCAAGGTTGCAGAG+GGG | + | chr3.1:66355605-66355624 | None:intergenic | 50.0% | |
| TGCCTATCCTTCCAACAGTC+CGG | - | chr3.1:66355986-66356005 | MS.gene70441:CDS | 50.0% | |
| TGTCTAACGCAAGGTTGCAG+AGG | + | chr3.1:66355607-66355626 | None:intergenic | 50.0% | |
| !! | AGCTCCAGGTTGACTTGACA+AGG | + | chr3.1:66355926-66355945 | None:intergenic | 50.0% |
| AAGTCAACCTGGAGCTGTGG+TGG | - | chr3.1:66355930-66355949 | MS.gene70441:intron | 55.0% | |
| AGCCGGACTGTTGGAAGGAT+AGG | + | chr3.1:66355991-66356010 | None:intergenic | 55.0% | |
| ATACTCACCACCACAGCTCC+AGG | + | chr3.1:66355940-66355959 | None:intergenic | 55.0% | |
| CCAGGCCTATGTATGTCTGG+AGG | - | chr3.1:66356018-66356037 | MS.gene70441:CDS | 55.0% | |
| CCTCCAGACATACATAGGCC+TGG | + | chr3.1:66356021-66356040 | None:intergenic | 55.0% | |
| GCACACGTCTTGACCACATG+CGG | + | chr3.1:66356062-66356081 | None:intergenic | 55.0% | |
| GGCCTATGTATGTCTGGAGG+AGG | - | chr3.1:66356021-66356040 | MS.gene70441:CDS | 55.0% | |
| GTCAAGTCAACCTGGAGCTG+TGG | - | chr3.1:66355927-66355946 | MS.gene70441:intron | 55.0% | |
| CAAGGTTGCAGAGGGGAGTG+AGG | + | chr3.1:66355598-66355617 | None:intergenic | 60.0% | |
| TGGAGGAGGTTGCACGGTGA+TGG | - | chr3.1:66356035-66356054 | MS.gene70441:CDS | 60.0% | |
| !! | GCACGGTGATGGACCGCATG+TGG | - | chr3.1:66356046-66356065 | MS.gene70441:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66355488 | 66356097 | 66355488 | ID=MS.gene70441 |
| chr3.1 | mRNA | 66355488 | 66356097 | 66355488 | ID=MS.gene70441.t1;Parent=MS.gene70441 |
| chr3.1 | exon | 66355939 | 66356097 | 66355939 | ID=MS.gene70441.t1.exon1;Parent=MS.gene70441.t1 |
| chr3.1 | CDS | 66355939 | 66356097 | 66355939 | ID=cds.MS.gene70441.t1;Parent=MS.gene70441.t1 |
| chr3.1 | exon | 66355488 | 66355685 | 66355488 | ID=MS.gene70441.t1.exon2;Parent=MS.gene70441.t1 |
| chr3.1 | CDS | 66355488 | 66355685 | 66355488 | ID=cds.MS.gene70441.t1;Parent=MS.gene70441.t1 |
| Gene Sequence |
| Protein sequence |
