Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70445.t1 | PNX74769.1 | 56.2 | 178 | 62 | 1 | 6 | 167 | 10 | 187 | 1.90E-43 | 185.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70445.t1 | A0A2K3L8B1 | 56.2 | 178 | 62 | 1 | 6 | 167 | 10 | 187 | 1.4e-43 | 185.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044363 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene71528 | PPI |
| MS.gene70445 | MS.gene70424 | PPI |
| MS.gene77472 | MS.gene70445 | PPI |
| MS.gene70418 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene77464 | PPI |
| MS.gene70445 | MS.gene044326 | PPI |
| MS.gene70445 | MS.gene70427 | PPI |
| MS.gene70445 | MS.gene70476 | PPI |
| MS.gene044370 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene049263 | PPI |
| MS.gene70445 | MS.gene71524 | PPI |
| MS.gene70443 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene25704 | PPI |
| MS.gene70445 | MS.gene77468 | PPI |
| MS.gene70445 | MS.gene71531 | PPI |
| MS.gene70445 | MS.gene044323 | PPI |
| MS.gene79115 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene71282 | PPI |
| MS.gene70430 | MS.gene70445 | PPI |
| MS.gene70445 | MS.gene71284 | PPI |
| MS.gene70445 | MS.gene044376 | PPI |
| MS.gene70445 | MS.gene045214 | PPI |
| MS.gene70445 | MS.gene77466 | PPI |
| MS.gene70445 | MS.gene044361 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70445.t1 | MTR_3g083080 | 50.867 | 173 | 71 | 1 | 2 | 160 | 23 | 195 | 2.96e-52 | 166 |
| MS.gene70445.t1 | MTR_3g083030 | 51.149 | 174 | 67 | 3 | 2 | 159 | 46 | 217 | 3.54e-50 | 161 |
| MS.gene70445.t1 | MTR_3g083050 | 53.548 | 155 | 56 | 2 | 19 | 159 | 42 | 194 | 7.96e-48 | 155 |
| MS.gene70445.t1 | MTR_3g086030 | 42.000 | 150 | 80 | 5 | 9 | 154 | 5 | 151 | 7.42e-27 | 99.8 |
| MS.gene70445.t1 | MTR_3g082420 | 32.990 | 194 | 81 | 5 | 7 | 155 | 17 | 206 | 7.79e-25 | 96.3 |
| MS.gene70445.t1 | MTR_3g082340 | 36.207 | 174 | 77 | 7 | 11 | 155 | 39 | 207 | 2.03e-22 | 90.1 |
| MS.gene70445.t1 | MTR_3g080680 | 31.461 | 178 | 89 | 7 | 9 | 158 | 14 | 186 | 1.03e-15 | 72.0 |
| MS.gene70445.t1 | MTR_3g083150 | 34.857 | 175 | 84 | 4 | 10 | 154 | 22 | 196 | 8.64e-14 | 67.0 |
| MS.gene70445.t1 | MTR_3g083100 | 40.000 | 115 | 46 | 3 | 76 | 167 | 49 | 163 | 5.62e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTCTCCGGTTCCGATTT+TGG | 0.147266 | 3.1:+66407747 | None:intergenic |
| TCCCCTCTGCAAGCTTGCTT+TGG | 0.301525 | 3.1:-66407348 | MS.gene70445:CDS |
| GATACGCAACGAAGAACTTA+TGG | 0.347208 | 3.1:-66407715 | MS.gene70445:CDS |
| CCACATAAACACGCAATTGC+TGG | 0.351740 | 3.1:+66407419 | None:intergenic |
| CGCAATTGCTGGAGGGGAAA+TGG | 0.355122 | 3.1:+66407430 | None:intergenic |
| TGCGAACACATAAACATATA+AGG | 0.360061 | 3.1:+66407657 | None:intergenic |
| AGATGCTTACAGTTAGATTA+CGG | 0.371835 | 3.1:+66407556 | None:intergenic |
| GGTTGAACTTGAAAAGAGAT+TGG | 0.406773 | 3.1:-66406772 | MS.gene70445:CDS |
| ATCGATTCCCACGGCCTCTC+CGG | 0.413278 | 3.1:+66407735 | None:intergenic |
| ACCGACGATGGAGCCAAAAT+CGG | 0.429034 | 3.1:-66407760 | MS.gene70445:CDS |
| CCAGCAATTGCGTGTTTATG+TGG | 0.442705 | 3.1:-66407419 | MS.gene70445:CDS |
| GATGCTTACAGTTAGATTAC+GGG | 0.444024 | 3.1:+66407557 | None:intergenic |
| AATCGGAACCGGAGAGGCCG+TGG | 0.444560 | 3.1:-66407743 | MS.gene70445:CDS |
| TGTGTTCGCACTTTGTGTAC+AGG | 0.475695 | 3.1:-66407644 | MS.gene70445:CDS |
| TGCAGAGGGGAGTGAGTTCA+AGG | 0.476525 | 3.1:+66407360 | None:intergenic |
| TGGCTCCATCGTCGGTGGTA+AGG | 0.478145 | 3.1:+66407767 | None:intergenic |
| AGGTCATCAGTCATGCGATC+AGG | 0.487173 | 3.1:+66407386 | None:intergenic |
| AGCAGGGCAGTTGGAAGGAT+GGG | 0.498741 | 3.1:+66407100 | None:intergenic |
| GATGGAGCCAAAATCGGAAC+CGG | 0.514435 | 3.1:-66407754 | MS.gene70445:CDS |
| TCGGCCTGAAAAGTTATAGC+AGG | 0.516661 | 3.1:+66407083 | None:intergenic |
| GAAATACAATGCTGAGAACC+AGG | 0.518077 | 3.1:-66407324 | MS.gene70445:CDS |
| CTCCAAAGCAAGCTTGCAGA+GGG | 0.518878 | 3.1:+66407346 | None:intergenic |
| TAGCAGGGCAGTTGGAAGGA+TGG | 0.540470 | 3.1:+66407099 | None:intergenic |
| AATAAGTCTTGAAACCTGCC+TGG | 0.544762 | 3.1:+66407306 | None:intergenic |
| AAAAGTTATAGCAGGGCAGT+TGG | 0.545343 | 3.1:+66407091 | None:intergenic |
| TACAATGCTGAGAACCAGGC+AGG | 0.563638 | 3.1:-66407320 | MS.gene70445:intron |
| GGACCTACTGCAAAAGACTT+CGG | 0.565394 | 3.1:+66407064 | None:intergenic |
| TAAACACGCAATTGCTGGAG+GGG | 0.569083 | 3.1:+66407424 | None:intergenic |
| GGGGAGTGAGTTCAAGGCGA+AGG | 0.569994 | 3.1:+66407366 | None:intergenic |
| GTTATAGCAGGGCAGTTGGA+AGG | 0.582712 | 3.1:+66407095 | None:intergenic |
| GCACTTTGTGTACAGGAACA+AGG | 0.588895 | 3.1:-66407637 | MS.gene70445:CDS |
| GCAATTGCGTGTTTATGTGG+CGG | 0.605533 | 3.1:-66407416 | MS.gene70445:CDS |
| TCGTTGCGTATCGATTCCCA+CGG | 0.608411 | 3.1:+66407726 | None:intergenic |
| CATTAGGTCAGGTAAATCAA+AGG | 0.615978 | 3.1:-66406793 | MS.gene70445:intron |
| TCTCCAAAGCAAGCTTGCAG+AGG | 0.618622 | 3.1:+66407345 | None:intergenic |
| TGAAGAAATTAGTGTCGCCA+TGG | 0.621839 | 3.1:-66407598 | MS.gene70445:CDS |
| AGCTACCTTACCACCGACGA+TGG | 0.624415 | 3.1:-66407772 | MS.gene70445:CDS |
| CATAAACACGCAATTGCTGG+AGG | 0.627436 | 3.1:+66407422 | None:intergenic |
| CGGCCTGAAAAGTTATAGCA+GGG | 0.628675 | 3.1:+66407084 | None:intergenic |
| ATCGGAACCGGAGAGGCCGT+GGG | 0.634575 | 3.1:-66407742 | MS.gene70445:CDS |
| ATAAACACGCAATTGCTGGA+GGG | 0.642196 | 3.1:+66407423 | None:intergenic |
| AGCCAAAATCGGAACCGGAG+AGG | 0.658574 | 3.1:-66407749 | MS.gene70445:CDS |
| TCCAAAGCAAGCTTGCAGAG+GGG | 0.721977 | 3.1:+66407347 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTCTTGTTAATTATTA+GGG | - | chr3.1:66407314-66407333 | MS.gene70445:intron | 10.0% |
| !! | TATTATTCTTGTTAATTATT+AGG | - | chr3.1:66407313-66407332 | MS.gene70445:intron | 10.0% |
| !! | TATTCTTATAGACTTAAAAA+TGG | - | chr3.1:66407577-66407596 | MS.gene70445:CDS | 15.0% |
| !!! | TTTTAAATCGTATTCAATTT+TGG | - | chr3.1:66407616-66407635 | MS.gene70445:CDS | 15.0% |
| !! | TAAAAATGGTAACAGTTTAT+CGG | - | chr3.1:66407591-66407610 | MS.gene70445:CDS | 20.0% |
| !!! | TAATCTCTTAATTTTGATTC+TGG | - | chr3.1:66407056-66407075 | MS.gene70445:intron | 20.0% |
| ! | AATTAAGTGAAACGCAAAAT+TGG | + | chr3.1:66407016-66407035 | None:intergenic | 25.0% |
| !!! | CAATTTTGGTATTTAGAATG+TGG | - | chr3.1:66407630-66407649 | MS.gene70445:CDS | 25.0% |
| AATACACAATATTACCTCCA+TGG | + | chr3.1:66407376-66407395 | None:intergenic | 30.0% | |
| AGATGCTTACAGTTAGATTA+CGG | + | chr3.1:66406970-66406989 | None:intergenic | 30.0% | |
| ATACACAATATTACCTCCAT+GGG | + | chr3.1:66407375-66407394 | None:intergenic | 30.0% | |
| ATACTATTAATGCATGAGCT+AGG | + | chr3.1:66407522-66407541 | None:intergenic | 30.0% | |
| TGCGAACACATAAACATATA+AGG | + | chr3.1:66406869-66406888 | None:intergenic | 30.0% | |
| TTAGAATGTGGAATAAGCAT+GGG | - | chr3.1:66407642-66407661 | MS.gene70445:CDS | 30.0% | |
| ! | CAGGCTTACATGTTTAATTT+AGG | - | chr3.1:66407543-66407562 | MS.gene70445:intron | 30.0% |
| ! | TTTAGAATGTGGAATAAGCA+TGG | - | chr3.1:66407641-66407660 | MS.gene70445:CDS | 30.0% |
| !!! | TCTTAATTTTGATTCTGGAC+AGG | - | chr3.1:66407061-66407080 | MS.gene70445:intron | 30.0% |
| CATTAGGTCAGGTAAATCAA+AGG | - | chr3.1:66407730-66407749 | MS.gene70445:CDS | 35.0% | |
| CTCATGCATTAATAGTATGC+AGG | - | chr3.1:66407524-66407543 | MS.gene70445:intron | 35.0% | |
| GATGCTTACAGTTAGATTAC+GGG | + | chr3.1:66406969-66406988 | None:intergenic | 35.0% | |
| GGTTGAACTTGAAAAGAGAT+TGG | - | chr3.1:66407751-66407770 | MS.gene70445:CDS | 35.0% | |
| TATTAATGCATGAGCTAGGT+AGG | + | chr3.1:66407518-66407537 | None:intergenic | 35.0% | |
| TTGATTTACCTGACCTAATG+AGG | + | chr3.1:66407730-66407749 | None:intergenic | 35.0% | |
| AAAAGTTATAGCAGGGCAGT+TGG | + | chr3.1:66407435-66407454 | None:intergenic | 40.0% | |
| AACATTGTCAAGTCAACCCA+TGG | - | chr3.1:66407356-66407375 | MS.gene70445:CDS | 40.0% | |
| AATAAGTCTTGAAACCTGCC+TGG | + | chr3.1:66407220-66407239 | None:intergenic | 40.0% | |
| ATAAACACGCAATTGCTGGA+GGG | + | chr3.1:66407103-66407122 | None:intergenic | 40.0% | |
| GAAATACAATGCTGAGAACC+AGG | - | chr3.1:66407199-66407218 | MS.gene70445:intron | 40.0% | |
| GATACGCAACGAAGAACTTA+TGG | - | chr3.1:66406808-66406827 | MS.gene70445:intron | 40.0% | |
| TGAAGAAATTAGTGTCGCCA+TGG | - | chr3.1:66406925-66406944 | MS.gene70445:intron | 40.0% | |
| ! | TCTTTTGCAGTAGGTCCAAA+CGG | - | chr3.1:66407465-66407484 | MS.gene70445:intron | 40.0% |
| ATGGCACAAGACATGATCAC+AGG | + | chr3.1:66407491-66407510 | None:intergenic | 45.0% | |
| ATTGTCAAGTCAACCCATGG+AGG | - | chr3.1:66407359-66407378 | MS.gene70445:CDS | 45.0% | |
| CAAAGAGCAGCAACCTCATT+AGG | - | chr3.1:66407714-66407733 | MS.gene70445:CDS | 45.0% | |
| CATAAACACGCAATTGCTGG+AGG | + | chr3.1:66407104-66407123 | None:intergenic | 45.0% | |
| CCACATAAACACGCAATTGC+TGG | + | chr3.1:66407107-66407126 | None:intergenic | 45.0% | |
| CCAGCAATTGCGTGTTTATG+TGG | - | chr3.1:66407104-66407123 | MS.gene70445:CDS | 45.0% | |
| CGGCCTGAAAAGTTATAGCA+GGG | + | chr3.1:66407442-66407461 | None:intergenic | 45.0% | |
| GCAATTGCGTGTTTATGTGG+CGG | - | chr3.1:66407107-66407126 | MS.gene70445:CDS | 45.0% | |
| GCACTTTGTGTACAGGAACA+AGG | - | chr3.1:66406886-66406905 | MS.gene70445:intron | 45.0% | |
| GGACCTACTGCAAAAGACTT+CGG | + | chr3.1:66407462-66407481 | None:intergenic | 45.0% | |
| TAAACACGCAATTGCTGGAG+GGG | + | chr3.1:66407102-66407121 | None:intergenic | 45.0% | |
| TCGGCCTGAAAAGTTATAGC+AGG | + | chr3.1:66407443-66407462 | None:intergenic | 45.0% | |
| TGTGTTCGCACTTTGTGTAC+AGG | - | chr3.1:66406879-66406898 | MS.gene70445:intron | 45.0% | |
| ! | CTGCCCTGCTATAACTTTTC+AGG | - | chr3.1:66407436-66407455 | MS.gene70445:CDS | 45.0% |
| ! | GGCTGCATCTTTTGCTTGAA+AGG | + | chr3.1:66407405-66407424 | None:intergenic | 45.0% |
| ACCGACGATGGAGCCAAAAT+CGG | - | chr3.1:66406763-66406782 | MS.gene70445:CDS | 50.0% | |
| AGACATGATCACAGGCCGTT+TGG | + | chr3.1:66407483-66407502 | None:intergenic | 50.0% | |
| AGCAGCAACCTCATTAGGTC+AGG | - | chr3.1:66407719-66407738 | MS.gene70445:CDS | 50.0% | |
| AGGTCATCAGTCATGCGATC+AGG | + | chr3.1:66407140-66407159 | None:intergenic | 50.0% | |
| CATGAGCTAGGTAGGCTTCA+TGG | + | chr3.1:66407510-66407529 | None:intergenic | 50.0% | |
| CTCCAAAGCAAGCTTGCAGA+GGG | + | chr3.1:66407180-66407199 | None:intergenic | 50.0% | |
| GATGGAGCCAAAATCGGAAC+CGG | - | chr3.1:66406769-66406788 | MS.gene70445:CDS | 50.0% | |
| GTTATAGCAGGGCAGTTGGA+AGG | + | chr3.1:66407431-66407450 | None:intergenic | 50.0% | |
| TACAATGCTGAGAACCAGGC+AGG | - | chr3.1:66407203-66407222 | MS.gene70445:intron | 50.0% | |
| TCCAAAGCAAGCTTGCAGAG+GGG | + | chr3.1:66407179-66407198 | None:intergenic | 50.0% | |
| TCTCCAAAGCAAGCTTGCAG+AGG | + | chr3.1:66407181-66407200 | None:intergenic | 50.0% | |
| ! | AGGCCGAAGTCTTTTGCAGT+AGG | - | chr3.1:66407456-66407475 | MS.gene70445:intron | 50.0% |
| !! | GCGCTTTTTATGCTCAGCCA+TGG | + | chr3.1:66406945-66406964 | None:intergenic | 50.0% |
| !! | TCGTTGCGTATCGATTCCCA+CGG | + | chr3.1:66406800-66406819 | None:intergenic | 50.0% |
| !!! | TCCGATTTTGGCTCCATCGT+CGG | + | chr3.1:66406767-66406786 | None:intergenic | 50.0% |
| AGCAGGGCAGTTGGAAGGAT+GGG | + | chr3.1:66407426-66407445 | None:intergenic | 55.0% | |
| AGCCAAAATCGGAACCGGAG+AGG | - | chr3.1:66406774-66406793 | MS.gene70445:CDS | 55.0% | |
| AGCTACCTTACCACCGACGA+TGG | - | chr3.1:66406751-66406770 | MS.gene70445:CDS | 55.0% | |
| CGCAATTGCTGGAGGGGAAA+TGG | + | chr3.1:66407096-66407115 | None:intergenic | 55.0% | |
| TAGCAGGGCAGTTGGAAGGA+TGG | + | chr3.1:66407427-66407446 | None:intergenic | 55.0% | |
| TGCAGAGGGGAGTGAGTTCA+AGG | + | chr3.1:66407166-66407185 | None:intergenic | 55.0% | |
| ! | TCCCCTCTGCAAGCTTGCTT+TGG | - | chr3.1:66407175-66407194 | MS.gene70445:intron | 55.0% |
| !!! | GATTTTGGCTCCATCGTCGG+TGG | + | chr3.1:66406764-66406783 | None:intergenic | 55.0% |
| GGGGAGTGAGTTCAAGGCGA+AGG | + | chr3.1:66407160-66407179 | None:intergenic | 60.0% | |
| TGGCTCCATCGTCGGTGGTA+AGG | + | chr3.1:66406759-66406778 | None:intergenic | 60.0% | |
| ! | ATCGATTCCCACGGCCTCTC+CGG | + | chr3.1:66406791-66406810 | None:intergenic | 60.0% |
| ! | GGCCTCTCCGGTTCCGATTT+TGG | + | chr3.1:66406779-66406798 | None:intergenic | 60.0% |
| AATCGGAACCGGAGAGGCCG+TGG | - | chr3.1:66406780-66406799 | MS.gene70445:CDS | 65.0% | |
| ATCGGAACCGGAGAGGCCGT+GGG | - | chr3.1:66406781-66406800 | MS.gene70445:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66406746 | 66407799 | 66406746 | ID=MS.gene70445 |
| chr3.1 | mRNA | 66406746 | 66407799 | 66406746 | ID=MS.gene70445.t1;Parent=MS.gene70445 |
| chr3.1 | exon | 66407566 | 66407799 | 66407566 | ID=MS.gene70445.t1.exon1;Parent=MS.gene70445.t1 |
| chr3.1 | CDS | 66407566 | 66407799 | 66407566 | ID=cds.MS.gene70445.t1;Parent=MS.gene70445.t1 |
| chr3.1 | exon | 66407321 | 66407462 | 66407321 | ID=MS.gene70445.t1.exon2;Parent=MS.gene70445.t1 |
| chr3.1 | CDS | 66407321 | 66407462 | 66407321 | ID=cds.MS.gene70445.t1;Parent=MS.gene70445.t1 |
| chr3.1 | exon | 66407068 | 66407164 | 66407068 | ID=MS.gene70445.t1.exon3;Parent=MS.gene70445.t1 |
| chr3.1 | CDS | 66407068 | 66407164 | 66407068 | ID=cds.MS.gene70445.t1;Parent=MS.gene70445.t1 |
| chr3.1 | exon | 66406746 | 66406809 | 66406746 | ID=MS.gene70445.t1.exon4;Parent=MS.gene70445.t1 |
| chr3.1 | CDS | 66406746 | 66406809 | 66406746 | ID=cds.MS.gene70445.t1;Parent=MS.gene70445.t1 |
| Gene Sequence |
| Protein sequence |
