Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70511.t1 | XP_003601436.2 | 66.3 | 193 | 53 | 4 | 16 | 197 | 7 | 198 | 7.20E-58 | 233.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70511.t1 | G7J7C7 | 66.3 | 193 | 53 | 4 | 16 | 197 | 7 | 198 | 5.2e-58 | 233.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70511 | MS.gene70418 | PPI |
| MS.gene70511 | MS.gene77464 | PPI |
| MS.gene70511 | MS.gene044370 | PPI |
| MS.gene70511 | MS.gene044326 | PPI |
| MS.gene70511 | MS.gene79115 | PPI |
| MS.gene70511 | MS.gene70427 | PPI |
| MS.gene70511 | MS.gene70476 | PPI |
| MS.gene70511 | MS.gene049263 | PPI |
| MS.gene70511 | MS.gene70443 | PPI |
| MS.gene70511 | MS.gene25704 | PPI |
| MS.gene70511 | MS.gene77468 | PPI |
| MS.gene70511 | MS.gene71531 | PPI |
| MS.gene044363 | MS.gene70511 | PPI |
| MS.gene70511 | MS.gene044323 | PPI |
| MS.gene70511 | MS.gene71282 | PPI |
| MS.gene70511 | MS.gene71284 | PPI |
| MS.gene70511 | MS.gene044376 | PPI |
| MS.gene70511 | MS.gene70430 | PPI |
| MS.gene70511 | MS.gene045214 | PPI |
| MS.gene70511 | MS.gene77466 | PPI |
| MS.gene70511 | MS.gene044361 | PPI |
| MS.gene70511 | MS.gene71524 | PPI |
| MS.gene70511 | MS.gene71528 | PPI |
| MS.gene70511 | MS.gene70424 | PPI |
| MS.gene77472 | MS.gene70511 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70511.t1 | MTR_3g080680 | 67.179 | 195 | 52 | 5 | 14 | 197 | 5 | 198 | 3.42e-82 | 243 |
| MS.gene70511.t1 | MTR_3g082420 | 40.271 | 221 | 108 | 6 | 1 | 197 | 1 | 221 | 6.70e-39 | 133 |
| MS.gene70511.t1 | MTR_3g082340 | 40.541 | 222 | 107 | 8 | 1 | 197 | 1 | 222 | 8.75e-38 | 130 |
| MS.gene70511.t1 | MTR_3g083080 | 41.709 | 199 | 104 | 6 | 7 | 198 | 13 | 206 | 4.92e-36 | 125 |
| MS.gene70511.t1 | MTR_8g461300 | 45.181 | 166 | 77 | 5 | 44 | 197 | 35 | 198 | 1.88e-35 | 124 |
| MS.gene70511.t1 | MTR_4g055800 | 42.361 | 144 | 66 | 5 | 47 | 184 | 144 | 276 | 5.86e-27 | 104 |
| MS.gene70511.t1 | MTR_3g083050 | 38.967 | 213 | 107 | 9 | 1 | 197 | 1 | 206 | 2.98e-25 | 98.2 |
| MS.gene70511.t1 | MTR_3g083030 | 40.331 | 181 | 95 | 7 | 23 | 197 | 56 | 229 | 1.60e-24 | 96.7 |
| MS.gene70511.t1 | MTR_3g083150 | 36.866 | 217 | 111 | 10 | 1 | 197 | 1 | 211 | 1.08e-19 | 83.6 |
| MS.gene70511.t1 | MTR_3g086030 | 39.583 | 96 | 53 | 2 | 33 | 123 | 14 | 109 | 3.52e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATCTCAGGTGGTTTAA+TGG | 0.243396 | 3.1:-67135094 | None:intergenic |
| GCCTGGAAAGTTGTTATATC+AGG | 0.246103 | 3.1:-67135024 | None:intergenic |
| TTACCACGTTTGCCGTAATA+AGG | 0.262079 | 3.1:-67134487 | None:intergenic |
| AATAGATTCTACCATTAATA+AGG | 0.262811 | 3.1:-67134957 | None:intergenic |
| TGTACCCATGCCCTTATTAA+TGG | 0.282280 | 3.1:+67134946 | MS.gene70511:CDS |
| CCGAGAGAGGGATCGGGATC+GGG | 0.369892 | 3.1:+67134400 | MS.gene70511:CDS |
| CCGCCAAGATCAATGATAAC+TGG | 0.380453 | 3.1:+67134690 | MS.gene70511:CDS |
| GAGGATTTCCAGGGCAGGTT+TGG | 0.402561 | 3.1:-67134350 | None:intergenic |
| CCCTGATATAACAACTTTCC+AGG | 0.412336 | 3.1:+67135023 | MS.gene70511:CDS |
| AGTATAAGAATGAGGCATAT+AGG | 0.413071 | 3.1:+67134515 | MS.gene70511:CDS |
| CCATGCAAATAACAATAATC+AGG | 0.424980 | 3.1:+67134791 | MS.gene70511:CDS |
| GGCTCAAATTCAAATCTATA+CGG | 0.426055 | 3.1:+67134546 | MS.gene70511:CDS |
| GATTCCGAATTTCCTTATTA+CGG | 0.437307 | 3.1:+67134475 | MS.gene70511:CDS |
| GCGCCAGTTATCATTGATCT+TGG | 0.437849 | 3.1:-67134693 | None:intergenic |
| GCCGAGAGAGGGATCGGGAT+CGG | 0.440845 | 3.1:+67134399 | MS.gene70511:CDS |
| TTTGCCGTAATAAGGAAATT+CGG | 0.455567 | 3.1:-67134479 | None:intergenic |
| GTTATCATTGATCTTGGCGG+AGG | 0.467990 | 3.1:-67134687 | None:intergenic |
| ATCACTCTCCAAGCTTGCAT+TGG | 0.473320 | 3.1:+67134761 | MS.gene70511:CDS |
| ATAGATTCTACCATTAATAA+GGG | 0.476198 | 3.1:-67134956 | None:intergenic |
| AAGCGGCCGAGAGAGGGATC+GGG | 0.483001 | 3.1:+67134394 | MS.gene70511:CDS |
| GAGAGTAGGGCGAACATCAT+CGG | 0.484219 | 3.1:-67134736 | None:intergenic |
| CCTGATTATTGTTATTTGCA+TGG | 0.484343 | 3.1:-67134791 | None:intergenic |
| AGGCCATGAAACACAAAAGT+TGG | 0.488464 | 3.1:-67134916 | None:intergenic |
| GAAGCGGCCGAGAGAGGGAT+CGG | 0.489234 | 3.1:+67134393 | MS.gene70511:CDS |
| GTGGCACACTCTCTGATCTC+AGG | 0.491079 | 3.1:-67135105 | None:intergenic |
| TACTTTACAAGATCTTCAAC+GGG | 0.491367 | 3.1:-67134436 | None:intergenic |
| CCCGATCCCGATCCCTCTCT+CGG | 0.493483 | 3.1:-67134400 | None:intergenic |
| TTGGAGAGTGATTCGAGAGT+AGG | 0.496843 | 3.1:-67134750 | None:intergenic |
| TGGAGAGTGATTCGAGAGTA+GGG | 0.500523 | 3.1:-67134749 | None:intergenic |
| GTAATAGTCCAATGCAAGCT+TGG | 0.503651 | 3.1:-67134769 | None:intergenic |
| TTCTACCATTAATAAGGGCA+TGG | 0.508172 | 3.1:-67134951 | None:intergenic |
| GTTGGATACCAAACCTGCCC+TGG | 0.512189 | 3.1:+67134342 | MS.gene70511:CDS |
| CCAGTTATCATTGATCTTGG+CGG | 0.521158 | 3.1:-67134690 | None:intergenic |
| GGATCGAAGCGGCCGAGAGA+GGG | 0.529420 | 3.1:+67134388 | MS.gene70511:CDS |
| GTACTTTACAAGATCTTCAA+CGG | 0.537524 | 3.1:-67134437 | None:intergenic |
| TTATCATTGATCTTGGCGGA+GGG | 0.559160 | 3.1:-67134686 | None:intergenic |
| TTTCCTTATTACGGCAAACG+TGG | 0.561333 | 3.1:+67134484 | MS.gene70511:CDS |
| TTGCAGAATGTCGATGGAGT+TGG | 0.564508 | 3.1:+67134324 | None:intergenic |
| TCATCGAGGATTTCCAGGGC+AGG | 0.571513 | 3.1:-67134355 | None:intergenic |
| ATGGCACACTCATTGACCAC+AGG | 0.572965 | 3.1:-67135075 | None:intergenic |
| CCTGGAAAGTTGTTATATCA+GGG | 0.574585 | 3.1:-67135023 | None:intergenic |
| TTCGTGTTGCAGAATGTCGA+TGG | 0.578989 | 3.1:+67134318 | None:intergenic |
| GCTCTGAGCATACTTCAGCC+TGG | 0.579022 | 3.1:-67135041 | None:intergenic |
| GAACATCATCGGATATGTGA+AGG | 0.588800 | 3.1:-67134725 | None:intergenic |
| AGTTGTTATATCAGGGCTGT+CGG | 0.591200 | 3.1:-67135016 | None:intergenic |
| AACATCATCGGATATGTGAA+GGG | 0.592088 | 3.1:-67134724 | None:intergenic |
| TATCATTGATCTTGGCGGAG+GGG | 0.592257 | 3.1:-67134685 | None:intergenic |
| TCTACCATTAATAAGGGCAT+GGG | 0.593250 | 3.1:-67134950 | None:intergenic |
| GTTATATCAGGGCTGTCGGA+AGG | 0.604717 | 3.1:-67135012 | None:intergenic |
| AGGATCGAAGCGGCCGAGAG+AGG | 0.618028 | 3.1:+67134387 | MS.gene70511:CDS |
| GCACACTCTCTGATCTCAGG+TGG | 0.621362 | 3.1:-67135102 | None:intergenic |
| GGGCATGGGTACACTTGACA+AGG | 0.652935 | 3.1:-67134936 | None:intergenic |
| TGAGGCATATAGGAAGCTTG+AGG | 0.665865 | 3.1:+67134525 | MS.gene70511:CDS |
| ACATCATCGGATATGTGAAG+GGG | 0.669393 | 3.1:-67134723 | None:intergenic |
| AGAGCGAGATAAACAACCTG+TGG | 0.683787 | 3.1:+67135059 | MS.gene70511:CDS |
| TGGAGTGTATTAGATTGTAG+TGG | 0.692541 | 3.1:-67135124 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGATTCTACCATTAATA+AGG | - | chr3.1:67134960-67134979 | None:intergenic | 20.0% |
| !! | AGAAGAACAATTAATTAGTT+TGG | - | chr3.1:67134881-67134900 | None:intergenic | 20.0% |
| !! | ATAGATTCTACCATTAATAA+GGG | - | chr3.1:67134959-67134978 | None:intergenic | 20.0% |
| !! | TGTTCTTCTTCATTATATTA+GGG | + | chr3.1:67134892-67134911 | MS.gene70511:intron | 20.0% |
| !! | TTGTTCTTCTTCATTATATT+AGG | + | chr3.1:67134891-67134910 | MS.gene70511:intron | 20.0% |
| !!! | TAATTTTGAGTATAAGAATG+AGG | + | chr3.1:67134507-67134526 | MS.gene70511:CDS | 20.0% |
| AGTATAAGAATGAGGCATAT+AGG | + | chr3.1:67134515-67134534 | MS.gene70511:CDS | 30.0% | |
| CCATGCAAATAACAATAATC+AGG | + | chr3.1:67134791-67134810 | MS.gene70511:CDS | 30.0% | |
| GATTCCGAATTTCCTTATTA+CGG | + | chr3.1:67134475-67134494 | MS.gene70511:CDS | 30.0% | |
| GGCTCAAATTCAAATCTATA+CGG | + | chr3.1:67134546-67134565 | MS.gene70511:CDS | 30.0% | |
| GTACTTTACAAGATCTTCAA+CGG | - | chr3.1:67134440-67134459 | None:intergenic | 30.0% | |
| TACTTTACAAGATCTTCAAC+GGG | - | chr3.1:67134439-67134458 | None:intergenic | 30.0% | |
| TTTGCCGTAATAAGGAAATT+CGG | - | chr3.1:67134482-67134501 | None:intergenic | 30.0% | |
| !! | CCTGATTATTGTTATTTGCA+TGG | - | chr3.1:67134794-67134813 | None:intergenic | 30.0% |
| AAAAAGAGAAAGGATCGAAG+CGG | + | chr3.1:67134377-67134396 | MS.gene70511:CDS | 35.0% | |
| AACATCATCGGATATGTGAA+GGG | - | chr3.1:67134727-67134746 | None:intergenic | 35.0% | |
| ATAGATAGATGCGTACACTT+AGG | - | chr3.1:67134585-67134604 | None:intergenic | 35.0% | |
| ATCCTCGATGAAAAAGAGAA+AGG | + | chr3.1:67134367-67134386 | MS.gene70511:CDS | 35.0% | |
| CCTGGAAAGTTGTTATATCA+GGG | - | chr3.1:67135026-67135045 | None:intergenic | 35.0% | |
| TTCTACCATTAATAAGGGCA+TGG | - | chr3.1:67134954-67134973 | None:intergenic | 35.0% | |
| ! | TCTACCATTAATAAGGGCAT+GGG | - | chr3.1:67134953-67134972 | None:intergenic | 35.0% |
| ! | TTTTTCATCGAGGATTTCCA+GGG | - | chr3.1:67134362-67134381 | None:intergenic | 35.0% |
| !! | ATCCTTTCTCTTTTTCATCG+AGG | - | chr3.1:67134372-67134391 | None:intergenic | 35.0% |
| ACATCATCGGATATGTGAAG+GGG | - | chr3.1:67134726-67134745 | None:intergenic | 40.0% | |
| AGGCCATGAAACACAAAAGT+TGG | - | chr3.1:67134919-67134938 | None:intergenic | 40.0% | |
| AGTTGTTATATCAGGGCTGT+CGG | - | chr3.1:67135019-67135038 | None:intergenic | 40.0% | |
| CCAGTTATCATTGATCTTGG+CGG | - | chr3.1:67134693-67134712 | None:intergenic | 40.0% | |
| CCCTGATATAACAACTTTCC+AGG | + | chr3.1:67135023-67135042 | MS.gene70511:CDS | 40.0% | |
| GAACATCATCGGATATGTGA+AGG | - | chr3.1:67134728-67134747 | None:intergenic | 40.0% | |
| GCCTGGAAAGTTGTTATATC+AGG | - | chr3.1:67135027-67135046 | None:intergenic | 40.0% | |
| GTAATAGTCCAATGCAAGCT+TGG | - | chr3.1:67134772-67134791 | None:intergenic | 40.0% | |
| TCTGATCTCAGGTGGTTTAA+TGG | - | chr3.1:67135097-67135116 | None:intergenic | 40.0% | |
| TGTACCCATGCCCTTATTAA+TGG | + | chr3.1:67134946-67134965 | MS.gene70511:CDS | 40.0% | |
| TTACCACGTTTGCCGTAATA+AGG | - | chr3.1:67134490-67134509 | None:intergenic | 40.0% | |
| TTATCATTGATCTTGGCGGA+GGG | - | chr3.1:67134689-67134708 | None:intergenic | 40.0% | |
| TTTCCTTATTACGGCAAACG+TGG | + | chr3.1:67134484-67134503 | MS.gene70511:CDS | 40.0% | |
| ! | GGTCCAACTTTTGTGTTTCA+TGG | + | chr3.1:67134913-67134932 | MS.gene70511:CDS | 40.0% |
| !! | AGGATCTTCTTCTGCTTTTG+CGG | - | chr3.1:67134995-67135014 | None:intergenic | 40.0% |
| !! | CTTTTTCATCGAGGATTTCC+AGG | - | chr3.1:67134363-67134382 | None:intergenic | 40.0% |
| !! | TTCTTCTGCTTTTGCGGTAA+AGG | - | chr3.1:67134989-67135008 | None:intergenic | 40.0% |
| AGAGCGAGATAAACAACCTG+TGG | + | chr3.1:67135059-67135078 | MS.gene70511:CDS | 45.0% | |
| ATCACTCTCCAAGCTTGCAT+TGG | + | chr3.1:67134761-67134780 | MS.gene70511:CDS | 45.0% | |
| CCGCCAAGATCAATGATAAC+TGG | + | chr3.1:67134690-67134709 | MS.gene70511:CDS | 45.0% | |
| GCGCCAGTTATCATTGATCT+TGG | - | chr3.1:67134696-67134715 | None:intergenic | 45.0% | |
| GTTATCATTGATCTTGGCGG+AGG | - | chr3.1:67134690-67134709 | None:intergenic | 45.0% | |
| TATCATTGATCTTGGCGGAG+GGG | - | chr3.1:67134688-67134707 | None:intergenic | 45.0% | |
| TGAGGCATATAGGAAGCTTG+AGG | + | chr3.1:67134525-67134544 | MS.gene70511:CDS | 45.0% | |
| TGGAGAGTGATTCGAGAGTA+GGG | - | chr3.1:67134752-67134771 | None:intergenic | 45.0% | |
| TTGGAGAGTGATTCGAGAGT+AGG | - | chr3.1:67134753-67134772 | None:intergenic | 45.0% | |
| ATGGCACACTCATTGACCAC+AGG | - | chr3.1:67135078-67135097 | None:intergenic | 50.0% | |
| GAGAGTAGGGCGAACATCAT+CGG | - | chr3.1:67134739-67134758 | None:intergenic | 50.0% | |
| GTTATATCAGGGCTGTCGGA+AGG | - | chr3.1:67135015-67135034 | None:intergenic | 50.0% | |
| GAGGATTTCCAGGGCAGGTT+TGG | - | chr3.1:67134353-67134372 | None:intergenic | 55.0% | |
| GCACACTCTCTGATCTCAGG+TGG | - | chr3.1:67135105-67135124 | None:intergenic | 55.0% | |
| GGGCATGGGTACACTTGACA+AGG | - | chr3.1:67134939-67134958 | None:intergenic | 55.0% | |
| GTGGCACACTCTCTGATCTC+AGG | - | chr3.1:67135108-67135127 | None:intergenic | 55.0% | |
| GTTGGATACCAAACCTGCCC+TGG | + | chr3.1:67134342-67134361 | MS.gene70511:CDS | 55.0% | |
| TCATCGAGGATTTCCAGGGC+AGG | - | chr3.1:67134358-67134377 | None:intergenic | 55.0% | |
| ! | GCTCTGAGCATACTTCAGCC+TGG | - | chr3.1:67135044-67135063 | None:intergenic | 55.0% |
| AAGCGGCCGAGAGAGGGATC+GGG | + | chr3.1:67134394-67134413 | MS.gene70511:CDS | 65.0% | |
| AGGATCGAAGCGGCCGAGAG+AGG | + | chr3.1:67134387-67134406 | MS.gene70511:CDS | 65.0% | |
| CCCGATCCCGATCCCTCTCT+CGG | - | chr3.1:67134403-67134422 | None:intergenic | 65.0% | |
| CCGAGAGAGGGATCGGGATC+GGG | + | chr3.1:67134400-67134419 | MS.gene70511:CDS | 65.0% | |
| GAAGCGGCCGAGAGAGGGAT+CGG | + | chr3.1:67134393-67134412 | MS.gene70511:CDS | 65.0% | |
| GCCGAGAGAGGGATCGGGAT+CGG | + | chr3.1:67134399-67134418 | MS.gene70511:CDS | 65.0% | |
| GGATCGAAGCGGCCGAGAGA+GGG | + | chr3.1:67134388-67134407 | MS.gene70511:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 67134331 | 67135137 | 67134331 | ID=MS.gene70511 |
| chr3.1 | mRNA | 67134331 | 67135137 | 67134331 | ID=MS.gene70511.t1;Parent=MS.gene70511 |
| chr3.1 | exon | 67134331 | 67134588 | 67134331 | ID=MS.gene70511.t1.exon1;Parent=MS.gene70511.t1 |
| chr3.1 | CDS | 67134331 | 67134588 | 67134331 | ID=cds.MS.gene70511.t1;Parent=MS.gene70511.t1 |
| chr3.1 | exon | 67134669 | 67134812 | 67134669 | ID=MS.gene70511.t1.exon2;Parent=MS.gene70511.t1 |
| chr3.1 | CDS | 67134669 | 67134812 | 67134669 | ID=cds.MS.gene70511.t1;Parent=MS.gene70511.t1 |
| chr3.1 | exon | 67134913 | 67135137 | 67134913 | ID=MS.gene70511.t1.exon3;Parent=MS.gene70511.t1 |
| chr3.1 | CDS | 67134913 | 67135137 | 67134913 | ID=cds.MS.gene70511.t1;Parent=MS.gene70511.t1 |
| Gene Sequence |
| Protein sequence |
