Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70516.t1 | XP_003601436.2 | 73.2 | 198 | 36 | 1 | 1 | 181 | 1 | 198 | 5.60E-73 | 283.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70516.t1 | G7J7C7 | 73.2 | 198 | 36 | 1 | 1 | 181 | 1 | 198 | 4.1e-73 | 283.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70516 | MS.gene045214 | PPI |
| MS.gene77466 | MS.gene70516 | PPI |
| MS.gene044370 | MS.gene70516 | PPI |
| MS.gene70476 | MS.gene70516 | PPI |
| MS.gene25704 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene71524 | PPI |
| MS.gene70516 | MS.gene71528 | PPI |
| MS.gene70443 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene70424 | PPI |
| MS.gene70516 | MS.gene77464 | PPI |
| MS.gene044361 | MS.gene70516 | PPI |
| MS.gene79115 | MS.gene70516 | PPI |
| MS.gene70430 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene044326 | PPI |
| MS.gene70516 | MS.gene70427 | PPI |
| MS.gene044376 | MS.gene70516 | PPI |
| MS.gene044363 | MS.gene70516 | PPI |
| MS.gene71284 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene049263 | PPI |
| MS.gene77472 | MS.gene70516 | PPI |
| MS.gene70418 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene71531 | PPI |
| MS.gene70516 | MS.gene044323 | PPI |
| MS.gene77468 | MS.gene70516 | PPI |
| MS.gene70516 | MS.gene71282 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70516.t1 | MTR_3g080680 | 73.232 | 198 | 36 | 1 | 1 | 181 | 1 | 198 | 1.05e-100 | 289 |
| MS.gene70516.t1 | MTR_8g461300 | 46.429 | 168 | 74 | 5 | 26 | 181 | 35 | 198 | 4.65e-36 | 125 |
| MS.gene70516.t1 | MTR_3g082420 | 44.693 | 179 | 92 | 5 | 8 | 181 | 45 | 221 | 1.47e-34 | 122 |
| MS.gene70516.t1 | MTR_3g082340 | 45.399 | 163 | 83 | 4 | 23 | 181 | 62 | 222 | 3.08e-34 | 121 |
| MS.gene70516.t1 | MTR_3g083080 | 42.077 | 183 | 94 | 6 | 5 | 182 | 31 | 206 | 3.43e-31 | 112 |
| MS.gene70516.t1 | MTR_3g083050 | 41.954 | 174 | 86 | 8 | 14 | 181 | 42 | 206 | 1.17e-25 | 98.6 |
| MS.gene70516.t1 | MTR_3g083030 | 40.541 | 185 | 95 | 8 | 3 | 181 | 54 | 229 | 1.93e-25 | 98.6 |
| MS.gene70516.t1 | MTR_4g055800 | 40.141 | 142 | 68 | 4 | 28 | 165 | 145 | 273 | 6.74e-23 | 93.2 |
| MS.gene70516.t1 | MTR_3g083150 | 36.842 | 171 | 96 | 5 | 17 | 181 | 47 | 211 | 3.43e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACAGAAACAGGTGAAAA+AGG | 0.230480 | 3.1:-67206383 | MS.gene70516:CDS |
| TCTGATCTCAGGTGGTTTAA+TGG | 0.243396 | 3.1:+67205655 | None:intergenic |
| TTACCACGTTTGCCGTAATA+AGG | 0.262079 | 3.1:+67206283 | None:intergenic |
| AATAGATTCTACCATTAATA+AGG | 0.262811 | 3.1:+67205792 | None:intergenic |
| TGTACCCATGCCCTTATTAA+TGG | 0.282280 | 3.1:-67205803 | MS.gene70516:CDS |
| ATTAAAATTAAATGTGATCT+TGG | 0.329794 | 3.1:+67206071 | None:intergenic |
| GGCTCAAATTCAAATCTATA+AGG | 0.386127 | 3.1:-67206224 | MS.gene70516:CDS |
| TGGAGTGTCCTTCGAGAATA+GGG | 0.410247 | 3.1:+67206009 | None:intergenic |
| AGTATAAGAATGAGGCATAT+AGG | 0.413071 | 3.1:-67206255 | MS.gene70516:CDS |
| ACAGAGAATGGAACAGAAAC+AGG | 0.416228 | 3.1:-67206392 | None:intergenic |
| TTGGAGTGTCCTTCGAGAAT+AGG | 0.428317 | 3.1:+67206008 | None:intergenic |
| AAAGGGATCGGGATCGGGAT+TGG | 0.430515 | 3.1:-67206365 | MS.gene70516:CDS |
| GATTCCGAATTTCCTTATTA+CGG | 0.437307 | 3.1:-67206295 | MS.gene70516:CDS |
| TTTGCCGTAATAAGGAAATT+CGG | 0.455567 | 3.1:+67206291 | None:intergenic |
| GCTTGGAAAGTTGTTAAAGC+AGG | 0.466031 | 3.1:+67205725 | None:intergenic |
| ATAGATTCTACCATTAATAA+GGG | 0.476198 | 3.1:+67205793 | None:intergenic |
| GAGAATAGGGCGAACATCAT+CGG | 0.483809 | 3.1:+67206022 | None:intergenic |
| GTACGTCACAAGATCTTCAA+TGG | 0.490169 | 3.1:+67206333 | None:intergenic |
| GTGGCACACTCTCTGATCTC+AGG | 0.491079 | 3.1:+67205644 | None:intergenic |
| TTCTACCATTAATAAGGGCA+TGG | 0.508172 | 3.1:+67205798 | None:intergenic |
| AGGCCATGAAACACAAAAGC+TGG | 0.508489 | 3.1:+67205833 | None:intergenic |
| ATTAAATGTGATCTTGGTGG+AGG | 0.515471 | 3.1:+67206077 | None:intergenic |
| AGGACACTCCAAGCTTGCAT+TGG | 0.517715 | 3.1:-67205997 | MS.gene70516:CDS |
| AAAATTAAATGTGATCTTGG+TGG | 0.523290 | 3.1:+67206074 | None:intergenic |
| GTAATAGTCCAATGCAAGCT+TGG | 0.525102 | 3.1:+67205989 | None:intergenic |
| TACGTCACAAGATCTTCAAT+GGG | 0.528436 | 3.1:+67206334 | None:intergenic |
| TGATCTTGGTGGAGGGGCGA+TGG | 0.538731 | 3.1:+67206085 | None:intergenic |
| ATGGCACACTCTTTGACCAC+AGG | 0.540193 | 3.1:+67205674 | None:intergenic |
| CTTGGAAAGTTGTTAAAGCA+GGG | 0.540365 | 3.1:+67205726 | None:intergenic |
| TTTCCTTATTACGGCAAACG+TGG | 0.561333 | 3.1:-67206286 | MS.gene70516:CDS |
| GAACATCATCGGATATGTGA+AGG | 0.588800 | 3.1:+67206033 | None:intergenic |
| GAACACAGCATACTTCAGCT+TGG | 0.589049 | 3.1:+67205708 | None:intergenic |
| AACATCATCGGATATGTGAA+GGG | 0.592088 | 3.1:+67206034 | None:intergenic |
| TCTACCATTAATAAGGGCAT+GGG | 0.593250 | 3.1:+67205799 | None:intergenic |
| TAAATGTGATCTTGGTGGAG+GGG | 0.597056 | 3.1:+67206079 | None:intergenic |
| AGTTGTTAAAGCAGGGGTGT+CGG | 0.599691 | 3.1:+67205733 | None:intergenic |
| TTAAATGTGATCTTGGTGGA+GGG | 0.609839 | 3.1:+67206078 | None:intergenic |
| GCACACTCTCTGATCTCAGG+TGG | 0.621362 | 3.1:+67205647 | None:intergenic |
| TTGGAAAGTTGTTAAAGCAG+GGG | 0.623852 | 3.1:+67205727 | None:intergenic |
| GATGTTCGCCCTATTCTCGA+AGG | 0.637823 | 3.1:-67206017 | MS.gene70516:CDS |
| GGGCATGGGTACACTTGACA+AGG | 0.652935 | 3.1:+67205813 | None:intergenic |
| ACATCATCGGATATGTGAAG+GGG | 0.660369 | 3.1:+67206035 | None:intergenic |
| TGAGGCATATAGGAAGCTTG+AGG | 0.665865 | 3.1:-67206245 | MS.gene70516:CDS |
| TGTTCGAGACAAACAACCTG+TGG | 0.674826 | 3.1:-67205690 | MS.gene70516:CDS |
| TGGAGTGTATTAGATTGTAG+TGG | 0.692541 | 3.1:+67205625 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTTCATTATATTATATTA+GGG | - | chr3.1:67206162-67206181 | MS.gene70516:intron | 10.0% |
| !! | ATTAAAATTAAATGTGATCT+TGG | + | chr3.1:67205951-67205970 | None:intergenic | 15.0% |
| !! | CTTCTTCATTATATTATATT+AGG | - | chr3.1:67206161-67206180 | MS.gene70516:intron | 15.0% |
| !!! | AAGATCACATTTAATTTTAA+TGG | - | chr3.1:67205950-67205969 | MS.gene70516:intron | 15.0% |
| !!! | AGATCACATTTAATTTTAAT+GGG | - | chr3.1:67205951-67205970 | MS.gene70516:intron | 15.0% |
| !! | AATAGATTCTACCATTAATA+AGG | + | chr3.1:67206230-67206249 | None:intergenic | 20.0% |
| !! | AGAAGAACAATTAATTAGTT+TGG | + | chr3.1:67206146-67206165 | None:intergenic | 20.0% |
| !! | ATAGATTCTACCATTAATAA+GGG | + | chr3.1:67206229-67206248 | None:intergenic | 20.0% |
| !!! | TAATTTTGAGTATAAGAATG+AGG | - | chr3.1:67205756-67205775 | MS.gene70516:CDS | 20.0% |
| ! | AAAATTAAATGTGATCTTGG+TGG | + | chr3.1:67205948-67205967 | None:intergenic | 25.0% |
| !!! | CCTGATTATTGTTTTTTTCA+CGG | + | chr3.1:67206055-67206074 | None:intergenic | 25.0% |
| !!! | TCACATTTAATTTTAATGGG+CGG | - | chr3.1:67205954-67205973 | MS.gene70516:intron | 25.0% |
| !!! | TCATTTTGATTCATTCATTC+TGG | - | chr3.1:67205882-67205901 | MS.gene70516:intron | 25.0% |
| AGTATAAGAATGAGGCATAT+AGG | - | chr3.1:67205764-67205783 | MS.gene70516:CDS | 30.0% | |
| CCGTGAAAAAAACAATAATC+AGG | - | chr3.1:67206052-67206071 | MS.gene70516:CDS | 30.0% | |
| GATTCCGAATTTCCTTATTA+CGG | - | chr3.1:67205724-67205743 | MS.gene70516:CDS | 30.0% | |
| GGCTCAAATTCAAATCTATA+AGG | - | chr3.1:67205795-67205814 | MS.gene70516:CDS | 30.0% | |
| TTTGCCGTAATAAGGAAATT+CGG | + | chr3.1:67205731-67205750 | None:intergenic | 30.0% | |
| AACATCATCGGATATGTGAA+GGG | + | chr3.1:67205988-67206007 | None:intergenic | 35.0% | |
| ATAGATAGATGCGTACACTT+AGG | + | chr3.1:67205834-67205853 | None:intergenic | 35.0% | |
| ATTAAATGTGATCTTGGTGG+AGG | + | chr3.1:67205945-67205964 | None:intergenic | 35.0% | |
| CTTGGAAAGTTGTTAAAGCA+GGG | + | chr3.1:67206296-67206315 | None:intergenic | 35.0% | |
| GAACAGAAACAGGTGAAAAA+GGG | - | chr3.1:67205637-67205656 | MS.gene70516:CDS | 35.0% | |
| TACGTCACAAGATCTTCAAT+GGG | + | chr3.1:67205688-67205707 | None:intergenic | 35.0% | |
| TTAAATGTGATCTTGGTGGA+GGG | + | chr3.1:67205944-67205963 | None:intergenic | 35.0% | |
| TTCTACCATTAATAAGGGCA+TGG | + | chr3.1:67206224-67206243 | None:intergenic | 35.0% | |
| TTGGAAAGTTGTTAAAGCAG+GGG | + | chr3.1:67206295-67206314 | None:intergenic | 35.0% | |
| ! | AAGATCTTCTTCAGCTTTTG+CGG | + | chr3.1:67206265-67206284 | None:intergenic | 35.0% |
| ! | TCTACCATTAATAAGGGCAT+GGG | + | chr3.1:67206223-67206242 | None:intergenic | 35.0% |
| AAACAGGTGAAAAAGGGATC+GGG | - | chr3.1:67205643-67205662 | MS.gene70516:CDS | 40.0% | |
| ACATCATCGGATATGTGAAG+GGG | + | chr3.1:67205987-67206006 | None:intergenic | 40.0% | |
| GAAACAGGTGAAAAAGGGAT+CGG | - | chr3.1:67205642-67205661 | MS.gene70516:CDS | 40.0% | |
| GAACATCATCGGATATGTGA+AGG | + | chr3.1:67205989-67206008 | None:intergenic | 40.0% | |
| GCTTGGAAAGTTGTTAAAGC+AGG | + | chr3.1:67206297-67206316 | None:intergenic | 40.0% | |
| GGAACAGAAACAGGTGAAAA+AGG | - | chr3.1:67205636-67205655 | MS.gene70516:CDS | 40.0% | |
| GTAATAGTCCAATGCAAGCT+TGG | + | chr3.1:67206033-67206052 | None:intergenic | 40.0% | |
| GTACGTCACAAGATCTTCAA+TGG | + | chr3.1:67205689-67205708 | None:intergenic | 40.0% | |
| TAAATGTGATCTTGGTGGAG+GGG | + | chr3.1:67205943-67205962 | None:intergenic | 40.0% | |
| TCTGATCTCAGGTGGTTTAA+TGG | + | chr3.1:67206367-67206386 | None:intergenic | 40.0% | |
| TGTACCCATGCCCTTATTAA+TGG | - | chr3.1:67206216-67206235 | MS.gene70516:CDS | 40.0% | |
| TTACCACGTTTGCCGTAATA+AGG | + | chr3.1:67205739-67205758 | None:intergenic | 40.0% | |
| TTTCCTTATTACGGCAAACG+TGG | - | chr3.1:67205733-67205752 | MS.gene70516:CDS | 40.0% | |
| ! | TTCTTCAGCTTTTGCGGTAA+AGG | + | chr3.1:67206259-67206278 | None:intergenic | 40.0% |
| AGGCCATGAAACACAAAAGC+TGG | + | chr3.1:67206189-67206208 | None:intergenic | 45.0% | |
| GAACACAGCATACTTCAGCT+TGG | + | chr3.1:67206314-67206333 | None:intergenic | 45.0% | |
| GAGAATAGGGCGAACATCAT+CGG | + | chr3.1:67206000-67206019 | None:intergenic | 45.0% | |
| TGAGGCATATAGGAAGCTTG+AGG | - | chr3.1:67205774-67205793 | MS.gene70516:CDS | 45.0% | |
| TGTTCGAGACAAACAACCTG+TGG | - | chr3.1:67206329-67206348 | MS.gene70516:CDS | 45.0% | |
| ! | AGTTGTTAAAGCAGGGGTGT+CGG | + | chr3.1:67206289-67206308 | None:intergenic | 45.0% |
| ! | GGTCCAGCTTTTGTGTTTCA+TGG | - | chr3.1:67206183-67206202 | MS.gene70516:intron | 45.0% |
| ! | TGGAGTGTCCTTCGAGAATA+GGG | + | chr3.1:67206013-67206032 | None:intergenic | 45.0% |
| ! | TTGGAGTGTCCTTCGAGAAT+AGG | + | chr3.1:67206014-67206033 | None:intergenic | 45.0% |
| AGGACACTCCAAGCTTGCAT+TGG | - | chr3.1:67206022-67206041 | MS.gene70516:CDS | 50.0% | |
| ATGGCACACTCTTTGACCAC+AGG | + | chr3.1:67206348-67206367 | None:intergenic | 50.0% | |
| GATGTTCGCCCTATTCTCGA+AGG | - | chr3.1:67206002-67206021 | MS.gene70516:CDS | 50.0% | |
| GGTGAAAAAGGGATCGGGAT+CGG | - | chr3.1:67205648-67205667 | MS.gene70516:CDS | 50.0% | |
| GTGAAAAAGGGATCGGGATC+GGG | - | chr3.1:67205649-67205668 | MS.gene70516:CDS | 50.0% | |
| TGGACAACACAACACAACGC+AGG | - | chr3.1:67205902-67205921 | MS.gene70516:intron | 50.0% | |
| AAAGGGATCGGGATCGGGAT+TGG | - | chr3.1:67205654-67205673 | MS.gene70516:CDS | 55.0% | |
| GCACACTCTCTGATCTCAGG+TGG | + | chr3.1:67206375-67206394 | None:intergenic | 55.0% | |
| GGGCATGGGTACACTTGACA+AGG | + | chr3.1:67206209-67206228 | None:intergenic | 55.0% | |
| GTGGCACACTCTCTGATCTC+AGG | + | chr3.1:67206378-67206397 | None:intergenic | 55.0% | |
| TGATCTTGGTGGAGGGGCGA+TGG | + | chr3.1:67205937-67205956 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 67205634 | 67206407 | 67205634 | ID=MS.gene70516 |
| chr3.1 | mRNA | 67205634 | 67206407 | 67205634 | ID=MS.gene70516.t1;Parent=MS.gene70516 |
| chr3.1 | exon | 67206204 | 67206407 | 67206204 | ID=MS.gene70516.t1.exon1;Parent=MS.gene70516.t1 |
| chr3.1 | CDS | 67206204 | 67206407 | 67206204 | ID=cds.MS.gene70516.t1;Parent=MS.gene70516.t1 |
| chr3.1 | exon | 67205968 | 67206117 | 67205968 | ID=MS.gene70516.t1.exon2;Parent=MS.gene70516.t1 |
| chr3.1 | CDS | 67205968 | 67206117 | 67205968 | ID=cds.MS.gene70516.t1;Parent=MS.gene70516.t1 |
| chr3.1 | exon | 67205634 | 67205858 | 67205634 | ID=MS.gene70516.t1.exon3;Parent=MS.gene70516.t1 |
| chr3.1 | CDS | 67205634 | 67205858 | 67205634 | ID=cds.MS.gene70516.t1;Parent=MS.gene70516.t1 |
| Gene Sequence |
| Protein sequence |
