Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71286.t1 | PNX74769.1 | 57 | 172 | 58 | 1 | 4 | 159 | 16 | 187 | 1.20E-42 | 183 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71286.t1 | A0A2K3L8B1 | 57.0 | 172 | 58 | 1 | 4 | 159 | 16 | 187 | 8.6e-43 | 183.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71286 | MS.gene044326 | PPI |
| MS.gene77468 | MS.gene71286 | PPI |
| MS.gene71286 | MS.gene70427 | PPI |
| MS.gene71284 | MS.gene71286 | PPI |
| MS.gene77472 | MS.gene71286 | PPI |
| MS.gene71531 | MS.gene71286 | PPI |
| MS.gene71286 | MS.gene049263 | PPI |
| MS.gene77466 | MS.gene71286 | PPI |
| MS.gene70476 | MS.gene71286 | PPI |
| MS.gene25704 | MS.gene71286 | PPI |
| MS.gene71286 | MS.gene044323 | PPI |
| MS.gene045214 | MS.gene71286 | PPI |
| MS.gene044361 | MS.gene71286 | PPI |
| MS.gene79115 | MS.gene71286 | PPI |
| MS.gene044370 | MS.gene71286 | PPI |
| MS.gene70443 | MS.gene71286 | PPI |
| MS.gene71282 | MS.gene71286 | PPI |
| MS.gene70424 | MS.gene71286 | PPI |
| MS.gene71528 | MS.gene71286 | PPI |
| MS.gene044376 | MS.gene71286 | PPI |
| MS.gene044363 | MS.gene71286 | PPI |
| MS.gene71286 | MS.gene71524 | PPI |
| MS.gene70430 | MS.gene71286 | PPI |
| MS.gene77464 | MS.gene71286 | PPI |
| MS.gene70418 | MS.gene71286 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71286.t1 | MTR_3g083080 | 52.410 | 166 | 65 | 1 | 1 | 152 | 30 | 195 | 8.60e-52 | 164 |
| MS.gene71286.t1 | MTR_3g083030 | 51.829 | 164 | 63 | 2 | 2 | 151 | 56 | 217 | 5.48e-49 | 158 |
| MS.gene71286.t1 | MTR_3g083050 | 53.548 | 155 | 56 | 2 | 11 | 151 | 42 | 194 | 6.29e-48 | 154 |
| MS.gene71286.t1 | MTR_3g086030 | 42.000 | 150 | 80 | 5 | 1 | 146 | 5 | 151 | 4.86e-27 | 99.8 |
| MS.gene71286.t1 | MTR_3g082420 | 35.632 | 174 | 80 | 6 | 1 | 147 | 38 | 206 | 1.19e-24 | 95.5 |
| MS.gene71286.t1 | MTR_3g082340 | 36.207 | 174 | 77 | 7 | 3 | 147 | 39 | 207 | 1.46e-22 | 90.1 |
| MS.gene71286.t1 | MTR_3g080680 | 32.484 | 157 | 79 | 5 | 22 | 156 | 41 | 192 | 1.08e-15 | 71.6 |
| MS.gene71286.t1 | MTR_3g083150 | 34.857 | 175 | 84 | 4 | 2 | 146 | 22 | 196 | 8.54e-14 | 66.6 |
| MS.gene71286.t1 | MTR_3g083100 | 40.000 | 115 | 46 | 3 | 68 | 159 | 49 | 163 | 4.53e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTCTCCGGTTCCGATTT+TGG | 0.147266 | 3.4:-78134882 | None:intergenic |
| TCCCCTCTGCAAGCTTGCTT+TGG | 0.301525 | 3.4:+78135281 | MS.gene71286:CDS |
| GATACGCAACGAAGAACTTA+TGG | 0.347208 | 3.4:+78134914 | MS.gene71286:CDS |
| CCACATAAACACGCAATTGC+TGG | 0.351740 | 3.4:-78135210 | None:intergenic |
| CGCAATTGCTGGAGGGGAAA+TGG | 0.355122 | 3.4:-78135199 | None:intergenic |
| TGCGAACACATAAACATATA+AGG | 0.360061 | 3.4:-78134972 | None:intergenic |
| AGATGCTTACAGTTAGATTA+CGG | 0.371835 | 3.4:-78135073 | None:intergenic |
| GGTTGAACTTGAAAAGAGAT+TGG | 0.406773 | 3.4:+78135857 | MS.gene71286:CDS |
| ATCGATTCCCACGGCCTCTC+CGG | 0.413278 | 3.4:-78134894 | None:intergenic |
| ACCGACGATGGAGCCAAAAT+CGG | 0.429034 | 3.4:+78134869 | None:intergenic |
| CCAGCAATTGCGTGTTTATG+TGG | 0.442705 | 3.4:+78135210 | MS.gene71286:CDS |
| GATGCTTACAGTTAGATTAC+GGG | 0.444024 | 3.4:-78135072 | None:intergenic |
| AATCGGAACCGGAGAGGCCG+TGG | 0.444560 | 3.4:+78134886 | MS.gene71286:CDS |
| TGTGTTCGCACTTTGTGTAC+AGG | 0.475695 | 3.4:+78134985 | MS.gene71286:CDS |
| TGCAGAGGGGAGTGAGTTCA+AGG | 0.476525 | 3.4:-78135269 | None:intergenic |
| AGGTCATCAGTCATGCGATC+AGG | 0.487173 | 3.4:-78135243 | None:intergenic |
| AGCAGGGCAGTTGGAAGGAT+GGG | 0.498741 | 3.4:-78135529 | None:intergenic |
| GATGGAGCCAAAATCGGAAC+CGG | 0.514435 | 3.4:+78134875 | None:intergenic |
| TCGGCCTGAAAAGTTATAGC+AGG | 0.516661 | 3.4:-78135546 | None:intergenic |
| GAAATACAATGCTGAGAACC+AGG | 0.518077 | 3.4:+78135305 | MS.gene71286:CDS |
| CTCCAAAGCAAGCTTGCAGA+GGG | 0.518878 | 3.4:-78135283 | None:intergenic |
| TAGCAGGGCAGTTGGAAGGA+TGG | 0.540470 | 3.4:-78135530 | None:intergenic |
| AATAAGTCTTGAAACCTGCC+TGG | 0.544762 | 3.4:-78135323 | None:intergenic |
| AAAAGTTATAGCAGGGCAGT+TGG | 0.545343 | 3.4:-78135538 | None:intergenic |
| TACAATGCTGAGAACCAGGC+AGG | 0.563638 | 3.4:+78135309 | MS.gene71286:CDS |
| GGACCTACTGCAAAAGACTT+CGG | 0.565394 | 3.4:-78135565 | None:intergenic |
| TAAACACGCAATTGCTGGAG+GGG | 0.569083 | 3.4:-78135205 | None:intergenic |
| GGGGAGTGAGTTCAAGGCGA+AGG | 0.569994 | 3.4:-78135263 | None:intergenic |
| GTTATAGCAGGGCAGTTGGA+AGG | 0.582712 | 3.4:-78135534 | None:intergenic |
| GCACTTTGTGTACAGGAACA+AGG | 0.588895 | 3.4:+78134992 | MS.gene71286:CDS |
| GCAATTGCGTGTTTATGTGG+CGG | 0.605533 | 3.4:+78135213 | MS.gene71286:CDS |
| TCGTTGCGTATCGATTCCCA+CGG | 0.608411 | 3.4:-78134903 | None:intergenic |
| CATTAGGTCAGGTAAATCAA+AGG | 0.615978 | 3.4:+78135836 | MS.gene71286:intron |
| TCTCCAAAGCAAGCTTGCAG+AGG | 0.618622 | 3.4:-78135284 | None:intergenic |
| TGAAGAAATTAGTGTCGCCA+TGG | 0.621839 | 3.4:+78135031 | MS.gene71286:CDS |
| CATAAACACGCAATTGCTGG+AGG | 0.627436 | 3.4:-78135207 | None:intergenic |
| CGGCCTGAAAAGTTATAGCA+GGG | 0.628675 | 3.4:-78135545 | None:intergenic |
| ATCGGAACCGGAGAGGCCGT+GGG | 0.634575 | 3.4:+78134887 | MS.gene71286:CDS |
| ATAAACACGCAATTGCTGGA+GGG | 0.642196 | 3.4:-78135206 | None:intergenic |
| AGCCAAAATCGGAACCGGAG+AGG | 0.658574 | 3.4:+78134880 | MS.gene71286:CDS |
| TCCAAAGCAAGCTTGCAGAG+GGG | 0.721977 | 3.4:-78135282 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTCTTGTTAATTATTA+GGG | + | chr3.4:78135420-78135439 | MS.gene71286:intron | 10.0% |
| !! | TATTATTCTTGTTAATTATT+AGG | + | chr3.4:78135419-78135438 | MS.gene71286:intron | 10.0% |
| !! | TATTCTTATAGACTTAAAAA+TGG | + | chr3.4:78135683-78135702 | MS.gene71286:intron | 15.0% |
| !!! | TTTTAAATCGTATTCAATTT+TGG | + | chr3.4:78135722-78135741 | MS.gene71286:intron | 15.0% |
| !! | TAAAAATGGTAACAGTTTAT+CGG | + | chr3.4:78135697-78135716 | MS.gene71286:intron | 20.0% |
| !!! | TAATCTCTTAATTTTGATTC+TGG | + | chr3.4:78135162-78135181 | MS.gene71286:intron | 20.0% |
| ! | AATTAAGTGAAACGCAAAAT+TGG | - | chr3.4:78135122-78135141 | None:intergenic | 25.0% |
| !!! | CAATTTTGGTATTTAGAATG+TGG | + | chr3.4:78135736-78135755 | MS.gene71286:intron | 25.0% |
| AATACACAATATTACCTCCA+TGG | - | chr3.4:78135482-78135501 | None:intergenic | 30.0% | |
| AGATGCTTACAGTTAGATTA+CGG | - | chr3.4:78135076-78135095 | None:intergenic | 30.0% | |
| ATACACAATATTACCTCCAT+GGG | - | chr3.4:78135481-78135500 | None:intergenic | 30.0% | |
| ATACTATTAATGCATGAGCT+AGG | - | chr3.4:78135628-78135647 | None:intergenic | 30.0% | |
| TGCGAACACATAAACATATA+AGG | - | chr3.4:78134975-78134994 | None:intergenic | 30.0% | |
| TTAGAATGTGGAATAAGCAT+GGG | + | chr3.4:78135748-78135767 | MS.gene71286:intron | 30.0% | |
| ! | CAGGCTTACATGTTTAATTT+AGG | + | chr3.4:78135649-78135668 | MS.gene71286:intron | 30.0% |
| ! | TTTAGAATGTGGAATAAGCA+TGG | + | chr3.4:78135747-78135766 | MS.gene71286:intron | 30.0% |
| !!! | TCTTAATTTTGATTCTGGAC+AGG | + | chr3.4:78135167-78135186 | MS.gene71286:intron | 30.0% |
| CATTAGGTCAGGTAAATCAA+AGG | + | chr3.4:78135836-78135855 | MS.gene71286:intron | 35.0% | |
| CTCATGCATTAATAGTATGC+AGG | + | chr3.4:78135630-78135649 | MS.gene71286:intron | 35.0% | |
| GATGCTTACAGTTAGATTAC+GGG | - | chr3.4:78135075-78135094 | None:intergenic | 35.0% | |
| GGTTGAACTTGAAAAGAGAT+TGG | + | chr3.4:78135857-78135876 | MS.gene71286:CDS | 35.0% | |
| TATTAATGCATGAGCTAGGT+AGG | - | chr3.4:78135624-78135643 | None:intergenic | 35.0% | |
| TTGATTTACCTGACCTAATG+AGG | - | chr3.4:78135836-78135855 | None:intergenic | 35.0% | |
| AAAAGTTATAGCAGGGCAGT+TGG | - | chr3.4:78135541-78135560 | None:intergenic | 40.0% | |
| AACATTGTCAAGTCAACCCA+TGG | + | chr3.4:78135462-78135481 | MS.gene71286:intron | 40.0% | |
| AATAAGTCTTGAAACCTGCC+TGG | - | chr3.4:78135326-78135345 | None:intergenic | 40.0% | |
| ATAAACACGCAATTGCTGGA+GGG | - | chr3.4:78135209-78135228 | None:intergenic | 40.0% | |
| GAAATACAATGCTGAGAACC+AGG | + | chr3.4:78135305-78135324 | MS.gene71286:CDS | 40.0% | |
| GATACGCAACGAAGAACTTA+TGG | + | chr3.4:78134914-78134933 | MS.gene71286:CDS | 40.0% | |
| TGAAGAAATTAGTGTCGCCA+TGG | + | chr3.4:78135031-78135050 | MS.gene71286:CDS | 40.0% | |
| ! | TCTTTTGCAGTAGGTCCAAA+CGG | + | chr3.4:78135571-78135590 | MS.gene71286:intron | 40.0% |
| ATGGCACAAGACATGATCAC+AGG | - | chr3.4:78135597-78135616 | None:intergenic | 45.0% | |
| ATTGTCAAGTCAACCCATGG+AGG | + | chr3.4:78135465-78135484 | MS.gene71286:intron | 45.0% | |
| CAAAGAGCAGCAACCTCATT+AGG | + | chr3.4:78135820-78135839 | MS.gene71286:intron | 45.0% | |
| CATAAACACGCAATTGCTGG+AGG | - | chr3.4:78135210-78135229 | None:intergenic | 45.0% | |
| CCACATAAACACGCAATTGC+TGG | - | chr3.4:78135213-78135232 | None:intergenic | 45.0% | |
| CCAGCAATTGCGTGTTTATG+TGG | + | chr3.4:78135210-78135229 | MS.gene71286:CDS | 45.0% | |
| CGGCCTGAAAAGTTATAGCA+GGG | - | chr3.4:78135548-78135567 | None:intergenic | 45.0% | |
| GCAATTGCGTGTTTATGTGG+CGG | + | chr3.4:78135213-78135232 | MS.gene71286:CDS | 45.0% | |
| GCACTTTGTGTACAGGAACA+AGG | + | chr3.4:78134992-78135011 | MS.gene71286:CDS | 45.0% | |
| GGACCTACTGCAAAAGACTT+CGG | - | chr3.4:78135568-78135587 | None:intergenic | 45.0% | |
| TAAACACGCAATTGCTGGAG+GGG | - | chr3.4:78135208-78135227 | None:intergenic | 45.0% | |
| TCGGCCTGAAAAGTTATAGC+AGG | - | chr3.4:78135549-78135568 | None:intergenic | 45.0% | |
| TGTGTTCGCACTTTGTGTAC+AGG | + | chr3.4:78134985-78135004 | MS.gene71286:CDS | 45.0% | |
| ! | CTGCCCTGCTATAACTTTTC+AGG | + | chr3.4:78135542-78135561 | MS.gene71286:CDS | 45.0% |
| ! | GGCTGCATCTTTTGCTTGAA+AGG | - | chr3.4:78135511-78135530 | None:intergenic | 45.0% |
| AGACATGATCACAGGCCGTT+TGG | - | chr3.4:78135589-78135608 | None:intergenic | 50.0% | |
| AGCAGCAACCTCATTAGGTC+AGG | + | chr3.4:78135825-78135844 | MS.gene71286:intron | 50.0% | |
| AGGTCATCAGTCATGCGATC+AGG | - | chr3.4:78135246-78135265 | None:intergenic | 50.0% | |
| CATGAGCTAGGTAGGCTTCA+TGG | - | chr3.4:78135616-78135635 | None:intergenic | 50.0% | |
| CTCCAAAGCAAGCTTGCAGA+GGG | - | chr3.4:78135286-78135305 | None:intergenic | 50.0% | |
| GTTATAGCAGGGCAGTTGGA+AGG | - | chr3.4:78135537-78135556 | None:intergenic | 50.0% | |
| TACAATGCTGAGAACCAGGC+AGG | + | chr3.4:78135309-78135328 | MS.gene71286:CDS | 50.0% | |
| TCCAAAGCAAGCTTGCAGAG+GGG | - | chr3.4:78135285-78135304 | None:intergenic | 50.0% | |
| TCTCCAAAGCAAGCTTGCAG+AGG | - | chr3.4:78135287-78135306 | None:intergenic | 50.0% | |
| ! | AGGCCGAAGTCTTTTGCAGT+AGG | + | chr3.4:78135562-78135581 | MS.gene71286:CDS | 50.0% |
| !! | GCGCTTTTTATGCTCAGCCA+TGG | - | chr3.4:78135051-78135070 | None:intergenic | 50.0% |
| !! | TCGTTGCGTATCGATTCCCA+CGG | - | chr3.4:78134906-78134925 | None:intergenic | 50.0% |
| AGCAGGGCAGTTGGAAGGAT+GGG | - | chr3.4:78135532-78135551 | None:intergenic | 55.0% | |
| AGCCAAAATCGGAACCGGAG+AGG | + | chr3.4:78134880-78134899 | MS.gene71286:CDS | 55.0% | |
| CGCAATTGCTGGAGGGGAAA+TGG | - | chr3.4:78135202-78135221 | None:intergenic | 55.0% | |
| TAGCAGGGCAGTTGGAAGGA+TGG | - | chr3.4:78135533-78135552 | None:intergenic | 55.0% | |
| TGCAGAGGGGAGTGAGTTCA+AGG | - | chr3.4:78135272-78135291 | None:intergenic | 55.0% | |
| ! | TCCCCTCTGCAAGCTTGCTT+TGG | + | chr3.4:78135281-78135300 | MS.gene71286:CDS | 55.0% |
| GGGGAGTGAGTTCAAGGCGA+AGG | - | chr3.4:78135266-78135285 | None:intergenic | 60.0% | |
| ! | ATCGATTCCCACGGCCTCTC+CGG | - | chr3.4:78134897-78134916 | None:intergenic | 60.0% |
| ! | GGCCTCTCCGGTTCCGATTT+TGG | - | chr3.4:78134885-78134904 | None:intergenic | 60.0% |
| AATCGGAACCGGAGAGGCCG+TGG | + | chr3.4:78134886-78134905 | MS.gene71286:CDS | 65.0% | |
| ATCGGAACCGGAGAGGCCGT+GGG | + | chr3.4:78134887-78134906 | MS.gene71286:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 78134876 | 78135905 | 78134876 | ID=MS.gene71286 |
| chr3.4 | mRNA | 78134876 | 78135905 | 78134876 | ID=MS.gene71286.t1;Parent=MS.gene71286 |
| chr3.4 | exon | 78134876 | 78135085 | 78134876 | ID=MS.gene71286.t1.exon1;Parent=MS.gene71286.t1 |
| chr3.4 | CDS | 78134876 | 78135085 | 78134876 | ID=cds.MS.gene71286.t1;Parent=MS.gene71286.t1 |
| chr3.4 | exon | 78135189 | 78135330 | 78135189 | ID=MS.gene71286.t1.exon2;Parent=MS.gene71286.t1 |
| chr3.4 | CDS | 78135189 | 78135330 | 78135189 | ID=cds.MS.gene71286.t1;Parent=MS.gene71286.t1 |
| chr3.4 | exon | 78135487 | 78135583 | 78135487 | ID=MS.gene71286.t1.exon3;Parent=MS.gene71286.t1 |
| chr3.4 | CDS | 78135487 | 78135583 | 78135487 | ID=cds.MS.gene71286.t1;Parent=MS.gene71286.t1 |
| chr3.4 | exon | 78135842 | 78135905 | 78135842 | ID=MS.gene71286.t1.exon4;Parent=MS.gene71286.t1 |
| chr3.4 | CDS | 78135842 | 78135905 | 78135842 | ID=cds.MS.gene71286.t1;Parent=MS.gene71286.t1 |
| Gene Sequence |
| Protein sequence |
