Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71527.t1 | RHN69093.1 | 74.7 | 83 | 18 | 1 | 8 | 90 | 154 | 233 | 7.80E-25 | 122.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71527.t1 | G7J9I4 | 74.7 | 83 | 18 | 1 | 8 | 90 | 133 | 212 | 5.6e-25 | 122.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene71527 | MS.gene77466 | PPI |
| MS.gene71527 | MS.gene044361 | PPI |
| MS.gene71527 | MS.gene71531 | PPI |
| MS.gene71527 | MS.gene71282 | PPI |
| MS.gene044363 | MS.gene71527 | PPI |
| MS.gene71527 | MS.gene71524 | PPI |
| MS.gene71527 | MS.gene71528 | PPI |
| MS.gene71527 | MS.gene045214 | PPI |
| MS.gene71527 | MS.gene77464 | PPI |
| MS.gene77472 | MS.gene71527 | PPI |
| MS.gene71527 | MS.gene044326 | PPI |
| MS.gene71527 | MS.gene79115 | PPI |
| MS.gene71527 | MS.gene70427 | PPI |
| MS.gene71527 | MS.gene70424 | PPI |
| MS.gene71527 | MS.gene70418 | PPI |
| MS.gene71527 | MS.gene049263 | PPI |
| MS.gene71527 | MS.gene044370 | PPI |
| MS.gene71527 | MS.gene70476 | PPI |
| MS.gene71527 | MS.gene25704 | PPI |
| MS.gene71527 | MS.gene77468 | PPI |
| MS.gene71527 | MS.gene044323 | PPI |
| MS.gene71527 | MS.gene71284 | PPI |
| MS.gene71527 | MS.gene044376 | PPI |
| MS.gene71527 | MS.gene70443 | PPI |
| MS.gene71527 | MS.gene70430 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71527.t1 | MTR_3g083150 | 74.699 | 83 | 18 | 1 | 8 | 90 | 133 | 212 | 1.08e-35 | 120 |
| MS.gene71527.t1 | MTR_3g083100 | 52.222 | 90 | 31 | 2 | 9 | 90 | 89 | 174 | 9.01e-22 | 84.0 |
| MS.gene71527.t1 | MTR_3g083080 | 47.368 | 95 | 36 | 5 | 1 | 89 | 119 | 205 | 1.52e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGTAGCATCTTCTGGTTT+TGG | 0.308463 | 3.4:-78268001 | None:intergenic |
| TGAACATGGGTAGCATCTTC+TGG | 0.357963 | 3.4:-78268007 | None:intergenic |
| TCGCCTCTCCAAGCTTGCTT+TGG | 0.392937 | 3.4:+78267770 | MS.gene71527:CDS |
| GAAGTACAATGCTGACAATC+AGG | 0.431538 | 3.4:+78267794 | MS.gene71527:CDS |
| CACGACTTGATCACAGGTTC+AGG | 0.456720 | 3.4:-78268082 | None:intergenic |
| GTACTTCTCCAAAGCAAGCT+TGG | 0.468678 | 3.4:-78267778 | None:intergenic |
| ATCATCATCGGTAATGTCCA+TGG | 0.485528 | 3.4:-78267748 | None:intergenic |
| CGGGCCTGAAAAGTTATAGC+AGG | 0.514776 | 3.4:-78268043 | None:intergenic |
| ACCCGTGTGCATATTCCTCC+TGG | 0.529882 | 3.4:+78267950 | MS.gene71527:CDS |
| AAAAGTTATAGCAGGGCAGT+TGG | 0.545343 | 3.4:-78268035 | None:intergenic |
| TGAATTTGATGTGCTCATCA+AGG | 0.546727 | 3.4:+78267925 | MS.gene71527:CDS |
| CTTGGAGAGGCGATCATCAT+CGG | 0.553632 | 3.4:-78267760 | None:intergenic |
| ATGGAACACGACTTGATCAC+AGG | 0.555050 | 3.4:-78268088 | None:intergenic |
| GTTATAGCAGGGCAGTTGGA+AGG | 0.582712 | 3.4:-78268031 | None:intergenic |
| CAGGCATACTGTCACAGACA+CGG | 0.585144 | 3.4:-78268063 | None:intergenic |
| GGCAGTTGGAAGGATGAACA+TGG | 0.590763 | 3.4:-78268021 | None:intergenic |
| TCATCATCGGTAATGTCCAT+GGG | 0.608264 | 3.4:-78267747 | None:intergenic |
| AGGCATACTGTCACAGACAC+GGG | 0.620581 | 3.4:-78268062 | None:intergenic |
| AAGGTAATATAATAAGTACC+AGG | 0.642014 | 3.4:-78267968 | None:intergenic |
| TCTCCAAAGCAAGCTTGGAG+AGG | 0.642137 | 3.4:-78267773 | None:intergenic |
| TACCAGGAGGAATATGCACA+CGG | 0.651827 | 3.4:-78267952 | None:intergenic |
| GGGCCTGAAAAGTTATAGCA+GGG | 0.658880 | 3.4:-78268042 | None:intergenic |
| GCAGTTGGAAGGATGAACAT+GGG | 0.704484 | 3.4:-78268020 | None:intergenic |
| GTAATATAATAAGTACCAGG+AGG | 0.711300 | 3.4:-78267965 | None:intergenic |
| ACCAGGAGGAATATGCACAC+GGG | 0.718548 | 3.4:-78267951 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGTTTCTCTTATTT+AGG | + | chr3.4:78267892-78267911 | MS.gene71527:intron | 15.0% |
| ! | AAGGTAATATAATAAGTACC+AGG | - | chr3.4:78267971-78267990 | None:intergenic | 25.0% |
| ! | ACATGGATATAATATAACAG+TGG | - | chr3.4:78267838-78267857 | None:intergenic | 25.0% |
| GATGAGTGAAAGAATAAACA+TGG | - | chr3.4:78267855-78267874 | None:intergenic | 30.0% | |
| GTAATATAATAAGTACCAGG+AGG | - | chr3.4:78267968-78267987 | None:intergenic | 30.0% | |
| TGGATATAATATAACAGTGG+TGG | - | chr3.4:78267835-78267854 | None:intergenic | 30.0% | |
| !!! | TGGTTTTGGTTTTGCTTTAA+AGG | - | chr3.4:78267990-78268009 | None:intergenic | 30.0% |
| TGAATTTGATGTGCTCATCA+AGG | + | chr3.4:78267925-78267944 | MS.gene71527:CDS | 35.0% | |
| AAAAGTTATAGCAGGGCAGT+TGG | - | chr3.4:78268038-78268057 | None:intergenic | 40.0% | |
| GAAGTACAATGCTGACAATC+AGG | + | chr3.4:78267794-78267813 | MS.gene71527:CDS | 40.0% | |
| ATGGAACACGACTTGATCAC+AGG | - | chr3.4:78268091-78268110 | None:intergenic | 45.0% | |
| GCAGTTGGAAGGATGAACAT+GGG | - | chr3.4:78268023-78268042 | None:intergenic | 45.0% | |
| GGGCCTGAAAAGTTATAGCA+GGG | - | chr3.4:78268045-78268064 | None:intergenic | 45.0% | |
| GTACTTCTCCAAAGCAAGCT+TGG | - | chr3.4:78267781-78267800 | None:intergenic | 45.0% | |
| TACCAGGAGGAATATGCACA+CGG | - | chr3.4:78267955-78267974 | None:intergenic | 45.0% | |
| TGAACATGGGTAGCATCTTC+TGG | - | chr3.4:78268010-78268029 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGGTGAACTAGTT+TGG | - | chr3.4:78267822-78267841 | None:intergenic | 45.0% |
| ! | CTGCCCTGCTATAACTTTTC+AGG | + | chr3.4:78268039-78268058 | MS.gene71527:CDS | 45.0% |
| !!! | TGGGTAGCATCTTCTGGTTT+TGG | - | chr3.4:78268004-78268023 | None:intergenic | 45.0% |
| ACCAGGAGGAATATGCACAC+GGG | - | chr3.4:78267954-78267973 | None:intergenic | 50.0% | |
| AGGCATACTGTCACAGACAC+GGG | - | chr3.4:78268065-78268084 | None:intergenic | 50.0% | |
| CACGACTTGATCACAGGTTC+AGG | - | chr3.4:78268085-78268104 | None:intergenic | 50.0% | |
| CAGGCATACTGTCACAGACA+CGG | - | chr3.4:78268066-78268085 | None:intergenic | 50.0% | |
| CGGGCCTGAAAAGTTATAGC+AGG | - | chr3.4:78268046-78268065 | None:intergenic | 50.0% | |
| CTTGGAGAGGCGATCATCAT+CGG | - | chr3.4:78267763-78267782 | None:intergenic | 50.0% | |
| GGCAGTTGGAAGGATGAACA+TGG | - | chr3.4:78268024-78268043 | None:intergenic | 50.0% | |
| GTTATAGCAGGGCAGTTGGA+AGG | - | chr3.4:78268034-78268053 | None:intergenic | 50.0% | |
| TCTCCAAAGCAAGCTTGGAG+AGG | - | chr3.4:78267776-78267795 | None:intergenic | 50.0% | |
| ACCCGTGTGCATATTCCTCC+TGG | + | chr3.4:78267950-78267969 | MS.gene71527:CDS | 55.0% | |
| ! | TCGCCTCTCCAAGCTTGCTT+TGG | + | chr3.4:78267770-78267789 | MS.gene71527:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 78267750 | 78268120 | 78267750 | ID=MS.gene71527 |
| chr3.4 | mRNA | 78267750 | 78268120 | 78267750 | ID=MS.gene71527.t1;Parent=MS.gene71527 |
| chr3.4 | exon | 78267750 | 78267815 | 78267750 | ID=MS.gene71527.t1.exon1;Parent=MS.gene71527.t1 |
| chr3.4 | CDS | 78267750 | 78267815 | 78267750 | ID=cds.MS.gene71527.t1;Parent=MS.gene71527.t1 |
| chr3.4 | exon | 78267914 | 78268120 | 78267914 | ID=MS.gene71527.t1.exon2;Parent=MS.gene71527.t1 |
| chr3.4 | CDS | 78267914 | 78268120 | 78267914 | ID=cds.MS.gene71527.t1;Parent=MS.gene71527.t1 |
| Gene Sequence |
| Protein sequence |
