AlfalfaGEDB | MS.gene71530

M. sativa cultivar XinJiangDaYe / MS.gene71530

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71530.t1	RHN69088.1	75.7	214	27	5	1	214	1	189	4.60E-76	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71530.t1	A0A396IWV3	75.7	214	27	5	1	214	1	189	3.3e-76	294.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene044363	MS.gene71530	PPI
MS.gene70430	MS.gene71530	PPI
MS.gene71530	MS.gene71282	PPI
MS.gene71530	MS.gene71524	PPI
MS.gene71530	MS.gene71528	PPI
MS.gene70418	MS.gene71530	PPI
MS.gene71530	MS.gene77464	PPI
MS.gene71530	MS.gene044326	PPI
MS.gene71530	MS.gene70427	PPI
MS.gene71530	MS.gene70424	PPI
MS.gene77472	MS.gene71530	PPI
MS.gene71530	MS.gene049263	PPI
MS.gene71530	MS.gene70476	PPI
MS.gene71530	MS.gene25704	PPI
MS.gene71530	MS.gene77468	PPI
MS.gene71530	MS.gene71531	PPI
MS.gene71530	MS.gene044323	PPI
MS.gene79115	MS.gene71530	PPI
MS.gene044370	MS.gene71530	PPI
MS.gene70443	MS.gene71530	PPI
MS.gene71530	MS.gene71284	PPI
MS.gene71530	MS.gene044376	PPI
MS.gene71530	MS.gene045214	PPI
MS.gene71530	MS.gene77466	PPI
MS.gene71530	MS.gene044361	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71530.t1	MTR_3g083030	53.975	239	76	8	1	214	1	230	4.62e-73	221
MS.gene71530.t1	MTR_3g083050	54.338	219	83	7	1	214	1	207	2.07e-64	198
MS.gene71530.t1	MTR_3g083080	48.858	219	92	6	1	213	1	205	4.17e-61	190
MS.gene71530.t1	MTR_3g086030	45.946	111	47	4	30	140	6	103	2.20e-21	86.7
MS.gene71530.t1	MTR_3g082340	34.483	232	123	10	1	213	1	222	2.58e-21	88.2
MS.gene71530.t1	MTR_3g082420	36.224	196	100	9	30	213	39	221	2.87e-21	88.2
MS.gene71530.t1	MTR_3g083150	33.784	222	129	8	1	214	1	212	1.33e-15	72.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 58 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGTTCCGGTTCCGGTTTC+AGG	0.174029	3.4:-78252259	None:intergenic
TCCAACTTCAGTTCCGGTTC+CGG	0.326780	3.4:-78252266	None:intergenic
CCTCTTTCATCGCAGCTATC+CGG	0.342132	3.4:-78252230	None:intergenic
TTCGGTTCCAACTTCAGTTC+CGG	0.351103	3.4:-78252272	None:intergenic
GAAATACAATACTGAGAATC+AGG	0.365773	3.4:+78252733	MS.gene71530:CDS
GCATGTTTAAGTTGCCGTTT+CGG	0.375760	3.4:-78252290	None:intergenic
CTCTTTCATCGCAGCTATCC+GGG	0.391793	3.4:-78252229	None:intergenic
TCCCCTCTGCGAGGCTGCGT+TGG	0.405826	3.4:+78252709	MS.gene71530:CDS
TGTGCCTGGAATGTTGTAAC+AGG	0.415678	3.4:-78252941	None:intergenic
CTGCCCTGTTACAACATTCC+AGG	0.418167	3.4:+78252937	MS.gene71530:CDS
TCGCTTCGAGTATAGGAATA+AGG	0.423727	3.4:+78252400	MS.gene71530:CDS
CCGAGAATTGCAGGATTAGT+TGG	0.424527	3.4:+78252638	MS.gene71530:intron
CGATCACATTTAAATATAAG+AGG	0.432066	3.4:-78252380	None:intergenic
CAACATTCCAGGCACAAGTC+TGG	0.445688	3.4:+78252948	MS.gene71530:CDS
CGCAGAGGGGAGTGAGTTGA+AGG	0.451519	3.4:-78252697	None:intergenic
TCGGATGACGCCGAACAAAC+AGG	0.454507	3.4:-78252201	None:intergenic
AATGTGATCGCTTCGAGTAT+AGG	0.463814	3.4:+78252393	MS.gene71530:CDS
GGATCCGATACCTGTTTGTT+CGG	0.471624	3.4:+78252191	MS.gene71530:CDS
AAGGCGAAGATCATCATTGA+TGG	0.480182	3.4:-78252678	None:intergenic
AAGAGGGAAGTGCCTGAAAC+CGG	0.483559	3.4:+78252247	MS.gene71530:CDS
ACCGGAACCGGAACTGAAGT+TGG	0.497592	3.4:+78252265	MS.gene71530:CDS
AATTGAAAGCCAAACGAAAA+CGG	0.504358	3.4:-78252535	None:intergenic
CGGCGTCATCCGACCTGCCC+CGG	0.523577	3.4:+78252211	MS.gene71530:CDS
CGCAGCTATCCGGGGCAGGT+CGG	0.524449	3.4:-78252220	None:intergenic
AACGGCAACTTAAACATGCA+TGG	0.528024	3.4:+78252294	MS.gene71530:CDS
TCATCGCAGCTATCCGGGGC+AGG	0.528179	3.4:-78252224	None:intergenic
GTTGTAACAGGGCAGCAGTT+GGG	0.528764	3.4:-78252929	None:intergenic
AACTGAAGTTGGAACCGAAA+CGG	0.529822	3.4:+78252276	MS.gene71530:CDS
ATACATACCACCAGGTCTCC+AGG	0.529976	3.4:-78252873	None:intergenic
CACCAACGCAGCCTCGCAGA+GGG	0.535103	3.4:-78252711	None:intergenic
CTGACATTGTGAAGACAACC+TGG	0.546641	3.4:+78252855	MS.gene71530:CDS
GAAGTGCCTGAAACCGGAAC+CGG	0.547790	3.4:+78252253	MS.gene71530:CDS
TGTTGTAACAGGGCAGCAGT+TGG	0.551781	3.4:-78252930	None:intergenic
CCGGATAGCTGCGATGAAAG+AGG	0.559882	3.4:+78252230	MS.gene71530:CDS
GAAGATCATCATTGATGGGA+AGG	0.563367	3.4:-78252673	None:intergenic
AGATCATCATTGATGGGAAG+GGG	0.573339	3.4:-78252671	None:intergenic
CGGATAGCTGCGATGAAAGA+GGG	0.573668	3.4:+78252231	MS.gene71530:CDS
ACGGCAACTTAAACATGCAT+GGG	0.577716	3.4:+78252295	MS.gene71530:CDS
CAGAGCAGTCACTAATCTCA+AGG	0.578303	3.4:-78252337	None:intergenic
GAGGCCTGTGTCTGAGTCTG+AGG	0.582177	3.4:+78252976	MS.gene71530:CDS
AAGATCATCATTGATGGGAA+GGG	0.584250	3.4:-78252672	None:intergenic
GACGCCGAACAAACAGGTAT+CGG	0.594685	3.4:-78252195	None:intergenic
GTGAAGACAACCTGGAGACC+TGG	0.604519	3.4:+78252863	MS.gene71530:CDS
AGGCGAAGATCATCATTGAT+GGG	0.608811	3.4:-78252677	None:intergenic
ATGACCTCAGACTCAGACAC+AGG	0.613733	3.4:-78252980	None:intergenic
GAGCTGTTTGTACTCAGCCA+TGG	0.614513	3.4:-78252456	None:intergenic
TCTTTCATCGCAGCTATCCG+GGG	0.623898	3.4:-78252228	None:intergenic
GTAACAGGGCAGCAGTTGGG+AGG	0.636023	3.4:-78252926	None:intergenic
TAAGGAGATTAAAGTCGCCA+TGG	0.636681	3.4:+78252439	MS.gene71530:CDS
ATATATAGATACGTACACTT+AGG	0.638669	3.4:-78252487	None:intergenic
TCACCAACGCAGCCTCGCAG+AGG	0.646731	3.4:-78252712	None:intergenic
TCAACTCACTCCCCTCTGCG+AGG	0.647226	3.4:+78252700	MS.gene71530:CDS
TACGTACACTTAGGCCGCGA+CGG	0.647938	3.4:-78252478	None:intergenic
GTAATATAATACATACCACC+AGG	0.693054	3.4:-78252881	None:intergenic
ACCAACGCAGCCTCGCAGAG+GGG	0.695672	3.4:-78252710	None:intergenic
GAGTACAAACAGCTCCGTCG+CGG	0.736504	3.4:+78252464	MS.gene71530:CDS
GTGCCTGGAATGTTGTAACA+GGG	0.754604	3.4:-78252940	None:intergenic
AAGACAACCTGGAGACCTGG+TGG	0.761347	3.4:+78252866	MS.gene71530:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATATAGATACGTACACTT+AGG	-	chr3.4:78252490-78252509	None:intergenic	25.0%
!	CGATCACATTTAAATATAAG+AGG	-	chr3.4:78252383-78252402	None:intergenic	25.0%
!!!	CTCTTACTTAATTTTGTTTC+TGG	+	chr3.4:78252590-78252609	MS.gene71530:intron	25.0%
	AATTGAAAGCCAAACGAAAA+CGG	-	chr3.4:78252538-78252557	None:intergenic	30.0%
	GAAATACAATACTGAGAATC+AGG	+	chr3.4:78252733-78252752	MS.gene71530:CDS	30.0%
	GTAATATAATACATACCACC+AGG	-	chr3.4:78252884-78252903	None:intergenic	30.0%
!	ATCTTGTTCTTTTGCTTGAA+AGG	-	chr3.4:78252906-78252925	None:intergenic	30.0%
	AAGATCATCATTGATGGGAA+GGG	-	chr3.4:78252675-78252694	None:intergenic	35.0%
	GGCTCAGATTCAAAAAGATA+AGG	+	chr3.4:78252421-78252440	MS.gene71530:CDS	35.0%
!	TTTTGCTTTCCGTTTTCGTT+TGG	+	chr3.4:78252526-78252545	MS.gene71530:intron	35.0%
	AACGGCAACTTAAACATGCA+TGG	+	chr3.4:78252294-78252313	MS.gene71530:CDS	40.0%
	AACTGAAGTTGGAACCGAAA+CGG	+	chr3.4:78252276-78252295	MS.gene71530:CDS	40.0%
	AAGGCGAAGATCATCATTGA+TGG	-	chr3.4:78252681-78252700	None:intergenic	40.0%
	AATGTGATCGCTTCGAGTAT+AGG	+	chr3.4:78252393-78252412	MS.gene71530:CDS	40.0%
	ACGGCAACTTAAACATGCAT+GGG	+	chr3.4:78252295-78252314	MS.gene71530:CDS	40.0%
	AGATCATCATTGATGGGAAG+GGG	-	chr3.4:78252674-78252693	None:intergenic	40.0%
	AGGCGAAGATCATCATTGAT+GGG	-	chr3.4:78252680-78252699	None:intergenic	40.0%
	CCAACTAATCCTGCAATTCT+CGG	-	chr3.4:78252641-78252660	None:intergenic	40.0%
	GAAGATCATCATTGATGGGA+AGG	-	chr3.4:78252676-78252695	None:intergenic	40.0%
	TAAGGAGATTAAAGTCGCCA+TGG	+	chr3.4:78252439-78252458	MS.gene71530:CDS	40.0%
	TCGCTTCGAGTATAGGAATA+AGG	+	chr3.4:78252400-78252419	MS.gene71530:CDS	40.0%
!!	GCATGTTTAAGTTGCCGTTT+CGG	-	chr3.4:78252293-78252312	None:intergenic	40.0%
	ACTAATCCTGCAATTCTCGG+AGG	-	chr3.4:78252638-78252657	None:intergenic	45.0%
	AGGCACAAGTCTGGAAAAAG+AGG	+	chr3.4:78252957-78252976	MS.gene71530:CDS	45.0%
	CAGAGCAGTCACTAATCTCA+AGG	-	chr3.4:78252340-78252359	None:intergenic	45.0%
	CTAATCCTGCAATTCTCGGA+GGG	-	chr3.4:78252637-78252656	None:intergenic	45.0%
	CTGACATTGTGAAGACAACC+TGG	+	chr3.4:78252855-78252874	MS.gene71530:CDS	45.0%
	GGATCCGATACCTGTTTGTT+CGG	+	chr3.4:78252191-78252210	MS.gene71530:CDS	45.0%
	GTGCCTGGAATGTTGTAACA+GGG	-	chr3.4:78252943-78252962	None:intergenic	45.0%
	TAATCCTGCAATTCTCGGAG+GGG	-	chr3.4:78252636-78252655	None:intergenic	45.0%
	TGTGCCTGGAATGTTGTAAC+AGG	-	chr3.4:78252944-78252963	None:intergenic	45.0%
	TTCGGTTCCAACTTCAGTTC+CGG	-	chr3.4:78252275-78252294	None:intergenic	45.0%
!	CCGAGAATTGCAGGATTAGT+TGG	+	chr3.4:78252638-78252657	MS.gene71530:intron	45.0%
!	TCTTTTTCCAGACTTGTGCC+TGG	-	chr3.4:78252958-78252977	None:intergenic	45.0%
	ATACATACCACCAGGTCTCC+AGG	-	chr3.4:78252876-78252895	None:intergenic	50.0%
	ATGACCTCAGACTCAGACAC+AGG	-	chr3.4:78252983-78253002	None:intergenic	50.0%
	CAACATTCCAGGCACAAGTC+TGG	+	chr3.4:78252948-78252967	MS.gene71530:CDS	50.0%
	CCTCTTTCATCGCAGCTATC+CGG	-	chr3.4:78252233-78252252	None:intergenic	50.0%
	CGGATAGCTGCGATGAAAGA+GGG	+	chr3.4:78252231-78252250	MS.gene71530:CDS	50.0%
	CTCTTTCATCGCAGCTATCC+GGG	-	chr3.4:78252232-78252251	None:intergenic	50.0%
	CTGCCCTGTTACAACATTCC+AGG	+	chr3.4:78252937-78252956	MS.gene71530:CDS	50.0%
	GACGCCGAACAAACAGGTAT+CGG	-	chr3.4:78252198-78252217	None:intergenic	50.0%
	GAGCTGTTTGTACTCAGCCA+TGG	-	chr3.4:78252459-78252478	None:intergenic	50.0%
	GTTGTAACAGGGCAGCAGTT+GGG	-	chr3.4:78252932-78252951	None:intergenic	50.0%
	TCCAACTTCAGTTCCGGTTC+CGG	-	chr3.4:78252269-78252288	None:intergenic	50.0%
	TCTTTCATCGCAGCTATCCG+GGG	-	chr3.4:78252231-78252250	None:intergenic	50.0%
	TGTTGTAACAGGGCAGCAGT+TGG	-	chr3.4:78252933-78252952	None:intergenic	50.0%
!!	AAGAGGGAAGTGCCTGAAAC+CGG	+	chr3.4:78252247-78252266	MS.gene71530:CDS	50.0%
	AAGACAACCTGGAGACCTGG+TGG	+	chr3.4:78252866-78252885	MS.gene71530:CDS	55.0%
	ACCGGAACCGGAACTGAAGT+TGG	+	chr3.4:78252265-78252284	MS.gene71530:CDS	55.0%
	ATCACCCCTCCGAGAATTGC+AGG	+	chr3.4:78252629-78252648	MS.gene71530:intron	55.0%
	CCGGATAGCTGCGATGAAAG+AGG	+	chr3.4:78252230-78252249	MS.gene71530:CDS	55.0%
	GAGTACAAACAGCTCCGTCG+CGG	+	chr3.4:78252464-78252483	MS.gene71530:CDS	55.0%
	GTGAAGACAACCTGGAGACC+TGG	+	chr3.4:78252863-78252882	MS.gene71530:CDS	55.0%
	TACGTACACTTAGGCCGCGA+CGG	-	chr3.4:78252481-78252500	None:intergenic	55.0%
	TCAGTTCCGGTTCCGGTTTC+AGG	-	chr3.4:78252262-78252281	None:intergenic	55.0%
	TCGGATGACGCCGAACAAAC+AGG	-	chr3.4:78252204-78252223	None:intergenic	55.0%
!	GAAGTGCCTGAAACCGGAAC+CGG	+	chr3.4:78252253-78252272	MS.gene71530:CDS	55.0%
	GAGGCCTGTGTCTGAGTCTG+AGG	+	chr3.4:78252976-78252995	MS.gene71530:CDS	60.0%
	GTAACAGGGCAGCAGTTGGG+AGG	-	chr3.4:78252929-78252948	None:intergenic	60.0%
	TCAACTCACTCCCCTCTGCG+AGG	+	chr3.4:78252700-78252719	MS.gene71530:CDS	60.0%
!	CGCAGAGGGGAGTGAGTTGA+AGG	-	chr3.4:78252700-78252719	None:intergenic	60.0%
	ACCAACGCAGCCTCGCAGAG+GGG	-	chr3.4:78252713-78252732	None:intergenic	65.0%
	CACCAACGCAGCCTCGCAGA+GGG	-	chr3.4:78252714-78252733	None:intergenic	65.0%
	TCACCAACGCAGCCTCGCAG+AGG	-	chr3.4:78252715-78252734	None:intergenic	65.0%
	TCATCGCAGCTATCCGGGGC+AGG	-	chr3.4:78252227-78252246	None:intergenic	65.0%
	CGCAGCTATCCGGGGCAGGT+CGG	-	chr3.4:78252223-78252242	None:intergenic	70.0%
	TCCCCTCTGCGAGGCTGCGT+TGG	+	chr3.4:78252709-78252728	MS.gene71530:CDS	70.0%
	CGGCGTCATCCGACCTGCCC+CGG	+	chr3.4:78252211-78252230	MS.gene71530:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	78252182	78253024	78252182	ID=MS.gene71530
chr3.4	mRNA	78252182	78253024	78252182	ID=MS.gene71530.t1;Parent=MS.gene71530
chr3.4	exon	78252182	78252542	78252182	ID=MS.gene71530.t1.exon1;Parent=MS.gene71530.t1
chr3.4	CDS	78252182	78252542	78252182	ID=cds.MS.gene71530.t1;Parent=MS.gene71530.t1
chr3.4	exon	78252651	78252758	78252651	ID=MS.gene71530.t1.exon2;Parent=MS.gene71530.t1
chr3.4	CDS	78252651	78252758	78252651	ID=cds.MS.gene71530.t1;Parent=MS.gene71530.t1
chr3.4	exon	78252849	78253024	78252849	ID=MS.gene71530.t1.exon3;Parent=MS.gene71530.t1
chr3.4	CDS	78252849	78253024	78252849	ID=cds.MS.gene71530.t1;Parent=MS.gene71530.t1

Gene Sequence

>MS.gene71530

ATGTCGAAGGGATCCGATACCTGTTTGTTCGGCGTCATCCGACCTGCCCCGGATAGCTGCGATGAAAGAGGGAAGTGCCTGAAACCGGAACCGGAACTGAAGTTGGAACCGAAACGGCAACTTAAACATGCATGGGAATCGAAACGCGTTAAAGACCTTGAGATTAGTGACTGCTCTGTGCATGACGATGACATTGAGCCTCTTATATTTAAATGTGATCGCTTCGAGTATAGGAATAAGGCTCAGATTCAAAAAGATAAGGAGATTAAAGTCGCCATGGCTGAGTACAAACAGCTCCGTCGCGGCCTAAGTGTACGTATCTATATATCTATCTATCTATCTCATTTTGCTTTCCGTTTTCGTTTGGCTTTCAATTTCATGTTTCACTTGTAATACAATAATAATAATCTCTTACTTAATTTTGTTTCTGGACAGAATTTTGATGCTATCACCCCTCCGAGAATTGCAGGATTAGTTGGTTGTATAGTACCCCTTCCCATCAATGATGATCTTCGCCTTCAACTCACTCCCCTCTGCGAGGCTGCGTTGGTGAAATACAATACTGAGAATCAGGCAAGTTCAATACTTTTTAGAGTAAACTTATGATGATTACAGCATATTTACTCATTCATTGATCAAATAATTAAGTATTCTTCTTAATTATTAGTGTTAGCTGACATTGTGAAGACAACCTGGAGACCTGGTGGTATGTATTATATTACCTTTCAAGCAAAAGAACAAGATCCTCCCAACTGCTGCCCTGTTACAACATTCCAGGCACAAGTCTGGAAAAAGAGGCCTGTGTCTGAGTCTGAGGTCATATCATGTGCCATCAAAACCTAA

Protein sequence

>MS.gene71530.t1

MSKGSDTCLFGVIRPAPDSCDERGKCLKPEPELKLEPKRQLKHAWESKRVKDLEISDCSVHDDDIEPLIFKCDRFEYRNKAQIQKDKEIKVAMAEYKQLRRGLSVRIYISIYLSHFAFRFRLVGCIVPLPINDDLRLQLTPLCEAALVKYNTENQAMLADIVKTTWRPGGMYYITFQAKEQDPPNCCPVTTFQAQVWKKRPVSESEVISCAIKT