Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73522.t1 | XP_003624883.1 | 95.5 | 110 | 5 | 0 | 5 | 114 | 94 | 203 | 1.20E-46 | 195.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73522.t1 | Q9M612 | 77.3 | 110 | 24 | 1 | 5 | 113 | 95 | 204 | 2.1e-36 | 152.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73522.t1 | G7L4T7 | 95.5 | 110 | 5 | 0 | 5 | 114 | 94 | 203 | 8.6e-47 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene73522 | 0.807044 | 6.13E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene73522 | 0.807463 | 4.99E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene73522 | 0.817134 | 3.86E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene73522 | 0.807719 | 4.41E-50 | -1.69E-46 |
| MS.gene051710 | MS.gene73522 | 0.828433 | 9.02E-55 | -1.69E-46 |
| MS.gene051719 | MS.gene73522 | 0.800826 | 1.20E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene73522 | 0.814725 | 1.33E-51 | -1.69E-46 |
| MS.gene052456 | MS.gene73522 | 0.800485 | 1.41E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene73522 | 0.803971 | 2.70E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene73522 | 0.816779 | 4.64E-52 | -1.69E-46 |
| MS.gene05288 | MS.gene73522 | 0.804399 | 2.20E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene73522 | 0.806133 | 9.54E-50 | -1.69E-46 |
| MS.gene053296 | MS.gene73522 | 0.800709 | 1.27E-48 | -1.69E-46 |
| MS.gene054236 | MS.gene73522 | 0.820534 | 6.52E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene73522 | 0.811885 | 5.60E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene73522 | 0.826131 | 3.21E-54 | -1.69E-46 |
| MS.gene054801 | MS.gene73522 | 0.854985 | 8.55E-62 | -1.69E-46 |
| MS.gene055357 | MS.gene73522 | 0.809701 | 1.66E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene73522 | 0.815698 | 8.09E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene73522 | 0.807231 | 5.59E-50 | -1.69E-46 |
| MS.gene055926 | MS.gene73522 | 0.811122 | 8.20E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene73522 | 0.810032 | 1.41E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene73522 | 0.804349 | 2.25E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene73522 | 0.803662 | 3.13E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene73522 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene73522 | 0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene73522 | 0.802938 | 4.42E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene73522 | 0.803719 | 3.05E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene73522 | 0.802869 | 4.57E-49 | -1.69E-46 |
| MS.gene060312 | MS.gene73522 | 0.806823 | 6.82E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene73522 | 0.802023 | 6.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73522.t1 | MTR_7g088680 | 95.455 | 110 | 5 | 0 | 5 | 114 | 94 | 203 | 1.92e-71 | 212 |
| MS.gene73522.t1 | MTR_3g092970 | 64.655 | 116 | 35 | 2 | 5 | 114 | 112 | 227 | 3.22e-41 | 136 |
| MS.gene73522.t1 | MTR_3g092980 | 53.659 | 123 | 36 | 2 | 5 | 114 | 106 | 220 | 1.70e-34 | 118 |
| MS.gene73522.t1 | MTR_3g092980 | 53.659 | 123 | 36 | 2 | 5 | 114 | 104 | 218 | 1.80e-34 | 118 |
| MS.gene73522.t1 | MTR_3g093750 | 53.571 | 112 | 41 | 3 | 5 | 114 | 425 | 527 | 1.70e-25 | 99.8 |
| MS.gene73522.t1 | MTR_3g093720 | 76.744 | 43 | 10 | 0 | 72 | 114 | 215 | 257 | 3.57e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73522.t1 | AT3G12390 | 74.107 | 112 | 27 | 1 | 5 | 114 | 92 | 203 | 2.23e-42 | 138 |
| MS.gene73522.t1 | AT5G13850 | 70.085 | 117 | 28 | 1 | 5 | 114 | 88 | 204 | 1.64e-40 | 133 |
| MS.gene73522.t1 | AT3G49470 | 58.621 | 116 | 42 | 1 | 5 | 114 | 102 | 217 | 4.12e-37 | 125 |
| MS.gene73522.t1 | AT4G10480 | 61.538 | 117 | 35 | 2 | 5 | 113 | 96 | 210 | 4.39e-35 | 120 |
| MS.gene73522.t1 | AT4G10480 | 61.538 | 117 | 35 | 2 | 5 | 113 | 97 | 211 | 4.50e-35 | 120 |
| MS.gene73522.t1 | AT1G33040 | 57.391 | 115 | 43 | 2 | 5 | 113 | 94 | 208 | 5.70e-35 | 119 |
| MS.gene73522.t1 | AT3G49470 | 57.009 | 107 | 40 | 1 | 5 | 105 | 102 | 208 | 9.87e-32 | 112 |
Find 26 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGACACCTACATTATATTT+AGG | 0.195015 | 4.4:+64730021 | MS.gene73522:CDS |
| GGAGCAATACCAGAGGATTC+TGG | 0.324111 | 4.4:-64730134 | None:intergenic |
| TCGTCTACATCCTCATCTTC+TGG | 0.324398 | 4.4:-64730155 | None:intergenic |
| GCACCCGCATTGGTCAAATT+AGG | 0.353452 | 4.4:-64730107 | None:intergenic |
| GCTTTAACAGCTCTTGATCT+TGG | 0.356165 | 4.4:-64730230 | None:intergenic |
| AGACATTGTTGCTGCCATTA+TGG | 0.359211 | 4.4:+64730271 | MS.gene73522:CDS |
| GTTGGACTCTTGAAAACATC+TGG | 0.370830 | 4.4:-64729996 | None:intergenic |
| TTAATTTGTTAGCTCCATAA+TGG | 0.425574 | 4.4:-64730285 | None:intergenic |
| CACCACCAACTCAATATCCT+TGG | 0.437037 | 4.4:-64730192 | None:intergenic |
| TTCTGGCTTCGCACCCGCAT+TGG | 0.469966 | 4.4:-64730117 | None:intergenic |
| TCAGGCTGCAGAGCAGTTCA+AGG | 0.476596 | 4.4:+64730082 | MS.gene73522:CDS |
| GAGCTCACAGCTACAAAGTC+AGG | 0.508240 | 4.4:+64730064 | MS.gene73522:CDS |
| AAAGCTCTCAAAGCTGTCAA+TGG | 0.508744 | 4.4:+64730248 | MS.gene73522:CDS |
| ATAATGTAGGTGTCAGAAGT+TGG | 0.511639 | 4.4:-64730014 | None:intergenic |
| TCCCAAGGATATTGAGTTGG+TGG | 0.515624 | 4.4:+64730190 | MS.gene73522:CDS |
| GCTCCTAATTTGACCAATGC+GGG | 0.519514 | 4.4:+64730104 | MS.gene73522:CDS |
| ACCACCAACTCAATATCCTT+GGG | 0.531886 | 4.4:-64730191 | None:intergenic |
| GGTGCGAAGCCAGAATCCTC+TGG | 0.535151 | 4.4:+64730125 | MS.gene73522:CDS |
| GATGAGGATGTAGACGAGAC+TGG | 0.538671 | 4.4:+64730161 | MS.gene73522:CDS |
| GAGTTGGTGGTGACTCAAGC+TGG | 0.556994 | 4.4:+64730203 | MS.gene73522:CDS |
| AGCTTTCCTAAATATAATGT+AGG | 0.569892 | 4.4:-64730027 | None:intergenic |
| CGAGACTGGTGTAGATCCCA+AGG | 0.607645 | 4.4:+64730175 | MS.gene73522:CDS |
| TGGTATTGCTCCAGAAGATG+AGG | 0.607813 | 4.4:+64730145 | MS.gene73522:CDS |
| AGATCCCAAGGATATTGAGT+TGG | 0.629225 | 4.4:+64730187 | MS.gene73522:CDS |
| GGCTCCTAATTTGACCAATG+CGG | 0.643666 | 4.4:+64730103 | MS.gene73522:CDS |
| ATCTTCTGGAGCAATACCAG+AGG | 0.670950 | 4.4:-64730141 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAAAAATAGTGAAAAT+AGG | + | chr4.4:64729656-64729675 | MS.gene73522:intron | 10.0% |
| !!! | AATATTTTTTCTTCTTCTTT+TGG | + | chr4.4:64729910-64729929 | MS.gene73522:intron | 15.0% |
| ! | AGCTTTCCTAAATATAATGT+AGG | - | chr4.4:64730030-64730049 | None:intergenic | 25.0% |
| ! | TATTCTTGTATAGACTAATC+TGG | - | chr4.4:64729786-64729805 | None:intergenic | 25.0% |
| !!! | TTAATTTGTTAGCTCCATAA+TGG | - | chr4.4:64730288-64730307 | None:intergenic | 25.0% |
| !!! | TTTATTTTTTGCATGAACTG+AGG | + | chr4.4:64729871-64729890 | MS.gene73522:intron | 25.0% |
| AGAGAATGACTACTATTAGA+GGG | - | chr4.4:64729707-64729726 | None:intergenic | 30.0% | |
| CAAAATACATGTACATTGTC+AGG | + | chr4.4:64729810-64729829 | MS.gene73522:intron | 30.0% | |
| CTGACACCTACATTATATTT+AGG | + | chr4.4:64730021-64730040 | MS.gene73522:CDS | 30.0% | |
| GAAGTCATTATCAAAAGATG+TGG | + | chr4.4:64729603-64729622 | MS.gene73522:intron | 30.0% | |
| TAGAGAATGACTACTATTAG+AGG | - | chr4.4:64729708-64729727 | None:intergenic | 30.0% | |
| ! | CTGACAATGTACATGTATTT+TGG | - | chr4.4:64729812-64729831 | None:intergenic | 30.0% |
| AAGTTTAGACTACATCCTTC+TGG | - | chr4.4:64729735-64729754 | None:intergenic | 35.0% | |
| ATAATGTAGGTGTCAGAAGT+TGG | - | chr4.4:64730017-64730036 | None:intergenic | 35.0% | |
| GTCATTCTCTATTAACCAGA+AGG | + | chr4.4:64729717-64729736 | MS.gene73522:intron | 35.0% | |
| GTCTAAACTTGAACATACTC+TGG | + | chr4.4:64729745-64729764 | MS.gene73522:intron | 35.0% | |
| ! | TAGGAGTACACAAGTATTAG+AGG | + | chr4.4:64729675-64729694 | MS.gene73522:intron | 35.0% |
| AAAGCTCTCAAAGCTGTCAA+TGG | + | chr4.4:64730248-64730267 | MS.gene73522:CDS | 40.0% | |
| ACCACCAACTCAATATCCTT+GGG | - | chr4.4:64730194-64730213 | None:intergenic | 40.0% | |
| AGACATTGTTGCTGCCATTA+TGG | + | chr4.4:64730271-64730290 | MS.gene73522:CDS | 40.0% | |
| AGATCCCAAGGATATTGAGT+TGG | + | chr4.4:64730187-64730206 | MS.gene73522:CDS | 40.0% | |
| ATGAACTGAGGCTACAATGA+TGG | + | chr4.4:64729883-64729902 | MS.gene73522:intron | 40.0% | |
| GCTTTAACAGCTCTTGATCT+TGG | - | chr4.4:64730233-64730252 | None:intergenic | 40.0% | |
| GTTGGACTCTTGAAAACATC+TGG | - | chr4.4:64729999-64730018 | None:intergenic | 40.0% | |
| TTGAAAGTCATGCAGAACAG+TGG | - | chr4.4:64729838-64729857 | None:intergenic | 40.0% | |
| CACCACCAACTCAATATCCT+TGG | - | chr4.4:64730195-64730214 | None:intergenic | 45.0% | |
| GCTCCTAATTTGACCAATGC+GGG | + | chr4.4:64730104-64730123 | MS.gene73522:CDS | 45.0% | |
| GGCTCCTAATTTGACCAATG+CGG | + | chr4.4:64730103-64730122 | MS.gene73522:CDS | 45.0% | |
| TCGTCTACATCCTCATCTTC+TGG | - | chr4.4:64730158-64730177 | None:intergenic | 45.0% | |
| TGGTATTGCTCCAGAAGATG+AGG | + | chr4.4:64730145-64730164 | MS.gene73522:CDS | 45.0% | |
| !! | ATCTTCTGGAGCAATACCAG+AGG | - | chr4.4:64730144-64730163 | None:intergenic | 45.0% |
| !! | TCCCAAGGATATTGAGTTGG+TGG | + | chr4.4:64730190-64730209 | MS.gene73522:CDS | 45.0% |
| GAGCTCACAGCTACAAAGTC+AGG | + | chr4.4:64730064-64730083 | MS.gene73522:CDS | 50.0% | |
| GATGAGGATGTAGACGAGAC+TGG | + | chr4.4:64730161-64730180 | MS.gene73522:CDS | 50.0% | |
| GGAGCAATACCAGAGGATTC+TGG | - | chr4.4:64730137-64730156 | None:intergenic | 50.0% | |
| ! | GCACCCGCATTGGTCAAATT+AGG | - | chr4.4:64730110-64730129 | None:intergenic | 50.0% |
| CGAGACTGGTGTAGATCCCA+AGG | + | chr4.4:64730175-64730194 | MS.gene73522:CDS | 55.0% | |
| TCAGGCTGCAGAGCAGTTCA+AGG | + | chr4.4:64730082-64730101 | MS.gene73522:CDS | 55.0% | |
| !! | GAGTTGGTGGTGACTCAAGC+TGG | + | chr4.4:64730203-64730222 | MS.gene73522:CDS | 55.0% |
| GGTGCGAAGCCAGAATCCTC+TGG | + | chr4.4:64730125-64730144 | MS.gene73522:CDS | 60.0% | |
| !! | TTCTGGCTTCGCACCCGCAT+TGG | - | chr4.4:64730120-64730139 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64729551 | 64730307 | 64729551 | ID=MS.gene73522 |
| chr4.4 | mRNA | 64729551 | 64730307 | 64729551 | ID=MS.gene73522.t1;Parent=MS.gene73522 |
| chr4.4 | exon | 64729551 | 64729562 | 64729551 | ID=MS.gene73522.t1.exon1;Parent=MS.gene73522.t1 |
| chr4.4 | CDS | 64729551 | 64729562 | 64729551 | ID=cds.MS.gene73522.t1;Parent=MS.gene73522.t1 |
| chr4.4 | exon | 64729975 | 64730307 | 64729975 | ID=MS.gene73522.t1.exon2;Parent=MS.gene73522.t1 |
| chr4.4 | CDS | 64729975 | 64730307 | 64729975 | ID=cds.MS.gene73522.t1;Parent=MS.gene73522.t1 |
| Gene Sequence |
| Protein sequence |
