Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73915.t1 | RHN69057.1 | 76.8 | 138 | 32 | 0 | 1 | 138 | 105 | 242 | 1.60E-53 | 218.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73915.t1 | G7J851 | 76.8 | 138 | 32 | 0 | 1 | 138 | 86 | 223 | 1.2e-53 | 218.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene73915 | MS.gene70418 | PPI |
| MS.gene73915 | MS.gene049263 | PPI |
| MS.gene73915 | MS.gene044370 | PPI |
| MS.gene73915 | MS.gene70476 | PPI |
| MS.gene73915 | MS.gene25704 | PPI |
| MS.gene73915 | MS.gene77468 | PPI |
| MS.gene73915 | MS.gene71531 | PPI |
| MS.gene73915 | MS.gene044323 | PPI |
| MS.gene77472 | MS.gene73915 | PPI |
| MS.gene73915 | MS.gene71284 | PPI |
| MS.gene73915 | MS.gene044376 | PPI |
| MS.gene73915 | MS.gene70443 | PPI |
| MS.gene73915 | MS.gene70430 | PPI |
| MS.gene73915 | MS.gene045214 | PPI |
| MS.gene73915 | MS.gene77466 | PPI |
| MS.gene73915 | MS.gene044361 | PPI |
| MS.gene73915 | MS.gene71524 | PPI |
| MS.gene73915 | MS.gene71528 | PPI |
| MS.gene73915 | MS.gene70424 | PPI |
| MS.gene73915 | MS.gene77464 | PPI |
| MS.gene73915 | MS.gene71282 | PPI |
| MS.gene73915 | MS.gene044326 | PPI |
| MS.gene73915 | MS.gene79115 | PPI |
| MS.gene73915 | MS.gene70427 | PPI |
| MS.gene044363 | MS.gene73915 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73915.t1 | MTR_3g082420 | 79.137 | 139 | 27 | 2 | 1 | 138 | 85 | 222 | 5.58e-74 | 220 |
| MS.gene73915.t1 | MTR_3g082340 | 79.137 | 139 | 27 | 2 | 1 | 138 | 86 | 223 | 1.03e-73 | 219 |
| MS.gene73915.t1 | MTR_3g080680 | 49.645 | 141 | 63 | 4 | 1 | 138 | 64 | 199 | 1.22e-36 | 124 |
| MS.gene73915.t1 | MTR_3g083050 | 43.382 | 136 | 61 | 7 | 5 | 137 | 84 | 206 | 4.63e-19 | 79.7 |
| MS.gene73915.t1 | MTR_3g083030 | 43.382 | 136 | 61 | 7 | 5 | 137 | 107 | 229 | 6.84e-19 | 79.7 |
| MS.gene73915.t1 | MTR_8g461300 | 38.889 | 144 | 79 | 3 | 1 | 137 | 57 | 198 | 9.08e-19 | 78.6 |
| MS.gene73915.t1 | MTR_4g055800 | 40.650 | 123 | 58 | 3 | 1 | 121 | 164 | 273 | 2.89e-16 | 73.6 |
| MS.gene73915.t1 | MTR_3g083080 | 35.338 | 133 | 77 | 4 | 5 | 137 | 82 | 205 | 1.98e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTTTCCAGGCTCAAGTA+TGG | 0.302357 | 3.3:+69285762 | MS.gene73915:CDS |
| CCCTGCTTTAACAACTTTCC+AGG | 0.345313 | 3.3:+69285751 | MS.gene73915:CDS |
| CCGCCACATAACAGTGTTCT+TGG | 0.391644 | 3.3:-69285409 | None:intergenic |
| TGAACTCTCCCTGCTTGCAT+TGG | 0.445183 | 3.3:+69285477 | MS.gene73915:CDS |
| GCCTGGAAAGTTGTTAAAGC+AGG | 0.451262 | 3.3:-69285752 | None:intergenic |
| ATCGGTTATGAAGAGGGGTT+GGG | 0.467286 | 3.3:-69285434 | None:intergenic |
| TGTAATATTCCAATGCAAGC+AGG | 0.487476 | 3.3:-69285486 | None:intergenic |
| CACCGTCCCATACTTGAGCC+TGG | 0.497315 | 3.3:-69285769 | None:intergenic |
| TAACAGTGTTCTTGGCGGAC+GGG | 0.498416 | 3.3:-69285401 | None:intergenic |
| ACTTGATGAAACTCAAATGT+TGG | 0.500719 | 3.3:-69285632 | None:intergenic |
| CATCGGTTATGAAGAGGGGT+TGG | 0.502034 | 3.3:-69285435 | None:intergenic |
| AACAGTGTTCTTGGCGGACG+GGG | 0.511969 | 3.3:-69285400 | None:intergenic |
| ATCAGCAATACCAACATCAT+AGG | 0.513179 | 3.3:-69285666 | None:intergenic |
| ATCTTCATCGGTTATGAAGA+GGG | 0.524106 | 3.3:-69285440 | None:intergenic |
| AACTTTCCAGGCTCAAGTAT+GGG | 0.533504 | 3.3:+69285763 | MS.gene73915:CDS |
| CAGGGAGAGTTCATCAAGAG+TGG | 0.544597 | 3.3:-69285467 | None:intergenic |
| AGGGAGAGTTCATCAAGAGT+GGG | 0.550261 | 3.3:-69285466 | None:intergenic |
| AAGAGTGGGGCGATCTTCAT+CGG | 0.559401 | 3.3:-69285452 | None:intergenic |
| ATAACAGTGTTCTTGGCGGA+CGG | 0.562366 | 3.3:-69285402 | None:intergenic |
| TATGATGTTGGTATTGCTGA+TGG | 0.567223 | 3.3:+69285668 | MS.gene73915:CDS |
| CTTGCATTGGAATATTACAA+TGG | 0.578867 | 3.3:+69285490 | MS.gene73915:CDS |
| GTAATATTCCAATGCAAGCA+GGG | 0.591856 | 3.3:-69285485 | None:intergenic |
| CCACATAACAGTGTTCTTGG+CGG | 0.600956 | 3.3:-69285406 | None:intergenic |
| CCTGGAAAGTTGTTAAAGCA+GGG | 0.605506 | 3.3:-69285751 | None:intergenic |
| AGTTGTTAAAGCAGGGCTGT+CGG | 0.619352 | 3.3:-69285744 | None:intergenic |
| CCGCCAAGAACACTGTTATG+TGG | 0.619826 | 3.3:+69285406 | MS.gene73915:CDS |
| GATCTTCATCGGTTATGAAG+AGG | 0.624382 | 3.3:-69285441 | None:intergenic |
| GTCAAGTGTACCTATGATGT+TGG | 0.629074 | 3.3:+69285656 | MS.gene73915:CDS |
| TCTTCATCGGTTATGAAGAG+GGG | 0.634517 | 3.3:-69285439 | None:intergenic |
| TTCCAGGCTCAAGTATGGGA+CGG | 0.638739 | 3.3:+69285767 | MS.gene73915:CDS |
| ACAGTGTTCTTGGCGGACGG+GGG | 0.652393 | 3.3:-69285399 | None:intergenic |
| TAAACTCATGAAGAAACTAG+AGG | 0.657097 | 3.3:+69285221 | MS.gene73915:CDS |
| GGGAGAGTTCATCAAGAGTG+GGG | 0.657488 | 3.3:-69285465 | None:intergenic |
| CCAAGAACACTGTTATGTGG+CGG | 0.693885 | 3.3:+69285409 | MS.gene73915:CDS |
| GAGCATCTAGAGCGTTCTCG+CGG | 0.715724 | 3.3:+69285255 | MS.gene73915:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCTTCTTAATTAATTATT+AGG | + | chr3.3:69285607-69285626 | MS.gene73915:intron | 15.0% |
| !! | CAATGGAAAAAATAATAATC+AGG | + | chr3.3:69285507-69285526 | MS.gene73915:CDS | 20.0% |
| !!! | TAATTAATTTTGATTCATCC+TGG | + | chr3.3:69285348-69285367 | MS.gene73915:intron | 20.0% |
| ACTTGATGAAACTCAAATGT+TGG | - | chr3.3:69285635-69285654 | None:intergenic | 30.0% | |
| CTTGCATTGGAATATTACAA+TGG | + | chr3.3:69285490-69285509 | MS.gene73915:CDS | 30.0% | |
| TAAACTCATGAAGAAACTAG+AGG | + | chr3.3:69285221-69285240 | MS.gene73915:CDS | 30.0% | |
| !! | GAAGTGTCTTCTTTTGATAT+TGG | - | chr3.3:69285725-69285744 | None:intergenic | 30.0% |
| !!! | TGATATTGGTTTTGCTTGAA+AGG | - | chr3.3:69285711-69285730 | None:intergenic | 30.0% |
| ATCAGCAATACCAACATCAT+AGG | - | chr3.3:69285669-69285688 | None:intergenic | 35.0% | |
| GTAATATTCCAATGCAAGCA+GGG | - | chr3.3:69285488-69285507 | None:intergenic | 35.0% | |
| TATGATGTTGGTATTGCTGA+TGG | + | chr3.3:69285668-69285687 | MS.gene73915:CDS | 35.0% | |
| TGTAATATTCCAATGCAAGC+AGG | - | chr3.3:69285489-69285508 | None:intergenic | 35.0% | |
| ! | ATCTTCATCGGTTATGAAGA+GGG | - | chr3.3:69285443-69285462 | None:intergenic | 35.0% |
| AACTTTCCAGGCTCAAGTAT+GGG | + | chr3.3:69285763-69285782 | MS.gene73915:CDS | 40.0% | |
| CCTGGAAAGTTGTTAAAGCA+GGG | - | chr3.3:69285754-69285773 | None:intergenic | 40.0% | |
| GTCAAGTGTACCTATGATGT+TGG | + | chr3.3:69285656-69285675 | MS.gene73915:CDS | 40.0% | |
| ! | GATCTTCATCGGTTATGAAG+AGG | - | chr3.3:69285444-69285463 | None:intergenic | 40.0% |
| ! | TCTTCATCGGTTATGAAGAG+GGG | - | chr3.3:69285442-69285461 | None:intergenic | 40.0% |
| ACACCTGTATTGTGTTGTCC+AGG | - | chr3.3:69285369-69285388 | None:intergenic | 45.0% | |
| AGGGAGAGTTCATCAAGAGT+GGG | - | chr3.3:69285469-69285488 | None:intergenic | 45.0% | |
| AGTTGTTAAAGCAGGGCTGT+CGG | - | chr3.3:69285747-69285766 | None:intergenic | 45.0% | |
| ATAACAGTGTTCTTGGCGGA+CGG | - | chr3.3:69285405-69285424 | None:intergenic | 45.0% | |
| ATAGACGCGTACGTACACTT+AGG | - | chr3.3:69285281-69285300 | None:intergenic | 45.0% | |
| ATCGGTTATGAAGAGGGGTT+GGG | - | chr3.3:69285437-69285456 | None:intergenic | 45.0% | |
| CAACTTTCCAGGCTCAAGTA+TGG | + | chr3.3:69285762-69285781 | MS.gene73915:CDS | 45.0% | |
| CATCCTGGACAACACAATAC+AGG | + | chr3.3:69285363-69285382 | MS.gene73915:intron | 45.0% | |
| CCAAGAACACTGTTATGTGG+CGG | + | chr3.3:69285409-69285428 | MS.gene73915:CDS | 45.0% | |
| CCACATAACAGTGTTCTTGG+CGG | - | chr3.3:69285409-69285428 | None:intergenic | 45.0% | |
| CCCTGCTTTAACAACTTTCC+AGG | + | chr3.3:69285751-69285770 | MS.gene73915:CDS | 45.0% | |
| GCCTGGAAAGTTGTTAAAGC+AGG | - | chr3.3:69285755-69285774 | None:intergenic | 45.0% | |
| AAGAGTGGGGCGATCTTCAT+CGG | - | chr3.3:69285455-69285474 | None:intergenic | 50.0% | |
| CAGGGAGAGTTCATCAAGAG+TGG | - | chr3.3:69285470-69285489 | None:intergenic | 50.0% | |
| CCGCCAAGAACACTGTTATG+TGG | + | chr3.3:69285406-69285425 | MS.gene73915:CDS | 50.0% | |
| CCGCCACATAACAGTGTTCT+TGG | - | chr3.3:69285412-69285431 | None:intergenic | 50.0% | |
| GGGAGAGTTCATCAAGAGTG+GGG | - | chr3.3:69285468-69285487 | None:intergenic | 50.0% | |
| TGAACTCTCCCTGCTTGCAT+TGG | + | chr3.3:69285477-69285496 | MS.gene73915:CDS | 50.0% | |
| TTCCAGGCTCAAGTATGGGA+CGG | + | chr3.3:69285767-69285786 | MS.gene73915:CDS | 50.0% | |
| ! | CATCGGTTATGAAGAGGGGT+TGG | - | chr3.3:69285438-69285457 | None:intergenic | 50.0% |
| ! | TAACAGTGTTCTTGGCGGAC+GGG | - | chr3.3:69285404-69285423 | None:intergenic | 50.0% |
| ! | AACAGTGTTCTTGGCGGACG+GGG | - | chr3.3:69285403-69285422 | None:intergenic | 55.0% |
| ! | GAGCATCTAGAGCGTTCTCG+CGG | + | chr3.3:69285255-69285274 | MS.gene73915:CDS | 55.0% |
| CACCGTCCCATACTTGAGCC+TGG | - | chr3.3:69285772-69285791 | None:intergenic | 60.0% | |
| ! | ACAGTGTTCTTGGCGGACGG+GGG | - | chr3.3:69285402-69285421 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69285219 | 69285835 | 69285219 | ID=MS.gene73915 |
| chr3.3 | mRNA | 69285219 | 69285835 | 69285219 | ID=MS.gene73915.t1;Parent=MS.gene73915 |
| chr3.3 | exon | 69285219 | 69285284 | 69285219 | ID=MS.gene73915.t1.exon1;Parent=MS.gene73915.t1 |
| chr3.3 | CDS | 69285219 | 69285284 | 69285219 | ID=cds.MS.gene73915.t1;Parent=MS.gene73915.t1 |
| chr3.3 | exon | 69285385 | 69285528 | 69285385 | ID=MS.gene73915.t1.exon2;Parent=MS.gene73915.t1 |
| chr3.3 | CDS | 69285385 | 69285528 | 69285385 | ID=cds.MS.gene73915.t1;Parent=MS.gene73915.t1 |
| chr3.3 | exon | 69285629 | 69285835 | 69285629 | ID=MS.gene73915.t1.exon3;Parent=MS.gene73915.t1 |
| chr3.3 | CDS | 69285629 | 69285835 | 69285629 | ID=cds.MS.gene73915.t1;Parent=MS.gene73915.t1 |
| Gene Sequence |
| Protein sequence |
