AlfalfaGEDB | MS.gene74084

M. sativa cultivar XinJiangDaYe / MS.gene74084

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF13302.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74084.t1	XP_003621901.1	94.6	147	8	0	2	148	30	176	3.20E-76	294.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74084.t1	P05332	40.4	89	52	1	51	139	85	172	3.4e-10	66.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74084.t1	G7KXV4	94.6	147	8	0	2	148	30	176	2.3e-76	294.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene74084.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene74084	0.855158	7.61E-62	-1.69E-46
MS.gene049915	MS.gene74084	0.804442	2.16E-49	-1.69E-46
MS.gene050301	MS.gene74084	0.807565	4.75E-50	-1.69E-46
MS.gene051626	MS.gene74084	0.814267	1.68E-51	-1.69E-46
MS.gene051662	MS.gene74084	0.800542	1.37E-48	-1.69E-46
MS.gene051663	MS.gene74084	0.805513	1.29E-49	-1.69E-46
MS.gene051860	MS.gene74084	0.801559	8.50E-49	-1.69E-46
MS.gene052178	MS.gene74084	0.824998	5.96E-54	-1.69E-46
MS.gene053074	MS.gene74084	0.805568	1.25E-49	-1.69E-46
MS.gene053836	MS.gene74084	0.845159	4.83E-59	-1.69E-46
MS.gene054184	MS.gene74084	0.836634	8.34E-57	-1.69E-46
MS.gene054185	MS.gene74084	0.843176	1.65E-58	-1.69E-46
MS.gene055133	MS.gene74084	0.83685	7.35E-57	-1.69E-46
MS.gene055277	MS.gene74084	0.803477	3.42E-49	-1.69E-46
MS.gene055422	MS.gene74084	0.834035	3.78E-56	-1.69E-46
MS.gene056875	MS.gene74084	0.805131	1.55E-49	-1.69E-46
MS.gene057439	MS.gene74084	0.816185	6.30E-52	-1.69E-46
MS.gene057754	MS.gene74084	0.832877	7.36E-56	-1.69E-46
MS.gene05811	MS.gene74084	0.82075	5.81E-53	-1.69E-46
MS.gene058168	MS.gene74084	0.805162	1.53E-49	-1.69E-46
MS.gene058845	MS.gene74084	0.811112	8.24E-51	-1.69E-46
MS.gene05907	MS.gene74084	0.81075	9.87E-51	-1.69E-46
MS.gene059518	MS.gene74084	0.825138	5.52E-54	-1.69E-46
MS.gene05984	MS.gene74084	0.813872	2.05E-51	-1.69E-46
MS.gene059894	MS.gene74084	0.801561	8.50E-49	-1.69E-46
MS.gene06037	MS.gene74084	0.820967	5.19E-53	-1.69E-46
MS.gene060476	MS.gene74084	0.835079	2.07E-56	-1.69E-46
MS.gene061049	MS.gene74084	0.834673	2.62E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74084.t1	MTR_7g024780	94.558	147	8	0	2	148	30	176	3.95e-104	295
MS.gene74084.t1	MTR_7g024765	67.347	147	47	1	2	148	30	175	1.94e-71	212
MS.gene74084.t1	MTR_7g024760	67.361	144	46	1	2	145	30	172	1.16e-67	202
MS.gene74084.t1	MTR_7g114410	62.585	147	54	1	3	148	31	177	1.33e-63	192
MS.gene74084.t1	MTR_7g114410	62.500	128	47	1	3	129	31	158	3.74e-54	168
MS.gene74084.t1	MTR_3g463610	48.276	145	71	2	2	142	29	173	9.71e-44	143
MS.gene74084.t1	MTR_5g006510	45.255	137	71	2	10	142	52	188	3.03e-33	115
MS.gene74084.t1	MTR_1g103150	41.958	143	81	2	2	142	45	187	4.86e-32	112
MS.gene74084.t1	MTR_1g103110	42.143	140	79	2	2	139	45	184	5.42e-31	110
MS.gene74084.t1	MTR_5g006470	40.559	143	83	2	2	142	43	185	7.59e-31	109
MS.gene74084.t1	MTR_5g006460	44.526	137	69	5	13	142	54	190	5.97e-30	107
MS.gene74084.t1	MTR_5g006450	40.690	145	82	2	2	142	43	187	1.45e-29	106
MS.gene74084.t1	MTR_5g006520	39.189	148	80	3	2	142	1761	1905	3.38e-26	103

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74084.t1	AT3G22560	45.455	143	75	2	3	142	30	172	1.02e-37	127
MS.gene74084.t1	AT2G32020	40.690	145	81	3	2	144	41	182	6.16e-36	122
MS.gene74084.t1	AT2G32030	39.583	144	82	3	3	144	47	187	1.39e-35	122

Find 35 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCTATTGGATTCAACTAT+TGG	0.220333	7.4:+76597481	MS.gene74084:CDS
TGCTATTGGATTCAACTATT+GGG	0.279552	7.4:+76597482	MS.gene74084:CDS
TGAGAAGTATAAAGCAGATA+TGG	0.323753	7.4:+76597455	MS.gene74084:CDS
ACCACTCCATTGGAATGGTT+TGG	0.355259	7.4:+76597412	MS.gene74084:CDS
GCAGATATGGGTTATGCTAT+TGG	0.359042	7.4:+76597468	MS.gene74084:CDS
AGCTTGCAACCTTCTCAAAT+CGG	0.366599	7.4:-76597565	None:intergenic
TAATTCAAAGAAAGAAGCTT+TGG	0.384761	7.4:+76597335	MS.gene74084:CDS
CATAGCTTCTCAGAGGGTAT+TGG	0.423997	7.4:+76597605	MS.gene74084:CDS
GCTTGCAACCTTCTCAAATC+GGG	0.427428	7.4:-76597564	None:intergenic
AAGAATCTTCACTGCATTAG+TGG	0.453856	7.4:-76597517	None:intergenic
CAAAGAGGGTTTGCTCAGAA+AGG	0.466394	7.4:+76597644	MS.gene74084:CDS
TTCTCAAATCGGGGAACTCA+TGG	0.488747	7.4:-76597554	None:intergenic
CAGAGGGTATTGGAAAAGCT+AGG	0.497928	7.4:+76597615	MS.gene74084:CDS
GGTCATCAAGACATATTGAT+TGG	0.500106	7.4:-76597392	None:intergenic
GAGAAGTATAAAGCAGATAT+GGG	0.502094	7.4:+76597456	MS.gene74084:CDS
TGTCTTGATGACCACTCCAT+TGG	0.510495	7.4:+76597402	MS.gene74084:CDS
GGATTCAACTATTGGGGTCA+AGG	0.521820	7.4:+76597489	MS.gene74084:CDS
TGATGACCACTCCATTGGAA+TGG	0.535636	7.4:+76597407	MS.gene74084:CDS
AGACATATTGATTGGCGTAA+GGG	0.538608	7.4:-76597384	None:intergenic
GCTATTGGATTCAACTATTG+GGG	0.539184	7.4:+76597483	MS.gene74084:CDS
GACATATTGATTGGCGTAAG+GGG	0.541235	7.4:-76597383	None:intergenic
AAGACATATTGATTGGCGTA+AGG	0.551674	7.4:-76597385	None:intergenic
ATGAGTTCCCCGATTTGAGA+AGG	0.555146	7.4:+76597556	MS.gene74084:CDS
AATACTAACTAAACAACAGA+AGG	0.576587	7.4:-76597732	None:intergenic
AAGCTAGGGTTCTACAAAGA+GGG	0.577948	7.4:+76597630	MS.gene74084:CDS
AAGAATCCAAACCATTCCAA+TGG	0.587139	7.4:-76597418	None:intergenic
AGAGGGTATTGGAAAAGCTA+GGG	0.597985	7.4:+76597616	MS.gene74084:CDS
TACTTCTCATCATTAGCATG+AGG	0.611961	7.4:-76597441	None:intergenic
AGTAACTGAGCATACTCGAT+GGG	0.615385	7.4:+76597305	MS.gene74084:CDS
TCCAAACCATTCCAATGGAG+TGG	0.617327	7.4:-76597413	None:intergenic
AAAGCTAGGGTTCTACAAAG+AGG	0.630805	7.4:+76597629	MS.gene74084:CDS
CTTGCAACCTTCTCAAATCG+GGG	0.653528	7.4:-76597563	None:intergenic
GAGTAACTGAGCATACTCGA+TGG	0.655051	7.4:+76597304	MS.gene74084:CDS
TAGAGAACATAGCTTCTCAG+AGG	0.672958	7.4:+76597598	MS.gene74084:CDS
AGAGAACATAGCTTCTCAGA+GGG	0.687021	7.4:+76597599	MS.gene74084:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAAGGTTTTTTATTTTAA+AGG	+	chr7.4:76597660-76597679	MS.gene74084:CDS	15.0%
!!!	TCTATATTTTTAGCTTTCTT+TGG	+	chr7.4:76597697-76597716	MS.gene74084:CDS	20.0%
!!!	TTATTTTAAAGGAAACTTTG+TGG	+	chr7.4:76597671-76597690	MS.gene74084:CDS	20.0%
!	TAATTCAAAGAAAGAAGCTT+TGG	+	chr7.4:76597335-76597354	MS.gene74084:CDS	25.0%
	ATGCTATTGGATTCAACTAT+TGG	+	chr7.4:76597481-76597500	MS.gene74084:CDS	30.0%
	TGCTATTGGATTCAACTATT+GGG	+	chr7.4:76597482-76597501	MS.gene74084:CDS	30.0%
!	GAGAAGTATAAAGCAGATAT+GGG	+	chr7.4:76597456-76597475	MS.gene74084:CDS	30.0%
!	TGAGAAGTATAAAGCAGATA+TGG	+	chr7.4:76597455-76597474	MS.gene74084:CDS	30.0%
	AAGAATCCAAACCATTCCAA+TGG	-	chr7.4:76597421-76597440	None:intergenic	35.0%
	AAGAATCTTCACTGCATTAG+TGG	-	chr7.4:76597520-76597539	None:intergenic	35.0%
	GCTATTGGATTCAACTATTG+GGG	+	chr7.4:76597483-76597502	MS.gene74084:CDS	35.0%
	GGTCATCAAGACATATTGAT+TGG	-	chr7.4:76597395-76597414	None:intergenic	35.0%
	TACTTCTCATCATTAGCATG+AGG	-	chr7.4:76597444-76597463	None:intergenic	35.0%
!	AAGACATATTGATTGGCGTA+AGG	-	chr7.4:76597388-76597407	None:intergenic	35.0%
!	AGACATATTGATTGGCGTAA+GGG	-	chr7.4:76597387-76597406	None:intergenic	35.0%
	AAAGCTAGGGTTCTACAAAG+AGG	+	chr7.4:76597629-76597648	MS.gene74084:CDS	40.0%
	AAGCTAGGGTTCTACAAAGA+GGG	+	chr7.4:76597630-76597649	MS.gene74084:CDS	40.0%
	AGAGAACATAGCTTCTCAGA+GGG	+	chr7.4:76597599-76597618	MS.gene74084:CDS	40.0%
	AGCTTGCAACCTTCTCAAAT+CGG	-	chr7.4:76597568-76597587	None:intergenic	40.0%
	AGTAACTGAGCATACTCGAT+GGG	+	chr7.4:76597305-76597324	MS.gene74084:CDS	40.0%
	TAGAGAACATAGCTTCTCAG+AGG	+	chr7.4:76597598-76597617	MS.gene74084:CDS	40.0%
!	GACATATTGATTGGCGTAAG+GGG	-	chr7.4:76597386-76597405	None:intergenic	40.0%
!	GCAGATATGGGTTATGCTAT+TGG	+	chr7.4:76597468-76597487	MS.gene74084:CDS	40.0%
!!	AGAGGGTATTGGAAAAGCTA+GGG	+	chr7.4:76597616-76597635	MS.gene74084:CDS	40.0%
	ACCACTCCATTGGAATGGTT+TGG	+	chr7.4:76597412-76597431	MS.gene74084:CDS	45.0%
	CTTGCAACCTTCTCAAATCG+GGG	-	chr7.4:76597566-76597585	None:intergenic	45.0%
	GAGTAACTGAGCATACTCGA+TGG	+	chr7.4:76597304-76597323	MS.gene74084:CDS	45.0%
	GCTTGCAACCTTCTCAAATC+GGG	-	chr7.4:76597567-76597586	None:intergenic	45.0%
	GGATTCAACTATTGGGGTCA+AGG	+	chr7.4:76597489-76597508	MS.gene74084:CDS	45.0%
	TCCAAACCATTCCAATGGAG+TGG	-	chr7.4:76597416-76597435	None:intergenic	45.0%
	TGATGACCACTCCATTGGAA+TGG	+	chr7.4:76597407-76597426	MS.gene74084:CDS	45.0%
	TTCTCAAATCGGGGAACTCA+TGG	-	chr7.4:76597557-76597576	None:intergenic	45.0%
!	ATGAGTTCCCCGATTTGAGA+AGG	+	chr7.4:76597556-76597575	MS.gene74084:CDS	45.0%
!	CAAAGAGGGTTTGCTCAGAA+AGG	+	chr7.4:76597644-76597663	MS.gene74084:CDS	45.0%
!	CATAGCTTCTCAGAGGGTAT+TGG	+	chr7.4:76597605-76597624	MS.gene74084:CDS	45.0%
!	TGTCTTGATGACCACTCCAT+TGG	+	chr7.4:76597402-76597421	MS.gene74084:CDS	45.0%
!!	CAGAGGGTATTGGAAAAGCT+AGG	+	chr7.4:76597615-76597634	MS.gene74084:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	76597300	76597746	76597300	ID=MS.gene74084
chr7.4	mRNA	76597300	76597746	76597300	ID=MS.gene74084.t1;Parent=MS.gene74084
chr7.4	exon	76597300	76597746	76597300	ID=MS.gene74084.t1.exon1;Parent=MS.gene74084.t1
chr7.4	CDS	76597300	76597746	76597300	ID=cds.MS.gene74084.t1;Parent=MS.gene74084.t1

Gene Sequence

>MS.gene74084

ATGAGAGTAACTGAGCATACTCGATGGGAAACTTGTAATTCAAAGAAAGAAGCTTTGGATTTCATAAAAAATGAATGCATTTACCCCTTACGCCAATCAATATGTCTTGATGACCACTCCATTGGAATGGTTTGGATTCTTCCTCATGCTAATGATGAGAAGTATAAAGCAGATATGGGTTATGCTATTGGATTCAACTATTGGGGTCAAGGAATTGCCACTAATGCAGTGAAGATTCTTCTGTCAAAAGTGTTCCATGAGTTCCCCGATTTGAGAAGGTTGCAAGCTTATACTGTTCTAGAGAACATAGCTTCTCAGAGGGTATTGGAAAAGCTAGGGTTCTACAAAGAGGGTTTGCTCAGAAAGGTTTTTTATTTTAAAGGAAACTTTGTGGATTTCTATATTTTTAGCTTTCTTTGGACAGATGAAATTCCTTCTGTTGTTTAG

Protein sequence

>MS.gene74084.t1

MRVTEHTRWETCNSKKEALDFIKNECIYPLRQSICLDDHSIGMVWILPHANDEKYKADMGYAIGFNYWGQGIATNAVKILLSKVFHEFPDLRRLQAYTVLENIASQRVLEKLGFYKEGLLRKVFYFKGNFVDFYIFSFLWTDEIPSVV