Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76226.t1 | XP_013470277.1 | 89.6 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.90E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76226.t1 | A0A072W228 | 89.6 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.3e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene76226 | 0.807058 | 6.08E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene76226 | 0.822277 | 2.58E-53 | -1.69E-46 |
| MS.gene05288 | MS.gene76226 | 0.825011 | 5.91E-54 | -1.69E-46 |
| MS.gene05289 | MS.gene76226 | 0.811812 | 5.81E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene76226 | 0.825139 | 5.52E-54 | -1.69E-46 |
| MS.gene053184 | MS.gene76226 | 0.804334 | 2.27E-49 | -1.69E-46 |
| MS.gene054184 | MS.gene76226 | 0.810473 | 1.13E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene76226 | 0.81309 | 3.05E-51 | -1.69E-46 |
| MS.gene054713 | MS.gene76226 | 0.882577 | 9.27E-71 | -1.69E-46 |
| MS.gene055356 | MS.gene76226 | 0.811409 | 7.10E-51 | -1.69E-46 |
| MS.gene055357 | MS.gene76226 | 0.808536 | 2.95E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene76226 | 0.815659 | 8.26E-52 | -1.69E-46 |
| MS.gene055608 | MS.gene76226 | 0.843293 | 1.53E-58 | -1.69E-46 |
| MS.gene055860 | MS.gene76226 | 0.809879 | 1.52E-50 | -1.69E-46 |
| MS.gene058042 | MS.gene76226 | 0.860137 | 2.55E-63 | -1.69E-46 |
| MS.gene058168 | MS.gene76226 | 0.829762 | 4.29E-55 | -1.69E-46 |
| MS.gene059609 | MS.gene76226 | 0.804836 | 1.78E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene76226 | -0.818379 | 2.02E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76226.t1 | MTR_1g112150 | 89.565 | 115 | 11 | 1 | 1 | 115 | 183 | 296 | 1.50e-71 | 215 |
| MS.gene76226.t1 | MTR_1g112160 | 64.706 | 119 | 41 | 1 | 1 | 119 | 134 | 251 | 7.03e-49 | 156 |
| MS.gene76226.t1 | MTR_2g021690 | 47.312 | 93 | 48 | 1 | 3 | 94 | 188 | 280 | 4.06e-20 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76226.t1 | AT1G55240 | 42.000 | 100 | 58 | 0 | 3 | 102 | 190 | 289 | 2.06e-25 | 97.4 |
| MS.gene76226.t1 | AT1G55230 | 41.905 | 105 | 60 | 1 | 1 | 105 | 188 | 291 | 4.67e-25 | 96.3 |
| MS.gene76226.t1 | AT5G19870 | 47.727 | 88 | 46 | 0 | 3 | 90 | 189 | 276 | 3.57e-24 | 93.6 |
Find 24 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATTATGGCTATAATTTA+TGG | 0.111898 | 1.1:+79227915 | MS.gene76226:CDS |
| CAATTCAGCTGGTTCTTTAT+TGG | 0.239215 | 1.1:+79227864 | MS.gene76226:CDS |
| GGTTTAGTGCTTTCTATATC+TGG | 0.242800 | 1.1:+79227738 | MS.gene76226:CDS |
| TCAACCCAAAGGTTGCTTCT+TGG | 0.301483 | 1.1:+79227764 | MS.gene76226:CDS |
| AGGTTTGTTCATTATGTTCA+TGG | 0.326708 | 1.1:+79227716 | MS.gene76226:CDS |
| AGTGTCTTTCTACTTGATTA+TGG | 0.354291 | 1.1:+79227902 | MS.gene76226:CDS |
| ATATTCCAAGAAGCAACCTT+TGG | 0.390404 | 1.1:-79227769 | None:intergenic |
| AAGGAGATCAACTTATGATT+AGG | 0.413259 | 1.1:+79227793 | MS.gene76226:CDS |
| CCAGATGATTTCTACTAGAA+TGG | 0.432826 | 1.1:+79228028 | MS.gene76226:CDS |
| TGGTGAATCTTCAATTCAGC+TGG | 0.442803 | 1.1:+79227853 | MS.gene76226:CDS |
| CCATTCTAGTAGAAATCATC+TGG | 0.450188 | 1.1:-79228028 | None:intergenic |
| GGTTTGTTCATTATGTTCAT+GGG | 0.458171 | 1.1:+79227717 | MS.gene76226:CDS |
| TATTCCAAGAAGCAACCTTT+GGG | 0.461299 | 1.1:-79227768 | None:intergenic |
| TATGCATCGTGCTGTTTCAT+TGG | 0.468772 | 1.1:+79227833 | MS.gene76226:CDS |
| GATTAGGTGCAATGATGAAA+AGG | 0.487514 | 1.1:+79227809 | MS.gene76226:CDS |
| GGTTGCTTCTTGGAATATGA+AGG | 0.488311 | 1.1:+79227774 | MS.gene76226:CDS |
| TGAACATAATGAACAAACCT+TGG | 0.523714 | 1.1:-79227713 | None:intergenic |
| TCTGTTAGCATGATCTTCCA+AGG | 0.532759 | 1.1:+79227696 | None:intergenic |
| GGAGTGCGATTCACTGATGA+AGG | 0.536386 | 1.1:+79227947 | MS.gene76226:CDS |
| AAGGAGGAACAGTACGGTGA+AGG | 0.576386 | 1.1:+79227966 | MS.gene76226:CDS |
| GAACAGTACGGTGAAGGCGA+TGG | 0.598192 | 1.1:+79227972 | MS.gene76226:CDS |
| ATATCTGGTTATCAACCCAA+AGG | 0.602519 | 1.1:+79227753 | MS.gene76226:CDS |
| GTGCGATTCACTGATGAAGG+AGG | 0.666959 | 1.1:+79227950 | MS.gene76226:CDS |
| CTGATGAAGGAGGAACAGTA+CGG | 0.675878 | 1.1:+79227960 | MS.gene76226:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATTTATGGTGAAAAAG+TGG | + | chr1.1:79227926-79227945 | MS.gene76226:CDS | 20.0% |
| !! | TTGATTATGGCTATAATTTA+TGG | + | chr1.1:79227915-79227934 | MS.gene76226:CDS | 20.0% |
| AAGGAGATCAACTTATGATT+AGG | + | chr1.1:79227793-79227812 | MS.gene76226:CDS | 30.0% | |
| AGTGTCTTTCTACTTGATTA+TGG | + | chr1.1:79227902-79227921 | MS.gene76226:CDS | 30.0% | |
| TGAACATAATGAACAAACCT+TGG | - | chr1.1:79227716-79227735 | None:intergenic | 30.0% | |
| ! | AGGTTTGTTCATTATGTTCA+TGG | + | chr1.1:79227716-79227735 | MS.gene76226:CDS | 30.0% |
| ! | GGTTTGTTCATTATGTTCAT+GGG | + | chr1.1:79227717-79227736 | MS.gene76226:CDS | 30.0% |
| ATATCTGGTTATCAACCCAA+AGG | + | chr1.1:79227753-79227772 | MS.gene76226:CDS | 35.0% | |
| ATATTCCAAGAAGCAACCTT+TGG | - | chr1.1:79227772-79227791 | None:intergenic | 35.0% | |
| CCAGATGATTTCTACTAGAA+TGG | + | chr1.1:79228028-79228047 | MS.gene76226:CDS | 35.0% | |
| CCATTCTAGTAGAAATCATC+TGG | - | chr1.1:79228031-79228050 | None:intergenic | 35.0% | |
| GATTAGGTGCAATGATGAAA+AGG | + | chr1.1:79227809-79227828 | MS.gene76226:CDS | 35.0% | |
| GGTTTAGTGCTTTCTATATC+TGG | + | chr1.1:79227738-79227757 | MS.gene76226:CDS | 35.0% | |
| TATTCCAAGAAGCAACCTTT+GGG | - | chr1.1:79227771-79227790 | None:intergenic | 35.0% | |
| ! | CAATTCAGCTGGTTCTTTAT+TGG | + | chr1.1:79227864-79227883 | MS.gene76226:CDS | 35.0% |
| GGTTGCTTCTTGGAATATGA+AGG | + | chr1.1:79227774-79227793 | MS.gene76226:CDS | 40.0% | |
| TGGTGAATCTTCAATTCAGC+TGG | + | chr1.1:79227853-79227872 | MS.gene76226:CDS | 40.0% | |
| !! | TATGCATCGTGCTGTTTCAT+TGG | + | chr1.1:79227833-79227852 | MS.gene76226:CDS | 40.0% |
| CTGATGAAGGAGGAACAGTA+CGG | + | chr1.1:79227960-79227979 | MS.gene76226:CDS | 45.0% | |
| TCAACCCAAAGGTTGCTTCT+TGG | + | chr1.1:79227764-79227783 | MS.gene76226:CDS | 45.0% | |
| AAGGAGGAACAGTACGGTGA+AGG | + | chr1.1:79227966-79227985 | MS.gene76226:CDS | 50.0% | |
| GGAGTGCGATTCACTGATGA+AGG | + | chr1.1:79227947-79227966 | MS.gene76226:CDS | 50.0% | |
| GTGCGATTCACTGATGAAGG+AGG | + | chr1.1:79227950-79227969 | MS.gene76226:CDS | 50.0% | |
| GAACAGTACGGTGAAGGCGA+TGG | + | chr1.1:79227972-79227991 | MS.gene76226:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 79227705 | 79228064 | 79227705 | ID=MS.gene76226 |
| chr1.1 | mRNA | 79227705 | 79228064 | 79227705 | ID=MS.gene76226.t1;Parent=MS.gene76226 |
| chr1.1 | exon | 79227705 | 79228064 | 79227705 | ID=MS.gene76226.t1.exon1;Parent=MS.gene76226.t1 |
| chr1.1 | CDS | 79227705 | 79228064 | 79227705 | ID=cds.MS.gene76226.t1;Parent=MS.gene76226.t1 |
| Gene Sequence |
| Protein sequence |
