Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76916.t1 | RHN44081.1 | 90.3 | 93 | 9 | 0 | 1 | 93 | 5 | 97 | 1.10E-34 | 155.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76916.t1 | A0A072TWH2 | 90.3 | 93 | 9 | 0 | 1 | 93 | 18 | 110 | 8.1e-35 | 155.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene76916 | 0.811976 | 5.35E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene76916 | 0.80986 | 1.54E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene76916 | 0.805906 | 1.06E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene76916 | 0.800171 | 1.63E-48 | -1.69E-46 |
| MS.gene050119 | MS.gene76916 | 0.821507 | 3.89E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene76916 | 0.809052 | 2.29E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene76916 | 0.828652 | 7.98E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene76916 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene76916 | 0.806143 | 9.49E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene76916 | 0.803659 | 3.14E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene76916 | 0.801043 | 1.08E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene76916 | 0.812086 | 5.06E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene76916 | 0.807479 | 4.95E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene76916 | 0.809466 | 1.87E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene76916 | 0.806255 | 8.99E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene76916 | 0.815798 | 7.69E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene76916 | 0.811401 | 7.13E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene76916 | 0.825285 | 5.10E-54 | -1.69E-46 |
| MS.gene053998 | MS.gene76916 | 0.813079 | 3.07E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene76916 | 0.810794 | 9.65E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene76916 | 0.806029 | 1.00E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene76916 | 0.819725 | 9.99E-53 | -1.69E-46 |
| MS.gene055808 | MS.gene76916 | 0.809819 | 1.57E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene76916 | 0.843577 | 1.29E-58 | -1.69E-46 |
| MS.gene056162 | MS.gene76916 | 0.801761 | 7.73E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene76916 | 0.816847 | 4.48E-52 | -1.69E-46 |
| MS.gene056498 | MS.gene76916 | 0.816016 | 6.87E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene76916 | 0.832346 | 9.96E-56 | -1.69E-46 |
| MS.gene057314 | MS.gene76916 | 0.806116 | 9.62E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene76916 | 0.807665 | 4.52E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene76916 | 0.851256 | 9.99E-61 | -1.69E-46 |
| MS.gene058037 | MS.gene76916 | 0.808397 | 3.16E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene76916 | 0.82023 | 7.66E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene76916 | 0.816383 | 5.69E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene76916 | 0.815892 | 7.32E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene76916 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene060521 | MS.gene76916 | 0.818025 | 2.43E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76916.t1 | MTR_7g007290 | 90.323 | 93 | 9 | 0 | 1 | 93 | 18 | 110 | 9.73e-57 | 170 |
| MS.gene76916.t1 | MTR_7g007300 | 80.000 | 70 | 14 | 0 | 1 | 70 | 1 | 70 | 2.48e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76916.t1 | AT1G47820 | 54.286 | 70 | 31 | 1 | 1 | 69 | 1 | 70 | 4.53e-20 | 77.4 |
| MS.gene76916.t1 | AT1G47820 | 54.286 | 70 | 31 | 1 | 1 | 69 | 1 | 70 | 4.53e-20 | 77.4 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGAGTCATATTCTGTTC+AGG | 0.414511 | 7.2:+91180431 | MS.gene76916:CDS |
| GTGGCATTCACCTGAGGTCT+AGG | 0.432602 | 7.2:-91180396 | None:intergenic |
| ACAAAGCATGGCCGTATTGA+AGG | 0.443308 | 7.2:+91180500 | MS.gene76916:CDS |
| TGTTATGAAATCCTTCAATA+CGG | 0.467267 | 7.2:-91180511 | None:intergenic |
| TATTCTGTTCAGGCAATCAA+TGG | 0.473465 | 7.2:+91180441 | MS.gene76916:CDS |
| TGACGATGATGTTGCTAAGA+AGG | 0.498240 | 7.2:+91180590 | MS.gene76916:CDS |
| TGCAGTGCTTCCTAGACCTC+AGG | 0.506425 | 7.2:+91180386 | MS.gene76916:CDS |
| TGCTAAGAAGGCTCAAGTAA+AGG | 0.526582 | 7.2:+91180602 | MS.gene76916:CDS |
| GAAGCTTCTAACGACTCGTC+TGG | 0.528210 | 7.2:-91180558 | None:intergenic |
| CGAGTCGTTAGAAGCTTCCT+CGG | 0.555746 | 7.2:+91180563 | MS.gene76916:CDS |
| GACTCGTCTGGAACATCTTG+AGG | 0.569302 | 7.2:-91180546 | None:intergenic |
| TAACATGAAAGAACAAAGCA+TGG | 0.616394 | 7.2:+91180488 | MS.gene76916:CDS |
| AACATCATCGTCATCTTCCG+AGG | 0.626307 | 7.2:-91180580 | None:intergenic |
| CGAAATGTGGCATTCACCTG+AGG | 0.643629 | 7.2:-91180402 | None:intergenic |
| CTCTGTGCTGAGACGAAATG+TGG | 0.644541 | 7.2:-91180415 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTTATGAAATCCTTCAATA+CGG | - | chr7.2:91180514-91180533 | None:intergenic | 25.0% |
| TAACATGAAAGAACAAAGCA+TGG | + | chr7.2:91180488-91180507 | MS.gene76916:CDS | 30.0% | |
| TATTCTGTTCAGGCAATCAA+TGG | + | chr7.2:91180441-91180460 | MS.gene76916:CDS | 35.0% | |
| CACAGAGTCATATTCTGTTC+AGG | + | chr7.2:91180431-91180450 | MS.gene76916:CDS | 40.0% | |
| TGACGATGATGTTGCTAAGA+AGG | + | chr7.2:91180590-91180609 | MS.gene76916:CDS | 40.0% | |
| TGCTAAGAAGGCTCAAGTAA+AGG | + | chr7.2:91180602-91180621 | MS.gene76916:CDS | 40.0% | |
| AACATCATCGTCATCTTCCG+AGG | - | chr7.2:91180583-91180602 | None:intergenic | 45.0% | |
| ACAAAGCATGGCCGTATTGA+AGG | + | chr7.2:91180500-91180519 | MS.gene76916:CDS | 45.0% | |
| CGAAATGTGGCATTCACCTG+AGG | - | chr7.2:91180405-91180424 | None:intergenic | 50.0% | |
| CGAGTCGTTAGAAGCTTCCT+CGG | + | chr7.2:91180563-91180582 | MS.gene76916:CDS | 50.0% | |
| CTCTGTGCTGAGACGAAATG+TGG | - | chr7.2:91180418-91180437 | None:intergenic | 50.0% | |
| GAAGCTTCTAACGACTCGTC+TGG | - | chr7.2:91180561-91180580 | None:intergenic | 50.0% | |
| ! | GACTCGTCTGGAACATCTTG+AGG | - | chr7.2:91180549-91180568 | None:intergenic | 50.0% |
| GTGGCATTCACCTGAGGTCT+AGG | - | chr7.2:91180399-91180418 | None:intergenic | 55.0% | |
| TGCAGTGCTTCCTAGACCTC+AGG | + | chr7.2:91180386-91180405 | MS.gene76916:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 91180366 | 91180647 | 91180366 | ID=MS.gene76916 |
| chr7.2 | mRNA | 91180366 | 91180647 | 91180366 | ID=MS.gene76916.t1;Parent=MS.gene76916 |
| chr7.2 | exon | 91180366 | 91180647 | 91180366 | ID=MS.gene76916.t1.exon1;Parent=MS.gene76916.t1 |
| chr7.2 | CDS | 91180366 | 91180647 | 91180366 | ID=cds.MS.gene76916.t1;Parent=MS.gene76916.t1 |
| Gene Sequence |
| Protein sequence |
