Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77461.t1 | PNX74769.1 | 57.3 | 185 | 61 | 2 | 6 | 172 | 10 | 194 | 4.00E-46 | 194.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77461.t1 | A0A2K3L8B1 | 57.3 | 185 | 61 | 2 | 6 | 172 | 10 | 194 | 2.9e-46 | 194.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77461 | MS.gene77464 | PPI |
| MS.gene77461 | MS.gene044326 | PPI |
| MS.gene77472 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene70427 | PPI |
| MS.gene77461 | MS.gene70476 | PPI |
| MS.gene77461 | MS.gene049263 | PPI |
| MS.gene77461 | MS.gene25704 | PPI |
| MS.gene77461 | MS.gene77468 | PPI |
| MS.gene77461 | MS.gene71531 | PPI |
| MS.gene79115 | MS.gene77461 | PPI |
| MS.gene044370 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene044323 | PPI |
| MS.gene77461 | MS.gene71284 | PPI |
| MS.gene77461 | MS.gene044376 | PPI |
| MS.gene70443 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene045214 | PPI |
| MS.gene77461 | MS.gene77466 | PPI |
| MS.gene044363 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene044361 | PPI |
| MS.gene70430 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene71282 | PPI |
| MS.gene77461 | MS.gene71524 | PPI |
| MS.gene77461 | MS.gene71528 | PPI |
| MS.gene70418 | MS.gene77461 | PPI |
| MS.gene77461 | MS.gene70424 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77461.t1 | MTR_3g083080 | 51.892 | 185 | 75 | 1 | 2 | 172 | 23 | 207 | 8.22e-60 | 185 |
| MS.gene77461.t1 | MTR_3g083030 | 51.596 | 188 | 72 | 4 | 2 | 172 | 46 | 231 | 1.94e-55 | 174 |
| MS.gene77461.t1 | MTR_3g083050 | 53.846 | 169 | 61 | 3 | 19 | 172 | 42 | 208 | 4.14e-53 | 168 |
| MS.gene77461.t1 | MTR_3g086030 | 41.333 | 150 | 81 | 4 | 9 | 154 | 5 | 151 | 7.08e-27 | 99.4 |
| MS.gene77461.t1 | MTR_3g082420 | 31.579 | 209 | 94 | 5 | 7 | 170 | 17 | 221 | 2.01e-26 | 100 |
| MS.gene77461.t1 | MTR_3g082340 | 34.756 | 164 | 80 | 4 | 30 | 170 | 63 | 222 | 6.23e-24 | 94.0 |
| MS.gene77461.t1 | MTR_3g080680 | 32.632 | 190 | 95 | 7 | 9 | 170 | 14 | 198 | 2.05e-20 | 84.0 |
| MS.gene77461.t1 | MTR_3g083150 | 35.602 | 191 | 92 | 5 | 10 | 170 | 22 | 211 | 8.23e-17 | 74.7 |
| MS.gene77461.t1 | MTR_8g461300 | 31.937 | 191 | 91 | 10 | 12 | 170 | 15 | 198 | 9.64e-12 | 61.2 |
| MS.gene77461.t1 | MTR_3g083100 | 38.400 | 125 | 47 | 4 | 76 | 170 | 49 | 173 | 9.94e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTCTCCGGTTCCGATTT+TGG | 0.147266 | 3.2:-71873040 | None:intergenic |
| TCCCCTCTGCAAGCTTGCTT+TGG | 0.301525 | 3.2:+71873439 | MS.gene77461:CDS |
| GATACGCAACGAAGAACTTA+TGG | 0.347208 | 3.2:+71873072 | MS.gene77461:CDS |
| CCACATAAACACGCAATTGC+TGG | 0.351740 | 3.2:-71873368 | None:intergenic |
| CGCAATTGCTGGAGGGGAAA+TGG | 0.355122 | 3.2:-71873357 | None:intergenic |
| TGCGAACACATAAACATATA+AGG | 0.360061 | 3.2:-71873130 | None:intergenic |
| AGATGCTTACAGTTAGATTA+CGG | 0.371835 | 3.2:-71873231 | None:intergenic |
| CATGAGCTAGGTAGGCTTCA+TGG | 0.407712 | 3.2:-71873771 | None:intergenic |
| ATCGATTCCCACGGCCTCTC+CGG | 0.413278 | 3.2:-71873052 | None:intergenic |
| ACCGACGATGGAGCCAAAAT+CGG | 0.429034 | 3.2:+71873027 | MS.gene77461:CDS |
| AGACATGATCACAGGCGGTT+TGG | 0.436424 | 3.2:-71873744 | None:intergenic |
| CCAGCAATTGCGTGTTTATG+TGG | 0.442705 | 3.2:+71873368 | MS.gene77461:CDS |
| GATGCTTACAGTTAGATTAC+GGG | 0.444024 | 3.2:-71873230 | None:intergenic |
| AATCGGAACCGGAGAGGCCG+TGG | 0.444560 | 3.2:+71873044 | MS.gene77461:CDS |
| TGTGTTCGCACTTTGTGTAC+AGG | 0.475695 | 3.2:+71873143 | MS.gene77461:CDS |
| TGCAGAGGGGAGTGAGTTCA+AGG | 0.476525 | 3.2:-71873427 | None:intergenic |
| TGGCTCCATCGTCGGTGGTA+AGG | 0.478145 | 3.2:-71873020 | None:intergenic |
| AGGTCATCAGTCATGCGATC+AGG | 0.487173 | 3.2:-71873401 | None:intergenic |
| AGCAGGGCAGTTGGAAGGAT+GGG | 0.498741 | 3.2:-71873687 | None:intergenic |
| GATGGAGCCAAAATCGGAAC+CGG | 0.514435 | 3.2:+71873033 | MS.gene77461:CDS |
| TCGGCCTGAAAAGTTATAGC+AGG | 0.516661 | 3.2:-71873704 | None:intergenic |
| GAAATACAATGCTGAGAACC+AGG | 0.518077 | 3.2:+71873463 | MS.gene77461:CDS |
| CTCCAAAGCAAGCTTGCAGA+GGG | 0.518878 | 3.2:-71873441 | None:intergenic |
| TAGCAGGGCAGTTGGAAGGA+TGG | 0.540470 | 3.2:-71873688 | None:intergenic |
| AAAAGTTATAGCAGGGCAGT+TGG | 0.545343 | 3.2:-71873696 | None:intergenic |
| TATTAATGCATGAGCTAGGT+AGG | 0.547710 | 3.2:-71873779 | None:intergenic |
| AATAAGTCTTGAAACCTGCC+TGG | 0.551794 | 3.2:-71873481 | None:intergenic |
| TACAATGCTGAGAACCAGGC+AGG | 0.563638 | 3.2:+71873467 | MS.gene77461:CDS |
| GGACCTACTGCAAAAGACTT+CGG | 0.565394 | 3.2:-71873723 | None:intergenic |
| TAAACACGCAATTGCTGGAG+GGG | 0.569083 | 3.2:-71873363 | None:intergenic |
| GGGGAGTGAGTTCAAGGCGA+AGG | 0.569994 | 3.2:-71873421 | None:intergenic |
| GTTATAGCAGGGCAGTTGGA+AGG | 0.582712 | 3.2:-71873692 | None:intergenic |
| GCACTTTGTGTACAGGAACA+AGG | 0.588895 | 3.2:+71873150 | MS.gene77461:CDS |
| GCAATTGCGTGTTTATGTGG+CGG | 0.605533 | 3.2:+71873371 | MS.gene77461:CDS |
| TCGTTGCGTATCGATTCCCA+CGG | 0.608411 | 3.2:-71873061 | None:intergenic |
| TCTCCAAAGCAAGCTTGCAG+AGG | 0.618622 | 3.2:-71873442 | None:intergenic |
| TGAAGAAATTAGTGTCGCCA+TGG | 0.621839 | 3.2:+71873189 | MS.gene77461:CDS |
| AGCTACCTTACCACCGACGA+TGG | 0.624415 | 3.2:+71873015 | MS.gene77461:CDS |
| CATAAACACGCAATTGCTGG+AGG | 0.627436 | 3.2:-71873365 | None:intergenic |
| CGGCCTGAAAAGTTATAGCA+GGG | 0.628675 | 3.2:-71873703 | None:intergenic |
| ATCGGAACCGGAGAGGCCGT+GGG | 0.634575 | 3.2:+71873045 | MS.gene77461:CDS |
| ATGGCACAAGACATGATCAC+AGG | 0.636485 | 3.2:-71873752 | None:intergenic |
| ATAAACACGCAATTGCTGGA+GGG | 0.642196 | 3.2:-71873364 | None:intergenic |
| AGCCAAAATCGGAACCGGAG+AGG | 0.658574 | 3.2:+71873038 | MS.gene77461:CDS |
| GCACAAGACATGATCACAGG+CGG | 0.708906 | 3.2:-71873749 | None:intergenic |
| TCCAAAGCAAGCTTGCAGAG+GGG | 0.721977 | 3.2:-71873440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTATTCTTGTTAATTATTA+GGG | + | chr3.2:71873578-71873597 | MS.gene77461:intron | 15.0% |
| !! | TACTATTCTTGTTAATTATT+AGG | + | chr3.2:71873577-71873596 | MS.gene77461:intron | 15.0% |
| !!! | TAATCTCTTAATTTTGATTC+TGG | + | chr3.2:71873320-71873339 | MS.gene77461:intron | 20.0% |
| ! | AATTAAGTGAAACGCAAAAT+TGG | - | chr3.2:71873280-71873299 | None:intergenic | 25.0% |
| AATACACAATATTACCTCCA+TGG | - | chr3.2:71873640-71873659 | None:intergenic | 30.0% | |
| AGATGCTTACAGTTAGATTA+CGG | - | chr3.2:71873234-71873253 | None:intergenic | 30.0% | |
| ATACACAATATTACCTCCAT+GGG | - | chr3.2:71873639-71873658 | None:intergenic | 30.0% | |
| TGCGAACACATAAACATATA+AGG | - | chr3.2:71873133-71873152 | None:intergenic | 30.0% | |
| !!! | TCTTAATTTTGATTCTGGAC+AGG | + | chr3.2:71873325-71873344 | MS.gene77461:intron | 30.0% |
| GATGCTTACAGTTAGATTAC+GGG | - | chr3.2:71873233-71873252 | None:intergenic | 35.0% | |
| AAAAGTTATAGCAGGGCAGT+TGG | - | chr3.2:71873699-71873718 | None:intergenic | 40.0% | |
| AACATTGTCAAGTCAACCCA+TGG | + | chr3.2:71873620-71873639 | MS.gene77461:intron | 40.0% | |
| AATAAGTCTTGAAACCTGCC+TGG | - | chr3.2:71873484-71873503 | None:intergenic | 40.0% | |
| ATAAACACGCAATTGCTGGA+GGG | - | chr3.2:71873367-71873386 | None:intergenic | 40.0% | |
| GAAATACAATGCTGAGAACC+AGG | + | chr3.2:71873463-71873482 | MS.gene77461:CDS | 40.0% | |
| GATACGCAACGAAGAACTTA+TGG | + | chr3.2:71873072-71873091 | MS.gene77461:CDS | 40.0% | |
| TGAAGAAATTAGTGTCGCCA+TGG | + | chr3.2:71873189-71873208 | MS.gene77461:CDS | 40.0% | |
| ATGGCACAAGACATGATCAC+AGG | - | chr3.2:71873755-71873774 | None:intergenic | 45.0% | |
| ATTGTCAAGTCAACCCATGG+AGG | + | chr3.2:71873623-71873642 | MS.gene77461:intron | 45.0% | |
| CATAAACACGCAATTGCTGG+AGG | - | chr3.2:71873368-71873387 | None:intergenic | 45.0% | |
| CCACATAAACACGCAATTGC+TGG | - | chr3.2:71873371-71873390 | None:intergenic | 45.0% | |
| CCAGCAATTGCGTGTTTATG+TGG | + | chr3.2:71873368-71873387 | MS.gene77461:CDS | 45.0% | |
| CGGCCTGAAAAGTTATAGCA+GGG | - | chr3.2:71873706-71873725 | None:intergenic | 45.0% | |
| GCAATTGCGTGTTTATGTGG+CGG | + | chr3.2:71873371-71873390 | MS.gene77461:CDS | 45.0% | |
| GCACTTTGTGTACAGGAACA+AGG | + | chr3.2:71873150-71873169 | MS.gene77461:CDS | 45.0% | |
| GGACCTACTGCAAAAGACTT+CGG | - | chr3.2:71873726-71873745 | None:intergenic | 45.0% | |
| TAAACACGCAATTGCTGGAG+GGG | - | chr3.2:71873366-71873385 | None:intergenic | 45.0% | |
| TCGGCCTGAAAAGTTATAGC+AGG | - | chr3.2:71873707-71873726 | None:intergenic | 45.0% | |
| TGTGTTCGCACTTTGTGTAC+AGG | + | chr3.2:71873143-71873162 | MS.gene77461:CDS | 45.0% | |
| ! | CTGCCCTGCTATAACTTTTC+AGG | + | chr3.2:71873700-71873719 | MS.gene77461:CDS | 45.0% |
| ! | GGCTGCATCTTTTGCTTGAA+AGG | - | chr3.2:71873669-71873688 | None:intergenic | 45.0% |
| ACCGACGATGGAGCCAAAAT+CGG | + | chr3.2:71873027-71873046 | MS.gene77461:CDS | 50.0% | |
| AGACATGATCACAGGCGGTT+TGG | - | chr3.2:71873747-71873766 | None:intergenic | 50.0% | |
| AGGTCATCAGTCATGCGATC+AGG | - | chr3.2:71873404-71873423 | None:intergenic | 50.0% | |
| CTCCAAAGCAAGCTTGCAGA+GGG | - | chr3.2:71873444-71873463 | None:intergenic | 50.0% | |
| GATGGAGCCAAAATCGGAAC+CGG | + | chr3.2:71873033-71873052 | MS.gene77461:CDS | 50.0% | |
| GCACAAGACATGATCACAGG+CGG | - | chr3.2:71873752-71873771 | None:intergenic | 50.0% | |
| GTTATAGCAGGGCAGTTGGA+AGG | - | chr3.2:71873695-71873714 | None:intergenic | 50.0% | |
| TACAATGCTGAGAACCAGGC+AGG | + | chr3.2:71873467-71873486 | MS.gene77461:CDS | 50.0% | |
| TCCAAAGCAAGCTTGCAGAG+GGG | - | chr3.2:71873443-71873462 | None:intergenic | 50.0% | |
| TCTCCAAAGCAAGCTTGCAG+AGG | - | chr3.2:71873445-71873464 | None:intergenic | 50.0% | |
| ! | AGGCCGAAGTCTTTTGCAGT+AGG | + | chr3.2:71873720-71873739 | MS.gene77461:CDS | 50.0% |
| !! | GCGCTTTTTATGCTCAGCCA+TGG | - | chr3.2:71873209-71873228 | None:intergenic | 50.0% |
| !! | TCGTTGCGTATCGATTCCCA+CGG | - | chr3.2:71873064-71873083 | None:intergenic | 50.0% |
| !!! | TCCGATTTTGGCTCCATCGT+CGG | - | chr3.2:71873031-71873050 | None:intergenic | 50.0% |
| AGCAGGGCAGTTGGAAGGAT+GGG | - | chr3.2:71873690-71873709 | None:intergenic | 55.0% | |
| AGCCAAAATCGGAACCGGAG+AGG | + | chr3.2:71873038-71873057 | MS.gene77461:CDS | 55.0% | |
| AGCTACCTTACCACCGACGA+TGG | + | chr3.2:71873015-71873034 | MS.gene77461:CDS | 55.0% | |
| CGCAATTGCTGGAGGGGAAA+TGG | - | chr3.2:71873360-71873379 | None:intergenic | 55.0% | |
| TAGCAGGGCAGTTGGAAGGA+TGG | - | chr3.2:71873691-71873710 | None:intergenic | 55.0% | |
| TGCAGAGGGGAGTGAGTTCA+AGG | - | chr3.2:71873430-71873449 | None:intergenic | 55.0% | |
| ! | TCCCCTCTGCAAGCTTGCTT+TGG | + | chr3.2:71873439-71873458 | MS.gene77461:CDS | 55.0% |
| !!! | GATTTTGGCTCCATCGTCGG+TGG | - | chr3.2:71873028-71873047 | None:intergenic | 55.0% |
| GGGGAGTGAGTTCAAGGCGA+AGG | - | chr3.2:71873424-71873443 | None:intergenic | 60.0% | |
| TGGCTCCATCGTCGGTGGTA+AGG | - | chr3.2:71873023-71873042 | None:intergenic | 60.0% | |
| ! | ATCGATTCCCACGGCCTCTC+CGG | - | chr3.2:71873055-71873074 | None:intergenic | 60.0% |
| ! | GGCCTCTCCGGTTCCGATTT+TGG | - | chr3.2:71873043-71873062 | None:intergenic | 60.0% |
| AATCGGAACCGGAGAGGCCG+TGG | + | chr3.2:71873044-71873063 | MS.gene77461:CDS | 65.0% | |
| ATCGGAACCGGAGAGGCCGT+GGG | + | chr3.2:71873045-71873064 | MS.gene77461:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71873010 | 71873787 | 71873010 | ID=MS.gene77461 |
| chr3.2 | mRNA | 71873010 | 71873787 | 71873010 | ID=MS.gene77461.t1;Parent=MS.gene77461 |
| chr3.2 | exon | 71873010 | 71873243 | 71873010 | ID=MS.gene77461.t1.exon1;Parent=MS.gene77461.t1 |
| chr3.2 | CDS | 71873010 | 71873243 | 71873010 | ID=cds.MS.gene77461.t1;Parent=MS.gene77461.t1 |
| chr3.2 | exon | 71873347 | 71873488 | 71873347 | ID=MS.gene77461.t1.exon2;Parent=MS.gene77461.t1 |
| chr3.2 | CDS | 71873347 | 71873488 | 71873347 | ID=cds.MS.gene77461.t1;Parent=MS.gene77461.t1 |
| chr3.2 | exon | 71873645 | 71873787 | 71873645 | ID=MS.gene77461.t1.exon3;Parent=MS.gene77461.t1 |
| chr3.2 | CDS | 71873645 | 71873787 | 71873645 | ID=cds.MS.gene77461.t1;Parent=MS.gene77461.t1 |
| Gene Sequence |
| Protein sequence |
