Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77462.t1 | PNX57102.1 | 50.8 | 122 | 50 | 2 | 94 | 205 | 11 | 132 | 3.40E-23 | 118.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77462.t1 | A0A392P375 | 50.4 | 123 | 51 | 2 | 94 | 206 | 46 | 168 | 1.4e-23 | 119.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70443 | MS.gene77462 | PPI |
| MS.gene77462 | MS.gene044326 | PPI |
| MS.gene71282 | MS.gene77462 | PPI |
| MS.gene70424 | MS.gene77462 | PPI |
| MS.gene70427 | MS.gene77462 | PPI |
| MS.gene71528 | MS.gene77462 | PPI |
| MS.gene044376 | MS.gene77462 | PPI |
| MS.gene044363 | MS.gene77462 | PPI |
| MS.gene70430 | MS.gene77462 | PPI |
| MS.gene77464 | MS.gene77462 | PPI |
| MS.gene77462 | MS.gene049263 | PPI |
| MS.gene70418 | MS.gene77462 | PPI |
| MS.gene71284 | MS.gene77462 | PPI |
| MS.gene77462 | MS.gene044323 | PPI |
| MS.gene77472 | MS.gene77462 | PPI |
| MS.gene71531 | MS.gene77462 | PPI |
| MS.gene77466 | MS.gene77462 | PPI |
| MS.gene77468 | MS.gene77462 | PPI |
| MS.gene25704 | MS.gene77462 | PPI |
| MS.gene77462 | MS.gene71524 | PPI |
| MS.gene045214 | MS.gene77462 | PPI |
| MS.gene044361 | MS.gene77462 | PPI |
| MS.gene79115 | MS.gene77462 | PPI |
| MS.gene044370 | MS.gene77462 | PPI |
| MS.gene70476 | MS.gene77462 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77462.t1 | MTR_3g083080 | 34.158 | 202 | 84 | 7 | 51 | 207 | 10 | 207 | 1.02e-22 | 91.3 |
| MS.gene77462.t1 | MTR_3g083050 | 33.005 | 203 | 84 | 7 | 50 | 205 | 9 | 206 | 5.14e-19 | 81.6 |
| MS.gene77462.t1 | MTR_3g083030 | 31.278 | 227 | 81 | 8 | 49 | 205 | 8 | 229 | 1.09e-18 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 67 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCATCGGTTATGTAAA+TGG | 0.201913 | 3.2:-71906162 | None:intergenic |
| ACTCTTTCCTCGCAGCTTTC+CGG | 0.228598 | 3.2:-71905885 | None:intergenic |
| CTCTTTCCTCGCAGCTTTCC+GGG | 0.234421 | 3.2:-71905884 | None:intergenic |
| TCCTTCACCCAGTCCAGTTC+GGG | 0.311535 | 3.2:-71905945 | None:intergenic |
| TTCCAGACCGAAATCTTTGA+TGG | 0.325654 | 3.2:+71906405 | MS.gene77462:CDS |
| TTCCTTCACCCAGTCCAGTT+CGG | 0.326573 | 3.2:-71905946 | None:intergenic |
| TTCGGATTCGGAACCCGAAC+TGG | 0.342471 | 3.2:+71905932 | MS.gene77462:CDS |
| GAAGATTGGTGTATTCGTTT+AGG | 0.355836 | 3.2:+71905780 | MS.gene77462:CDS |
| CGTGCAAGCAGGCAGGTATC+CGG | 0.360773 | 3.2:-71905845 | None:intergenic |
| AGGAATTCCATCAAAGATTT+CGG | 0.365759 | 3.2:-71906412 | None:intergenic |
| TACCTTCTCCAATGCTGATC+AGG | 0.369494 | 3.2:+71906199 | MS.gene77462:CDS |
| GCAGCTTTCCGGGGAAGGTT+TGG | 0.373458 | 3.2:-71905874 | None:intergenic |
| TCCTCGCAGCTTTCCGGGGA+AGG | 0.382586 | 3.2:-71905879 | None:intergenic |
| AGGCAGGTATCCGGGGAATC+TGG | 0.397204 | 3.2:-71905836 | None:intergenic |
| CTGGGTGAAGGAAGAGGAAG+AGG | 0.399267 | 3.2:+71905956 | MS.gene77462:CDS |
| ATCAAAATCAGATTCGGATT+CGG | 0.425852 | 3.2:+71905920 | MS.gene77462:CDS |
| TTGTGTTTGATGACCTTGTC+AGG | 0.426633 | 3.2:+71906292 | MS.gene77462:CDS |
| ATTCGGAACCCGAACTGGAC+TGG | 0.431668 | 3.2:+71905937 | MS.gene77462:CDS |
| CCTTGGTGTATATGTTAGGC+AGG | 0.432340 | 3.2:-71906474 | None:intergenic |
| ACATCATCGGTTATGTAAAT+GGG | 0.460339 | 3.2:-71906161 | None:intergenic |
| AGAGGAAGAGGAAGAAAGAA+AGG | 0.461387 | 3.2:+71905968 | MS.gene77462:CDS |
| CGGATACCTGCCTGCTTGCA+CGG | 0.462149 | 3.2:+71905846 | MS.gene77462:CDS |
| GTGCAAGCAGGCAGGTATCC+GGG | 0.463198 | 3.2:-71905844 | None:intergenic |
| TAGCTTGAAAGGTAATGTAA+TGG | 0.463220 | 3.2:-71906344 | None:intergenic |
| TATATGTTAGGCAGGCTTCA+TGG | 0.472665 | 3.2:-71906466 | None:intergenic |
| GCTGAAATCAAAATCAGATT+CGG | 0.474119 | 3.2:+71905914 | MS.gene77462:CDS |
| TTTGATGGAATTCCTTCAGA+TGG | 0.478240 | 3.2:+71906420 | MS.gene77462:CDS |
| CGGCATCACCAAACCTTCCC+CGG | 0.481301 | 3.2:+71905866 | MS.gene77462:CDS |
| TCAACCCGTCAATGCGTTCA+AGG | 0.482744 | 3.2:+71906315 | MS.gene77462:CDS |
| TTCCATCAAAGATTTCGGTC+TGG | 0.484032 | 3.2:-71906407 | None:intergenic |
| TTTACTGATTCATAACCAAA+AGG | 0.490944 | 3.2:-71905723 | None:intergenic |
| AAAACCTTGAACGCATTGAC+GGG | 0.497313 | 3.2:-71906319 | None:intergenic |
| TGTAGCATCTTTAGCTTGAA+AGG | 0.500229 | 3.2:-71906355 | None:intergenic |
| TTGGTTATGAATCAGTAAAG+AGG | 0.501426 | 3.2:+71905727 | MS.gene77462:CDS |
| GAAAGTTATAGCAGGACTGC+TGG | 0.502772 | 3.2:-71906385 | None:intergenic |
| TTCGGAACCCGAACTGGACT+GGG | 0.514527 | 3.2:+71905938 | MS.gene77462:CDS |
| TAAAACCTTGAACGCATTGA+CGG | 0.514956 | 3.2:-71906320 | None:intergenic |
| GAGACGAGGGCGAACATCAT+CGG | 0.515251 | 3.2:-71906174 | None:intergenic |
| TTTGGTGATGCCGTGCAAGC+AGG | 0.518391 | 3.2:-71905856 | None:intergenic |
| ACTGGACTGGGTGAAGGAAG+AGG | 0.527129 | 3.2:+71905950 | MS.gene77462:CDS |
| AGAGGAAGAAAGAAAGGTGA+TGG | 0.530633 | 3.2:+71905974 | MS.gene77462:CDS |
| TATGAATCAGTAAAGAGGAT+TGG | 0.540096 | 3.2:+71905732 | MS.gene77462:CDS |
| TCGGTCTGGAAAGTTATAGC+AGG | 0.558638 | 3.2:-71906393 | None:intergenic |
| TTAGGCAGGCTTCATGGCAC+AGG | 0.558715 | 3.2:-71906460 | None:intergenic |
| GTGATGCCGTGCAAGCAGGC+AGG | 0.568682 | 3.2:-71905852 | None:intergenic |
| GTAATCAAAACCAGATTCCC+CGG | 0.571482 | 3.2:+71905826 | MS.gene77462:CDS |
| GACCCTCCACAAGGTTTATG+TGG | 0.573237 | 3.2:+71906128 | MS.gene77462:CDS |
| TCCTTCAGATGGACCGCCTG+TGG | 0.573790 | 3.2:+71906431 | MS.gene77462:CDS |
| ACCCGAACTGGACTGGGTGA+AGG | 0.577055 | 3.2:+71905944 | MS.gene77462:CDS |
| AACCTGATCAGCATTGGAGA+AGG | 0.579987 | 3.2:-71906201 | None:intergenic |
| TGCAAGCAGGCAGGTATCCG+GGG | 0.585236 | 3.2:-71905843 | None:intergenic |
| GACGCTATCGACCCTCCACA+AGG | 0.594289 | 3.2:+71906119 | MS.gene77462:CDS |
| ACCACAGGCGGTCCATCTGA+AGG | 0.603009 | 3.2:-71906432 | None:intergenic |
| TCTTTCCTCGCAGCTTTCCG+GGG | 0.603598 | 3.2:-71905883 | None:intergenic |
| ATGGCACAGGAACTAACCAC+AGG | 0.636401 | 3.2:-71906447 | None:intergenic |
| GTTATAGCAGGACTGCTGGA+AGG | 0.641064 | 3.2:-71906381 | None:intergenic |
| TGTATTCGTTTAGGTACATG+TGG | 0.642255 | 3.2:+71905789 | MS.gene77462:CDS |
| CGCCACATAAACCTTGTGGA+GGG | 0.644987 | 3.2:-71906130 | None:intergenic |
| CCGCCACATAAACCTTGTGG+AGG | 0.646649 | 3.2:-71906131 | None:intergenic |
| ATTGACGGGTTGACCTGACA+AGG | 0.647456 | 3.2:-71906305 | None:intergenic |
| ATACCGCCACATAAACCTTG+TGG | 0.663420 | 3.2:-71906134 | None:intergenic |
| TTGGAGAAGGTAATGAGACG+AGG | 0.668902 | 3.2:-71906188 | None:intergenic |
| TGGAGAAGGTAATGAGACGA+GGG | 0.670071 | 3.2:-71906187 | None:intergenic |
| CCTCCACAAGGTTTATGTGG+CGG | 0.672471 | 3.2:+71906131 | MS.gene77462:CDS |
| ACCTTCCCCGGAAAGCTGCG+AGG | 0.678912 | 3.2:+71905878 | MS.gene77462:CDS |
| GCACAGGAACTAACCACAGG+CGG | 0.694541 | 3.2:-71906444 | None:intergenic |
| TGATGGCTGTGTATAATGAG+CGG | 0.764880 | 3.2:+71905991 | MS.gene77462:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTCTTCTTAATTATT+AGG | + | chr3.2:71906260-71906279 | MS.gene77462:intron | 10.0% |
| !! | ATTATTCTTCTTAATTATTA+GGG | + | chr3.2:71906261-71906280 | MS.gene77462:intron | 10.0% |
| !! | AAGATAATAAGTGAAACAAA+GGG | - | chr3.2:71906072-71906091 | None:intergenic | 20.0% |
| !! | TAAGATAATAAGTGAAACAA+AGG | - | chr3.2:71906073-71906092 | None:intergenic | 20.0% |
| !! | TATATATACCAATAGTTTAC+TGG | - | chr3.2:71906232-71906251 | None:intergenic | 20.0% |
| !! | TTATTATCTTAATCTCATTG+TGG | + | chr3.2:71906082-71906101 | MS.gene77462:intron | 20.0% |
| ! | AACAATAGATAGAACATAGA+AGG | - | chr3.2:71906040-71906059 | None:intergenic | 25.0% |
| !!! | TTTACTGATTCATAACCAAA+AGG | - | chr3.2:71905726-71905745 | None:intergenic | 25.0% |
| AGGAATTCCATCAAAGATTT+CGG | - | chr3.2:71906415-71906434 | None:intergenic | 30.0% | |
| ATCAAAATCAGATTCGGATT+CGG | + | chr3.2:71905920-71905939 | MS.gene77462:CDS | 30.0% | |
| CTCGAAAAATAAACGAAGAT+TGG | + | chr3.2:71905766-71905785 | MS.gene77462:CDS | 30.0% | |
| GCTGAAATCAAAATCAGATT+CGG | + | chr3.2:71905914-71905933 | MS.gene77462:CDS | 30.0% | |
| GTTCAAAACCAGTAAACTAT+TGG | + | chr3.2:71906221-71906240 | MS.gene77462:intron | 30.0% | |
| TAGCTTGAAAGGTAATGTAA+TGG | - | chr3.2:71906347-71906366 | None:intergenic | 30.0% | |
| TATGAATCAGTAAAGAGGAT+TGG | + | chr3.2:71905732-71905751 | MS.gene77462:CDS | 30.0% | |
| TTGGTTATGAATCAGTAAAG+AGG | + | chr3.2:71905727-71905746 | MS.gene77462:CDS | 30.0% | |
| ! | AACATCATCGGTTATGTAAA+TGG | - | chr3.2:71906165-71906184 | None:intergenic | 30.0% |
| ! | ACATCATCGGTTATGTAAAT+GGG | - | chr3.2:71906164-71906183 | None:intergenic | 30.0% |
| !! | CAATCTTCGTTTATTTTTCG+AGG | - | chr3.2:71905768-71905787 | None:intergenic | 30.0% |
| GAAGATTGGTGTATTCGTTT+AGG | + | chr3.2:71905780-71905799 | MS.gene77462:CDS | 35.0% | |
| TAAAACCTTGAACGCATTGA+CGG | - | chr3.2:71906323-71906342 | None:intergenic | 35.0% | |
| TGTATTCGTTTAGGTACATG+TGG | + | chr3.2:71905789-71905808 | MS.gene77462:CDS | 35.0% | |
| TTTGATGGAATTCCTTCAGA+TGG | + | chr3.2:71906420-71906439 | MS.gene77462:CDS | 35.0% | |
| ! | AGTGAAACAAAGGGTACAAA+AGG | - | chr3.2:71906063-71906082 | None:intergenic | 35.0% |
| !! | TGTAGCATCTTTAGCTTGAA+AGG | - | chr3.2:71906358-71906377 | None:intergenic | 35.0% |
| AGAGGAAGAAAGAAAGGTGA+TGG | + | chr3.2:71905974-71905993 | MS.gene77462:CDS | 40.0% | |
| AGAGGAAGAGGAAGAAAGAA+AGG | + | chr3.2:71905968-71905987 | MS.gene77462:CDS | 40.0% | |
| GTAATCAAAACCAGATTCCC+CGG | + | chr3.2:71905826-71905845 | MS.gene77462:CDS | 40.0% | |
| TGATGGCTGTGTATAATGAG+CGG | + | chr3.2:71905991-71906010 | MS.gene77462:CDS | 40.0% | |
| TTCCAGACCGAAATCTTTGA+TGG | + | chr3.2:71906405-71906424 | MS.gene77462:CDS | 40.0% | |
| TTCCATCAAAGATTTCGGTC+TGG | - | chr3.2:71906410-71906429 | None:intergenic | 40.0% | |
| ! | AAAACCTTGAACGCATTGAC+GGG | - | chr3.2:71906322-71906341 | None:intergenic | 40.0% |
| ! | ATAGAAGGAAGCGTACAGTT+AGG | - | chr3.2:71906025-71906044 | None:intergenic | 40.0% |
| ! | TTGTGTTTGATGACCTTGTC+AGG | + | chr3.2:71906292-71906311 | MS.gene77462:CDS | 40.0% |
| !! | GTTTTGAACCTGATCAGCAT+TGG | - | chr3.2:71906210-71906229 | None:intergenic | 40.0% |
| ATACCGCCACATAAACCTTG+TGG | - | chr3.2:71906137-71906156 | None:intergenic | 45.0% | |
| GAAAGTTATAGCAGGACTGC+TGG | - | chr3.2:71906388-71906407 | None:intergenic | 45.0% | |
| TACCTTCTCCAATGCTGATC+AGG | + | chr3.2:71906199-71906218 | MS.gene77462:CDS | 45.0% | |
| TCGGTCTGGAAAGTTATAGC+AGG | - | chr3.2:71906396-71906415 | None:intergenic | 45.0% | |
| TGGAGAAGGTAATGAGACGA+GGG | - | chr3.2:71906190-71906209 | None:intergenic | 45.0% | |
| TTGGAGAAGGTAATGAGACG+AGG | - | chr3.2:71906191-71906210 | None:intergenic | 45.0% | |
| ! | AACCTGATCAGCATTGGAGA+AGG | - | chr3.2:71906204-71906223 | None:intergenic | 45.0% |
| ACTCTTTCCTCGCAGCTTTC+CGG | - | chr3.2:71905888-71905907 | None:intergenic | 50.0% | |
| ATGGCACAGGAACTAACCAC+AGG | - | chr3.2:71906450-71906469 | None:intergenic | 50.0% | |
| CCTCCACAAGGTTTATGTGG+CGG | + | chr3.2:71906131-71906150 | MS.gene77462:CDS | 50.0% | |
| CGCCACATAAACCTTGTGGA+GGG | - | chr3.2:71906133-71906152 | None:intergenic | 50.0% | |
| GACCCTCCACAAGGTTTATG+TGG | + | chr3.2:71906128-71906147 | MS.gene77462:CDS | 50.0% | |
| GTTATAGCAGGACTGCTGGA+AGG | - | chr3.2:71906384-71906403 | None:intergenic | 50.0% | |
| TCAACCCGTCAATGCGTTCA+AGG | + | chr3.2:71906315-71906334 | MS.gene77462:CDS | 50.0% | |
| TTCCTTCACCCAGTCCAGTT+CGG | - | chr3.2:71905949-71905968 | None:intergenic | 50.0% | |
| !! | ATTGACGGGTTGACCTGACA+AGG | - | chr3.2:71906308-71906327 | None:intergenic | 50.0% |
| ATTCGGAACCCGAACTGGAC+TGG | + | chr3.2:71905937-71905956 | MS.gene77462:CDS | 55.0% | |
| CCGCCACATAAACCTTGTGG+AGG | - | chr3.2:71906134-71906153 | None:intergenic | 55.0% | |
| CTCTTTCCTCGCAGCTTTCC+GGG | - | chr3.2:71905887-71905906 | None:intergenic | 55.0% | |
| CTGGGTGAAGGAAGAGGAAG+AGG | + | chr3.2:71905956-71905975 | MS.gene77462:CDS | 55.0% | |
| GAGACGAGGGCGAACATCAT+CGG | - | chr3.2:71906177-71906196 | None:intergenic | 55.0% | |
| GCACAGGAACTAACCACAGG+CGG | - | chr3.2:71906447-71906466 | None:intergenic | 55.0% | |
| TCCTTCACCCAGTCCAGTTC+GGG | - | chr3.2:71905948-71905967 | None:intergenic | 55.0% | |
| TCTTTCCTCGCAGCTTTCCG+GGG | - | chr3.2:71905886-71905905 | None:intergenic | 55.0% | |
| TTAGGCAGGCTTCATGGCAC+AGG | - | chr3.2:71906463-71906482 | None:intergenic | 55.0% | |
| TTCGGAACCCGAACTGGACT+GGG | + | chr3.2:71905938-71905957 | MS.gene77462:CDS | 55.0% | |
| TTCGGATTCGGAACCCGAAC+TGG | + | chr3.2:71905932-71905951 | MS.gene77462:CDS | 55.0% | |
| ! | ACTGGACTGGGTGAAGGAAG+AGG | + | chr3.2:71905950-71905969 | MS.gene77462:CDS | 55.0% |
| !! | TTTGGTGATGCCGTGCAAGC+AGG | - | chr3.2:71905859-71905878 | None:intergenic | 55.0% |
| ACCACAGGCGGTCCATCTGA+AGG | - | chr3.2:71906435-71906454 | None:intergenic | 60.0% | |
| AGGCAGGTATCCGGGGAATC+TGG | - | chr3.2:71905839-71905858 | None:intergenic | 60.0% | |
| CGGATACCTGCCTGCTTGCA+CGG | + | chr3.2:71905846-71905865 | MS.gene77462:CDS | 60.0% | |
| CGGCATCACCAAACCTTCCC+CGG | + | chr3.2:71905866-71905885 | MS.gene77462:CDS | 60.0% | |
| CGTGCAAGCAGGCAGGTATC+CGG | - | chr3.2:71905848-71905867 | None:intergenic | 60.0% | |
| GACGCTATCGACCCTCCACA+AGG | + | chr3.2:71906119-71906138 | MS.gene77462:CDS | 60.0% | |
| GCAGCTTTCCGGGGAAGGTT+TGG | - | chr3.2:71905877-71905896 | None:intergenic | 60.0% | |
| GTGCAAGCAGGCAGGTATCC+GGG | - | chr3.2:71905847-71905866 | None:intergenic | 60.0% | |
| TCCTTCAGATGGACCGCCTG+TGG | + | chr3.2:71906431-71906450 | MS.gene77462:CDS | 60.0% | |
| TGCAAGCAGGCAGGTATCCG+GGG | - | chr3.2:71905846-71905865 | None:intergenic | 60.0% | |
| ! | ACCCGAACTGGACTGGGTGA+AGG | + | chr3.2:71905944-71905963 | MS.gene77462:CDS | 60.0% |
| ACCTTCCCCGGAAAGCTGCG+AGG | + | chr3.2:71905878-71905897 | MS.gene77462:CDS | 65.0% | |
| GTGATGCCGTGCAAGCAGGC+AGG | - | chr3.2:71905855-71905874 | None:intergenic | 65.0% | |
| TCCTCGCAGCTTTCCGGGGA+AGG | - | chr3.2:71905882-71905901 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71905714 | 71906482 | 71905714 | ID=MS.gene77462 |
| chr3.2 | mRNA | 71905714 | 71906482 | 71905714 | ID=MS.gene77462.t1;Parent=MS.gene77462 |
| chr3.2 | exon | 71905714 | 71906028 | 71905714 | ID=MS.gene77462.t1.exon1;Parent=MS.gene77462.t1 |
| chr3.2 | CDS | 71905714 | 71906028 | 71905714 | ID=cds.MS.gene77462.t1;Parent=MS.gene77462.t1 |
| chr3.2 | exon | 71906113 | 71906220 | 71906113 | ID=MS.gene77462.t1.exon2;Parent=MS.gene77462.t1 |
| chr3.2 | CDS | 71906113 | 71906220 | 71906113 | ID=cds.MS.gene77462.t1;Parent=MS.gene77462.t1 |
| chr3.2 | exon | 71906282 | 71906482 | 71906282 | ID=MS.gene77462.t1.exon3;Parent=MS.gene77462.t1 |
| chr3.2 | CDS | 71906282 | 71906482 | 71906282 | ID=cds.MS.gene77462.t1;Parent=MS.gene77462.t1 |
| Gene Sequence |
| Protein sequence |
