AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78529


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78529.t1 MTR_5g078330 75.980 204 4 1 1 159 1 204 2.48e-95 274
MS.gene78529.t1 MTR_3g065570 47.407 135 45 5 18 149 64 175 1.37e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78529.t1 AT1G14900 41.611 149 53 5 17 153 73 199 4.65e-17 75.1

Find 67 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGTCTTGGGAGGTGCATT+AGG 0.195810 5.1:-60624596 None:intergenic
TCAGTCCTTTGAACCTTCTT+TGG 0.198015 5.1:-60624653 None:intergenic
CCTCTCTTTGGTGGTGCATT+TGG 0.255109 5.1:-60624479 None:intergenic
CTTCCACTTGAAGCAGCCTT+AGG 0.283817 5.1:-60624617 None:intergenic
GGTGGAGCCAATGGATCTTT+TGG 0.315588 5.1:-60624521 None:intergenic
GGAGGTGCATTAGGGTCCTT+TGG 0.323687 5.1:-60624587 None:intergenic
GGTCTTCCACGTCCTCTCTT+TGG 0.323730 5.1:-60624491 None:intergenic
CTTAACCAGATGAAGGAAAA+TGG 0.327311 5.1:+60624416 MS.gene78529:intron
TTTGGTGGTGCATTTGGATC+AGG 0.346536 5.1:-60624473 None:intergenic
GGAGGACGACCTCTTGGCCT+TGG 0.354819 5.1:-60624566 None:intergenic
GGGTCTTGGGAGGTGCATTA+GGG 0.356682 5.1:-60624595 None:intergenic
TGAAGCAGCCTTAGGGGTCT+TGG 0.357314 5.1:-60624609 None:intergenic
TTCCACTTGAAGCAGCCTTA+GGG 0.394198 5.1:-60624616 None:intergenic
GACGACCTCTTGGCCTTGGT+GGG 0.404356 5.1:-60624562 None:intergenic
GGTGGAGATGGTGCTGGTGC+TGG 0.421708 5.1:+60624695 MS.gene78529:CDS
CTTCCCCTTCCACTAGTAGT+TGG 0.429832 5.1:-60624776 None:intergenic
GGACGACCTCTTGGCCTTGG+TGG 0.430385 5.1:-60624563 None:intergenic
GAAGCAGCCTTAGGGGTCTT+GGG 0.435245 5.1:-60624608 None:intergenic
TCCTTTGGAGGACGACCTCT+TGG 0.438783 5.1:-60624572 None:intergenic
GACAGAACAGCAGCTGGTGG+TGG 0.460682 5.1:-60624539 None:intergenic
ACTACTAGTGGAAGGGGAAG+GGG 0.460928 5.1:+60624779 MS.gene78529:CDS
GTCATCTGAGGCTTAACCTT+AGG 0.479453 5.1:-60624809 None:intergenic
AACTACTAGTGGAAGGGGAA+GGG 0.479852 5.1:+60624778 MS.gene78529:CDS
TGTGAATTTACCTCAGGGTA+TGG 0.486285 5.1:-60623896 None:intergenic
CTTCCACGTCCTCTCTTTGG+TGG 0.517078 5.1:-60624488 None:intergenic
GCAGCTGGTGGTGGAGCCAA+TGG 0.520984 5.1:-60624530 None:intergenic
GGGAAGGGGTAGGCCTCCTA+AGG 0.528357 5.1:+60624793 MS.gene78529:CDS
AAGGCTGCTTCAAGTGGAAG+AGG 0.531029 5.1:+60624620 MS.gene78529:CDS
ACCCCTAAGGCTGCTTCAAG+TGG 0.532524 5.1:+60624614 MS.gene78529:CDS
ACCAAAGCCAAAAGATCCAT+TGG 0.533769 5.1:+60624514 MS.gene78529:CDS
TCCACTTGAAGCAGCCTTAG+GGG 0.536454 5.1:-60624615 None:intergenic
ATCTGAGGCTTAACCTTAGG+AGG 0.537376 5.1:-60624806 None:intergenic
GCTTCAAGTGGAAGAGGAAG+GGG 0.550156 5.1:+60624626 MS.gene78529:CDS
GGTGGGGACAGAACAGCAGC+TGG 0.550403 5.1:-60624545 None:intergenic
TGCTTCAAGTGGAAGAGGAA+GGG 0.550902 5.1:+60624625 MS.gene78529:CDS
AGGAAGGGGTAGGCCAAAGA+AGG 0.551487 5.1:+60624640 MS.gene78529:CDS
ACAGCAACAGCAGCAACACT+TGG 0.556119 5.1:-60624752 None:intergenic
CAACTACTAGTGGAAGGGGA+AGG 0.556781 5.1:+60624777 MS.gene78529:CDS
GGTGCATTAGGGTCCTTTGG+AGG 0.557332 5.1:-60624584 None:intergenic
CCAGCACCATCTCCACCACT+TGG 0.561242 5.1:-60624689 None:intergenic
GGGTATGGAGGAAGTGAAAG+AGG 0.561822 5.1:-60623881 None:intergenic
CTGCTTCAAGTGGAAGAGGA+AGG 0.565034 5.1:+60624624 MS.gene78529:CDS
AGCTTCAATGGCTACTCAAG+AGG 0.565718 5.1:+60623850 None:intergenic
TGCACCTCCCAAGACCCCTA+AGG 0.569439 5.1:+60624601 MS.gene78529:CDS
CTGTTCCAACTACTAGTGGA+AGG 0.576494 5.1:+60624771 MS.gene78529:CDS
CTGCTGTTCTGTCCCCACCA+AGG 0.579342 5.1:+60624549 MS.gene78529:CDS
TGGTGCTGGTGCTTCTGCTG+AGG 0.584178 5.1:+60624709 MS.gene78529:CDS
GTTCCAACTACTAGTGGAAG+GGG 0.585748 5.1:+60624773 MS.gene78529:CDS
TCAAATCCAAGTGGTGGAGA+TGG 0.596389 5.1:+60624683 MS.gene78529:CDS
GTTGCTGTTCCAACTACTAG+TGG 0.598369 5.1:+60624767 MS.gene78529:CDS
ACGACCTCTTGGCCTTGGTG+GGG 0.598499 5.1:-60624561 None:intergenic
GCCAAGAGGTCGTCCTCCAA+AGG 0.600702 5.1:+60624571 MS.gene78529:CDS
GCACCACCAAAGAGAGGACG+TGG 0.602504 5.1:+60624485 MS.gene78529:CDS
CCAAGTGGTGGAGATGGTGC+TGG 0.603694 5.1:+60624689 MS.gene78529:CDS
AATGTTTCAAATCCAAGTGG+TGG 0.604254 5.1:+60624677 MS.gene78529:CDS
GGGGACAGAACAGCAGCTGG+TGG 0.609403 5.1:-60624542 None:intergenic
TGTTCCAACTACTAGTGGAA+GGG 0.611701 5.1:+60624772 MS.gene78529:CDS
GTAGGCCAAAGAAGGTTCAA+AGG 0.622028 5.1:+60624648 MS.gene78529:CDS
ACACTCACTTCAGTCATCTG+AGG 0.623459 5.1:-60624821 None:intergenic
GCAGCCTTAGGGGTCTTGGG+AGG 0.627198 5.1:-60624605 None:intergenic
GAATTTACCTCAGGGTATGG+AGG 0.638966 5.1:-60623893 None:intergenic
CCAAATGCACCACCAAAGAG+AGG 0.648545 5.1:+60624479 MS.gene78529:CDS
CTAGTGGAAGGGGAAGGGGT+AGG 0.654025 5.1:+60624783 MS.gene78529:CDS
CTGTCCCCACCAAGGCCAAG+AGG 0.676490 5.1:+60624557 MS.gene78529:CDS
TTCACTTCCTCCATACCCTG+AGG 0.676529 5.1:+60623886 MS.gene78529:CDS
GAGAATGTTTCAAATCCAAG+TGG 0.689463 5.1:+60624674 MS.gene78529:CDS
CAAGTGGAAGAGGAAGGGGT+AGG 0.697070 5.1:+60624630 MS.gene78529:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAAGGAATTTTTTTTTA+GGG + chr5.1:60624083-60624102 MS.gene78529:intron 10.0%
!! TTCTTGAAAAAATAAGAAAA+GGG - chr5.1:60623942-60623961 None:intergenic 15.0%
!! TTTCTTGAAAAAATAAGAAA+AGG - chr5.1:60623943-60623962 None:intergenic 15.0%
!!! CAAATAAGGAATTTTTTTTT+AGG + chr5.1:60624082-60624101 MS.gene78529:intron 15.0%
!! AAAATCATTCAACATGTTAA+TGG - chr5.1:60624195-60624214 None:intergenic 20.0%
!! AAATCATTCAACATGTTAAT+GGG - chr5.1:60624194-60624213 None:intergenic 20.0%
!! TCTTGAAAAAATAAGAAAAG+GGG - chr5.1:60623941-60623960 None:intergenic 20.0%
!!! TTAACAATTTTTACTCACTT+GGG + chr5.1:60624012-60624031 MS.gene78529:intron 20.0%
!!! TTTAACAATTTTTACTCACT+TGG + chr5.1:60624011-60624030 MS.gene78529:intron 20.0%
! AACTACATAGAATCAATCTA+TGG + chr5.1:60624353-60624372 MS.gene78529:intron 25.0%
!! GATTTTGTAGATGATTATGA+AGG + chr5.1:60624280-60624299 MS.gene78529:intron 25.0%
!!! GCTGTTTTTGTAATCAAATA+AGG + chr5.1:60624068-60624087 MS.gene78529:intron 25.0%
!!! TACAAAGACTTTTTTCTGTT+TGG - chr5.1:60624125-60624144 None:intergenic 25.0%
AACAGCTCAAGATTACTATT+TGG - chr5.1:60624054-60624073 None:intergenic 30.0%
AGTTGTTCTTTGCAAAAACA+AGG - chr5.1:60624445-60624464 None:intergenic 30.0%
GATTTGCAAGAACAATTGAA+TGG - chr5.1:60624394-60624413 None:intergenic 30.0%
! GTTGAATGATTTTCTCAACA+TGG + chr5.1:60624202-60624221 MS.gene78529:intron 30.0%
! TCAAGATTACTATTTGGTGA+GGG - chr5.1:60624048-60624067 None:intergenic 30.0%
!! GATGATTTGTTTGAACCATT+TGG - chr5.1:60624326-60624345 None:intergenic 30.0%
AAATCACCTTAACCAGATGA+AGG + chr5.1:60624409-60624428 MS.gene78529:intron 35.0%
AATGTTTCAAATCCAAGTGG+TGG + chr5.1:60624677-60624696 MS.gene78529:CDS 35.0%
ATCTATGGTGAATTACCAGA+AGG + chr5.1:60624368-60624387 MS.gene78529:intron 35.0%
CTTAACCAGATGAAGGAAAA+TGG + chr5.1:60624416-60624435 MS.gene78529:intron 35.0%
GAGAATGTTTCAAATCCAAG+TGG + chr5.1:60624674-60624693 MS.gene78529:CDS 35.0%
! AAAGGGGTGTATGTTGAATT+AGG - chr5.1:60623925-60623944 None:intergenic 35.0%
! AAGGGGTGTATGTTGAATTA+GGG - chr5.1:60623924-60623943 None:intergenic 35.0%
! CAAGATTACTATTTGGTGAG+GGG - chr5.1:60624047-60624066 None:intergenic 35.0%
! CATTTTCCTTCATCTGGTTA+AGG - chr5.1:60624418-60624437 None:intergenic 35.0%
! CTCAAGATTACTATTTGGTG+AGG - chr5.1:60624049-60624068 None:intergenic 35.0%
ACCAAAGCCAAAAGATCCAT+TGG + chr5.1:60624514-60624533 MS.gene78529:CDS 40.0%
AGAACAATTGAATGGCCTTC+TGG - chr5.1:60624386-60624405 None:intergenic 40.0%
TCAGTCCTTTGAACCTTCTT+TGG - chr5.1:60624656-60624675 None:intergenic 40.0%
TGTGAATTTACCTCAGGGTA+TGG - chr5.1:60623899-60623918 None:intergenic 40.0%
TGTTCCAACTACTAGTGGAA+GGG + chr5.1:60624772-60624791 MS.gene78529:CDS 40.0%
! AGGGGTGTATGTTGAATTAG+GGG - chr5.1:60623923-60623942 None:intergenic 40.0%
! GTAGATGATTATGAAGGCAC+TGG + chr5.1:60624286-60624305 MS.gene78529:intron 40.0%
!! GAAGCACTGAATGAACCAAA+TGG + chr5.1:60624308-60624327 MS.gene78529:intron 40.0%
!!! AATGGATCTTTTGGCTTTGG+TGG - chr5.1:60624515-60624534 None:intergenic 40.0%
AACTACTAGTGGAAGGGGAA+GGG + chr5.1:60624778-60624797 MS.gene78529:CDS 45.0%
ACACTCACTTCAGTCATCTG+AGG - chr5.1:60624824-60624843 None:intergenic 45.0%
CTGTTCCAACTACTAGTGGA+AGG + chr5.1:60624771-60624790 MS.gene78529:CDS 45.0%
GAATTTACCTCAGGGTATGG+AGG - chr5.1:60623896-60623915 None:intergenic 45.0%
GGGGTGTATGTTGAATTAGG+GGG - chr5.1:60623922-60623941 None:intergenic 45.0%
GTAGGCCAAAGAAGGTTCAA+AGG + chr5.1:60624648-60624667 MS.gene78529:CDS 45.0%
GTTCCAACTACTAGTGGAAG+GGG + chr5.1:60624773-60624792 MS.gene78529:CDS 45.0%
TGCTTCAAGTGGAAGAGGAA+GGG + chr5.1:60624625-60624644 MS.gene78529:CDS 45.0%
TTCCACTTGAAGCAGCCTTA+GGG - chr5.1:60624619-60624638 None:intergenic 45.0%
! ATCTGAGGCTTAACCTTAGG+AGG - chr5.1:60624809-60624828 None:intergenic 45.0%
! GGTCACCATTTTCCTTCATC+TGG - chr5.1:60624424-60624443 None:intergenic 45.0%
! GTCATCTGAGGCTTAACCTT+AGG - chr5.1:60624812-60624831 None:intergenic 45.0%
! GTTGCTGTTCCAACTACTAG+TGG + chr5.1:60624767-60624786 MS.gene78529:CDS 45.0%
! TCAAATCCAAGTGGTGGAGA+TGG + chr5.1:60624683-60624702 MS.gene78529:CDS 45.0%
!! TTTGGTGGTGCATTTGGATC+AGG - chr5.1:60624476-60624495 None:intergenic 45.0%
!!! GCCAATGGATCTTTTGGCTT+TGG - chr5.1:60624518-60624537 None:intergenic 45.0%
AAGGCTGCTTCAAGTGGAAG+AGG + chr5.1:60624620-60624639 MS.gene78529:CDS 50.0%
ACAGCAACAGCAGCAACACT+TGG - chr5.1:60624755-60624774 None:intergenic 50.0%
ACTACTAGTGGAAGGGGAAG+GGG + chr5.1:60624779-60624798 MS.gene78529:CDS 50.0%
CAACTACTAGTGGAAGGGGA+AGG + chr5.1:60624777-60624796 MS.gene78529:CDS 50.0%
CCAAATGCACCACCAAAGAG+AGG + chr5.1:60624479-60624498 MS.gene78529:CDS 50.0%
CTGCTTCAAGTGGAAGAGGA+AGG + chr5.1:60624624-60624643 MS.gene78529:CDS 50.0%
CTTCCACTTGAAGCAGCCTT+AGG - chr5.1:60624620-60624639 None:intergenic 50.0%
CTTCCCCTTCCACTAGTAGT+TGG - chr5.1:60624779-60624798 None:intergenic 50.0%
GCTTCAAGTGGAAGAGGAAG+GGG + chr5.1:60624626-60624645 MS.gene78529:CDS 50.0%
GGGTATGGAGGAAGTGAAAG+AGG - chr5.1:60623884-60623903 None:intergenic 50.0%
TCCACTTGAAGCAGCCTTAG+GGG - chr5.1:60624618-60624637 None:intergenic 50.0%
TTCACTTCCTCCATACCCTG+AGG + chr5.1:60623886-60623905 MS.gene78529:CDS 50.0%
! AGGGGGTGTGAATTTACCTC+AGG - chr5.1:60623905-60623924 None:intergenic 50.0%
! GGGGGTGTGAATTTACCTCA+GGG - chr5.1:60623904-60623923 None:intergenic 50.0%
! GGTGGAGCCAATGGATCTTT+TGG - chr5.1:60624524-60624543 None:intergenic 50.0%
!! CCTCTCTTTGGTGGTGCATT+TGG - chr5.1:60624482-60624501 None:intergenic 50.0%
ACCCCTAAGGCTGCTTCAAG+TGG + chr5.1:60624614-60624633 MS.gene78529:CDS 55.0%
AGGAAGGGGTAGGCCAAAGA+AGG + chr5.1:60624640-60624659 MS.gene78529:CDS 55.0%
CAAGTGGAAGAGGAAGGGGT+AGG + chr5.1:60624630-60624649 MS.gene78529:CDS 55.0%
CTTCCACGTCCTCTCTTTGG+TGG - chr5.1:60624491-60624510 None:intergenic 55.0%
GAAGCAGCCTTAGGGGTCTT+GGG - chr5.1:60624611-60624630 None:intergenic 55.0%
GGTCTTCCACGTCCTCTCTT+TGG - chr5.1:60624494-60624513 None:intergenic 55.0%
GGTGCATTAGGGTCCTTTGG+AGG - chr5.1:60624587-60624606 None:intergenic 55.0%
TGAAGCAGCCTTAGGGGTCT+TGG - chr5.1:60624612-60624631 None:intergenic 55.0%
! GGAGGTGCATTAGGGTCCTT+TGG - chr5.1:60624590-60624609 None:intergenic 55.0%
! GGGTCTTGGGAGGTGCATTA+GGG - chr5.1:60624598-60624617 None:intergenic 55.0%
! TCCTTTGGAGGACGACCTCT+TGG - chr5.1:60624575-60624594 None:intergenic 55.0%
CCAGCACCATCTCCACCACT+TGG - chr5.1:60624692-60624711 None:intergenic 60.0%
CTAGTGGAAGGGGAAGGGGT+AGG + chr5.1:60624783-60624802 MS.gene78529:CDS 60.0%
CTGCTGTTCTGTCCCCACCA+AGG + chr5.1:60624549-60624568 MS.gene78529:CDS 60.0%
GACAGAACAGCAGCTGGTGG+TGG - chr5.1:60624542-60624561 None:intergenic 60.0%
GCACCACCAAAGAGAGGACG+TGG + chr5.1:60624485-60624504 MS.gene78529:CDS 60.0%
GCCAAGAGGTCGTCCTCCAA+AGG + chr5.1:60624571-60624590 MS.gene78529:CDS 60.0%
TGCACCTCCCAAGACCCCTA+AGG + chr5.1:60624601-60624620 MS.gene78529:CDS 60.0%
! CCAAGTGGTGGAGATGGTGC+TGG + chr5.1:60624689-60624708 MS.gene78529:CDS 60.0%
! GGGGTCTTGGGAGGTGCATT+AGG - chr5.1:60624599-60624618 None:intergenic 60.0%
!! ACGACCTCTTGGCCTTGGTG+GGG - chr5.1:60624564-60624583 None:intergenic 60.0%
!! GACGACCTCTTGGCCTTGGT+GGG - chr5.1:60624565-60624584 None:intergenic 60.0%
!! TGGTGCTGGTGCTTCTGCTG+AGG + chr5.1:60624709-60624728 MS.gene78529:CDS 60.0%
CTGTCCCCACCAAGGCCAAG+AGG + chr5.1:60624557-60624576 MS.gene78529:CDS 65.0%
GCAGCCTTAGGGGTCTTGGG+AGG - chr5.1:60624608-60624627 None:intergenic 65.0%
GGAGGACGACCTCTTGGCCT+TGG - chr5.1:60624569-60624588 None:intergenic 65.0%
GGGAAGGGGTAGGCCTCCTA+AGG + chr5.1:60624793-60624812 MS.gene78529:CDS 65.0%
GGGGACAGAACAGCAGCTGG+TGG - chr5.1:60624545-60624564 None:intergenic 65.0%
GGTGGGGACAGAACAGCAGC+TGG - chr5.1:60624548-60624567 None:intergenic 65.0%
!! GCAGCTGGTGGTGGAGCCAA+TGG - chr5.1:60624533-60624552 None:intergenic 65.0%
!! GGACGACCTCTTGGCCTTGG+TGG - chr5.1:60624566-60624585 None:intergenic 65.0%
!! GGTGGAGATGGTGCTGGTGC+TGG + chr5.1:60624695-60624714 MS.gene78529:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 60623857 60624853 60623857 ID=MS.gene78529
chr5.1 mRNA 60623857 60624853 60623857 ID=MS.gene78529.t1;Parent=MS.gene78529
chr5.1 exon 60623857 60623907 60623857 ID=MS.gene78529.t1.exon1;Parent=MS.gene78529.t1
chr5.1 CDS 60623857 60623907 60623857 ID=cds.MS.gene78529.t1;Parent=MS.gene78529.t1
chr5.1 exon 60624425 60624853 60624425 ID=MS.gene78529.t1.exon2;Parent=MS.gene78529.t1
chr5.1 CDS 60624425 60624853 60624425 ID=cds.MS.gene78529.t1;Parent=MS.gene78529.t1
Gene Sequence

>MS.gene78529

ATGGCTACTCAAGAGGTTAATAAGCCTCTTTCACTTCCTCCATACCCTGAGGTAAATTCACACCCCCTAATTCAACATACACCCCTTTTCTTATTTTTTCAAGAAAATTAGTCTTTCATAGTAAAAGTAGCAATTTTTTCACTGATCTGTTTGATTTAACAATTTTTACTCACTTGGGTACTTTTTTCCCCTCACCAAATAGTAATCTTGAGCTGTTTTTGTAATCAAATAAGGAATTTTTTTTTAGGGTTTATGAAGAATTTAACCAAACAGAAAAAAGTCTTTGTATTAATTTCAGCTCAATTAATCTTGAAATTCTTTGTTTTTTCACTAACCCATTAACATGTTGAATGATTTTCTCAACATGGTATCTTCATTTCTGAAAAAAAATTTGATTTTTATTTTTATTTTAACTCTGTTGTTGATTTTGTAGATGATTATGAAGGCACTGGAAGCACTGAATGAACCAAATGGTTCAAACAAATCATCAATTTCAAACTACATAGAATCAATCTATGGTGAATTACCAGAAGGCCATTCAATTGTTCTTGCAAATCACCTTAACCAGATGAAGGAAAATGGTGACCTTGTTTTTGCAAAGAACAACTACATGAAGCCTGATCCAAATGCACCACCAAAGAGAGGACGTGGAAGACCACCAAAGCCAAAAGATCCATTGGCTCCACCACCAGCTGCTGTTCTGTCCCCACCAAGGCCAAGAGGTCGTCCTCCAAAGGACCCTAATGCACCTCCCAAGACCCCTAAGGCTGCTTCAAGTGGAAGAGGAAGGGGTAGGCCAAAGAAGGTTCAAAGGACTGAGAATGTTTCAAATCCAAGTGGTGGAGATGGTGCTGGTGCTGGTGCTTCTGCTGAGGTTGATGATGATGATGCTGCTCCAAGTGTTGCTGCTGTTGCTGTTCCAACTACTAGTGGAAGGGGAAGGGGTAGGCCTCCTAAGGTTAAGCCTCAGATGACTGAAGTGAGTGTTGAATCATAG

Protein sequence

>MS.gene78529.t1

MATQEVNKPLSLPPYPEMKENGDLVFAKNNYMKPDPNAPPKRGRGRPPKPKDPLAPPPAAVLSPPRPRGRPPKDPNAPPKTPKAASSGRGRGRPKKVQRTENVSNPSGGDGAGAGASAEVDDDDAAPSVAAVAVPTTSGRGRGRPPKVKPQMTEVSVES