AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78880.t1 MTR_8g041770 89.904 208 21 0 1 208 234 441 4.30e-136 389
MS.gene78880.t1 MTR_0190s0020 75.000 208 52 0 1 208 235 442 3.46e-111 325
MS.gene78880.t1 MTR_4g022825 62.500 208 75 2 1 207 234 439 2.06e-90 272
MS.gene78880.t1 MTR_3g088420 61.722 209 77 2 1 208 227 433 4.39e-89 268
MS.gene78880.t1 MTR_4g022720 62.201 209 76 2 1 208 229 435 9.10e-89 268
MS.gene78880.t1 MTR_4g022800 62.381 210 75 2 1 208 235 442 1.85e-88 267
MS.gene78880.t1 MTR_8g040780 60.680 206 78 2 1 203 22 227 2.04e-87 258
MS.gene78880.t1 MTR_4g022670 60.766 209 79 2 1 208 235 441 2.17e-87 265
MS.gene78880.t1 MTR_3g026310 58.294 211 83 2 1 208 240 448 1.39e-85 260
MS.gene78880.t1 MTR_4g094835 60.287 209 77 3 1 204 235 442 2.16e-85 259
MS.gene78880.t1 MTR_4g094832 60.287 209 77 3 1 204 219 426 1.80e-84 256
MS.gene78880.t1 MTR_7g026860 60.096 208 79 2 1 208 235 438 4.87e-83 253
MS.gene78880.t1 MTR_4g094842 56.311 206 90 0 1 206 241 446 1.85e-81 249
MS.gene78880.t1 MTR_4g094838 58.373 209 81 3 1 204 221 428 8.65e-81 247
MS.gene78880.t1 MTR_4g094828 49.309 217 100 5 1 208 235 450 7.48e-63 201
MS.gene78880.t1 MTR_4g022760 49.038 208 61 1 1 208 235 397 6.03e-62 198
MS.gene78880.t1 MTR_8g079710 49.282 209 96 5 1 199 228 436 4.42e-59 192
MS.gene78880.t1 MTR_4g022290 46.789 218 103 5 1 206 227 443 7.02e-54 178
MS.gene78880.t1 MTR_4g094825 52.980 151 59 2 55 204 248 387 8.91e-48 161
MS.gene78880.t1 MTR_3g101900 40.191 209 122 3 2 208 241 448 2.85e-46 158
MS.gene78880.t1 MTR_6g016815 43.750 208 55 4 1 208 236 381 2.58e-45 154
MS.gene78880.t1 MTR_3g102020 40.097 207 121 3 1 205 243 448 5.93e-45 155
MS.gene78880.t1 MTR_3g101930 38.942 208 125 2 1 207 248 454 2.82e-44 153
MS.gene78880.t1 MTR_3g101970 38.278 209 126 3 1 207 229 436 4.68e-42 147
MS.gene78880.t1 MTR_3g101960 39.024 205 122 3 1 203 242 445 1.17e-41 146
MS.gene78880.t1 MTR_3g102010 39.048 210 125 3 1 208 243 451 2.20e-41 145
MS.gene78880.t1 MTR_6g034485 69.767 86 26 0 1 86 40 125 1.78e-38 129
MS.gene78880.t1 MTR_7g026770 56.731 104 45 0 1 104 198 301 1.44e-33 122
MS.gene78880.t1 MTR_5g081960 34.536 194 125 2 16 208 294 486 3.42e-33 124
MS.gene78880.t1 MTR_4g015180 36.216 185 116 2 25 208 304 487 7.24e-32 120
MS.gene78880.t1 MTR_3g070170 34.783 184 118 2 22 204 284 466 5.11e-30 115
MS.gene78880.t1 MTR_6g045553 31.731 208 137 3 1 204 265 471 1.09e-27 108
MS.gene78880.t1 MTR_7g026780 46.847 111 34 2 98 208 26 111 1.21e-24 93.6
MS.gene78880.t1 MTR_3g102030 33.129 163 90 3 1 162 176 320 2.02e-21 90.1
MS.gene78880.t1 MTR_7g026870 54.286 70 27 1 53 117 82 151 6.55e-18 77.4
MS.gene78880.t1 MTR_4g007540 25.126 199 144 3 1 195 227 424 2.38e-17 79.7
MS.gene78880.t1 MTR_8g075610 24.631 203 138 3 1 195 216 411 7.58e-16 75.5
MS.gene78880.t1 MTR_2g064495 24.242 198 147 2 1 195 226 423 2.99e-15 73.9
MS.gene78880.t1 MTR_2g105330 24.242 198 147 2 1 195 226 423 6.63e-15 72.8
MS.gene78880.t1 MTR_4g127650 23.364 214 146 3 1 197 236 448 2.53e-14 71.2
MS.gene78880.t1 MTR_3g026950 26.941 219 122 7 1 192 229 436 5.70e-14 70.1
MS.gene78880.t1 MTR_3g049150 25.000 212 125 6 1 189 229 429 1.59e-13 68.9
MS.gene78880.t1 MTR_3g098980 25.532 188 133 3 2 189 238 418 1.87e-13 68.6
MS.gene78880.t1 MTR_3g049140 24.519 208 136 7 1 192 27 229 2.63e-13 67.0
MS.gene78880.t1 MTR_7g026880 55.556 54 22 1 155 208 2 53 6.42e-13 61.6
MS.gene78880.t1 MTR_4g097090 25.728 206 135 6 1 189 236 440 9.15e-12 63.5
MS.gene78880.t1 MTR_6g015320 25.500 200 138 5 1 195 251 444 1.29e-11 63.2
MS.gene78880.t1 MTR_8g096530 27.848 158 105 3 2 157 232 382 2.08e-11 62.8
MS.gene78880.t1 MTR_6g015315 24.883 213 130 8 1 198 248 445 3.04e-11 62.0
MS.gene78880.t1 MTR_6g015850 25.871 201 140 5 1 198 256 450 5.04e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78880.t1 AT5G67150 41.429 210 119 3 1 208 239 446 6.03e-53 176
MS.gene78880.t1 AT3G50270 39.906 213 122 3 1 208 237 448 5.24e-48 163
MS.gene78880.t1 AT5G67160 43.062 209 112 5 1 208 230 432 1.25e-47 162
MS.gene78880.t1 AT3G50280 40.000 210 120 3 1 205 102 310 8.25e-47 156
MS.gene78880.t1 AT3G50300 43.590 195 104 3 1 190 232 425 4.28e-46 158
MS.gene78880.t1 AT3G50280 40.000 210 120 3 1 205 232 440 1.23e-45 157
MS.gene78880.t1 AT5G07860 40.670 209 119 4 1 207 243 448 3.08e-43 150
MS.gene78880.t1 AT5G42830 38.571 210 126 3 1 208 241 449 4.71e-41 145
MS.gene78880.t1 AT5G01210 33.469 245 125 4 1 208 226 469 7.14e-39 139
MS.gene78880.t1 AT5G07870 39.151 212 122 4 1 208 253 461 3.24e-38 137
MS.gene78880.t1 AT5G38130 40.206 194 110 3 1 189 250 442 1.57e-37 135
MS.gene78880.t1 AT3G50290 46.875 160 79 3 1 155 111 269 9.52e-37 129
MS.gene78880.t1 AT5G07850 38.532 218 116 7 1 208 244 453 1.51e-35 130
MS.gene78880.t1 AT2G39980 37.838 185 113 2 25 208 297 480 1.84e-34 127
MS.gene78880.t1 AT5G23940 31.250 208 138 3 1 204 242 448 6.06e-29 112
MS.gene78880.t1 AT5G48930 25.628 199 143 3 1 195 227 424 2.81e-16 76.6
MS.gene78880.t1 AT2G19070 28.302 212 135 5 1 197 234 443 8.73e-16 75.5
MS.gene78880.t1 AT3G29590 23.383 201 151 2 2 199 242 442 1.63e-12 65.9
MS.gene78880.t1 AT5G39090 29.208 202 116 9 2 190 243 430 3.75e-12 64.7
MS.gene78880.t1 AT5G61160 26.633 199 128 6 5 195 250 438 3.88e-12 64.7
MS.gene78880.t1 AT1G28680 27.950 161 108 4 2 160 235 389 6.50e-11 61.2

Find 43 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCCTGGTTTAATGTTTA+TGG 0.116946 8.3:-56350293 MS.gene78880:CDS
GGAAATATTTGTGTGTTTAA+AGG 0.251922 8.3:-56350209 MS.gene78880:CDS
TGGTAATGATCCTGAGTTTA+TGG 0.261735 8.3:-56350126 MS.gene78880:CDS
GGGAGTGAAAATAATGAAAA+TGG 0.264658 8.3:-56350230 MS.gene78880:CDS
CCATTGAAAGAGGATTATTT+TGG 0.285934 8.3:-56350521 MS.gene78880:CDS
AATGTTTATGGTAATGATTT+TGG 0.340175 8.3:-56350281 MS.gene78880:CDS
GTGCATTATGTGGTGGTTAT+AGG 0.349094 8.3:-56350566 MS.gene78880:CDS
AAAATTCAAAGCCAACTTAG+AGG 0.353981 8.3:-56350681 MS.gene78880:CDS
CCAAAATAATCCTCTTTCAA+TGG 0.374389 8.3:+56350521 None:intergenic
CAATTGCCTCCAAAGTTTCA+TGG 0.386784 8.3:+56350147 None:intergenic
TGGACTTGGTAAAGGTGCTT+TGG 0.409489 8.3:-56350441 MS.gene78880:CDS
CATGAAACTTTGGAGGCAAT+TGG 0.418429 8.3:-56350146 MS.gene78880:CDS
AGGTGTAGAAGATGGTAGTA+TGG 0.424099 8.3:-56350189 MS.gene78880:CDS
TTTGGTTGGGGAAAACCTGT+GGG 0.472290 8.3:-56350263 MS.gene78880:CDS
GTTGATTCCTCCATTGAAAG+AGG 0.486725 8.3:-56350531 MS.gene78880:CDS
CTTTGTACCAGCCTCTAAGT+TGG 0.486862 8.3:+56350670 None:intergenic
CATAATGCACTTCTTCTTGA+GGG 0.502724 8.3:+56350579 None:intergenic
CAAGACAATGGACTTGGTAA+AGG 0.507284 8.3:-56350449 MS.gene78880:CDS
TTCAAAGCCAACTTAGAGGC+TGG 0.516313 8.3:-56350677 MS.gene78880:CDS
GCACTTCTTCTTGAGGGTCA+AGG 0.528530 8.3:+56350585 None:intergenic
TTCAATGGAGGAATCAACCT+TGG 0.531124 8.3:+56350536 None:intergenic
AATTGCCTCCAAAGTTTCAT+GGG 0.532415 8.3:+56350148 None:intergenic
GTGTTTGTCCCATGAAACTT+TGG 0.534872 8.3:-56350156 MS.gene78880:CDS
GTACAATAGTAAAACATTAG+TGG 0.537819 8.3:-56350324 MS.gene78880:CDS
TACAATAGTAAAACATTAGT+GGG 0.542055 8.3:-56350323 MS.gene78880:CDS
TCCGTTGTTAGCATGAAAGT+TGG 0.545219 8.3:-56350482 MS.gene78880:CDS
ACATAATGCACTTCTTCTTG+AGG 0.564314 8.3:+56350578 None:intergenic
TTGGTTGGGGAAAACCTGTG+GGG 0.567130 8.3:-56350262 MS.gene78880:CDS
TGGTTGGGGAAAACCTGTGG+GGG 0.578502 8.3:-56350261 MS.gene78880:CDS
TTTGTCCCATGAAACTTTGG+AGG 0.591981 8.3:-56350153 MS.gene78880:CDS
AAATAATCCTCTTTCAATGG+AGG 0.592205 8.3:+56350524 None:intergenic
GTGTTTAAAGGTGTAGAAGA+TGG 0.594759 8.3:-56350197 MS.gene78880:CDS
TTGTTGCAAGACAATGGACT+TGG 0.601437 8.3:-56350455 MS.gene78880:CDS
ATACCAAGAGTAATAAAACT+AGG 0.607573 8.3:+56350347 None:intergenic
GGTGATTTGTTGCAAGACAA+TGG 0.609643 8.3:-56350461 MS.gene78880:CDS
TGGTTATAGGCGTTAGACCA+AGG 0.625694 8.3:-56350553 MS.gene78880:CDS
TCAAGAAGAAGTGCATTATG+TGG 0.629418 8.3:-56350576 MS.gene78880:CDS
GTCACAACATCCATAAACTC+AGG 0.634083 8.3:+56350116 None:intergenic
AGAAGAAGTGCATTATGTGG+TGG 0.641210 8.3:-56350573 MS.gene78880:CDS
TAGTGGGTAGTAGTTCACCC+TGG 0.662355 8.3:-56350307 MS.gene78880:CDS
ATTACCATAAACATTAAACC+AGG 0.672703 8.3:+56350289 None:intergenic
ACCAACTTTCATGCTAACAA+CGG 0.674514 8.3:+56350481 None:intergenic
TTACCATAAACATTAAACCA+GGG 0.701842 8.3:+56350290 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAATAGTAAAACATTAGT+GGG - chr8.3:56350506-56350525 MS.gene78880:CDS 20.0%
!!! AATGTTTATGGTAATGATTT+TGG - chr8.3:56350548-56350567 MS.gene78880:CDS 20.0%
! ATACCAAGAGTAATAAAACT+AGG + chr8.3:56350485-56350504 None:intergenic 25.0%
! ATTACCATAAACATTAAACC+AGG + chr8.3:56350543-56350562 None:intergenic 25.0%
! GGAAATATTTGTGTGTTTAA+AGG - chr8.3:56350620-56350639 MS.gene78880:CDS 25.0%
! GTACAATAGTAAAACATTAG+TGG - chr8.3:56350505-56350524 MS.gene78880:CDS 25.0%
! TTACCATAAACATTAAACCA+GGG + chr8.3:56350542-56350561 None:intergenic 25.0%
!!! TTATGGTAATGATTTTGGTT+GGG - chr8.3:56350553-56350572 MS.gene78880:CDS 25.0%
!!! TTTATGGTAATGATTTTGGT+TGG - chr8.3:56350552-56350571 MS.gene78880:CDS 25.0%
AAAATTCAAAGCCAACTTAG+AGG - chr8.3:56350148-56350167 MS.gene78880:CDS 30.0%
AAATAATCCTCTTTCAATGG+AGG + chr8.3:56350308-56350327 None:intergenic 30.0%
CCAAAATAATCCTCTTTCAA+TGG + chr8.3:56350311-56350330 None:intergenic 30.0%
GGGAGTGAAAATAATGAAAA+TGG - chr8.3:56350599-56350618 MS.gene78880:CDS 30.0%
TGAAAAATGACTATGAGAGT+TGG - chr8.3:56350450-56350469 MS.gene78880:CDS 30.0%
! AAGCACTTTTCACACATATT+TGG - chr8.3:56350198-56350217 MS.gene78880:CDS 30.0%
! CCATTGAAAGAGGATTATTT+TGG - chr8.3:56350308-56350327 MS.gene78880:CDS 30.0%
!!! ACACCTAGTTTTATTACTCT+TGG - chr8.3:56350479-56350498 MS.gene78880:CDS 30.0%
!!! TATGGTAATGATTTTGGTTG+GGG - chr8.3:56350554-56350573 MS.gene78880:CDS 30.0%
AATTGCCTCCAAAGTTTCAT+GGG + chr8.3:56350684-56350703 None:intergenic 35.0%
ACATAATGCACTTCTTCTTG+AGG + chr8.3:56350254-56350273 None:intergenic 35.0%
ACCAACTTTCATGCTAACAA+CGG + chr8.3:56350351-56350370 None:intergenic 35.0%
CATAATGCACTTCTTCTTGA+GGG + chr8.3:56350253-56350272 None:intergenic 35.0%
TCACCCTGGTTTAATGTTTA+TGG - chr8.3:56350536-56350555 MS.gene78880:CDS 35.0%
! GTGTTTAAAGGTGTAGAAGA+TGG - chr8.3:56350632-56350651 MS.gene78880:CDS 35.0%
! TCAAGAAGAAGTGCATTATG+TGG - chr8.3:56350253-56350272 MS.gene78880:CDS 35.0%
! TGGTAATGATCCTGAGTTTA+TGG - chr8.3:56350703-56350722 MS.gene78880:CDS 35.0%
AGGTGTAGAAGATGGTAGTA+TGG - chr8.3:56350640-56350659 MS.gene78880:CDS 40.0%
CAATTGCCTCCAAAGTTTCA+TGG + chr8.3:56350685-56350704 None:intergenic 40.0%
GTCACAACATCCATAAACTC+AGG + chr8.3:56350716-56350735 None:intergenic 40.0%
GTGCATTATGTGGTGGTTAT+AGG - chr8.3:56350263-56350282 MS.gene78880:CDS 40.0%
GTGTTTGTCCCATGAAACTT+TGG - chr8.3:56350673-56350692 MS.gene78880:CDS 40.0%
TCCGTTGTTAGCATGAAAGT+TGG - chr8.3:56350347-56350366 MS.gene78880:CDS 40.0%
TTCAATGGAGGAATCAACCT+TGG + chr8.3:56350296-56350315 None:intergenic 40.0%
TTTGTCCCATGAAACTTTGG+AGG - chr8.3:56350676-56350695 MS.gene78880:CDS 40.0%
! AGAAGAAGTGCATTATGTGG+TGG - chr8.3:56350256-56350275 MS.gene78880:CDS 40.0%
! CAAGACAATGGACTTGGTAA+AGG - chr8.3:56350380-56350399 MS.gene78880:CDS 40.0%
! CATGAAACTTTGGAGGCAAT+TGG - chr8.3:56350683-56350702 MS.gene78880:CDS 40.0%
! GTTGATTCCTCCATTGAAAG+AGG - chr8.3:56350298-56350317 MS.gene78880:CDS 40.0%
! TTCTTGAGGGTCAAGGTTTT+TGG + chr8.3:56350240-56350259 None:intergenic 40.0%
! TTGTTGCAAGACAATGGACT+TGG - chr8.3:56350374-56350393 MS.gene78880:CDS 40.0%
!! GGTGATTTGTTGCAAGACAA+TGG - chr8.3:56350368-56350387 MS.gene78880:CDS 40.0%
CTTTGTACCAGCCTCTAAGT+TGG + chr8.3:56350162-56350181 None:intergenic 45.0%
TGGTTATAGGCGTTAGACCA+AGG - chr8.3:56350276-56350295 MS.gene78880:CDS 45.0%
TTCAAAGCCAACTTAGAGGC+TGG - chr8.3:56350152-56350171 MS.gene78880:CDS 45.0%
TTTGGTTGGGGAAAACCTGT+GGG - chr8.3:56350566-56350585 MS.gene78880:CDS 45.0%
!! TGGACTTGGTAAAGGTGCTT+TGG - chr8.3:56350388-56350407 MS.gene78880:CDS 45.0%
!! TTTTGGTTGGGGAAAACCTG+TGG - chr8.3:56350565-56350584 MS.gene78880:CDS 45.0%
GCACTTCTTCTTGAGGGTCA+AGG + chr8.3:56350247-56350266 None:intergenic 50.0%
TTGGTTGGGGAAAACCTGTG+GGG - chr8.3:56350567-56350586 MS.gene78880:CDS 50.0%
! TAGTGGGTAGTAGTTCACCC+TGG - chr8.3:56350522-56350541 MS.gene78880:CDS 50.0%
AAACCTGTGGGGGTTCGAAC+TGG - chr8.3:56350578-56350597 MS.gene78880:CDS 55.0%
AACCTGTGGGGGTTCGAACT+GGG - chr8.3:56350579-56350598 MS.gene78880:CDS 55.0%
TGGTTGGGGAAAACCTGTGG+GGG - chr8.3:56350568-56350587 MS.gene78880:CDS 55.0%
CTCCCAGTTCGAACCCCCAC+AGG + chr8.3:56350584-56350603 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 56350113 56350738 56350113 ID=MS.gene78880
chr8.3 mRNA 56350113 56350738 56350113 ID=MS.gene78880.t1;Parent=MS.gene78880
chr8.3 exon 56350113 56350738 56350113 ID=MS.gene78880.t1.exon1;Parent=MS.gene78880.t1
chr8.3 CDS 56350113 56350738 56350113 ID=cds.MS.gene78880.t1;Parent=MS.gene78880.t1
Gene Sequence

>MS.gene78880

ATGTTTCATTTCACAAAAGAGAATATTGCAAAACTAAAATTCAAAGCCAACTTAGAGGCTGGTACAAAGAACATATCTTCTTTGCAAGCACTTTTCACACATATTTGGCGATCTATTATACGTTCCAAAAACCTTGACCCTCAAGAAGAAGTGCATTATGTGGTGGTTATAGGCGTTAGACCAAGGTTGATTCCTCCATTGAAAGAGGATTATTTTGGTAATGCTATGATAGACTCCGTTGTTAGCATGAAAGTTGGTGATTTGTTGCAAGACAATGGACTTGGTAAAGGTGCTTTGGAGATGAACAAGATGATTGCTTTGCAATCTGATGAGATGTTGAAAAATGACTATGAGAGTTGGTTGAAAACACCTAGTTTTATTACTCTTGGTATGTACAATAGTAAAACATTAGTGGGTAGTAGTTCACCCTGGTTTAATGTTTATGGTAATGATTTTGGTTGGGGAAAACCTGTGGGGGTTCGAACTGGGAGTGAAAATAATGAAAATGGAAATATTTGTGTGTTTAAAGGTGTAGAAGATGGTAGTATGGATCTTGAAGTGTGTTTGTCCCATGAAACTTTGGAGGCAATTGGTAATGATCCTGAGTTTATGGATGTTGTGACCAA

Protein sequence

>MS.gene78880.t1

MFHFTKENIAKLKFKANLEAGTKNISSLQALFTHIWRSIIRSKNLDPQEEVHYVVVIGVRPRLIPPLKEDYFGNAMIDSVVSMKVGDLLQDNGLGKGALEMNKMIALQSDEMLKNDYESWLKTPSFITLGMYNSKTLVGSSSPWFNVYGNDFGWGKPVGVRTGSENNENGNICVFKGVEDGSMDLEVCLSHETLEAIGNDPEFMDVVT