Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060314.t1 | KEH44081.1 | 95.2 | 83 | 4 | 0 | 1 | 83 | 220 | 302 | 1.70E-38 | 168.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060314.t1 | Q71DJ5 | 57.9 | 76 | 32 | 0 | 4 | 79 | 211 | 286 | 1.1e-22 | 107.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060314.t1 | A0A072VQX1 | 95.2 | 83 | 4 | 0 | 1 | 83 | 220 | 302 | 1.2e-38 | 168.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050558 | MS.gene060314 | 0.801389 | 9.22E-49 | -1.69E-46 |
| MS.gene050560 | MS.gene060314 | 0.807745 | 4.35E-50 | -1.69E-46 |
| MS.gene050787 | MS.gene060314 | 0.801874 | 7.33E-49 | -1.69E-46 |
| MS.gene052207 | MS.gene060314 | 0.80592 | 1.06E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene060314 | 0.81003 | 1.41E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene060314 | 0.83944 | 1.58E-57 | -1.69E-46 |
| MS.gene060314 | MS.gene063865 | 0.808758 | 2.65E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene065926 | 0.800275 | 1.56E-48 | -1.69E-46 |
| MS.gene060314 | MS.gene068950 | 0.806957 | 6.39E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene069680 | 0.802531 | 5.37E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene07115 | 0.808309 | 3.30E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene26073 | 0.81345 | 2.54E-51 | -1.69E-46 |
| MS.gene060314 | MS.gene31609 | 0.804733 | 1.87E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene34352 | 0.803031 | 4.23E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene44649 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene48577 | 0.818064 | 2.38E-52 | -1.69E-46 |
| MS.gene060314 | MS.gene49304 | 0.801513 | 8.69E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene50621 | 0.815237 | 1.02E-51 | -1.69E-46 |
| MS.gene060314 | MS.gene51570 | 0.804613 | 1.99E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene56053 | 0.800031 | 1.74E-48 | -1.69E-46 |
| MS.gene060314 | MS.gene59149 | 0.81129 | 7.54E-51 | -1.69E-46 |
| MS.gene060314 | MS.gene63507 | 0.825323 | 4.99E-54 | -1.69E-46 |
| MS.gene060314 | MS.gene71456 | 0.801134 | 1.04E-48 | -1.69E-46 |
| MS.gene060314 | MS.gene75304 | 0.842038 | 3.30E-58 | -1.69E-46 |
| MS.gene060314 | MS.gene76554 | 0.827813 | 1.27E-54 | -1.69E-46 |
| MS.gene060314 | MS.gene78880 | 0.815426 | 9.30E-52 | -1.69E-46 |
| MS.gene060314 | MS.gene79217 | 0.802891 | 4.52E-49 | -1.69E-46 |
| MS.gene060314 | MS.gene86055 | 0.809779 | 1.60E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene91645 | 0.852462 | 4.54E-61 | -1.69E-46 |
| MS.gene060314 | MS.gene93892 | 0.806144 | 9.49E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene97224 | 0.80824 | 3.41E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene99735 | 0.80011 | 1.68E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060314.t1 | MTR_1g107330 | 95.181 | 83 | 4 | 0 | 1 | 83 | 220 | 302 | 3.00e-53 | 169 |
| MS.gene060314.t1 | MTR_1g107330 | 94.048 | 84 | 5 | 0 | 1 | 84 | 220 | 303 | 1.48e-52 | 169 |
| MS.gene060314.t1 | MTR_5g049630 | 90.625 | 32 | 3 | 0 | 48 | 79 | 34 | 65 | 3.87e-16 | 67.8 |
| MS.gene060314.t1 | MTR_5g049820 | 90.625 | 32 | 3 | 0 | 48 | 79 | 903 | 934 | 3.19e-15 | 69.3 |
| MS.gene060314.t1 | MTR_7g451290 | 85.714 | 35 | 2 | 1 | 49 | 80 | 65 | 99 | 3.31e-14 | 63.2 |
| MS.gene060314.t1 | MTR_7g029365 | 40.476 | 84 | 47 | 2 | 3 | 83 | 229 | 312 | 5.41e-13 | 63.2 |
| MS.gene060314.t1 | MTR_7g029365 | 39.744 | 78 | 46 | 1 | 3 | 79 | 229 | 306 | 1.43e-12 | 61.6 |
| MS.gene060314.t1 | MTR_7g081050 | 41.096 | 73 | 40 | 2 | 13 | 82 | 232 | 304 | 5.09e-12 | 60.5 |
| MS.gene060314.t1 | MTR_7g081060 | 42.647 | 68 | 38 | 1 | 13 | 79 | 232 | 299 | 6.44e-12 | 60.1 |
| MS.gene060314.t1 | MTR_6g033965 | 43.421 | 76 | 42 | 1 | 10 | 84 | 229 | 304 | 8.22e-12 | 59.7 |
| MS.gene060314.t1 | MTR_3g081040 | 35.955 | 89 | 46 | 4 | 13 | 90 | 232 | 320 | 2.53e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060314.t1 | AT2G15230 | 54.762 | 84 | 38 | 0 | 1 | 84 | 208 | 291 | 1.10e-29 | 108 |
Find 0 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTGATGTAAAAAATAT+AGG | + | 8684:3133-3152 | MS.gene060314:intron | 10.0% |
| !!! | AGAAAAAAACAATTTTATTT+AGG | + | 8684:3520-3539 | MS.gene060314:intron | 10.0% |
| !!! | ATATTTTTTACATCAATAAT+GGG | - | 8684:3133-3152 | MS.gene060314:intergenic | 10.0% |
| !!! | TATATTTTTTACATCAATAA+TGG | - | 8684:3134-3153 | MS.gene060314:intergenic | 10.0% |
| !! | ATAAATTAATATATGTGTAG+CGG | - | 8684:3168-3187 | MS.gene060314:intergenic | 15.0% |
| !! | ATGACATTAAATTAAATTAC+AGG | + | 8684:3777-3796 | MS.gene060314:intron | 15.0% |
| !! | GATAGTAGAAGAATAAATAA+AGG | - | 8684:3295-3314 | MS.gene060314:intergenic | 20.0% |
| !!! | ATTATGAGAAGAGTATTATT+TGG | - | 8684:2963-2982 | MS.gene060314:intergenic | 20.0% |
| !!! | CTCATTTTAATCAATAACTT+AGG | + | 8684:3671-3690 | MS.gene060314:intron | 20.0% |
| !!! | TGAGAAATTTTGATCAATTA+GGG | - | 8684:3361-3380 | MS.gene060314:intergenic | 20.0% |
| !!! | TTGAGAAATTTTGATCAATT+AGG | - | 8684:3362-3381 | MS.gene060314:intergenic | 20.0% |
| ! | AAGAAACCAATTAAAGATGA+TGG | - | 8684:3912-3931 | MS.gene060314:intergenic | 25.0% |
| ! | ACATTATCAAACATGATCAA+AGG | - | 8684:4075-4094 | MS.gene060314:intergenic | 25.0% |
| ! | ATATGTAACTCTTGTATCTA+CGG | + | 8684:3385-3404 | MS.gene060314:intron | 25.0% |
| ! | ATCAACATATGCAAAAGAAA+AGG | - | 8684:2683-2702 | MS.gene060314:intergenic | 25.0% |
| ! | TAAATAAAGGTCAGATACTA+TGG | - | 8684:3282-3301 | MS.gene060314:intergenic | 25.0% |
| !! | AAACATTTTTCTATCTCTCT+TGG | - | 8684:3420-3439 | MS.gene060314:intergenic | 25.0% |
| !! | GTTATTGATTAAAATGAGAG+AGG | - | 8684:3669-3688 | MS.gene060314:intergenic | 25.0% |
| !!! | AACAATTTTATTTAGGACAC+CGG | + | 8684:3527-3546 | MS.gene060314:intron | 25.0% |
| !!! | AGAAATTTTGATCAATTAGG+GGG | - | 8684:3359-3378 | MS.gene060314:intergenic | 25.0% |
| !!! | GAGAAATTTTGATCAATTAG+GGG | - | 8684:3360-3379 | MS.gene060314:intergenic | 25.0% |
| ACATAAGGAAATCAAGAGAT+TGG | - | 8684:2774-2793 | MS.gene060314:intergenic | 30.0% | |
| CAAAAGAAAAGGAGAAAAAC+AGG | - | 8684:2672-2691 | MS.gene060314:intergenic | 30.0% | |
| CATGTTCCATCATCTTTAAT+TGG | + | 8684:3903-3922 | MS.gene060314:intron | 30.0% | |
| GATCAAAGGTAAATTTGTGA+TGG | - | 8684:4061-4080 | MS.gene060314:intergenic | 30.0% | |
| TATGTATGTCTGTGTTTAGA+AGG | + | 8684:2862-2881 | MS.gene060314:intron | 30.0% | |
| TTCATCAGCTGAATTTCAAA+AGG | + | 8684:2628-2647 | MS.gene060314:CDS | 30.0% | |
| TTGATTAAAATGAGAGAGGA+AGG | - | 8684:3665-3684 | MS.gene060314:intergenic | 30.0% | |
| ! | AAGGTTTTTGAATCATGCTT+AGG | - | 8684:3646-3665 | MS.gene060314:intergenic | 30.0% |
| ! | CAATAACTTAGGAATCCTTT+TGG | + | 8684:3682-3701 | MS.gene060314:intron | 30.0% |
| ! | GCTTTAAATTGGACTTTGTA+GGG | + | 8684:4120-4139 | MS.gene060314:intron | 30.0% |
| ! | TTAACTCATTTTCAGCGATT+GGG | + | 8684:2745-2764 | MS.gene060314:intron | 30.0% |
| !! | AGCTTTAAATTGGACTTTGT+AGG | + | 8684:4119-4138 | MS.gene060314:intron | 30.0% |
| !! | CAATTAAAGATGATGGAACA+TGG | - | 8684:3905-3924 | MS.gene060314:intergenic | 30.0% |
| !! | GAGAAGAGTATTATTTGGAT+TGG | - | 8684:2958-2977 | MS.gene060314:intergenic | 30.0% |
| !! | GATATGAAGCAGCTTTAAAT+TGG | + | 8684:4109-4128 | MS.gene060314:intron | 30.0% |
| AAACAGGACAGAATGAAAAC+TGG | - | 8684:2656-2675 | None:intergenic | 35.0% | |
| ACTTAGACGAGTATCACATA+AGG | - | 8684:2789-2808 | MS.gene060314:intergenic | 35.0% | |
| GGGAAGTAATAATGTTCAGT+GGG | + | 8684:4154-4173 | MS.gene060314:CDS | 35.0% | |
| GTTCAGATGATTGCTACAAT+GGG | + | 8684:2603-2622 | MS.gene060314:intron | 35.0% | |
| TAAATTGGACTTTGTAGGGA+CGG | + | 8684:4124-4143 | MS.gene060314:intron | 35.0% | |
| TAATATATGTGTAGCGGTGA+TGG | - | 8684:3162-3181 | MS.gene060314:intergenic | 35.0% | |
| TGGCATTCATGAAAGACAAA+AGG | - | 8684:2987-3006 | MS.gene060314:intergenic | 35.0% | |
| TGTTCAGATGATTGCTACAA+TGG | + | 8684:2602-2621 | MS.gene060314:intron | 35.0% | |
| TTTGCTAAGCTGGAAATACA+TGG | - | 8684:2706-2725 | MS.gene060314:intergenic | 35.0% | |
| ! | CTTAACTCATTTTCAGCGAT+TGG | + | 8684:2744-2763 | MS.gene060314:intron | 35.0% |
| ! | TCCAGCTTAGCAAATTTTCA+CGG | + | 8684:2712-2731 | MS.gene060314:intron | 35.0% |
| ! | TGACTTTTCTGACTATTCAC+TGG | + | 8684:3950-3969 | MS.gene060314:intron | 35.0% |
| !! | GAAAAGGTGGTTCAAGTTTT+TGG | - | 8684:3871-3890 | MS.gene060314:intergenic | 35.0% |
| !! | TTCAAGTTTTTGGAGGATGA+TGG | - | 8684:3861-3880 | MS.gene060314:intergenic | 35.0% |
| AAATTGGACTTTGTAGGGAC+GGG | + | 8684:4125-4144 | MS.gene060314:intron | 40.0% | |
| AGCCAGCAAAGTAATTGCAA+TGG | - | 8684:3007-3026 | MS.gene060314:intergenic | 40.0% | |
| ATACACGCAGAATGACCAAA+AGG | - | 8684:3700-3719 | MS.gene060314:intergenic | 40.0% | |
| ATGGAACATGGAACATACTC+TGG | - | 8684:3893-3912 | MS.gene060314:intergenic | 40.0% | |
| ATGGCTCGTATGAGTGTTAT+AGG | + | 8684:3465-3484 | MS.gene060314:intron | 40.0% | |
| CGTATGAGTGTTATAGGTGT+CGG | + | 8684:3471-3490 | MS.gene060314:intron | 40.0% | |
| CTTAGGCAAGTTATGAAGCA+CGG | - | 8684:3629-3648 | MS.gene060314:intergenic | 40.0% | |
| GGAAAAGATGCACATTGATC+AGG | + | 8684:2502-2521 | MS.gene060314:CDS | 40.0% | |
| GGGGAAGTAATAATGTTCAG+TGG | + | 8684:4153-4172 | MS.gene060314:CDS | 40.0% | |
| TGAGAGACATGAACCTGTAA+TGG | - | 8684:2831-2850 | MS.gene060314:intergenic | 40.0% | |
| TGCCATTGCAATTACTTTGC+TGG | + | 8684:3002-3021 | MS.gene060314:intron | 40.0% | |
| TTTGTCTGAGTTGTCTGACA+TGG | + | 8684:3446-3465 | MS.gene060314:intron | 40.0% | |
| ! | CAGCTTAGCAAATTTTCACG+GGG | + | 8684:2714-2733 | MS.gene060314:intron | 40.0% |
| ! | CATGGAACATACTCTGGAAA+AGG | - | 8684:3887-3906 | MS.gene060314:intergenic | 40.0% |
| ! | CCAGCTTAGCAAATTTTCAC+GGG | + | 8684:2713-2732 | MS.gene060314:intron | 40.0% |
| !! | AAGGTGGTTCAAGTTTTTGG+AGG | - | 8684:3868-3887 | MS.gene060314:intergenic | 40.0% |
| !!! | TTTTTGGAGGATGATGGATG+AGG | - | 8684:3855-3874 | MS.gene060314:intergenic | 40.0% |
| AATTGGACTTTGTAGGGACG+GGG | + | 8684:4126-4145 | MS.gene060314:intron | 45.0% | |
| CGCACAATTCAGATCAGAGA+GGG | - | 8684:3104-3123 | MS.gene060314:intergenic | 45.0% | |
| GACATGCTGTCATCCATTAC+AGG | + | 8684:2815-2834 | MS.gene060314:CDS | 45.0% | |
| TTACTTCGACACTCCTAGAC+CGG | - | 8684:3500-3519 | MS.gene060314:intergenic | 45.0% | |
| ! | GGAACATACTCTGGAAAAGG+TGG | - | 8684:3884-3903 | MS.gene060314:intergenic | 45.0% |
| !! | CCCGTGAAAATTTGCTAAGC+TGG | - | 8684:2716-2735 | MS.gene060314:intergenic | 45.0% |
| !! | GCGCTGTTTTCAATTAGCAG+TGG | + | 8684:3972-3991 | MS.gene060314:intron | 45.0% |
| !! | GTGTTATAGGTGTCGGATAC+CGG | + | 8684:3478-3497 | MS.gene060314:intron | 45.0% |
| !! | TATATTAATTTATTTATAGA+CGG | + | 8684:3175-3194 | MS.gene060314:intron | 5.0% |
| ACGTGTCGGACAACTAAGAC+CGG | - | 8684:3549-3568 | MS.gene060314:intergenic | 50.0% | |
| ACGTGTCGTACGAGTGTCAT+GGG | + | 8684:3565-3584 | MS.gene060314:intron | 50.0% | |
| ATTGGACTTTGTAGGGACGG+GGG | + | 8684:4127-4146 | MS.gene060314:intron | 50.0% | |
| GCGCACAATTCAGATCAGAG+AGG | - | 8684:3105-3124 | MS.gene060314:intergenic | 50.0% | |
| TATGAAGCACGGACACCTCT+AGG | - | 8684:3618-3637 | MS.gene060314:intergenic | 50.0% | |
| TCAGATCAGAGAGGGTGAGT+AGG | - | 8684:3096-3115 | MS.gene060314:intergenic | 50.0% | |
| ACTTTGTAGGGACGGGGGTT+GGG | + | 8684:4132-4151 | MS.gene060314:intron | 55.0% | |
| CACGTGTCGTACGAGTGTCA+TGG | + | 8684:3564-3583 | MS.gene060314:intron | 55.0% | |
| GACACTCGTACGACACGTGT+CGG | - | 8684:3563-3582 | MS.gene060314:intergenic | 55.0% | |
| TTGCTTCAACGAGTCACGCG+TGG | + | 8684:3809-3828 | MS.gene060314:CDS | 55.0% | |
| ! | TACGAGTGTCATGGGAGTGC+CGG | + | 8684:3573-3592 | MS.gene060314:intron | 55.0% |
| !! | CTCTAGGACTAGACGTGTCG+CGG | - | 8684:3602-3621 | MS.gene060314:intergenic | 55.0% |
| !! | TAGGTGTCGGATACCGGTCT+AGG | + | 8684:3484-3503 | MS.gene060314:intron | 55.0% |
| ACTAGACGTGTCGCGGTGTC+CGG | - | 8684:3595-3614 | MS.gene060314:intergenic | 60.0% | |
| CGCGACACGTCTAGTCCTAG+AGG | + | 8684:3600-3619 | MS.gene060314:intron | 60.0% | |
| CTTTGTAGGGACGGGGGTTG+GGG | + | 8684:4133-4152 | MS.gene060314:intron | 60.0% | |
| GACTTTGTAGGGACGGGGGT+TGG | + | 8684:4131-4150 | MS.gene060314:intron | 60.0% | |
| TTTGTAGGGACGGGGGTTGG+GGG | + | 8684:4134-4153 | MS.gene060314:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8684 | gene | 2500 | 4181 | 2500 | ID=MS.gene060314 |
| 8684 | mRNA | 2500 | 4181 | 2500 | ID=MS.gene060314.t1;Parent=MS.gene060314 |
| 8684 | exon | 2500 | 2523 | 2500 | ID=MS.gene060314.t1.exon1;Parent=MS.gene060314.t1 |
| 8684 | CDS | 2500 | 2523 | 2500 | ID=cds.MS.gene060314.t1;Parent=MS.gene060314.t1 |
| 8684 | exon | 2609 | 2649 | 2609 | ID=MS.gene060314.t1.exon2;Parent=MS.gene060314.t1 |
| 8684 | CDS | 2609 | 2649 | 2609 | ID=cds.MS.gene060314.t1;Parent=MS.gene060314.t1 |
| 8684 | exon | 2760 | 2836 | 2760 | ID=MS.gene060314.t1.exon3;Parent=MS.gene060314.t1 |
| 8684 | CDS | 2760 | 2836 | 2760 | ID=cds.MS.gene060314.t1;Parent=MS.gene060314.t1 |
| 8684 | exon | 3799 | 3894 | 3799 | ID=MS.gene060314.t1.exon4;Parent=MS.gene060314.t1 |
| 8684 | CDS | 3799 | 3894 | 3799 | ID=cds.MS.gene060314.t1;Parent=MS.gene060314.t1 |
| 8684 | exon | 4141 | 4181 | 4141 | ID=MS.gene060314.t1.exon5;Parent=MS.gene060314.t1 |
| 8684 | CDS | 4141 | 4181 | 4141 | ID=cds.MS.gene060314.t1;Parent=MS.gene060314.t1 |
| Gene Sequence |
| Protein sequence |
