Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80266.t1 | XP_003613224.1 | 88 | 108 | 4 | 1 | 61 | 159 | 61 | 168 | 2.10E-41 | 178.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80266.t1 | G7K1C9 | 88.0 | 108 | 4 | 1 | 61 | 159 | 61 | 168 | 1.5e-41 | 178.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene80266 | 0.888607 | 5.08E-73 | -1.69E-46 |
| MS.gene050310 | MS.gene80266 | 0.828587 | 8.27E-55 | -1.69E-46 |
| MS.gene051018 | MS.gene80266 | 0.831423 | 1.68E-55 | -1.69E-46 |
| MS.gene051067 | MS.gene80266 | 0.813862 | 2.06E-51 | -1.69E-46 |
| MS.gene051478 | MS.gene80266 | 0.859513 | 3.93E-63 | -1.69E-46 |
| MS.gene05157 | MS.gene80266 | 0.809118 | 2.22E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene80266 | 0.814531 | 1.47E-51 | -1.69E-46 |
| MS.gene051781 | MS.gene80266 | 0.800878 | 1.17E-48 | -1.69E-46 |
| MS.gene052155 | MS.gene80266 | -0.824695 | 7.02E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene80266 | 0.810261 | 1.26E-50 | -1.69E-46 |
| MS.gene053122 | MS.gene80266 | 0.806564 | 7.74E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene80266 | 0.860229 | 2.39E-63 | -1.69E-46 |
| MS.gene053536 | MS.gene80266 | 0.846947 | 1.58E-59 | -1.69E-46 |
| MS.gene053538 | MS.gene80266 | 0.849778 | 2.60E-60 | -1.69E-46 |
| MS.gene053581 | MS.gene80266 | 0.811448 | 6.96E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene80266 | 0.854243 | 1.40E-61 | -1.69E-46 |
| MS.gene055465 | MS.gene80266 | 0.809328 | 2.00E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene80266 | 0.811542 | 6.65E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene80266 | 0.846375 | 2.26E-59 | -1.69E-46 |
| MS.gene055580 | MS.gene80266 | -0.825394 | 4.80E-54 | -1.69E-46 |
| MS.gene056169 | MS.gene80266 | 0.814628 | 1.40E-51 | -1.69E-46 |
| MS.gene056228 | MS.gene80266 | 0.818863 | 1.57E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene80266 | 0.808806 | 2.58E-50 | -1.69E-46 |
| MS.gene057082 | MS.gene80266 | 0.832102 | 1.14E-55 | -1.69E-46 |
| MS.gene058339 | MS.gene80266 | 0.800749 | 1.25E-48 | -1.69E-46 |
| MS.gene058909 | MS.gene80266 | 0.838074 | 3.57E-57 | -1.69E-46 |
| MS.gene058912 | MS.gene80266 | 0.836046 | 1.18E-56 | -1.69E-46 |
| MS.gene05894 | MS.gene80266 | 0.852543 | 4.31E-61 | -1.69E-46 |
| MS.gene059300 | MS.gene80266 | 0.803736 | 3.02E-49 | -1.69E-46 |
| MS.gene059424 | MS.gene80266 | 0.83317 | 6.22E-56 | -1.69E-46 |
| MS.gene059425 | MS.gene80266 | 0.802683 | 4.99E-49 | -1.69E-46 |
| MS.gene059778 | MS.gene80266 | 0.80627 | 8.93E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene80266 | 0.802059 | 6.72E-49 | -1.69E-46 |
| MS.gene06057 | MS.gene80266 | 0.806759 | 7.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80266.t1 | MTR_5g034200 | 94.643 | 168 | 0 | 1 | 1 | 159 | 1 | 168 | 8.96e-113 | 317 |
| MS.gene80266.t1 | MTR_8g064760 | 38.365 | 159 | 63 | 6 | 20 | 158 | 18 | 161 | 2.45e-21 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80266.t1 | AT1G23530 | 34.054 | 185 | 82 | 6 | 11 | 158 | 7 | 188 | 1.03e-16 | 73.9 |
Find 33 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTGATATTGAATT+TGG | 0.135097 | 5.4:+24176970 | MS.gene80266:CDS |
| AATTCAATATCAACTTCTTT+TGG | 0.244618 | 5.4:-24176967 | None:intergenic |
| CATTCATCAAATGGATCATT+TGG | 0.283971 | 5.4:+24176796 | MS.gene80266:CDS |
| CCTCCATGTCCATTATTCAT+AGG | 0.373664 | 5.4:-24177021 | None:intergenic |
| GCTTCTAAGCCTATGAATAA+TGG | 0.377824 | 5.4:+24177012 | MS.gene80266:CDS |
| CTGTTGCTTGTTGTCTTGGA+AGG | 0.386601 | 5.4:+24176860 | MS.gene80266:CDS |
| TCTGCTGTTGCTTGTTGTCT+TGG | 0.387132 | 5.4:+24176856 | MS.gene80266:CDS |
| GATGAATGGTGGTCAGGTTC+AGG | 0.392297 | 5.4:-24176781 | None:intergenic |
| TTTCAAAACTCTTCATATCA+TGG | 0.397060 | 5.4:-24177104 | None:intergenic |
| TCATGGTGACCTTGAGCAAC+TGG | 0.407489 | 5.4:-24177087 | None:intergenic |
| TGTTGTTGTTGTTGCTGCAT+TGG | 0.421046 | 5.4:-24176709 | None:intergenic |
| ATTCATCAAATGGATCATTT+GGG | 0.438895 | 5.4:+24176797 | MS.gene80266:CDS |
| AGTATAGGCCAGGTGCATAA+TGG | 0.443427 | 5.4:+24177151 | MS.gene80266:CDS |
| CCTGACCACCATTCATCAAA+TGG | 0.451998 | 5.4:+24176787 | MS.gene80266:CDS |
| CAGGTTCAGGTGGTGCATAC+TGG | 0.460578 | 5.4:-24176768 | None:intergenic |
| CCATTTGATGAATGGTGGTC+AGG | 0.470979 | 5.4:-24176787 | None:intergenic |
| GTGGTTGGATAAACTTGAAC+TGG | 0.498993 | 5.4:-24176736 | None:intergenic |
| AGCAGAAATTACTATTATGA+TGG | 0.518824 | 5.4:-24176839 | None:intergenic |
| GTCCTCCTCAAGGAAAGTAT+AGG | 0.536696 | 5.4:+24177136 | MS.gene80266:CDS |
| CAAATGATCCATTTGATGAA+TGG | 0.542377 | 5.4:-24176795 | None:intergenic |
| TACTGGTATGTGACAGTGGT+TGG | 0.552488 | 5.4:-24176751 | None:intergenic |
| GCTTAACAGGTTTCTGACTG+TGG | 0.553296 | 5.4:-24176912 | None:intergenic |
| ATAAAGCTTGGTCCTCCTCA+AGG | 0.553613 | 5.4:+24177126 | MS.gene80266:CDS |
| TTGTAACTCCATTATGCACC+TGG | 0.558632 | 5.4:-24177159 | None:intergenic |
| CCTCAAGGAAAGTATAGGCC+AGG | 0.560026 | 5.4:+24177141 | MS.gene80266:CDS |
| GGCCTATACTTTCCTTGAGG+AGG | 0.568497 | 5.4:-24177138 | None:intergenic |
| CCTGGCCTATACTTTCCTTG+AGG | 0.589930 | 5.4:-24177141 | None:intergenic |
| GTACAGAAGCCAGTTGCTCA+AGG | 0.601141 | 5.4:+24177078 | MS.gene80266:CDS |
| GAATGGTGGTCAGGTTCAGG+TGG | 0.612964 | 5.4:-24176778 | None:intergenic |
| AAGCCTATGAATAATGGACA+TGG | 0.624027 | 5.4:+24177018 | MS.gene80266:CDS |
| TGCATACTGGTATGTGACAG+TGG | 0.646713 | 5.4:-24176755 | None:intergenic |
| CCTATGAATAATGGACATGG+AGG | 0.659770 | 5.4:+24177021 | MS.gene80266:CDS |
| ATGATCCATTTGATGAATGG+TGG | 0.679103 | 5.4:-24176792 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAGTTGATATTGAATT+TGG | + | chr5.4:24176970-24176989 | MS.gene80266:CDS | 20.0% |
| !!! | AATTCAATATCAACTTCTTT+TGG | - | chr5.4:24176970-24176989 | None:intergenic | 20.0% |
| !!! | TTTTCAAAAAATAATGGACA+TGG | + | chr5.4:24177045-24177064 | MS.gene80266:CDS | 20.0% |
| ! | AGCAGAAATTACTATTATGA+TGG | - | chr5.4:24176842-24176861 | None:intergenic | 25.0% |
| ! | ATTCATCAAATGGATCATTT+GGG | + | chr5.4:24176797-24176816 | MS.gene80266:CDS | 25.0% |
| ! | TTTCAAAACTCTTCATATCA+TGG | - | chr5.4:24177107-24177126 | None:intergenic | 25.0% |
| !!! | AAGAGTTTTGAAATAAAGCT+TGG | + | chr5.4:24177114-24177133 | MS.gene80266:CDS | 25.0% |
| CAAATGATCCATTTGATGAA+TGG | - | chr5.4:24176798-24176817 | None:intergenic | 30.0% | |
| CATTCATCAAATGGATCATT+TGG | + | chr5.4:24176796-24176815 | MS.gene80266:CDS | 30.0% | |
| ! | GGAGGCTTTTCAAAAAATAA+TGG | + | chr5.4:24177039-24177058 | MS.gene80266:CDS | 30.0% |
| !! | TCAAAAAATAATGGACATGG+AGG | + | chr5.4:24177048-24177067 | MS.gene80266:CDS | 30.0% |
| ATGATCCATTTGATGAATGG+TGG | - | chr5.4:24176795-24176814 | None:intergenic | 35.0% | |
| GCTTCTAAGCCTATGAATAA+TGG | + | chr5.4:24177012-24177031 | MS.gene80266:CDS | 35.0% | |
| !! | AAGCCTATGAATAATGGACA+TGG | + | chr5.4:24177018-24177037 | MS.gene80266:CDS | 35.0% |
| CCTCCATGTCCATTATTCAT+AGG | - | chr5.4:24177024-24177043 | None:intergenic | 40.0% | |
| GTGGTTGGATAAACTTGAAC+TGG | - | chr5.4:24176739-24176758 | None:intergenic | 40.0% | |
| ! | TGTTGTTGTTGTTGCTGCAT+TGG | - | chr5.4:24176712-24176731 | None:intergenic | 40.0% |
| !! | CCTATGAATAATGGACATGG+AGG | + | chr5.4:24177021-24177040 | MS.gene80266:CDS | 40.0% |
| !!! | TGCTGCTTTTGATGCTTAAC+AGG | - | chr5.4:24176928-24176947 | None:intergenic | 40.0% |
| ATAAAGCTTGGTCCTCCTCA+AGG | + | chr5.4:24177126-24177145 | MS.gene80266:CDS | 45.0% | |
| CCATTTGATGAATGGTGGTC+AGG | - | chr5.4:24176790-24176809 | None:intergenic | 45.0% | |
| CCTGACCACCATTCATCAAA+TGG | + | chr5.4:24176787-24176806 | MS.gene80266:CDS | 45.0% | |
| GCTTAACAGGTTTCTGACTG+TGG | - | chr5.4:24176915-24176934 | None:intergenic | 45.0% | |
| TACTGGTATGTGACAGTGGT+TGG | - | chr5.4:24176754-24176773 | None:intergenic | 45.0% | |
| TGCATACTGGTATGTGACAG+TGG | - | chr5.4:24176758-24176777 | None:intergenic | 45.0% | |
| ! | CTTCTTTTGGACGTGAGTGT+TGG | - | chr5.4:24176957-24176976 | None:intergenic | 45.0% |
| ! | GTCCTCCTCAAGGAAAGTAT+AGG | + | chr5.4:24177136-24177155 | MS.gene80266:CDS | 45.0% |
| !! | CTGTTGCTTGTTGTCTTGGA+AGG | + | chr5.4:24176860-24176879 | MS.gene80266:CDS | 45.0% |
| !! | TCTGCTGTTGCTTGTTGTCT+TGG | + | chr5.4:24176856-24176875 | MS.gene80266:CDS | 45.0% |
| CCTGGCCTATACTTTCCTTG+AGG | - | chr5.4:24177144-24177163 | None:intergenic | 50.0% | |
| GATGAATGGTGGTCAGGTTC+AGG | - | chr5.4:24176784-24176803 | None:intergenic | 50.0% | |
| GGCCTATACTTTCCTTGAGG+AGG | - | chr5.4:24177141-24177160 | None:intergenic | 50.0% | |
| GTACAGAAGCCAGTTGCTCA+AGG | + | chr5.4:24177078-24177097 | MS.gene80266:CDS | 50.0% | |
| TCATGGTGACCTTGAGCAAC+TGG | - | chr5.4:24177090-24177109 | None:intergenic | 50.0% | |
| ! | CCTCAAGGAAAGTATAGGCC+AGG | + | chr5.4:24177141-24177160 | MS.gene80266:CDS | 50.0% |
| CAGGTTCAGGTGGTGCATAC+TGG | - | chr5.4:24176771-24176790 | None:intergenic | 55.0% | |
| GAATGGTGGTCAGGTTCAGG+TGG | - | chr5.4:24176781-24176800 | None:intergenic | 55.0% | |
| ! | TGTGGCTTTTGCCGTTGCCG+CGG | - | chr5.4:24176897-24176916 | None:intergenic | 60.0% |
| ! | GGAAGGTTTTGCAGCCGCCG+CGG | + | chr5.4:24176877-24176896 | MS.gene80266:CDS | 65.0% |
| ! | TTTTGCAGCCGCCGCGGCAA+CGG | + | chr5.4:24176883-24176902 | MS.gene80266:CDS | 65.0% |
| ! | GGCTTTTGCCGTTGCCGCGG+CGG | - | chr5.4:24176894-24176913 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 24176691 | 24177170 | 24176691 | ID=MS.gene80266 |
| chr5.4 | mRNA | 24176691 | 24177170 | 24176691 | ID=MS.gene80266.t1;Parent=MS.gene80266 |
| chr5.4 | exon | 24176691 | 24177170 | 24176691 | ID=MS.gene80266.t1.exon1;Parent=MS.gene80266.t1 |
| chr5.4 | CDS | 24176691 | 24177170 | 24176691 | ID=cds.MS.gene80266.t1;Parent=MS.gene80266.t1 |
| Gene Sequence |
| Protein sequence |
