Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81239.t1 | KHN43942.1 | 93.5 | 93 | 5 | 1 | 1 | 92 | 1 | 93 | 2.90E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81239.t1 | A0A0B2SHY7 | 93.5 | 93 | 5 | 1 | 1 | 92 | 1 | 93 | 2.1e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene81239 | 0.804579 | 2.02E-49 | -1.69E-46 |
| MS.gene049861 | MS.gene81239 | 0.81807 | 2.37E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene81239 | 0.806136 | 9.53E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene81239 | 0.813706 | 2.23E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene81239 | 0.810189 | 1.30E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene81239 | 0.826701 | 2.35E-54 | -1.69E-46 |
| MS.gene05517 | MS.gene81239 | 0.800044 | 1.73E-48 | -1.69E-46 |
| MS.gene056849 | MS.gene81239 | 0.821768 | 3.39E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene81239 | 0.827993 | 1.15E-54 | -1.69E-46 |
| MS.gene057102 | MS.gene81239 | 0.80774 | 4.36E-50 | -1.69E-46 |
| MS.gene057108 | MS.gene81239 | 0.84037 | 9.06E-58 | -1.69E-46 |
| MS.gene057109 | MS.gene81239 | 0.831754 | 1.40E-55 | -1.69E-46 |
| MS.gene057110 | MS.gene81239 | 0.837258 | 5.78E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene81239 | 0.802106 | 6.57E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene81239 | 0.838038 | 3.65E-57 | -1.69E-46 |
| MS.gene058159 | MS.gene81239 | 0.816982 | 4.18E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene81239 | 0.806537 | 7.84E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene81239 | 0.801882 | 7.30E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene81239 | 0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene059830 | MS.gene81239 | 0.811891 | 5.58E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene81239 | 0.803159 | 3.98E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene81239 | 0.832451 | 9.38E-56 | -1.69E-46 |
| MS.gene060586 | MS.gene81239 | 0.805534 | 1.27E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene81239 | 0.824037 | 1.00E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene81239 | 0.830374 | 3.04E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81239.t1 | MTR_5g097240 | 98.780 | 82 | 1 | 0 | 1 | 82 | 1 | 82 | 1.23e-57 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81239.t1 | AT3G07760 | 85.366 | 82 | 12 | 0 | 1 | 82 | 1 | 82 | 6.91e-48 | 149 |
| MS.gene81239.t1 | AT3G07760 | 85.366 | 82 | 12 | 0 | 1 | 82 | 1 | 82 | 6.91e-48 | 149 |
| MS.gene81239.t1 | AT3G07760 | 85.366 | 82 | 12 | 0 | 1 | 82 | 1 | 82 | 6.91e-48 | 149 |
| MS.gene81239.t1 | AT3G07760 | 85.366 | 82 | 12 | 0 | 1 | 82 | 16 | 97 | 1.59e-47 | 148 |
| MS.gene81239.t1 | AT3G07760 | 87.879 | 66 | 8 | 0 | 17 | 82 | 4 | 69 | 1.89e-37 | 122 |
| MS.gene81239.t1 | AT3G07760 | 87.879 | 66 | 8 | 0 | 17 | 82 | 4 | 69 | 1.89e-37 | 122 |
| MS.gene81239.t1 | AT3G07760 | 89.062 | 64 | 7 | 0 | 19 | 82 | 2 | 65 | 1.18e-36 | 119 |
| MS.gene81239.t1 | AT3G07760 | 89.062 | 64 | 7 | 0 | 19 | 82 | 2 | 65 | 1.18e-36 | 119 |
| MS.gene81239.t1 | AT3G07760 | 86.364 | 66 | 9 | 0 | 17 | 82 | 33 | 98 | 1.97e-36 | 120 |
| MS.gene81239.t1 | AT3G07760 | 86.364 | 66 | 9 | 0 | 17 | 82 | 33 | 98 | 1.97e-36 | 120 |
| MS.gene81239.t1 | AT3G07760 | 86.364 | 66 | 9 | 0 | 17 | 82 | 33 | 98 | 1.97e-36 | 120 |
Find 22 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAAGGAAAACGGTGTTAA+TGG | 0.231279 | 5.2:+81517288 | MS.gene81239:CDS |
| AGCCTCTTGATTTCTTCATC+TGG | 0.317399 | 5.2:+81515668 | MS.gene81239:CDS |
| AAGGAATTGAGACGGATTAA+AGG | 0.333350 | 5.2:+81517408 | MS.gene81239:CDS |
| CGGTGTTAATGGAGAATACT+TGG | 0.411738 | 5.2:+81517299 | MS.gene81239:CDS |
| ATGAAGAAATCAAGAGGCTC+AGG | 0.506751 | 5.2:-81515664 | None:intergenic |
| CTATAAATCTTGGTAGCTAT+CGG | 0.528784 | 5.2:+81517259 | MS.gene81239:CDS |
| TTTCTTCATCTGGACTGTTG+AGG | 0.537401 | 5.2:+81515678 | MS.gene81239:CDS |
| TGGTTAGAGACTATAAATCT+TGG | 0.543949 | 5.2:+81517249 | MS.gene81239:CDS |
| TAAGACGCTGTCACATGAAG+TGG | 0.544823 | 5.2:+81517364 | MS.gene81239:CDS |
| TTACTGTTCAAGAGAGAGAG+AGG | 0.552572 | 5.2:-81517435 | None:intergenic |
| TACTGTTCAAGAGAGAGAGA+GGG | 0.559423 | 5.2:-81517434 | None:intergenic |
| GTTAATGGAGAATACTTGGA+AGG | 0.563026 | 5.2:+81517303 | MS.gene81239:CDS |
| TTGATTTGTGAAATGAACAA+AGG | 0.570306 | 5.2:+81515634 | None:intergenic |
| AAGACGCTGTCACATGAAGT+GGG | 0.581523 | 5.2:+81517365 | MS.gene81239:CDS |
| TTAATGGAGAATACTTGGAA+GGG | 0.582724 | 5.2:+81517304 | MS.gene81239:CDS |
| CCGTCTCAATTCCTTGCACA+AGG | 0.598225 | 5.2:-81517400 | None:intergenic |
| AGACTTCATCACCTTGTGCA+AGG | 0.598256 | 5.2:+81517389 | MS.gene81239:CDS |
| GTCCAGATGAAGAAATCAAG+AGG | 0.605092 | 5.2:-81515670 | None:intergenic |
| CCTTGTGCAAGGAATTGAGA+CGG | 0.632068 | 5.2:+81517400 | MS.gene81239:CDS |
| TTTATCAGGATGTTGGCATG+TGG | 0.634856 | 5.2:+81517229 | MS.gene81239:intron |
| GACAGCGTCTTATGAATCGA+AGG | 0.642041 | 5.2:-81517353 | None:intergenic |
| AGACGCTGTCACATGAAGTG+GGG | 0.697436 | 5.2:+81517366 | MS.gene81239:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAACTATAGTTTAATAA+AGG | - | chr5.2:81516521-81516540 | None:intergenic | 15.0% |
| !!! | GTTGAATACAATATTTTTAA+TGG | + | chr5.2:81516392-81516411 | MS.gene81239:intron | 15.0% |
| !!! | TATTGTATTCAACATTTTAA+CGG | - | chr5.2:81516385-81516404 | None:intergenic | 15.0% |
| !! | AAACTATAGTTTAATAAAGG+CGG | - | chr5.2:81516518-81516537 | None:intergenic | 20.0% |
| !! | AACAAAGACAGATAATAAAT+AGG | - | chr5.2:81516843-81516862 | None:intergenic | 20.0% |
| !! | CTGATAAAACAAACAAAAAA+TGG | - | chr5.2:81517217-81517236 | None:intergenic | 20.0% |
| !!! | AAATTATCATTTTAAAACCG+AGG | + | chr5.2:81516616-81516635 | MS.gene81239:intron | 20.0% |
| !!! | CATTTTTTGTTTGTTTTATC+AGG | + | chr5.2:81517215-81517234 | MS.gene81239:intron | 20.0% |
| !!! | GAATACAATATTTTTAATGG+TGG | + | chr5.2:81516395-81516414 | MS.gene81239:intron | 20.0% |
| ! | AGATATTTGCAATTATGTCT+TGG | + | chr5.2:81516793-81516812 | MS.gene81239:intron | 25.0% |
| ! | CAAAACTTTCTACTTCAAAT+TGG | - | chr5.2:81516085-81516104 | None:intergenic | 25.0% |
| ! | TAATAAGACCTATGTTAACA+TGG | - | chr5.2:81515999-81516018 | None:intergenic | 25.0% |
| !! | AAGTTAAACTGTTTTCGTAT+AGG | - | chr5.2:81516246-81516265 | None:intergenic | 25.0% |
| !!! | AATATTTTTAATGGTGGACT+TGG | + | chr5.2:81516401-81516420 | MS.gene81239:intron | 25.0% |
| !!! | AGTATAATTTTGTTGTGTGT+TGG | + | chr5.2:81515960-81515979 | MS.gene81239:intron | 25.0% |
| !!! | CAATTTGAAGTAGAAAGTTT+TGG | + | chr5.2:81516083-81516102 | MS.gene81239:intron | 25.0% |
| !!! | TTTGAGTTGAATTTACTGTA+TGG | + | chr5.2:81515837-81515856 | MS.gene81239:intron | 25.0% |
| AGAGAAAATAATGTCAACCT+CGG | - | chr5.2:81516636-81516655 | None:intergenic | 30.0% | |
| ATTAAGCTGTTTATCCAAAC+AGG | + | chr5.2:81516351-81516370 | MS.gene81239:intron | 30.0% | |
| CATTTAGATTCCGTTTGATA+TGG | - | chr5.2:81516950-81516969 | None:intergenic | 30.0% | |
| GATATCTACCATGTTAACAT+AGG | + | chr5.2:81515988-81516007 | MS.gene81239:intron | 30.0% | |
| TAAAAACATAGACAACTGCT+AGG | - | chr5.2:81516031-81516050 | None:intergenic | 30.0% | |
| TAGCAAACTATTTCCCTAAA+TGG | - | chr5.2:81516449-81516468 | None:intergenic | 30.0% | |
| TGGTTAGAGACTATAAATCT+TGG | + | chr5.2:81517249-81517268 | MS.gene81239:CDS | 30.0% | |
| TTAATGGAGAATACTTGGAA+GGG | + | chr5.2:81517304-81517323 | MS.gene81239:CDS | 30.0% | |
| ! | CTATAAATCTTGGTAGCTAT+CGG | + | chr5.2:81517259-81517278 | MS.gene81239:CDS | 30.0% |
| ! | GAATTTACAGAAGTATGGAA+AGG | - | chr5.2:81516059-81516078 | None:intergenic | 30.0% |
| ! | TAGCTATCGGCAAATTTTTA+AGG | + | chr5.2:81517272-81517291 | MS.gene81239:CDS | 30.0% |
| ! | TTTAAGGAAAACGGTGTTAA+TGG | + | chr5.2:81517288-81517307 | MS.gene81239:CDS | 30.0% |
| !! | ATGCATATTGTTAGCTTTAC+TGG | - | chr5.2:81516766-81516785 | None:intergenic | 30.0% |
| !! | ATTCAACATTTTAACGGACA+AGG | - | chr5.2:81516379-81516398 | None:intergenic | 30.0% |
| !! | CATAGGTCTTATTAGCAATT+CGG | + | chr5.2:81516005-81516024 | MS.gene81239:intron | 30.0% |
| !! | CGGCAAATTTTTAAGGAAAA+CGG | + | chr5.2:81517279-81517298 | MS.gene81239:CDS | 30.0% |
| !! | GTCATCTTGTTATTCTTCTT+TGG | + | chr5.2:81517018-81517037 | MS.gene81239:intron | 30.0% |
| !! | TGCATATTGTTAGCTTTACT+GGG | - | chr5.2:81516765-81516784 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTATCCCAATTAA+TGG | + | chr5.2:81516558-81516577 | MS.gene81239:intron | 30.0% |
| !!! | TGTTTGTTTTATCAGGATGT+TGG | + | chr5.2:81517222-81517241 | MS.gene81239:intron | 30.0% |
| AAAGTTGTCCGCCATTAATT+GGG | - | chr5.2:81516572-81516591 | None:intergenic | 35.0% | |
| AAGCTTATGCATGCACTATT+GGG | + | chr5.2:81516472-81516491 | MS.gene81239:intron | 35.0% | |
| GGTCAGAATTTACAGAAGTA+TGG | - | chr5.2:81516064-81516083 | None:intergenic | 35.0% | |
| GTTAATGGAGAATACTTGGA+AGG | + | chr5.2:81517303-81517322 | MS.gene81239:CDS | 35.0% | |
| GTTACTAGAGCCATATCAAA+CGG | + | chr5.2:81516937-81516956 | MS.gene81239:intron | 35.0% | |
| TACTGCTACCTTTGATATGT+TGG | - | chr5.2:81517060-81517079 | None:intergenic | 35.0% | |
| TAGAAGTCACTCTCCATTTA+GGG | + | chr5.2:81516433-81516452 | MS.gene81239:intron | 35.0% | |
| TAGTAACCTACTTAACTGAG+CGG | - | chr5.2:81516924-81516943 | None:intergenic | 35.0% | |
| TATGTGTGCCAACATATCAA+AGG | + | chr5.2:81517049-81517068 | MS.gene81239:intron | 35.0% | |
| !! | AAGGAATTGAGACGGATTAA+AGG | + | chr5.2:81517408-81517427 | MS.gene81239:CDS | 35.0% |
| !! | CATATTGTTAGCTTTACTGG+GGG | - | chr5.2:81516763-81516782 | None:intergenic | 35.0% |
| !! | GCATATTGTTAGCTTTACTG+GGG | - | chr5.2:81516764-81516783 | None:intergenic | 35.0% |
| !! | GGTTTTATCCCAATTAATGG+CGG | + | chr5.2:81516561-81516580 | MS.gene81239:intron | 35.0% |
| !!! | ATTTTTAATGGTGGACTTGG+TGG | + | chr5.2:81516404-81516423 | MS.gene81239:intron | 35.0% |
| !!! | TCTTCGTTTTTTCGGTGATA+TGG | + | chr5.2:81516874-81516893 | MS.gene81239:intron | 35.0% |
| ACCGAAAAAACGAAGACTAC+GGG | - | chr5.2:81516870-81516889 | None:intergenic | 40.0% | |
| AGCCTCTTGATTTCTTCATC+TGG | + | chr5.2:81515668-81515687 | MS.gene81239:CDS | 40.0% | |
| ATGAAGAAATCAAGAGGCTC+AGG | - | chr5.2:81515667-81515686 | None:intergenic | 40.0% | |
| CAAGCTTATGCATGCACTAT+TGG | + | chr5.2:81516471-81516490 | MS.gene81239:intron | 40.0% | |
| CACCGAAAAAACGAAGACTA+CGG | - | chr5.2:81516871-81516890 | None:intergenic | 40.0% | |
| CTGTTTATTCCCGCAATGTA+TGG | + | chr5.2:81516678-81516697 | MS.gene81239:intron | 40.0% | |
| GAAAGTTGTCCGCCATTAAT+TGG | - | chr5.2:81516573-81516592 | None:intergenic | 40.0% | |
| GCTTATCATGTTAATCGCTC+AGG | + | chr5.2:81516315-81516334 | MS.gene81239:intron | 40.0% | |
| GTAGAAGTCACTCTCCATTT+AGG | + | chr5.2:81516432-81516451 | MS.gene81239:intron | 40.0% | |
| GTCCAGATGAAGAAATCAAG+AGG | - | chr5.2:81515673-81515692 | None:intergenic | 40.0% | |
| GTTTGTGATCGCAAGTTATC+TGG | + | chr5.2:81516540-81516559 | MS.gene81239:intron | 40.0% | |
| TACTGTTCAAGAGAGAGAGA+GGG | - | chr5.2:81517437-81517456 | None:intergenic | 40.0% | |
| TTACTGTTCAAGAGAGAGAG+AGG | - | chr5.2:81517438-81517457 | None:intergenic | 40.0% | |
| TTTATCAGGATGTTGGCATG+TGG | + | chr5.2:81517229-81517248 | MS.gene81239:intron | 40.0% | |
| TTTCTTCATCTGGACTGTTG+AGG | + | chr5.2:81515678-81515697 | MS.gene81239:CDS | 40.0% | |
| ! | AAAGGCATGACCATACATTG+CGG | - | chr5.2:81516691-81516710 | None:intergenic | 40.0% |
| ! | AGCATAGAGCATCAACTACA+GGG | - | chr5.2:81517155-81517174 | None:intergenic | 40.0% |
| ! | CGGTGTTAATGGAGAATACT+TGG | + | chr5.2:81517299-81517318 | MS.gene81239:CDS | 40.0% |
| AAAGGCGGTGACGAGATTTA+GGG | - | chr5.2:81516503-81516522 | None:intergenic | 45.0% | |
| AAGACGCTGTCACATGAAGT+GGG | + | chr5.2:81517365-81517384 | MS.gene81239:CDS | 45.0% | |
| AAGGCATGACCATACATTGC+GGG | - | chr5.2:81516690-81516709 | None:intergenic | 45.0% | |
| AGACTTCATCACCTTGTGCA+AGG | + | chr5.2:81517389-81517408 | MS.gene81239:CDS | 45.0% | |
| CCTTGTGCAAGGAATTGAGA+CGG | + | chr5.2:81517400-81517419 | MS.gene81239:CDS | 45.0% | |
| CTTATCCAGGACTGTTGCAT+AGG | + | chr5.2:81517118-81517137 | MS.gene81239:intron | 45.0% | |
| GACAGCGTCTTATGAATCGA+AGG | - | chr5.2:81517356-81517375 | None:intergenic | 45.0% | |
| TAAAGGCGGTGACGAGATTT+AGG | - | chr5.2:81516504-81516523 | None:intergenic | 45.0% | |
| TAAGACGCTGTCACATGAAG+TGG | + | chr5.2:81517364-81517383 | MS.gene81239:CDS | 45.0% | |
| TTGTCACCGCTCAGTTAAGT+AGG | + | chr5.2:81516915-81516934 | MS.gene81239:intron | 45.0% | |
| ! | GAGCATAGAGCATCAACTAC+AGG | - | chr5.2:81517156-81517175 | None:intergenic | 45.0% |
| !! | TGTTAGCTTTACTGGGGGAA+TGG | - | chr5.2:81516758-81516777 | None:intergenic | 45.0% |
| !!! | GCCCGTAGTCTTCGTTTTTT+CGG | + | chr5.2:81516866-81516885 | MS.gene81239:intron | 45.0% |
| AAGGCGGTGACGAGATTTAG+GGG | - | chr5.2:81516502-81516521 | None:intergenic | 50.0% | |
| AGACGCTGTCACATGAAGTG+GGG | + | chr5.2:81517366-81517385 | MS.gene81239:CDS | 50.0% | |
| CCGTCTCAATTCCTTGCACA+AGG | - | chr5.2:81517403-81517422 | None:intergenic | 50.0% | |
| CTAGACCTATGCAACAGTCC+TGG | - | chr5.2:81517126-81517145 | None:intergenic | 50.0% | |
| CTTGCATGTGCTGCAGAGAA+AGG | - | chr5.2:81516709-81516728 | None:intergenic | 50.0% | |
| CTTTCTCTGCAGCACATGCA+AGG | + | chr5.2:81516707-81516726 | MS.gene81239:intron | 50.0% | |
| GTTCAAGCACCTGCTTATCC+AGG | + | chr5.2:81517105-81517124 | MS.gene81239:intron | 50.0% | |
| TGCAACAGTCCTGGATAAGC+AGG | - | chr5.2:81517117-81517136 | None:intergenic | 50.0% | |
| ! | TAACGGACAAGGCACCTGTT+TGG | - | chr5.2:81516368-81516387 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 81515646 | 81517461 | 81515646 | ID=MS.gene81239 |
| chr5.2 | mRNA | 81515646 | 81517461 | 81515646 | ID=MS.gene81239.t1;Parent=MS.gene81239 |
| chr5.2 | exon | 81515646 | 81515699 | 81515646 | ID=MS.gene81239.t1.exon1;Parent=MS.gene81239.t1 |
| chr5.2 | CDS | 81515646 | 81515699 | 81515646 | ID=cds.MS.gene81239.t1;Parent=MS.gene81239.t1 |
| chr5.2 | exon | 81517237 | 81517461 | 81517237 | ID=MS.gene81239.t1.exon2;Parent=MS.gene81239.t1 |
| chr5.2 | CDS | 81517237 | 81517461 | 81517237 | ID=cds.MS.gene81239.t1;Parent=MS.gene81239.t1 |
| Gene Sequence |
| Protein sequence |
