AlfalfaGEDB | MS.gene83292

M. sativa cultivar XinJiangDaYe / MS.gene83292

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83292.t1	XP_013452627.1	84.1	220	30	2	1	218	1	217	1.70E-97	365.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83292.t1	Q41015	51.7	207	91	4	1	200	1	205	4.2e-49	196.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83292.t1	B7FN38	84.1	220	30	2	1	218	1	217	1.2e-97	365.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049251	MS.gene83292	0.814261	1.69E-51	-1.69E-46
MS.gene050338	MS.gene83292	0.822335	2.50E-53	-1.69E-46
MS.gene051439	MS.gene83292	0.804246	2.37E-49	-1.69E-46
MS.gene051914	MS.gene83292	0.838722	2.43E-57	-1.69E-46
MS.gene052143	MS.gene83292	0.809438	1.89E-50	-1.69E-46
MS.gene052308	MS.gene83292	0.818132	2.30E-52	-1.69E-46
MS.gene052367	MS.gene83292	0.81256	3.99E-51	-1.69E-46
MS.gene053173	MS.gene83292	0.873836	1.08E-67	-1.69E-46
MS.gene053348	MS.gene83292	0.822608	2.16E-53	-1.69E-46
MS.gene053416	MS.gene83292	0.808773	2.63E-50	-1.69E-46
MS.gene053417	MS.gene83292	0.805283	1.44E-49	-1.69E-46
MS.gene054551	MS.gene83292	0.871279	7.71E-67	-1.69E-46
MS.gene054593	MS.gene83292	0.844179	8.88E-59	-1.69E-46
MS.gene054633	MS.gene83292	0.847925	8.49E-60	-1.69E-46
MS.gene055251	MS.gene83292	0.800443	1.44E-48	-1.69E-46
MS.gene055308	MS.gene83292	0.825137	5.52E-54	-1.69E-46
MS.gene055578	MS.gene83292	0.801261	9.79E-49	-1.69E-46
MS.gene055616	MS.gene83292	0.817454	3.27E-52	-1.69E-46
MS.gene056041	MS.gene83292	0.82001	8.60E-53	-1.69E-46
MS.gene056266	MS.gene83292	-0.805794	1.12E-49	-1.69E-46
MS.gene05640	MS.gene83292	0.835685	1.45E-56	-1.69E-46
MS.gene056526	MS.gene83292	0.819435	1.16E-52	-1.69E-46
MS.gene05652	MS.gene83292	0.820608	6.27E-53	-1.69E-46
MS.gene056694	MS.gene83292	0.850522	1.61E-60	-1.69E-46
MS.gene056697	MS.gene83292	0.806673	7.34E-50	-1.69E-46
MS.gene057367	MS.gene83292	0.809294	2.03E-50	-1.69E-46
MS.gene057669	MS.gene83292	0.801439	9.00E-49	-1.69E-46
MS.gene058128	MS.gene83292	0.815921	7.22E-52	-1.69E-46
MS.gene058853	MS.gene83292	0.828182	1.04E-54	-1.69E-46
MS.gene05926	MS.gene83292	0.831161	1.95E-55	-1.69E-46
MS.gene059304	MS.gene83292	0.829759	4.30E-55	-1.69E-46
MS.gene059553	MS.gene83292	0.800374	1.49E-48	-1.69E-46
MS.gene06093	MS.gene83292	0.850231	1.94E-60	-1.69E-46
MS.gene061044	MS.gene83292	0.832253	1.05E-55	-1.69E-46
MS.gene061099	MS.gene83292	0.809003	2.35E-50	-1.69E-46
MS.gene061260	MS.gene83292	0.805434	1.34E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83292.t1	MTR_6g066190	84.615	221	27	3	1	218	1	217	9.11e-130	364
MS.gene83292.t1	MTR_6g009650	73.182	220	47	2	1	218	1	210	1.51e-112	320
MS.gene83292.t1	MTR_6g009563	70.320	219	52	3	1	216	1	209	5.85e-106	304
MS.gene83292.t1	MTR_6g066210	66.667	222	64	2	1	218	1	216	1.14e-102	296
MS.gene83292.t1	MTR_6g017225	49.362	235	101	5	1	218	1	234	2.44e-62	199
MS.gene83292.t1	MTR_6g059730	54.808	208	86	4	1	202	1	206	3.86e-61	191
MS.gene83292.t1	MTR_6g059680	53.333	210	90	4	1	202	1	210	2.92e-50	163
MS.gene83292.t1	MTR_1g035770	42.523	214	109	6	1	202	1	212	8.90e-47	154
MS.gene83292.t1	MTR_6g059810	45.833	216	100	8	1	202	1	213	1.10e-42	143
MS.gene83292.t1	MTR_6g065460	44.712	208	103	6	1	200	1	204	1.03e-37	130
MS.gene83292.t1	MTR_6g059650	40.107	187	101	5	22	201	21	203	8.81e-34	120
MS.gene83292.t1	MTR_6g059760	40.191	209	109	9	1	200	1	202	2.18e-28	106
MS.gene83292.t1	MTR_6g065460	40.488	205	92	6	1	200	1	180	6.06e-28	104
MS.gene83292.t1	MTR_6g059530	40.385	208	103	9	1	200	1	195	7.91e-28	105
MS.gene83292.t1	MTR_6g059410	38.537	205	106	8	5	200	1	194	1.64e-25	99.0
MS.gene83292.t1	MTR_6g045403	37.850	214	115	9	1	203	1	207	1.25e-24	97.1
MS.gene83292.t1	MTR_3g463590	37.838	185	98	7	27	201	27	204	2.26e-23	93.6
MS.gene83292.t1	MTR_6g045433	36.957	230	113	11	1	207	1	221	5.15e-23	92.8
MS.gene83292.t1	MTR_6g044810	38.326	227	109	14	1	207	1	216	6.91e-23	92.4
MS.gene83292.t1	MTR_6g044780	38.053	226	111	13	1	207	1	216	1.03e-22	92.4
MS.gene83292.t1	MTR_6g045097	37.778	225	113	13	1	207	1	216	8.52e-22	89.7
MS.gene83292.t1	MTR_1g057110	31.250	208	117	10	1	202	1	188	4.47e-17	76.3
MS.gene83292.t1	MTR_2g073010	31.731	208	114	11	4	200	8	198	2.97e-11	60.8
MS.gene83292.t1	MTR_6g065570	43.662	71	33	1	4	74	9	72	3.53e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83292.t1	AT1G73325	29.545	220	125	11	7	208	4	211	4.18e-11	60.8

Find 49 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAAGTATGGTGAATTTAAC+TGG	0.279016	6.1:+62600185	None:intergenic
CCAGTTAAATTCACCATACT+TGG	0.298543	6.1:-62600185	MS.gene83292:CDS
CATTAGAATCCATACTAAAT+AGG	0.316847	6.1:+62599777	None:intergenic
ACTAGTGATGTTTCCAAGTA+TGG	0.319310	6.1:+62600172	None:intergenic
TGAAACATGGATCTGGATAT+AGG	0.333570	6.1:-62599977	MS.gene83292:CDS
GGACTACAAAAGTTCAATTC+TGG	0.335250	6.1:-62599912	MS.gene83292:CDS
TTACCATGTTTGTCAAATAC+TGG	0.337755	6.1:+62600344	None:intergenic
CTACAAAACAATGCTATAAC+TGG	0.349490	6.1:-62600212	MS.gene83292:CDS
GGAAAGGGTGAGGGATAAAA+CGG	0.381013	6.1:+62600418	None:intergenic
GAAACATGGATCTGGATATA+GGG	0.381905	6.1:-62599976	MS.gene83292:CDS
CTGCTAGGGTTATCACTTGC+TGG	0.383052	6.1:+62600287	None:intergenic
GATTGGTGATGAAAGCAAAA+AGG	0.386990	6.1:+62600390	None:intergenic
TATCCTGATGTAGAAATAAC+TGG	0.387236	6.1:-62600017	MS.gene83292:CDS
GAGGGATAAAACGGGTTTCA+TGG	0.388199	6.1:+62600427	None:intergenic
AAAATGTAGTATTGGCTACT+AGG	0.393835	6.1:+62600311	None:intergenic
TCTACATCAGGATAGTTTCC+AGG	0.394205	6.1:+62600026	None:intergenic
CCACCAGTTATTTCTACATC+AGG	0.397664	6.1:+62600014	None:intergenic
TTTGAAGATTCAGCACAATC+TGG	0.404925	6.1:+62600104	None:intergenic
TATTGGCTACTAGGGTTAAG+AGG	0.408482	6.1:+62600320	None:intergenic
TTAATCTTGAAACATGGATC+TGG	0.434400	6.1:-62599984	MS.gene83292:CDS
AGGGTTAGTCCTCCACTGCT+AGG	0.442500	6.1:+62600272	None:intergenic
ATTGTGCTGAATCTTCAAAA+TGG	0.450225	6.1:-62600100	MS.gene83292:CDS
CAACTAAGTTGTGTTGGTAT+TGG	0.455890	6.1:-62600053	MS.gene83292:CDS
CTAAGTTGTGTTGGTATTGG+TGG	0.456720	6.1:-62600050	MS.gene83292:CDS
CTGAATCACCGACTTTGTCT+AGG	0.458530	6.1:+62600252	None:intergenic
AGCGTTGCTTGGTGAGAGAT+TGG	0.473756	6.1:+62600373	None:intergenic
TGTGTTGGTATTGGTGGTCC+TGG	0.481842	6.1:-62600044	MS.gene83292:CDS
TTGCTGGTAAAATGTAGTAT+TGG	0.501308	6.1:+62600303	None:intergenic
AAAGGAAGAAGGAAAGGGTG+AGG	0.524873	6.1:+62600408	None:intergenic
CATGGATCTGGATATAGGGT+TGG	0.534082	6.1:-62599972	MS.gene83292:CDS
GGGTTAGTCCTCCACTGCTA+GGG	0.534912	6.1:+62600273	None:intergenic
ACTGGAGATTGTGCTTATGT+TGG	0.540839	6.1:-62599933	MS.gene83292:CDS
GAAAGGGTGAGGGATAAAAC+GGG	0.542290	6.1:+62600419	None:intergenic
ATCACTAGTAACATCGTGAC+AGG	0.548264	6.1:-62600158	MS.gene83292:CDS
ATTAATACTATGTCTAATGA+AGG	0.548963	6.1:-62599819	MS.gene83292:CDS
ATTGGCTACTAGGGTTAAGA+GGG	0.549466	6.1:+62600321	None:intergenic
TGAATCACCGACTTTGTCTA+GGG	0.549511	6.1:+62600253	None:intergenic
AAATGTAGTATTGGCTACTA+GGG	0.551896	6.1:+62600312	None:intergenic
AAGGAAGAAGGAAAGGGTGA+GGG	0.562566	6.1:+62600409	None:intergenic
CAAAAGTTCAATTCTGGTGA+AGG	0.564140	6.1:-62599906	MS.gene83292:CDS
CAACCAGTATTTGACAAACA+TGG	0.565333	6.1:-62600347	MS.gene83292:CDS
AATACTCAACTAAGTTGTGT+TGG	0.566729	6.1:-62600059	MS.gene83292:CDS
AAGTTCAATTCTGGTGAAGG+TGG	0.567292	6.1:-62599903	MS.gene83292:CDS
ACTGGTTGAACAGCGTTGCT+TGG	0.579741	6.1:+62600362	None:intergenic
AGTGAACTCGATCTCAAGAT+CGG	0.599342	6.1:+62600133	None:intergenic
AGTGATAACCCTAGCAGTGG+AGG	0.604496	6.1:-62600281	MS.gene83292:CDS
GCAAGTGATAACCCTAGCAG+TGG	0.634880	6.1:-62600284	MS.gene83292:CDS
GGACTAACCCTAGACAAAGT+CGG	0.647043	6.1:-62600260	MS.gene83292:CDS
CCTGATGTAGAAATAACTGG+TGG	0.680144	6.1:-62600014	MS.gene83292:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTTAATCTTGAAACA+TGG	-	chr6.1:62600226-62600245	MS.gene83292:CDS	15.0%
!!	ATTAATACTATGTCTAATGA+AGG	-	chr6.1:62600397-62600416	MS.gene83292:CDS	20.0%
	AATACTCAACTAAGTTGTGT+TGG	-	chr6.1:62600157-62600176	MS.gene83292:CDS	30.0%
	ATTGTGCTGAATCTTCAAAA+TGG	-	chr6.1:62600116-62600135	MS.gene83292:CDS	30.0%
	CTACAAAACAATGCTATAAC+TGG	-	chr6.1:62600004-62600023	MS.gene83292:CDS	30.0%
	TATCCTGATGTAGAAATAAC+TGG	-	chr6.1:62600199-62600218	MS.gene83292:CDS	30.0%
	TTAATCTTGAAACATGGATC+TGG	-	chr6.1:62600232-62600251	MS.gene83292:CDS	30.0%
	TTACCATGTTTGTCAAATAC+TGG	+	chr6.1:62599875-62599894	None:intergenic	30.0%
	TTGCTGGTAAAATGTAGTAT+TGG	+	chr6.1:62599916-62599935	None:intergenic	30.0%
!	ATATAGGGTTGGATTTTGTT+TGG	-	chr6.1:62600255-62600274	MS.gene83292:CDS	30.0%
!!	AAAATGTAGTATTGGCTACT+AGG	+	chr6.1:62599908-62599927	None:intergenic	30.0%
!!	AAATGTAGTATTGGCTACTA+GGG	+	chr6.1:62599907-62599926	None:intergenic	30.0%
!!	CAGTTATAGCATTGTTTTGT+AGG	+	chr6.1:62600006-62600025	None:intergenic	30.0%
	AAGCAAAAAGGAAGAAGGAA+AGG	+	chr6.1:62599817-62599836	None:intergenic	35.0%
	ACTAGTGATGTTTCCAAGTA+TGG	+	chr6.1:62600047-62600066	None:intergenic	35.0%
	AGCAAAAAGGAAGAAGGAAA+GGG	+	chr6.1:62599816-62599835	None:intergenic	35.0%
	CAAAAGTTCAATTCTGGTGA+AGG	-	chr6.1:62600310-62600329	MS.gene83292:CDS	35.0%
	CAACCAGTATTTGACAAACA+TGG	-	chr6.1:62599869-62599888	MS.gene83292:CDS	35.0%
	CCAAGTATGGTGAATTTAAC+TGG	+	chr6.1:62600034-62600053	None:intergenic	35.0%
	CCAGTTAAATTCACCATACT+TGG	-	chr6.1:62600031-62600050	MS.gene83292:CDS	35.0%
	GAAACATGGATCTGGATATA+GGG	-	chr6.1:62600240-62600259	MS.gene83292:CDS	35.0%
	GATGAAAGCAAAAAGGAAGA+AGG	+	chr6.1:62599822-62599841	None:intergenic	35.0%
	GGACTACAAAAGTTCAATTC+TGG	-	chr6.1:62600304-62600323	MS.gene83292:CDS	35.0%
	TGAAACATGGATCTGGATAT+AGG	-	chr6.1:62600239-62600258	MS.gene83292:CDS	35.0%
	TTTGAAGATTCAGCACAATC+TGG	+	chr6.1:62600115-62600134	None:intergenic	35.0%
!	CAACTAAGTTGTGTTGGTAT+TGG	-	chr6.1:62600163-62600182	MS.gene83292:CDS	35.0%
!!	GATTGGTGATGAAAGCAAAA+AGG	+	chr6.1:62599829-62599848	None:intergenic	35.0%
!!	GGATTTTGTTTGGATACAAC+TGG	-	chr6.1:62600265-62600284	MS.gene83292:CDS	35.0%
!!	TTGTTTTGTAGGACAGTTAG+TGG	+	chr6.1:62599995-62600014	None:intergenic	35.0%
	AAGTTCAATTCTGGTGAAGG+TGG	-	chr6.1:62600313-62600332	MS.gene83292:CDS	40.0%
	ACTGGAGATTGTGCTTATGT+TGG	-	chr6.1:62600283-62600302	MS.gene83292:CDS	40.0%
	AGTGAACTCGATCTCAAGAT+CGG	+	chr6.1:62600086-62600105	None:intergenic	40.0%
	ATCACTAGTAACATCGTGAC+AGG	-	chr6.1:62600058-62600077	MS.gene83292:CDS	40.0%
	ATTGGCTACTAGGGTTAAGA+GGG	+	chr6.1:62599898-62599917	None:intergenic	40.0%
	CCTGATGTAGAAATAACTGG+TGG	-	chr6.1:62600202-62600221	MS.gene83292:CDS	40.0%
	TCTACATCAGGATAGTTTCC+AGG	+	chr6.1:62600193-62600212	None:intergenic	40.0%
!	CTAAGTTGTGTTGGTATTGG+TGG	-	chr6.1:62600166-62600185	MS.gene83292:CDS	40.0%
!	TATTGGCTACTAGGGTTAAG+AGG	+	chr6.1:62599899-62599918	None:intergenic	40.0%
!!	CCACCAGTTATTTCTACATC+AGG	+	chr6.1:62600205-62600224	None:intergenic	40.0%
!!	TGAATCACCGACTTTGTCTA+GGG	+	chr6.1:62599966-62599985	None:intergenic	40.0%
	AAAGGAAGAAGGAAAGGGTG+AGG	+	chr6.1:62599811-62599830	None:intergenic	45.0%
	AAGGAAGAAGGAAAGGGTGA+GGG	+	chr6.1:62599810-62599829	None:intergenic	45.0%
	CATGGATCTGGATATAGGGT+TGG	-	chr6.1:62600244-62600263	MS.gene83292:CDS	45.0%
	GAAAGGGTGAGGGATAAAAC+GGG	+	chr6.1:62599800-62599819	None:intergenic	45.0%
	GGAAAGGGTGAGGGATAAAA+CGG	+	chr6.1:62599801-62599820	None:intergenic	45.0%
	GGACTAACCCTAGACAAAGT+CGG	-	chr6.1:62599956-62599975	MS.gene83292:CDS	45.0%
!!	CTGAATCACCGACTTTGTCT+AGG	+	chr6.1:62599967-62599986	None:intergenic	45.0%
	ACTGGTTGAACAGCGTTGCT+TGG	+	chr6.1:62599857-62599876	None:intergenic	50.0%
	AGTGATAACCCTAGCAGTGG+AGG	-	chr6.1:62599935-62599954	MS.gene83292:CDS	50.0%
	CTGCTAGGGTTATCACTTGC+TGG	+	chr6.1:62599932-62599951	None:intergenic	50.0%
	GCAAGTGATAACCCTAGCAG+TGG	-	chr6.1:62599932-62599951	MS.gene83292:CDS	50.0%
!	AGCGTTGCTTGGTGAGAGAT+TGG	+	chr6.1:62599846-62599865	None:intergenic	50.0%
!!	TGTGTTGGTATTGGTGGTCC+TGG	-	chr6.1:62600172-62600191	MS.gene83292:CDS	50.0%
!!	AGGGTTAGTCCTCCACTGCT+AGG	+	chr6.1:62599947-62599966	None:intergenic	55.0%
!!	GGGTTAGTCCTCCACTGCTA+GGG	+	chr6.1:62599946-62599965	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	62599791	62600447	62599791	ID=MS.gene83292
chr6.1	mRNA	62599791	62600447	62599791	ID=MS.gene83292.t1;Parent=MS.gene83292
chr6.1	exon	62599791	62600447	62599791	ID=MS.gene83292.t1.exon1;Parent=MS.gene83292.t1
chr6.1	CDS	62599791	62600447	62599791	ID=cds.MS.gene83292.t1;Parent=MS.gene83292.t1

Gene Sequence

>MS.gene83292

ATGAAACCCGTTTTATCCCTCACCCTTTCCTTCTTCCTTTTTGCTTTCATCACCAATCTCTCACCAAGCAACGCTGTTCAACCAGTATTTGACAAACATGGTAACCCTCTTAACCCTAGTAGCCAATACTACATTTTACCAGCAAGTGATAACCCTAGCAGTGGAGGACTAACCCTAGACAAAGTCGGTGATTCAGAGTGTCCACTAACTGTCCTACAAAACAATGCTATAACTGGTTTACCAGTTAAATTCACCATACTTGGAAACATCACTAGTAACATCGTGACAGGTACCGATCTTGAGATCGAGTTCACTAAGAAGCCAGATTGTGCTGAATCTTCAAAATGGTTGATGTTTGTTGATCATAATACTCAACTAAGTTGTGTTGGTATTGGTGGTCCTGGAAACTATCCTGATGTAGAAATAACTGGTGGCAAATTTTTAATCTTGAAACATGGATCTGGATATAGGGTTGGATTTTGTTTGGATACAACTGGAGATTGTGCTTATGTTGGACTACAAAAGTTCAATTCTGGTGAAGGTGGATCACGTTTGATTTTGACTGTAACTGATGCTTATTCTGTTGTATTTGTTGATGCTGCTTCTATTAATACTATGTCTAATGAAGGTAATGCAAATAGTGCATTGCCTATTTAG

Protein sequence

>MS.gene83292.t1

MKPVLSLTLSFFLFAFITNLSPSNAVQPVFDKHGNPLNPSSQYYILPASDNPSSGGLTLDKVGDSECPLTVLQNNAITGLPVKFTILGNITSNIVTGTDLEIEFTKKPDCAESSKWLMFVDHNTQLSCVGIGGPGNYPDVEITGGKFLILKHGSGYRVGFCLDTTGDCAYVGLQKFNSGEGGSRLILTVTDAYSVVFVDAASINTMSNEGNANSALPI