Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84537.t1 | XP_003600461.1 | 99.7 | 313 | 1 | 0 | 1 | 313 | 1 | 313 | 3.60E-186 | 660.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84537.t1 | Q9FKT5 | 85.3 | 313 | 45 | 1 | 2 | 313 | 3 | 315 | 1.5e-161 | 570.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84537.t1 | G7IY98 | 99.7 | 313 | 1 | 0 | 1 | 313 | 1 | 313 | 2.6e-186 | 660.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene84537 | MS.gene066026 | PPI |
| MS.gene84537 | MS.gene012608 | PPI |
| MS.gene84537 | MS.gene004825 | PPI |
| MS.gene84537 | MS.gene042253 | PPI |
| MS.gene84537 | MS.gene68222 | PPI |
| MS.gene84537 | MS.gene046235 | PPI |
| MS.gene056488 | MS.gene84537 | PPI |
| MS.gene84537 | MS.gene97980 | PPI |
| MS.gene84537 | MS.gene056487 | PPI |
| MS.gene84537 | MS.gene71431 | PPI |
| MS.gene84537 | MS.gene008793 | PPI |
| MS.gene95244 | MS.gene84537 | PPI |
| MS.gene97981 | MS.gene84537 | PPI |
| MS.gene84537 | MS.gene063165 | PPI |
| MS.gene064894 | MS.gene84537 | PPI |
| MS.gene84537 | MS.gene52980 | PPI |
| MS.gene33535 | MS.gene84537 | PPI |
| MS.gene84537 | MS.gene008794 | PPI |
| MS.gene84537 | MS.gene014281 | PPI |
| MS.gene84537 | MS.gene93059 | PPI |
| MS.gene84537 | MS.gene93058 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84537.t1 | MTR_3g061450 | 99.681 | 313 | 1 | 0 | 1 | 313 | 1 | 313 | 0.0 | 657 |
| MS.gene84537.t1 | MTR_4g095058 | 28.179 | 291 | 190 | 8 | 11 | 289 | 280 | 563 | 5.84e-21 | 93.6 |
| MS.gene84537.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 402 | 576 | 1.00e-14 | 75.1 |
| MS.gene84537.t1 | MTR_4g095058 | 28.179 | 291 | 190 | 8 | 11 | 289 | 278 | 561 | 6.34e-21 | 93.6 |
| MS.gene84537.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 400 | 574 | 1.03e-14 | 75.1 |
| MS.gene84537.t1 | MTR_5g016350 | 24.625 | 333 | 204 | 9 | 17 | 307 | 167 | 494 | 1.08e-16 | 80.9 |
| MS.gene84537.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 348 | 517 | 3.15e-15 | 76.3 |
| MS.gene84537.t1 | MTR_5g016350 | 25.177 | 282 | 152 | 10 | 20 | 249 | 231 | 505 | 4.36e-13 | 70.1 |
| MS.gene84537.t1 | MTR_5g016350 | 24.625 | 333 | 204 | 9 | 17 | 307 | 231 | 558 | 1.17e-16 | 80.9 |
| MS.gene84537.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 412 | 581 | 2.01e-15 | 77.0 |
| MS.gene84537.t1 | MTR_5g016350 | 25.177 | 282 | 152 | 10 | 20 | 249 | 295 | 569 | 4.63e-13 | 70.1 |
| MS.gene84537.t1 | MTR_5g016370 | 28.409 | 176 | 109 | 4 | 13 | 177 | 424 | 593 | 6.40e-16 | 78.6 |
| MS.gene84537.t1 | MTR_5g016370 | 24.625 | 333 | 202 | 11 | 17 | 307 | 245 | 570 | 6.64e-16 | 78.6 |
| MS.gene84537.t1 | MTR_5g016370 | 23.759 | 282 | 156 | 7 | 20 | 249 | 307 | 581 | 1.08e-11 | 65.9 |
| MS.gene84537.t1 | MTR_4g125710 | 24.254 | 268 | 177 | 6 | 13 | 258 | 54 | 317 | 8.88e-16 | 77.0 |
| MS.gene84537.t1 | MTR_3g106080 | 25.882 | 255 | 124 | 6 | 15 | 251 | 457 | 664 | 1.33e-15 | 77.8 |
| MS.gene84537.t1 | MTR_1g072140 | 23.051 | 295 | 213 | 8 | 11 | 297 | 19 | 307 | 4.64e-15 | 74.7 |
| MS.gene84537.t1 | MTR_1g097140 | 25.769 | 260 | 181 | 7 | 12 | 267 | 116 | 367 | 6.47e-15 | 75.1 |
| MS.gene84537.t1 | MTR_4g119620 | 24.797 | 246 | 172 | 6 | 13 | 251 | 72 | 311 | 1.30e-14 | 73.6 |
| MS.gene84537.t1 | MTR_7g086360 | 21.477 | 298 | 217 | 7 | 16 | 301 | 52 | 344 | 6.30e-14 | 71.6 |
| MS.gene84537.t1 | MTR_3g449990 | 24.653 | 288 | 203 | 9 | 17 | 295 | 268 | 550 | 1.50e-13 | 71.6 |
| MS.gene84537.t1 | MTR_5g090420 | 24.739 | 287 | 201 | 9 | 18 | 295 | 263 | 543 | 3.42e-13 | 70.5 |
| MS.gene84537.t1 | MTR_6g004040 | 27.160 | 243 | 158 | 8 | 13 | 244 | 5 | 239 | 1.36e-12 | 68.6 |
| MS.gene84537.t1 | MTR_5g022690 | 25.238 | 210 | 145 | 6 | 13 | 217 | 273 | 475 | 4.66e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84537.t1 | AT5G56130 | 85.397 | 315 | 44 | 2 | 1 | 313 | 1 | 315 | 0.0 | 569 |
| MS.gene84537.t1 | AT5G67320 | 28.328 | 293 | 187 | 11 | 11 | 289 | 315 | 598 | 2.10e-21 | 95.1 |
| MS.gene84537.t1 | AT5G67320 | 29.714 | 175 | 110 | 4 | 12 | 177 | 441 | 611 | 6.78e-15 | 75.5 |
| MS.gene84537.t1 | AT2G43770 | 23.729 | 295 | 208 | 8 | 16 | 298 | 50 | 339 | 1.32e-16 | 79.7 |
| MS.gene84537.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.18e-14 | 74.7 |
| MS.gene84537.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.18e-14 | 74.7 |
| MS.gene84537.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 1.18e-14 | 74.7 |
| MS.gene84537.t1 | AT5G25150 | 25.292 | 257 | 123 | 7 | 15 | 251 | 456 | 663 | 1.24e-14 | 74.7 |
| MS.gene84537.t1 | AT3G49660 | 25.329 | 304 | 198 | 12 | 10 | 295 | 15 | 307 | 3.44e-14 | 72.4 |
| MS.gene84537.t1 | AT3G49660 | 23.715 | 253 | 180 | 6 | 13 | 258 | 65 | 311 | 1.74e-13 | 70.5 |
| MS.gene84537.t1 | AT3G49660 | 26.442 | 208 | 140 | 6 | 17 | 217 | 111 | 312 | 4.88e-11 | 63.2 |
| MS.gene84537.t1 | AT4G02730 | 27.358 | 212 | 140 | 8 | 14 | 217 | 80 | 285 | 1.09e-13 | 71.2 |
| MS.gene84537.t1 | AT5G25150 | 27.143 | 210 | 100 | 6 | 15 | 220 | 456 | 616 | 1.82e-13 | 71.2 |
Find 79 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCATTGGTTTAAATTTC+CGG | 0.206544 | 3.1:+46121182 | None:intergenic |
| TATGCAAGTACATTGTATTT+AGG | 0.212077 | 3.1:+46116164 | None:intergenic |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.1:-46122757 | MS.gene84537:CDS |
| CTCATGTAGCTGTCGGTAAT+AGG | 0.276423 | 3.1:-46121448 | MS.gene84537:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.1:+46121762 | None:intergenic |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.1:+46121763 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.1:-46121729 | MS.gene84537:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.1:-46121752 | MS.gene84537:CDS |
| ACAATTAGTTTCAACCATAC+TGG | 0.312869 | 3.1:-46116766 | MS.gene84537:CDS |
| TGTCAGCTTGTGGGTTATTT+CGG | 0.319330 | 3.1:-46116945 | MS.gene84537:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.1:-46121751 | MS.gene84537:CDS |
| CATTACATCATATTCCTTGT+AGG | 0.364142 | 3.1:-46116214 | MS.gene84537:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.1:-46117374 | MS.gene84537:intron |
| CTGATTCCCTTGTCAGCTTG+TGG | 0.395348 | 3.1:-46116955 | MS.gene84537:CDS |
| TGATTCCCTTGTCAGCTTGT+GGG | 0.409663 | 3.1:-46116954 | MS.gene84537:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.1:-46122997 | MS.gene84537:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.1:-46117155 | MS.gene84537:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.1:-46122814 | MS.gene84537:intron |
| CAATTAGTTTCAACCATACT+GGG | 0.443807 | 3.1:-46116765 | MS.gene84537:CDS |
| CCTAGGTAAATGAGATTGCT+TGG | 0.445389 | 3.1:-46117415 | MS.gene84537:intron |
| ATGAGTCCCATCAGGTTTGT+AGG | 0.446382 | 3.1:+46121466 | None:intergenic |
| GGTCAAAGATATTGAATTGA+AGG | 0.446383 | 3.1:-46121821 | MS.gene84537:CDS |
| ATTCCACTCAACACTATTCA+TGG | 0.456424 | 3.1:+46116187 | None:intergenic |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.1:-46117380 | MS.gene84537:CDS |
| ATTGGCACAAAACTTGCTTC+TGG | 0.457664 | 3.1:-46122785 | MS.gene84537:CDS |
| TCATGTAGCTGTCGGTAATA+GGG | 0.457984 | 3.1:-46121447 | MS.gene84537:intron |
| TGAGACAGACATTTCGCTGT+TGG | 0.470477 | 3.1:-46116983 | MS.gene84537:intron |
| TACAATGTACTTGCATATGC+TGG | 0.472545 | 3.1:-46116158 | MS.gene84537:CDS |
| AAGCAATCAAGTCCCCAGTA+TGG | 0.482571 | 3.1:+46116752 | None:intergenic |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.488476 | 3.1:-46116789 | MS.gene84537:intron |
| ATTACATCATATTCCTTGTA+GGG | 0.491750 | 3.1:-46116213 | MS.gene84537:CDS |
| AGGTGGAAAATGCTCACAAC+AGG | 0.493748 | 3.1:-46121519 | MS.gene84537:CDS |
| TATGGTTGAAACTAATTGTC+CGG | 0.499723 | 3.1:+46116770 | None:intergenic |
| CTATTCATGGCAGCCCTACA+AGG | 0.501447 | 3.1:+46116200 | None:intergenic |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.1:+46121753 | None:intergenic |
| AGAAGCATTACCTTCCTGTT+GGG | 0.503474 | 3.1:+46117141 | None:intergenic |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.1:-46121505 | MS.gene84537:CDS |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.1:+46117206 | None:intergenic |
| TTGAACTTGCGCCTATGCAT+TGG | 0.510346 | 3.1:+46121169 | None:intergenic |
| GTGACAGTCCAATGTTCATA+CGG | 0.510916 | 3.1:-46116243 | MS.gene84537:intron |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.1:-46121728 | MS.gene84537:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.1:+46116904 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.1:-46122803 | MS.gene84537:CDS |
| AGTTAACAATACTTGATGTC+CGG | 0.522172 | 3.1:-46121201 | MS.gene84537:CDS |
| AAATGAGAATTGTTCCTCCA+TGG | 0.522481 | 3.1:+46123026 | None:intergenic |
| AAGAAGCATTACCTTCCTGT+TGG | 0.526316 | 3.1:+46117140 | None:intergenic |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.1:-46121506 | MS.gene84537:CDS |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.1:+46121754 | None:intergenic |
| TGACAGTCCAATGTTCATAC+GGG | 0.537123 | 3.1:-46116242 | MS.gene84537:intron |
| AACATCACCTACAAACCTGA+TGG | 0.546702 | 3.1:-46121473 | MS.gene84537:CDS |
| AAGGTTACTACATGGGCATG+TGG | 0.551642 | 3.1:+46122725 | None:intergenic |
| GTCAAAGATATTGAATTGAA+GGG | 0.552936 | 3.1:-46121820 | MS.gene84537:CDS |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.1:-46117186 | MS.gene84537:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.1:-46117185 | MS.gene84537:CDS |
| TAGTGTGGATCAGCTATGTT+GGG | 0.558977 | 3.1:-46121788 | MS.gene84537:CDS |
| AAATAACCCACAAGCTGACA+AGG | 0.562424 | 3.1:+46116948 | None:intergenic |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.1:-46117198 | MS.gene84537:CDS |
| ACAGCTACATGAGTCCCATC+AGG | 0.569784 | 3.1:+46121458 | None:intergenic |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.1:+46116775 | None:intergenic |
| TAGGCGCAAGTTCAACTATG+AGG | 0.599831 | 3.1:-46121161 | MS.gene84537:intron |
| TTTCGGAAATGCTTTGTGTG+CGG | 0.599990 | 3.1:-46116928 | MS.gene84537:CDS |
| ATAGTGTGGATCAGCTATGT+TGG | 0.602960 | 3.1:-46121789 | MS.gene84537:CDS |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.1:-46117404 | MS.gene84537:CDS |
| GGAAATTTAAACCAATGCAT+AGG | 0.603824 | 3.1:-46121180 | MS.gene84537:CDS |
| GAAGGGTCACACTGATAGTG+TGG | 0.604053 | 3.1:-46121803 | MS.gene84537:CDS |
| AGGTTACTACATGGGCATGT+GGG | 0.610892 | 3.1:+46122726 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.1:+46121764 | None:intergenic |
| GATGGGACTCATGTAGCTGT+CGG | 0.632901 | 3.1:-46121455 | MS.gene84537:CDS |
| AACAAGTATCAGGCTGACGA+AGG | 0.633197 | 3.1:-46116125 | MS.gene84537:intron |
| ACATCACCTACAAACCTGAT+GGG | 0.634892 | 3.1:-46121472 | MS.gene84537:CDS |
| GTTTGAAAAGGTGCATTCAG+TGG | 0.648774 | 3.1:-46122819 | MS.gene84537:intron |
| CTGCCATGAATAGTGTTGAG+TGG | 0.656405 | 3.1:-46116190 | MS.gene84537:CDS |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.1:-46117151 | MS.gene84537:intron |
| GCTCACGTACTCAGGGACTG+TGG | 0.668767 | 3.1:-46117243 | MS.gene84537:intron |
| AATAACCCACAAGCTGACAA+GGG | 0.681570 | 3.1:+46116949 | None:intergenic |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.1:+46117194 | None:intergenic |
| GACAGTCCAATGTTCATACG+GGG | 0.704919 | 3.1:-46116241 | MS.gene84537:intron |
| AATTAGTTTCAACCATACTG+GGG | 0.712478 | 3.1:-46116764 | MS.gene84537:CDS |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.1:-46121504 | MS.gene84537:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 46116004 | 46123046 | 46116004 | ID=MS.gene84537 |
| chr3.1 | mRNA | 46116004 | 46123046 | 46116004 | ID=MS.gene84537.t1;Parent=MS.gene84537 |
| chr3.1 | exon | 46122984 | 46123046 | 46122984 | ID=MS.gene84537.t1.exon1;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46122984 | 46123046 | 46122984 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46122736 | 46122831 | 46122736 | ID=MS.gene84537.t1.exon2;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46122736 | 46122831 | 46122736 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46121719 | 46121848 | 46121719 | ID=MS.gene84537.t1.exon3;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46121719 | 46121848 | 46121719 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46121448 | 46121539 | 46121448 | ID=MS.gene84537.t1.exon4;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46121448 | 46121539 | 46121448 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46121162 | 46121230 | 46121162 | ID=MS.gene84537.t1.exon5;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46121162 | 46121230 | 46121162 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46117375 | 46117432 | 46117375 | ID=MS.gene84537.t1.exon6;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46117375 | 46117432 | 46117375 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46117152 | 46117251 | 46117152 | ID=MS.gene84537.t1.exon7;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46117152 | 46117251 | 46117152 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46116911 | 46116997 | 46116911 | ID=MS.gene84537.t1.exon8;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46116911 | 46116997 | 46116911 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46116723 | 46116801 | 46116723 | ID=MS.gene84537.t1.exon9;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46116723 | 46116801 | 46116723 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46116126 | 46116258 | 46116126 | ID=MS.gene84537.t1.exon10;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46116126 | 46116258 | 46116126 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| chr3.1 | exon | 46116004 | 46116038 | 46116004 | ID=MS.gene84537.t1.exon11;Parent=MS.gene84537.t1 |
| chr3.1 | CDS | 46116004 | 46116038 | 46116004 | ID=cds.MS.gene84537.t1;Parent=MS.gene84537.t1 |
| Gene Sequence |
| Protein sequence |
