Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86434.t1 | XP_003597940.1 | 98.1 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 3.50E-100 | 374.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86434.t1 | G7IIA4 | 98.1 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 2.5e-100 | 374.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050226 | MS.gene86434 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene86434 | 0.816698 | 4.84E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene86434 | 0.816325 | 5.86E-52 | -1.69E-46 |
| MS.gene050330 | MS.gene86434 | -0.810137 | 1.34E-50 | -1.69E-46 |
| MS.gene050680 | MS.gene86434 | 0.801969 | 7.00E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene86434 | 0.818092 | 2.35E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene86434 | 0.808246 | 3.40E-50 | -1.69E-46 |
| MS.gene051265 | MS.gene86434 | 0.810528 | 1.10E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene86434 | 0.800494 | 1.40E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene86434 | 0.814266 | 1.68E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene86434 | 0.814276 | 1.67E-51 | -1.69E-46 |
| MS.gene052389 | MS.gene86434 | 0.805227 | 1.48E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene86434 | -0.803685 | 3.10E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene86434 | 0.818531 | 1.87E-52 | -1.69E-46 |
| MS.gene053201 | MS.gene86434 | 0.805052 | 1.61E-49 | -1.69E-46 |
| MS.gene054093 | MS.gene86434 | 0.801014 | 1.10E-48 | -1.69E-46 |
| MS.gene054468 | MS.gene86434 | 0.814928 | 1.20E-51 | -1.69E-46 |
| MS.gene054559 | MS.gene86434 | 0.823957 | 1.05E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene86434 | 0.804005 | 2.66E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene86434 | 0.838119 | 3.47E-57 | -1.69E-46 |
| MS.gene055357 | MS.gene86434 | 0.800831 | 1.20E-48 | -1.69E-46 |
| MS.gene055358 | MS.gene86434 | 0.843604 | 1.27E-58 | -1.69E-46 |
| MS.gene055367 | MS.gene86434 | 0.809665 | 1.69E-50 | -1.69E-46 |
| MS.gene057200 | MS.gene86434 | 0.804689 | 1.92E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene86434 | 0.803785 | 2.95E-49 | -1.69E-46 |
| MS.gene058426 | MS.gene86434 | 0.806129 | 9.56E-50 | -1.69E-46 |
| MS.gene058736 | MS.gene86434 | 0.80794 | 3.95E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene86434 | 0.835049 | 2.10E-56 | -1.69E-46 |
| MS.gene059247 | MS.gene86434 | 0.802311 | 5.96E-49 | -1.69E-46 |
| MS.gene059306 | MS.gene86434 | 0.816609 | 5.06E-52 | -1.69E-46 |
| MS.gene061114 | MS.gene86434 | -0.858112 | 1.03E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86434.t1 | MTR_2g104230 | 98.095 | 210 | 3 | 1 | 1 | 210 | 1 | 209 | 5.65e-155 | 427 |
| MS.gene86434.t1 | MTR_4g009760 | 59.729 | 221 | 65 | 10 | 1 | 210 | 1 | 208 | 9.74e-81 | 239 |
| MS.gene86434.t1 | MTR_4g116200 | 45.912 | 159 | 70 | 3 | 1 | 158 | 1 | 144 | 4.52e-34 | 122 |
| MS.gene86434.t1 | MTR_2g450720 | 38.378 | 185 | 86 | 6 | 1 | 165 | 1 | 177 | 1.72e-29 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86434.t1 | AT1G15760 | 44.000 | 225 | 80 | 8 | 3 | 209 | 5 | 201 | 4.46e-57 | 179 |
| MS.gene86434.t1 | AT1G80520 | 47.032 | 219 | 78 | 8 | 1 | 209 | 1 | 191 | 3.95e-50 | 161 |
| MS.gene86434.t1 | AT2G12462 | 28.846 | 260 | 122 | 7 | 1 | 209 | 1 | 248 | 1.79e-25 | 99.8 |
Find 43 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAGGCCTAATGGTGGATT+TGG | 0.285518 | 2.1:-1638035 | MS.gene86434:CDS |
| GCTTTGTCTGGTGATCTTGA+TGG | 0.316091 | 2.1:-1637897 | MS.gene86434:CDS |
| GAGAATTTCTAATCTGTGTT+TGG | 0.325351 | 2.1:+1638199 | None:intergenic |
| TAAGTGGGATACCATGTTTA+AGG | 0.337355 | 2.1:-1637754 | MS.gene86434:CDS |
| GAGAAAATGTTTCTCACAAA+TGG | 0.339341 | 2.1:-1637954 | MS.gene86434:CDS |
| CCATCTCTTGTATATGAGTA+TGG | 0.354378 | 2.1:-1638308 | MS.gene86434:CDS |
| TACTGAAGAATCAGGACTTA+TGG | 0.367073 | 2.1:-1638066 | MS.gene86434:CDS |
| AGTACTTAGCTAACTATGTT+AGG | 0.383843 | 2.1:-1638100 | MS.gene86434:CDS |
| TTCTAATCTGTGTTTGGCTA+TGG | 0.404684 | 2.1:+1638205 | None:intergenic |
| ATAATCATGATGATGGTTAT+TGG | 0.406535 | 2.1:-1637797 | MS.gene86434:CDS |
| CTAGGCCTAATGGTGGATTT+GGG | 0.437057 | 2.1:-1638034 | MS.gene86434:CDS |
| TTGACTCATACTGAAGAATC+AGG | 0.442832 | 2.1:-1638074 | MS.gene86434:CDS |
| ACAGTAGCTCCTGCTTTGTC+TGG | 0.454059 | 2.1:-1637909 | MS.gene86434:CDS |
| GAAGAGGAACACACAATTTG+TGG | 0.477404 | 2.1:-1637988 | MS.gene86434:CDS |
| CCATGCTTTGTAGAAACTCA+TGG | 0.497469 | 2.1:+1638234 | None:intergenic |
| TTAACTCAACAGCCATGGAT+TGG | 0.500177 | 2.1:-1638361 | None:intergenic |
| TTTCCAAAAGGAGCAAAAGA+TGG | 0.506781 | 2.1:-1637850 | MS.gene86434:CDS |
| AATCCACCATTAGGCCTAGT+TGG | 0.510240 | 2.1:+1638038 | None:intergenic |
| ATGGTGGATTTGGGAGAAGA+TGG | 0.518326 | 2.1:-1638025 | MS.gene86434:CDS |
| CCATGAGTTTCTACAAAGCA+TGG | 0.527981 | 2.1:-1638234 | MS.gene86434:CDS |
| CAAAAGATGGATGGAGATGA+TGG | 0.529408 | 2.1:-1637837 | MS.gene86434:CDS |
| TACAAGAGATGGCTCAAGGT+TGG | 0.536104 | 2.1:+1638319 | None:intergenic |
| GACTTATGGTGATGCCAACT+AGG | 0.538549 | 2.1:-1638052 | MS.gene86434:CDS |
| CTGCTGTTGAAGAGATTAAG+TGG | 0.538740 | 2.1:-1637770 | MS.gene86434:CDS |
| CTTCTCCCAAATCCACCATT+AGG | 0.539738 | 2.1:+1638029 | None:intergenic |
| GTGATGCCAACTAGGCCTAA+TGG | 0.547391 | 2.1:-1638044 | MS.gene86434:CDS |
| GATGGAGATGATGGTGGTGA+TGG | 0.550508 | 2.1:-1637828 | MS.gene86434:CDS |
| TGTTGTGTATCATTTCCAAA+AGG | 0.557274 | 2.1:-1637862 | MS.gene86434:CDS |
| CAAAAGGAGCAAAAGATGGA+TGG | 0.561894 | 2.1:-1637846 | MS.gene86434:CDS |
| AATAATGATAATCATGATGA+TGG | 0.562055 | 2.1:-1637804 | MS.gene86434:CDS |
| CCATACTCATATACAAGAGA+TGG | 0.573633 | 2.1:+1638308 | None:intergenic |
| TCAAGATCACCAGACAAAGC+AGG | 0.592387 | 2.1:+1637900 | None:intergenic |
| CATGAGTTTCTACAAAGCAT+GGG | 0.601787 | 2.1:-1638233 | MS.gene86434:CDS |
| GGAAGAGTGCTATAATGAAG+AGG | 0.605141 | 2.1:-1638004 | MS.gene86434:CDS |
| AAATGTTTCTCACAAATGGA+AGG | 0.609404 | 2.1:-1637950 | MS.gene86434:CDS |
| CATATACAAGAGATGGCTCA+AGG | 0.614307 | 2.1:+1638315 | None:intergenic |
| AATTTGTAGCTGCAATCAAG+AGG | 0.614506 | 2.1:-1638130 | MS.gene86434:CDS |
| TAGCCATGAAAACCAATCCA+TGG | 0.623251 | 2.1:+1638349 | None:intergenic |
| TGCTGTTGAAGAGATTAAGT+GGG | 0.633418 | 2.1:-1637769 | MS.gene86434:CDS |
| TGGCTTTAGATCCTTAAACA+TGG | 0.634954 | 2.1:+1637743 | None:intergenic |
| ATGCCAACTAGGCCTAATGG+TGG | 0.656564 | 2.1:-1638041 | MS.gene86434:CDS |
| GAGGAACACACAATTTGTGG+TGG | 0.682058 | 2.1:-1637985 | MS.gene86434:CDS |
| AAGATGGATGGAGATGATGG+TGG | 0.707845 | 2.1:-1637834 | MS.gene86434:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATGATAATCATGATGA+TGG | - | chr2.1:1638280-1638299 | MS.gene86434:CDS | 20.0% |
| !!! | ATAATCATGATGATGGTTAT+TGG | - | chr2.1:1638287-1638306 | MS.gene86434:CDS | 25.0% |
| AAATGTTTCTCACAAATGGA+AGG | - | chr2.1:1638134-1638153 | MS.gene86434:CDS | 30.0% | |
| AGTACTTAGCTAACTATGTT+AGG | - | chr2.1:1637984-1638003 | MS.gene86434:CDS | 30.0% | |
| GAGAAAATGTTTCTCACAAA+TGG | - | chr2.1:1638130-1638149 | MS.gene86434:CDS | 30.0% | |
| GAGAATTTCTAATCTGTGTT+TGG | + | chr2.1:1637888-1637907 | None:intergenic | 30.0% | |
| TGTTGTGTATCATTTCCAAA+AGG | - | chr2.1:1638222-1638241 | MS.gene86434:CDS | 30.0% | |
| !! | TTTTGGAAATGATACACAAC+AGG | + | chr2.1:1638223-1638242 | None:intergenic | 30.0% |
| AATTTGTAGCTGCAATCAAG+AGG | - | chr2.1:1637954-1637973 | MS.gene86434:CDS | 35.0% | |
| CATGAGTTTCTACAAAGCAT+GGG | - | chr2.1:1637851-1637870 | MS.gene86434:CDS | 35.0% | |
| CCATACTCATATACAAGAGA+TGG | + | chr2.1:1637779-1637798 | None:intergenic | 35.0% | |
| CCATCTCTTGTATATGAGTA+TGG | - | chr2.1:1637776-1637795 | MS.gene86434:CDS | 35.0% | |
| TAAGTGGGATACCATGTTTA+AGG | - | chr2.1:1638330-1638349 | MS.gene86434:CDS | 35.0% | |
| TACTGAAGAATCAGGACTTA+TGG | - | chr2.1:1638018-1638037 | MS.gene86434:CDS | 35.0% | |
| TGCTGTTGAAGAGATTAAGT+GGG | - | chr2.1:1638315-1638334 | MS.gene86434:CDS | 35.0% | |
| TTCTAATCTGTGTTTGGCTA+TGG | + | chr2.1:1637882-1637901 | None:intergenic | 35.0% | |
| TTGACTCATACTGAAGAATC+AGG | - | chr2.1:1638010-1638029 | MS.gene86434:CDS | 35.0% | |
| TTTCCAAAAGGAGCAAAAGA+TGG | - | chr2.1:1638234-1638253 | MS.gene86434:CDS | 35.0% | |
| ! | ACAAATTTTGCAACTGTGTG+TGG | + | chr2.1:1637941-1637960 | None:intergenic | 35.0% |
| !! | TGGCTTTAGATCCTTAAACA+TGG | + | chr2.1:1638344-1638363 | None:intergenic | 35.0% |
| CAAAAGATGGATGGAGATGA+TGG | - | chr2.1:1638247-1638266 | MS.gene86434:CDS | 40.0% | |
| CAAAAGGAGCAAAAGATGGA+TGG | - | chr2.1:1638238-1638257 | MS.gene86434:CDS | 40.0% | |
| CATATACAAGAGATGGCTCA+AGG | + | chr2.1:1637772-1637791 | None:intergenic | 40.0% | |
| CCATGAGTTTCTACAAAGCA+TGG | - | chr2.1:1637850-1637869 | MS.gene86434:CDS | 40.0% | |
| CCATGCTTTGTAGAAACTCA+TGG | + | chr2.1:1637853-1637872 | None:intergenic | 40.0% | |
| CTGCTGTTGAAGAGATTAAG+TGG | - | chr2.1:1638314-1638333 | MS.gene86434:CDS | 40.0% | |
| GAAGAGGAACACACAATTTG+TGG | - | chr2.1:1638096-1638115 | MS.gene86434:CDS | 40.0% | |
| ! | AAAGCAGGAGCTACTGTTTT+TGG | + | chr2.1:1638172-1638191 | None:intergenic | 40.0% |
| ! | CATCCATCTTTTGCTCCTTT+TGG | + | chr2.1:1638240-1638259 | None:intergenic | 40.0% |
| ! | GCTCATTTTGTGCAAAGACA+AGG | + | chr2.1:1637802-1637821 | None:intergenic | 40.0% |
| !! | GGAAGAGTGCTATAATGAAG+AGG | - | chr2.1:1638080-1638099 | MS.gene86434:CDS | 40.0% |
| AATCCACCATTAGGCCTAGT+TGG | + | chr2.1:1638049-1638068 | None:intergenic | 45.0% | |
| CTTCTCCCAAATCCACCATT+AGG | + | chr2.1:1638058-1638077 | None:intergenic | 45.0% | |
| GAGGAACACACAATTTGTGG+TGG | - | chr2.1:1638099-1638118 | MS.gene86434:CDS | 45.0% | |
| GCTTTGTCTGGTGATCTTGA+TGG | - | chr2.1:1638187-1638206 | MS.gene86434:CDS | 45.0% | |
| TACAAGAGATGGCTCAAGGT+TGG | + | chr2.1:1637768-1637787 | None:intergenic | 45.0% | |
| TCAAGATCACCAGACAAAGC+AGG | + | chr2.1:1638187-1638206 | None:intergenic | 45.0% | |
| ! | AAGATGGATGGAGATGATGG+TGG | - | chr2.1:1638250-1638269 | MS.gene86434:CDS | 45.0% |
| ! | GACTTATGGTGATGCCAACT+AGG | - | chr2.1:1638032-1638051 | MS.gene86434:CDS | 45.0% |
| !! | ACTAGGCCTAATGGTGGATT+TGG | - | chr2.1:1638049-1638068 | MS.gene86434:CDS | 45.0% |
| !! | ATGGTGGATTTGGGAGAAGA+TGG | - | chr2.1:1638059-1638078 | MS.gene86434:CDS | 45.0% |
| !! | CTAGGCCTAATGGTGGATTT+GGG | - | chr2.1:1638050-1638069 | MS.gene86434:CDS | 45.0% |
| !!! | AGGAGCTACTGTTTTTGGCA+TGG | + | chr2.1:1638167-1638186 | None:intergenic | 45.0% |
| ACAGTAGCTCCTGCTTTGTC+TGG | - | chr2.1:1638175-1638194 | MS.gene86434:CDS | 50.0% | |
| ATGCCAACTAGGCCTAATGG+TGG | - | chr2.1:1638043-1638062 | MS.gene86434:CDS | 50.0% | |
| GTGATGCCAACTAGGCCTAA+TGG | - | chr2.1:1638040-1638059 | MS.gene86434:CDS | 50.0% | |
| !! | GATGGAGATGATGGTGGTGA+TGG | - | chr2.1:1638256-1638275 | MS.gene86434:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 1637737 | 1638369 | 1637737 | ID=MS.gene86434 |
| chr2.1 | mRNA | 1637737 | 1638369 | 1637737 | ID=MS.gene86434.t1;Parent=MS.gene86434 |
| chr2.1 | exon | 1637737 | 1638369 | 1637737 | ID=MS.gene86434.t1.exon1;Parent=MS.gene86434.t1 |
| chr2.1 | CDS | 1637737 | 1638369 | 1637737 | ID=cds.MS.gene86434.t1;Parent=MS.gene86434.t1 |
| Gene Sequence |
| Protein sequence |
