Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88733.t1 | XP_003615573.1 | 100 | 161 | 0 | 0 | 1 | 161 | 1 | 161 | 5.50E-90 | 340.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88733.t1 | G7KE40 | 100.0 | 161 | 0 | 0 | 1 | 161 | 1 | 161 | 4.0e-90 | 340.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene88733 | 0.837468 | 5.11E-57 | -1.69E-46 |
| MS.gene050320 | MS.gene88733 | 0.825391 | 4.81E-54 | -1.69E-46 |
| MS.gene051063 | MS.gene88733 | 0.801611 | 8.30E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene88733 | 0.847856 | 8.87E-60 | -1.69E-46 |
| MS.gene051330 | MS.gene88733 | 0.823386 | 1.42E-53 | -1.69E-46 |
| MS.gene051746 | MS.gene88733 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene88733 | 0.808398 | 3.16E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene88733 | 0.846088 | 2.70E-59 | -1.69E-46 |
| MS.gene053109 | MS.gene88733 | 0.809561 | 1.78E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene88733 | 0.822783 | 1.97E-53 | -1.69E-46 |
| MS.gene057079 | MS.gene88733 | 0.816233 | 6.14E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene88733 | 0.828489 | 8.74E-55 | -1.69E-46 |
| MS.gene057234 | MS.gene88733 | 0.840181 | 1.01E-57 | -1.69E-46 |
| MS.gene057452 | MS.gene88733 | -0.812961 | 3.26E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene88733 | 0.821857 | 3.23E-53 | -1.69E-46 |
| MS.gene058055 | MS.gene88733 | 0.8 | 1.77E-48 | -1.69E-46 |
| MS.gene058238 | MS.gene88733 | 0.859756 | 3.32E-63 | -1.69E-46 |
| MS.gene058906 | MS.gene88733 | 0.804769 | 1.84E-49 | -1.69E-46 |
| MS.gene06001 | MS.gene88733 | 0.842248 | 2.90E-58 | -1.69E-46 |
| MS.gene060297 | MS.gene88733 | 0.80922 | 2.11E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88733.t1 | MTR_5g069660 | 100.000 | 161 | 0 | 0 | 1 | 161 | 1 | 161 | 1.60e-118 | 331 |
| MS.gene88733.t1 | MTR_3g462700 | 46.012 | 163 | 70 | 5 | 4 | 160 | 4 | 154 | 2.92e-41 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88733.t1 | AT5G58920 | 63.636 | 165 | 52 | 2 | 1 | 160 | 1 | 162 | 2.24e-74 | 220 |
Find 47 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGGAGGAGTTTATTAAAA+AGG | 0.222603 | 5.4:-54462193 | MS.gene88733:CDS |
| AGAAAGGTACGTACCTGTTT+TGG | 0.239593 | 5.4:+54462550 | None:intergenic |
| GGCCGAATAACTACTACTTT+TGG | 0.301765 | 5.4:-54462389 | MS.gene88733:CDS |
| TGAAGTTATTGGTGAGCTTC+AGG | 0.305838 | 5.4:-54462037 | MS.gene88733:CDS |
| TCATTCTCCACCATCCCTTT+AGG | 0.312626 | 5.4:+54462144 | None:intergenic |
| TTCCAAAAGTAGTAGTTATT+CGG | 0.328166 | 5.4:+54462387 | None:intergenic |
| AGGGATGGTGGAGAATGATA+TGG | 0.349759 | 5.4:-54462139 | MS.gene88733:CDS |
| GAAACAAATGGGGAAGGCTT+TGG | 0.362030 | 5.4:-54462367 | MS.gene88733:CDS |
| TGATTATAAGAAGGAGCTTA+AGG | 0.365177 | 5.4:-54462109 | MS.gene88733:CDS |
| TCCAGCTCTTTCTCTTCCTT+CGG | 0.366817 | 5.4:+54462264 | None:intergenic |
| AGCAAGAGGCTCAGAAGCTT+TGG | 0.366979 | 5.4:-54462074 | MS.gene88733:CDS |
| AGGCTCAGAAGCTTTGGTTT+AGG | 0.390308 | 5.4:-54462068 | MS.gene88733:CDS |
| TTTCTCAAGAAAATAGGGTT+GGG | 0.395517 | 5.4:-54462411 | MS.gene88733:CDS |
| GGGATGATTGGAACAACTAT+TGG | 0.398836 | 5.4:-54462168 | MS.gene88733:CDS |
| GCGGCGTGAGTTTCCGTATC+CGG | 0.401331 | 5.4:-54462229 | MS.gene88733:CDS |
| TCTTTCTCTTCCTTCGGCAT+TGG | 0.403880 | 5.4:+54462270 | None:intergenic |
| AGGATGAGGAATGAAGTTAT+TGG | 0.417045 | 5.4:-54462048 | MS.gene88733:CDS |
| GCTTCCTCGTCTCCCGGATA+CGG | 0.434151 | 5.4:+54462216 | None:intergenic |
| GAAACACCGCAACCCAAAAC+AGG | 0.459747 | 5.4:-54462563 | MS.gene88733:intron |
| TCCGGGAGACGAGGAAGCTA+TGG | 0.461416 | 5.4:-54462211 | MS.gene88733:CDS |
| AGAAGCTTTGGTTTAGGATG+AGG | 0.464134 | 5.4:-54462062 | MS.gene88733:CDS |
| GGAACAACTATTGGACCTAA+AGG | 0.464559 | 5.4:-54462159 | MS.gene88733:CDS |
| TCCATAGCTTCCTCGTCTCC+CGG | 0.480393 | 5.4:+54462210 | None:intergenic |
| GAACAACTATTGGACCTAAA+GGG | 0.484376 | 5.4:-54462158 | MS.gene88733:CDS |
| GGATAAGAAGTTTGAGCAAG+AGG | 0.490071 | 5.4:-54462088 | MS.gene88733:CDS |
| TATTGGTGAGCTTCAGGAGA+AGG | 0.502673 | 5.4:-54462031 | MS.gene88733:CDS |
| TTCTCAAGAAAATAGGGTTG+GGG | 0.508401 | 5.4:-54462410 | MS.gene88733:CDS |
| ATTCAACGAGACATCACCAT+TGG | 0.517134 | 5.4:-54462310 | MS.gene88733:CDS |
| CGGCGTGAGTTTCCGTATCC+GGG | 0.520100 | 5.4:-54462228 | MS.gene88733:CDS |
| AGACATCACCATTGGGTTGG+TGG | 0.520709 | 5.4:-54462302 | MS.gene88733:CDS |
| GGATGAGAGTGATTATAAGA+AGG | 0.532632 | 5.4:-54462118 | MS.gene88733:CDS |
| GCCGAAGGAAGAGAAAGAGC+TGG | 0.546418 | 5.4:-54462265 | MS.gene88733:CDS |
| ATTGGTGAGCTTCAGGAGAA+GGG | 0.553628 | 5.4:-54462030 | MS.gene88733:CDS |
| GGCATGCACATATGCAGTGA+TGG | 0.557186 | 5.4:-54462346 | MS.gene88733:CDS |
| TTCAACGAGACATCACCATT+GGG | 0.572234 | 5.4:-54462309 | MS.gene88733:CDS |
| ACGAGACATCACCATTGGGT+TGG | 0.576608 | 5.4:-54462305 | MS.gene88733:CDS |
| AGCTGGAACATTTGTATCAG+CGG | 0.579371 | 5.4:-54462248 | MS.gene88733:CDS |
| GACGTTGAAGCCAATGCCGA+AGG | 0.592265 | 5.4:-54462280 | MS.gene88733:CDS |
| GTTTCGAAGAAGCGTTTACG+AGG | 0.608927 | 5.4:+54462581 | None:intergenic |
| GGGAGACGAGGAAGCTATGG+AGG | 0.612133 | 5.4:-54462208 | MS.gene88733:CDS |
| AACTATTGGACCTAAAGGGA+TGG | 0.616902 | 5.4:-54462154 | MS.gene88733:CDS |
| CACATATGCAGTGATGGGTG+CGG | 0.624041 | 5.4:-54462340 | MS.gene88733:CDS |
| TATTGGACCTAAAGGGATGG+TGG | 0.625420 | 5.4:-54462151 | MS.gene88733:CDS |
| GCATGCACATATGCAGTGAT+GGG | 0.628475 | 5.4:-54462345 | MS.gene88733:CDS |
| TTCAACGTCCACCAACCCAA+TGG | 0.635572 | 5.4:+54462294 | None:intergenic |
| GTTTCCGTATCCGGGAGACG+AGG | 0.643395 | 5.4:-54462220 | MS.gene88733:CDS |
| ATGCAGTGATGGGTGCGGCG+TGG | 0.715337 | 5.4:-54462335 | MS.gene88733:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATAAAATGTAAAAAATTAT+TGG | + | chr5.4:54462140-54462159 | None:intergenic | 10.0% |
| !!! | AATATTTTCTCAAGAAAATA+GGG | - | chr5.4:54462179-54462198 | MS.gene88733:CDS | 15.0% |
| !!! | TATTTTCTTGAGAAAATATT+GGG | + | chr5.4:54462179-54462198 | None:intergenic | 15.0% |
| !!! | AATTTTTTACATTTTATGCC+AGG | - | chr5.4:54462142-54462161 | MS.gene88733:CDS | 20.0% |
| !!! | CAATATTTTCTCAAGAAAAT+AGG | - | chr5.4:54462178-54462197 | MS.gene88733:CDS | 20.0% |
| !!! | CTATTTTCTTGAGAAAATAT+TGG | + | chr5.4:54462180-54462199 | None:intergenic | 20.0% |
| ! | ATGGAGGAGTTTATTAAAAA+GGG | - | chr5.4:54462403-54462422 | MS.gene88733:CDS | 25.0% |
| ! | TATGGAGGAGTTTATTAAAA+AGG | - | chr5.4:54462402-54462421 | MS.gene88733:CDS | 25.0% |
| ! | TTCCAAAAGTAGTAGTTATT+CGG | + | chr5.4:54462211-54462230 | None:intergenic | 25.0% |
| !! | GTGTAATAGTAAGTGTTTTA+AGG | + | chr5.4:54462076-54462095 | None:intergenic | 25.0% |
| !!! | TAATAGTAAGTGTTTTAAGG+TGG | + | chr5.4:54462073-54462092 | None:intergenic | 25.0% |
| !!! | TACTACTTTTGGAAACAAAT+GGG | - | chr5.4:54462217-54462236 | MS.gene88733:CDS | 25.0% |
| TGATTATAAGAAGGAGCTTA+AGG | - | chr5.4:54462486-54462505 | MS.gene88733:intron | 30.0% | |
| TGGAGGAGTTTATTAAAAAG+GGG | - | chr5.4:54462404-54462423 | MS.gene88733:CDS | 30.0% | |
| TTTCTCAAGAAAATAGGGTT+GGG | - | chr5.4:54462184-54462203 | MS.gene88733:CDS | 30.0% | |
| ! | TTTTCTCAAGAAAATAGGGT+TGG | - | chr5.4:54462183-54462202 | MS.gene88733:CDS | 30.0% |
| !! | ACTACTTTTGGAAACAAATG+GGG | - | chr5.4:54462218-54462237 | MS.gene88733:CDS | 30.0% |
| !! | CTACTACTTTTGGAAACAAA+TGG | - | chr5.4:54462216-54462235 | MS.gene88733:CDS | 30.0% |
| AGGAGTTTATTAAAAAGGGG+GGG | - | chr5.4:54462407-54462426 | MS.gene88733:CDS | 35.0% | |
| AGGATGAGGAATGAAGTTAT+TGG | - | chr5.4:54462547-54462566 | MS.gene88733:intron | 35.0% | |
| GAACAACTATTGGACCTAAA+GGG | - | chr5.4:54462437-54462456 | MS.gene88733:intron | 35.0% | |
| GAGGAGTTTATTAAAAAGGG+GGG | - | chr5.4:54462406-54462425 | MS.gene88733:CDS | 35.0% | |
| GGAGGAGTTTATTAAAAAGG+GGG | - | chr5.4:54462405-54462424 | MS.gene88733:CDS | 35.0% | |
| GGATGAGAGTGATTATAAGA+AGG | - | chr5.4:54462477-54462496 | MS.gene88733:intron | 35.0% | |
| TTCTCAAGAAAATAGGGTTG+GGG | - | chr5.4:54462185-54462204 | MS.gene88733:CDS | 35.0% | |
| !!! | GTGTTTTAAGGTGGTTAGAA+AGG | + | chr5.4:54462064-54462083 | None:intergenic | 35.0% |
| AACTATTGGACCTAAAGGGA+TGG | - | chr5.4:54462441-54462460 | MS.gene88733:intron | 40.0% | |
| ACAATCCAACATTGCACACA+AGG | - | chr5.4:54462094-54462113 | MS.gene88733:CDS | 40.0% | |
| AGCTGGAACATTTGTATCAG+CGG | - | chr5.4:54462347-54462366 | MS.gene88733:CDS | 40.0% | |
| ATTAAAAAGGGGGGGATGAT+TGG | - | chr5.4:54462415-54462434 | MS.gene88733:CDS | 40.0% | |
| ATTCAACGAGACATCACCAT+TGG | - | chr5.4:54462285-54462304 | MS.gene88733:CDS | 40.0% | |
| GGAACAACTATTGGACCTAA+AGG | - | chr5.4:54462436-54462455 | MS.gene88733:intron | 40.0% | |
| GGATAAGAAGTTTGAGCAAG+AGG | - | chr5.4:54462507-54462526 | MS.gene88733:intron | 40.0% | |
| GGGATGATTGGAACAACTAT+TGG | - | chr5.4:54462427-54462446 | MS.gene88733:CDS | 40.0% | |
| TGAAGTTATTGGTGAGCTTC+AGG | - | chr5.4:54462558-54462577 | MS.gene88733:intron | 40.0% | |
| TTCAACGAGACATCACCATT+GGG | - | chr5.4:54462286-54462305 | MS.gene88733:CDS | 40.0% | |
| ! | AGAAGCTTTGGTTTAGGATG+AGG | - | chr5.4:54462533-54462552 | MS.gene88733:intron | 40.0% |
| ! | GGCCGAATAACTACTACTTT+TGG | - | chr5.4:54462206-54462225 | MS.gene88733:CDS | 40.0% |
| ! | TATTGGGCATGTTCTAAACC+TGG | + | chr5.4:54462163-54462182 | None:intergenic | 40.0% |
| !! | AGAAAGGTACGTACCTGTTT+TGG | + | chr5.4:54462048-54462067 | None:intergenic | 40.0% |
| !! | CTTTTGGAAACAAATGGGGA+AGG | - | chr5.4:54462222-54462241 | MS.gene88733:CDS | 40.0% |
| !! | GAAAGGTACGTACCTGTTTT+GGG | + | chr5.4:54462047-54462066 | None:intergenic | 40.0% |
| ATTGGTGAGCTTCAGGAGAA+GGG | - | chr5.4:54462565-54462584 | MS.gene88733:CDS | 45.0% | |
| GAAACAAATGGGGAAGGCTT+TGG | - | chr5.4:54462228-54462247 | MS.gene88733:CDS | 45.0% | |
| GAACACCTTGTGTGCAATGT+TGG | + | chr5.4:54462102-54462121 | None:intergenic | 45.0% | |
| GCATGCACATATGCAGTGAT+GGG | - | chr5.4:54462250-54462269 | MS.gene88733:CDS | 45.0% | |
| TATTGGACCTAAAGGGATGG+TGG | - | chr5.4:54462444-54462463 | MS.gene88733:intron | 45.0% | |
| TATTGGTGAGCTTCAGGAGA+AGG | - | chr5.4:54462564-54462583 | MS.gene88733:CDS | 45.0% | |
| TCATTCTCCACCATCCCTTT+AGG | + | chr5.4:54462454-54462473 | None:intergenic | 45.0% | |
| TCCAGCTCTTTCTCTTCCTT+CGG | + | chr5.4:54462334-54462353 | None:intergenic | 45.0% | |
| TCTTTCTCTTCCTTCGGCAT+TGG | + | chr5.4:54462328-54462347 | None:intergenic | 45.0% | |
| ! | AGGCTCAGAAGCTTTGGTTT+AGG | - | chr5.4:54462527-54462546 | MS.gene88733:intron | 45.0% |
| !! | AGGGATGGTGGAGAATGATA+TGG | - | chr5.4:54462456-54462475 | MS.gene88733:intron | 45.0% |
| !! | GTTTCGAAGAAGCGTTTACG+AGG | + | chr5.4:54462017-54462036 | None:intergenic | 45.0% |
| !!! | TACGTACCTGTTTTGGGTTG+CGG | + | chr5.4:54462041-54462060 | None:intergenic | 45.0% |
| !! | ATAAAATGTAAAAAATTATT+GGG | + | chr5.4:54462139-54462158 | None:intergenic | 5.0% |
| ACGAGACATCACCATTGGGT+TGG | - | chr5.4:54462290-54462309 | MS.gene88733:CDS | 50.0% | |
| AGCAAGAGGCTCAGAAGCTT+TGG | - | chr5.4:54462521-54462540 | MS.gene88733:intron | 50.0% | |
| GAAACACCGCAACCCAAAAC+AGG | - | chr5.4:54462032-54462051 | MS.gene88733:CDS | 50.0% | |
| GGCATGCACATATGCAGTGA+TGG | - | chr5.4:54462249-54462268 | MS.gene88733:CDS | 50.0% | |
| TTCAACGTCCACCAACCCAA+TGG | + | chr5.4:54462304-54462323 | None:intergenic | 50.0% | |
| !! | AGACATCACCATTGGGTTGG+TGG | - | chr5.4:54462293-54462312 | MS.gene88733:CDS | 50.0% |
| !! | CACATATGCAGTGATGGGTG+CGG | - | chr5.4:54462255-54462274 | MS.gene88733:CDS | 50.0% |
| GACGTTGAAGCCAATGCCGA+AGG | - | chr5.4:54462315-54462334 | MS.gene88733:CDS | 55.0% | |
| GCCGAAGGAAGAGAAAGAGC+TGG | - | chr5.4:54462330-54462349 | MS.gene88733:CDS | 55.0% | |
| TCCATAGCTTCCTCGTCTCC+CGG | + | chr5.4:54462388-54462407 | None:intergenic | 55.0% | |
| CGGCGTGAGTTTCCGTATCC+GGG | - | chr5.4:54462367-54462386 | MS.gene88733:CDS | 60.0% | |
| GCGGCGTGAGTTTCCGTATC+CGG | - | chr5.4:54462366-54462385 | MS.gene88733:CDS | 60.0% | |
| GCTTCCTCGTCTCCCGGATA+CGG | + | chr5.4:54462382-54462401 | None:intergenic | 60.0% | |
| GGGAGACGAGGAAGCTATGG+AGG | - | chr5.4:54462387-54462406 | MS.gene88733:CDS | 60.0% | |
| GTTTCCGTATCCGGGAGACG+AGG | - | chr5.4:54462375-54462394 | MS.gene88733:CDS | 60.0% | |
| TCCGGGAGACGAGGAAGCTA+TGG | - | chr5.4:54462384-54462403 | MS.gene88733:CDS | 60.0% | |
| !! | ATGCAGTGATGGGTGCGGCG+TGG | - | chr5.4:54462260-54462279 | MS.gene88733:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 54462011 | 54462606 | 54462011 | ID=MS.gene88733 |
| chr5.4 | mRNA | 54462011 | 54462606 | 54462011 | ID=MS.gene88733.t1;Parent=MS.gene88733 |
| chr5.4 | exon | 54462564 | 54462606 | 54462564 | ID=MS.gene88733.t1.exon1;Parent=MS.gene88733.t1 |
| chr5.4 | CDS | 54462564 | 54462606 | 54462564 | ID=cds.MS.gene88733.t1;Parent=MS.gene88733.t1 |
| chr5.4 | exon | 54462011 | 54462453 | 54462011 | ID=MS.gene88733.t1.exon2;Parent=MS.gene88733.t1 |
| chr5.4 | CDS | 54462011 | 54462453 | 54462011 | ID=cds.MS.gene88733.t1;Parent=MS.gene88733.t1 |
| Gene Sequence |
| Protein sequence |
