Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92558.t1 | XP_013454932.1 | 98.2 | 225 | 4 | 0 | 1 | 225 | 1 | 225 | 1.40E-123 | 452.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92558.t1 | P32110 | 66.1 | 224 | 75 | 1 | 1 | 223 | 1 | 224 | 2.6e-86 | 319.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92558.t1 | B7FMM3 | 98.2 | 225 | 4 | 0 | 1 | 225 | 1 | 225 | 1.0e-123 | 452.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene92558 | 0.804724 | 1.88E-49 | -1.69E-46 |
| MS.gene049488 | MS.gene92558 | 0.810987 | 8.77E-51 | -1.69E-46 |
| MS.gene05081 | MS.gene92558 | 0.801297 | 9.62E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene92558 | 0.813417 | 2.59E-51 | -1.69E-46 |
| MS.gene051255 | MS.gene92558 | 0.80023 | 1.59E-48 | -1.69E-46 |
| MS.gene051858 | MS.gene92558 | 0.804944 | 1.69E-49 | -1.69E-46 |
| MS.gene052322 | MS.gene92558 | 0.805316 | 1.42E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene92558 | 0.813393 | 2.62E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene92558 | 0.816524 | 5.29E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene92558 | 0.800234 | 1.59E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene92558 | 0.831445 | 1.66E-55 | -1.69E-46 |
| MS.gene052870 | MS.gene92558 | 0.817632 | 2.98E-52 | -1.69E-46 |
| MS.gene052872 | MS.gene92558 | 0.815353 | 9.66E-52 | -1.69E-46 |
| MS.gene053758 | MS.gene92558 | 0.846965 | 1.56E-59 | -1.69E-46 |
| MS.gene055206 | MS.gene92558 | 0.806757 | 7.04E-50 | -1.69E-46 |
| MS.gene055412 | MS.gene92558 | 0.800049 | 1.73E-48 | -1.69E-46 |
| MS.gene056511 | MS.gene92558 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene92558 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene92558 | 0.805204 | 1.49E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene92558 | 0.802581 | 5.24E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene92558 | 0.833696 | 4.60E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene92558 | 0.807238 | 5.57E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene92558 | 0.800416 | 1.46E-48 | -1.69E-46 |
| MS.gene059902 | MS.gene92558 | 0.800565 | 1.36E-48 | -1.69E-46 |
| MS.gene060469 | MS.gene92558 | 0.82384 | 1.12E-53 | -1.69E-46 |
| MS.gene060818 | MS.gene92558 | 0.823966 | 1.04E-53 | -1.69E-46 |
| MS.gene060837 | MS.gene92558 | 0.819798 | 9.61E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92558.t1 | MTR_4g019790 | 98.222 | 225 | 4 | 0 | 1 | 225 | 1 | 225 | 1.35e-162 | 448 |
| MS.gene92558.t1 | MTR_7g065720 | 65.158 | 221 | 76 | 1 | 1 | 220 | 1 | 221 | 4.21e-105 | 303 |
| MS.gene92558.t1 | MTR_7g065660 | 61.778 | 225 | 86 | 0 | 1 | 225 | 1 | 225 | 2.32e-104 | 301 |
| MS.gene92558.t1 | MTR_7g065590 | 61.435 | 223 | 86 | 0 | 1 | 223 | 1 | 223 | 9.53e-104 | 299 |
| MS.gene92558.t1 | MTR_7g065740 | 62.389 | 226 | 84 | 1 | 1 | 225 | 1 | 226 | 1.26e-103 | 299 |
| MS.gene92558.t1 | MTR_7g065290 | 63.470 | 219 | 80 | 0 | 1 | 219 | 1 | 219 | 1.46e-103 | 298 |
| MS.gene92558.t1 | MTR_7g065600 | 61.883 | 223 | 85 | 0 | 1 | 223 | 1 | 223 | 3.95e-103 | 298 |
| MS.gene92558.t1 | MTR_7g065750 | 64.159 | 226 | 80 | 1 | 1 | 225 | 1 | 226 | 4.22e-102 | 295 |
| MS.gene92558.t1 | MTR_7g065270 | 61.290 | 217 | 84 | 0 | 1 | 217 | 1 | 217 | 1.61e-96 | 281 |
| MS.gene92558.t1 | MTR_7g065265 | 60.829 | 217 | 85 | 0 | 1 | 217 | 1 | 217 | 5.01e-95 | 277 |
| MS.gene92558.t1 | MTR_7g065640 | 58.222 | 225 | 76 | 2 | 1 | 225 | 1 | 207 | 4.89e-94 | 274 |
| MS.gene92558.t1 | MTR_4g019780 | 60.094 | 213 | 83 | 2 | 4 | 215 | 5 | 216 | 6.45e-94 | 274 |
| MS.gene92558.t1 | MTR_7g065230 | 58.744 | 223 | 87 | 1 | 1 | 223 | 1 | 218 | 7.03e-94 | 274 |
| MS.gene92558.t1 | MTR_7g065630 | 60.000 | 225 | 86 | 1 | 1 | 225 | 19 | 239 | 2.43e-93 | 273 |
| MS.gene92558.t1 | MTR_7g065260 | 60.268 | 224 | 88 | 1 | 1 | 223 | 8 | 231 | 7.06e-91 | 267 |
| MS.gene92558.t1 | MTR_7g065710 | 57.005 | 207 | 85 | 1 | 8 | 214 | 5 | 207 | 3.89e-81 | 242 |
| MS.gene92558.t1 | MTR_7g065700 | 60.748 | 214 | 80 | 1 | 1 | 214 | 1 | 210 | 5.06e-80 | 239 |
| MS.gene92558.t1 | MTR_7g065680 | 56.444 | 225 | 94 | 1 | 1 | 225 | 1 | 221 | 2.44e-79 | 237 |
| MS.gene92558.t1 | MTR_1g090150 | 46.606 | 221 | 113 | 3 | 7 | 224 | 3 | 221 | 9.65e-66 | 203 |
| MS.gene92558.t1 | MTR_6g080440 | 45.714 | 210 | 112 | 2 | 5 | 213 | 1 | 209 | 2.10e-65 | 202 |
| MS.gene92558.t1 | MTR_8g087425 | 46.188 | 223 | 112 | 3 | 7 | 225 | 5 | 223 | 2.44e-65 | 202 |
| MS.gene92558.t1 | MTR_3g099757 | 44.889 | 225 | 120 | 3 | 1 | 224 | 1 | 222 | 6.88e-63 | 195 |
| MS.gene92558.t1 | MTR_1g115195 | 46.977 | 215 | 109 | 3 | 6 | 216 | 2 | 215 | 2.47e-61 | 191 |
| MS.gene92558.t1 | MTR_8g087410 | 44.690 | 226 | 122 | 3 | 1 | 225 | 1 | 224 | 9.37e-61 | 190 |
| MS.gene92558.t1 | MTR_4g059730 | 44.811 | 212 | 113 | 2 | 7 | 215 | 4 | 214 | 1.04e-59 | 187 |
| MS.gene92558.t1 | MTR_5g040430 | 40.991 | 222 | 123 | 3 | 1 | 215 | 1 | 221 | 6.80e-58 | 183 |
| MS.gene92558.t1 | MTR_1g090060 | 41.778 | 225 | 124 | 3 | 6 | 224 | 2 | 225 | 5.30e-56 | 178 |
| MS.gene92558.t1 | MTR_1g090070 | 41.892 | 222 | 126 | 1 | 6 | 224 | 8 | 229 | 7.60e-56 | 177 |
| MS.gene92558.t1 | MTR_1g090090 | 42.857 | 210 | 116 | 2 | 6 | 212 | 2 | 210 | 1.93e-54 | 174 |
| MS.gene92558.t1 | MTR_1g090100 | 42.180 | 211 | 118 | 2 | 8 | 215 | 3 | 212 | 8.32e-53 | 169 |
| MS.gene92558.t1 | MTR_5g037380 | 41.892 | 222 | 121 | 2 | 7 | 220 | 5 | 226 | 7.41e-51 | 165 |
| MS.gene92558.t1 | MTR_8g061950 | 40.183 | 219 | 123 | 4 | 5 | 215 | 3 | 221 | 6.38e-48 | 157 |
| MS.gene92558.t1 | MTR_2g070150 | 41.872 | 203 | 113 | 4 | 7 | 207 | 4 | 203 | 2.25e-47 | 155 |
| MS.gene92558.t1 | MTR_2g070130 | 43.902 | 205 | 110 | 5 | 7 | 209 | 4 | 205 | 1.05e-46 | 154 |
| MS.gene92558.t1 | MTR_2g070140 | 41.753 | 194 | 108 | 4 | 21 | 212 | 2 | 192 | 3.10e-46 | 152 |
| MS.gene92558.t1 | MTR_2g070200 | 40.796 | 201 | 115 | 3 | 7 | 205 | 4 | 202 | 3.93e-43 | 145 |
| MS.gene92558.t1 | MTR_3g467420 | 39.048 | 210 | 123 | 4 | 5 | 212 | 7 | 213 | 7.98e-43 | 144 |
| MS.gene92558.t1 | MTR_2g070180 | 39.901 | 203 | 117 | 4 | 7 | 207 | 4 | 203 | 3.57e-42 | 142 |
| MS.gene92558.t1 | MTR_2g070210 | 40.952 | 210 | 117 | 5 | 7 | 212 | 4 | 210 | 1.17e-41 | 141 |
| MS.gene92558.t1 | MTR_2g070120 | 39.524 | 210 | 120 | 6 | 7 | 213 | 4 | 209 | 7.25e-41 | 139 |
| MS.gene92558.t1 | MTR_2g070060 | 39.171 | 217 | 124 | 5 | 1 | 215 | 1 | 211 | 1.26e-40 | 138 |
| MS.gene92558.t1 | MTR_4g124130 | 43.979 | 191 | 99 | 6 | 5 | 191 | 2 | 188 | 1.29e-40 | 138 |
| MS.gene92558.t1 | MTR_8g056940 | 38.462 | 208 | 123 | 4 | 7 | 212 | 4 | 208 | 1.56e-40 | 138 |
| MS.gene92558.t1 | MTR_3g467430 | 37.441 | 211 | 127 | 4 | 4 | 212 | 6 | 213 | 3.97e-40 | 137 |
| MS.gene92558.t1 | MTR_5g076900 | 40.097 | 207 | 119 | 4 | 8 | 212 | 7 | 210 | 1.11e-39 | 136 |
| MS.gene92558.t1 | MTR_2g070070 | 41.014 | 217 | 120 | 5 | 1 | 215 | 1 | 211 | 8.29e-39 | 134 |
| MS.gene92558.t1 | MTR_0186s0030 | 46.715 | 137 | 71 | 2 | 7 | 142 | 4 | 139 | 3.99e-35 | 122 |
| MS.gene92558.t1 | MTR_2g070110 | 39.189 | 222 | 126 | 6 | 1 | 220 | 1 | 215 | 2.06e-34 | 122 |
| MS.gene92558.t1 | MTR_2g072120 | 50.769 | 65 | 31 | 1 | 51 | 114 | 2 | 66 | 3.24e-17 | 73.9 |
| MS.gene92558.t1 | MTR_5g037390 | 39.726 | 73 | 41 | 1 | 31 | 100 | 11 | 83 | 2.88e-11 | 58.5 |
| MS.gene92558.t1 | MTR_1g067180 | 27.354 | 223 | 136 | 7 | 6 | 217 | 26 | 233 | 4.17e-11 | 61.2 |
| MS.gene92558.t1 | MTR_1g067180 | 26.636 | 214 | 131 | 6 | 15 | 217 | 32 | 230 | 9.16e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92558.t1 | AT3G09270 | 49.776 | 223 | 108 | 4 | 3 | 222 | 2 | 223 | 5.55e-79 | 236 |
| MS.gene92558.t1 | AT2G29420 | 46.188 | 223 | 118 | 2 | 3 | 224 | 5 | 226 | 9.66e-73 | 221 |
| MS.gene92558.t1 | AT2G29490 | 48.804 | 209 | 104 | 3 | 1 | 207 | 1 | 208 | 5.02e-63 | 196 |
| MS.gene92558.t1 | AT2G29480 | 49.289 | 211 | 100 | 4 | 1 | 207 | 1 | 208 | 1.09e-61 | 192 |
| MS.gene92558.t1 | AT2G29460 | 44.053 | 227 | 120 | 4 | 1 | 223 | 1 | 224 | 7.53e-59 | 185 |
| MS.gene92558.t1 | AT2G29450 | 46.117 | 206 | 109 | 2 | 4 | 207 | 3 | 208 | 4.24e-58 | 183 |
| MS.gene92558.t1 | AT2G29470 | 43.810 | 210 | 114 | 4 | 1 | 207 | 1 | 209 | 8.29e-55 | 175 |
| MS.gene92558.t1 | AT2G29440 | 41.553 | 219 | 125 | 3 | 7 | 223 | 6 | 223 | 3.49e-54 | 173 |
| MS.gene92558.t1 | AT1G59700 | 43.723 | 231 | 121 | 4 | 4 | 225 | 3 | 233 | 1.02e-52 | 170 |
| MS.gene92558.t1 | AT1G59670 | 44.091 | 220 | 115 | 3 | 3 | 214 | 2 | 221 | 1.18e-52 | 170 |
| MS.gene92558.t1 | AT1G10370 | 41.475 | 217 | 120 | 2 | 5 | 214 | 3 | 219 | 1.27e-50 | 164 |
| MS.gene92558.t1 | AT1G69920 | 40.183 | 219 | 123 | 4 | 8 | 218 | 35 | 253 | 1.25e-48 | 160 |
| MS.gene92558.t1 | AT1G74590 | 38.559 | 236 | 127 | 5 | 1 | 224 | 1 | 230 | 1.83e-48 | 159 |
| MS.gene92558.t1 | AT1G27130 | 38.073 | 218 | 127 | 3 | 8 | 217 | 7 | 224 | 2.10e-48 | 159 |
| MS.gene92558.t1 | AT5G62480 | 38.009 | 221 | 126 | 3 | 5 | 215 | 6 | 225 | 7.64e-48 | 157 |
| MS.gene92558.t1 | AT1G78380 | 42.991 | 214 | 114 | 5 | 1 | 212 | 1 | 208 | 2.17e-47 | 155 |
| MS.gene92558.t1 | AT1G78340 | 41.346 | 208 | 117 | 4 | 7 | 212 | 4 | 208 | 9.80e-47 | 154 |
| MS.gene92558.t1 | AT1G78370 | 41.463 | 205 | 115 | 4 | 10 | 212 | 7 | 208 | 2.34e-46 | 153 |
| MS.gene92558.t1 | AT1G69930 | 37.037 | 216 | 130 | 2 | 8 | 217 | 13 | 228 | 2.36e-46 | 154 |
| MS.gene92558.t1 | AT1G78320 | 42.029 | 207 | 113 | 5 | 7 | 210 | 4 | 206 | 1.78e-45 | 151 |
| MS.gene92558.t1 | AT1G17170 | 41.509 | 212 | 118 | 5 | 7 | 216 | 4 | 211 | 6.58e-45 | 149 |
| MS.gene92558.t1 | AT1G10360 | 38.140 | 215 | 126 | 3 | 7 | 214 | 5 | 219 | 2.01e-44 | 148 |
| MS.gene92558.t1 | AT1G27140 | 38.073 | 218 | 126 | 3 | 8 | 216 | 7 | 224 | 5.34e-43 | 145 |
| MS.gene92558.t1 | AT1G17180 | 39.904 | 208 | 120 | 4 | 7 | 212 | 4 | 208 | 8.21e-43 | 144 |
| MS.gene92558.t1 | AT1G53680 | 39.130 | 207 | 121 | 4 | 1 | 204 | 1 | 205 | 1.76e-42 | 143 |
| MS.gene92558.t1 | AT1G78360 | 39.048 | 210 | 122 | 5 | 6 | 212 | 3 | 209 | 1.72e-39 | 135 |
| MS.gene92558.t1 | AT1G17190 | 39.720 | 214 | 122 | 5 | 1 | 212 | 1 | 209 | 5.86e-39 | 134 |
| MS.gene92558.t1 | AT3G43800 | 38.725 | 204 | 116 | 6 | 7 | 204 | 5 | 205 | 1.15e-38 | 134 |
| MS.gene92558.t1 | AT1G78320 | 35.922 | 206 | 100 | 5 | 7 | 210 | 4 | 179 | 4.63e-30 | 110 |
| MS.gene92558.t1 | AT5G62480 | 30.909 | 220 | 117 | 4 | 5 | 215 | 6 | 199 | 1.94e-29 | 109 |
| MS.gene92558.t1 | AT5G62480 | 30.909 | 220 | 116 | 4 | 5 | 215 | 6 | 198 | 3.37e-29 | 108 |
Find 51 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTCCTACTTAGCTTTC+TGG | 0.061163 | 4.2:-82442455 | MS.gene92558:CDS |
| GTTCATTCTCAAGAAATTCT+AGG | 0.255826 | 4.2:+82442526 | None:intergenic |
| GAACTCAAGCACAAATTCTT+TGG | 0.284917 | 4.2:-82442507 | MS.gene92558:CDS |
| AGAAAGCCTTGGCTCGATTT+TGG | 0.296277 | 4.2:-82443065 | MS.gene92558:CDS |
| TAAGGTTGTTGAGGGAAAAT+AGG | 0.325386 | 4.2:+82443090 | None:intergenic |
| CAGGCACCTTCTTGTGAATA+GGG | 0.332659 | 4.2:+82443190 | None:intergenic |
| CGAGCCAAGGCTTTCTCATA+AGG | 0.351441 | 4.2:+82443072 | None:intergenic |
| CAAGGAGAAGTTCAATTGTT+AGG | 0.356671 | 4.2:-82443330 | MS.gene92558:CDS |
| AATTTCCTAAGCTCTATAAA+TGG | 0.358102 | 4.2:-82442383 | MS.gene92558:CDS |
| ACAGGCACCTTCTTGTGAAT+AGG | 0.360494 | 4.2:+82443189 | None:intergenic |
| AAGCCGAGGAGTCCATCTTT+TGG | 0.362601 | 4.2:+82442315 | None:intergenic |
| TTGAACTTCTCCTTGATCAT+TGG | 0.365725 | 4.2:+82443338 | None:intergenic |
| ATTTGCACCCTTCGAACAAA+TGG | 0.368308 | 4.2:+82443294 | None:intergenic |
| GGGAGATTCTCTTTCACAAT+AGG | 0.372294 | 4.2:+82442339 | None:intergenic |
| CTTACATTGAAGAAAATCTA+AGG | 0.391102 | 4.2:-82443242 | MS.gene92558:CDS |
| CATGCAGCCTTTGATATTGC+TGG | 0.393495 | 4.2:+82442600 | None:intergenic |
| ATGTTTCATCAATGTATTCA+AGG | 0.424371 | 4.2:+82443124 | None:intergenic |
| CTTTCTCATAAGGTTGTTGA+GGG | 0.434221 | 4.2:+82443082 | None:intergenic |
| CAAATTGCTCTAAAACTAAA+GGG | 0.440385 | 4.2:-82443276 | MS.gene92558:CDS |
| GCAAATTGCTCTAAAACTAA+AGG | 0.446586 | 4.2:-82443277 | MS.gene92558:CDS |
| GAGAATCTCCCTCCAAAAGA+TGG | 0.451824 | 4.2:-82442327 | MS.gene92558:CDS |
| GAAGTTCAATTGTTAGGTGT+AGG | 0.453999 | 4.2:-82443324 | MS.gene92558:CDS |
| TGACTCCATTTATAGAGCTT+AGG | 0.457524 | 4.2:+82442378 | None:intergenic |
| TTCATTCTCAAGAAATTCTA+GGG | 0.459545 | 4.2:+82442527 | None:intergenic |
| GTTCAATTGTTAGGTGTAGG+AGG | 0.480588 | 4.2:-82443321 | MS.gene92558:CDS |
| TTTGAAATGGCCAATGATCA+AGG | 0.497609 | 4.2:-82443348 | None:intergenic |
| AGGAAGCCAGAAAGCTAAGT+AGG | 0.500826 | 4.2:+82442449 | None:intergenic |
| TTTGCACCCTTCGAACAAAT+GGG | 0.505097 | 4.2:+82443295 | None:intergenic |
| GATCACAAGGGACTCAGCTA+AGG | 0.517350 | 4.2:+82443146 | None:intergenic |
| AACAAAGAAGAACGTGAGAA+AGG | 0.523741 | 4.2:-82442570 | MS.gene92558:CDS |
| GCTTTCTCATAAGGTTGTTG+AGG | 0.531026 | 4.2:+82443081 | None:intergenic |
| CTCAAGCACAAATTCTTTGG+TGG | 0.534226 | 4.2:-82442504 | MS.gene92558:CDS |
| CTGTGATTGAAGAAGCTGTA+GGG | 0.541030 | 4.2:-82442428 | MS.gene92558:CDS |
| CAGCCTTTGATATTGCTGGC+AGG | 0.552662 | 4.2:+82442604 | None:intergenic |
| GAGGAAGCCCATTTGTTCGA+AGG | 0.559814 | 4.2:-82443302 | MS.gene92558:CDS |
| CAATGTATTCAAGGATCACA+AGG | 0.561254 | 4.2:+82443133 | None:intergenic |
| CCTCCAAAAGATGGACTCCT+CGG | 0.566098 | 4.2:-82442318 | MS.gene92558:CDS |
| GTCGAGGTTCATTGATGACA+AGG | 0.566263 | 4.2:-82443043 | MS.gene92558:intron |
| TTGAATACATTGATGAAACA+TGG | 0.571451 | 4.2:-82443122 | MS.gene92558:CDS |
| AGGAAGCCCATTTGTTCGAA+GGG | 0.574450 | 4.2:-82443301 | MS.gene92558:CDS |
| ACAACCTTATGAGAAAGCCT+TGG | 0.593342 | 4.2:-82443076 | MS.gene92558:CDS |
| CCTGTGATTGAAGAAGCTGT+AGG | 0.598472 | 4.2:-82442429 | MS.gene92558:CDS |
| ATCACAAGGGACTCAGCTAA+GGG | 0.601842 | 4.2:+82443147 | None:intergenic |
| CAGCAATATCAAAGGCTGCA+TGG | 0.606136 | 4.2:-82442599 | MS.gene92558:CDS |
| CCTACAGCTTCTTCAATCAC+AGG | 0.610321 | 4.2:+82442429 | None:intergenic |
| GTATAACCCTATTCACAAGA+AGG | 0.611526 | 4.2:-82443196 | MS.gene92558:CDS |
| TGAATACATTGATGAAACAT+GGG | 0.611722 | 4.2:-82443121 | MS.gene92558:CDS |
| TGGAGAAAACATTAGCATTG+TGG | 0.621375 | 4.2:-82442484 | MS.gene92558:CDS |
| GATCCTGCCAGCAATATCAA+AGG | 0.632199 | 4.2:-82442607 | MS.gene92558:CDS |
| CCTCGACCAAAATCGAGCCA+AGG | 0.642750 | 4.2:+82443059 | None:intergenic |
| AATGTATTCAAGGATCACAA+GGG | 0.737483 | 4.2:+82443134 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAATATATTATGATGA+TGG | + | chr4.2:82442892-82442911 | None:intergenic | 10.0% |
| !!! | AATTTCACATTATTTTTAAA+AGG | - | chr4.2:82442757-82442776 | MS.gene92558:intron | 10.0% |
| !!! | TGTTGTTTCATAATATTATT+AGG | + | chr4.2:82442963-82442982 | None:intergenic | 15.0% |
| !!! | TTTTTTGTTGAAAAGATATT+AGG | + | chr4.2:82442928-82442947 | None:intergenic | 15.0% |
| !!! | TAATGTGAAATTTTTGACAT+TGG | + | chr4.2:82442749-82442768 | None:intergenic | 20.0% |
| ! | AATTTCCTAAGCTCTATAAA+TGG | - | chr4.2:82443231-82443250 | MS.gene92558:CDS | 25.0% |
| ! | ATGTTTCATCAATGTATTCA+AGG | + | chr4.2:82442493-82442512 | None:intergenic | 25.0% |
| ! | CTTACATTGAAGAAAATCTA+AGG | - | chr4.2:82442372-82442391 | MS.gene92558:CDS | 25.0% |
| ! | TGAATACATTGATGAAACAT+GGG | - | chr4.2:82442493-82442512 | MS.gene92558:CDS | 25.0% |
| ! | TTACCATTATGAACAAAAAC+AGG | + | chr4.2:82442446-82442465 | None:intergenic | 25.0% |
| ! | TTCATTCTCAAGAAATTCTA+GGG | + | chr4.2:82443090-82443109 | None:intergenic | 25.0% |
| ! | TTGAATACATTGATGAAACA+TGG | - | chr4.2:82442492-82442511 | MS.gene92558:CDS | 25.0% |
| !! | TTCATGATGTTTTATACACA+GGG | + | chr4.2:82442828-82442847 | None:intergenic | 25.0% |
| !! | TTTCATGATGTTTTATACAC+AGG | + | chr4.2:82442829-82442848 | None:intergenic | 25.0% |
| !!! | CAAATTGCTCTAAAACTAAA+GGG | - | chr4.2:82442338-82442357 | MS.gene92558:CDS | 25.0% |
| AATGTATTCAAGGATCACAA+GGG | + | chr4.2:82442483-82442502 | None:intergenic | 30.0% | |
| GTTCATTCTCAAGAAATTCT+AGG | + | chr4.2:82443091-82443110 | None:intergenic | 30.0% | |
| TGCAATTCATCAATCATCAT+AGG | + | chr4.2:82442868-82442887 | None:intergenic | 30.0% | |
| !! | GCAAATTGCTCTAAAACTAA+AGG | - | chr4.2:82442337-82442356 | MS.gene92558:CDS | 30.0% |
| AACAAAGAAGAACGTGAGAA+AGG | - | chr4.2:82443044-82443063 | MS.gene92558:CDS | 35.0% | |
| CAAGGAGAAGTTCAATTGTT+AGG | - | chr4.2:82442284-82442303 | MS.gene92558:CDS | 35.0% | |
| CAATGTATTCAAGGATCACA+AGG | + | chr4.2:82442484-82442503 | None:intergenic | 35.0% | |
| CTTTAGGTCTCATCATATGT+TGG | - | chr4.2:82442797-82442816 | MS.gene92558:intron | 35.0% | |
| CTTTCTCATAAGGTTGTTGA+GGG | + | chr4.2:82442535-82442554 | None:intergenic | 35.0% | |
| GAACTCAAGCACAAATTCTT+TGG | - | chr4.2:82443107-82443126 | MS.gene92558:CDS | 35.0% | |
| GAAGTTCAATTGTTAGGTGT+AGG | - | chr4.2:82442290-82442309 | MS.gene92558:CDS | 35.0% | |
| GTATAACCCTATTCACAAGA+AGG | - | chr4.2:82442418-82442437 | MS.gene92558:CDS | 35.0% | |
| TAAGGTTGTTGAGGGAAAAT+AGG | + | chr4.2:82442527-82442546 | None:intergenic | 35.0% | |
| TGACTCCATTTATAGAGCTT+AGG | + | chr4.2:82443239-82443258 | None:intergenic | 35.0% | |
| TGGAGAAAACATTAGCATTG+TGG | - | chr4.2:82443130-82443149 | MS.gene92558:CDS | 35.0% | |
| TTGAACTTCTCCTTGATCAT+TGG | + | chr4.2:82442279-82442298 | None:intergenic | 35.0% | |
| ! | CTCGTCTAAATTTTCGCTTT+AGG | - | chr4.2:82442781-82442800 | MS.gene92558:intron | 35.0% |
| !!! | GTGCCTGTTTTTGTTCATAA+TGG | - | chr4.2:82442440-82442459 | MS.gene92558:CDS | 35.0% |
| ACAACCTTATGAGAAAGCCT+TGG | - | chr4.2:82442538-82442557 | MS.gene92558:CDS | 40.0% | |
| ACGATATACCACCATGATTC+TGG | + | chr4.2:82442663-82442682 | None:intergenic | 40.0% | |
| ATCGAGATTTGAAAAAGCCG+AGG | + | chr4.2:82443316-82443335 | None:intergenic | 40.0% | |
| ATTTGCACCCTTCGAACAAA+TGG | + | chr4.2:82442323-82442342 | None:intergenic | 40.0% | |
| CTCAAGCACAAATTCTTTGG+TGG | - | chr4.2:82443110-82443129 | MS.gene92558:CDS | 40.0% | |
| CTGTGATTGAAGAAGCTGTA+GGG | - | chr4.2:82443186-82443205 | MS.gene92558:CDS | 40.0% | |
| GCTTTCTCATAAGGTTGTTG+AGG | + | chr4.2:82442536-82442555 | None:intergenic | 40.0% | |
| GGGAGATTCTCTTTCACAAT+AGG | + | chr4.2:82443278-82443297 | None:intergenic | 40.0% | |
| GTTCAATTGTTAGGTGTAGG+AGG | - | chr4.2:82442293-82442312 | MS.gene92558:CDS | 40.0% | |
| TGATGTATCTGCCAGAATCA+TGG | - | chr4.2:82442649-82442668 | MS.gene92558:intron | 40.0% | |
| TTTGCACCCTTCGAACAAAT+GGG | + | chr4.2:82442322-82442341 | None:intergenic | 40.0% | |
| ! | TGATGTTTTATACACAGGGC+CGG | + | chr4.2:82442824-82442843 | None:intergenic | 40.0% |
| ACAGGCACCTTCTTGTGAAT+AGG | + | chr4.2:82442428-82442447 | None:intergenic | 45.0% | |
| AGGAAGCCAGAAAGCTAAGT+AGG | + | chr4.2:82443168-82443187 | None:intergenic | 45.0% | |
| AGGAAGCCCATTTGTTCGAA+GGG | - | chr4.2:82442313-82442332 | MS.gene92558:CDS | 45.0% | |
| ATCACAAGGGACTCAGCTAA+GGG | + | chr4.2:82442470-82442489 | None:intergenic | 45.0% | |
| CAGCAATATCAAAGGCTGCA+TGG | - | chr4.2:82443015-82443034 | MS.gene92558:intron | 45.0% | |
| CAGGCACCTTCTTGTGAATA+GGG | + | chr4.2:82442427-82442446 | None:intergenic | 45.0% | |
| CATGCAGCCTTTGATATTGC+TGG | + | chr4.2:82443017-82443036 | None:intergenic | 45.0% | |
| CCTACAGCTTCTTCAATCAC+AGG | + | chr4.2:82443188-82443207 | None:intergenic | 45.0% | |
| CCTGTGATTGAAGAAGCTGT+AGG | - | chr4.2:82443185-82443204 | MS.gene92558:CDS | 45.0% | |
| GAGAATCTCCCTCCAAAAGA+TGG | - | chr4.2:82443287-82443306 | MS.gene92558:CDS | 45.0% | |
| GATCCTGCCAGCAATATCAA+AGG | - | chr4.2:82443007-82443026 | MS.gene92558:intron | 45.0% | |
| GGTCTCATCATATGTTGGAC+CGG | - | chr4.2:82442802-82442821 | MS.gene92558:intron | 45.0% | |
| TGTATCTGCCAGAATCATGG+TGG | - | chr4.2:82442652-82442671 | MS.gene92558:intron | 45.0% | |
| ! | CTGCTTCCTACTTAGCTTTC+TGG | - | chr4.2:82443159-82443178 | MS.gene92558:CDS | 45.0% |
| ! | GTCGAGGTTCATTGATGACA+AGG | - | chr4.2:82442571-82442590 | MS.gene92558:CDS | 45.0% |
| !! | AGAAAGCCTTGGCTCGATTT+TGG | - | chr4.2:82442549-82442568 | MS.gene92558:CDS | 45.0% |
| CAGCCTTTGATATTGCTGGC+AGG | + | chr4.2:82443013-82443032 | None:intergenic | 50.0% | |
| CGAGCCAAGGCTTTCTCATA+AGG | + | chr4.2:82442545-82442564 | None:intergenic | 50.0% | |
| GAGGAAGCCCATTTGTTCGA+AGG | - | chr4.2:82442312-82442331 | MS.gene92558:CDS | 50.0% | |
| GATCACAAGGGACTCAGCTA+AGG | + | chr4.2:82442471-82442490 | None:intergenic | 50.0% | |
| ! | AAGCCGAGGAGTCCATCTTT+TGG | + | chr4.2:82443302-82443321 | None:intergenic | 50.0% |
| ! | CCTCCAAAAGATGGACTCCT+CGG | - | chr4.2:82443296-82443315 | MS.gene92558:CDS | 50.0% |
| ! | CGAGGAGTCCATCTTTTGGA+GGG | + | chr4.2:82443298-82443317 | None:intergenic | 50.0% |
| CCTCGACCAAAATCGAGCCA+AGG | + | chr4.2:82442558-82442577 | None:intergenic | 55.0% | |
| ! | CCGAGGAGTCCATCTTTTGG+AGG | + | chr4.2:82443299-82443318 | None:intergenic | 55.0% |
| !! | CCTTGGCTCGATTTTGGTCG+AGG | - | chr4.2:82442555-82442574 | MS.gene92558:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 82442272 | 82443364 | 82442272 | ID=MS.gene92558 |
| chr4.2 | mRNA | 82442272 | 82443364 | 82442272 | ID=MS.gene92558.t1;Parent=MS.gene92558 |
| chr4.2 | exon | 82443044 | 82443364 | 82443044 | ID=MS.gene92558.t1.exon1;Parent=MS.gene92558.t1 |
| chr4.2 | CDS | 82443044 | 82443364 | 82443044 | ID=cds.MS.gene92558.t1;Parent=MS.gene92558.t1 |
| chr4.2 | exon | 82442272 | 82442628 | 82442272 | ID=MS.gene92558.t1.exon2;Parent=MS.gene92558.t1 |
| chr4.2 | CDS | 82442272 | 82442628 | 82442272 | ID=cds.MS.gene92558.t1;Parent=MS.gene92558.t1 |
| Gene Sequence |
| Protein sequence |
