Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96245.t1 | PNX65784.1 | 85.3 | 75 | 7 | 1 | 1 | 75 | 1 | 71 | 4.20E-24 | 120.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96245.t1 | A0A392T8J3 | 88.0 | 75 | 7 | 1 | 1 | 75 | 1 | 73 | 3.6e-25 | 123.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene96245 | 0.822754 | 2.00E-53 | -1.69E-46 |
| MS.gene050689 | MS.gene96245 | 0.803267 | 3.78E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene96245 | 0.817582 | 3.06E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene96245 | 0.83245 | 9.39E-56 | -1.69E-46 |
| MS.gene050955 | MS.gene96245 | 0.809005 | 2.34E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene96245 | 0.826942 | 2.06E-54 | -1.69E-46 |
| MS.gene051233 | MS.gene96245 | 0.828378 | 9.30E-55 | -1.69E-46 |
| MS.gene051234 | MS.gene96245 | 0.82506 | 5.76E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene96245 | 0.801794 | 7.61E-49 | -1.69E-46 |
| MS.gene052072 | MS.gene96245 | 0.808238 | 3.42E-50 | -1.69E-46 |
| MS.gene052296 | MS.gene96245 | 0.814151 | 1.78E-51 | -1.69E-46 |
| MS.gene052335 | MS.gene96245 | 0.817031 | 4.07E-52 | -1.69E-46 |
| MS.gene052479 | MS.gene96245 | 0.823239 | 1.54E-53 | -1.69E-46 |
| MS.gene053058 | MS.gene96245 | 0.813508 | 2.47E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene96245 | 0.81665 | 4.96E-52 | -1.69E-46 |
| MS.gene056992 | MS.gene96245 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene96245 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene057234 | MS.gene96245 | 0.815631 | 8.37E-52 | -1.69E-46 |
| MS.gene057705 | MS.gene96245 | 0.800727 | 1.26E-48 | -1.69E-46 |
| MS.gene057999 | MS.gene96245 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene058054 | MS.gene96245 | 0.811507 | 6.76E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene96245 | 0.800426 | 1.45E-48 | -1.69E-46 |
| MS.gene058074 | MS.gene96245 | 0.819587 | 1.07E-52 | -1.69E-46 |
| MS.gene059146 | MS.gene96245 | 0.839741 | 1.32E-57 | -1.69E-46 |
| MS.gene06011 | MS.gene96245 | 0.807943 | 3.95E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene96245 | 0.839926 | 1.18E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96245.t1 | MTR_2g019240 | 84.416 | 77 | 3 | 2 | 1 | 75 | 1 | 70 | 4.76e-38 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96245.t1 | AT2G34585 | 76.190 | 42 | 10 | 0 | 1 | 42 | 1 | 42 | 9.45e-19 | 73.2 |
Find 19 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGTTAGGGGCGGGGTTGAG+TGG | 0.375759 | 2.2:+65391611 | None:intergenic |
| TCGTCGTCGGGATCGTAATC+AGG | 0.386527 | 2.2:+65391653 | None:intergenic |
| TTGACGACCTTGTTCGAGGC+AGG | 0.394231 | 2.2:+65391697 | None:intergenic |
| GCAGTGGTAGCAGTGATTCT+AGG | 0.395537 | 2.2:-65391749 | MS.gene96245:CDS |
| GCAGCGGTGGTGGAGGCGAC+GGG | 0.399580 | 2.2:+65391587 | None:intergenic |
| AGCAGCGGTGGTGGAGGCGA+CGG | 0.425781 | 2.2:+65391586 | None:intergenic |
| AGGATTGAGATTGCGATCAA+TGG | 0.439079 | 2.2:+65391673 | None:intergenic |
| ACTGCGTTGTCTTCGTCGTC+GGG | 0.450375 | 2.2:+65391641 | None:intergenic |
| AACTGCGTTGTCTTCGTCGT+CGG | 0.458028 | 2.2:+65391640 | None:intergenic |
| AGTGAATACCACTTATGAAA+CGG | 0.507725 | 2.2:+65391559 | None:intergenic |
| CAGCGGTGGTGGAGGCGACG+GGG | 0.518735 | 2.2:+65391588 | None:intergenic |
| TGGCTTGACGACCTTGTTCG+AGG | 0.522648 | 2.2:+65391693 | None:intergenic |
| GGGATCATGGGAGCAATCAC+CGG | 0.545095 | 2.2:-65391782 | None:intergenic |
| TGACGACCTTGTTCGAGGCA+GGG | 0.551454 | 2.2:+65391698 | None:intergenic |
| TGTAAACCCTGCCTCGAACA+AGG | 0.577165 | 2.2:-65391704 | MS.gene96245:CDS |
| TACCACTGCTATCACTGCAC+CGG | 0.586478 | 2.2:+65391763 | None:intergenic |
| AGCGGTGGTGGAGGCGACGG+GGG | 0.608456 | 2.2:+65391589 | None:intergenic |
| TGGTAGCAGTGATTCTAGGT+TGG | 0.618694 | 2.2:-65391745 | MS.gene96245:CDS |
| CACCGGTGCAGTGATAGCAG+TGG | 0.664884 | 2.2:-65391765 | MS.gene96245:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGAAACGGTTTTAGCAG+CGG | + | chr2.2:65391779-65391798 | None:intergenic | 35.0% |
| AGGATTGAGATTGCGATCAA+TGG | + | chr2.2:65391677-65391696 | None:intergenic | 40.0% | |
| TGGTAGCAGTGATTCTAGGT+TGG | - | chr2.2:65391602-65391621 | MS.gene96245:CDS | 45.0% | |
| AACTGCGTTGTCTTCGTCGT+CGG | + | chr2.2:65391710-65391729 | None:intergenic | 50.0% | |
| GCAGTGGTAGCAGTGATTCT+AGG | - | chr2.2:65391598-65391617 | MS.gene96245:CDS | 50.0% | |
| TACCACTGCTATCACTGCAC+CGG | + | chr2.2:65391587-65391606 | None:intergenic | 50.0% | |
| TGTAAACCCTGCCTCGAACA+AGG | - | chr2.2:65391643-65391662 | MS.gene96245:CDS | 50.0% | |
| !! | TGAAACGGTTTTAGCAGCGG+TGG | + | chr2.2:65391776-65391795 | None:intergenic | 50.0% |
| ACTGCGTTGTCTTCGTCGTC+GGG | + | chr2.2:65391709-65391728 | None:intergenic | 55.0% | |
| TCGTCGTCGGGATCGTAATC+AGG | + | chr2.2:65391697-65391716 | None:intergenic | 55.0% | |
| TGACGACCTTGTTCGAGGCA+GGG | + | chr2.2:65391652-65391671 | None:intergenic | 55.0% | |
| TGGCTTGACGACCTTGTTCG+AGG | + | chr2.2:65391657-65391676 | None:intergenic | 55.0% | |
| TTGACGACCTTGTTCGAGGC+AGG | + | chr2.2:65391653-65391672 | None:intergenic | 55.0% | |
| !! | AACGGTTTTAGCAGCGGTGG+TGG | + | chr2.2:65391773-65391792 | None:intergenic | 55.0% |
| CACCGGTGCAGTGATAGCAG+TGG | - | chr2.2:65391582-65391601 | MS.gene96245:CDS | 60.0% | |
| !! | GGTTTTAGCAGCGGTGGTGG+AGG | + | chr2.2:65391770-65391789 | None:intergenic | 60.0% |
| GCGTTAGGGGCGGGGTTGAG+TGG | + | chr2.2:65391739-65391758 | None:intergenic | 70.0% | |
| !! | AGCAGCGGTGGTGGAGGCGA+CGG | + | chr2.2:65391764-65391783 | None:intergenic | 70.0% |
| !! | TGGAGGCGACGGGGGCGTTA+GGG | + | chr2.2:65391753-65391772 | None:intergenic | 70.0% |
| !! | AGCGGTGGTGGAGGCGACGG+GGG | + | chr2.2:65391761-65391780 | None:intergenic | 75.0% |
| !! | CAGCGGTGGTGGAGGCGACG+GGG | + | chr2.2:65391762-65391781 | None:intergenic | 75.0% |
| !! | GCAGCGGTGGTGGAGGCGAC+GGG | + | chr2.2:65391763-65391782 | None:intergenic | 75.0% |
| !! | GGAGGCGACGGGGGCGTTAG+GGG | + | chr2.2:65391752-65391771 | None:intergenic | 75.0% |
| !! | GTGGAGGCGACGGGGGCGTT+AGG | + | chr2.2:65391754-65391773 | None:intergenic | 75.0% |
| !!! | CGACGGGGGCGTTAGGGGCG+GGG | + | chr2.2:65391747-65391766 | None:intergenic | 80.0% |
| !!! | GCGACGGGGGCGTTAGGGGC+GGG | + | chr2.2:65391748-65391767 | None:intergenic | 80.0% |
| !!! | GGCGACGGGGGCGTTAGGGG+CGG | + | chr2.2:65391749-65391768 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.2 | gene | 65391571 | 65391798 | 65391571 | ID=MS.gene96245 |
| chr2.2 | mRNA | 65391571 | 65391798 | 65391571 | ID=MS.gene96245.t1;Parent=MS.gene96245 |
| chr2.2 | exon | 65391571 | 65391798 | 65391571 | ID=MS.gene96245.t1.exon1;Parent=MS.gene96245.t1 |
| chr2.2 | CDS | 65391571 | 65391798 | 65391571 | ID=cds.MS.gene96245.t1;Parent=MS.gene96245.t1 |
| Gene Sequence |
| Protein sequence |
