Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96344.t1 | XP_003610067.2 | 96.4 | 165 | 6 | 0 | 1 | 165 | 1 | 165 | 2.80E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96344.t1 | Q93Z27 | 42.2 | 166 | 78 | 3 | 6 | 164 | 16 | 170 | 1.1e-21 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96344.t1 | G7JUB9 | 96.4 | 165 | 6 | 0 | 1 | 165 | 1 | 165 | 2.0e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04998 | MS.gene96344 | 0.818724 | 1.69E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene96344 | 0.812876 | 3.40E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene96344 | 0.804918 | 1.71E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene96344 | 0.85474 | 1.01E-61 | -1.69E-46 |
| MS.gene053298 | MS.gene96344 | 0.805959 | 1.04E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene96344 | 0.802402 | 5.71E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene96344 | 0.818434 | 1.96E-52 | -1.69E-46 |
| MS.gene054576 | MS.gene96344 | 0.806753 | 7.06E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene96344 | 0.871549 | 6.28E-67 | -1.69E-46 |
| MS.gene054893 | MS.gene96344 | 0.869193 | 3.72E-66 | -1.69E-46 |
| MS.gene055226 | MS.gene96344 | 0.813877 | 2.05E-51 | -1.69E-46 |
| MS.gene056315 | MS.gene96344 | 0.805359 | 1.39E-49 | -1.69E-46 |
| MS.gene056699 | MS.gene96344 | 0.812949 | 3.28E-51 | -1.69E-46 |
| MS.gene057155 | MS.gene96344 | 0.800762 | 1.24E-48 | -1.69E-46 |
| MS.gene058526 | MS.gene96344 | 0.812151 | 4.90E-51 | -1.69E-46 |
| MS.gene058527 | MS.gene96344 | 0.814539 | 1.46E-51 | -1.69E-46 |
| MS.gene058528 | MS.gene96344 | 0.814097 | 1.83E-51 | -1.69E-46 |
| MS.gene058592 | MS.gene96344 | 0.822079 | 2.87E-53 | -1.69E-46 |
| MS.gene05952 | MS.gene96344 | 0.82147 | 3.97E-53 | -1.69E-46 |
| MS.gene059774 | MS.gene96344 | 0.835832 | 1.33E-56 | -1.69E-46 |
| MS.gene05985 | MS.gene96344 | -0.815972 | 7.03E-52 | -1.69E-46 |
| MS.gene059965 | MS.gene96344 | 0.804201 | 2.42E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene96344 | 0.810461 | 1.14E-50 | -1.69E-46 |
| MS.gene060412 | MS.gene96344 | 0.826557 | 2.54E-54 | -1.69E-46 |
| MS.gene060697 | MS.gene96344 | 0.832561 | 8.81E-56 | -1.69E-46 |
| MS.gene060840 | MS.gene96344 | 0.802476 | 5.51E-49 | -1.69E-46 |
| MS.gene061047 | MS.gene96344 | 0.866156 | 3.50E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96344.t1 | MTR_4g127560 | 96.825 | 189 | 6 | 0 | 1 | 189 | 1 | 189 | 2.02e-131 | 367 |
| MS.gene96344.t1 | MTR_5g025690 | 64.286 | 182 | 57 | 2 | 8 | 189 | 18 | 191 | 1.69e-74 | 223 |
| MS.gene96344.t1 | MTR_7g011010 | 62.366 | 186 | 66 | 3 | 6 | 189 | 10 | 193 | 1.22e-65 | 200 |
| MS.gene96344.t1 | MTR_4g082050 | 48.182 | 110 | 52 | 4 | 81 | 189 | 29 | 134 | 5.11e-21 | 84.7 |
| MS.gene96344.t1 | MTR_7g074240 | 48.193 | 83 | 42 | 1 | 108 | 189 | 84 | 166 | 2.15e-15 | 70.9 |
| MS.gene96344.t1 | MTR_2g098890 | 29.474 | 190 | 113 | 4 | 19 | 189 | 11 | 198 | 1.98e-14 | 68.9 |
| MS.gene96344.t1 | MTR_2g023310 | 61.111 | 54 | 21 | 0 | 78 | 131 | 44 | 97 | 1.99e-14 | 66.2 |
| MS.gene96344.t1 | MTR_4g067270 | 49.180 | 61 | 31 | 0 | 129 | 189 | 77 | 137 | 1.36e-12 | 62.8 |
| MS.gene96344.t1 | MTR_3g109320 | 47.619 | 63 | 33 | 0 | 129 | 191 | 114 | 176 | 1.77e-12 | 63.2 |
| MS.gene96344.t1 | MTR_7g089760 | 51.786 | 56 | 27 | 0 | 134 | 189 | 82 | 137 | 4.00e-11 | 58.9 |
| MS.gene96344.t1 | MTR_3g088655 | 40.659 | 91 | 49 | 1 | 99 | 189 | 65 | 150 | 9.52e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96344.t1 | AT5G39670 | 47.644 | 191 | 87 | 6 | 2 | 189 | 15 | 195 | 5.29e-43 | 143 |
| MS.gene96344.t1 | AT3G29000 | 61.165 | 103 | 38 | 1 | 87 | 189 | 85 | 185 | 2.62e-35 | 123 |
| MS.gene96344.t1 | AT4G20780 | 61.667 | 60 | 23 | 0 | 130 | 189 | 120 | 179 | 9.78e-18 | 77.4 |
| MS.gene96344.t1 | AT5G44460 | 56.667 | 60 | 26 | 0 | 130 | 189 | 111 | 170 | 3.17e-15 | 70.9 |
| MS.gene96344.t1 | AT3G47480 | 50.704 | 71 | 33 | 2 | 120 | 189 | 105 | 174 | 3.81e-15 | 70.5 |
| MS.gene96344.t1 | AT3G59440 | 29.891 | 184 | 108 | 6 | 19 | 189 | 7 | 182 | 5.78e-15 | 70.1 |
| MS.gene96344.t1 | AT2G43290 | 37.615 | 109 | 64 | 3 | 83 | 189 | 96 | 202 | 1.87e-14 | 69.3 |
| MS.gene96344.t1 | AT4G03290 | 54.237 | 59 | 27 | 0 | 131 | 189 | 82 | 140 | 2.55e-14 | 67.8 |
| MS.gene96344.t1 | AT1G05990 | 52.542 | 59 | 28 | 0 | 131 | 189 | 80 | 138 | 5.20e-14 | 66.6 |
| MS.gene96344.t1 | AT3G07490 | 53.571 | 56 | 26 | 0 | 134 | 189 | 82 | 137 | 3.09e-13 | 64.7 |
| MS.gene96344.t1 | AT4G12860 | 53.571 | 56 | 26 | 0 | 134 | 189 | 82 | 137 | 3.30e-12 | 62.0 |
| MS.gene96344.t1 | AT1G21550 | 31.496 | 127 | 63 | 3 | 84 | 189 | 25 | 148 | 1.67e-11 | 60.1 |
Find 22 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGAGAGTTCTTGTTATTT+TGG | 0.121543 | 8.4:+6928101 | MS.gene96344:CDS |
| AACTCAACTTCTCCCTTATT+TGG | 0.181162 | 8.4:+6927688 | MS.gene96344:CDS |
| CTTCTTTGCCAAATTCATTC+TGG | 0.280945 | 8.4:+6927784 | MS.gene96344:CDS |
| CTGAGCTACCAGAATGAATT+TGG | 0.286669 | 8.4:-6927792 | None:intergenic |
| TGAAGAGAATGAGCCTAGTT+TGG | 0.350779 | 8.4:+6928011 | MS.gene96344:CDS |
| GCAAATGTTGGCAGAGATAA+TGG | 0.359399 | 8.4:+6927871 | MS.gene96344:CDS |
| TGAAAGAGATGAAGTGAAAA+TGG | 0.369654 | 8.4:+6927903 | MS.gene96344:CDS |
| AAGGTCTATGAAACCAAATA+AGG | 0.374441 | 8.4:-6927701 | None:intergenic |
| CAGAATGAATTTGGCAAAGA+AGG | 0.401758 | 8.4:-6927783 | None:intergenic |
| GAGGAACTTGAAGAAAAGTA+TGG | 0.414511 | 8.4:+6927964 | MS.gene96344:CDS |
| CTATTACTATGTGAAATTTG+AGG | 0.460627 | 8.4:-6927658 | None:intergenic |
| AAACACTTCACAAAGCTCCT+TGG | 0.468222 | 8.4:-6927989 | None:intergenic |
| AGGTCTATGAAACCAAATAA+GGG | 0.516554 | 8.4:-6927700 | None:intergenic |
| TGCTTCAACTCCTCCAAACT+AGG | 0.518183 | 8.4:-6928024 | None:intergenic |
| AGAGGTAAGAGATGAGAAGA+AGG | 0.519907 | 8.4:+6927813 | MS.gene96344:CDS |
| AGAGAATGAGCCTAGTTTGG+AGG | 0.522617 | 8.4:+6928014 | MS.gene96344:CDS |
| AAAATGTCAAGTATAGGAAA+AGG | 0.526434 | 8.4:-6927720 | None:intergenic |
| GTGTAGCAGTCAAGAGAATG+AGG | 0.533790 | 8.4:+6927849 | MS.gene96344:CDS |
| CAAGAGAATGAGGCAAATGT+TGG | 0.535719 | 8.4:+6927859 | MS.gene96344:CDS |
| TGAAGAAAAGTATGGTTCCA+AGG | 0.559713 | 8.4:+6927972 | MS.gene96344:CDS |
| AGATGAAGTGAAAATGGTGA+TGG | 0.595216 | 8.4:+6927909 | MS.gene96344:CDS |
| AATTCATTCTGGTAGCTCAG+AGG | 0.688478 | 8.4:+6927795 | MS.gene96344:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAGATTTAAATGTGTAAT+TGG | + | chr8.4:6928219-6928238 | MS.gene96344:intron | 15.0% |
| !! | CTTTGTAAAAATATTGATAT+TGG | - | chr8.4:6928484-6928503 | None:intergenic | 15.0% |
| !! | ATACGAATATCCTTAAATAA+GGG | - | chr8.4:6928699-6928718 | None:intergenic | 20.0% |
| !! | ATATACTTGAGAAAAAATTG+TGG | - | chr8.4:6927756-6927775 | None:intergenic | 20.0% |
| !! | CTTTGTAAAAATATTGACAT+TGG | + | chr8.4:6928310-6928329 | MS.gene96344:intron | 20.0% |
| !! | CTTTGTAAAAATATTGTCAT+TGG | - | chr8.4:6928544-6928563 | None:intergenic | 20.0% |
| !! | TATACGAATATCCTTAAATA+AGG | - | chr8.4:6928700-6928719 | None:intergenic | 20.0% |
| !!! | ACAATTTTTTCTCAAGTATA+TGG | + | chr8.4:6927755-6927774 | MS.gene96344:CDS | 20.0% |
| !!! | ATTTTTGATGAAAATCAAGA+TGG | + | chr8.4:6928171-6928190 | MS.gene96344:CDS | 20.0% |
| !!! | GGTTTTTCATAATATTTACA+AGG | - | chr8.4:6928652-6928671 | None:intergenic | 20.0% |
| ! | AAAATGTCAAGTATAGGAAA+AGG | - | chr8.4:6927723-6927742 | None:intergenic | 25.0% |
| ! | ACAAGGTTTAAATGTGTAAT+TGG | - | chr8.4:6928635-6928654 | None:intergenic | 25.0% |
| ! | CTATTACTATGTGAAATTTG+AGG | - | chr8.4:6927661-6927680 | None:intergenic | 25.0% |
| ! | CTTTGAACAAATATTGGTAT+TGG | + | chr8.4:6928401-6928420 | MS.gene96344:intron | 25.0% |
| ! | TGTTGAAAAATGTCAAGTAT+AGG | - | chr8.4:6927729-6927748 | None:intergenic | 25.0% |
| !! | CAAAAATTGTGATCATCTTT+TGG | - | chr8.4:6928158-6928177 | None:intergenic | 25.0% |
| !! | CAAAATTTTTACAAAGTGCA+GGG | + | chr8.4:6928457-6928476 | MS.gene96344:intron | 25.0% |
| !!! | AAAATGGTGATGGAAAAAAT+GGG | + | chr8.4:6927919-6927938 | MS.gene96344:CDS | 25.0% |
| !!! | ACAATATTTTTACAAAGTGC+AGG | + | chr8.4:6928547-6928566 | MS.gene96344:intron | 25.0% |
| !!! | CAATATTTTTACAAAGTGCA+AGG | + | chr8.4:6928488-6928507 | MS.gene96344:intron | 25.0% |
| !!! | CAATATTTTTACAAAGTGCA+GGG | + | chr8.4:6928488-6928507 | MS.gene96344:intron | 25.0% |
| !!! | CAATATTTTTACAAAGTGCA+GGG | - | chr8.4:6928507-6928488 | None:intergenic | 25.0% |
| !!! | CAATTTTTTTACAAACTGCA+GGG | - | chr8.4:6928366-6928385 | None:intergenic | 25.0% |
| !!! | CACTTTTTTTTGTTTGACAT+TGG | - | chr8.4:6928515-6928534 | None:intergenic | 25.0% |
| !!! | TCAATATTTTTACAAAGTGC+AGG | - | chr8.4:6928506-6928487 | None:intergenic | 25.0% |
| !!! | TTTTCAACAGATTTTGACAT+TGG | + | chr8.4:6928250-6928269 | MS.gene96344:intron | 25.0% |
| AAGGTCTATGAAACCAAATA+AGG | - | chr8.4:6927704-6927723 | None:intergenic | 30.0% | |
| AGGTCTATGAAACCAAATAA+GGG | - | chr8.4:6927703-6927722 | None:intergenic | 30.0% | |
| CAAAATCTGTTGAAAATGCA+GGG | - | chr8.4:6928246-6928265 | None:intergenic | 30.0% | |
| CAATATTTGTTCAAAGTGCA+GGG | - | chr8.4:6928397-6928416 | None:intergenic | 30.0% | |
| CCTGCAGTTTGTAAAAAAAT+TGG | + | chr8.4:6928364-6928383 | MS.gene96344:intron | 30.0% | |
| TCAAAATCTGTTGAAAATGC+AGG | - | chr8.4:6928247-6928266 | None:intergenic | 30.0% | |
| TGAAAGAGATGAAGTGAAAA+TGG | + | chr8.4:6927903-6927922 | MS.gene96344:CDS | 30.0% | |
| ! | CCAAAATTTTTACAAAGTGC+AGG | + | chr8.4:6928456-6928475 | MS.gene96344:intron | 30.0% |
| ! | CCTGCACTTTGTAAAAATTT+TGG | - | chr8.4:6928459-6928478 | None:intergenic | 30.0% |
| ! | GTCAAACAAAAAAAAGTGCA+GGG | + | chr8.4:6928517-6928536 | MS.gene96344:intron | 30.0% |
| ! | GTTTTTGATGAGAACAAAGA+TGG | + | chr8.4:6928057-6928076 | MS.gene96344:CDS | 30.0% |
| ! | TGTCAAACAAAAAAAAGTGC+AGG | + | chr8.4:6928516-6928535 | MS.gene96344:intron | 30.0% |
| !! | GAAAATGGTGATGGAAAAAA+TGG | + | chr8.4:6927918-6927937 | MS.gene96344:CDS | 30.0% |
| !! | GTTTGTTGTTCCCTTATTTA+AGG | + | chr8.4:6928686-6928705 | MS.gene96344:intron | 30.0% |
| !!! | AGATGGTTTTATTGATGCAA+AGG | + | chr8.4:6928074-6928093 | MS.gene96344:CDS | 30.0% |
| !!! | CACTTTTTTTTGTTTGGCAT+TGG | + | chr8.4:6928279-6928298 | MS.gene96344:intron | 30.0% |
| !!! | CACTTTTTTTTGTTTGGCAT+TGG | - | chr8.4:6928298-6928279 | None:intergenic | 30.0% |
| !!! | CAGAGAGTTCTTGTTATTTT+GGG | + | chr8.4:6928102-6928121 | MS.gene96344:CDS | 30.0% |
| !!! | CCAATTTTTTTACAAACTGC+AGG | - | chr8.4:6928367-6928386 | None:intergenic | 30.0% |
| !!! | GTTATTTTGGGATTGAAACA+AGG | + | chr8.4:6928114-6928133 | MS.gene96344:CDS | 30.0% |
| AACAAACAAATCAGCAGAAG+TGG | - | chr8.4:6928673-6928692 | None:intergenic | 35.0% | |
| AACTCAACTTCTCCCTTATT+TGG | + | chr8.4:6927688-6927707 | MS.gene96344:CDS | 35.0% | |
| AGATGAAGTGAAAATGGTGA+TGG | + | chr8.4:6927909-6927928 | MS.gene96344:CDS | 35.0% | |
| CAAAATCTGTTGAAACTGCA+AGG | + | chr8.4:6928608-6928627 | MS.gene96344:intron | 35.0% | |
| CAGAATGAATTTGGCAAAGA+AGG | - | chr8.4:6927786-6927805 | None:intergenic | 35.0% | |
| CCAATATTTGTTCAAAGTGC+AGG | - | chr8.4:6928398-6928417 | None:intergenic | 35.0% | |
| CCTGCACTTTGAACAAATAT+TGG | + | chr8.4:6928395-6928414 | MS.gene96344:intron | 35.0% | |
| CTTCTTTGCCAAATTCATTC+TGG | + | chr8.4:6927784-6927803 | MS.gene96344:CDS | 35.0% | |
| GAGGAACTTGAAGAAAAGTA+TGG | + | chr8.4:6927964-6927983 | MS.gene96344:CDS | 35.0% | |
| ! | CTTGCAGTTTCAACAGATTT+TGG | - | chr8.4:6928610-6928629 | None:intergenic | 35.0% |
| ! | GCCAAACAAAAAAAAGTGCA+GGG | + | chr8.4:6928577-6928596 | MS.gene96344:intron | 35.0% |
| ! | GCCAAACAAAAAAAAGTGCA+GGG | - | chr8.4:6928596-6928577 | None:intergenic | 35.0% |
| ! | TGAAGAAAAGTATGGTTCCA+AGG | + | chr8.4:6927972-6927991 | MS.gene96344:CDS | 35.0% |
| ! | TGCCAAACAAAAAAAAGTGC+AGG | + | chr8.4:6928576-6928595 | MS.gene96344:intron | 35.0% |
| ! | TGCCAAACAAAAAAAAGTGC+AGG | - | chr8.4:6928595-6928576 | None:intergenic | 35.0% |
| !! | GTTTCAACAGATTTTGGCAT+TGG | - | chr8.4:6928604-6928623 | None:intergenic | 35.0% |
| !!! | GCAGAGAGTTCTTGTTATTT+TGG | + | chr8.4:6928101-6928120 | MS.gene96344:CDS | 35.0% |
| !!! | TCCCTGCACTTTTTTTTGTT+TGG | + | chr8.4:6928273-6928292 | MS.gene96344:intron | 35.0% |
| !!! | TCCCTGCACTTTTTTTTGTT+TGG | - | chr8.4:6928292-6928273 | None:intergenic | 35.0% |
| AAACACTTCACAAAGCTCCT+TGG | - | chr8.4:6927992-6928011 | None:intergenic | 40.0% | |
| AATTCATTCTGGTAGCTCAG+AGG | + | chr8.4:6927795-6927814 | MS.gene96344:CDS | 40.0% | |
| AGAGGTAAGAGATGAGAAGA+AGG | + | chr8.4:6927813-6927832 | MS.gene96344:CDS | 40.0% | |
| CAAGAGAATGAGGCAAATGT+TGG | + | chr8.4:6927859-6927878 | MS.gene96344:CDS | 40.0% | |
| CTGAGCTACCAGAATGAATT+TGG | - | chr8.4:6927795-6927814 | None:intergenic | 40.0% | |
| GCAAATGTTGGCAGAGATAA+TGG | + | chr8.4:6927871-6927890 | MS.gene96344:CDS | 40.0% | |
| TGAAGAGAATGAGCCTAGTT+TGG | + | chr8.4:6928011-6928030 | MS.gene96344:CDS | 40.0% | |
| AGAGAATGAGCCTAGTTTGG+AGG | + | chr8.4:6928014-6928033 | MS.gene96344:CDS | 45.0% | |
| CCACGTGGCATGAAATGATT+GGG | + | chr8.4:6928426-6928445 | MS.gene96344:intron | 45.0% | |
| GTGTAGCAGTCAAGAGAATG+AGG | + | chr8.4:6927849-6927868 | MS.gene96344:CDS | 45.0% | |
| TGCTTCAACTCCTCCAAACT+AGG | - | chr8.4:6928027-6928046 | None:intergenic | 45.0% | |
| ! | CTTTTGCAGCTCAGAAAGTG+AGG | + | chr8.4:6927945-6927964 | MS.gene96344:CDS | 45.0% |
| !! | ATATTGGTATTGGTGCCACG+TGG | + | chr8.4:6928411-6928430 | MS.gene96344:intron | 45.0% |
| CCCAATCATTTCATGCCACG+TGG | - | chr8.4:6928429-6928448 | None:intergenic | 50.0% | |
| GCCACGTGGCATGAAATGAT+TGG | + | chr8.4:6928425-6928444 | MS.gene96344:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6927649 | 6928725 | 6927649 | ID=MS.gene96344 |
| chr8.4 | mRNA | 6927649 | 6928725 | 6927649 | ID=MS.gene96344.t1;Parent=MS.gene96344 |
| chr8.4 | exon | 6927649 | 6928215 | 6927649 | ID=MS.gene96344.t1.exon1;Parent=MS.gene96344.t1 |
| chr8.4 | CDS | 6927649 | 6928215 | 6927649 | ID=cds.MS.gene96344.t1;Parent=MS.gene96344.t1 |
| chr8.4 | exon | 6928708 | 6928725 | 6928708 | ID=MS.gene96344.t1.exon2;Parent=MS.gene96344.t1 |
| chr8.4 | CDS | 6928708 | 6928725 | 6928708 | ID=cds.MS.gene96344.t1;Parent=MS.gene96344.t1 |
| Gene Sequence |
| Protein sequence |
