AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048072.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048072.01.T01 MTR_4g085900 84.167 240 11 1 62 274 205 444 2.11e-134 389
MsG0080048072.01.T01 MTR_4g085900 45.000 180 96 3 99 276 65 243 1.14e-34 130
MsG0080048072.01.T01 MTR_4g085900 96.774 62 2 0 1 62 76 137 1.29e-31 122
MsG0080048072.01.T01 MTR_4g085900 53.623 69 32 0 1 69 280 348 4.66e-12 66.6
MsG0080048072.01.T01 MTR_4g085900 77.099 131 3 1 62 165 205 335 1.77e-57 188
MsG0080048072.01.T01 MTR_4g085900 45.000 180 96 3 99 276 65 243 9.36e-36 131
MsG0080048072.01.T01 MTR_4g085900 93.846 65 4 0 1 65 76 140 1.96e-32 122
MsG0080048072.01.T01 MTR_2g078060 45.532 235 100 3 67 273 200 434 1.37e-53 181
MsG0080048072.01.T01 MTR_2g078060 41.111 180 104 2 99 276 54 233 5.30e-32 123
MsG0080048072.01.T01 MTR_2g078060 57.353 68 29 0 1 68 65 132 5.65e-15 75.1
MsG0080048072.01.T01 MTR_2g078060 46.377 69 37 0 1 69 270 338 8.68e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048072.01.T01 AT4G34020 55.000 240 81 2 62 274 72 311 2.97e-83 253
MsG0080048072.01.T01 AT4G34020 37.963 108 66 1 170 276 3 110 3.78e-14 72.0
MsG0080048072.01.T01 AT4G34020 55.000 240 81 2 62 274 72 311 2.97e-83 253
MsG0080048072.01.T01 AT4G34020 37.963 108 66 1 170 276 3 110 3.78e-14 72.0
MsG0080048072.01.T01 AT4G34020 55.000 240 81 2 62 274 191 430 1.41e-81 253
MsG0080048072.01.T01 AT4G34020 39.597 149 89 1 129 276 81 229 3.26e-25 104
MsG0080048072.01.T01 AT4G34020 55.000 240 81 2 62 274 226 465 2.85e-81 253
MsG0080048072.01.T01 AT4G34020 44.693 179 98 1 99 276 86 264 9.34e-41 147
MsG0080048072.01.T01 AT4G34020 64.516 62 22 0 1 62 97 158 1.20e-19 89.0
MsG0080048072.01.T01 AT1G53280 42.194 237 109 2 65 273 199 435 1.57e-51 175
MsG0080048072.01.T01 AT1G53280 45.000 180 97 2 99 276 55 234 5.44e-43 153
MsG0080048072.01.T01 AT1G53280 55.072 69 31 0 1 69 66 134 1.87e-15 76.6
MsG0080048072.01.T01 AT3G14990 43.038 237 107 2 65 273 130 366 7.74e-48 164
MsG0080048072.01.T01 AT3G14990 42.424 165 92 2 115 276 1 165 3.00e-35 130
MsG0080048072.01.T01 AT3G14990 54.688 64 29 0 6 69 1 64 1.68e-13 70.5
MsG0080048072.01.T01 AT3G14990 43.038 237 107 2 65 273 130 366 7.74e-48 164
MsG0080048072.01.T01 AT3G14990 42.424 165 92 2 115 276 1 165 3.00e-35 130
MsG0080048072.01.T01 AT3G14990 54.688 64 29 0 6 69 1 64 1.68e-13 70.5
MsG0080048072.01.T01 AT3G14990 43.038 237 107 2 65 273 153 389 1.04e-47 164
MsG0080048072.01.T01 AT3G14990 44.751 181 97 2 99 276 8 188 1.27e-43 153
MsG0080048072.01.T01 AT3G14990 55.072 69 31 0 1 69 19 87 3.65e-15 75.5
MsG0080048072.01.T01 AT1G53280 45.000 180 97 2 99 276 55 234 1.53e-43 153
MsG0080048072.01.T01 AT1G53280 42.857 175 72 2 65 211 199 373 2.29e-36 134
MsG0080048072.01.T01 AT1G53280 55.072 69 31 0 1 69 66 134 1.51e-15 76.6

Find 82 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTTATCAGCAACAATTT+TGG 0.218461 contig192end:-2271 None:intergenic
CCTTGGCTGAGCAGCTGTTT+GGG 0.241099 contig192end:+1009 MsG0080048072.01.T01:CDS
GCTATTGTGAGCAAGTTATT+TGG 0.265797 contig192end:+3281 MsG0080048072.01.T01:CDS
ACCTTGGCTGAGCAGCTGTT+TGG 0.305489 contig192end:+1008 MsG0080048072.01.T01:CDS
AAGGGAGGTTGCTGAATTCT+TGG 0.306017 contig192end:+1043 MsG0080048072.01.T01:CDS
TTACATGTTTGCTTTAACCT+TGG 0.343551 contig192end:+992 MsG0080048072.01.T01:CDS
TAGGTGTGATAGAAATTTAT+AGG 0.369080 contig192end:+3391 MsG0080048072.01.T01:CDS
AATTCAGCTGGAAGAATATA+CGG 0.369869 contig192end:+2995 MsG0080048072.01.T01:CDS
TTTAAGATTGTCCATGGAAG+AGG 0.381844 contig192end:-3171 None:intergenic
TAATCAAAACAATGCGTTAA+GGG 0.386916 contig192end:-3468 None:intergenic
TGAGAAAACTCTTGGAGTCT+TGG 0.397662 contig192end:+2236 MsG0080048072.01.T01:CDS
AAAACAAATCAGGCTTGGGC+TGG 0.402388 contig192end:-3423 None:intergenic
ATGTGGTTTCAAGTACTTGT+AGG 0.413572 contig192end:+3372 MsG0080048072.01.T01:CDS
GCTTTACCTGGAGGAATGCC+TGG 0.415338 contig192end:+521 MsG0080048072.01.T01:CDS
CAGCTTCAAGTTGTAGCTGC+TGG 0.418300 contig192end:+434 MsG0080048072.01.T01:CDS
CTAATCAAAACAATGCGTTA+AGG 0.420856 contig192end:-3469 None:intergenic
ACACCACCTGAAAACAAATC+AGG 0.421102 contig192end:-3433 None:intergenic
TCTTCAGAACCATGCGCGAT+TGG 0.430665 contig192end:-2147 None:intergenic
TGACACTGATAGATATTCTA+AGG 0.431244 contig192end:+2181 MsG0080048072.01.T01:CDS
GCTTCTGTTGAGAAAACTCT+TGG 0.435054 contig192end:+2228 MsG0080048072.01.T01:CDS
ACCTGAAAACAAATCAGGCT+TGG 0.437458 contig192end:-3428 None:intergenic
GTCCAACATTCAGGTTCAAA+CGG 0.437586 contig192end:+939 MsG0080048072.01.T01:intron
GCAAACATGTAAGCAGTTCC+CGG 0.440613 contig192end:-981 None:intergenic
GTGAGCAAGTTATTTGGTAA+TGG 0.442489 contig192end:+3287 MsG0080048072.01.T01:CDS
CTTCAGAACCATGCGCGATT+GGG 0.443538 contig192end:-2146 None:intergenic
ACATGATTTGATTATTCTCC+CGG 0.445948 contig192end:+2317 MsG0080048072.01.T01:CDS
GCTTACAACAAGCCGTGGCC+CGG 0.454304 contig192end:+962 MsG0080048072.01.T01:CDS
CTCAAAGAACAAAATTCAGC+TGG 0.460880 contig192end:+2983 MsG0080048072.01.T01:CDS
AAACAAATCAGGCTTGGGCT+GGG 0.463419 contig192end:-3422 None:intergenic
ATCCACGTCCTCCGTCGTGC+TGG 0.464137 contig192end:+380 MsG0080048072.01.T01:CDS
CACCAGCACGACGGAGGACG+TGG 0.465570 contig192end:-382 None:intergenic
CCTTGTTTGTGTAGAATAGC+AGG 0.466355 contig192end:-3034 None:intergenic
TTTGATCTTATTGCTTTACC+TGG 0.471486 contig192end:+509 MsG0080048072.01.T01:CDS
AAATTTCAGGGAGGAACTGC+TGG 0.472046 contig192end:+2920 MsG0080048072.01.T01:intron
CACTGTAACGGAGTTTGCAT+TGG 0.475926 contig192end:+3259 MsG0080048072.01.T01:CDS
CTGCAGAGTTGAAAATATGC+TGG 0.476502 contig192end:+3637 MsG0080048072.01.T01:three_prime_UTR
CTTGGCTGAGCAGCTGTTTG+GGG 0.476503 contig192end:+1010 MsG0080048072.01.T01:CDS
TCCAACATTCAGGTTCAAAC+GGG 0.484243 contig192end:+940 MsG0080048072.01.T01:intron
GATTCAGAGGACATGGTGAT+TGG 0.485961 contig192end:-3502 None:intergenic
CCTGAAAACAAATCAGGCTT+GGG 0.487349 contig192end:-3427 None:intergenic
GATGCCGTAGTAGTTATTGA+TGG 0.498779 contig192end:+3209 MsG0080048072.01.T01:CDS
TGTAAGCAGTTCCCGGGCCA+CGG 0.500404 contig192end:-974 None:intergenic
TGGGGAGTCTGTTGCAAGGG+AGG 0.503381 contig192end:+1028 MsG0080048072.01.T01:CDS
CTTGGAGTCTTGGCTTCTCA+AGG 0.507724 contig192end:+2246 MsG0080048072.01.T01:CDS
ACACAAACAAGGTTTACTAA+AGG 0.507836 contig192end:+3045 MsG0080048072.01.T01:CDS
GCTCTAGTAGTCTATTTCCT+AGG 0.517668 contig192end:-3349 None:intergenic
GTTTCAGCTTAGTTGAACAT+TGG 0.518754 contig192end:+3598 MsG0080048072.01.T01:three_prime_UTR
AACAAATCAGGCTTGGGCTG+GGG 0.522982 contig192end:-3421 None:intergenic
AATAGCAGGTGAGGAGCAGA+CGG 0.534057 contig192end:-3020 None:intergenic
CTTACAACAAGCCGTGGCCC+GGG 0.537057 contig192end:+963 MsG0080048072.01.T01:CDS
GTTTGGGGAGTCTGTTGCAA+GGG 0.539143 contig192end:+1025 MsG0080048072.01.T01:CDS
CTGAGGCTCAACGGAAGCTA+CGG 0.539984 contig192end:-414 None:intergenic
GCTGAACCAGGCATTCCTCC+AGG 0.543196 contig192end:-527 None:intergenic
CCCAAACAGCTGCTCAGCCA+AGG 0.560232 contig192end:-1009 None:intergenic
GAGGAACTGCTGGCGCTCAA+AGG 0.560324 contig192end:+2930 MsG0080048072.01.T01:CDS
AATCAAAACAATGCGTTAAG+GGG 0.564100 contig192end:-3467 None:intergenic
TGCAGAGTTGAAAATATGCT+GGG 0.567483 contig192end:+3638 MsG0080048072.01.T01:three_prime_UTR
GCATAGGAAAAGTTACCTGC+AGG 0.567675 contig192end:+3545 MsG0080048072.01.T01:three_prime_UTR
TGTTTGGGGAGTCTGTTGCA+AGG 0.568984 contig192end:+1024 MsG0080048072.01.T01:CDS
AACTTGAAGCTGAGGCTCAA+CGG 0.571880 contig192end:-423 None:intergenic
CCTGCTATTCTACACAAACA+AGG 0.583795 contig192end:+3034 MsG0080048072.01.T01:CDS
TTTGCCATCAATAACTACTA+CGG 0.585134 contig192end:-3213 None:intergenic
TTAAGATTGTCCATGGAAGA+GGG 0.586260 contig192end:-3170 None:intergenic
TGAGGGACTTGCCACTGTAA+CGG 0.595366 contig192end:+3247 MsG0080048072.01.T01:CDS
CAAACATGTAAGCAGTTCCC+GGG 0.595831 contig192end:-980 None:intergenic
GTTCTCATCCCAATCGCGCA+TGG 0.596389 contig192end:+2138 MsG0080048072.01.T01:CDS
TACAGCTCATCCCTCTTCCA+TGG 0.598748 contig192end:+3160 MsG0080048072.01.T01:CDS
GATCTTATTGCTTTACCTGG+AGG 0.601166 contig192end:+512 MsG0080048072.01.T01:CDS
TCCGTCTTTAAGATTGTCCA+TGG 0.601178 contig192end:-3177 None:intergenic
AAAATGAGATTCAGAGGACA+TGG 0.603668 contig192end:-3509 None:intergenic
CTTCAAGTTGTAGCTGCTGG+AGG 0.607280 contig192end:+437 MsG0080048072.01.T01:CDS
TCCATGGACAATCTTAAAGA+CGG 0.618927 contig192end:+3176 MsG0080048072.01.T01:CDS
GCAGCTACAACTTGAAGCTG+AGG 0.621580 contig192end:-431 None:intergenic
ATCAAAACAATGCGTTAAGG+GGG 0.622614 contig192end:-3466 None:intergenic
AACGGGCTTACAACAAGCCG+TGG 0.632694 contig192end:+957 MsG0080048072.01.T01:CDS
AAATAGACTACTAGAGCATG+TGG 0.634408 contig192end:+3355 MsG0080048072.01.T01:CDS
GGCAAAGTGATCACTTGTGA+GGG 0.639904 contig192end:+3230 MsG0080048072.01.T01:CDS
TTTGTGTAGAATAGCAGGTG+AGG 0.652165 contig192end:-3029 None:intergenic
CAATGCAAACTCCGTTACAG+TGG 0.660685 contig192end:-3258 None:intergenic
CATGAGCACCAGCACGACGG+AGG 0.694711 contig192end:-388 None:intergenic
TGGCAAAGTGATCACTTGTG+AGG 0.698032 contig192end:+3229 MsG0080048072.01.T01:CDS
TGACATGAGCACCAGCACGA+CGG 0.727381 contig192end:-391 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGTTTGTTTATTATTT+TGG + contig192end:825-844 MsG0080048072.01.T01:intron 10.0%
!! ACCTATAAACTTATATTTAT+GGG + contig192end:2812-2831 MsG0080048072.01.T01:intron 15.0%
!! ATTTCAAAAAACAATGAAAA+CGG + contig192end:1524-1543 MsG0080048072.01.T01:intron 15.0%
!! TAAATAATATAGAGCATATA+TGG + contig192end:2646-2665 MsG0080048072.01.T01:intron 15.0%
!!! GCTCTATATTATTTATTTTA+AGG - contig192end:2641-2660 None:intergenic 15.0%
!!! TTGTTTATTATTTTGGTATT+TGG + contig192end:832-851 MsG0080048072.01.T01:intron 15.0%
!! AAACGTTATTAAAAGTAAAC+AGG - contig192end:1397-1416 None:intergenic 20.0%
!! ATCTTCAATGTTCAAAATTT+AGG + contig192end:1669-1688 MsG0080048072.01.T01:intron 20.0%
!! CACCTATAAACTTATATTTA+TGG + contig192end:2811-2830 MsG0080048072.01.T01:intron 20.0%
!! CATTTCAACTAATAAACAAA+TGG - contig192end:1760-1779 None:intergenic 20.0%
!! CCTATAAACTTATATTTATG+GGG + contig192end:2813-2832 MsG0080048072.01.T01:intron 20.0%
!!! ATCTGTTCATTTTAAATTAG+TGG + contig192end:1430-1449 MsG0080048072.01.T01:intron 20.0%
!!! TAATTTATCAGCAACAATTT+TGG - contig192end:2274-2293 None:intergenic 20.0%
!!! TTTTCATGAATTCATATTAC+CGG - contig192end:2339-2358 None:intergenic 20.0%
! AACTATCATAAAAGAAGGAA+GGG - contig192end:1949-1968 None:intergenic 25.0%
! AAGATTGTCATGAAATTTCA+GGG + contig192end:2908-2927 MsG0080048072.01.T01:intron 25.0%
! ATCCAGATTTCAAAATTTCA+GGG - contig192end:1817-1836 None:intergenic 25.0%
! ATCGTTTAATTCTTCAGATA+AGG - contig192end:2854-2873 None:intergenic 25.0%
! ATTTCTCATCAAACATTTGT+AGG + contig192end:2673-2692 MsG0080048072.01.T01:intron 25.0%
! CCCCATAAATATAAGTTTAT+AGG - contig192end:2816-2835 None:intergenic 25.0%
! GAAAATGAAAACAGGAAATA+TGG + contig192end:1779-1798 MsG0080048072.01.T01:intron 25.0%
! GTTGAAATGAAAATGAAAAC+AGG + contig192end:1771-1790 MsG0080048072.01.T01:intron 25.0%
! TAAGATTGTCATGAAATTTC+AGG + contig192end:2907-2926 MsG0080048072.01.T01:intron 25.0%
! TAATCAAAACAATGCGTTAA+GGG - contig192end:3471-3490 None:intergenic 25.0%
! TAGGTGTGATAGAAATTTAT+AGG + contig192end:3391-3410 MsG0080048072.01.T01:CDS 25.0%
! TCATCAACTATCATAAAAGA+AGG - contig192end:1954-1973 None:intergenic 25.0%
! TGTATTGCTAAATATTCATG+TGG + contig192end:1177-1196 MsG0080048072.01.T01:intron 25.0%
! TTAGGGAAGAATGAATATTT+AGG + contig192end:1108-1127 MsG0080048072.01.T01:intron 25.0%
! TTCTTATCCTTTAACAGAAA+AGG - contig192end:3137-3156 None:intergenic 25.0%
! TTTCATGAATTCATATTACC+GGG - contig192end:2338-2357 None:intergenic 25.0%
! TTTGAACATTGAAGATGTAT+TGG - contig192end:1665-1684 None:intergenic 25.0%
!! CTAAAATCCTTTTCTGTTAA+AGG + contig192end:3127-3146 MsG0080048072.01.T01:intron 25.0%
!! GTTAGATCTATTTTCAGAAA+CGG + contig192end:1697-1716 MsG0080048072.01.T01:intron 25.0%
!! TGACATTTTCACTGTTAAAT+GGG + contig192end:1552-1571 MsG0080048072.01.T01:intron 25.0%
!! TGATGATAATGTATCTAAGA+AGG + contig192end:1981-2000 MsG0080048072.01.T01:intron 25.0%
!! TTCATTTTAAATTAGTGGCA+AGG + contig192end:1435-1454 MsG0080048072.01.T01:intron 25.0%
!! TTGACATTTTCACTGTTAAA+TGG + contig192end:1551-1570 MsG0080048072.01.T01:intron 25.0%
!! TTTTCTGTTAAAGGATAAGA+AGG + contig192end:3136-3155 MsG0080048072.01.T01:intron 25.0%
!!! AACAGAAAAGGATTTTAGTA+AGG - contig192end:3125-3144 None:intergenic 25.0%
!!! ATTATTTTGGTATTTGGATG+AGG + contig192end:838-857 MsG0080048072.01.T01:intron 25.0%
AATCAAAACAATGCGTTAAG+GGG - contig192end:3470-3489 None:intergenic 30.0%
AATCATATACTTCTGCAATG+TGG - contig192end:2585-2604 None:intergenic 30.0%
AATTCAGCTGGAAGAATATA+CGG + contig192end:2995-3014 MsG0080048072.01.T01:CDS 30.0%
AATTGGATCAAGAATCACAA+TGG - contig192end:3091-3110 None:intergenic 30.0%
ACACAAACAAGGTTTACTAA+AGG + contig192end:3045-3064 MsG0080048072.01.T01:CDS 30.0%
ACATACTGAATTAGTTGAAC+TGG + contig192end:1297-1316 MsG0080048072.01.T01:intron 30.0%
ACATGATTTGATTATTCTCC+CGG + contig192end:2317-2336 MsG0080048072.01.T01:CDS 30.0%
AGAATCACAATGGTAACAAA+AGG - contig192end:3081-3100 None:intergenic 30.0%
AGATAAGGACTACAATTTGA+AGG - contig192end:2839-2858 None:intergenic 30.0%
AGGAGTTCAATGAAATTGAT+TGG + contig192end:2001-2020 MsG0080048072.01.T01:intron 30.0%
ATAAATCAACACCAAAACCT+AGG - contig192end:1840-1859 None:intergenic 30.0%
ATAATTGTGTTCGAGAGAAA+TGG + contig192end:1480-1499 MsG0080048072.01.T01:intron 30.0%
CAACTATCATAAAAGAAGGA+AGG - contig192end:1950-1969 None:intergenic 30.0%
CTAATCAAAACAATGCGTTA+AGG - contig192end:3472-3491 None:intergenic 30.0%
CTTGAAGTCAACATTATAAC+TGG + contig192end:1136-1155 MsG0080048072.01.T01:intron 30.0%
GAATCACAATGGTAACAAAA+GGG - contig192end:3080-3099 None:intergenic 30.0%
GATCCAGATTTCAAAATTTC+AGG - contig192end:1818-1837 None:intergenic 30.0%
TCAGAAACGGTAAAAGAAAA+TGG + contig192end:1710-1729 MsG0080048072.01.T01:intron 30.0%
TGACACTGATAGATATTCTA+AGG + contig192end:2181-2200 MsG0080048072.01.T01:CDS 30.0%
TTACATGTTTGCTTTAACCT+TGG + contig192end:992-1011 MsG0080048072.01.T01:CDS 30.0%
TTATGCATTGTTTGCGATTA+GGG + contig192end:1091-1110 MsG0080048072.01.T01:intron 30.0%
TTTATGCATTGTTTGCGATT+AGG + contig192end:1090-1109 MsG0080048072.01.T01:intron 30.0%
TTTGATCTTATTGCTTTACC+TGG + contig192end:509-528 MsG0080048072.01.T01:CDS 30.0%
TTTGCCATCAATAACTACTA+CGG - contig192end:3216-3235 None:intergenic 30.0%
! ATTTTCAACTCTGCAGAATA+TGG - contig192end:3633-3652 None:intergenic 30.0%
! GAATTTTGCTTGGAATCTAA+AGG + contig192end:1371-1390 MsG0080048072.01.T01:intron 30.0%
! TGCAAATGATGAATTTTGCT+TGG + contig192end:1361-1380 MsG0080048072.01.T01:intron 30.0%
! TTTTCACTGTTAAATGGGAT+TGG + contig192end:1557-1576 MsG0080048072.01.T01:intron 30.0%
!! AATGAAATTGATTGGTCTGT+TGG + contig192end:2009-2028 MsG0080048072.01.T01:intron 30.0%
!!! AATTTTGAAATCTGGATCCT+AGG + contig192end:1820-1839 MsG0080048072.01.T01:intron 30.0%
!!! ATGAGAACTGACTTTTTTGA+TGG - contig192end:2435-2454 None:intergenic 30.0%
AAAATGAGATTCAGAGGACA+TGG - contig192end:3512-3531 None:intergenic 35.0%
AAATAGACTACTAGAGCATG+TGG + contig192end:3355-3374 MsG0080048072.01.T01:CDS 35.0%
AACGGTAAAAGAAAATGGTG+CGG + contig192end:1715-1734 MsG0080048072.01.T01:intron 35.0%
AATCACATGTAAACAAGCTG+AGG + contig192end:579-598 MsG0080048072.01.T01:intron 35.0%
AATCTTAGCACAAAGAACGA+TGG - contig192end:2894-2913 None:intergenic 35.0%
ACATGTATGTTATGCTAACC+AGG + contig192end:2116-2135 MsG0080048072.01.T01:intron 35.0%
ACATTATACATCTACCAGCT+TGG + contig192end:1618-1637 MsG0080048072.01.T01:intron 35.0%
ATAAGACAGTAACCTACACT+GGG - contig192end:2045-2064 None:intergenic 35.0%
ATCAAAACAATGCGTTAAGG+GGG - contig192end:3469-3488 None:intergenic 35.0%
ATCTTAGCACAAAGAACGAT+GGG - contig192end:2893-2912 None:intergenic 35.0%
ATTGTCATGAAATTTCAGGG+AGG + contig192end:2911-2930 MsG0080048072.01.T01:intron 35.0%
CAAACAATATAGCGAGTGTT+TGG - contig192end:815-834 None:intergenic 35.0%
CAAACATGCACGAAACAAAA+AGG - contig192end:689-708 None:intergenic 35.0%
CATCATTTGCATCAATCCTT+TGG - contig192end:1352-1371 None:intergenic 35.0%
CATTATACATCTACCAGCTT+GGG + contig192end:1619-1638 MsG0080048072.01.T01:intron 35.0%
CTCAAAGAACAAAATTCAGC+TGG + contig192end:2983-3002 MsG0080048072.01.T01:CDS 35.0%
GCATCAGATATCACAAAGAA+TGG - contig192end:1887-1906 None:intergenic 35.0%
GCTATTGTGAGCAAGTTATT+TGG + contig192end:3281-3300 MsG0080048072.01.T01:CDS 35.0%
GCTGAGGAAAATAGGTTATT+TGG + contig192end:595-614 MsG0080048072.01.T01:intron 35.0%
GGTGGAAAACAAAAATCCAA+AGG + contig192end:1333-1352 MsG0080048072.01.T01:intron 35.0%
GTAAACAAGCTGAGGAAAAT+AGG + contig192end:587-606 MsG0080048072.01.T01:intron 35.0%
GTAAGGCTTCACTAAAGAAT+TGG - contig192end:3108-3127 None:intergenic 35.0%
GTGAGCAAGTTATTTGGTAA+TGG + contig192end:3287-3306 MsG0080048072.01.T01:CDS 35.0%
GTTTATAGGTGCTTATGAAG+AGG - contig192end:2802-2821 None:intergenic 35.0%
GTTTCAGCTTAGTTGAACAT+TGG + contig192end:3598-3617 MsG0080048072.01.T01:three_prime_UTR 35.0%
TAAGTTTGTCAGAATCACAG+TGG - contig192end:784-803 None:intergenic 35.0%
TATAAGACAGTAACCTACAC+TGG - contig192end:2046-2065 None:intergenic 35.0%
TCAATTTGTAAGCCCTGAAT+TGG + contig192end:1200-1219 MsG0080048072.01.T01:intron 35.0%
TCCATGGACAATCTTAAAGA+CGG + contig192end:3176-3195 MsG0080048072.01.T01:CDS 35.0%
TCCTAGGATACTCAAAAACA+AGG - contig192end:3336-3355 None:intergenic 35.0%
TGCAGAGTTGAAAATATGCT+GGG + contig192end:3638-3657 MsG0080048072.01.T01:three_prime_UTR 35.0%
TGTTATCTTGCAAGACAAAC+TGG - contig192end:874-893 None:intergenic 35.0%
TTAAGATTGTCCATGGAAGA+GGG - contig192end:3173-3192 None:intergenic 35.0%
TTGTGATATCTGATGCATGT+TGG + contig192end:1891-1910 MsG0080048072.01.T01:intron 35.0%
! ATGTGGTTTCAAGTACTTGT+AGG + contig192end:3372-3391 MsG0080048072.01.T01:CDS 35.0%
! CATGTGATTTTGTTGAAGCT+CGG + contig192end:740-759 MsG0080048072.01.T01:intron 35.0%
! TTTAAGATTGTCCATGGAAG+AGG - contig192end:3174-3193 None:intergenic 35.0%
!! CTCTGAATCTCATTTTTCGA+GGG + contig192end:3516-3535 MsG0080048072.01.T01:three_prime_UTR 35.0%
!!! GACCCTGAAATTTTGAAATC+TGG + contig192end:1812-1831 MsG0080048072.01.T01:intron 35.0%
!!! GCTGCATTTTTTTTTGTCTG+TGG + contig192end:2369-2388 MsG0080048072.01.T01:intron 35.0%
ACACCACCTGAAAACAAATC+AGG - contig192end:3436-3455 None:intergenic 40.0%
ACCTGAAAACAAATCAGGCT+TGG - contig192end:3431-3450 None:intergenic 40.0%
ACGGTAAAAGAAAATGGTGC+GGG + contig192end:1716-1735 MsG0080048072.01.T01:intron 40.0%
ATGCGTTATCACACCAATTC+AGG - contig192end:1216-1235 None:intergenic 40.0%
ATTGGAATCCCACATTGATC+AGG - contig192end:1647-1666 None:intergenic 40.0%
CCTCGAAAAATGAGATTCAG+AGG - contig192end:3518-3537 None:intergenic 40.0%
CCTGAAAACAAATCAGGCTT+GGG - contig192end:3430-3449 None:intergenic 40.0%
CCTGCTATTCTACACAAACA+AGG + contig192end:3034-3053 MsG0080048072.01.T01:CDS 40.0%
CTGCAGAGTTGAAAATATGC+TGG + contig192end:3637-3656 MsG0080048072.01.T01:three_prime_UTR 40.0%
GAAAATGGTGCGGGAAAAAT+TGG + contig192end:1725-1744 MsG0080048072.01.T01:intron 40.0%
GAAGCTTGTGGAAAAATGCA+GGG - contig192end:901-920 None:intergenic 40.0%
GATCTTATTGCTTTACCTGG+AGG + contig192end:512-531 MsG0080048072.01.T01:CDS 40.0%
GATGCCGTAGTAGTTATTGA+TGG + contig192end:3209-3228 MsG0080048072.01.T01:CDS 40.0%
GCTCTAGTAGTCTATTTCCT+AGG - contig192end:3352-3371 None:intergenic 40.0%
GCTTCTGTTGAGAAAACTCT+TGG + contig192end:2228-2247 MsG0080048072.01.T01:CDS 40.0%
TAAGACAGTAACCTACACTG+GGG - contig192end:2044-2063 None:intergenic 40.0%
TGAACTGGAGAGTTTACGAA+GGG + contig192end:1312-1331 MsG0080048072.01.T01:intron 40.0%
TGAGAAAACTCTTGGAGTCT+TGG + contig192end:2236-2255 MsG0080048072.01.T01:CDS 40.0%
TGCGTTATCACACCAATTCA+GGG - contig192end:1215-1234 None:intergenic 40.0%
TTGAACTGGAGAGTTTACGA+AGG + contig192end:1311-1330 MsG0080048072.01.T01:intron 40.0%
TTGTGTTCGAGAGAAATGGA+AGG + contig192end:1484-1503 MsG0080048072.01.T01:intron 40.0%
TTTGTGTAGAATAGCAGGTG+AGG - contig192end:3032-3051 None:intergenic 40.0%
! CCTTGTTTGTGTAGAATAGC+AGG - contig192end:3037-3056 None:intergenic 40.0%
! GAAATCTGGATCCTAGGTTT+TGG + contig192end:1826-1845 MsG0080048072.01.T01:intron 40.0%
! GTCCAACATTCAGGTTCAAA+CGG + contig192end:939-958 MsG0080048072.01.T01:intron 40.0%
! GTTATATTTTGCACACCTGC+AGG - contig192end:3563-3582 None:intergenic 40.0%
! TCCAACATTCAGGTTCAAAC+GGG + contig192end:940-959 MsG0080048072.01.T01:intron 40.0%
! TGCTGTTACTCTTTTGCCTT+GGG + contig192end:636-655 MsG0080048072.01.T01:intron 40.0%
! TGGTGTGATAACGCATAAAC+CGG + contig192end:1220-1239 MsG0080048072.01.T01:intron 40.0%
!! CCTCTGAATCTCATTTTTCG+AGG + contig192end:3515-3534 MsG0080048072.01.T01:three_prime_UTR 40.0%
!! TCCGTCTTTAAGATTGTCCA+TGG - contig192end:3180-3199 None:intergenic 40.0%
!!! AAGCCTGATTTGTTTTCAGG+TGG + contig192end:3430-3449 MsG0080048072.01.T01:three_prime_UTR 40.0%
!!! ATGCTCTTTGAGCTAGCTTT+TGG - contig192end:1590-1609 None:intergenic 40.0%
!!! CTTTGAGCTAGCTTTTGGAT+GGG - contig192end:1585-1604 None:intergenic 40.0%
!!! GAATCTCATTTTTCGAGGGA+TGG + contig192end:3520-3539 MsG0080048072.01.T01:three_prime_UTR 40.0%
!!! GCCTTGTTTTTGAGTATCCT+AGG + contig192end:3332-3351 MsG0080048072.01.T01:CDS 40.0%
!!! TCTTTGAGCTAGCTTTTGGA+TGG - contig192end:1586-1605 None:intergenic 40.0%
AAAACAAATCAGGCTTGGGC+TGG - contig192end:3426-3445 None:intergenic 45.0%
AAACAAATCAGGCTTGGGCT+GGG - contig192end:3425-3444 None:intergenic 45.0%
AAATTTCAGGGAGGAACTGC+TGG + contig192end:2920-2939 MsG0080048072.01.T01:intron 45.0%
AACTTGAAGCTGAGGCTCAA+CGG - contig192end:426-445 None:intergenic 45.0%
AAGACAGTAACCTACACTGG+GGG - contig192end:2043-2062 None:intergenic 45.0%
AAGGGAGGTTGCTGAATTCT+TGG + contig192end:1043-1062 MsG0080048072.01.T01:CDS 45.0%
AGAGAAATGGAAGGCAGGTT+AGG + contig192end:1493-1512 MsG0080048072.01.T01:intron 45.0%
CAAACATGTAAGCAGTTCCC+GGG - contig192end:983-1002 None:intergenic 45.0%
CAAGGCAAAAGAGTAACAGC+AGG - contig192end:637-656 None:intergenic 45.0%
CAATGCAAACTCCGTTACAG+TGG - contig192end:3261-3280 None:intergenic 45.0%
CTGTTTGACCAGCTAGACTA+TGG - contig192end:1249-1268 None:intergenic 45.0%
CTTCTCTTCAAAAGACCCCA+AGG - contig192end:655-674 None:intergenic 45.0%
CTTGGGAACCTGATCAATGT+GGG + contig192end:1636-1655 MsG0080048072.01.T01:intron 45.0%
GATTCAGAGGACATGGTGAT+TGG - contig192end:3505-3524 None:intergenic 45.0%
GCAAACATGTAAGCAGTTCC+CGG - contig192end:984-1003 None:intergenic 45.0%
GCATAGGAAAAGTTACCTGC+AGG + contig192end:3545-3564 MsG0080048072.01.T01:three_prime_UTR 45.0%
GGAAGCTTGTGGAAAAATGC+AGG - contig192end:902-921 None:intergenic 45.0%
GGACTTAACAGCCAAAACGT+TGG - contig192end:923-942 None:intergenic 45.0%
GGCAAAGTGATCACTTGTGA+GGG + contig192end:3230-3249 MsG0080048072.01.T01:CDS 45.0%
GGCTGTTAAGTCCAACATTC+AGG + contig192end:930-949 MsG0080048072.01.T01:intron 45.0%
GTGGCTTCAAACATGAGAGT+TGG - contig192end:2566-2585 None:intergenic 45.0%
TCTCTCAATCTTGCTGAACC+AGG - contig192end:542-561 None:intergenic 45.0%
TGGCAAAGTGATCACTTGTG+AGG + contig192end:3229-3248 MsG0080048072.01.T01:CDS 45.0%
TTAGCACAAAGAACGATGGG+TGG - contig192end:2890-2909 None:intergenic 45.0%
! CACTGTAACGGAGTTTGCAT+TGG + contig192end:3259-3278 MsG0080048072.01.T01:CDS 45.0%
! CTGCTGTTACTCTTTTGCCT+TGG + contig192end:635-654 MsG0080048072.01.T01:intron 45.0%
! GCTGTTACTCTTTTGCCTTG+GGG + contig192end:637-656 MsG0080048072.01.T01:intron 45.0%
! TCCACAAGCTTCCAACGTTT+TGG + contig192end:909-928 MsG0080048072.01.T01:intron 45.0%
!! GGGGTCTTTTGAAGAGAAGA+AGG + contig192end:656-675 MsG0080048072.01.T01:intron 45.0%
!! TTTTCGAGGGATGGTTGCAT+AGG + contig192end:3529-3548 MsG0080048072.01.T01:three_prime_UTR 45.0%
!!! CCCAAGCCTGATTTGTTTTC+AGG + contig192end:3427-3446 MsG0080048072.01.T01:three_prime_UTR 45.0%
AACAAATCAGGCTTGGGCTG+GGG - contig192end:3424-3443 None:intergenic 50.0%
AATAGCAGGTGAGGAGCAGA+CGG - contig192end:3023-3042 None:intergenic 50.0%
ACCAGCTAGACTATGGCTTC+CGG - contig192end:1242-1261 None:intergenic 50.0%
ACTGGAGAGTTTACGAAGGG+TGG + contig192end:1315-1334 MsG0080048072.01.T01:intron 50.0%
AGACAGTAACCTACACTGGG+GGG - contig192end:2042-2061 None:intergenic 50.0%
AGATCCTCGTTGCAGAAGCA+TGG - contig192end:2095-2114 None:intergenic 50.0%
CAAGCTGCTGCATTTCTGTG+TGG + contig192end:2473-2492 MsG0080048072.01.T01:intron 50.0%
CATTGATCAGGTTCCCAAGC+TGG - contig192end:1635-1654 None:intergenic 50.0%
CTTCAGAACCATGCGCGATT+GGG - contig192end:2149-2168 None:intergenic 50.0%
CTTGGAGTCTTGGCTTCTCA+AGG + contig192end:2246-2265 MsG0080048072.01.T01:CDS 50.0%
GCAGCTACAACTTGAAGCTG+AGG - contig192end:434-453 None:intergenic 50.0%
GCCAAAACGTTGGAAGCTTG+TGG - contig192end:913-932 None:intergenic 50.0%
GCCCGTTTGAACCTGAATGT+TGG - contig192end:944-963 None:intergenic 50.0%
GCTTGGGAACCTGATCAATG+TGG + contig192end:1635-1654 MsG0080048072.01.T01:intron 50.0%
GCTTGTGGAAAAATGCAGGG+TGG - contig192end:898-917 None:intergenic 50.0%
GTTCGAGAGAAATGGAAGGC+AGG + contig192end:1488-1507 MsG0080048072.01.T01:intron 50.0%
GTTTGGGGAGTCTGTTGCAA+GGG + contig192end:1025-1044 MsG0080048072.01.T01:CDS 50.0%
TACAGCTCATCCCTCTTCCA+TGG + contig192end:3160-3179 MsG0080048072.01.T01:CDS 50.0%
TCTTCAGAACCATGCGCGAT+TGG - contig192end:2150-2169 None:intergenic 50.0%
TGAGGGACTTGCCACTGTAA+CGG + contig192end:3247-3266 MsG0080048072.01.T01:CDS 50.0%
TGTTTGGGGAGTCTGTTGCA+AGG + contig192end:1024-1043 MsG0080048072.01.T01:CDS 50.0%
TTGTCCATGCTTCTGCAACG+AGG + contig192end:2088-2107 MsG0080048072.01.T01:intron 50.0%
! GAAGCTCGGAAGTACACTTG+CGG + contig192end:754-773 MsG0080048072.01.T01:intron 50.0%
!! CAGCTTCAAGTTGTAGCTGC+TGG + contig192end:434-453 MsG0080048072.01.T01:CDS 50.0%
!! CTTCAAGTTGTAGCTGCTGG+AGG + contig192end:437-456 MsG0080048072.01.T01:CDS 50.0%
ACCGGAAGCCATAGTCTAGC+TGG + contig192end:1238-1257 MsG0080048072.01.T01:intron 55.0%
ACCTTGGCTGAGCAGCTGTT+TGG + contig192end:1008-1027 MsG0080048072.01.T01:CDS 55.0%
AGTAACCTACACTGGGGGGA+TGG - contig192end:2038-2057 None:intergenic 55.0%
CCTTGGCTGAGCAGCTGTTT+GGG + contig192end:1009-1028 MsG0080048072.01.T01:CDS 55.0%
CTGAGGCTCAACGGAAGCTA+CGG - contig192end:417-436 None:intergenic 55.0%
CTTGGCTGAGCAGCTGTTTG+GGG + contig192end:1010-1029 MsG0080048072.01.T01:CDS 55.0%
GCTTTACCTGGAGGAATGCC+TGG + contig192end:521-540 MsG0080048072.01.T01:CDS 55.0%
GTTCTCATCCCAATCGCGCA+TGG + contig192end:2138-2157 MsG0080048072.01.T01:CDS 55.0%
TGACATGAGCACCAGCACGA+CGG - contig192end:394-413 None:intergenic 55.0%
! AACGGGCTTACAACAAGCCG+TGG + contig192end:957-976 MsG0080048072.01.T01:CDS 55.0%
AACCTACACTGGGGGGATGG+TGG - contig192end:2035-2054 None:intergenic 60.0%
CCCAAACAGCTGCTCAGCCA+AGG - contig192end:1012-1031 None:intergenic 60.0%
CTTACAACAAGCCGTGGCCC+GGG + contig192end:963-982 MsG0080048072.01.T01:CDS 60.0%
GAGGAACTGCTGGCGCTCAA+AGG + contig192end:2930-2949 MsG0080048072.01.T01:CDS 60.0%
GCGCGATTGGGATGAGAACC+TGG - contig192end:2137-2156 None:intergenic 60.0%
GCTGAACCAGGCATTCCTCC+AGG - contig192end:530-549 None:intergenic 60.0%
GCTTACAACAAGCCGTGGCC+CGG + contig192end:962-981 MsG0080048072.01.T01:CDS 60.0%
TGGGGAGTCTGTTGCAAGGG+AGG + contig192end:1028-1047 MsG0080048072.01.T01:CDS 60.0%
TGTAAGCAGTTCCCGGGCCA+CGG - contig192end:977-996 None:intergenic 60.0%
! GCTCGGAAGTACACTTGCGG+TGG + contig192end:757-776 MsG0080048072.01.T01:intron 60.0%
ATCCACGTCCTCCGTCGTGC+TGG + contig192end:380-399 MsG0080048072.01.T01:CDS 65.0%
CATGAGCACCAGCACGACGG+AGG - contig192end:391-410 None:intergenic 65.0%
CACCAGCACGACGGAGGACG+TGG - contig192end:385-404 None:intergenic 70.0%
GGCCACCATCCCCCCAGTGT+AGG + contig192end:2030-2049 MsG0080048072.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
contig192end gene 362 3695 362 ID=MsG0080048072.01;Name=MsG0080048072.01
contig192end mRNA 362 3695 362 ID=MsG0080048072.01.T01;Parent=MsG0080048072.01;Name=MsG0080048072.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.5|0.6|1|1|1|5|283|298
contig192end exon 362 542 362 ID=MsG0080048072.01.T01:exon:933;Parent=MsG0080048072.01.T01
contig192end exon 952 1064 952 ID=MsG0080048072.01.T01:exon:934;Parent=MsG0080048072.01.T01
contig192end exon 2138 2338 2138 ID=MsG0080048072.01.T01:exon:935;Parent=MsG0080048072.01.T01
contig192end exon 2929 3066 2929 ID=MsG0080048072.01.T01:exon:936;Parent=MsG0080048072.01.T01
contig192end exon 3149 3695 3149 ID=MsG0080048072.01.T01:exon:937;Parent=MsG0080048072.01.T01
contig192end CDS 362 542 362 ID=MsG0080048072.01.T01:cds;Parent=MsG0080048072.01.T01
contig192end CDS 952 1064 952 ID=MsG0080048072.01.T01:cds;Parent=MsG0080048072.01.T01
contig192end CDS 2138 2338 2138 ID=MsG0080048072.01.T01:cds;Parent=MsG0080048072.01.T01
contig192end CDS 2929 3066 2929 ID=MsG0080048072.01.T01:cds;Parent=MsG0080048072.01.T01
contig192end CDS 3149 3412 3149 ID=MsG0080048072.01.T01:cds;Parent=MsG0080048072.01.T01
contig192end three_prime_UTR 3413 3695 3413 ID=MsG0080048072.01.T01:three_prime_utr;Parent=MsG0080048072.01.T01
Gene Sequence

>MsG0080048072.01.T01

ATGGAAGCAGTTATCTTAATCCACGTCCTCCGTCGTGCTGGTGCTCATGTCACCGTAGCTTCCGTTGAGCCTCAGCTTCAAGTTGTAGCTGCTGGAGGCACTAAATTAGTTGCTGATGCTTCTATCTCTGAGTGCTCTGATCAAATATTTGATCTTATTGCTTTACCTGGAGGAATGCCTGGTTCAAACGGGCTTACAACAAGCCGTGGCCCGGGAACTGCTTACATGTTTGCTTTAACCTTGGCTGAGCAGCTGTTTGGGGAGTCTGTTGCAAGGGAGGTTGCTGAATTCTTGGTTCTCATCCCAATCGCGCATGGTTCTGAAGAGATTGAAGTAGTGACACTGATAGATATTCTAAGGCGAGCAAAAGCTAATGTCGTAGTTGCTTCTGTTGAGAAAACTCTTGGAGTCTTGGCTTCTCAAGGAACCAAAATTGTTGCTGATAAATTAATTAGTGATATTCAAGAGTCAGCACATGATTTGATTATTCTCCCGGGAGGAACTGCTGGCGCTCAAAGGCTAAGCAAATCAAGAATTCTGAAGAAGCTTCTCAAAGAACAAAATTCAGCTGGAAGAATATACGGAGCCGTCTGCTCCTCACCTGCTATTCTACACAAACAAGGTTTACTAAAGGATAAGAAGGCTACAGCTCATCCCTCTTCCATGGACAATCTTAAAGACGGAGCAGTAAATGATGCCGTAGTAGTTATTGATGGCAAAGTGATCACTTGTGAGGGACTTGCCACTGTAACGGAGTTTGCATTGGCTATTGTGAGCAAGTTATTTGGTAATGGAAGAGCAAGAAGTGTAGCAGAAGCCTTGTTTTTGAGTATCCTAGGAAATAGACTACTAGAGCATGTGGTTTCAAGTACTTGTAGGTGTGATAGAAATTTATAG
Protein sequence

>MsG0080048072.01.T01

MEAVILIHVLRRAGAHVTVASVEPQLQVVAAGGTKLVADASISECSDQIFDLIALPGGMPGSNGLTTSRGPGTAYMFALTLAEQLFGESVAREVAEFLVLIPIAHGSEEIEVVTLIDILRRAKANVVVASVEKTLGVLASQGTKIVADKLISDIQESAHDLIILPGGTAGAQRLSKSRILKKLLKEQNSAGRIYGAVCSSPAILHKQGLLKDKKATAHPSSMDNLKDGAVNDAVVVIDGKVITCEGLATVTEFALAIVSKLFGNGRARSVAEALFLSILGNRLLEHVVSSTCRCDRNL*