Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000386.01.T01 | XP_003589034.1 | 82.524 | 206 | 29 | 1 | 1 | 199 | 136 | 341 | 9.64E-101 | 305 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000386.01.T01 | Q9FG61 | 49.254 | 201 | 94 | 2 | 1 | 193 | 191 | 391 | 4.24E-62 | 202 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000386.01.T01 | G7JMS8 | 82.524 | 206 | 29 | 1 | 1 | 199 | 136 | 341 | 4.60e-101 | 305 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000148.01 | MsG0180000386.01 | 0.803462 | 3.446652e-49 | 1.733100e-46 |
| MsG0180000385.01 | MsG0180000386.01 | 0.806134 | 9.532974e-50 | 5.134310e-47 |
| MsG0180000386.01 | MsG0180001533.01 | 0.815178 | 1.055869e-51 | 7.227540e-49 |
| MsG0180000386.01 | MsG0180003320.01 | 0.804283 | 2.326473e-49 | 1.194778e-46 |
| MsG0180000386.01 | MsG0180005353.01 | 0.823644 | 1.239775e-53 | 1.073770e-50 |
| MsG0180000386.01 | MsG0180005571.01 | 0.816963 | 4.216902e-52 | 3.031559e-49 |
| MsG0180000386.01 | MsG0280009167.01 | 0.801121 | 1.045484e-48 | 4.953245e-46 |
| MsG0180000386.01 | MsG0280010410.01 | 0.801116 | 1.047850e-48 | 4.963847e-46 |
| MsG0180000386.01 | MsG0280010802.01 | 0.813615 | 2.339554e-51 | 1.535241e-48 |
| MsG0180000386.01 | MsG0280010936.01 | 0.802128 | 6.496826e-49 | 3.157444e-46 |
| MsG0180000386.01 | MsG0380011635.01 | 0.811337 | 7.363079e-51 | 4.545393e-48 |
| MsG0180000386.01 | MsG0380011974.01 | 0.821177 | 4.637048e-53 | 3.745447e-50 |
| MsG0180000386.01 | MsG0380013285.01 | 0.803462 | 3.446043e-49 | 1.732813e-46 |
| MsG0180000386.01 | MsG0380016045.01 | 0.800607 | 1.331504e-48 | 6.226451e-46 |
| MsG0180000386.01 | MsG0380016299.01 | 0.801229 | 9.934154e-49 | 4.719303e-46 |
| MsG0180000386.01 | MsG0480018439.01 | 0.804371 | 2.230517e-49 | 1.148104e-46 |
| MsG0180000386.01 | MsG0480022833.01 | 0.804791 | 1.823080e-49 | 9.486334e-47 |
| MsG0180000386.01 | MsG0580024990.01 | 0.803111 | 4.073703e-49 | 2.029933e-46 |
| MsG0180000386.01 | MsG0580026492.01 | 0.818513 | 1.885207e-52 | 1.414077e-49 |
| MsG0180000386.01 | MsG0580026521.01 | 0.807089 | 5.993494e-50 | 3.308013e-47 |
| MsG0180000386.01 | MsG0580029938.01 | 0.829039 | 6.434661e-55 | 6.508237e-52 |
| MsG0180000386.01 | MsG0580030135.01 | 0.801109 | 1.051613e-48 | 4.980796e-46 |
| MsG0180000386.01 | MsG0680030613.01 | 0.801686 | 8.007324e-49 | 3.847965e-46 |
| MsG0180000386.01 | MsG0680031009.01 | 0.800265 | 1.562753e-48 | 7.244929e-46 |
| MsG0180000386.01 | MsG0680034316.01 | 0.800982 | 1.116125e-48 | 5.269577e-46 |
| MsG0180000386.01 | MsG0780036376.01 | 0.803263 | 3.788750e-49 | 1.895390e-46 |
| MsG0180000386.01 | MsG0780036472.01 | 0.804076 | 2.569314e-49 | 1.312537e-46 |
| MsG0180000386.01 | MsG0780038519.01 | 0.813134 | 2.984224e-51 | 1.932676e-48 |
| MsG0180000386.01 | MsG0780038922.01 | 0.816209 | 6.220738e-52 | 4.380602e-49 |
| MsG0180000386.01 | MsG0780040991.01 | 0.801088 | 1.061739e-48 | 5.026290e-46 |
| MsG0180000386.01 | MsG0880046271.01 | 0.800750 | 1.244913e-48 | 5.842562e-46 |
| MsG0180000386.01 | MsG0880047714.01 | 0.803727 | 3.036796e-49 | 1.537430e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000386.01.T01 | MTR_1g016620 | 82.524 | 206 | 29 | 1 | 1 | 199 | 136 | 341 | 1.17e-104 | 305 |
| MsG0180000386.01.T01 | MTR_7g080170 | 47.115 | 208 | 102 | 2 | 1 | 200 | 83 | 290 | 6.62e-60 | 192 |
| MsG0180000386.01.T01 | MTR_7g021530 | 49.744 | 195 | 89 | 3 | 1 | 187 | 125 | 318 | 4.10e-59 | 189 |
| MsG0180000386.01.T01 | MTR_7g021530 | 49.744 | 195 | 89 | 3 | 1 | 187 | 175 | 368 | 1.42e-58 | 189 |
| MsG0180000386.01.T01 | MTR_7g080170 | 47.115 | 208 | 102 | 2 | 1 | 200 | 191 | 398 | 1.96e-58 | 191 |
| MsG0180000386.01.T01 | MTR_7g021530 | 50.000 | 194 | 88 | 3 | 1 | 186 | 216 | 408 | 4.68e-58 | 189 |
| MsG0180000386.01.T01 | MTR_4g037470 | 49.239 | 197 | 92 | 2 | 1 | 189 | 177 | 373 | 2.73e-57 | 187 |
| MsG0180000386.01.T01 | MTR_4g037470 | 49.239 | 197 | 92 | 2 | 1 | 189 | 186 | 382 | 2.93e-57 | 187 |
| MsG0180000386.01.T01 | MTR_7g070510 | 46.701 | 197 | 98 | 2 | 1 | 190 | 172 | 368 | 8.06e-57 | 186 |
| MsG0180000386.01.T01 | MTR_2g090190 | 49.746 | 197 | 91 | 2 | 1 | 189 | 170 | 366 | 1.08e-55 | 183 |
| MsG0180000386.01.T01 | MTR_8g017240 | 47.179 | 195 | 93 | 3 | 1 | 187 | 148 | 340 | 8.37e-50 | 166 |
| MsG0180000386.01.T01 | MTR_1g083690 | 42.051 | 195 | 105 | 1 | 1 | 187 | 157 | 351 | 6.17e-46 | 155 |
| MsG0180000386.01.T01 | MTR_4g013295 | 40.865 | 208 | 102 | 3 | 1 | 193 | 149 | 350 | 6.48e-46 | 155 |
| MsG0180000386.01.T01 | MTR_4g120410 | 37.438 | 203 | 119 | 2 | 1 | 195 | 159 | 361 | 7.31e-44 | 150 |
| MsG0180000386.01.T01 | MTR_2g435550 | 38.000 | 200 | 116 | 1 | 1 | 192 | 146 | 345 | 1.45e-41 | 144 |
| MsG0180000386.01.T01 | MTR_7g112490 | 38.806 | 201 | 104 | 2 | 1 | 187 | 167 | 362 | 1.85e-41 | 144 |
| MsG0180000386.01.T01 | MTR_2g435550 | 38.000 | 200 | 116 | 1 | 1 | 192 | 160 | 359 | 1.92e-41 | 144 |
| MsG0180000386.01.T01 | MTR_7g112490 | 38.806 | 201 | 104 | 2 | 1 | 187 | 185 | 380 | 2.24e-41 | 144 |
| MsG0180000386.01.T01 | MTR_7g021530 | 48.630 | 146 | 67 | 2 | 1 | 138 | 175 | 320 | 5.44e-40 | 139 |
| MsG0180000386.01.T01 | MTR_7g021530 | 48.630 | 146 | 67 | 2 | 1 | 138 | 216 | 361 | 1.43e-39 | 139 |
| MsG0180000386.01.T01 | MTR_2g435550 | 39.429 | 175 | 98 | 1 | 1 | 167 | 160 | 334 | 1.21e-35 | 129 |
| MsG0180000386.01.T01 | MTR_6g087000 | 28.571 | 217 | 124 | 6 | 11 | 198 | 180 | 394 | 9.57e-19 | 84.0 |
| MsG0180000386.01.T01 | MTR_6g087000 | 28.571 | 217 | 124 | 6 | 11 | 198 | 180 | 394 | 1.16e-18 | 84.0 |
| MsG0180000386.01.T01 | MTR_4g118340 | 26.601 | 203 | 112 | 5 | 10 | 182 | 141 | 336 | 4.90e-18 | 81.3 |
| MsG0180000386.01.T01 | MTR_3g105730 | 41.414 | 99 | 49 | 3 | 92 | 189 | 194 | 284 | 3.38e-15 | 72.8 |
| MsG0180000386.01.T01 | MTR_3g105730 | 41.414 | 99 | 49 | 3 | 92 | 189 | 172 | 262 | 4.42e-15 | 72.0 |
| MsG0180000386.01.T01 | MTR_1g014640 | 39.450 | 109 | 57 | 3 | 92 | 199 | 237 | 337 | 1.11e-14 | 71.6 |
| MsG0180000386.01.T01 | MTR_1g013400 | 39.450 | 109 | 57 | 3 | 92 | 199 | 237 | 337 | 1.11e-14 | 71.6 |
| MsG0180000386.01.T01 | MTR_4g094542 | 35.156 | 128 | 72 | 4 | 38 | 165 | 234 | 350 | 4.60e-14 | 70.1 |
| MsG0180000386.01.T01 | MTR_4g094208 | 32.520 | 123 | 67 | 4 | 56 | 163 | 139 | 260 | 3.65e-13 | 66.6 |
| MsG0180000386.01.T01 | MTR_2g020970 | 25.118 | 211 | 120 | 6 | 2 | 182 | 135 | 337 | 2.82e-12 | 64.7 |
| MsG0180000386.01.T01 | MTR_2g020970 | 25.118 | 211 | 120 | 6 | 2 | 182 | 135 | 337 | 3.39e-12 | 64.7 |
| MsG0180000386.01.T01 | MTR_1g115570 | 34.646 | 127 | 69 | 4 | 38 | 163 | 208 | 321 | 1.49e-11 | 62.8 |
| MsG0180000386.01.T01 | MTR_2g008850 | 28.276 | 145 | 82 | 5 | 56 | 185 | 97 | 234 | 1.71e-11 | 61.6 |
| MsG0180000386.01.T01 | MTR_2g008850 | 29.268 | 123 | 71 | 4 | 56 | 163 | 143 | 264 | 2.82e-11 | 61.6 |
| MsG0180000386.01.T01 | MTR_8g463130 | 31.707 | 123 | 68 | 4 | 56 | 163 | 144 | 265 | 3.07e-11 | 61.2 |
| MsG0180000386.01.T01 | MTR_8g463130 | 31.707 | 123 | 68 | 4 | 56 | 163 | 116 | 237 | 3.34e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 96 | 290 | 4.14e-63 | 201 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 96 | 290 | 4.14e-63 | 201 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 96 | 290 | 4.14e-63 | 201 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 96 | 290 | 4.14e-63 | 201 |
| MsG0180000386.01.T01 | AT5G36250 | 49.254 | 201 | 94 | 2 | 1 | 193 | 191 | 391 | 4.32e-63 | 202 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 136 | 330 | 2.00e-62 | 200 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 136 | 330 | 2.00e-62 | 200 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 206 | 400 | 6.92e-62 | 200 |
| MsG0180000386.01.T01 | AT1G79630 | 50.769 | 195 | 88 | 2 | 1 | 187 | 202 | 396 | 7.96e-62 | 200 |
| MsG0180000386.01.T01 | AT1G16220 | 50.256 | 195 | 89 | 2 | 1 | 187 | 183 | 377 | 4.15e-61 | 198 |
| MsG0180000386.01.T01 | AT4G32950 | 51.485 | 202 | 87 | 5 | 1 | 191 | 122 | 323 | 9.99e-60 | 190 |
| MsG0180000386.01.T01 | AT5G36250 | 46.512 | 215 | 93 | 3 | 1 | 193 | 191 | 405 | 2.63e-59 | 192 |
| MsG0180000386.01.T01 | AT3G02750 | 51.244 | 201 | 88 | 4 | 1 | 192 | 200 | 399 | 1.18e-56 | 186 |
| MsG0180000386.01.T01 | AT3G02750 | 51.244 | 201 | 88 | 4 | 1 | 192 | 200 | 399 | 1.18e-56 | 186 |
| MsG0180000386.01.T01 | AT1G03590 | 50.761 | 197 | 89 | 2 | 1 | 189 | 169 | 365 | 2.38e-56 | 185 |
| MsG0180000386.01.T01 | AT4G03415 | 48.731 | 197 | 93 | 2 | 1 | 189 | 179 | 375 | 1.63e-54 | 180 |
| MsG0180000386.01.T01 | AT4G03415 | 48.731 | 197 | 93 | 2 | 1 | 189 | 179 | 375 | 1.63e-54 | 180 |
| MsG0180000386.01.T01 | AT4G03415 | 48.731 | 197 | 93 | 2 | 1 | 189 | 179 | 375 | 1.63e-54 | 180 |
| MsG0180000386.01.T01 | AT5G01700 | 44.444 | 207 | 104 | 4 | 1 | 200 | 96 | 298 | 2.42e-51 | 168 |
| MsG0180000386.01.T01 | AT3G02750 | 43.830 | 235 | 89 | 4 | 1 | 192 | 200 | 434 | 2.43e-51 | 173 |
| MsG0180000386.01.T01 | AT5G01700 | 44.444 | 207 | 104 | 4 | 1 | 200 | 145 | 347 | 6.90e-51 | 169 |
| MsG0180000386.01.T01 | AT5G26010 | 51.000 | 200 | 88 | 4 | 1 | 191 | 131 | 329 | 1.46e-50 | 166 |
| MsG0180000386.01.T01 | AT3G05640 | 38.500 | 200 | 105 | 2 | 1 | 187 | 156 | 350 | 8.61e-42 | 144 |
| MsG0180000386.01.T01 | AT3G05640 | 38.500 | 200 | 105 | 2 | 1 | 187 | 156 | 350 | 8.61e-42 | 144 |
| MsG0180000386.01.T01 | AT3G05640 | 38.500 | 200 | 105 | 2 | 1 | 187 | 156 | 350 | 8.61e-42 | 144 |
| MsG0180000386.01.T01 | AT5G27930 | 37.879 | 198 | 105 | 2 | 1 | 185 | 161 | 353 | 1.12e-41 | 144 |
| MsG0180000386.01.T01 | AT5G27930 | 37.879 | 198 | 105 | 2 | 1 | 185 | 161 | 353 | 1.12e-41 | 144 |
| MsG0180000386.01.T01 | AT5G36250 | 47.222 | 144 | 68 | 2 | 1 | 136 | 191 | 334 | 9.34e-40 | 139 |
| MsG0180000386.01.T01 | AT3G16800 | 36.224 | 196 | 116 | 2 | 1 | 187 | 59 | 254 | 3.88e-38 | 132 |
| MsG0180000386.01.T01 | AT3G16800 | 36.224 | 196 | 116 | 2 | 1 | 187 | 59 | 254 | 3.88e-38 | 132 |
| MsG0180000386.01.T01 | AT3G16800 | 36.224 | 196 | 116 | 2 | 1 | 187 | 154 | 349 | 1.68e-37 | 133 |
| MsG0180000386.01.T01 | AT3G16800 | 36.224 | 196 | 116 | 2 | 1 | 187 | 154 | 349 | 1.68e-37 | 133 |
| MsG0180000386.01.T01 | AT3G16800 | 36.224 | 196 | 116 | 2 | 1 | 187 | 154 | 349 | 1.68e-37 | 133 |
| MsG0180000386.01.T01 | AT4G32950 | 48.592 | 142 | 64 | 4 | 1 | 133 | 122 | 263 | 1.96e-35 | 126 |
| MsG0180000386.01.T01 | AT5G27930 | 36.301 | 146 | 75 | 2 | 1 | 133 | 161 | 301 | 2.04e-26 | 103 |
| MsG0180000386.01.T01 | AT2G20050 | 28.934 | 197 | 113 | 5 | 11 | 182 | 199 | 393 | 6.68e-18 | 81.6 |
| MsG0180000386.01.T01 | AT2G20050 | 28.934 | 197 | 113 | 5 | 11 | 182 | 199 | 393 | 6.75e-18 | 81.6 |
| MsG0180000386.01.T01 | AT3G06270 | 25.688 | 218 | 124 | 6 | 1 | 188 | 135 | 344 | 4.58e-16 | 75.5 |
| MsG0180000386.01.T01 | AT5G24940 | 42.268 | 97 | 47 | 3 | 92 | 187 | 193 | 281 | 2.31e-14 | 71.2 |
| MsG0180000386.01.T01 | AT4G31750 | 43.158 | 95 | 46 | 3 | 92 | 185 | 193 | 280 | 3.07e-14 | 70.1 |
| MsG0180000386.01.T01 | AT4G31750 | 43.158 | 95 | 46 | 3 | 92 | 185 | 193 | 280 | 3.07e-14 | 70.1 |
| MsG0180000386.01.T01 | AT4G31750 | 43.158 | 95 | 46 | 3 | 92 | 185 | 193 | 280 | 4.32e-14 | 69.7 |
| MsG0180000386.01.T01 | AT5G10740 | 31.250 | 192 | 106 | 7 | 2 | 187 | 110 | 281 | 4.14e-13 | 67.4 |
| MsG0180000386.01.T01 | AT5G10740 | 31.250 | 192 | 106 | 7 | 2 | 187 | 110 | 281 | 4.14e-13 | 67.4 |
| MsG0180000386.01.T01 | AT4G28400 | 31.783 | 129 | 72 | 4 | 56 | 169 | 123 | 250 | 4.54e-13 | 66.2 |
| MsG0180000386.01.T01 | AT4G28400 | 31.783 | 129 | 72 | 3 | 56 | 169 | 145 | 272 | 5.32e-13 | 66.2 |
| MsG0180000386.01.T01 | AT2G20630 | 30.573 | 157 | 83 | 6 | 56 | 195 | 141 | 288 | 1.08e-12 | 65.5 |
| MsG0180000386.01.T01 | AT2G20630 | 32.000 | 125 | 69 | 3 | 56 | 165 | 141 | 264 | 3.53e-12 | 63.9 |
| MsG0180000386.01.T01 | AT2G34740 | 40.206 | 97 | 49 | 3 | 92 | 187 | 248 | 336 | 8.60e-12 | 63.5 |
| MsG0180000386.01.T01 | AT1G22280 | 32.520 | 123 | 67 | 4 | 56 | 163 | 149 | 270 | 2.93e-11 | 61.6 |
| MsG0180000386.01.T01 | AT1G43900 | 36.000 | 125 | 63 | 4 | 56 | 165 | 232 | 354 | 3.32e-11 | 61.6 |
| MsG0180000386.01.T01 | AT1G22280 | 32.520 | 123 | 67 | 4 | 56 | 163 | 143 | 264 | 3.67e-11 | 61.2 |
Find 52 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTGTATCTAAACTAAAT+TGG | 0.214115 | 1:+5312451 | MsG0180000386.01.T01:CDS |
| CGGTTGTGTTTATGCGTTAA+AGG | 0.236530 | 1:+5312509 | MsG0180000386.01.T01:CDS |
| TTATTGCTACACCAGATATT+TGG | 0.238563 | 1:+5312631 | MsG0180000386.01.T01:CDS |
| ACCACAAAACTTGGAGTATA+TGG | 0.271089 | 1:+5312958 | MsG0180000386.01.T01:CDS |
| GTATATGGACAGTGGAAATT+AGG | 0.308125 | 1:+5312973 | MsG0180000386.01.T01:CDS |
| CAGGCGAAGCAAAGAGAATA+AGG | 0.327044 | 1:+5312478 | MsG0180000386.01.T01:CDS |
| CTAGCCATGTCTCGAGCTTT+CGG | 0.377337 | 1:+5312582 | MsG0180000386.01.T01:CDS |
| AAACGGTGATACCAAATATC+TGG | 0.385349 | 1:-5312642 | None:intergenic |
| GTGGTTTCTTCTGCAGGAAA+TGG | 0.397763 | 1:-5312940 | None:intergenic |
| CACTTGCAAGAACAATAAAT+TGG | 0.399587 | 1:-5312677 | None:intergenic |
| TAGGCCAGGGTAGTTCTCAT+TGG | 0.416310 | 1:-5312562 | None:intergenic |
| ATTGGTCACTTGATGTTAAA+CGG | 0.419251 | 1:-5312659 | None:intergenic |
| AACCACATGTCCAACGAGTA+TGG | 0.432459 | 1:+5312535 | MsG0180000386.01.T01:CDS |
| TTTATTGTTCTTGCAAGTGA+TGG | 0.439321 | 1:+5312681 | MsG0180000386.01.T01:CDS |
| GCAGAAGAAACCACAAAACT+TGG | 0.461758 | 1:+5312949 | MsG0180000386.01.T01:CDS |
| CTTCTTTGCCCATGCAGCTG+TGG | 0.475385 | 1:-5312879 | None:intergenic |
| AGGCCAGGGTAGTTCTCATT+GGG | 0.484559 | 1:-5312561 | None:intergenic |
| TTATTGTTCTTGCAAGTGAT+GGG | 0.484730 | 1:+5312682 | MsG0180000386.01.T01:CDS |
| GGAAGCAGCCACAGCTGCAT+GGG | 0.485485 | 1:+5312871 | MsG0180000386.01.T01:CDS |
| TCTAAACTAAATTGGAATGC+AGG | 0.486606 | 1:+5312459 | MsG0180000386.01.T01:CDS |
| GCTCGAGACATGGCTAGGCC+AGG | 0.488145 | 1:-5312576 | None:intergenic |
| GGAAGCTGCAAGGGCAGTGG+TGG | 0.495822 | 1:+5312850 | MsG0180000386.01.T01:CDS |
| GAGTGAAGAGGAAGCTGCAA+GGG | 0.496594 | 1:+5312841 | MsG0180000386.01.T01:CDS |
| CAAAGAAGTTTCCTTCCTCT+AGG | 0.507704 | 1:+5312894 | MsG0180000386.01.T01:CDS |
| TGCTGTAGTTGTCATAAGAC+AGG | 0.527202 | 1:+5311645 | MsG0180000386.01.T01:CDS |
| TGGAAGCAGCCACAGCTGCA+TGG | 0.533129 | 1:+5312870 | MsG0180000386.01.T01:CDS |
| AATCTAGATTGTTCTTGTAG+TGG | 0.537196 | 1:+5311616 | MsG0180000386.01.T01:CDS |
| AAGAGCGATGCTATTTCTAA+CGG | 0.548719 | 1:-5312424 | None:intergenic |
| AAAGAAGTTTCCTTCCTCTA+GGG | 0.562358 | 1:+5312895 | MsG0180000386.01.T01:CDS |
| AACCATACTCGTTGGACATG+TGG | 0.563398 | 1:-5312537 | None:intergenic |
| CATTGGGCAACCATACTCGT+TGG | 0.565790 | 1:-5312545 | None:intergenic |
| CGAAAGCTCGAGACATGGCT+AGG | 0.567459 | 1:-5312581 | None:intergenic |
| TTTCAATGTAATGGATGAAG+AGG | 0.572129 | 1:+5311579 | None:intergenic |
| AGAGGAAGCTGCAAGGGCAG+TGG | 0.574488 | 1:+5312847 | MsG0180000386.01.T01:CDS |
| CTCGAGACATGGCTAGGCCA+GGG | 0.576527 | 1:-5312575 | None:intergenic |
| TTGCCCAATGAGAACTACCC+TGG | 0.599999 | 1:+5312558 | MsG0180000386.01.T01:CDS |
| CAATCATCTACCCTAGAGGA+AGG | 0.600898 | 1:-5312905 | None:intergenic |
| AGAGTGAAGAGGAAGCTGCA+AGG | 0.613435 | 1:+5312840 | MsG0180000386.01.T01:CDS |
| TATTGTTCTTGCAAGTGATG+GGG | 0.615238 | 1:+5312683 | MsG0180000386.01.T01:CDS |
| ATCTCCGAAAGCTCGAGACA+TGG | 0.616410 | 1:-5312586 | None:intergenic |
| GGTTGCATCAATTGTGTGGA+TGG | 0.622975 | 1:+5312814 | MsG0180000386.01.T01:CDS |
| GATTTCATACTTAAAGACCA+TGG | 0.635074 | 1:+5312606 | MsG0180000386.01.T01:CDS |
| GTGGATGGTAGAGAGTGAAG+AGG | 0.644994 | 1:+5312829 | MsG0180000386.01.T01:CDS |
| AAGAGGTTGCATCAATTGTG+TGG | 0.658363 | 1:+5312810 | MsG0180000386.01.T01:CDS |
| AAGAGAATAAGGAGTTGCAA+CGG | 0.664540 | 1:+5312489 | MsG0180000386.01.T01:CDS |
| AACTTGGAGTATATGGACAG+TGG | 0.675658 | 1:+5312965 | MsG0180000386.01.T01:CDS |
| GGATGTACTAAGTAATGAAG+AGG | 0.676441 | 1:+5312793 | MsG0180000386.01.T01:CDS |
| CTGCAGGAAATGGCAAACCA+CGG | 0.691531 | 1:-5312930 | None:intergenic |
| ATAGACTAGTGAAACTGCAG+CGG | 0.692728 | 1:+5312383 | MsG0180000386.01.T01:intron |
| GGTGCAATCATCTACCCTAG+AGG | 0.703711 | 1:-5312909 | None:intergenic |
| CTGGTGTAGCAATAACACCA+TGG | 0.708013 | 1:-5312623 | None:intergenic |
| TAGGGTAGATGATTGCACCG+TGG | 0.761662 | 1:+5312913 | MsG0180000386.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTAAATATTATTATATT+TGG | - | Chr1:5312232-5312251 | None:intergenic | 0.0% |
| !!! | ATCATTTTCTAAAAAAAAAA+AGG | + | Chr1:5312164-5312183 | MsG0180000386.01.T01:intron | 10.0% |
| !! | TATTTGTATCTAAACTAAAT+TGG | + | Chr1:5312451-5312470 | MsG0180000386.01.T01:CDS | 15.0% |
| !!! | ATTTTCTAAAAAAAAAAAGG+TGG | + | Chr1:5312167-5312186 | MsG0180000386.01.T01:intron | 15.0% |
| !! | ATAACAAGAACAATATAAAC+AGG | + | Chr1:5311729-5311748 | MsG0180000386.01.T01:intron | 20.0% |
| !! | ATATTTGGATTAAAATACGT+CGG | - | Chr1:5312217-5312236 | None:intergenic | 20.0% |
| !! | TAACAAGAACAATATAAACA+GGG | + | Chr1:5311730-5311749 | MsG0180000386.01.T01:intron | 20.0% |
| !! | TTCATATAACTGTTAAACTA+AGG | - | Chr1:5312090-5312109 | None:intergenic | 20.0% |
| !!! | AATGGCTTTATCATTATTTA+TGG | + | Chr1:5312748-5312767 | MsG0180000386.01.T01:intron | 20.0% |
| !!! | TATGGATATTTTTTGCTTAT+AGG | + | Chr1:5312766-5312785 | MsG0180000386.01.T01:intron | 20.0% |
| ! | TTTATGAGAAGAATCTTACA+AGG | - | Chr1:5311863-5311882 | None:intergenic | 25.0% |
| !!! | ATATTTTTTGCTTATAGGTG+TGG | + | Chr1:5312771-5312790 | MsG0180000386.01.T01:intron | 25.0% |
| !!! | CTTTTCAATTATTGGTTTGA+CGG | + | Chr1:5311897-5311916 | MsG0180000386.01.T01:intron | 25.0% |
| !!! | TATTTTTTGCTTATAGGTGT+GGG | + | Chr1:5312772-5312791 | MsG0180000386.01.T01:intron | 25.0% |
| AACTGTTAAACTAAGGTGTA+TGG | - | Chr1:5312083-5312102 | None:intergenic | 30.0% | |
| AATCTAGATTGTTCTTGTAG+TGG | + | Chr1:5311616-5311635 | MsG0180000386.01.T01:CDS | 30.0% | |
| ATAATTGAAAAGAAGCTGCA+TGG | - | Chr1:5311889-5311908 | None:intergenic | 30.0% | |
| ATTGGTCACTTGATGTTAAA+CGG | - | Chr1:5312662-5312681 | None:intergenic | 30.0% | |
| CAAATAAGAGACTTCTAGAT+AGG | + | Chr1:5312138-5312157 | MsG0180000386.01.T01:intron | 30.0% | |
| CACTTGCAAGAACAATAAAT+TGG | - | Chr1:5312680-5312699 | None:intergenic | 30.0% | |
| CTTGCATACAACAATTTCAA+TGG | + | Chr1:5312730-5312749 | MsG0180000386.01.T01:intron | 30.0% | |
| GATTTCATACTTAAAGACCA+TGG | + | Chr1:5312606-5312625 | MsG0180000386.01.T01:CDS | 30.0% | |
| TAAACGTCAATAATGACACA+GGG | + | Chr1:5312286-5312305 | MsG0180000386.01.T01:intron | 30.0% | |
| TCTAAACTAAATTGGAATGC+AGG | + | Chr1:5312459-5312478 | MsG0180000386.01.T01:CDS | 30.0% | |
| TTAAACGTCAATAATGACAC+AGG | + | Chr1:5312285-5312304 | MsG0180000386.01.T01:intron | 30.0% | |
| TTATTGCTACACCAGATATT+TGG | + | Chr1:5312631-5312650 | MsG0180000386.01.T01:CDS | 30.0% | |
| TTATTGTTCTTGCAAGTGAT+GGG | + | Chr1:5312682-5312701 | MsG0180000386.01.T01:CDS | 30.0% | |
| TTTATTGTTCTTGCAAGTGA+TGG | + | Chr1:5312681-5312700 | MsG0180000386.01.T01:CDS | 30.0% | |
| ! | TGCAGCTTCTTTTCAATTAT+TGG | + | Chr1:5311889-5311908 | MsG0180000386.01.T01:intron | 30.0% |
| ! | TGTGTCATTATTGACGTTTA+AGG | - | Chr1:5312286-5312305 | None:intergenic | 30.0% |
| !!! | TGGCTTTTAGTTTTTCATCT+TGG | - | Chr1:5311809-5311828 | None:intergenic | 30.0% |
| AAACGGTGATACCAAATATC+TGG | - | Chr1:5312645-5312664 | None:intergenic | 35.0% | |
| AAAGAAGTTTCCTTCCTCTA+GGG | + | Chr1:5312895-5312914 | MsG0180000386.01.T01:CDS | 35.0% | |
| AAGAATCTTACAAGGCAATC+CGG | - | Chr1:5311855-5311874 | None:intergenic | 35.0% | |
| AAGAGAATAAGGAGTTGCAA+CGG | + | Chr1:5312489-5312508 | MsG0180000386.01.T01:CDS | 35.0% | |
| ACCACAAAACTTGGAGTATA+TGG | + | Chr1:5312958-5312977 | MsG0180000386.01.T01:CDS | 35.0% | |
| ATAAGAGACTTCTAGATAGG+TGG | + | Chr1:5312141-5312160 | MsG0180000386.01.T01:intron | 35.0% | |
| CAAATCAGTGGTCAATTGAA+TGG | - | Chr1:5311829-5311848 | None:intergenic | 35.0% | |
| GAACAATATAAACAGGGTGA+AGG | + | Chr1:5311736-5311755 | MsG0180000386.01.T01:intron | 35.0% | |
| GGATGTACTAAGTAATGAAG+AGG | + | Chr1:5312793-5312812 | MsG0180000386.01.T01:CDS | 35.0% | |
| TATTGTTCTTGCAAGTGATG+GGG | + | Chr1:5312683-5312702 | MsG0180000386.01.T01:CDS | 35.0% | |
| ! | GGTCTTATCATAGTTAACCT+TGG | + | Chr1:5311757-5311776 | MsG0180000386.01.T01:intron | 35.0% |
| ! | TCCATATACTCCAAGTTTTG+TGG | - | Chr1:5312962-5312981 | None:intergenic | 35.0% |
| !! | AAGAGCGATGCTATTTCTAA+CGG | - | Chr1:5312427-5312446 | None:intergenic | 35.0% |
| AACTTGGAGTATATGGACAG+TGG | + | Chr1:5312965-5312984 | MsG0180000386.01.T01:CDS | 40.0% | |
| AAGAGGTTGCATCAATTGTG+TGG | + | Chr1:5312810-5312829 | MsG0180000386.01.T01:CDS | 40.0% | |
| ATAGACTAGTGAAACTGCAG+CGG | + | Chr1:5312383-5312402 | MsG0180000386.01.T01:intron | 40.0% | |
| ATATTGCTCTTGAGTCACCA+AGG | - | Chr1:5311777-5311796 | None:intergenic | 40.0% | |
| CAAAGAAGTTTCCTTCCTCT+AGG | + | Chr1:5312894-5312913 | MsG0180000386.01.T01:CDS | 40.0% | |
| CGGTTGTGTTTATGCGTTAA+AGG | + | Chr1:5312509-5312528 | MsG0180000386.01.T01:CDS | 40.0% | |
| GCAGAAGAAACCACAAAACT+TGG | + | Chr1:5312949-5312968 | MsG0180000386.01.T01:CDS | 40.0% | |
| GGTGACTCAAGAGCAATATT+AGG | + | Chr1:5311778-5311797 | MsG0180000386.01.T01:intron | 40.0% | |
| TGCTGTAGTTGTCATAAGAC+AGG | + | Chr1:5311645-5311664 | MsG0180000386.01.T01:CDS | 40.0% | |
| TTAAGCTGCTGATGTCTTCA+AGG | + | Chr1:5312043-5312062 | MsG0180000386.01.T01:intron | 40.0% | |
| ! | ACTAGTCTATCGTGATGCTT+TGG | - | Chr1:5312373-5312392 | None:intergenic | 40.0% |
| !! | AGTTTTGTGGTTTCTTCTGC+AGG | - | Chr1:5312949-5312968 | None:intergenic | 40.0% |
| !! | ATTGACCACTGATTTGAAGC+CGG | + | Chr1:5311833-5311852 | MsG0180000386.01.T01:intron | 40.0% |
| AACCACATGTCCAACGAGTA+TGG | + | Chr1:5312535-5312554 | MsG0180000386.01.T01:CDS | 45.0% | |
| AACCATACTCGTTGGACATG+TGG | - | Chr1:5312540-5312559 | None:intergenic | 45.0% | |
| CAATCATCTACCCTAGAGGA+AGG | - | Chr1:5312908-5312927 | None:intergenic | 45.0% | |
| CAGGCGAAGCAAAGAGAATA+AGG | + | Chr1:5312478-5312497 | MsG0180000386.01.T01:CDS | 45.0% | |
| CTGGTGTAGCAATAACACCA+TGG | - | Chr1:5312626-5312645 | None:intergenic | 45.0% | |
| GGTTGCATCAATTGTGTGGA+TGG | + | Chr1:5312814-5312833 | MsG0180000386.01.T01:CDS | 45.0% | |
| GTGGTTTCTTCTGCAGGAAA+TGG | - | Chr1:5312943-5312962 | None:intergenic | 45.0% | |
| AGAGTGAAGAGGAAGCTGCA+AGG | + | Chr1:5312840-5312859 | MsG0180000386.01.T01:CDS | 50.0% | |
| AGGCCAGGGTAGTTCTCATT+GGG | - | Chr1:5312564-5312583 | None:intergenic | 50.0% | |
| ATCTCCGAAAGCTCGAGACA+TGG | - | Chr1:5312589-5312608 | None:intergenic | 50.0% | |
| CATTGGGCAACCATACTCGT+TGG | - | Chr1:5312548-5312567 | None:intergenic | 50.0% | |
| CTAGCCATGTCTCGAGCTTT+CGG | + | Chr1:5312582-5312601 | MsG0180000386.01.T01:CDS | 50.0% | |
| CTGCAGGAAATGGCAAACCA+CGG | - | Chr1:5312933-5312952 | None:intergenic | 50.0% | |
| GAGTGAAGAGGAAGCTGCAA+GGG | + | Chr1:5312841-5312860 | MsG0180000386.01.T01:CDS | 50.0% | |
| GCAATCCGGCTTCAAATCAG+TGG | - | Chr1:5311841-5311860 | None:intergenic | 50.0% | |
| GGTGCAATCATCTACCCTAG+AGG | - | Chr1:5312912-5312931 | None:intergenic | 50.0% | |
| GTGGATGGTAGAGAGTGAAG+AGG | + | Chr1:5312829-5312848 | MsG0180000386.01.T01:CDS | 50.0% | |
| TAGGCCAGGGTAGTTCTCAT+TGG | - | Chr1:5312565-5312584 | None:intergenic | 50.0% | |
| TAGGGTAGATGATTGCACCG+TGG | + | Chr1:5312913-5312932 | MsG0180000386.01.T01:CDS | 50.0% | |
| TTGCCCAATGAGAACTACCC+TGG | + | Chr1:5312558-5312577 | MsG0180000386.01.T01:CDS | 50.0% | |
| CGAAAGCTCGAGACATGGCT+AGG | - | Chr1:5312584-5312603 | None:intergenic | 55.0% | |
| CTTCTTTGCCCATGCAGCTG+TGG | - | Chr1:5312882-5312901 | None:intergenic | 55.0% | |
| AGAGGAAGCTGCAAGGGCAG+TGG | + | Chr1:5312847-5312866 | MsG0180000386.01.T01:CDS | 60.0% | |
| CTCGAGACATGGCTAGGCCA+GGG | - | Chr1:5312578-5312597 | None:intergenic | 60.0% | |
| GGAAGCAGCCACAGCTGCAT+GGG | + | Chr1:5312871-5312890 | MsG0180000386.01.T01:CDS | 60.0% | |
| TGGAAGCAGCCACAGCTGCA+TGG | + | Chr1:5312870-5312889 | MsG0180000386.01.T01:CDS | 60.0% | |
| GCTCGAGACATGGCTAGGCC+AGG | - | Chr1:5312579-5312598 | None:intergenic | 65.0% | |
| GGAAGCTGCAAGGGCAGTGG+TGG | + | Chr1:5312850-5312869 | MsG0180000386.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 5311589 | 5312994 | 5311589 | ID=MsG0180000386.01;Name=MsG0180000386.01 |
| Chr1 | mRNA | 5311589 | 5312994 | 5311589 | ID=MsG0180000386.01.T01;Parent=MsG0180000386.01;Name=MsG0180000386.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|1|1|3|0|200 |
| Chr1 | exon | 5311589 | 5311666 | 5311589 | ID=MsG0180000386.01.T01:exon:28268;Parent=MsG0180000386.01.T01 |
| Chr1 | exon | 5312387 | 5312704 | 5312387 | ID=MsG0180000386.01.T01:exon:28267;Parent=MsG0180000386.01.T01 |
| Chr1 | exon | 5312788 | 5312994 | 5312788 | ID=MsG0180000386.01.T01:exon:28266;Parent=MsG0180000386.01.T01 |
| Chr1 | CDS | 5311589 | 5311666 | 5311589 | ID=MsG0180000386.01.T01:cds;Parent=MsG0180000386.01.T01 |
| Chr1 | CDS | 5312387 | 5312704 | 5312387 | ID=MsG0180000386.01.T01:cds;Parent=MsG0180000386.01.T01 |
| Chr1 | CDS | 5312788 | 5312994 | 5312788 | ID=MsG0180000386.01.T01:cds;Parent=MsG0180000386.01.T01 |
| Gene Sequence |
| Protein sequence |
