AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000385.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000385.01.T01 MTR_1g016620 93.814 97 4 1 24 120 250 344 2.13e-44 147
MsG0180000385.01.T01 MTR_4g037470 56.410 78 31 2 31 107 298 373 1.34e-19 83.2
MsG0180000385.01.T01 MTR_4g037470 56.410 78 31 2 31 107 307 382 1.37e-19 83.2
MsG0180000385.01.T01 MTR_7g021530 50.000 80 37 2 30 109 246 322 2.02e-19 82.0
MsG0180000385.01.T01 MTR_7g021530 49.412 85 39 3 30 113 337 418 2.35e-19 82.4
MsG0180000385.01.T01 MTR_7g021530 49.412 85 39 3 30 113 296 377 2.35e-19 82.4
MsG0180000385.01.T01 MTR_7g080170 50.000 82 39 1 30 111 204 283 2.88e-19 82.0
MsG0180000385.01.T01 MTR_7g070510 45.570 79 41 1 30 108 292 368 3.40e-19 82.0
MsG0180000385.01.T01 MTR_7g080170 50.000 82 39 1 30 111 312 391 4.79e-19 81.6
MsG0180000385.01.T01 MTR_2g090190 57.692 78 30 2 31 107 291 366 5.86e-18 78.6
MsG0180000385.01.T01 MTR_8g017240 52.632 76 32 3 30 105 269 340 4.82e-17 75.5
MsG0180000385.01.T01 MTR_4g013295 43.373 83 45 1 30 112 271 351 1.41e-16 74.3
MsG0180000385.01.T01 MTR_4g120410 38.750 80 47 1 30 109 280 357 2.33e-13 65.1
MsG0180000385.01.T01 MTR_7g112490 43.662 71 38 1 35 105 312 380 1.85e-12 62.8
MsG0180000385.01.T01 MTR_7g112490 43.662 71 38 1 35 105 294 362 2.47e-12 62.4
MsG0180000385.01.T01 MTR_1g083690 43.056 72 39 1 34 105 282 351 7.58e-12 60.8
MsG0180000385.01.T01 MTR_2g435550 38.462 78 46 1 33 110 284 359 8.76e-12 60.8
MsG0180000385.01.T01 MTR_2g435550 38.462 78 46 1 33 110 270 345 9.42e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000385.01.T01 AT4G32950 60.759 79 27 2 33 109 247 323 3.37e-23 92.0
MsG0180000385.01.T01 AT5G36250 52.439 82 37 1 30 111 312 391 2.65e-22 90.5
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 217 290 2.90e-22 90.1
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 217 290 2.90e-22 90.1
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 217 290 2.90e-22 90.1
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 217 290 2.90e-22 90.1
MsG0180000385.01.T01 AT5G36250 47.872 94 37 1 30 111 312 405 3.41e-22 90.5
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 327 400 3.86e-22 90.5
MsG0180000385.01.T01 AT1G16220 57.895 76 30 1 30 105 304 377 3.90e-22 90.5
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 257 330 4.35e-22 90.1
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 257 330 4.35e-22 90.1
MsG0180000385.01.T01 AT1G79630 59.211 76 29 1 30 105 323 396 4.61e-22 90.1
MsG0180000385.01.T01 AT4G03415 57.692 78 30 2 31 107 300 375 1.39e-17 77.4
MsG0180000385.01.T01 AT4G03415 57.692 78 30 2 31 107 300 375 1.39e-17 77.4
MsG0180000385.01.T01 AT4G03415 57.692 78 30 2 31 107 300 375 1.39e-17 77.4
MsG0180000385.01.T01 AT1G03590 56.410 78 31 2 31 107 290 365 2.53e-17 76.6
MsG0180000385.01.T01 AT3G02750 50.617 81 38 1 30 110 321 399 2.98e-17 76.6
MsG0180000385.01.T01 AT3G02750 50.617 81 38 1 30 110 321 399 2.98e-17 76.6
MsG0180000385.01.T01 AT3G02750 50.617 81 38 1 30 110 356 434 3.52e-17 76.3
MsG0180000385.01.T01 AT5G01700 41.772 79 44 1 30 108 216 292 5.33e-16 72.4
MsG0180000385.01.T01 AT5G01700 41.772 79 44 1 30 108 265 341 5.43e-16 72.8
MsG0180000385.01.T01 AT5G26010 48.148 81 39 2 30 109 251 329 6.40e-14 66.6
MsG0180000385.01.T01 AT3G16800 38.158 76 45 1 30 105 276 349 5.70e-13 64.3
MsG0180000385.01.T01 AT3G16800 38.158 76 45 1 30 105 276 349 5.70e-13 64.3
MsG0180000385.01.T01 AT3G16800 38.158 76 45 1 30 105 276 349 5.70e-13 64.3
MsG0180000385.01.T01 AT3G16800 38.158 76 45 1 30 105 181 254 5.73e-13 63.5
MsG0180000385.01.T01 AT3G16800 38.158 76 45 1 30 105 181 254 5.73e-13 63.5
MsG0180000385.01.T01 AT5G27930 44.928 69 36 1 35 103 287 353 7.90e-12 60.8
MsG0180000385.01.T01 AT5G27930 44.928 69 36 1 35 103 287 353 7.90e-12 60.8

Find 36 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCACAAAACTTGGAGTATA+TGG 0.271089 1:-5306515 MsG0180000385.01.T01:CDS
GATAGTAGTCAAAGGGTATT+TGG 0.271723 1:-5306842 MsG0180000385.01.T01:intron
TATATGGACAGTGGAAAATT+AGG 0.305057 1:-5306499 MsG0180000385.01.T01:CDS
GTGGTTTCTTCTGCAGGAAA+TGG 0.397763 1:+5306533 None:intergenic
CACTTGCAAGAACAATAAAT+TGG 0.399587 1:+5306796 None:intergenic
ATTGGTCACTTGATGTTAAA+CGG 0.419251 1:+5306814 None:intergenic
ACTCGGCACATGAGTGAGTT+TGG 0.423881 1:-5307211 None:intergenic
TTTATTGTTCTTGCAAGTGA+TGG 0.439321 1:-5306792 MsG0180000385.01.T01:CDS
GCAGAAGAAACCACAAAACT+TGG 0.461758 1:-5306524 MsG0180000385.01.T01:CDS
CCTAGCCTCAACTTCTGTCA+TGG 0.462474 1:+5307144 None:intergenic
CTAGCCTCAACTTCTGTCAT+GGG 0.465993 1:+5307145 None:intergenic
CTTCTTTGCCCATGCAGCTG+TGG 0.475385 1:+5306594 None:intergenic
TTATTGTTCTTGCAAGTGAT+GGG 0.484730 1:-5306791 MsG0180000385.01.T01:CDS
GGAAGCAGCCACAGCTGCAT+GGG 0.485485 1:-5306602 MsG0180000385.01.T01:CDS
GGAAGCTGCAAGGGCAGTGG+TGG 0.495822 1:-5306623 MsG0180000385.01.T01:CDS
GAGTGAAGAGGAAGCTGCAA+GGG 0.496594 1:-5306632 MsG0180000385.01.T01:CDS
CAAAGAAGTTTCCTTCCTCT+AGG 0.507704 1:-5306579 MsG0180000385.01.T01:CDS
TGGAAGCAGCCACAGCTGCA+TGG 0.533129 1:-5306603 MsG0180000385.01.T01:CDS
AAAGAAGTTTCCTTCCTCTA+GGG 0.562358 1:-5306578 MsG0180000385.01.T01:CDS
AGAGGAAGCTGCAAGGGCAG+TGG 0.574488 1:-5306626 MsG0180000385.01.T01:CDS
GACAGAAGTTGAGGCTAGGA+CGG 0.576331 1:-5307140 MsG0180000385.01.T01:intron
TAGCCTCAACTTCTGTCATG+GGG 0.587508 1:+5307146 None:intergenic
CCATGACAGAAGTTGAGGCT+AGG 0.598676 1:-5307144 MsG0180000385.01.T01:CDS
CAATCATCTACCCTAGAGGA+AGG 0.600898 1:+5306568 None:intergenic
AGAGTGAAGAGGAAGCTGCA+AGG 0.613435 1:-5306633 MsG0180000385.01.T01:CDS
TATTGTTCTTGCAAGTGATG+GGG 0.615238 1:-5306790 MsG0180000385.01.T01:CDS
GGTTGCATCAATTGTGTGGA+TGG 0.622975 1:-5306659 MsG0180000385.01.T01:CDS
GTGGATGGTAGAGAGTGAAG+AGG 0.644994 1:-5306644 MsG0180000385.01.T01:CDS
AAGAGGTTGCATCAATTGTG+TGG 0.658363 1:-5306663 MsG0180000385.01.T01:CDS
AAAATACAAATAGAGAGTGG+GGG 0.671672 1:-5307178 MsG0180000385.01.T01:CDS
AACTTGGAGTATATGGACAG+TGG 0.675658 1:-5306508 MsG0180000385.01.T01:CDS
GGATGTACTAAGTAATGAAG+AGG 0.676441 1:-5306680 MsG0180000385.01.T01:CDS
CTGCAGGAAATGGCAAACCA+CGG 0.691531 1:+5306543 None:intergenic
AGCCTCAACTTCTGTCATGG+GGG 0.697685 1:+5307147 None:intergenic
GGTGCAATCATCTACCCTAG+AGG 0.703711 1:+5306564 None:intergenic
TAGGGTAGATGATTGCACCG+TGG 0.761662 1:-5306560 MsG0180000385.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATGATAGTAGTCAA+AGG - Chr1:5306847-5306866 MsG0180000385.01.T01:intron 20.0%
!! TATTTATGATAGTAGTCAAA+GGG - Chr1:5306848-5306867 MsG0180000385.01.T01:intron 20.0%
!!! AATGGCTTTATCATTATTTA+TGG - Chr1:5306972-5306991 MsG0180000385.01.T01:intron 20.0%
!!! TATGGATATTTTTTGCTTAT+AGG - Chr1:5306990-5307009 MsG0180000385.01.T01:intron 20.0%
! AAAAATACAAATAGAGAGTG+GGG - Chr1:5306518-5306537 MsG0180000385.01.T01:CDS 25.0%
! CAAAAATACAAATAGAGAGT+GGG - Chr1:5306517-5306536 MsG0180000385.01.T01:CDS 25.0%
! GACATAACATACAAATAAAG+TGG - Chr1:5306763-5306782 MsG0180000385.01.T01:intron 25.0%
! TAAATTACTCTGATTGAACA+TGG + Chr1:5306828-5306847 None:intergenic 25.0%
!!! ATATTTTTTGCTTATAGGTG+TGG - Chr1:5306995-5307014 MsG0180000385.01.T01:intron 25.0%
!!! TATTTTTTGCTTATAGGTGT+GGG - Chr1:5306996-5307015 MsG0180000385.01.T01:intron 25.0%
AAAATACAAATAGAGAGTGG+GGG - Chr1:5306519-5306538 MsG0180000385.01.T01:CDS 30.0%
ATTGGTCACTTGATGTTAAA+CGG + Chr1:5306886-5306905 None:intergenic 30.0%
CAAAACTGAAACAAGAGATA+AGG - Chr1:5306611-5306630 MsG0180000385.01.T01:CDS 30.0%
CACTTGCAAGAACAATAAAT+TGG + Chr1:5306904-5306923 None:intergenic 30.0%
CCAAAAATACAAATAGAGAG+TGG - Chr1:5306516-5306535 MsG0180000385.01.T01:CDS 30.0%
CTTGCATACAACAATTTCAA+TGG - Chr1:5306954-5306973 MsG0180000385.01.T01:intron 30.0%
TATATGGACAGTGGAAAATT+AGG - Chr1:5307198-5307217 MsG0180000385.01.T01:CDS 30.0%
TTATTGTTCTTGCAAGTGAT+GGG - Chr1:5306906-5306925 MsG0180000385.01.T01:intron 30.0%
TTTATTGTTCTTGCAAGTGA+TGG - Chr1:5306905-5306924 MsG0180000385.01.T01:intron 30.0%
! CCACTCTCTATTTGTATTTT+TGG + Chr1:5306519-5306538 None:intergenic 30.0%
!! CTTATCTCTTGTTTCAGTTT+TGG + Chr1:5306613-5306632 None:intergenic 30.0%
!!! CTCTCTATTTGTATTTTTGG+CGG + Chr1:5306516-5306535 None:intergenic 30.0%
AAAGAAGTTTCCTTCCTCTA+GGG - Chr1:5307119-5307138 MsG0180000385.01.T01:intron 35.0%
AAATGCATAGCCTTTCTTTG+TGG - Chr1:5306652-5306671 MsG0180000385.01.T01:CDS 35.0%
AATGCATAGCCTTTCTTTGT+GGG - Chr1:5306653-5306672 MsG0180000385.01.T01:CDS 35.0%
ACCACAAAACTTGGAGTATA+TGG - Chr1:5307182-5307201 MsG0180000385.01.T01:CDS 35.0%
GGATGTACTAAGTAATGAAG+AGG - Chr1:5307017-5307036 MsG0180000385.01.T01:intron 35.0%
TATTGTTCTTGCAAGTGATG+GGG - Chr1:5306907-5306926 MsG0180000385.01.T01:intron 35.0%
TTACTCTGATTGAACATGGA+TGG + Chr1:5306824-5306843 None:intergenic 35.0%
! TCCATATACTCCAAGTTTTG+TGG + Chr1:5307186-5307205 None:intergenic 35.0%
!! GATAGTAGTCAAAGGGTATT+TGG - Chr1:5306855-5306874 MsG0180000385.01.T01:intron 35.0%
AAATGTTGCGGACAAGAGTT+TGG + Chr1:5306796-5306815 None:intergenic 40.0%
AACTTGGAGTATATGGACAG+TGG - Chr1:5307189-5307208 MsG0180000385.01.T01:CDS 40.0%
AAGAGGTTGCATCAATTGTG+TGG - Chr1:5307034-5307053 MsG0180000385.01.T01:intron 40.0%
CAAAGAAGTTTCCTTCCTCT+AGG - Chr1:5307118-5307137 MsG0180000385.01.T01:intron 40.0%
GCAGAAGAAACCACAAAACT+TGG - Chr1:5307173-5307192 MsG0180000385.01.T01:CDS 40.0%
!! AGTTTTGTGGTTTCTTCTGC+AGG + Chr1:5307173-5307192 None:intergenic 40.0%
CAATCATCTACCCTAGAGGA+AGG + Chr1:5307132-5307151 None:intergenic 45.0%
CTAGCCTCAACTTCTGTCAT+GGG + Chr1:5306555-5306574 None:intergenic 45.0%
GACGGTTAGCAGGGATAAAA+GGG - Chr1:5306575-5306594 MsG0180000385.01.T01:CDS 45.0%
GGTTGCATCAATTGTGTGGA+TGG - Chr1:5307038-5307057 MsG0180000385.01.T01:intron 45.0%
GTGGTTTCTTCTGCAGGAAA+TGG + Chr1:5307167-5307186 None:intergenic 45.0%
TAGCCTCAACTTCTGTCATG+GGG + Chr1:5306554-5306573 None:intergenic 45.0%
TCGTGTTGTCCCACAAAGAA+AGG + Chr1:5306665-5306684 None:intergenic 45.0%
TGGATGGTGCAGAAATGTTG+CGG + Chr1:5306808-5306827 None:intergenic 45.0%
AGAGTGAAGAGGAAGCTGCA+AGG - Chr1:5307064-5307083 MsG0180000385.01.T01:intron 50.0%
AGCCTCAACTTCTGTCATGG+GGG + Chr1:5306553-5306572 None:intergenic 50.0%
CCATGACAGAAGTTGAGGCT+AGG - Chr1:5306553-5306572 MsG0180000385.01.T01:CDS 50.0%
CCTAGCCTCAACTTCTGTCA+TGG + Chr1:5306556-5306575 None:intergenic 50.0%
CTGCAGGAAATGGCAAACCA+CGG + Chr1:5307157-5307176 None:intergenic 50.0%
GACAGAAGTTGAGGCTAGGA+CGG - Chr1:5306557-5306576 MsG0180000385.01.T01:CDS 50.0%
GAGTGAAGAGGAAGCTGCAA+GGG - Chr1:5307065-5307084 MsG0180000385.01.T01:intron 50.0%
GGACGGTTAGCAGGGATAAA+AGG - Chr1:5306574-5306593 MsG0180000385.01.T01:CDS 50.0%
GGTGCAATCATCTACCCTAG+AGG + Chr1:5307136-5307155 None:intergenic 50.0%
GTGGATGGTAGAGAGTGAAG+AGG - Chr1:5307053-5307072 MsG0180000385.01.T01:intron 50.0%
TAGGGTAGATGATTGCACCG+TGG - Chr1:5307137-5307156 MsG0180000385.01.T01:intron 50.0%
TTGTGGGACAACACGAGACA+GGG - Chr1:5306669-5306688 MsG0180000385.01.T01:CDS 50.0%
TTTGTGGGACAACACGAGAC+AGG - Chr1:5306668-5306687 MsG0180000385.01.T01:CDS 50.0%
CTTCTTTGCCCATGCAGCTG+TGG + Chr1:5307106-5307125 None:intergenic 55.0%
TGAGGCTAGGACGGTTAGCA+GGG - Chr1:5306566-5306585 MsG0180000385.01.T01:CDS 55.0%
TTGAGGCTAGGACGGTTAGC+AGG - Chr1:5306565-5306584 MsG0180000385.01.T01:CDS 55.0%
AGAGGAAGCTGCAAGGGCAG+TGG - Chr1:5307071-5307090 MsG0180000385.01.T01:intron 60.0%
CGCCCCCATGACAGAAGTTG+AGG - Chr1:5306548-5306567 MsG0180000385.01.T01:CDS 60.0%
GGAAGCAGCCACAGCTGCAT+GGG - Chr1:5307095-5307114 MsG0180000385.01.T01:intron 60.0%
GGGACAACACGAGACAGGGT+AGG - Chr1:5306673-5306692 MsG0180000385.01.T01:CDS 60.0%
TGGAAGCAGCCACAGCTGCA+TGG - Chr1:5307094-5307113 MsG0180000385.01.T01:intron 60.0%
GGAAGCTGCAAGGGCAGTGG+TGG - Chr1:5307074-5307093 MsG0180000385.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5306495 5307224 5306495 ID=MsG0180000385.01;Name=MsG0180000385.01
Chr1 mRNA 5306495 5307224 5306495 ID=MsG0180000385.01.T01;Parent=MsG0180000385.01;Name=MsG0180000385.01.T01;_AED=0.50;_eAED=0.67;_QI=0|0|0|1|0|0.66|3|0|120
Chr1 exon 5307141 5307224 5307141 ID=MsG0180000385.01.T01:exon:2753;Parent=MsG0180000385.01.T01
Chr1 exon 5306785 5306850 5306785 ID=MsG0180000385.01.T01:exon:2754;Parent=MsG0180000385.01.T01
Chr1 exon 5306495 5306707 5306495 ID=MsG0180000385.01.T01:exon:2755;Parent=MsG0180000385.01.T01
Chr1 CDS 5307141 5307224 5307141 ID=MsG0180000385.01.T01:cds;Parent=MsG0180000385.01.T01
Chr1 CDS 5306785 5306850 5306785 ID=MsG0180000385.01.T01:cds;Parent=MsG0180000385.01.T01
Chr1 CDS 5306495 5306707 5306495 ID=MsG0180000385.01.T01:cds;Parent=MsG0180000385.01.T01
Gene Sequence

>MsG0180000385.01.T01

ATGAGTGAGTTTGGAATACCGCCAAAAATACAAATAGAGAGTGGGGGAAGTTACGCCCCCATGACAGAAGTTGAGGCTAGGACGGGTATTTGGTATCACCGTTTAACATCAAGTGACCAATTTATTGTTCTTGCAAGTGATGGGGTACGTGTGTGGGATGTACTAAGTAATGAAGAGGTTGCATCAATTGTGTGGATGGTAGAGAGTGAAGAGGAAGCTGCAAGGGCAGTGGTGGAAGCAGCCACAGCTGCATGGGCAAAGAAGTTTCCTTCCTCTAGGGTAGATGATTGCACCGTGGTTTGCCATTTCCTGCAGAAGAAACCACAAAACTTGGAGTATATGGACAGTGGAAAATTAGGTTAA

Protein sequence

>MsG0180000385.01.T01

MSEFGIPPKIQIESGGSYAPMTEVEARTGIWYHRLTSSDQFIVLASDGVRVWDVLSNEEVASIVWMVESEEEAARAVVEAATAAWAKKFPSSRVDDCTVVCHFLQKKPQNLEYMDSGKLG*