AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005270.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005270.01.T01 MTR_1g095950 98.361 122 2 0 1 122 16 137 4.12e-70 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCGGGCCACGGTCCAAAT+TGG 0.259697 1:-89409031 MsG0180005270.01.T01:CDS
TCTGGCTTTGGTAGAGGTTA+TGG 0.264164 1:-89408936 MsG0180005270.01.T01:CDS
AGTGGATGGGGTTACGGTTC+AGG 0.271763 1:-89408975 MsG0180005270.01.T01:CDS
AGAGGTTATGGCTATGGTTT+TGG 0.272187 1:-89408924 MsG0180005270.01.T01:CDS
GGTTCAGGACATTCTCCTTC+TGG 0.275589 1:-89408954 MsG0180005270.01.T01:CDS
GGAAGTGGTTCTGGCAATTC+AGG 0.279092 1:-89408831 MsG0180005270.01.T01:CDS
GGTGCTCATGGTGGTGGTTA+TGG 0.280938 1:-89408852 MsG0180005270.01.T01:CDS
GGTGGTTATGGAAGTGGTTC+TGG 0.286337 1:-89408840 MsG0180005270.01.T01:CDS
TGGGGTTACGGTTCAGGTTC+AGG 0.287425 1:-89408969 MsG0180005270.01.T01:CDS
CTCCGAAGGTGACCGATTAT+TGG 0.290144 1:+89408782 None:intergenic
TCTGGATCCGGGTCTGGTTA+TGG 0.298117 1:-89408888 MsG0180005270.01.T01:CDS
ACCGGGTCTGGGTCTGGATC+CGG 0.302126 1:-89408900 MsG0180005270.01.T01:CDS
GCCACTGCTGGCTGCGGCCA+TGG 0.313937 1:+89409103 None:intergenic
CCCCAGCTATAGTCCCAATT+TGG 0.314443 1:+89409017 None:intergenic
TTTGGAACCGGGTCTGGGTC+TGG 0.316778 1:-89408906 MsG0180005270.01.T01:CDS
TCCGACAGGTGCAAGTGGTT+CGG 0.329780 1:-89409048 MsG0180005270.01.T01:CDS
GGACATTCTCCTTCTGGCTT+TGG 0.332030 1:-89408948 MsG0180005270.01.T01:CDS
CCGACAGGTGCAAGTGGTTC+GGG 0.360896 1:-89409047 MsG0180005270.01.T01:CDS
TCGGTCACCTTCGGAGTCTA+AGG 0.368892 1:-89408775 MsG0180005270.01.T01:CDS
GTCTGCACCAGGAAGTGGAT+GGG 0.371552 1:-89408988 MsG0180005270.01.T01:CDS
TCCGGGTCTGGTTATGGATA+TGG 0.372940 1:-89408882 MsG0180005270.01.T01:CDS
CCGTAACCCCATCCACTTCC+TGG 0.374994 1:+89408981 None:intergenic
GGTCTGCACCAGGAAGTGGA+TGG 0.384555 1:-89408989 MsG0180005270.01.T01:CDS
CCGGGTCTGGGTCTGGATCC+GGG 0.384946 1:-89408899 MsG0180005270.01.T01:CDS
TCTGGCAATTCAGGCGGTGG+AGG 0.390126 1:-89408822 MsG0180005270.01.T01:CDS
TGGGACTATAGCTGGGGATG+GGG 0.391784 1:-89409011 MsG0180005270.01.T01:CDS
TAGCTTTCTTCCGCCACTGC+TGG 0.402255 1:+89409091 None:intergenic
TCTGGGTCTGGATCCGGGTC+TGG 0.404022 1:-89408894 MsG0180005270.01.T01:CDS
TTCGGGCCACGGTCCAAATT+GGG 0.409857 1:-89409030 MsG0180005270.01.T01:CDS
TCTCCTTCTGGCTTTGGTAG+AGG 0.416778 1:-89408942 MsG0180005270.01.T01:CDS
CCAGGAAGTGGATGGGGTTA+CGG 0.422757 1:-89408981 MsG0180005270.01.T01:CDS
TTTGGTAGAGGTTATGGCTA+TGG 0.433764 1:-89408930 MsG0180005270.01.T01:CDS
GTCCAAATTGGGACTATAGC+TGG 0.434287 1:-89409019 MsG0180005270.01.T01:CDS
GGGACTATAGCTGGGGATGG+GGG 0.436504 1:-89409010 MsG0180005270.01.T01:CDS
GGCGGTGGAGGGCATGGTGG+TGG 0.444613 1:-89408810 MsG0180005270.01.T01:CDS
AATTCAGGCGGTGGAGGGCA+TGG 0.452407 1:-89408816 MsG0180005270.01.T01:CDS
TTTAATTACTCATGGTTGGT+TGG 0.458788 1:+89408746 None:intergenic
TCTTCCGCCACTGCTGGCTG+CGG 0.464894 1:+89409097 None:intergenic
TATGGTAGTGGTGGTGCTCA+TGG 0.465660 1:-89408864 MsG0180005270.01.T01:CDS
GGTGCAAGTGGTTCGGGCCA+CGG 0.474288 1:-89409041 MsG0180005270.01.T01:CDS
AGTGGTGGTGCTCATGGTGG+TGG 0.480579 1:-89408858 MsG0180005270.01.T01:CDS
TCCAAATTGGGACTATAGCT+GGG 0.483029 1:-89409018 MsG0180005270.01.T01:CDS
AACCAATAATCGGTCACCTT+CGG 0.485230 1:-89408784 MsG0180005270.01.T01:CDS
TTAATTTAATTACTCATGGT+TGG 0.488357 1:+89408742 None:intergenic
TTGGGACTATAGCTGGGGAT+GGG 0.492750 1:-89409012 MsG0180005270.01.T01:CDS
CTGGCAATTCAGGCGGTGGA+GGG 0.495997 1:-89408821 MsG0180005270.01.T01:CDS
TCCATGGCCGCAGCCAGCAG+TGG 0.511233 1:-89409104 MsG0180005270.01.T01:CDS
CATGGTGGTGGTTATGGAAG+TGG 0.512562 1:-89408846 MsG0180005270.01.T01:CDS
ATGGCCGCAGCCAGCAGTGG+CGG 0.520646 1:-89409101 MsG0180005270.01.T01:CDS
GCAAGCGGTGGTTCTCCGAC+AGG 0.530173 1:-89409062 MsG0180005270.01.T01:CDS
GTGGTGGTAGAACCAATAAT+CGG 0.540663 1:-89408794 MsG0180005270.01.T01:CDS
GGTTCTGGCAATTCAGGCGG+TGG 0.551946 1:-89408825 MsG0180005270.01.T01:CDS
TCTGCACCAGGAAGTGGATG+GGG 0.554469 1:-89408987 MsG0180005270.01.T01:CDS
CGGTCACCTTCGGAGTCTAA+GGG 0.558343 1:-89408774 MsG0180005270.01.T01:CDS
CCCGGATCCAGACCCAGACC+CGG 0.567752 1:+89408899 None:intergenic
ATTGGGACTATAGCTGGGGA+TGG 0.573906 1:-89409013 MsG0180005270.01.T01:CDS
TCTGGTTATGGATATGGTAG+TGG 0.576517 1:-89408876 MsG0180005270.01.T01:CDS
CCCGAACCACTTGCACCTGT+CGG 0.592937 1:+89409047 None:intergenic
TCAGGCGGTGGAGGGCATGG+TGG 0.594015 1:-89408813 MsG0180005270.01.T01:CDS
AAGCTAGACACGGCAAGCGG+TGG 0.607144 1:-89409074 MsG0180005270.01.T01:CDS
GGTTATGGATATGGTAGTGG+TGG 0.613803 1:-89408873 MsG0180005270.01.T01:CDS
GGTAGTGGTGGTGCTCATGG+TGG 0.614244 1:-89408861 MsG0180005270.01.T01:CDS
GGTTCTCCGACAGGTGCAAG+TGG 0.627044 1:-89409053 MsG0180005270.01.T01:CDS
ACCATATCCATAACCAGACC+CGG 0.634754 1:+89408881 None:intergenic
CCAAATTGGGACTATAGCTG+GGG 0.653928 1:-89409017 MsG0180005270.01.T01:CDS
TAACCTCTACCAAAGCCAGA+AGG 0.659441 1:+89408939 None:intergenic
AGTGGTTCTGGCAATTCAGG+CGG 0.663940 1:-89408828 MsG0180005270.01.T01:CDS
TATAGTCCCAATTTGGACCG+TGG 0.667912 1:+89409024 None:intergenic
AGAAAGCTAGACACGGCAAG+CGG 0.701647 1:-89409077 MsG0180005270.01.T01:CDS
TGGCGGAAGAAAGCTAGACA+CGG 0.719665 1:-89409084 MsG0180005270.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACCAATAATCGGTCACCTT+CGG - Chr1:89409072-89409091 MsG0180005270.01.T01:CDS 40.0%
GTGGTGGTAGAACCAATAAT+CGG - Chr1:89409062-89409081 MsG0180005270.01.T01:CDS 40.0%
TCCAAATTGGGACTATAGCT+GGG - Chr1:89408838-89408857 MsG0180005270.01.T01:CDS 40.0%
TCTGGTTATGGATATGGTAG+TGG - Chr1:89408980-89408999 MsG0180005270.01.T01:CDS 40.0%
TTTGGTAGAGGTTATGGCTA+TGG - Chr1:89408926-89408945 MsG0180005270.01.T01:CDS 40.0%
! AGAGGTTATGGCTATGGTTT+TGG - Chr1:89408932-89408951 MsG0180005270.01.T01:CDS 40.0%
!!! TATGGCTATGGTTTTGGAAC+CGG - Chr1:89408938-89408957 MsG0180005270.01.T01:CDS 40.0%
ACCATATCCATAACCAGACC+CGG + Chr1:89408978-89408997 None:intergenic 45.0%
CCAAATTGGGACTATAGCTG+GGG - Chr1:89408839-89408858 MsG0180005270.01.T01:CDS 45.0%
GTCCAAATTGGGACTATAGC+TGG - Chr1:89408837-89408856 MsG0180005270.01.T01:CDS 45.0%
TAACCTCTACCAAAGCCAGA+AGG + Chr1:89408920-89408939 None:intergenic 45.0%
TATAGTCCCAATTTGGACCG+TGG + Chr1:89408835-89408854 None:intergenic 45.0%
! GTTTTACCCTTAGACTCCGA+AGG + Chr1:89409091-89409110 None:intergenic 45.0%
!! GGTTATGGATATGGTAGTGG+TGG - Chr1:89408983-89409002 MsG0180005270.01.T01:CDS 45.0%
!! TCTGGCTTTGGTAGAGGTTA+TGG - Chr1:89408920-89408939 MsG0180005270.01.T01:CDS 45.0%
!!! ATGGCTATGGTTTTGGAACC+GGG - Chr1:89408939-89408958 MsG0180005270.01.T01:CDS 45.0%
AGAAAGCTAGACACGGCAAG+CGG - Chr1:89408779-89408798 MsG0180005270.01.T01:CDS 50.0%
ATTGGGACTATAGCTGGGGA+TGG - Chr1:89408843-89408862 MsG0180005270.01.T01:CDS 50.0%
CCCCAGCTATAGTCCCAATT+TGG + Chr1:89408842-89408861 None:intergenic 50.0%
CTCCGAAGGTGACCGATTAT+TGG + Chr1:89409077-89409096 None:intergenic 50.0%
GGTGGTTATGGAAGTGGTTC+TGG - Chr1:89409016-89409035 MsG0180005270.01.T01:CDS 50.0%
GGTTCAGGACATTCTCCTTC+TGG - Chr1:89408902-89408921 MsG0180005270.01.T01:CDS 50.0%
TCCGGGTCTGGTTATGGATA+TGG - Chr1:89408974-89408993 MsG0180005270.01.T01:CDS 50.0%
TGGCGGAAGAAAGCTAGACA+CGG - Chr1:89408772-89408791 MsG0180005270.01.T01:CDS 50.0%
TTGGGACTATAGCTGGGGAT+GGG - Chr1:89408844-89408863 MsG0180005270.01.T01:CDS 50.0%
!! AGTGGTTCTGGCAATTCAGG+CGG - Chr1:89409028-89409047 MsG0180005270.01.T01:CDS 50.0%
!! CATGGTGGTGGTTATGGAAG+TGG - Chr1:89409010-89409029 MsG0180005270.01.T01:CDS 50.0%
!! GGAAGTGGTTCTGGCAATTC+AGG - Chr1:89409025-89409044 MsG0180005270.01.T01:CDS 50.0%
!! GGACATTCTCCTTCTGGCTT+TGG - Chr1:89408908-89408927 MsG0180005270.01.T01:CDS 50.0%
!! TATGGTAGTGGTGGTGCTCA+TGG - Chr1:89408992-89409011 MsG0180005270.01.T01:CDS 50.0%
!! TCTCCTTCTGGCTTTGGTAG+AGG - Chr1:89408914-89408933 MsG0180005270.01.T01:CDS 50.0%
!!! ATGGTTTTGGAACCGGGTCT+GGG - Chr1:89408945-89408964 MsG0180005270.01.T01:CDS 50.0%
!!! TATGGTTTTGGAACCGGGTC+TGG - Chr1:89408944-89408963 MsG0180005270.01.T01:CDS 50.0%
AGTGGATGGGGTTACGGTTC+AGG - Chr1:89408881-89408900 MsG0180005270.01.T01:CDS 55.0%
CCAGGAAGTGGATGGGGTTA+CGG - Chr1:89408875-89408894 MsG0180005270.01.T01:CDS 55.0%
CGGTCACCTTCGGAGTCTAA+GGG - Chr1:89409082-89409101 MsG0180005270.01.T01:CDS 55.0%
GTCTGCACCAGGAAGTGGAT+GGG - Chr1:89408868-89408887 MsG0180005270.01.T01:CDS 55.0%
TAGCTTTCTTCCGCCACTGC+TGG + Chr1:89408768-89408787 None:intergenic 55.0%
TCCGACAGGTGCAAGTGGTT+CGG - Chr1:89408808-89408827 MsG0180005270.01.T01:CDS 55.0%
TCGGTCACCTTCGGAGTCTA+AGG - Chr1:89409081-89409100 MsG0180005270.01.T01:CDS 55.0%
TCTGCACCAGGAAGTGGATG+GGG - Chr1:89408869-89408888 MsG0180005270.01.T01:CDS 55.0%
TCTGGATCCGGGTCTGGTTA+TGG - Chr1:89408968-89408987 MsG0180005270.01.T01:CDS 55.0%
TGGGACTATAGCTGGGGATG+GGG - Chr1:89408845-89408864 MsG0180005270.01.T01:CDS 55.0%
TTCGGGCCACGGTCCAAATT+GGG - Chr1:89408826-89408845 MsG0180005270.01.T01:CDS 55.0%
! TGGGGTTACGGTTCAGGTTC+AGG - Chr1:89408887-89408906 MsG0180005270.01.T01:CDS 55.0%
!! GGTGCTCATGGTGGTGGTTA+TGG - Chr1:89409004-89409023 MsG0180005270.01.T01:CDS 55.0%
AAGCTAGACACGGCAAGCGG+TGG - Chr1:89408782-89408801 MsG0180005270.01.T01:CDS 60.0%
AATTCAGGCGGTGGAGGGCA+TGG - Chr1:89409040-89409059 MsG0180005270.01.T01:CDS 60.0%
CCCGAACCACTTGCACCTGT+CGG + Chr1:89408812-89408831 None:intergenic 60.0%
CCGACAGGTGCAAGTGGTTC+GGG - Chr1:89408809-89408828 MsG0180005270.01.T01:CDS 60.0%
CCGTAACCCCATCCACTTCC+TGG + Chr1:89408878-89408897 None:intergenic 60.0%
CTGGCAATTCAGGCGGTGGA+GGG - Chr1:89409035-89409054 MsG0180005270.01.T01:CDS 60.0%
GGGACTATAGCTGGGGATGG+GGG - Chr1:89408846-89408865 MsG0180005270.01.T01:CDS 60.0%
GGTCTGCACCAGGAAGTGGA+TGG - Chr1:89408867-89408886 MsG0180005270.01.T01:CDS 60.0%
GGTTCTCCGACAGGTGCAAG+TGG - Chr1:89408803-89408822 MsG0180005270.01.T01:CDS 60.0%
GTTCGGGCCACGGTCCAAAT+TGG - Chr1:89408825-89408844 MsG0180005270.01.T01:CDS 60.0%
TCTGGCAATTCAGGCGGTGG+AGG - Chr1:89409034-89409053 MsG0180005270.01.T01:CDS 60.0%
! AGTGGTGGTGCTCATGGTGG+TGG - Chr1:89408998-89409017 MsG0180005270.01.T01:CDS 60.0%
! GGTAGTGGTGGTGCTCATGG+TGG - Chr1:89408995-89409014 MsG0180005270.01.T01:CDS 60.0%
! GGTTCTGGCAATTCAGGCGG+TGG - Chr1:89409031-89409050 MsG0180005270.01.T01:CDS 60.0%
! TTTGGAACCGGGTCTGGGTC+TGG - Chr1:89408950-89408969 MsG0180005270.01.T01:CDS 60.0%
ACCGGGTCTGGGTCTGGATC+CGG - Chr1:89408956-89408975 MsG0180005270.01.T01:CDS 65.0%
GCAAGCGGTGGTTCTCCGAC+AGG - Chr1:89408794-89408813 MsG0180005270.01.T01:CDS 65.0%
TCTGGGTCTGGATCCGGGTC+TGG - Chr1:89408962-89408981 MsG0180005270.01.T01:CDS 65.0%
TGGGGGTCTGCACCAGGAAG+TGG - Chr1:89408863-89408882 MsG0180005270.01.T01:CDS 65.0%
! GGTGCAAGTGGTTCGGGCCA+CGG - Chr1:89408815-89408834 MsG0180005270.01.T01:CDS 65.0%
! TCTTCCGCCACTGCTGGCTG+CGG + Chr1:89408762-89408781 None:intergenic 65.0%
ATGGCCGCAGCCAGCAGTGG+CGG - Chr1:89408755-89408774 MsG0180005270.01.T01:CDS 70.0%
CCCGGATCCAGACCCAGACC+CGG + Chr1:89408960-89408979 None:intergenic 70.0%
CCGGGTCTGGGTCTGGATCC+GGG - Chr1:89408957-89408976 MsG0180005270.01.T01:CDS 70.0%
TGGGGATGGGGGTCTGCACC+AGG - Chr1:89408857-89408876 MsG0180005270.01.T01:CDS 70.0%
! TCAGGCGGTGGAGGGCATGG+TGG - Chr1:89409043-89409062 MsG0180005270.01.T01:CDS 70.0%
! GGCGGTGGAGGGCATGGTGG+TGG - Chr1:89409046-89409065 MsG0180005270.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89408755 89409123 89408755 ID=MsG0180005270.01;Name=MsG0180005270.01
Chr1 mRNA 89408755 89409123 89408755 ID=MsG0180005270.01.T01;Parent=MsG0180005270.01;Name=MsG0180005270.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|122
Chr1 exon 89408755 89409123 89408755 ID=MsG0180005270.01.T01:exon:47744;Parent=MsG0180005270.01.T01
Chr1 CDS 89408755 89409123 89408755 ID=MsG0180005270.01.T01:cds;Parent=MsG0180005270.01.T01
Gene Sequence

>MsG0180005270.01.T01

ATGGCCGCAGCCAGCAGTGGCGGAAGAAAGCTAGACACGGCAAGCGGTGGTTCTCCGACAGGTGCAAGTGGTTCGGGCCACGGTCCAAATTGGGACTATAGCTGGGGATGGGGGTCTGCACCAGGAAGTGGATGGGGTTACGGTTCAGGTTCAGGACATTCTCCTTCTGGCTTTGGTAGAGGTTATGGCTATGGTTTTGGAACCGGGTCTGGGTCTGGATCCGGGTCTGGTTATGGATATGGTAGTGGTGGTGCTCATGGTGGTGGTTATGGAAGTGGTTCTGGCAATTCAGGCGGTGGAGGGCATGGTGGTGGTAGAACCAATAATCGGTCACCTTCGGAGTCTAAGGGTAAAACCAACCAACCATGA

Protein sequence

>MsG0180005270.01.T01

MAAASSGGRKLDTASGGSPTGASGSGHGPNWDYSWGWGSAPGSGWGYGSGSGHSPSGFGRGYGYGFGTGSGSGSGSGYGYGSGGAHGGGYGSGSGNSGGGGHGGGRTNNRSPSESKGKTNQP*