Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038636.01.T01 | RHN46009.1 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 7.87E-73 | 222 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038636.01.T01 | A0A396GY13 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 3.76e-73 | 222 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047944.01 | MsG0780038636.01 | 0.808208 | 3.467636e-50 | 1.970846e-47 |
| MsG0080047957.01 | MsG0780038636.01 | 0.808246 | 3.403028e-50 | 1.936083e-47 |
| MsG0080047959.01 | MsG0780038636.01 | 0.811300 | 7.499215e-51 | 4.625015e-48 |
| MsG0080048322.01 | MsG0780038636.01 | 0.801630 | 8.224343e-49 | 3.946601e-46 |
| MsG0080048462.01 | MsG0780038636.01 | 0.801497 | 8.756312e-49 | 4.188065e-46 |
| MsG0080049050.01 | MsG0780038636.01 | 0.807673 | 4.507235e-50 | 2.525773e-47 |
| MsG0180000114.01 | MsG0780038636.01 | 0.816776 | 4.645089e-52 | 3.322038e-49 |
| MsG0180000271.01 | MsG0780038636.01 | 0.828203 | 1.024607e-54 | 1.011481e-51 |
| MsG0180000284.01 | MsG0780038636.01 | 0.858117 | 1.026462e-62 | 2.591726e-59 |
| MsG0180000475.01 | MsG0780038636.01 | 0.822475 | 2.321778e-53 | 1.945453e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038636.01.T01 | MTR_7g058970 | 97.297 | 111 | 3 | 0 | 1 | 111 | 11 | 121 | 9.82e-77 | 222 |
| MsG0780038636.01.T01 | MTR_5g088550 | 63.636 | 110 | 31 | 4 | 1 | 109 | 1 | 102 | 6.34e-42 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038636.01.T01 | AT5G35732 | 42.982 | 114 | 48 | 5 | 1 | 111 | 1 | 100 | 3.87e-23 | 86.3 |
| MsG0780038636.01.T01 | AT2G04795 | 36.937 | 111 | 54 | 2 | 1 | 111 | 1 | 95 | 3.16e-19 | 76.3 |
| MsG0780038636.01.T01 | AT5G35732 | 37.736 | 106 | 49 | 5 | 1 | 103 | 1 | 92 | 8.53e-16 | 67.4 |
Find 28 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGGAAGAAGACAACAT+GGG | 0.729913 | 7:-50243051 | MsG0780038636.01.T01:CDS |
| AACATGTTTGAAGAAGGGAA+CGG | 0.661073 | 7:-50243103 | MsG0780038636.01.T01:CDS |
| AATTTGGCCAAATTCACCAG+TGG | 0.678766 | 7:+50242922 | None:intergenic |
| ACACAAGAGAGTATTGAAAT+TGG | 0.270980 | 7:+50242983 | None:intergenic |
| ACATGTTTGAAGAAGGGAAC+GGG | 0.488785 | 7:-50243102 | MsG0780038636.01.T01:CDS |
| AGGGTTGAAGTAGCAATGTT+TGG | 0.404594 | 7:-50243079 | MsG0780038636.01.T01:CDS |
| ATGAGAACATGTTTGAAGAA+GGG | 0.580253 | 7:-50243108 | MsG0780038636.01.T01:CDS |
| CAACATGGGCATGGATGGAG+TGG | 0.581114 | 7:-50243037 | MsG0780038636.01.T01:CDS |
| CCATCAGAACCATTAACACG+AGG | 0.697951 | 7:+50242953 | None:intergenic |
| CCTCGTGTTAATGGTTCTGA+TGG | 0.443378 | 7:-50242953 | MsG0780038636.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAGAACATGTTTGAAGAA+GGG | - | Chr7:50242935-50242954 | MsG0780038636.01.T01:CDS | 30.0% | |
| TAAGATGAAGAAGAGTTCTA+AGG | - | Chr7:50242906-50242925 | MsG0780038636.01.T01:CDS | 30.0% | |
| AAAGAGGAAGAAGACAACAT+GGG | - | Chr7:50242992-50243011 | MsG0780038636.01.T01:CDS | 35.0% | |
| AACATGTTTGAAGAAGGGAA+CGG | - | Chr7:50242940-50242959 | MsG0780038636.01.T01:CDS | 35.0% | |
| GATGAGAACATGTTTGAAGA+AGG | - | Chr7:50242934-50242953 | MsG0780038636.01.T01:CDS | 35.0% | |
| AATTTGGCCAAATTCACCAG+TGG | + | Chr7:50243124-50243143 | None:intergenic | 40.0% | |
| ACATGTTTGAAGAAGGGAAC+GGG | - | Chr7:50242941-50242960 | MsG0780038636.01.T01:CDS | 40.0% | |
| AGGGTTGAAGTAGCAATGTT+TGG | - | Chr7:50242964-50242983 | MsG0780038636.01.T01:CDS | 40.0% | |
| GAAAGAGGAAGAAGACAACA+TGG | - | Chr7:50242991-50243010 | MsG0780038636.01.T01:CDS | 40.0% | |
| GTCTCTAATCCTCGTGTTAA+TGG | - | Chr7:50243081-50243100 | MsG0780038636.01.T01:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 50242865 | 50243200 | 50242865 | ID=MsG0780038636.01;Name=MsG0780038636.01 |
| Chr7 | mRNA | 50242865 | 50243200 | 50242865 | ID=MsG0780038636.01.T01;Parent=MsG0780038636.01;Name=MsG0780038636.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|111 |
| Chr7 | exon | 50242865 | 50243200 | 50242865 | ID=MsG0780038636.01.T01:exon:6852;Parent=MsG0780038636.01.T01 |
| Chr7 | CDS | 50242865 | 50243200 | 50242865 | ID=MsG0780038636.01.T01:cds;Parent=MsG0780038636.01.T01 |
| Gene Sequence |
>MsG0780038636.01.T01 ATGTGGCATCTTGTAGCTGCAGTGAGAAGAATTAATATTTATAAGATGAAGAAGAGTTCTAAGGTGGCAGATGAGAACATGTTTGAAGAAGGGAACGGGAGGGTTGAAGTAGCAATGTTTGGAGGTGAAAGAGGAAGAAGACAACATGGGCATGGATGGAGTGGTTTTTCAGTTATTTATGCTATTCTTCAAGCTCCAATTTCAATACTCTCTTGTGTCTCTAATCCTCGTGTTAATGGTTCTGATGGGGTTTGGTCCACTGGTGAATTTGGCCAAATTTCTGAGATCAATCATATTATGGTAAATGATAGTATGAGATATGCAATTTTAATGTAG |
| Protein sequence |
>MsG0780038636.01.T01 MWHLVAAVRRINIYKMKKSSKVADENMFEEGNGRVEVAMFGGERGRRQHGHGWSGFSVIYAILQAPISILSCVSNPRVNGSDGVWSTGEFGQISEINHIMVNDSMRYAILM* |
