AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038636.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038636.01.T01 MTR_7g058970 97.297 111 3 0 1 111 11 121 9.82e-77 222
MsG0780038636.01.T01 MTR_5g088550 63.636 110 31 4 1 109 1 102 6.34e-42 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038636.01.T01 AT5G35732 42.982 114 48 5 1 111 1 100 3.87e-23 86.3
MsG0780038636.01.T01 AT2G04795 36.937 111 54 2 1 111 1 95 3.16e-19 76.3
MsG0780038636.01.T01 AT5G35732 37.736 106 49 5 1 103 1 92 8.53e-16 67.4

Find 28 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGGTCCACTGGTGAATT+TGG 0.162344 7:-50242929 MsG0780038636.01.T01:CDS
GTCTCTAATCCTCGTGTTAA+TGG 0.187618 7:-50242962 MsG0780038636.01.T01:CDS
TATGATTGATCTCAGAAATT+TGG 0.197925 7:+50242906 None:intergenic
TTCTGAGATCAATCATATTA+TGG 0.254714 7:-50242900 MsG0780038636.01.T01:CDS
ACACAAGAGAGTATTGAAAT+TGG 0.270980 7:+50242983 None:intergenic
TCTGATGGGGTTTGGTCCAC+TGG 0.299361 7:-50242938 MsG0780038636.01.T01:CDS
TAAGATGAAGAAGAGTTCTA+AGG 0.334240 7:-50243137 MsG0780038636.01.T01:CDS
AGGGTTGAAGTAGCAATGTT+TGG 0.404594 7:-50243079 MsG0780038636.01.T01:CDS
CCTCGTGTTAATGGTTCTGA+TGG 0.443378 7:-50242953 MsG0780038636.01.T01:CDS
TTAATGGTTCTGATGGGGTT+TGG 0.468172 7:-50242946 MsG0780038636.01.T01:CDS
GCAATGTTTGGAGGTGAAAG+AGG 0.475498 7:-50243067 MsG0780038636.01.T01:CDS
CTCGTGTTAATGGTTCTGAT+GGG 0.485796 7:-50242952 MsG0780038636.01.T01:CDS
ACATGTTTGAAGAAGGGAAC+GGG 0.488785 7:-50243102 MsG0780038636.01.T01:CDS
GATGAGAACATGTTTGAAGA+AGG 0.490830 7:-50243109 MsG0780038636.01.T01:CDS
GAAGACAACATGGGCATGGA+TGG 0.497380 7:-50243042 MsG0780038636.01.T01:CDS
GTTGAAGTAGCAATGTTTGG+AGG 0.558356 7:-50243076 MsG0780038636.01.T01:CDS
ATGAGAACATGTTTGAAGAA+GGG 0.580253 7:-50243108 MsG0780038636.01.T01:CDS
CAACATGGGCATGGATGGAG+TGG 0.581114 7:-50243037 MsG0780038636.01.T01:CDS
GGAAGAAGACAACATGGGCA+TGG 0.581946 7:-50243046 MsG0780038636.01.T01:CDS
TCGTGTTAATGGTTCTGATG+GGG 0.583059 7:-50242951 MsG0780038636.01.T01:CDS
GATGAAGAAGAGTTCTAAGG+TGG 0.623295 7:-50243134 MsG0780038636.01.T01:CDS
GAAAGAGGAAGAAGACAACA+TGG 0.650164 7:-50243052 MsG0780038636.01.T01:CDS
TGTTTGAAGAAGGGAACGGG+AGG 0.657319 7:-50243099 MsG0780038636.01.T01:CDS
AACATGTTTGAAGAAGGGAA+CGG 0.661073 7:-50243103 MsG0780038636.01.T01:CDS
AATTTGGCCAAATTCACCAG+TGG 0.678766 7:+50242922 None:intergenic
GTTTGAAGAAGGGAACGGGA+GGG 0.689169 7:-50243098 MsG0780038636.01.T01:CDS
CCATCAGAACCATTAACACG+AGG 0.697951 7:+50242953 None:intergenic
AAAGAGGAAGAAGACAACAT+GGG 0.729913 7:-50243051 MsG0780038636.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATGATTGATCTCAGAAATT+TGG + Chr7:50243140-50243159 None:intergenic 25.0%
!!! TTCTGAGATCAATCATATTA+TGG - Chr7:50243143-50243162 MsG0780038636.01.T01:CDS 25.0%
ATGAGAACATGTTTGAAGAA+GGG - Chr7:50242935-50242954 MsG0780038636.01.T01:CDS 30.0%
TAAGATGAAGAAGAGTTCTA+AGG - Chr7:50242906-50242925 MsG0780038636.01.T01:CDS 30.0%
!! ACACAAGAGAGTATTGAAAT+TGG + Chr7:50243063-50243082 None:intergenic 30.0%
AAAGAGGAAGAAGACAACAT+GGG - Chr7:50242992-50243011 MsG0780038636.01.T01:CDS 35.0%
AACATGTTTGAAGAAGGGAA+CGG - Chr7:50242940-50242959 MsG0780038636.01.T01:CDS 35.0%
GATGAGAACATGTTTGAAGA+AGG - Chr7:50242934-50242953 MsG0780038636.01.T01:CDS 35.0%
AATTTGGCCAAATTCACCAG+TGG + Chr7:50243124-50243143 None:intergenic 40.0%
ACATGTTTGAAGAAGGGAAC+GGG - Chr7:50242941-50242960 MsG0780038636.01.T01:CDS 40.0%
AGGGTTGAAGTAGCAATGTT+TGG - Chr7:50242964-50242983 MsG0780038636.01.T01:CDS 40.0%
GAAAGAGGAAGAAGACAACA+TGG - Chr7:50242991-50243010 MsG0780038636.01.T01:CDS 40.0%
GTCTCTAATCCTCGTGTTAA+TGG - Chr7:50243081-50243100 MsG0780038636.01.T01:CDS 40.0%
GTTGAAGTAGCAATGTTTGG+AGG - Chr7:50242967-50242986 MsG0780038636.01.T01:CDS 40.0%
!! CTCGTGTTAATGGTTCTGAT+GGG - Chr7:50243091-50243110 MsG0780038636.01.T01:CDS 40.0%
!! GATGAAGAAGAGTTCTAAGG+TGG - Chr7:50242909-50242928 MsG0780038636.01.T01:CDS 40.0%
!! TCGTGTTAATGGTTCTGATG+GGG - Chr7:50243092-50243111 MsG0780038636.01.T01:CDS 40.0%
!! TTAATGGTTCTGATGGGGTT+TGG - Chr7:50243097-50243116 MsG0780038636.01.T01:CDS 40.0%
CCATCAGAACCATTAACACG+AGG + Chr7:50243093-50243112 None:intergenic 45.0%
GCAATGTTTGGAGGTGAAAG+AGG - Chr7:50242976-50242995 MsG0780038636.01.T01:CDS 45.0%
GTTTGGTCCACTGGTGAATT+TGG - Chr7:50243114-50243133 MsG0780038636.01.T01:CDS 45.0%
!! CCTCGTGTTAATGGTTCTGA+TGG - Chr7:50243090-50243109 MsG0780038636.01.T01:CDS 45.0%
GAAGACAACATGGGCATGGA+TGG - Chr7:50243001-50243020 MsG0780038636.01.T01:CDS 50.0%
GGAAGAAGACAACATGGGCA+TGG - Chr7:50242997-50243016 MsG0780038636.01.T01:CDS 50.0%
GTTTGAAGAAGGGAACGGGA+GGG - Chr7:50242945-50242964 MsG0780038636.01.T01:CDS 50.0%
TGTTTGAAGAAGGGAACGGG+AGG - Chr7:50242944-50242963 MsG0780038636.01.T01:CDS 50.0%
CAACATGGGCATGGATGGAG+TGG - Chr7:50243006-50243025 MsG0780038636.01.T01:CDS 55.0%
! TCTGATGGGGTTTGGTCCAC+TGG - Chr7:50243105-50243124 MsG0780038636.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 50242865 50243200 50242865 ID=MsG0780038636.01;Name=MsG0780038636.01
Chr7 mRNA 50242865 50243200 50242865 ID=MsG0780038636.01.T01;Parent=MsG0780038636.01;Name=MsG0780038636.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|111
Chr7 exon 50242865 50243200 50242865 ID=MsG0780038636.01.T01:exon:6852;Parent=MsG0780038636.01.T01
Chr7 CDS 50242865 50243200 50242865 ID=MsG0780038636.01.T01:cds;Parent=MsG0780038636.01.T01
Gene Sequence

>MsG0780038636.01.T01

ATGTGGCATCTTGTAGCTGCAGTGAGAAGAATTAATATTTATAAGATGAAGAAGAGTTCTAAGGTGGCAGATGAGAACATGTTTGAAGAAGGGAACGGGAGGGTTGAAGTAGCAATGTTTGGAGGTGAAAGAGGAAGAAGACAACATGGGCATGGATGGAGTGGTTTTTCAGTTATTTATGCTATTCTTCAAGCTCCAATTTCAATACTCTCTTGTGTCTCTAATCCTCGTGTTAATGGTTCTGATGGGGTTTGGTCCACTGGTGAATTTGGCCAAATTTCTGAGATCAATCATATTATGGTAAATGATAGTATGAGATATGCAATTTTAATGTAG

Protein sequence

>MsG0780038636.01.T01

MWHLVAAVRRINIYKMKKSSKVADENMFEEGNGRVEVAMFGGERGRRQHGHGWSGFSVIYAILQAPISILSCVSNPRVNGSDGVWSTGEFGQISEINHIMVNDSMRYAILM*