AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006049.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006049.01.T01 MTR_1g112700 95.604 182 7 1 13 193 1 182 3.40e-128 358
MsG0180006049.01.T01 MTR_3g099580 40.196 102 59 2 40 141 22 121 1.32e-22 90.1
MsG0180006049.01.T01 MTR_3g099570 33.784 148 82 4 29 172 7 142 1.75e-20 84.7
MsG0180006049.01.T01 MTR_8g007020 36.585 123 72 4 20 140 2 120 3.01e-20 84.0
MsG0180006049.01.T01 MTR_8g007035 38.211 123 70 4 20 140 2 120 1.06e-19 82.8
MsG0180006049.01.T01 MTR_3g099680 36.134 119 70 3 29 142 7 124 4.65e-19 81.6
MsG0180006049.01.T01 MTR_8g089110 37.607 117 67 4 26 140 8 120 6.10e-19 79.0
MsG0180006049.01.T01 MTR_6g013200 36.667 120 72 3 25 141 14 132 1.70e-18 79.0
MsG0180006049.01.T01 MTR_3g099980 35.294 119 71 3 29 142 7 124 5.71e-18 78.6
MsG0180006049.01.T01 MTR_1g104800 32.773 119 73 3 24 140 9 122 7.13e-18 77.8
MsG0180006049.01.T01 MTR_1g090190 33.058 121 71 3 41 161 20 130 1.98e-17 76.3
MsG0180006049.01.T01 MTR_7g086280 30.894 123 80 2 25 145 7 126 3.49e-17 75.9
MsG0180006049.01.T01 MTR_5g006040 31.034 116 77 2 27 140 9 123 3.56e-17 75.9
MsG0180006049.01.T01 MTR_4g114870 37.624 101 58 2 44 140 191 290 1.09e-16 77.4
MsG0180006049.01.T01 MTR_4g114870 31.126 151 87 4 18 164 5 142 2.81e-16 76.3
MsG0180006049.01.T01 MTR_7g086230 29.268 123 82 2 25 145 7 126 2.56e-16 73.6
MsG0180006049.01.T01 MTR_1g105130 35.484 124 77 3 22 143 2 124 5.97e-16 72.0
MsG0180006049.01.T01 MTR_1g105120 35.484 124 77 3 22 143 2 124 5.97e-16 72.0
MsG0180006049.01.T01 MTR_6g080660 30.252 119 81 2 28 146 12 128 1.10e-15 72.4
MsG0180006049.01.T01 MTR_7g086160 34.821 112 70 2 19 130 4 112 2.83e-15 70.5
MsG0180006049.01.T01 MTR_8g088830 38.235 102 60 2 39 140 23 121 3.42e-15 68.9
MsG0180006049.01.T01 MTR_7g086100 33.333 123 75 3 25 143 7 126 5.24e-15 70.9
MsG0180006049.01.T01 MTR_7g086220 32.479 117 77 2 25 140 6 121 7.18e-15 69.3
MsG0180006049.01.T01 MTR_7g086140 33.929 112 70 2 19 130 4 111 7.25e-15 69.3
MsG0180006049.01.T01 MTR_7g086190 33.929 112 70 2 19 130 4 111 7.25e-15 69.3
MsG0180006049.01.T01 MTR_6g013420 33.607 122 77 3 22 141 16 135 8.69e-15 69.3
MsG0180006049.01.T01 MTR_4g007250 34.615 104 66 2 39 142 24 125 1.06e-14 68.9
MsG0180006049.01.T01 MTR_3g089035 37.755 98 57 3 41 138 30 123 1.45e-14 67.4
MsG0180006049.01.T01 MTR_8g463180 29.167 120 82 2 23 140 3 121 3.08e-14 69.7
MsG0180006049.01.T01 MTR_2g088990 31.897 116 73 4 28 139 6 119 9.90e-14 67.4
MsG0180006049.01.T01 MTR_7g086090 32.231 121 77 2 24 142 6 123 1.48e-13 67.4
MsG0180006049.01.T01 MTR_6g023760 33.981 103 65 3 39 140 21 121 1.97e-13 65.9
MsG0180006049.01.T01 MTR_3g089005 34.188 117 71 4 20 136 11 121 2.16e-13 64.3
MsG0180006049.01.T01 MTR_4g067200 26.230 122 85 2 28 144 12 133 3.88e-13 64.7
MsG0180006049.01.T01 MTR_4g066110 26.230 122 85 2 28 144 12 133 3.88e-13 64.7
MsG0180006049.01.T01 MTR_4g130780 29.078 141 87 4 24 159 9 141 1.17e-12 63.9
MsG0180006049.01.T01 MTR_6g013170 27.397 146 90 3 23 166 8 139 2.44e-12 62.8
MsG0180006049.01.T01 MTR_2g043730 32.773 119 76 3 22 140 10 124 3.27e-12 61.2
MsG0180006049.01.T01 MTR_2g090575 30.833 120 78 3 23 140 6 122 1.55e-11 62.4
MsG0180006049.01.T01 MTR_8g099220 29.268 123 80 3 25 141 7 128 4.70e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006049.01.T01 AT2G31050 37.333 150 81 4 15 163 1 138 1.04e-23 93.6
MsG0180006049.01.T01 AT3G17675 34.454 119 73 2 26 140 10 127 8.10e-20 81.3
MsG0180006049.01.T01 AT5G26330 35.294 102 63 2 41 140 21 121 1.95e-18 79.3
MsG0180006049.01.T01 AT2G26720 35.878 131 81 3 15 144 1 129 7.14e-18 78.2
MsG0180006049.01.T01 AT2G32300 27.673 159 108 2 24 182 6 157 1.39e-17 78.6
MsG0180006049.01.T01 AT2G32300 27.673 159 108 2 24 182 36 187 1.89e-17 78.6
MsG0180006049.01.T01 AT1G72230 31.884 138 86 4 19 156 3 132 7.69e-17 75.1
MsG0180006049.01.T01 AT5G07475 28.767 146 100 3 23 166 9 152 1.39e-16 74.7
MsG0180006049.01.T01 AT2G02850 35.088 114 66 4 25 138 21 126 1.82e-16 72.8
MsG0180006049.01.T01 AT2G25060 28.767 146 94 4 27 163 14 158 2.91e-15 70.9
MsG0180006049.01.T01 AT5G15350 28.235 170 102 5 23 173 4 172 3.95e-15 70.1
MsG0180006049.01.T01 AT5G20230 30.645 124 82 2 44 164 25 147 3.82e-14 68.2
MsG0180006049.01.T01 AT1G22480 32.773 119 78 2 29 147 4 120 4.99e-14 67.4
MsG0180006049.01.T01 AT4G31840 27.273 154 97 5 20 162 3 152 5.68e-14 67.4
MsG0180006049.01.T01 AT3G60270 33.981 103 66 2 38 140 20 120 9.48e-14 67.0
MsG0180006049.01.T01 AT4G32490 26.667 120 85 2 23 140 10 128 2.66e-13 66.2
MsG0180006049.01.T01 AT2G27035 28.448 116 81 2 47 161 32 146 2.73e-13 65.1
MsG0180006049.01.T01 AT1G45063 32.800 125 75 4 24 143 9 129 3.48e-13 66.6
MsG0180006049.01.T01 AT1G17800 30.833 120 78 2 24 139 17 135 6.02e-13 63.5
MsG0180006049.01.T01 AT1G45063 32.800 125 75 4 24 143 9 129 8.04e-13 66.2
MsG0180006049.01.T01 AT3G27200 30.508 118 79 3 24 140 6 121 8.09e-13 63.9
MsG0180006049.01.T01 AT4G12880 31.624 117 76 3 27 141 12 126 1.22e-12 62.8
MsG0180006049.01.T01 AT5G53870 30.000 160 97 6 24 170 1 158 1.43e-12 65.5
MsG0180006049.01.T01 AT5G53870 30.000 160 97 6 24 170 1 158 1.76e-12 65.5
MsG0180006049.01.T01 AT4G28365 31.313 99 65 2 44 140 29 126 5.90e-12 62.4
MsG0180006049.01.T01 AT3G60280 32.039 103 68 2 38 140 18 118 8.11e-12 62.4
MsG0180006049.01.T01 AT3G20570 29.545 132 82 3 29 150 8 138 2.01e-11 60.8
MsG0180006049.01.T01 AT3G18590 29.839 124 76 5 24 140 7 126 4.56e-11 59.7
MsG0180006049.01.T01 AT2G44790 33.333 96 60 3 51 144 35 128 6.94e-11 59.3

Find 40 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGTGATCACCATTTCTT+TGG 0.227237 1:+99372381 None:intergenic
TGCCTTGGAGGAATGAGGTT+TGG 0.332332 1:-99371778 MsG0180006049.01.T01:CDS
CCCATGTAGCATAATTTGTT+TGG 0.336122 1:+99372248 None:intergenic
GCCTTGGAGGAATGAGGTTT+GGG 0.340464 1:-99371777 MsG0180006049.01.T01:CDS
AGATCAAGTGGTTCTAAATA+AGG 0.343342 1:-99371839 MsG0180006049.01.T01:CDS
AAGTTAGTTCCTTTCGGAAC+TGG 0.360694 1:-99371634 MsG0180006049.01.T01:CDS
TTCTTACCTTTCCAAAGAAA+TGG 0.367475 1:-99372392 MsG0180006049.01.T01:CDS
TAAATAAGGTGAAGAGATAT+TGG 0.384569 1:-99371825 MsG0180006049.01.T01:CDS
TCTCAAAGATGGAGAGTGAT+TGG 0.389696 1:-99371658 MsG0180006049.01.T01:CDS
TTTAGCCGTGGAGATGTTCT+TGG 0.401037 1:-99372211 MsG0180006049.01.T01:intron
ATTGGGAAGTTAGTTCCTTT+CGG 0.402044 1:-99371640 MsG0180006049.01.T01:CDS
GATGGAGTTGGTTGAATTTG+TGG 0.412161 1:+99371697 None:intergenic
TGGTTCATTTGCAATATAGC+TGG 0.431102 1:-99371805 MsG0180006049.01.T01:CDS
TGGATTTAATGCACCGTCCA+TGG 0.449414 1:+99371717 None:intergenic
ATAGCTGGACATTGCCTTGG+AGG 0.459542 1:-99371790 MsG0180006049.01.T01:CDS
ATGGCTAGTGTTTATGCTGT+TGG 0.480177 1:-99372292 MsG0180006049.01.T01:CDS
CTCAAAGATGGAGAGTGATT+GGG 0.483596 1:-99371657 MsG0180006049.01.T01:CDS
ATTCTTGTATCTCTCAAAGA+TGG 0.491476 1:-99371669 MsG0180006049.01.T01:CDS
GACATTGCCTTGGAGGAATG+AGG 0.494262 1:-99371783 MsG0180006049.01.T01:CDS
TGATCACCATTTCTTTGGAA+AGG 0.494858 1:+99372386 None:intergenic
CAAGAATTTAGTGATGGAGT+TGG 0.498793 1:+99371685 None:intergenic
GCCAAACAAATTATGCTACA+TGG 0.511548 1:-99372249 MsG0180006049.01.T01:CDS
GAGATACAAGAATTTAGTGA+TGG 0.532359 1:+99371679 None:intergenic
CAACAATGGTACTAATTCCA+TGG 0.540787 1:-99371734 MsG0180006049.01.T01:CDS
AATATAGCTGGACATTGCCT+TGG 0.550956 1:-99371793 MsG0180006049.01.T01:CDS
CTGTTGGTGATCAAGATGAA+TGG 0.566698 1:-99372276 MsG0180006049.01.T01:CDS
AATAAGAATTGAGAGTATGA+AGG 0.571197 1:+99372345 None:intergenic
GTGTTGGCTAAGTATGAAAG+TGG 0.577082 1:-99371865 MsG0180006049.01.T01:CDS
GTCAAAGAAGATAGCAACAA+TGG 0.584763 1:-99371748 MsG0180006049.01.T01:CDS
TAATGTGTATGAAGTGAGAG+AGG 0.584918 1:-99371920 MsG0180006049.01.T01:CDS
CCAAACAAATTATGCTACAT+GGG 0.597055 1:-99372248 MsG0180006049.01.T01:CDS
AATAATAATCCAGTTCCGAA+AGG 0.605908 1:+99371625 None:intergenic
TGAAACAAGTAGTGGAGTGT+TGG 0.609215 1:-99371881 MsG0180006049.01.T01:CDS
AGATCATGTGAAACAAGTAG+TGG 0.611448 1:-99371889 MsG0180006049.01.T01:CDS
AATGGTACTAATTCCATGGA+CGG 0.621827 1:-99371730 MsG0180006049.01.T01:CDS
GAGAGATACAACTTTAGCCG+TGG 0.623416 1:-99372223 MsG0180006049.01.T01:CDS
TCCCAAACCTCATTCCTCCA+AGG 0.624087 1:+99371776 None:intergenic
TGAAAGTGGAGAAGATCAAG+TGG 0.654735 1:-99371851 MsG0180006049.01.T01:CDS
TACTACCAAGAACATCTCCA+CGG 0.676544 1:+99372206 None:intergenic
AAGAAGATGTGATGAAACAG+TGG 0.725138 1:+99372410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAATTAAATATTTTT+TGG - Chr1:99372031-99372050 MsG0180006049.01.T01:intron 0.0%
!!! AATATTTTTTGGTGTTGTAT+TGG - Chr1:99372042-99372061 MsG0180006049.01.T01:intron 20.0%
! AAGATAATGTTATGATCACA+TGG + Chr1:99371918-99371937 None:intergenic 25.0%
! TAAATAAGGTGAAGAGATAT+TGG - Chr1:99372190-99372209 MsG0180006049.01.T01:intron 25.0%
!! AATAAGAATTGAGAGTATGA+AGG + Chr1:99371673-99371692 None:intergenic 25.0%
!! ATCACATGGATATCTTTTAT+TGG + Chr1:99371904-99371923 None:intergenic 25.0%
!!! ATAGTTTTCAAGTATGTGAA+GGG - Chr1:99372066-99372085 MsG0180006049.01.T01:intron 25.0%
!!! TATAGTTTTCAAGTATGTGA+AGG - Chr1:99372065-99372084 MsG0180006049.01.T01:intron 25.0%
AATAATAATCCAGTTCCGAA+AGG + Chr1:99372393-99372412 None:intergenic 30.0%
ATTCTTGTATCTCTCAAAGA+TGG - Chr1:99372346-99372365 MsG0180006049.01.T01:CDS 30.0%
CCAAACAAATTATGCTACAT+GGG - Chr1:99371767-99371786 MsG0180006049.01.T01:CDS 30.0%
GAGATACAAGAATTTAGTGA+TGG + Chr1:99372339-99372358 None:intergenic 30.0%
TTCTTACCTTTCCAAAGAAA+TGG - Chr1:99371623-99371642 MsG0180006049.01.T01:CDS 30.0%
! TTTTCTCTTGAATTGTGTCA+TGG - Chr1:99371704-99371723 MsG0180006049.01.T01:CDS 30.0%
!! AGATCAAGTGGTTCTAAATA+AGG - Chr1:99372176-99372195 MsG0180006049.01.T01:intron 30.0%
!!! TAGTTTTCAAGTATGTGAAG+GGG - Chr1:99372067-99372086 MsG0180006049.01.T01:intron 30.0%
AATGGTACTAATTCCATGGA+CGG - Chr1:99372285-99372304 MsG0180006049.01.T01:CDS 35.0%
AGATCATGTGAAACAAGTAG+TGG - Chr1:99372126-99372145 MsG0180006049.01.T01:intron 35.0%
ATTGGGAAGTTAGTTCCTTT+CGG - Chr1:99372375-99372394 MsG0180006049.01.T01:CDS 35.0%
CAACAATGGTACTAATTCCA+TGG - Chr1:99372281-99372300 MsG0180006049.01.T01:CDS 35.0%
GAAAGTGATCACCATTTCTT+TGG + Chr1:99371637-99371656 None:intergenic 35.0%
GCCAAACAAATTATGCTACA+TGG - Chr1:99371766-99371785 MsG0180006049.01.T01:CDS 35.0%
GTCAAAGAAGATAGCAACAA+TGG - Chr1:99372267-99372286 MsG0180006049.01.T01:CDS 35.0%
TAATGTGTATGAAGTGAGAG+AGG - Chr1:99372095-99372114 MsG0180006049.01.T01:intron 35.0%
TGGTTCATTTGCAATATAGC+TGG - Chr1:99372210-99372229 MsG0180006049.01.T01:intron 35.0%
! CAAGAATTTAGTGATGGAGT+TGG + Chr1:99372333-99372352 None:intergenic 35.0%
! TGATCACCATTTCTTTGGAA+AGG + Chr1:99371632-99371651 None:intergenic 35.0%
!! CCCATGTAGCATAATTTGTT+TGG + Chr1:99371770-99371789 None:intergenic 35.0%
AAGTTAGTTCCTTTCGGAAC+TGG - Chr1:99372381-99372400 MsG0180006049.01.T01:CDS 40.0%
AATATAGCTGGACATTGCCT+TGG - Chr1:99372222-99372241 MsG0180006049.01.T01:CDS 40.0%
ATGGCTAGTGTTTATGCTGT+TGG - Chr1:99371723-99371742 MsG0180006049.01.T01:CDS 40.0%
CTCAAAGATGGAGAGTGATT+GGG - Chr1:99372358-99372377 MsG0180006049.01.T01:CDS 40.0%
TACTACCAAGAACATCTCCA+CGG + Chr1:99371812-99371831 None:intergenic 40.0%
TCTCAAAGATGGAGAGTGAT+TGG - Chr1:99372357-99372376 MsG0180006049.01.T01:CDS 40.0%
TGAAAGTGGAGAAGATCAAG+TGG - Chr1:99372164-99372183 MsG0180006049.01.T01:intron 40.0%
! GATGGAGTTGGTTGAATTTG+TGG + Chr1:99372321-99372340 None:intergenic 40.0%
! TGAAACAAGTAGTGGAGTGT+TGG - Chr1:99372134-99372153 MsG0180006049.01.T01:intron 40.0%
!! CTGTTGGTGATCAAGATGAA+TGG - Chr1:99371739-99371758 MsG0180006049.01.T01:CDS 40.0%
!! GTGTTGGCTAAGTATGAAAG+TGG - Chr1:99372150-99372169 MsG0180006049.01.T01:intron 40.0%
GAGAGATACAACTTTAGCCG+TGG - Chr1:99371792-99371811 MsG0180006049.01.T01:CDS 45.0%
TGGATTTAATGCACCGTCCA+TGG + Chr1:99372301-99372320 None:intergenic 45.0%
TTTAGCCGTGGAGATGTTCT+TGG - Chr1:99371804-99371823 MsG0180006049.01.T01:CDS 45.0%
ATAGCTGGACATTGCCTTGG+AGG - Chr1:99372225-99372244 MsG0180006049.01.T01:CDS 50.0%
GACATTGCCTTGGAGGAATG+AGG - Chr1:99372232-99372251 MsG0180006049.01.T01:CDS 50.0%
GCCTTGGAGGAATGAGGTTT+GGG - Chr1:99372238-99372257 MsG0180006049.01.T01:CDS 50.0%
TCCCAAACCTCATTCCTCCA+AGG + Chr1:99372242-99372261 None:intergenic 50.0%
TGCCTTGGAGGAATGAGGTT+TGG - Chr1:99372237-99372256 MsG0180006049.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 99371606 99372431 99371606 ID=MsG0180006049.01;Name=MsG0180006049.01
Chr1 mRNA 99371606 99372431 99371606 ID=MsG0180006049.01.T01;Parent=MsG0180006049.01;Name=MsG0180006049.01.T01;_AED=0.51;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|193
Chr1 exon 99372212 99372431 99372212 ID=MsG0180006049.01.T01:exon:9955;Parent=MsG0180006049.01.T01
Chr1 exon 99371606 99371967 99371606 ID=MsG0180006049.01.T01:exon:9954;Parent=MsG0180006049.01.T01
Chr1 CDS 99372212 99372431 99372212 ID=MsG0180006049.01.T01:cds;Parent=MsG0180006049.01.T01
Chr1 CDS 99371606 99371967 99371606 ID=MsG0180006049.01.T01:cds;Parent=MsG0180006049.01.T01
Gene Sequence

>MsG0180006049.01.T01

CACTGTTTCATCACATCTTCTTACCTTTCCAAAGAAATGGTGATCACTTTCTTCAACAACTGTACCTTCATACTCTCAATTCTTATTTTGCTATGTAGTTTTCTCTTGAATTGTGTCATGGCTAGTGTTTATGCTGTTGGTGATCAAGATGAATGGAGTAGCCAAACAAATTATGCTACATGGGCAGAGAGATACAACTTTAGCCGTGGAGATGTTCTTGTTTTCAAGTATGTGAAGGGGCAACATAATGTGTATGAAGTGAGAGAGGAAACTTTTAGATCATGTGAAACAAGTAGTGGAGTGTTGGCTAAGTATGAAAGTGGAGAAGATCAAGTGGTTCTAAATAAGGTGAAGAGATATTGGTTCATTTGCAATATAGCTGGACATTGCCTTGGAGGAATGAGGTTTGGGATTGAAGTCAAAGAAGATAGCAACAATGGTACTAATTCCATGGACGGTGCATTAAATCCACAAATTCAACCAACTCCATCACTAAATTCTTGTATCTCTCAAAGATGGAGAGTGATTGGGAAGTTAGTTCCTTTCGGAACTGGATTATTATTGCTCAATTTGTATTTTTGA

Protein sequence

>MsG0180006049.01.T01

HCFITSSYLSKEMVITFFNNCTFILSILILLCSFLLNCVMASVYAVGDQDEWSSQTNYATWAERYNFSRGDVLVFKYVKGQHNVYEVREETFRSCETSSGVLAKYESGEDQVVLNKVKRYWFICNIAGHCLGGMRFGIEVKEDSNNGTNSMDGALNPQIQPTPSLNSCISQRWRVIGKLVPFGTGLLLLNLYF*