AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047473.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047473.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047473.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0880047473.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047473.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047473.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 31 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGGAGGACTCTTTA+TGG 0.255446 8:+86879236 MsG0880047473.01.T01:CDS
GGGAGGACTCTTTATGGATT+TGG 0.328696 8:+86879242 MsG0880047473.01.T01:CDS
GGCAAAGGAGGAAAGGGTTT+AGG 0.384869 8:+86878972 MsG0880047473.01.T01:CDS
GGAGGAAAGGGTTTAGGAAA+GGG 0.429069 8:+86878978 MsG0880047473.01.T01:CDS
TCTTTATGGATTTGGCGGTT+AGG 0.439485 8:+86879250 MsG0880047473.01.T01:CDS
TCGCAGGCTTTGTAATTCCC+TGG 0.486609 8:-86879040 None:intergenic
TGTTTACGCTCTCAAGAGGC+AGG 0.493747 8:+86879220 MsG0880047473.01.T01:CDS
AGGACTCTTTATGGATTTGG+CGG 0.515922 8:+86879245 MsG0880047473.01.T01:CDS
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 8:+86878965 MsG0880047473.01.T01:CDS
GGTTCTTCGTGATAACATCC+AGG 0.522451 8:+86879022 MsG0880047473.01.T01:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 8:+86878966 MsG0880047473.01.T01:CDS
AGGAGGAAAGGGTTTAGGAA+AGG 0.528235 8:+86878977 MsG0880047473.01.T01:CDS
GGAAAGGGTTTAGGAAAGGG+AGG 0.536831 8:+86878981 MsG0880047473.01.T01:CDS
CCATCGCAGTAACAGTCTTA+CGG 0.545551 8:-86879193 None:intergenic
GCGATGTCAGGAAGAGGCAA+AGG 0.557914 8:+86878957 None:intergenic
CCGTAAGACTGTTACTGCGA+TGG 0.561095 8:+86879193 MsG0880047473.01.T01:CDS
TGTTATCACGAAGAACCTTA+CGG 0.569497 8:-86879016 None:intergenic
TTCACAGCGATGTCAGGAAG+AGG 0.573275 8:+86878951 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 8:+86879001 MsG0880047473.01.T01:CDS
TTTACGCTCTCAAGAGGCAG+GGG 0.581284 8:+86879222 MsG0880047473.01.T01:CDS
CTTGCAAGACGACGAATCGC+AGG 0.588436 8:-86879056 None:intergenic
ACGGCGAGCATGCTCTGTGT+AGG 0.590446 8:-86879174 None:intergenic
GTTTACGCTCTCAAGAGGCA+GGG 0.591999 8:+86879221 MsG0880047473.01.T01:CDS
ATGTCAGGAAGAGGCAAAGG+AGG 0.592835 8:+86878960 MsG0880047473.01.T01:CDS
ACGCTCTCAAGAGGCAGGGG+AGG 0.605625 8:+86879225 MsG0880047473.01.T01:CDS
ATGTTGTTTACGCTCTCAAG+AGG 0.616421 8:+86879216 MsG0880047473.01.T01:CDS
GTTCTTCGTGATAACATCCA+GGG 0.626731 8:+86879023 MsG0880047473.01.T01:CDS
CGTCGTCTTGCAAGACGTGG+TGG 0.633046 8:+86879065 MsG0880047473.01.T01:CDS
ATTCGTCGTCTTGCAAGACG+TGG 0.646636 8:+86879062 MsG0880047473.01.T01:CDS
TTAATCTATGAAGAAACTCG+TGG 0.649567 8:+86879107 MsG0880047473.01.T01:CDS
GGTGGTGTGAAACGTATCAG+TGG 0.728069 8:+86879083 MsG0880047473.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG + Chr8:86879107-86879126 MsG0880047473.01.T01:CDS 30.0%
TGTTATCACGAAGAACCTTA+CGG - Chr8:86879019-86879038 None:intergenic 35.0%
AGGACTCTTTATGGATTTGG+CGG + Chr8:86879245-86879264 MsG0880047473.01.T01:CDS 40.0%
GTTCTTCGTGATAACATCCA+GGG + Chr8:86879023-86879042 MsG0880047473.01.T01:CDS 40.0%
! ATGTTGTTTACGCTCTCAAG+AGG + Chr8:86879216-86879235 MsG0880047473.01.T01:CDS 40.0%
AGGAGGAAAGGGTTTAGGAA+AGG + Chr8:86878977-86878996 MsG0880047473.01.T01:CDS 45.0%
CCATCGCAGTAACAGTCTTA+CGG - Chr8:86879196-86879215 None:intergenic 45.0%
GGAGGAAAGGGTTTAGGAAA+GGG + Chr8:86878978-86878997 MsG0880047473.01.T01:CDS 45.0%
GGGAGGACTCTTTATGGATT+TGG + Chr8:86879242-86879261 MsG0880047473.01.T01:CDS 45.0%
GGTTCTTCGTGATAACATCC+AGG + Chr8:86879022-86879041 MsG0880047473.01.T01:CDS 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG + Chr8:86878965-86878984 MsG0880047473.01.T01:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG + Chr8:86879001-86879020 MsG0880047473.01.T01:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG + Chr8:86878960-86878979 MsG0880047473.01.T01:CDS 50.0%
ATTCGTCGTCTTGCAAGACG+TGG + Chr8:86879062-86879081 MsG0880047473.01.T01:CDS 50.0%
CCGTAAGACTGTTACTGCGA+TGG + Chr8:86879193-86879212 MsG0880047473.01.T01:CDS 50.0%
GGAAAGGGTTTAGGAAAGGG+AGG + Chr8:86878981-86879000 MsG0880047473.01.T01:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG + Chr8:86878966-86878985 MsG0880047473.01.T01:CDS 50.0%
GGCAAAGGAGGAAAGGGTTT+AGG + Chr8:86878972-86878991 MsG0880047473.01.T01:CDS 50.0%
GGTGGTGTGAAACGTATCAG+TGG + Chr8:86879083-86879102 MsG0880047473.01.T01:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG + Chr8:86879221-86879240 MsG0880047473.01.T01:CDS 50.0%
TGTTTACGCTCTCAAGAGGC+AGG + Chr8:86879220-86879239 MsG0880047473.01.T01:CDS 50.0%
TTTACGCTCTCAAGAGGCAG+GGG + Chr8:86879222-86879241 MsG0880047473.01.T01:CDS 50.0%
! TCGCAGGCTTTGTAATTCCC+TGG - Chr8:86879043-86879062 None:intergenic 50.0%
AGGCAGGGGAGGACTCTTTA+TGG + Chr8:86879236-86879255 MsG0880047473.01.T01:CDS 55.0%
CTTGCAAGACGACGAATCGC+AGG - Chr8:86879059-86879078 None:intergenic 55.0%
ACGGCGAGCATGCTCTGTGT+AGG - Chr8:86879177-86879196 None:intergenic 60.0%
CGTCGTCTTGCAAGACGTGG+TGG + Chr8:86879065-86879084 MsG0880047473.01.T01:CDS 60.0%
ACGCTCTCAAGAGGCAGGGG+AGG + Chr8:86879225-86879244 MsG0880047473.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86878960 86879271 86878960 ID=MsG0880047473.01;Name=MsG0880047473.01
Chr8 mRNA 86878960 86879271 86878960 ID=MsG0880047473.01.T01;Parent=MsG0880047473.01;Name=MsG0880047473.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103
Chr8 exon 86878960 86879271 86878960 ID=MsG0880047473.01.T01:exon:17952;Parent=MsG0880047473.01.T01
Chr8 CDS 86878960 86879271 86878960 ID=MsG0880047473.01.T01:cds;Parent=MsG0880047473.01.T01
Gene Sequence

>MsG0880047473.01.T01

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTTTAGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGTGATAACATCCAGGGAATTACAAAGCCTGCGATTCGTCGTCTTGCAAGACGTGGTGGTGTGAAACGTATCAGTGGTTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAACGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTTGTTTACGCTCTCAAGAGGCAGGGGAGGACTCTTTATGGATTTGGCGGTTAG

Protein sequence

>MsG0880047473.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*