AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025587.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025587.01.T01 MTR_5g030935 100.000 73 0 0 1 73 1 73 1.18e-47 147
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025587.01.T01 AT2G22640 93.151 73 5 0 1 73 1 73 2.19e-45 142

Find 26 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTTGATTGCCAATTTAT+TGG 0.221769 5:-20895581 MsG0580025587.01.T01:three_prime_UTR
CATTGGTTTATTTGCAAATT+CGG 0.278022 5:-20895539 MsG0580025587.01.T01:three_prime_UTR
CAATTTATTGGGTTATTAGA+TGG 0.289266 5:-20895569 MsG0580025587.01.T01:three_prime_UTR
TTCTTGATTGCCAATTTATT+GGG 0.290000 5:-20895580 MsG0580025587.01.T01:three_prime_UTR
CATCTAATAACCCAATAAAT+TGG 0.317606 5:+20895570 None:intergenic
CCATCTAGATTATACATTAT+AGG 0.320418 5:+20895689 None:intergenic
CCTCCAGCACGTGCCATTGA+TGG 0.347571 5:+20896103 None:intergenic
GAAGAAATTCAGTACCGAAT+TGG 0.368507 5:+20895972 None:intergenic
TTGATGTTTAAGGGTATGTT+TGG 0.379817 5:+20896267 None:intergenic
GAATCGCTGTTCAAGCTGAT+TGG 0.386107 5:-20896057 MsG0580025587.01.T01:CDS
CCTATAATGTATAATCTAGA+TGG 0.388438 5:-20895689 MsG0580025587.01.T01:CDS
TGATGTATTGGAGCGTCGTT+TGG 0.391324 5:-20895819 MsG0580025587.01.T01:CDS
TTCGATTTCCTTGTCCAATT+CGG 0.396293 5:-20895986 MsG0580025587.01.T01:intron
TGAAGTAAAGGAGAGTTAGT+TGG 0.400349 5:-20896196 MsG0580025587.01.T01:five_prime_UTR
AAGTTCAAGTTGAAATGCTT+CGG 0.412595 5:-20895787 MsG0580025587.01.T01:CDS
GAATGAAAAGCTTGATGTAT+TGG 0.415813 5:-20895831 MsG0580025587.01.T01:CDS
TATTAGATGGTTTAATGCAT+TGG 0.431631 5:-20895556 MsG0580025587.01.T01:three_prime_UTR
AATCGCTGTTCAAGCTGATT+GGG 0.456401 5:-20896056 MsG0580025587.01.T01:CDS
ATTCCATCAATGGCACGTGC+TGG 0.500686 5:-20896106 MsG0580025587.01.T01:exon
AATAAAATCAAAATCTACAT+TGG 0.509747 5:-20896144 MsG0580025587.01.T01:five_prime_UTR
AAATCAAAGAATTCCATCAA+TGG 0.510384 5:-20896116 MsG0580025587.01.T01:exon
AGATTATACATTATAGGTGG+TGG 0.524893 5:+20895695 None:intergenic
CCATCAATGGCACGTGCTGG+AGG 0.587599 5:-20896103 MsG0580025587.01.T01:exon
ATTCAGTACCGAATTGGACA+AGG 0.614288 5:+20895978 None:intergenic
ATCACAAACGCAGTTAACGT+TGG 0.624596 5:-20896079 MsG0580025587.01.T01:CDS
TCTAGATTATACATTATAGG+TGG 0.659426 5:+20895692 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTATATCTTTTTTTGAT+GGG - Chr5:20895892-20895911 MsG0580025587.01.T01:intron 10.0%
!! AATAAAATCAAAATCTACAT+TGG - Chr5:20895657-20895676 MsG0580025587.01.T01:exon 15.0%
!!! GAAATTATATCTTTTTTTGA+TGG - Chr5:20895891-20895910 MsG0580025587.01.T01:intron 15.0%
!! AGATATAATTTCAACTAACA+AGG + Chr5:20895883-20895902 None:intergenic 20.0%
!!! TGTTATTTTTGTTATTTCAG+AGG - Chr5:20895919-20895938 MsG0580025587.01.T01:intron 20.0%
! AAATCAAAGAATTCCATCAA+TGG - Chr5:20895685-20895704 MsG0580025587.01.T01:CDS 25.0%
! AACAAAAAGTCTTGAAGTAA+AGG - Chr5:20895593-20895612 MsG0580025587.01.T01:three_prime_UTR 25.0%
! CAATTTATTGGGTTATTAGA+TGG - Chr5:20896232-20896251 MsG0580025587.01.T01:five_prime_UTR 25.0%
! CATCTAATAACCCAATAAAT+TGG + Chr5:20896234-20896253 None:intergenic 25.0%
! CCATCTAGATTATACATTAT+AGG + Chr5:20896115-20896134 None:intergenic 25.0%
! CCTATAATGTATAATCTAGA+TGG - Chr5:20896112-20896131 MsG0580025587.01.T01:exon 25.0%
! TATTAGATGGTTTAATGCAT+TGG - Chr5:20896245-20896264 MsG0580025587.01.T01:five_prime_UTR 25.0%
! TCTAGATTATACATTATAGG+TGG + Chr5:20896112-20896131 None:intergenic 25.0%
! TTCTTGATTGCCAATTTATT+GGG - Chr5:20896221-20896240 MsG0580025587.01.T01:five_prime_UTR 25.0%
!! ACTCAAACTTTTGATGTTTA+AGG + Chr5:20895547-20895566 None:intergenic 25.0%
!! CTCAAACTTTTGATGTTTAA+GGG + Chr5:20895546-20895565 None:intergenic 25.0%
!!! CATTGGTTTATTTGCAAATT+CGG - Chr5:20896262-20896281 MsG0580025587.01.T01:five_prime_UTR 25.0%
AGATTATACATTATAGGTGG+TGG + Chr5:20896109-20896128 None:intergenic 30.0%
ATTTCAACTAACAAGGCAAA+AGG + Chr5:20895876-20895895 None:intergenic 30.0%
GAATGAAAAGCTTGATGTAT+TGG - Chr5:20895970-20895989 MsG0580025587.01.T01:intron 30.0%
GGGTATGTTTGGTAAAAAAA+TGG + Chr5:20895526-20895545 None:intergenic 30.0%
GTTCTTGATTGCCAATTTAT+TGG - Chr5:20896220-20896239 MsG0580025587.01.T01:five_prime_UTR 30.0%
TTTCAACTAACAAGGCAAAA+GGG + Chr5:20895875-20895894 None:intergenic 30.0%
! TTGATGTTTAAGGGTATGTT+TGG + Chr5:20895537-20895556 None:intergenic 30.0%
!! AAGTTCAAGTTGAAATGCTT+CGG - Chr5:20896014-20896033 MsG0580025587.01.T01:CDS 30.0%
TGAAGTAAAGGAGAGTTAGT+TGG - Chr5:20895605-20895624 MsG0580025587.01.T01:three_prime_UTR 35.0%
TTCGATTTCCTTGTCCAATT+CGG - Chr5:20895815-20895834 MsG0580025587.01.T01:CDS 35.0%
! CATCAAGTTGCAAAAAGAGA+AGG + Chr5:20896044-20896063 None:intergenic 35.0%
! GAAGAAATTCAGTACCGAAT+TGG + Chr5:20895832-20895851 None:intergenic 35.0%
AATCGCTGTTCAAGCTGATT+GGG - Chr5:20895745-20895764 MsG0580025587.01.T01:CDS 40.0%
ATCACAAACGCAGTTAACGT+TGG - Chr5:20895722-20895741 MsG0580025587.01.T01:CDS 40.0%
! ATTCAGTACCGAATTGGACA+AGG + Chr5:20895826-20895845 None:intergenic 40.0%
GAATCGCTGTTCAAGCTGAT+TGG - Chr5:20895744-20895763 MsG0580025587.01.T01:CDS 45.0%
!! TGATGTATTGGAGCGTCGTT+TGG - Chr5:20895982-20896001 MsG0580025587.01.T01:intron 45.0%
ATTCCATCAATGGCACGTGC+TGG - Chr5:20895695-20895714 MsG0580025587.01.T01:CDS 50.0%
CCATCAATGGCACGTGCTGG+AGG - Chr5:20895698-20895717 MsG0580025587.01.T01:CDS 60.0%
! CCTCCAGCACGTGCCATTGA+TGG + Chr5:20895701-20895720 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 20895517 20896306 20895517 ID=MsG0580025587.01;Name=MsG0580025587.01
Chr5 mRNA 20895517 20896306 20895517 ID=MsG0580025587.01.T01;Parent=MsG0580025587.01;Name=MsG0580025587.01.T01;_AED=0.13;_eAED=0.13;_QI=187|1|1|1|1|1|2|156|114
Chr5 exon 20895517 20895884 20895517 ID=MsG0580025587.01.T01:exon:23404;Parent=MsG0580025587.01.T01
Chr5 exon 20895987 20896306 20895987 ID=MsG0580025587.01.T01:exon:23403;Parent=MsG0580025587.01.T01
Chr5 five_prime_UTR 20896120 20896306 20896120 ID=MsG0580025587.01.T01:five_prime_utr;Parent=MsG0580025587.01.T01
Chr5 CDS 20895987 20896119 20895987 ID=MsG0580025587.01.T01:cds;Parent=MsG0580025587.01.T01
Chr5 CDS 20895673 20895884 20895673 ID=MsG0580025587.01.T01:cds;Parent=MsG0580025587.01.T01
Chr5 three_prime_UTR 20895517 20895672 20895517 ID=MsG0580025587.01.T01:three_prime_utr;Parent=MsG0580025587.01.T01
Gene Sequence

>MsG0580025587.01.T01

ATGGCACGTGCTGGAGGAATCACAAACGCAGTTAACGTTGGAATCGCTGTTCAAGCTGATTGGGAAAATCGTGAATTCATCTCTCACATTTCTCTCAATGTTCGTCGTCTCTTCGATTTCCTTGTCCAATTCGAGGCTACAACAAAGAGTAAATTAGCTTCATTGAATGAAAAGCTTGATGTATTGGAGCGTCGTTTGGAGTTACTTGAAGTTCAAGTTGAAATGCTTCGGCAAACCTTCTCTTTTTGCAACTTGATGTGTGTAATGAATCATGTAATGCAACTTTCTAGTAAGATTTCTCCACCACCTATAATGTATAATCTAGATGGAATTCTTGATCTATAA

Protein sequence

>MsG0580025587.01.T01

MARAGGITNAVNVGIAVQADWENREFISHISLNVRRLFDFLVQFEATTKSKLASLNEKLDVLERRLELLEVQVEMLRQTFSFCNLMCVMNHVMQLSSKISPPPIMYNLDGILDL*