AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006486.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006486.01.T01 MTR_2g009500 97.351 302 8 0 1 302 1 302 0.0 616
MsG0280006486.01.T01 MTR_5g066060 51.475 305 132 7 7 301 10 308 3.32e-100 296
MsG0280006486.01.T01 MTR_5g034250 52.284 197 91 2 89 282 52 248 9.62e-71 219
MsG0280006486.01.T01 MTR_5g034250 52.284 197 91 2 89 282 74 270 1.95e-70 219
MsG0280006486.01.T01 MTR_8g064630 41.264 269 134 5 18 283 1 248 7.30e-69 215
MsG0280006486.01.T01 MTR_5g066060 48.347 242 110 6 7 239 10 245 5.18e-68 213
MsG0280006486.01.T01 MTR_6g006990 44.130 247 123 3 39 282 18 252 9.98e-68 212
MsG0280006486.01.T01 MTR_6g006990 44.130 247 123 3 39 282 89 323 2.87e-67 213
MsG0280006486.01.T01 MTR_3g077910 51.309 191 90 2 91 278 87 277 3.75e-62 199
MsG0280006486.01.T01 MTR_3g077940 49.738 191 93 2 91 278 55 245 1.62e-59 191
MsG0280006486.01.T01 MTR_8g064630 52.071 169 77 3 118 283 6 173 2.40e-58 185
MsG0280006486.01.T01 MTR_3g077940 51.724 174 81 2 108 278 33 206 1.26e-57 185
MsG0280006486.01.T01 MTR_6g006990 42.289 201 101 3 39 236 89 277 3.17e-46 157
MsG0280006486.01.T01 MTR_3g077930 42.000 200 90 4 93 286 8 187 1.14e-40 140
MsG0280006486.01.T01 MTR_5g066060 57.983 119 40 3 69 185 73 183 3.45e-39 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006486.01.T01 AT4G33580 58.824 272 100 5 37 302 36 301 4.00e-111 324
MsG0280006486.01.T01 AT4G33580 58.824 272 100 5 37 302 36 301 4.00e-111 324
MsG0280006486.01.T01 AT4G33580 58.242 273 101 6 37 302 36 302 1.21e-108 318
MsG0280006486.01.T01 AT1G58180 55.779 199 86 1 84 280 23 221 4.67e-82 248
MsG0280006486.01.T01 AT1G58180 55.779 199 86 1 84 280 23 221 4.67e-82 248
MsG0280006486.01.T01 AT1G58180 55.779 199 86 1 84 280 23 221 4.67e-82 248
MsG0280006486.01.T01 AT1G58180 55.779 199 86 1 84 280 74 272 7.72e-82 249
MsG0280006486.01.T01 AT1G58180 55.779 199 86 1 84 280 74 272 7.72e-82 249
MsG0280006486.01.T01 AT1G70410 49.767 215 104 2 75 285 38 252 8.20e-72 223
MsG0280006486.01.T01 AT1G70410 49.767 215 104 2 75 285 38 252 8.20e-72 223
MsG0280006486.01.T01 AT1G58180 55.618 178 77 1 84 259 74 251 1.70e-71 221
MsG0280006486.01.T01 AT1G70410 49.767 215 104 2 75 285 60 274 3.22e-71 222
MsG0280006486.01.T01 AT1G23730 48.848 217 107 2 73 285 36 252 8.00e-71 220
MsG0280006486.01.T01 AT1G23730 48.848 217 107 2 73 285 36 252 8.00e-71 220
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 50 251 9.08e-66 207
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 50 251 9.08e-66 207
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 50 251 9.08e-66 207
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 50 251 9.08e-66 207
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 50 251 9.08e-66 207
MsG0280006486.01.T01 AT5G14740 50.000 202 98 2 84 282 122 323 9.86e-65 207
MsG0280006486.01.T01 AT3G01500 48.515 202 101 2 84 282 50 251 4.33e-64 202
MsG0280006486.01.T01 AT3G01500 48.515 202 101 2 84 282 50 251 8.91e-64 202
MsG0280006486.01.T01 AT3G01500 48.515 202 101 2 84 282 127 328 2.24e-63 203
MsG0280006486.01.T01 AT3G01500 48.515 202 101 2 84 282 127 328 4.20e-63 203
MsG0280006486.01.T01 AT5G14740 49.505 202 98 3 84 282 122 322 3.02e-62 200
MsG0280006486.01.T01 AT5G14740 49.505 202 98 3 84 282 122 322 1.18e-61 199
MsG0280006486.01.T01 AT1G58180 57.419 155 64 1 84 236 74 228 5.75e-61 195
MsG0280006486.01.T01 AT5G14740 48.718 156 77 2 84 236 122 277 2.17e-43 151

Find 93 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATGCCCCTCCAATATTT+TGG 0.149705 2:-2633157 MsG0280006486.01.T01:CDS
AATCCGCAACGTTGCTAATT+TGG 0.153928 2:-2633109 MsG0280006486.01.T01:CDS
AAGTCAGTGTTCTTCTTTAA+AGG 0.218106 2:-2633649 MsG0280006486.01.T01:CDS
TTCTTCTTTAAAGGCTTCTA+TGG 0.218623 2:-2633640 MsG0280006486.01.T01:CDS
GCAAGACTTTCAAACTGTTC+AGG 0.276705 2:+2633386 None:intergenic
GAAATGGACATTGGACTATA+GGG 0.318375 2:-2631652 MsG0280006486.01.T01:CDS
ATCAAAAGTTGGGTTATTCA+TGG 0.325701 2:-2632043 MsG0280006486.01.T01:CDS
ATTTGCAGTAAACACTCTTC+TGG 0.333907 2:-2632665 MsG0280006486.01.T01:intron
CGTGTAGCTTTATCAAAAGT+TGG 0.339113 2:-2632054 MsG0280006486.01.T01:intron
AGAAATGGACATTGGACTAT+AGG 0.342791 2:-2631653 MsG0280006486.01.T01:CDS
TTAATTGTTCATTTGAGAAA+TGG 0.357926 2:-2631668 MsG0280006486.01.T01:CDS
TTATTGCCTGTGCAGATTCC+AGG 0.359167 2:-2633179 MsG0280006486.01.T01:CDS
CTCTGTTCCTGCGCAAATTA+CGG 0.359512 2:-2634414 MsG0280006486.01.T01:CDS
GTTGGTAAACAACATGGAAT+AGG 0.359886 2:+2634771 None:intergenic
AGACCCATTTGACAGCATTA+AGG 0.363149 2:-2634379 MsG0280006486.01.T01:intron
TACCCTTGATGGGTTATAAC+TGG 0.371016 2:+2634526 None:intergenic
TAATTTGGTTCCTACCTTTG+AGG 0.381517 2:-2633094 MsG0280006486.01.T01:intron
GGACTATAGGGGAACCAAAC+TGG 0.385882 2:-2631640 MsG0280006486.01.T01:CDS
GTGTCTAAAATTTATACCTC+TGG 0.386177 2:-2631531 MsG0280006486.01.T01:three_prime_UTR
TGGTTGAAATCCCAAAATAT+TGG 0.386230 2:+2633146 None:intergenic
TACACACACGTGAAATGGTT+GGG 0.395069 2:+2631431 None:intergenic
CAAGACTTTCAAACTGTTCA+GGG 0.397042 2:+2633387 None:intergenic
AAATCTACACACACGTGAAA+TGG 0.407565 2:+2631426 None:intergenic
GAATGAATGCATACCCTTGA+TGG 0.407802 2:+2634515 None:intergenic
CACACCTTAATGCTGTCAAA+TGG 0.409752 2:+2634375 None:intergenic
TTTGAGTTTGAGTTCTTCAA+TGG 0.413777 2:-2634606 MsG0280006486.01.T01:exon
GGAACCAAACTGGAGGAAAA+TGG 0.421310 2:-2631630 MsG0280006486.01.T01:CDS
ATCATCTTGCATACCCATAA+GGG 0.424264 2:+2632172 None:intergenic
CATCATCTTGCATACCCATA+AGG 0.436099 2:+2632171 None:intergenic
TGATCCAAGGGTAACTAAGT+AGG 0.442764 2:+2631745 None:intergenic
TACCTTTGGCGTTTGAACCT+TGG 0.443481 2:+2633364 None:intergenic
TAAACCTACTTAGTTACCCT+TGG 0.445262 2:-2631749 MsG0280006486.01.T01:CDS
TTATAACTGGAAGAGGATAA+AGG 0.445717 2:+2634539 None:intergenic
AACGAAGAACTCTCTATTCA+TGG 0.451547 2:-2631708 MsG0280006486.01.T01:CDS
TCGTTGGCCACAGCTGCTGT+GGG 0.452907 2:-2632205 MsG0280006486.01.T01:CDS
GTCGTTGGCCACAGCTGCTG+TGG 0.460065 2:-2632206 MsG0280006486.01.T01:CDS
GTGTAGCTTTATCAAAAGTT+GGG 0.461410 2:-2632053 MsG0280006486.01.T01:intron
GTGGATAAAGTTTGTAATGT+TGG 0.462582 2:+2634753 None:intergenic
GGTGGAAACTTTGTAGCAAA+GGG 0.463259 2:+2634560 None:intergenic
CACGAATTGGTAAGTCGATC+AGG 0.466661 2:+2631553 None:intergenic
ACACCTTAATGCTGTCAAAT+GGG 0.471844 2:+2634376 None:intergenic
TCTTCTTTAAAGGCTTCTAT+GGG 0.484512 2:-2633639 MsG0280006486.01.T01:CDS
TTGAGTTGCTGAGTATATCC+TGG 0.486112 2:+2633612 None:intergenic
TTCATTTGAGAAATGGACAT+TGG 0.487217 2:-2631661 MsG0280006486.01.T01:CDS
GAAAGAGTTATAAACATAGT+TGG 0.488329 2:+2634648 None:intergenic
CGGATTGTAAACGCATCTCC+TGG 0.489033 2:+2633126 None:intergenic
CTTCCAGTTATAACCCATCA+AGG 0.490907 2:-2634529 MsG0280006486.01.T01:CDS
TTACCCTTGGATCAAAGAAA+AGG 0.495682 2:-2631736 MsG0280006486.01.T01:CDS
TAATGCAGCATTTGTTTCTG+AGG 0.502192 2:+2632690 None:intergenic
TATTGCCTGTGCAGATTCCA+GGG 0.503483 2:-2633178 MsG0280006486.01.T01:CDS
AATGCAGCATTTGTTTCTGA+GGG 0.509806 2:+2632691 None:intergenic
AAGGCTTCTATGGGATCTCC+AGG 0.510919 2:-2633630 MsG0280006486.01.T01:CDS
GTAACTAAGTAGGTTTACTA+AGG 0.516928 2:+2631755 None:intergenic
AGGTGGAAACTTTGTAGCAA+AGG 0.522465 2:+2634559 None:intergenic
TGATGGGTTATAACTGGAAG+AGG 0.525265 2:+2634532 None:intergenic
TAAGTAGGTTTACTAAGGAA+TGG 0.532715 2:+2631760 None:intergenic
TGTCTAAAATTTATACCTCT+GGG 0.538011 2:-2631530 MsG0280006486.01.T01:three_prime_UTR
AAGGGATCAGAGATTGAAGA+AGG 0.543725 2:+2634578 None:intergenic
AATATTGGAGGGGCATACCC+TGG 0.554991 2:+2633161 None:intergenic
ACACACACGTGAAATGGTTG+GGG 0.562708 2:+2631432 None:intergenic
TGATATATTTAATGATCTGA+AGG 0.563309 2:-2633544 MsG0280006486.01.T01:CDS
CTACACACACGTGAAATGGT+TGG 0.563505 2:+2631430 None:intergenic
CTTATGGGTATGCAAGATGA+TGG 0.564663 2:-2632170 MsG0280006486.01.T01:CDS
AATGAATGCATACCCTTGAT+GGG 0.569966 2:+2634516 None:intergenic
TTGAAATCCCAAAATATTGG+AGG 0.570296 2:+2633149 None:intergenic
GAGGTGGGAAGCAGAAGCCT+TGG 0.571080 2:+2632000 None:intergenic
GTTGAGAACATCTTAGTCGT+TGG 0.572846 2:-2632221 MsG0280006486.01.T01:CDS
GTTGGCCACAGCTGCTGTGG+GGG 0.577076 2:-2632203 MsG0280006486.01.T01:CDS
CCCTTGGATCAAAGAAAAGG+TGG 0.580841 2:-2631733 MsG0280006486.01.T01:CDS
ACAGTTTGAAAGTCTTGCCA+AGG 0.582237 2:-2633381 MsG0280006486.01.T01:CDS
CCTTCTCGCAATGCGTACAC+TGG 0.586303 2:+2631966 None:intergenic
TGCAAGATGATGGCAGTACA+AGG 0.586853 2:-2632160 MsG0280006486.01.T01:intron
GCATACCCTGGAATCTGCAC+AGG 0.589047 2:+2633173 None:intergenic
TGAAATCCCAAAATATTGGA+GGG 0.589573 2:+2633150 None:intergenic
CGTTGGCCACAGCTGCTGTG+GGG 0.598268 2:-2632204 MsG0280006486.01.T01:CDS
CCAGTGTACGCATTGCGAGA+AGG 0.600622 2:-2631966 MsG0280006486.01.T01:intron
AAGGCCTATACCTCAAAGGT+AGG 0.606757 2:+2633084 None:intergenic
AAACAAGGCCTATACCTCAA+AGG 0.612041 2:+2633080 None:intergenic
TGTAATGTTGGTAAACAACA+TGG 0.613132 2:+2634765 None:intergenic
AAGTATAAAGAGTGTGTGTG+TGG 0.621516 2:+2634734 None:intergenic
GAAATCCCAAAATATTGGAG+GGG 0.631616 2:+2633151 None:intergenic
TGCCAAGGTTCAAACGCCAA+AGG 0.635821 2:-2633366 MsG0280006486.01.T01:intron
CTGGCGATCAAAGTCGAGGT+GGG 0.637149 2:+2631985 None:intergenic
TTCCAGTTATAACCCATCAA+GGG 0.637369 2:-2634528 MsG0280006486.01.T01:intron
AAATGGACATTGGACTATAG+GGG 0.637668 2:-2631651 MsG0280006486.01.T01:CDS
ACTTTAGCAGAAGTTAAAGA+CGG 0.637786 2:-2633573 MsG0280006486.01.T01:CDS
GAACCAAATTAGCAACGTTG+CGG 0.638872 2:+2633106 None:intergenic
ACTGGCGATCAAAGTCGAGG+TGG 0.654998 2:+2631984 None:intergenic
TAACTGGAAGAGGATAAAGG+TGG 0.660641 2:+2634542 None:intergenic
TACACTGGCGATCAAAGTCG+AGG 0.668978 2:+2631981 None:intergenic
AAATGCAAGAGTGAAAACCA+AGG 0.707710 2:-2632017 MsG0280006486.01.T01:CDS
CTATAGGGGAACCAAACTGG+AGG 0.709599 2:-2631637 MsG0280006486.01.T01:CDS
GAAGAACTCTCTATTCATGG+TGG 0.732574 2:-2631705 MsG0280006486.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAATCAAAAATTGA+GGG + Chr2:2631453-2631472 None:intergenic 10.0%
!!! TTAAATTGTTTTTATATTAC+AGG - Chr2:2634356-2634375 MsG0280006486.01.T01:intron 10.0%
!! AACAAAAAAGAACAAAAATT+TGG + Chr2:2631803-2631822 None:intergenic 15.0%
!! ACAAAAAAGAACAAAAATTT+GGG + Chr2:2631802-2631821 None:intergenic 15.0%
!! GATTAATTAATCAAAAATTG+AGG + Chr2:2631454-2631473 None:intergenic 15.0%
!! TTACAATTATAAAAGAATGT+TGG - Chr2:2633579-2633598 MsG0280006486.01.T01:CDS 15.0%
!!! ATTCTTTTAAAGTAAGAAAT+AGG - Chr2:2632375-2632394 MsG0280006486.01.T01:intron 15.0%
!!! CTACAAAAAATAAAGTATTT+TGG - Chr2:2634550-2634569 MsG0280006486.01.T01:CDS 15.0%
!!! TCGATTTTTTTTTTTAAACT+CGG + Chr2:2631951-2631970 None:intergenic 15.0%
!! AAAAAAAAAAATCGAGTCTT+CGG - Chr2:2631956-2631975 MsG0280006486.01.T01:intron 20.0%
!! ACAGAAATAACTTATATAAG+CGG + Chr2:2633144-2633163 None:intergenic 20.0%
!! ATTTATACCAAAGAAAGAAA+AGG + Chr2:2634039-2634058 None:intergenic 20.0%
!! TAAATTACAAGCTAATTCTA+TGG + Chr2:2632198-2632217 None:intergenic 20.0%
!! TAGGAACATTTAATTGAATT+AGG - Chr2:2632394-2632413 MsG0280006486.01.T01:intron 20.0%
!! TCATAAAGATTCTTCAAAAT+TGG + Chr2:2631909-2631928 None:intergenic 20.0%
!! TGATATATTTAATGATCTGA+AGG - Chr2:2632605-2632624 MsG0280006486.01.T01:intron 20.0%
!! TTAATTGTTCATTTGAGAAA+TGG - Chr2:2634481-2634500 MsG0280006486.01.T01:intron 20.0%
!! TTACATAATCAAGTTCTTAT+AGG + Chr2:2634314-2634333 None:intergenic 20.0%
!!! AAAAAATTGTTTTTCACCAT+TGG + Chr2:2632146-2632165 None:intergenic 20.0%
!!! CATTCTTTTATAATTGTAAC+AGG + Chr2:2633578-2633597 None:intergenic 20.0%
!!! TTTTGAAGAATCTTTATGAT+TGG - Chr2:2631910-2631929 MsG0280006486.01.T01:intron 20.0%
! AAAAAAAAAATCGAGTCTTC+GGG - Chr2:2631957-2631976 MsG0280006486.01.T01:intron 25.0%
! ACATTAGAACTTAGTTTAGA+AGG + Chr2:2632471-2632490 None:intergenic 25.0%
! ATAAATCAACAAACAGCTAA+AGG + Chr2:2632852-2632871 None:intergenic 25.0%
! ATTGAGTGATATGAACTATT+AGG - Chr2:2634262-2634281 MsG0280006486.01.T01:intron 25.0%
! GAAAATAGAAGTAAAAACTC+AGG + Chr2:2634577-2634596 None:intergenic 25.0%
! GAAAGAGTTATAAACATAGT+TGG + Chr2:2631504-2631523 None:intergenic 25.0%
! GAATTGAAGAATGAACTTAA+AGG + Chr2:2632890-2632909 None:intergenic 25.0%
! TATTAAGTTTGAATCTTCAC+CGG - Chr2:2631875-2631894 MsG0280006486.01.T01:intron 25.0%
! TCAAATAAGTTATTCGTAAC+AGG - Chr2:2633322-2633341 MsG0280006486.01.T01:intron 25.0%
! TGAAATCAACCAATAAGATA+AGG - Chr2:2633758-2633777 MsG0280006486.01.T01:intron 25.0%
! TGCTTACTTTATGTTAATTG+AGG - Chr2:2631839-2631858 MsG0280006486.01.T01:intron 25.0%
!! AAAAATAATGTTTCTGACCA+TGG + Chr2:2632681-2632700 None:intergenic 25.0%
!! AACTAAGTTCTAATGTTTGT+AGG - Chr2:2632475-2632494 MsG0280006486.01.T01:intron 25.0%
!! AAGATTTTCACTTCTTGTTA+AGG - Chr2:2633883-2633902 MsG0280006486.01.T01:intron 25.0%
!! AATACTTTGAAAACAGCTTA+TGG - Chr2:2633238-2633257 MsG0280006486.01.T01:intron 25.0%
!! AGGTACTTAATTCTAGTTAT+CGG - Chr2:2634009-2634028 MsG0280006486.01.T01:intron 25.0%
!! AGTTGTTATTATCATTGCTA+TGG - Chr2:2633374-2633393 MsG0280006486.01.T01:CDS 25.0%
!! TAAATTTTAGACACACGAAT+TGG + Chr2:2634612-2634631 None:intergenic 25.0%
!! TGTCTAAAATTTATACCTCT+GGG - Chr2:2634619-2634638 MsG0280006486.01.T01:five_prime_UTR 25.0%
!!! GTGAAAAACAATTTTTTGCA+TGG - Chr2:2632149-2632168 MsG0280006486.01.T01:intron 25.0%
!!! TCTATTAGCTTTCTTAATAG+TGG - Chr2:2633848-2633867 MsG0280006486.01.T01:intron 25.0%
!!! TTAGTTCTGTTTTATTGTCA+GGG - Chr2:2632735-2632754 MsG0280006486.01.T01:intron 25.0%
!!! TTGAAATCTCTTTTTTTCTG+TGG - Chr2:2632439-2632458 MsG0280006486.01.T01:intron 25.0%
!!! TTTAGCTGTTTGTTGATTTA+TGG - Chr2:2632851-2632870 MsG0280006486.01.T01:intron 25.0%
AAAAGAATAGCTGCGAAAAA+TGG + Chr2:2632363-2632382 None:intergenic 30.0%
AAAGACTGAACAATTACCTT+TGG + Chr2:2632802-2632821 None:intergenic 30.0%
AAGTCAGTGTTCTTCTTTAA+AGG - Chr2:2632500-2632519 MsG0280006486.01.T01:intron 30.0%
AATGTGTAAATTCACTGTTG+AGG - Chr2:2634230-2634249 MsG0280006486.01.T01:intron 30.0%
ACTTTAGCAGAAGTTAAAGA+CGG - Chr2:2632576-2632595 MsG0280006486.01.T01:intron 30.0%
AGAAAACAGAGTAAAAACAG+AGG + Chr2:2631659-2631678 None:intergenic 30.0%
AGAATTAGAAGAAGCAAGTA+GGG + Chr2:2633417-2633436 None:intergenic 30.0%
ATCAAAAGTTGGGTTATTCA+TGG - Chr2:2634106-2634125 MsG0280006486.01.T01:intron 30.0%
ATCAAATCAAATAGTGTCTG+TGG - Chr2:2632281-2632300 MsG0280006486.01.T01:intron 30.0%
ATGAAAAACAGACTTGTGTA+TGG + Chr2:2632066-2632085 None:intergenic 30.0%
ATGTCATGACCTTATCTTAT+TGG + Chr2:2633770-2633789 None:intergenic 30.0%
ATTGAATTAGGCTTACTGAA+AGG - Chr2:2632406-2632425 MsG0280006486.01.T01:intron 30.0%
CAACAATTTCTATAGAAAGG+TGG - Chr2:2633632-2633651 MsG0280006486.01.T01:CDS 30.0%
CGAAGTTGTTTATTCAAACA+AGG + Chr2:2633087-2633106 None:intergenic 30.0%
CTGCAACAATTTCTATAGAA+AGG - Chr2:2633629-2633648 MsG0280006486.01.T01:CDS 30.0%
GTAACTAAGTAGGTTTACTA+AGG + Chr2:2634397-2634416 None:intergenic 30.0%
GTGGATAAAGTTTGTAATGT+TGG + Chr2:2631399-2631418 None:intergenic 30.0%
TAAGTAGGTTTACTAAGGAA+TGG + Chr2:2634392-2634411 None:intergenic 30.0%
TCTTTGTGATCTAAATGTGT+TGG - Chr2:2632311-2632330 MsG0280006486.01.T01:intron 30.0%
TGAAATCCCAAAATATTGGA+GGG + Chr2:2633002-2633021 None:intergenic 30.0%
TGGTTGAAATCCCAAAATAT+TGG + Chr2:2633006-2633025 None:intergenic 30.0%
TTATAACTGGAAGAGGATAA+AGG + Chr2:2631613-2631632 None:intergenic 30.0%
TTCATTTGAGAAATGGACAT+TGG - Chr2:2634488-2634507 MsG0280006486.01.T01:intron 30.0%
TTGAAATCCCAAAATATTGG+AGG + Chr2:2633003-2633022 None:intergenic 30.0%
! AAACAGAGTTTTTATGTCAG+TGG - Chr2:2633293-2633312 MsG0280006486.01.T01:intron 30.0%
! ACTGTTTTCATAAGCTATAC+TGG - Chr2:2633203-2633222 MsG0280006486.01.T01:intron 30.0%
! CAACTTTTGATAAAGCTACA+CGG + Chr2:2634097-2634116 None:intergenic 30.0%
! CCATGATGAAATTTTTCTCT+TGG - Chr2:2634781-2634800 MsG0280006486.01.T01:five_prime_UTR 30.0%
! GTGTCTAAAATTTATACCTC+TGG - Chr2:2634618-2634637 MsG0280006486.01.T01:five_prime_UTR 30.0%
! TCTTCTTTAAAGGCTTCTAT+GGG - Chr2:2632510-2632529 MsG0280006486.01.T01:intron 30.0%
! TGTAATGTTGGTAAACAACA+TGG + Chr2:2631387-2631406 None:intergenic 30.0%
! TTCTTCTTTAAAGGCTTCTA+TGG - Chr2:2632509-2632528 MsG0280006486.01.T01:intron 30.0%
! TTTGAGTTTGAGTTCTTCAA+TGG - Chr2:2631543-2631562 MsG0280006486.01.T01:three_prime_UTR 30.0%
!! ACAAGTCTGTTTTTCATCAT+TGG - Chr2:2632069-2632088 MsG0280006486.01.T01:intron 30.0%
!! GTGTAGCTTTATCAAAAGTT+GGG - Chr2:2634096-2634115 MsG0280006486.01.T01:intron 30.0%
!!! AATATTTTGGGATTTCAACC+AGG - Chr2:2633005-2633024 MsG0280006486.01.T01:intron 30.0%
!!! ATAGACATTTTTTTTCCTGC+AGG - Chr2:2633906-2633925 MsG0280006486.01.T01:intron 30.0%
!!! GTTAATTGAGGTGTTTTTAG+TGG - Chr2:2631851-2631870 MsG0280006486.01.T01:intron 30.0%
!!! GTTAGTTCTGTTTTATTGTC+AGG - Chr2:2632734-2632753 MsG0280006486.01.T01:intron 30.0%
AAATCTACACACACGTGAAA+TGG + Chr2:2634726-2634745 None:intergenic 35.0%
AAATGCAAGAGTGAAAACCA+AGG - Chr2:2634132-2634151 MsG0280006486.01.T01:intron 35.0%
AAATGGACATTGGACTATAG+GGG - Chr2:2634498-2634517 MsG0280006486.01.T01:intron 35.0%
AACGAAGAACTCTCTATTCA+TGG - Chr2:2634441-2634460 MsG0280006486.01.T01:intron 35.0%
AATCATGTCTTGCACTATGT+AGG + Chr2:2633525-2633544 None:intergenic 35.0%
AATGAATGCATACCCTTGAT+GGG + Chr2:2631636-2631655 None:intergenic 35.0%
ACACCTTAATGCTGTCAAAT+GGG + Chr2:2631776-2631795 None:intergenic 35.0%
ACCATTGGATCAATAAGTCT+CGG + Chr2:2632131-2632150 None:intergenic 35.0%
AGAAATGGACATTGGACTAT+AGG - Chr2:2634496-2634515 MsG0280006486.01.T01:intron 35.0%
ATCATCTTGCATACCCATAA+GGG + Chr2:2633980-2633999 None:intergenic 35.0%
ATTTGCAGTAAACACTCTTC+TGG - Chr2:2633484-2633503 MsG0280006486.01.T01:intron 35.0%
CAAGACTTTCAAACTGTTCA+GGG + Chr2:2632765-2632784 None:intergenic 35.0%
CAATAACCATGAACTGTTCT+TGG + Chr2:2632956-2632975 None:intergenic 35.0%
GAAAGGTGGAATTTAAAGAG+CGG - Chr2:2633646-2633665 MsG0280006486.01.T01:CDS 35.0%
GAAATCCCAAAATATTGGAG+GGG + Chr2:2633001-2633020 None:intergenic 35.0%
GAAATGGACATTGGACTATA+GGG - Chr2:2634497-2634516 MsG0280006486.01.T01:intron 35.0%
GAGAATTAGAAGAAGCAAGT+AGG + Chr2:2633418-2633437 None:intergenic 35.0%
GATTCTTCAAAATTGGTCGT+CGG + Chr2:2631902-2631921 None:intergenic 35.0%
GCATACTGATATTGTAACTC+AGG - Chr2:2632004-2632023 MsG0280006486.01.T01:CDS 35.0%
GTCAATAGAAGTGAAACTTC+AGG + Chr2:2633348-2633367 None:intergenic 35.0%
GTCTTGGATCAAGTGATATA+TGG + Chr2:2632109-2632128 None:intergenic 35.0%
TAAACCTACTTAGTTACCCT+TGG - Chr2:2634400-2634419 MsG0280006486.01.T01:CDS 35.0%
TATGGAAAGTTAAGCTTCGA+GGG - Chr2:2633671-2633690 MsG0280006486.01.T01:intron 35.0%
TGTAAGCTTCAGAAAATCCA+TGG - Chr2:2632661-2632680 MsG0280006486.01.T01:intron 35.0%
TTACCCTTGGATCAAAGAAA+AGG - Chr2:2634413-2634432 MsG0280006486.01.T01:CDS 35.0%
TTCCAGTTATAACCCATCAA+GGG - Chr2:2631621-2631640 MsG0280006486.01.T01:CDS 35.0%
! AAGTATAAAGAGTGTGTGTG+TGG + Chr2:2631418-2631437 None:intergenic 35.0%
! ACTTTTCCAAGAACAGTTCA+TGG - Chr2:2632947-2632966 MsG0280006486.01.T01:intron 35.0%
! CGGTTTTCCTTTTCTTTCTT+TGG - Chr2:2634029-2634048 MsG0280006486.01.T01:intron 35.0%
! GAATCTTTATGATTGGTCGT+CGG - Chr2:2631917-2631936 MsG0280006486.01.T01:intron 35.0%
! GTTGGTAAACAACATGGAAT+AGG + Chr2:2631381-2631400 None:intergenic 35.0%
! TAATTTGGTTCCTACCTTTG+AGG - Chr2:2633055-2633074 MsG0280006486.01.T01:intron 35.0%
!! AATGCAGCATTTGTTTCTGA+GGG + Chr2:2633461-2633480 None:intergenic 35.0%
!! CGTGTAGCTTTATCAAAAGT+TGG - Chr2:2634095-2634114 MsG0280006486.01.T01:intron 35.0%
!! TAATGCAGCATTTGTTTCTG+AGG + Chr2:2633462-2633481 None:intergenic 35.0%
AAACAAGGCCTATACCTCAA+AGG + Chr2:2633072-2633091 None:intergenic 40.0%
AAGCTATACTGGAGAGCTTA+TGG - Chr2:2633214-2633233 MsG0280006486.01.T01:intron 40.0%
AAGGGATCAGAGATTGAAGA+AGG + Chr2:2631574-2631593 None:intergenic 40.0%
AATCCGCAACGTTGCTAATT+TGG - Chr2:2633040-2633059 MsG0280006486.01.T01:intron 40.0%
ACAGTTTGAAAGTCTTGCCA+AGG - Chr2:2632768-2632787 MsG0280006486.01.T01:intron 40.0%
ACCGAGACTTATTGATCCAA+TGG - Chr2:2632127-2632146 MsG0280006486.01.T01:intron 40.0%
AGACCCATTTGACAGCATTA+AGG - Chr2:2631770-2631789 MsG0280006486.01.T01:CDS 40.0%
AGGTGGAAACTTTGTAGCAA+AGG + Chr2:2631593-2631612 None:intergenic 40.0%
CACACCTTAATGCTGTCAAA+TGG + Chr2:2631777-2631796 None:intergenic 40.0%
CATCATCTTGCATACCCATA+AGG + Chr2:2633981-2634000 None:intergenic 40.0%
CTATCTCACTTGTACACCAT+AGG + Chr2:2632035-2632054 None:intergenic 40.0%
CTATGGTGTACAAGTGAGAT+AGG - Chr2:2632033-2632052 MsG0280006486.01.T01:CDS 40.0%
CTTATGGGTATGCAAGATGA+TGG - Chr2:2633979-2633998 MsG0280006486.01.T01:intron 40.0%
CTTCCAGTTATAACCCATCA+AGG - Chr2:2631620-2631639 MsG0280006486.01.T01:CDS 40.0%
GAACCAAATTAGCAACGTTG+CGG + Chr2:2633046-2633065 None:intergenic 40.0%
GAAGAACTCTCTATTCATGG+TGG - Chr2:2634444-2634463 MsG0280006486.01.T01:intron 40.0%
GAATGAATGCATACCCTTGA+TGG + Chr2:2631637-2631656 None:intergenic 40.0%
GCAAGACTTTCAAACTGTTC+AGG + Chr2:2632766-2632785 None:intergenic 40.0%
GGAATTTAAAGAGCGGAGTA+TGG - Chr2:2633653-2633672 MsG0280006486.01.T01:CDS 40.0%
GGTGGAAACTTTGTAGCAAA+GGG + Chr2:2631592-2631611 None:intergenic 40.0%
GTATGGAAAGTTAAGCTTCG+AGG - Chr2:2633670-2633689 MsG0280006486.01.T01:intron 40.0%
GTTGAGAACATCTTAGTCGT+TGG - Chr2:2633928-2633947 MsG0280006486.01.T01:intron 40.0%
TAACTGGAAGAGGATAAAGG+TGG + Chr2:2631610-2631629 None:intergenic 40.0%
TACACACACGTGAAATGGTT+GGG + Chr2:2634721-2634740 None:intergenic 40.0%
TCTCTCTATTATCCACCTTC+TGG - Chr2:2632821-2632840 MsG0280006486.01.T01:intron 40.0%
TGATCCAAGGGTAACTAAGT+AGG + Chr2:2634407-2634426 None:intergenic 40.0%
! ATCTATGCTTTTAGTCCCAG+AGG + Chr2:2634637-2634656 None:intergenic 40.0%
! CCACCTTTTCTTTGATCCAA+GGG + Chr2:2634419-2634438 None:intergenic 40.0%
! GTATGCCCCTCCAATATTTT+GGG - Chr2:2632993-2633012 MsG0280006486.01.T01:intron 40.0%
! TCCACCTTTTCTTTGATCCA+AGG + Chr2:2634420-2634439 None:intergenic 40.0%
! TCTCTTTTCCTGATACAGAG+TGG - Chr2:2633434-2633453 MsG0280006486.01.T01:intron 40.0%
! TGATCTAAATGTGTTGGCAC+TGG - Chr2:2632317-2632336 MsG0280006486.01.T01:intron 40.0%
! TTGAGTTGCTGAGTATATCC+TGG + Chr2:2632540-2632559 None:intergenic 40.0%
! TTTATGATTGGTCGTCGGTT+TGG - Chr2:2631922-2631941 MsG0280006486.01.T01:intron 40.0%
!! ATTTTAGCCGTAATTTGCGC+AGG + Chr2:2631745-2631764 None:intergenic 40.0%
!! TACCCTTGATGGGTTATAAC+TGG + Chr2:2631626-2631645 None:intergenic 40.0%
!! TGATGGGTTATAACTGGAAG+AGG + Chr2:2631620-2631639 None:intergenic 40.0%
AAGGCCTATACCTCAAAGGT+AGG + Chr2:2633068-2633087 None:intergenic 45.0%
ACACACACGTGAAATGGTTG+GGG + Chr2:2634720-2634739 None:intergenic 45.0%
CACGAATTGGTAAGTCGATC+AGG + Chr2:2634599-2634618 None:intergenic 45.0%
CCCTTGGATCAAAGAAAAGG+TGG - Chr2:2634416-2634435 MsG0280006486.01.T01:CDS 45.0%
CTAAGATGTTCTCAACCTGC+AGG + Chr2:2633924-2633943 None:intergenic 45.0%
CTACACACACGTGAAATGGT+TGG + Chr2:2634722-2634741 None:intergenic 45.0%
CTCTGTTCCTGCGCAAATTA+CGG - Chr2:2631735-2631754 MsG0280006486.01.T01:CDS 45.0%
GGAACCAAACTGGAGGAAAA+TGG - Chr2:2634519-2634538 MsG0280006486.01.T01:intron 45.0%
GGATCAATAAGTCTCGGTCT+TGG + Chr2:2632125-2632144 None:intergenic 45.0%
TACCTTTGGCGTTTGAACCT+TGG + Chr2:2632788-2632807 None:intergenic 45.0%
TATTGCCTGTGCAGATTCCA+GGG - Chr2:2632971-2632990 MsG0280006486.01.T01:intron 45.0%
TTATTGCCTGTGCAGATTCC+AGG - Chr2:2632970-2632989 MsG0280006486.01.T01:intron 45.0%
TTCAAAATTGGTCGTCGGTC+CGG + Chr2:2631897-2631916 None:intergenic 45.0%
! GGTATGCCCCTCCAATATTT+TGG - Chr2:2632992-2633011 MsG0280006486.01.T01:intron 45.0%
! TGCAAGATGATGGCAGTACA+AGG - Chr2:2633989-2634008 MsG0280006486.01.T01:intron 45.0%
! TGTAACTCAGGCTAGTCCTA+TGG - Chr2:2632016-2632035 MsG0280006486.01.T01:CDS 45.0%
!! GGTTCCTACCTTTGAGGTAT+AGG - Chr2:2633061-2633080 MsG0280006486.01.T01:intron 45.0%
!!! TCTGCCATTTTCCTCCAGTT+TGG + Chr2:2634526-2634545 None:intergenic 45.0%
AAGGCTTCTATGGGATCTCC+AGG - Chr2:2632519-2632538 MsG0280006486.01.T01:intron 50.0%
CAGCTAAAGGCTTAGCCAGA+AGG + Chr2:2632839-2632858 None:intergenic 50.0%
CGGATTGTAAACGCATCTCC+TGG + Chr2:2633026-2633045 None:intergenic 50.0%
CTAAAGGCTTAGCCAGAAGG+TGG + Chr2:2632836-2632855 None:intergenic 50.0%
CTATAGGGGAACCAAACTGG+AGG - Chr2:2634512-2634531 MsG0280006486.01.T01:intron 50.0%
GGACTATAGGGGAACCAAAC+TGG - Chr2:2634509-2634528 MsG0280006486.01.T01:intron 50.0%
TACACTGGCGATCAAAGTCG+AGG + Chr2:2634171-2634190 None:intergenic 50.0%
TGCCAAGGTTCAAACGCCAA+AGG - Chr2:2632783-2632802 MsG0280006486.01.T01:intron 50.0%
! AATATTGGAGGGGCATACCC+TGG + Chr2:2632991-2633010 None:intergenic 50.0%
ACTGGCGATCAAAGTCGAGG+TGG + Chr2:2634168-2634187 None:intergenic 55.0%
CCAGTGTACGCATTGCGAGA+AGG - Chr2:2634183-2634202 MsG0280006486.01.T01:intron 55.0%
CCTTCTCGCAATGCGTACAC+TGG + Chr2:2634186-2634205 None:intergenic 55.0%
CTGAGGGTCCACTCTGTATC+AGG + Chr2:2633445-2633464 None:intergenic 55.0%
CTGGCGATCAAAGTCGAGGT+GGG + Chr2:2634167-2634186 None:intergenic 55.0%
GCATACCCTGGAATCTGCAC+AGG + Chr2:2632979-2632998 None:intergenic 55.0%
GAGGTGGGAAGCAGAAGCCT+TGG + Chr2:2634152-2634171 None:intergenic 60.0%
GTATACCCCCACAGCAGCTG+TGG + Chr2:2633954-2633973 None:intergenic 60.0%
TCGTTGGCCACAGCTGCTGT+GGG - Chr2:2633944-2633963 MsG0280006486.01.T01:intron 60.0%
! GGGGGTATACGCGCCCTTAT+GGG - Chr2:2633964-2633983 MsG0280006486.01.T01:intron 60.0%
! TGGGGGTATACGCGCCCTTA+TGG - Chr2:2633963-2633982 MsG0280006486.01.T01:intron 60.0%
CGTTGGCCACAGCTGCTGTG+GGG - Chr2:2633945-2633964 MsG0280006486.01.T01:intron 65.0%
GTCGTTGGCCACAGCTGCTG+TGG - Chr2:2633943-2633962 MsG0280006486.01.T01:intron 65.0%
GTTGGCCACAGCTGCTGTGG+GGG - Chr2:2633946-2633965 MsG0280006486.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 2631370 2634801 2631370 ID=MsG0280006486.01;Name=MsG0280006486.01
Chr2 mRNA 2631370 2634801 2631370 ID=MsG0280006486.01.T01;Parent=MsG0280006486.01;Name=MsG0280006486.01.T01;_AED=0.32;_eAED=0.32;_QI=192|1|1|1|0.75|0.88|9|223|302
Chr2 exon 2631370 2631793 2631370 ID=MsG0280006486.01.T01:exon:505;Parent=MsG0280006486.01.T01
Chr2 exon 2631967 2632069 2631967 ID=MsG0280006486.01.T01:exon:504;Parent=MsG0280006486.01.T01
Chr2 exon 2632161 2632243 2632161 ID=MsG0280006486.01.T01:exon:503;Parent=MsG0280006486.01.T01
Chr2 exon 2632666 2632719 2632666 ID=MsG0280006486.01.T01:exon:502;Parent=MsG0280006486.01.T01
Chr2 exon 2633095 2633208 2633095 ID=MsG0280006486.01.T01:exon:501;Parent=MsG0280006486.01.T01
Chr2 exon 2633367 2633415 2633367 ID=MsG0280006486.01.T01:exon:500;Parent=MsG0280006486.01.T01
Chr2 exon 2633510 2633674 2633510 ID=MsG0280006486.01.T01:exon:499;Parent=MsG0280006486.01.T01
Chr2 exon 2634380 2634438 2634380 ID=MsG0280006486.01.T01:exon:498;Parent=MsG0280006486.01.T01
Chr2 exon 2634529 2634801 2634529 ID=MsG0280006486.01.T01:exon:497;Parent=MsG0280006486.01.T01
Chr2 five_prime_UTR 2634610 2634801 2634610 ID=MsG0280006486.01.T01:five_prime_utr;Parent=MsG0280006486.01.T01
Chr2 CDS 2634529 2634609 2634529 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2634380 2634438 2634380 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2633510 2633674 2633510 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2633367 2633415 2633367 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2633095 2633208 2633095 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2632666 2632719 2632666 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2632161 2632243 2632161 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2631967 2632069 2631967 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 CDS 2631593 2631793 2631593 ID=MsG0280006486.01.T01:cds;Parent=MsG0280006486.01.T01
Chr2 three_prime_UTR 2631370 2631592 2631370 ID=MsG0280006486.01.T01:three_prime_utr;Parent=MsG0280006486.01.T01
Gene Sequence

>MsG0280006486.01.T01

ATGGCAATTCCTTCTTCAATCTCTGATCCCTTTGCTACAAAGTTTCCACCTTTATCCTCTTCCAGTTATAACCCATCAAGGATCTCTGTTCCTGCGCAAATTACGGCTAAAATTGAGCAGACCCATTTGACAGCATTAAGGAGAAGTCAGTGTTCTTCTTTAAAGGCTTCTATGGGATCTCCAGGATATACTCAGCAACTCAATAACAGCAAGTTAGATACTTTAGCAGAAGTTAAAGACGGACGTGATGATATATTTAATGATCTGAAGGATAGATTTTTAAGTTTTAAGAAGAATGTATACATGGAAAACCCTGAACAGTTTGAAAGTCTTGCCAAGGTTCAAACGCCAAAGTTCATGGTTATTGCCTGTGCAGATTCCAGGGTATGCCCCTCCAATATTTTGGGATTTCAACCAGGAGATGCGTTTACAATCCGCAACGTTGCTAATTTGGTTCCTACCTTTGAGAGTGGACCCTCAGAAACAAATGCTGCATTAGAATTTGCAGTAAACACTCTTCTGGTTGAGAACATCTTAGTCGTTGGCCACAGCTGCTGTGGGGGTATACGCGCCCTTATGGGTATGCAAGATGATGGCAGTACAAGCTTTATCAAAAGTTGGGTTATTCATGGAAAAAATGCAAGAGTGAAAACCAAGGCTTCTGCTTCCCACCTCGACTTTGATCGCCAGTGTACGCATTGCGAGAAGGAATCAATTAACCATTCCTTAGTAAACCTACTTAGTTACCCTTGGATCAAAGAAAAGGTGGAGAACGAAGAACTCTCTATTCATGGTGGTTACTATGACTTTGTTAATTGTTCATTTGAGAAATGGACATTGGACTATAGGGGAACCAAACTGGAGGAAAATGGCAGAATTGCTACAAAAAATAAAGTATTTTGGTCCTGA
Protein sequence

>MsG0280006486.01.T01

MAIPSSISDPFATKFPPLSSSSYNPSRISVPAQITAKIEQTHLTALRRSQCSSLKASMGSPGYTQQLNNSKLDTLAEVKDGRDDIFNDLKDRFLSFKKNVYMENPEQFESLAKVQTPKFMVIACADSRVCPSNILGFQPGDAFTIRNVANLVPTFESGPSETNAALEFAVNTLLVENILVVGHSCCGGIRALMGMQDDGSTSFIKSWVIHGKNARVKTKASASHLDFDRQCTHCEKESINHSLVNLLSYPWIKEKVENEELSIHGGYYDFVNCSFEKWTLDYRGTKLEENGRIATKNKVFWS*