AlfalfaGEDB | MsG0480023752.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023752.01

KEGG: K01214
GO: GO:0003824 GO:0004553 GO:0005975
PFAM: PF00128 PF02922

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023752.01.T01	XP_003630623.2	90.99	788	46	5	3	765	1	788	0	1464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023752.01.T01	O04196	72.335	741	176	6	48	761	41	779	0	1117

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023752.01.T01	G7LIX1	90.990	788	46	5	3	765	1	788	0.0	1464

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048776.01	MsG0480023752.01	0.816770	4.658411e-52	3.331015e-49
MsG0080048900.01	MsG0480023752.01	0.826779	2.249003e-54	2.129699e-51
MsG0080048992.01	MsG0480023752.01	0.803633	3.175644e-49	1.603854e-46
MsG0080049016.01	MsG0480023752.01	0.832827	7.567220e-56	8.556651e-53
MsG0080049018.01	MsG0480023752.01	0.804043	2.610337e-49	1.332336e-46
MsG0180002902.01	MsG0480023752.01	0.805001	1.648107e-49	8.621877e-47
MsG0180002982.01	MsG0480023752.01	0.852424	4.657714e-61	9.727724e-58
MsG0180003156.01	MsG0480023752.01	0.800336	1.511978e-48	7.022030e-46
MsG0180003268.01	MsG0480023752.01	0.818743	1.671115e-52	1.261463e-49
MsG0180004193.01	MsG0480023752.01	0.802462	5.546362e-49	2.718564e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023752.01.T01	MTR_8g101490	90.990	788	46	5	3	765	1	788	0.0	1464
MsG0480023752.01.T01	MTR_8g101490	89.308	636	39	5	3	611	1	634	0.0	1149
MsG0480023752.01.T01	MTR_3g099500	42.201	718	373	19	57	757	81	773	2.80e-173	517
MsG0480023752.01.T01	MTR_3g099500	41.224	735	373	20	57	757	81	790	3.49e-169	507
MsG0480023752.01.T01	MTR_7g094560	31.953	676	379	25	75	725	202	821	1.81e-82	281

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023752.01.T01	AT2G39930	72.335	741	176	6	48	761	41	779	0.0	1117
MsG0480023752.01.T01	AT2G39930	72.213	619	143	6	48	639	41	657	0.0	930
MsG0480023752.01.T01	AT2G39930	70.567	564	137	6	48	584	41	602	0.0	820
MsG0480023752.01.T01	AT4G09020	42.685	745	380	21	34	757	43	761	2.62e-179	532
MsG0480023752.01.T01	AT1G03310	29.213	712	412	22	74	757	228	875	4.23e-79	272
MsG0480023752.01.T01	AT1G03310	29.213	712	412	22	74	757	228	875	4.23e-79	272

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Find 210 sgRNAs with CRISPR-Local

Find 710 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAACTCAGATGGACT+CGG	0.579909	4:-89389670	MsG0480023752.01.T01:intron
AAAATTTGTGATAAATTGTG+CGG	0.593445	4:+89389364	None:intergenic
AAAGGCTACCCTTCACCCTA+CGG	0.576668	4:-89399541	MsG0480023752.01.T01:CDS
AAATCTGAAGAAGTCTGACG+AGG	0.720742	4:+89390864	None:intergenic
AAATGTAATGAGAGTGTGTG+TGG	0.673976	4:+89399818	None:intergenic
AAATTGACCCCGTCTTCTTG+AGG	0.573022	4:+89399511	None:intergenic
AACACCGACACGATTCCCAA+CGG	0.668297	4:+89399615	None:intergenic
AACCTTGTATGTGCTCATGA+TGG	0.532079	4:-89392626	MsG0480023752.01.T01:CDS
AACTCAGATGGACTCGGTGA+AGG	0.542854	4:-89389664	MsG0480023752.01.T01:intron
AACTCCTCTAAAAGAAATAA+TGG	0.265535	4:+89396973	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGGAATATAAGAGTTC+TGG	-	Chr4:89398237-89398256	MsG0480023752.01.T01:intron	30.0%
AACCAGTTAATAGTGATCAA+TGG	-	Chr4:89392088-89392107	MsG0480023752.01.T01:intron	30.0%
AACTCAAATCTCAAAGAAGT+AGG	-	Chr4:89391991-89392010	MsG0480023752.01.T01:intron	30.0%
AAGAATGTTTAGGAATACCT+TGG	+	Chr4:89391925-89391944	None:intergenic	30.0%
AAGATAATGTGTCCTCTAAA+AGG	-	Chr4:89390390-89390409	MsG0480023752.01.T01:intron	30.0%
AAGTTCATCTAATTTAGCAC+TGG	+	Chr4:89395194-89395213	None:intergenic	30.0%
AATTGTGATAAAATCCTCAG+TGG	-	Chr4:89390532-89390551	MsG0480023752.01.T01:intron	30.0%
ACAAGGTTAATACTATTCCA+TGG	+	Chr4:89396206-89396225	None:intergenic	30.0%
ACTCTTATATTCCTGTTTCA+TGG	+	Chr4:89398235-89398254	None:intergenic	30.0%
AGAATGTTTAGGAATACCTT+GGG	+	Chr4:89391924-89391943	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	89388957	89399909	89388957	ID=MsG0480023752.01;Name=MsG0480023752.01
Chr4	mRNA	89388957	89399909	89388957	ID=MsG0480023752.01.T01;Parent=MsG0480023752.01;Name=MsG0480023752.01.T01;_AED=0.49;_eAED=0.51;_QI=124\|0.88\|0.94\|1\|0.94\|0.88\|18\|174\|765
Chr4	exon	89388957	89388980	89388957	ID=MsG0480023752.01.T01:exon:7831;Parent=MsG0480023752.01.T01
Chr4	exon	89389266	89389679	89389266	ID=MsG0480023752.01.T01:exon:7830;Parent=MsG0480023752.01.T01
Chr4	exon	89390651	89390768	89390651	ID=MsG0480023752.01.T01:exon:7829;Parent=MsG0480023752.01.T01
Chr4	exon	89390838	89390920	89390838	ID=MsG0480023752.01.T01:exon:7828;Parent=MsG0480023752.01.T01
Chr4	exon	89391005	89391085	89391005	ID=MsG0480023752.01.T01:exon:7827;Parent=MsG0480023752.01.T01
Chr4	exon	89391739	89391822	89391739	ID=MsG0480023752.01.T01:exon:7826;Parent=MsG0480023752.01.T01
Chr4	exon	89392520	89392675	89392520	ID=MsG0480023752.01.T01:exon:7825;Parent=MsG0480023752.01.T01
Chr4	exon	89392961	89393053	89392961	ID=MsG0480023752.01.T01:exon:7824;Parent=MsG0480023752.01.T01
Chr4	exon	89393982	89394068	89393982	ID=MsG0480023752.01.T01:exon:7823;Parent=MsG0480023752.01.T01
Chr4	exon	89395097	89395223	89395097	ID=MsG0480023752.01.T01:exon:7822;Parent=MsG0480023752.01.T01
Chr4	exon	89395379	89395470	89395379	ID=MsG0480023752.01.T01:exon:7821;Parent=MsG0480023752.01.T01
Chr4	exon	89396940	89397047	89396940	ID=MsG0480023752.01.T01:exon:7820;Parent=MsG0480023752.01.T01
Chr4	exon	89397144	89397285	89397144	ID=MsG0480023752.01.T01:exon:7819;Parent=MsG0480023752.01.T01
Chr4	exon	89397810	89397901	89397810	ID=MsG0480023752.01.T01:exon:7818;Parent=MsG0480023752.01.T01
Chr4	exon	89398580	89398732	89398580	ID=MsG0480023752.01.T01:exon:7817;Parent=MsG0480023752.01.T01
Chr4	exon	89398840	89398932	89398840	ID=MsG0480023752.01.T01:exon:7816;Parent=MsG0480023752.01.T01
Chr4	exon	89399171	89399353	89399171	ID=MsG0480023752.01.T01:exon:7815;Parent=MsG0480023752.01.T01
Chr4	exon	89399444	89399909	89399444	ID=MsG0480023752.01.T01:exon:7814;Parent=MsG0480023752.01.T01
Chr4	five_prime_UTR	89399786	89399909	89399786	ID=MsG0480023752.01.T01:five_prime_utr;Parent=MsG0480023752.01.T01
Chr4	CDS	89399444	89399785	89399444	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89399171	89399353	89399171	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89398840	89398932	89398840	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89398580	89398732	89398580	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89397810	89397901	89397810	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89397144	89397285	89397144	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89396940	89397047	89396940	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89395379	89395470	89395379	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89395097	89395223	89395097	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89393982	89394068	89393982	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89392961	89393053	89392961	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89392520	89392675	89392520	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89391739	89391822	89391739	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89391005	89391085	89391005	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89390838	89390920	89390838	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89390651	89390768	89390651	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	CDS	89389416	89389679	89389416	ID=MsG0480023752.01.T01:cds;Parent=MsG0480023752.01.T01
Chr4	three_prime_UTR	89389266	89389415	89389266	ID=MsG0480023752.01.T01:three_prime_utr;Parent=MsG0480023752.01.T01
Chr4	three_prime_UTR	89388957	89388980	89388957	ID=MsG0480023752.01.T01:three_prime_utr;Parent=MsG0480023752.01.T01

Gene Sequence

>MsG0480023752.01.T01

ATGGCAATGGCTTGCATTCCTCACATTCTCTCTCCTCTTTCTCACTCTACAACACTTGTTAATCTCATCAACAAAACCAATTTCGAGATTCACAACAGAACCTCCATCACCATTTCCTCAAATTCAAGGTCAAAGAAGAAAACCTTCGCCGTTGGGAATCGTGTCGGTGTTGAGACTGAAACTGCAGTTATCGAAATTGAGAAGCGCCAATTGCAAGTTTCCAAAGGCTACCCTTCACCCTACGGTGCCACTCCTCAAGAAGACGGGGTCAATTTCGCCATTTACTCACTCAATTCACTCTCAGCTACTCTCTGCTTCTTCACTCTTTCTGACTTCAAAAACAATAAAGTGACGGAGTACATTACTCTTGATCCATTGCTGAATAAAACAGGATGTGTTTGGCATGTGTTTCTAAAAGGAGATTTTAAAGATATGCTCTATGCTTATAAATTTGATGGCAAATTTTCTCCTCAACACGGTCACTACTATGATTCTTCTCGTATTTTAATCGACCCTTACGCAAAAGCAGTTATAAGAAGAGGAGAGTTTGGGGAAGTAGGACCTGATGGTAATTGCTGGCCCCAAATGGCTGGTATGGTACCTTTTGATCATGAGGAGTTTGACTGGGAAGGTGACTTGCCTTTGAAATATCCACAAAAGGATCTTATTATATATGAAATGCATGTGAGAGGGTTTACAAAGCATGAGTCGAGTAAGGCCGAGTTTCCTGGTACATATCTTGGTGTCGTGGAGAAGCTTGATCACTTAAAGGAACTTGGAGTAAATTGTATAGAATTAATGCCATGTCATGAGTTCAATGAGCTGGAATACTACAGTTACAATGCTATTCAGGGGGACTATAGGGTCAATTTTTGGGGCTATTCAACCATCAATTACTTTTCACCAATGATAAGATACTCATCAGCTGGCATACAAAACTGTGGCCGTGATGGGATTAATGAAATGAAGCTCCTCATCAAAGAGGCACACAAGCGAGGAATAGAGGTTATCATGGATGTTGTTTTCAATCATACAGCTGAAGGGAATGAGAAGGGTCCCATTATTTCTTTTAGAGGAGTTGACAATAGCGTGTATTACATGGTGGCACCCAAGATTTGTTTCAATTATTATTTACAAGGGGCTAGAAATTGCTACCATATTAGATTGAAGGCCTTACTAATTTTCTCTCGTGCAACAGATATTGGTCTCTGGAACAGAGTTAATGTATTTGGTGCTCCAATAGAAGGTGACTTGCTGACAATTGGAACCCCTTTAAGCAGTCCGCCATTGATTGATATGATCAGTATTGATCCAATACTTCGTGGAGTGAAGCTTATAGCTGAAGCCTGGGATGCTGGAGGCCTGTACCAAGTTGGCACTTTCCCTCACTGGGGTATTTGGTCGGAATGGAATGGGAAGTACAGAGACACTGTACGCCAGTTTATCAAGGGTACAGATGGCTTTGCTGGAGCATTTGCTGAATGTATTTGTGGGAGTCCTAATTTGTATCAGGGAGGAAGAAAACCATGGAATAGTATTAACCTTGTATGTGCTCATGATGGCTTCACTCTAGCTGATTTGGTTACTTACAACAATAAGCATAACTTGCCCAATGGAGAAGACAACAATGATGGAGAAAATCACAATAGTAGCTGGAACTGTGGAGAGGAGGGGGAGTTTGTCAGTGCCTCAGTAAAGAAATTGAGGAAACGACAAATGCGTAATTTCTTTCTTTCTCTCATGGTTTCCCAGGGAGTTCCGATGATATATATGGGAGATGAATATGGACATACAAAAGGAGGAAACAACAATACCTACTGTCATGATAATTACCTTAATTATTTCCGATGGGACAAAAAGGAAGAATCCTCGTCAGACTTCTTCAGATTTTGCCGCCTTTTGACCAAGTTCCGCCAGGAATGTGAGTCGCTTGGCCTAGATGACTTCCCGACATCAGAGAGGCTTCAGTGGCATGGCCATTTTCCTGTAACACCAGACTGGTCTGAATCTAGCCGTTTTGTGGCTTTTACCCTGATGGACTCGGTGAAGGGAGAAATTTATGTTGCTTTCAATACAAGTCATTTACCTTTCACTGTTACCTTGCCAGAGCGTCCCGGATACAGATGGGAACCTCTCGTAGACACCAGCAAGAAAGCACCATATGATTTTCTCACACCTGATCTTCGCGGAAGAGATATTGCAATACAGCAGTATGCTCAGTTTCTTGACGCCAATATGTATCCCATGCTTAGTTACTCTTCAATCATCCTTTTGCGCACCCCCGATGTAAATGCTTAG

Protein sequence

>MsG0480023752.01.T01

MAMACIPHILSPLSHSTTLVNLINKTNFEIHNRTSITISSNSRSKKKTFAVGNRVGVETETAVIEIEKRQLQVSKGYPSPYGATPQEDGVNFAIYSLNSLSATLCFFTLSDFKNNKVTEYITLDPLLNKTGCVWHVFLKGDFKDMLYAYKFDGKFSPQHGHYYDSSRILIDPYAKAVIRRGEFGEVGPDGNCWPQMAGMVPFDHEEFDWEGDLPLKYPQKDLIIYEMHVRGFTKHESSKAEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNAIQGDYRVNFWGYSTINYFSPMIRYSSAGIQNCGRDGINEMKLLIKEAHKRGIEVIMDVVFNHTAEGNEKGPIISFRGVDNSVYYMVAPKICFNYYLQGARNCYHIRLKALLIFSRATDIGLWNRVNVFGAPIEGDLLTIGTPLSSPPLIDMISIDPILRGVKLIAEAWDAGGLYQVGTFPHWGIWSEWNGKYRDTVRQFIKGTDGFAGAFAECICGSPNLYQGGRKPWNSINLVCAHDGFTLADLVTYNNKHNLPNGEDNNDGENHNSSWNCGEEGEFVSASVKKLRKRQMRNFFLSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYLNYFRWDKKEESSSDFFRFCRLLTKFRQECESLGLDDFPTSERLQWHGHFPVTPDWSESSRFVAFTLMDSVKGEIYVAFNTSHLPFTVTLPERPGYRWEPLVDTSKKAPYDFLTPDLRGRDIAIQQYAQFLDANMYPMLSYSSIILLRTPDVNA*