AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005283.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005283.01.T01 MTR_1g096340 89.806 206 17 1 1 206 11 212 3.56e-134 375
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005283.01.T01 AT4G09620 57.073 205 82 3 5 206 9 210 4.40e-77 230
MsG0180005283.01.T01 AT4G09620 57.073 205 82 3 5 206 11 212 4.54e-77 230
MsG0180005283.01.T01 AT4G09620 57.073 205 82 3 5 206 11 212 4.54e-77 230

Find 57 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTGTTCATTCTCCTTAC+TGG 0.079985 1:+89651280 MsG0180005283.01.T01:CDS
GTCAAACAGGATTTATTATT+TGG 0.092352 1:+89652011 MsG0180005283.01.T01:three_prime_UTR
TCCATTTACTGCCTCAAATT+TGG 0.102598 1:-89651328 None:intergenic
ATTGCATGGTTCTCACATTT+TGG 0.171163 1:+89651702 MsG0180005283.01.T01:three_prime_UTR
CCTTCGAAATCCAAAAGTTT+TGG 0.174584 1:+89651518 MsG0180005283.01.T01:CDS
CCCAAATTTGAGGCAGTAAA+TGG 0.185063 1:+89651327 MsG0180005283.01.T01:CDS
AAATTACATCCAAGAACTTC+TGG 0.252185 1:-89651417 None:intergenic
ATATTAAAACCCAAAACTTT+TGG 0.261186 1:-89651528 None:intergenic
CCATTTACTGCCTCAAATTT+GGG 0.285378 1:-89651327 None:intergenic
CCTTGTTCATTCTCCTTACT+GGG 0.302250 1:+89651281 MsG0180005283.01.T01:CDS
GATATTTGGCGATGGAATAA+TGG 0.339231 1:-89651058 None:intergenic
CAATTTCCAACTCTGGCCTT+TGG 0.342176 1:-89650920 None:intergenic
CAATACTCCACTGCTCCTTC+AGG 0.365711 1:-89651470 None:intergenic
AAGATACTTGGAAAAGCATT+TGG 0.395197 1:+89651258 MsG0180005283.01.T01:CDS
ATGGAACATTAGATTATCTA+AGG 0.403508 1:+89651346 MsG0180005283.01.T01:CDS
ATCGTGCAGGCTGATCTTGC+TGG 0.416752 1:+89651027 MsG0180005283.01.T01:intron
ATTGTAATTTGTATCAACTT+AGG 0.421605 1:-89651944 None:intergenic
ACCTATTGATGATGTCAAAC+AGG 0.424650 1:+89651998 MsG0180005283.01.T01:three_prime_UTR
CGACTTCGCAAGTATGCATA+TGG 0.429873 1:+89651767 MsG0180005283.01.T01:three_prime_UTR
TGATTGTAAGGGAGATTGTA+AGG 0.441638 1:+89651551 MsG0180005283.01.T01:CDS
GTATTGAAGGAAAATCTCTT+AGG 0.453881 1:+89651487 MsG0180005283.01.T01:CDS
TCCTGTTTGACATCATCAAT+AGG 0.454728 1:-89651999 None:intergenic
AGGCACAATGCACTAGATGC+TGG 0.458361 1:+89651571 MsG0180005283.01.T01:CDS
TAACTTCGACGATGAAGAAA+AGG 0.469350 1:+89651233 MsG0180005283.01.T01:CDS
TATGAATTGATGAAGTTACT+TGG 0.469958 1:+89651804 MsG0180005283.01.T01:three_prime_UTR
AATGTTACAATTTCCAACTC+TGG 0.472025 1:-89650927 None:intergenic
AAGGAGCAGTGGAGTATTGA+AGG 0.472993 1:+89651474 MsG0180005283.01.T01:CDS
TGGGTTCGATTCTAGTTGAT+TGG 0.477953 1:+89651591 MsG0180005283.01.T01:exon
GAAGAAAAGGACAAGATACT+TGG 0.482017 1:+89651246 MsG0180005283.01.T01:CDS
AATGAATTATTTCTGCAACT+TGG 0.490911 1:-89651728 None:intergenic
TGGCGATGGAATAATGGAAA+AGG 0.502232 1:-89651052 None:intergenic
GTGATGAAGATCGGAGTATG+TGG 0.505034 1:+89651181 MsG0180005283.01.T01:CDS
TTGACATCATCAATAGGTTG+AGG 0.508507 1:-89651993 None:intergenic
TCACGATCTTCTCCCCAGTA+AGG 0.522126 1:-89651294 None:intergenic
TGACATCATCAATAGGTTGA+GGG 0.525521 1:-89651992 None:intergenic
CAAATCATTCTACCAACCAT+TGG 0.536249 1:+89651118 MsG0180005283.01.T01:CDS
TGATGAAGTTACTTGGACAA+TGG 0.538545 1:+89651811 MsG0180005283.01.T01:three_prime_UTR
ACAGATCCAAAGGCCAGAGT+TGG 0.550716 1:+89650914 MsG0180005283.01.T01:CDS
GAACAATGATGTTATGCTGC+AGG 0.558364 1:-89651643 None:intergenic
GGCACAATGCACTAGATGCT+GGG 0.562129 1:+89651572 MsG0180005283.01.T01:CDS
GAAGGAAGTTCCCAAATTTG+AGG 0.566069 1:+89651317 MsG0180005283.01.T01:CDS
GAACGAAGATACTCACAGTA+AGG 0.568225 1:+89651833 MsG0180005283.01.T01:three_prime_UTR
TAATCTCAAGTGATGAAGAT+CGG 0.572690 1:+89651172 MsG0180005283.01.T01:CDS
TTTATTTACGGTGGAGAGGG+CGG 0.581343 1:-89651672 None:intergenic
CCCAGTAAGGAGAATGAACA+AGG 0.586733 1:-89651281 None:intergenic
CTTGTTCATTCTCCTTACTG+GGG 0.596220 1:+89651282 MsG0180005283.01.T01:CDS
TAAAGATCCTGAAGGAGCAG+TGG 0.596954 1:+89651463 MsG0180005283.01.T01:CDS
ACTTATGCATACAGATCCAA+AGG 0.602167 1:+89650904 None:intergenic
ATGGTTGGTAGAATGATTTG+AGG 0.603312 1:-89651115 None:intergenic
AGAACGCAATATCCAATGGT+TGG 0.612381 1:-89651130 None:intergenic
TCGTAGAACGCAATATCCAA+TGG 0.625607 1:-89651134 None:intergenic
AGCTAATATAAAGATCCTGA+AGG 0.630758 1:+89651455 MsG0180005283.01.T01:CDS
CTGGGGAGAAGATCGTGAGA+AGG 0.634124 1:+89651299 MsG0180005283.01.T01:CDS
CAAGTCTTGCAAAAGCAAAG+TGG 0.634464 1:+89651091 MsG0180005283.01.T01:CDS
TGATGAAGATCGGAGTATGT+GGG 0.637812 1:+89651182 MsG0180005283.01.T01:CDS
AAATAAAAGACAGAATTGCA+TGG 0.657141 1:+89651688 MsG0180005283.01.T01:three_prime_UTR
AATTCATTCTCAAGTTAACG+CGG 0.707885 1:+89651743 MsG0180005283.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTAAAACCCAAAACTTT+TGG - Chr1:89651531-89651550 None:intergenic 20.0%
!!! ATTGTAATTTGTATCAACTT+AGG - Chr1:89651947-89651966 None:intergenic 20.0%
!!! GGTTTTAATATTGATTGTAA+GGG + Chr1:89651540-89651559 MsG0180005283.01.T01:CDS 20.0%
! AAAAAATTTCCAGAAGTTCT+TGG + Chr1:89651408-89651427 MsG0180005283.01.T01:CDS 25.0%
! AAATAAAAGACAGAATTGCA+TGG + Chr1:89651688-89651707 MsG0180005283.01.T01:three_prime_UTR 25.0%
! ATGGAACATTAGATTATCTA+AGG + Chr1:89651346-89651365 MsG0180005283.01.T01:CDS 25.0%
! ATGTAAAAAAATTCACTGGT+AGG + Chr1:89650957-89650976 MsG0180005283.01.T01:intron 25.0%
! CAAGATGTAAAAAAATTCAC+TGG + Chr1:89650953-89650972 MsG0180005283.01.T01:intron 25.0%
! GTCAAACAGGATTTATTATT+TGG + Chr1:89652011-89652030 MsG0180005283.01.T01:three_prime_UTR 25.0%
! TATGAATTGATGAAGTTACT+TGG + Chr1:89651804-89651823 MsG0180005283.01.T01:three_prime_UTR 25.0%
!!! AATGAATTATTTCTGCAACT+TGG - Chr1:89651731-89651750 None:intergenic 25.0%
!!! GCAATTCTGTCTTTTATTTA+CGG - Chr1:89651687-89651706 None:intergenic 25.0%
!!! GGGTTTTAATATTGATTGTA+AGG + Chr1:89651539-89651558 MsG0180005283.01.T01:CDS 25.0%
!!! TGGAAATTTTTTAAGCAACT+TGG - Chr1:89651400-89651419 None:intergenic 25.0%
AAAAATCTGATATTTGGCGA+TGG - Chr1:89651069-89651088 None:intergenic 30.0%
AAATTACATCCAAGAACTTC+TGG - Chr1:89651420-89651439 None:intergenic 30.0%
AATGTTACAATTTCCAACTC+TGG - Chr1:89650930-89650949 None:intergenic 30.0%
AATTCATTCTCAAGTTAACG+CGG + Chr1:89651743-89651762 MsG0180005283.01.T01:three_prime_UTR 30.0%
AGCTAATATAAAGATCCTGA+AGG + Chr1:89651455-89651474 MsG0180005283.01.T01:CDS 30.0%
CTTGGCAAAAATCTGATATT+TGG - Chr1:89651075-89651094 None:intergenic 30.0%
GTATTGAAGGAAAATCTCTT+AGG + Chr1:89651487-89651506 MsG0180005283.01.T01:CDS 30.0%
TAATCTCAAGTGATGAAGAT+CGG + Chr1:89651172-89651191 MsG0180005283.01.T01:CDS 30.0%
! CTTCGAAATCCAAAAGTTTT+GGG + Chr1:89651519-89651538 MsG0180005283.01.T01:CDS 30.0%
!! AAGATACTTGGAAAAGCATT+TGG + Chr1:89651258-89651277 MsG0180005283.01.T01:CDS 30.0%
!! ATTCTGTCTTTTATTTACGG+TGG - Chr1:89651684-89651703 None:intergenic 30.0%
CAAATCATTCTACCAACCAT+TGG + Chr1:89651118-89651137 MsG0180005283.01.T01:CDS 35.0%
CCATTTACTGCCTCAAATTT+GGG - Chr1:89651330-89651349 None:intergenic 35.0%
CGTACACAATATTTAGACTC+TGG - Chr1:89651007-89651026 None:intergenic 35.0%
GAAGAAAAGGACAAGATACT+TGG + Chr1:89651246-89651265 MsG0180005283.01.T01:CDS 35.0%
GATATTTGGCGATGGAATAA+TGG - Chr1:89651061-89651080 None:intergenic 35.0%
TAACTTCGACGATGAAGAAA+AGG + Chr1:89651233-89651252 MsG0180005283.01.T01:CDS 35.0%
TCCATTTACTGCCTCAAATT+TGG - Chr1:89651331-89651350 None:intergenic 35.0%
TCCTGTTTGACATCATCAAT+AGG - Chr1:89652002-89652021 None:intergenic 35.0%
TGATGAAGTTACTTGGACAA+TGG + Chr1:89651811-89651830 MsG0180005283.01.T01:three_prime_UTR 35.0%
TGATTGTAAGGGAGATTGTA+AGG + Chr1:89651551-89651570 MsG0180005283.01.T01:CDS 35.0%
TTGACATCATCAATAGGTTG+AGG - Chr1:89651996-89652015 None:intergenic 35.0%
! ATTGCATGGTTCTCACATTT+TGG + Chr1:89651702-89651721 MsG0180005283.01.T01:three_prime_UTR 35.0%
! CCAAAACTTTTGGATTTCGA+AGG - Chr1:89651521-89651540 None:intergenic 35.0%
! CCTTCGAAATCCAAAAGTTT+TGG + Chr1:89651518-89651537 MsG0180005283.01.T01:CDS 35.0%
! TGACATCATCAATAGGTTGA+GGG - Chr1:89651995-89652014 None:intergenic 35.0%
!! ACCTATTGATGATGTCAAAC+AGG + Chr1:89651998-89652017 MsG0180005283.01.T01:three_prime_UTR 35.0%
!! ATGGTTGGTAGAATGATTTG+AGG - Chr1:89651118-89651137 None:intergenic 35.0%
!! TCTTTTATTTACGGTGGAGA+GGG - Chr1:89651678-89651697 None:intergenic 35.0%
AAATTCACTGGTAGGACTGT+AGG + Chr1:89650965-89650984 MsG0180005283.01.T01:intron 40.0%
AATATTGTGTACGATCGTGC+AGG + Chr1:89651014-89651033 MsG0180005283.01.T01:intron 40.0%
AGAACGCAATATCCAATGGT+TGG - Chr1:89651133-89651152 None:intergenic 40.0%
CAAGTCTTGCAAAAGCAAAG+TGG + Chr1:89651091-89651110 MsG0180005283.01.T01:CDS 40.0%
CCTTGTTCATTCTCCTTACT+GGG + Chr1:89651281-89651300 MsG0180005283.01.T01:CDS 40.0%
CTTGTTCATTCTCCTTACTG+GGG + Chr1:89651282-89651301 MsG0180005283.01.T01:CDS 40.0%
GAACAATGATGTTATGCTGC+AGG - Chr1:89651646-89651665 None:intergenic 40.0%
GAACGAAGATACTCACAGTA+AGG + Chr1:89651833-89651852 MsG0180005283.01.T01:three_prime_UTR 40.0%
GAAGGAAGTTCCCAAATTTG+AGG + Chr1:89651317-89651336 MsG0180005283.01.T01:CDS 40.0%
GGACTGTAGGAGGATAATAA+TGG + Chr1:89650978-89650997 MsG0180005283.01.T01:intron 40.0%
TCGTAGAACGCAATATCCAA+TGG - Chr1:89651137-89651156 None:intergenic 40.0%
TGATGAAGATCGGAGTATGT+GGG + Chr1:89651182-89651201 MsG0180005283.01.T01:CDS 40.0%
! CCCAAATTTGAGGCAGTAAA+TGG + Chr1:89651327-89651346 MsG0180005283.01.T01:CDS 40.0%
! CTTTTGGATTTCGAAGGAGA+AGG - Chr1:89651515-89651534 None:intergenic 40.0%
! TGGCGATGGAATAATGGAAA+AGG - Chr1:89651055-89651074 None:intergenic 40.0%
! TGGGTTCGATTCTAGTTGAT+TGG + Chr1:89651591-89651610 MsG0180005283.01.T01:exon 40.0%
!! CACTTTGCTTTTGCAAGACT+TGG - Chr1:89651093-89651112 None:intergenic 40.0%
!! GTCTTTTATTTACGGTGGAG+AGG - Chr1:89651679-89651698 None:intergenic 40.0%
CCCAGTAAGGAGAATGAACA+AGG - Chr1:89651284-89651303 None:intergenic 45.0%
CGACTTCGCAAGTATGCATA+TGG + Chr1:89651767-89651786 MsG0180005283.01.T01:three_prime_UTR 45.0%
GTGATGAAGATCGGAGTATG+TGG + Chr1:89651181-89651200 MsG0180005283.01.T01:CDS 45.0%
TAAAGATCCTGAAGGAGCAG+TGG + Chr1:89651463-89651482 MsG0180005283.01.T01:CDS 45.0%
TTTATTTACGGTGGAGAGGG+CGG - Chr1:89651675-89651694 None:intergenic 45.0%
! CAATTTCCAACTCTGGCCTT+TGG - Chr1:89650923-89650942 None:intergenic 45.0%
! GCCTTGTTCATTCTCCTTAC+TGG + Chr1:89651280-89651299 MsG0180005283.01.T01:CDS 45.0%
!! AAGGAGCAGTGGAGTATTGA+AGG + Chr1:89651474-89651493 MsG0180005283.01.T01:CDS 45.0%
ACAGATCCAAAGGCCAGAGT+TGG + Chr1:89650914-89650933 MsG0180005283.01.T01:CDS 50.0%
AGGCACAATGCACTAGATGC+TGG + Chr1:89651571-89651590 MsG0180005283.01.T01:CDS 50.0%
CAATACTCCACTGCTCCTTC+AGG - Chr1:89651473-89651492 None:intergenic 50.0%
GGCACAATGCACTAGATGCT+GGG + Chr1:89651572-89651591 MsG0180005283.01.T01:CDS 50.0%
TCACGATCTTCTCCCCAGTA+AGG - Chr1:89651297-89651316 None:intergenic 50.0%
TTCACTGGTAGGACTGTAGG+AGG + Chr1:89650968-89650987 MsG0180005283.01.T01:intron 50.0%
ATCGTGCAGGCTGATCTTGC+TGG + Chr1:89651027-89651046 MsG0180005283.01.T01:intron 55.0%
CTGGGGAGAAGATCGTGAGA+AGG + Chr1:89651299-89651318 MsG0180005283.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89650908 89652036 89650908 ID=MsG0180005283.01;Name=MsG0180005283.01
Chr1 mRNA 89650908 89652036 89650908 ID=MsG0180005283.01.T01;Parent=MsG0180005283.01;Name=MsG0180005283.01.T01;_AED=0.36;_eAED=0.40;_QI=0|0|0.5|1|1|1|2|431|206
Chr1 exon 89650908 89650958 89650908 ID=MsG0180005283.01.T01:exon:17240;Parent=MsG0180005283.01.T01
Chr1 exon 89651036 89652036 89651036 ID=MsG0180005283.01.T01:exon:17241;Parent=MsG0180005283.01.T01
Chr1 CDS 89650908 89650958 89650908 ID=MsG0180005283.01.T01:cds;Parent=MsG0180005283.01.T01
Chr1 CDS 89651036 89651605 89651036 ID=MsG0180005283.01.T01:cds;Parent=MsG0180005283.01.T01
Chr1 three_prime_UTR 89651606 89652036 89651606 ID=MsG0180005283.01.T01:three_prime_utr;Parent=MsG0180005283.01.T01
Gene Sequence

>MsG0180005283.01.T01

ATGCATACAGATCCAAAGGCCAGAGTTGGAAATTGTAACATTCAACAAGATGCTGATCTTGCTGGTGCCTTTTCCATTATTCCATCGCCAAATATCAGATTTTTGCCAAGTCTTGCAAAAGCAAAGTGGACCTCAAATCATTCTACCAACCATTGGATATTGCGTTCTACGACACAGATAGAGAATGTAATCTCAAGTGATGAAGATCGGAGTATGTGGGAAGCATGTAGACAAGCTCTTTCTGCATTTAACTTCGACGATGAAGAAAAGGACAAGATACTTGGAAAAGCATTTGGCCTTGTTCATTCTCCTTACTGGGGAGAAGATCGTGAGAAGGAAGTTCCCAAATTTGAGGCAGTAAATGGAACATTAGATTATCTAAGGAGTTTAAATCTTTCTGACGATGATTTGTCCAAGTTGCTTAAAAAATTTCCAGAAGTTCTTGGATGTAATTTAGAAGAAGAGTTGAAAGCTAATATAAAGATCCTGAAGGAGCAGTGGAGTATTGAAGGAAAATCTCTTAGGAACCTTCTCCTTCGAAATCCAAAAGTTTTGGGTTTTAATATTGATTGTAAGGGAGATTGTAAGGCACAATGCACTAGATGCTGGGTTCGATTCTAG

Protein sequence

>MsG0180005283.01.T01

MHTDPKARVGNCNIQQDADLAGAFSIIPSPNIRFLPSLAKAKWTSNHSTNHWILRSTTQIENVISSDEDRSMWEACRQALSAFNFDDEEKDKILGKAFGLVHSPYWGEDREKEVPKFEAVNGTLDYLRSLNLSDDDLSKLLKKFPEVLGCNLEEELKANIKILKEQWSIEGKSLRNLLLRNPKVLGFNIDCKGDCKAQCTRCWVRF*