AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007372.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007372.01.T01 MTR_2g023880 92.806 139 10 0 182 320 84 222 1.59e-84 254
MsG0280007372.01.T01 MTR_2g023880 67.516 157 38 2 1 144 1 157 4.03e-62 197
MsG0280007372.01.T01 MTR_4g049540 57.143 126 54 0 194 319 95 220 5.31e-44 150
MsG0280007372.01.T01 MTR_4g049540 59.649 114 44 2 1 113 1 113 3.37e-39 138
MsG0280007372.01.T01 MTR_2g088060 47.619 126 65 1 194 319 96 220 2.54e-30 114
MsG0280007372.01.T01 MTR_2g088060 51.754 114 54 1 1 113 1 114 3.03e-25 101
MsG0280007372.01.T01 MTR_5g093360 46.957 115 55 2 1 115 1 109 1.21e-29 109
MsG0280007372.01.T01 MTR_7g056133 44.531 128 65 4 1 123 1 127 6.17e-23 95.1
MsG0280007372.01.T01 MTR_7g056133 37.607 117 66 1 194 303 96 212 2.45e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007372.01.T01 AT5G42570 60.526 114 45 0 194 307 95 208 2.66e-42 146
MsG0280007372.01.T01 AT5G42570 62.281 114 41 2 1 113 1 113 4.74e-41 142
MsG0280007372.01.T01 AT1G11905 62.500 104 38 1 1 104 1 103 4.03e-37 132
MsG0280007372.01.T01 AT1G11905 55.652 115 51 0 194 308 95 209 1.13e-35 129
MsG0280007372.01.T01 AT1G11905 64.211 95 33 1 1 95 1 94 1.50e-34 125
MsG0280007372.01.T01 AT1G11905 52.941 102 48 0 207 308 107 208 5.29e-28 108
MsG0280007372.01.T01 AT5G48660 45.312 128 64 4 1 123 1 127 6.09e-24 97.8
MsG0280007372.01.T01 AT3G07190 43.651 126 65 3 1 121 1 125 5.17e-22 92.8
MsG0280007372.01.T01 AT3G20450 43.860 114 63 1 1 113 5 118 1.58e-21 89.0

Find 68 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCTCACATATTCTTCT+TGG 0.219887 2:+14673916 None:intergenic
TCCTACCGATCAGGTTCTTA+TGG 0.252341 2:-14676283 MsG0280007372.01.T01:CDS
GTAGCTGGTTGCGGAGGTTT+TGG 0.307160 2:+14673972 None:intergenic
ATTGAGGAAGCTTGTAATAA+TGG 0.322813 2:-14676445 MsG0280007372.01.T01:CDS
CTATCATAAGTGCAAGAAAA+AGG 0.327998 2:+14674275 None:intergenic
ATTCTTGGTCAATTATTTGC+AGG 0.338690 2:-14674304 MsG0280007372.01.T01:CDS
GGGGCTGAGGATGCGAAAGC+TGG 0.347270 2:-14674178 MsG0280007372.01.T01:CDS
TTGCGAGCATTATTCATTCT+TGG 0.348203 2:-14674319 MsG0280007372.01.T01:CDS
GAAGCTTGTAATAATGGCTT+TGG 0.364769 2:-14676439 MsG0280007372.01.T01:CDS
CCATGCTCTTTCTCCGAATC+CGG 0.367578 2:+14674224 None:intergenic
AGGGCAAAGCCTCTCGGACT+GGG 0.378306 2:+14675546 None:intergenic
CAGGGCAAAGCCTCTCGGAC+TGG 0.391453 2:+14675545 None:intergenic
TTATTACAAGCTTCCTCAAT+GGG 0.391866 2:+14676448 None:intergenic
AAGCCAGTGTAAGTGCTCTA+AGG 0.421670 2:-14674045 MsG0280007372.01.T01:CDS
TTCTGTTAATCCTACCGATC+AGG 0.442382 2:-14676292 MsG0280007372.01.T01:CDS
ACAGACTGCATCATTACATA+AGG 0.453424 2:-14674252 MsG0280007372.01.T01:CDS
TCTTGAGTTTACTGTGCCTC+CGG 0.456209 2:-14675576 MsG0280007372.01.T01:CDS
ACATAAGGGAACTCCGGATT+CGG 0.458900 2:-14674237 MsG0280007372.01.T01:CDS
ACGAGGTCGTGGACCTGTTA+TGG 0.464283 2:-14676406 MsG0280007372.01.T01:CDS
TCTAGTTTCCGTATCGGATC+CGG 0.469533 2:+14675963 None:intergenic
TTCTGTCAACAGATTGTAGC+TGG 0.470135 2:+14673957 None:intergenic
ATTATTACAAGCTTCCTCAA+TGG 0.471169 2:+14676447 None:intergenic
GTCAAGCACCGGATCCGATA+CGG 0.483983 2:-14675971 MsG0280007372.01.T01:CDS
TCTCGGACTGGGGAAAGAGC+CGG 0.488678 2:+14675557 None:intergenic
GAGAGCAGAAGTTGATCGTC+TGG 0.490422 2:-14674110 MsG0280007372.01.T01:CDS
CAACAGATTGTAGCTGGTTG+CGG 0.507849 2:+14673963 None:intergenic
GGCTGCCTTCAATTCTCGAA+TGG 0.512945 2:-14675600 MsG0280007372.01.T01:CDS
GTTATGGTGAAAACCGTTGC+TGG 0.514910 2:-14676390 MsG0280007372.01.T01:CDS
AAGAACCATTCGAGAATTGA+AGG 0.522112 2:+14675595 None:intergenic
TTCAGATGAAACTAAAACAA+TGG 0.522908 2:-14674152 MsG0280007372.01.T01:CDS
GATCGGTTGAAACGAGGTCG+TGG 0.523142 2:-14676417 MsG0280007372.01.T01:CDS
ACTTCAGTCAAAAGATAAAG+AGG 0.526615 2:-14674080 MsG0280007372.01.T01:CDS
CAACAACGTGGGATCGAAGA+TGG 0.533524 2:-14676318 MsG0280007372.01.T01:CDS
TGAATATGATCGCTTACTTG+AGG 0.542275 2:-14673999 MsG0280007372.01.T01:CDS
TTGTAATAATGGCTTTGGAT+CGG 0.542465 2:-14676434 MsG0280007372.01.T01:CDS
TGCTTATAATTAAGACTGGA+CGG 0.542553 2:-14676001 MsG0280007372.01.T01:intron
AGCTGTTAAGAAACAAATGC+GGG 0.550293 2:-14674200 MsG0280007372.01.T01:CDS
TCTTCTTGAAGCTACACTCA+TGG 0.550488 2:-14676253 MsG0280007372.01.T01:CDS
TTTGACAGGGCAAAGCCTCT+CGG 0.550817 2:+14675540 None:intergenic
CAGACTGCATCATTACATAA+GGG 0.555282 2:-14674251 MsG0280007372.01.T01:CDS
CTTCTTGAAGCTACACTCAT+GGG 0.557141 2:-14676252 MsG0280007372.01.T01:CDS
GGGCAAAGCCTCTCGGACTG+GGG 0.564491 2:+14675547 None:intergenic
CAGATTGTAGCTGGTTGCGG+AGG 0.567147 2:+14673966 None:intergenic
GCTCTAAGGAAACAATCTGA+AGG 0.569864 2:-14674031 MsG0280007372.01.T01:CDS
GTCATATCTAGTTTCCGTAT+CGG 0.570023 2:+14675957 None:intergenic
ATCATTACATAAGGGAACTC+CGG 0.575747 2:-14674243 MsG0280007372.01.T01:CDS
GCCATAAGAACCTGATCGGT+AGG 0.586898 2:+14676282 None:intergenic
CTCTAAGGAAACAATCTGAA+GGG 0.587548 2:-14674030 MsG0280007372.01.T01:CDS
CAGTCAAAAGATAAAGAGGT+AGG 0.596716 2:-14674076 MsG0280007372.01.T01:CDS
CCGGATTCGGAGAAAGAGCA+TGG 0.597596 2:-14674224 MsG0280007372.01.T01:CDS
GCTTTGGATCGGTTGAAACG+AGG 0.607033 2:-14676423 MsG0280007372.01.T01:CDS
TTTCCTTAGAGCACTTACAC+TGG 0.609208 2:+14674042 None:intergenic
GGCTCTTTCCCCAGTCCGAG+AGG 0.610643 2:-14675555 MsG0280007372.01.T01:CDS
CTTAGCCATAAGAACCTGAT+CGG 0.613122 2:+14676278 None:intergenic
ACGGAGTATCTGTCAAGCAC+CGG 0.614047 2:-14675982 MsG0280007372.01.T01:CDS
AACAAGAACAGTACCAGCAA+CGG 0.621936 2:+14676377 None:intergenic
CGGACTGGGGAAAGAGCCGG+AGG 0.627493 2:+14675560 None:intergenic
CTGTTAAGAAACAAATGCGG+GGG 0.642686 2:-14674198 MsG0280007372.01.T01:CDS
TGTTATTCAAGACCCCATTG+AGG 0.652558 2:-14676461 MsG0280007372.01.T01:CDS
TGATGAAGATTCAACAACGT+GGG 0.655476 2:-14676329 MsG0280007372.01.T01:CDS
TGACACTGACACAGACACGT+TGG 0.666836 2:-14675938 MsG0280007372.01.T01:CDS
GCTGTTAAGAAACAAATGCG+GGG 0.673517 2:-14674199 MsG0280007372.01.T01:CDS
TTATTAAAAGATATCCTCTG+TGG 0.683349 2:-14674343 MsG0280007372.01.T01:CDS
TATTACAAGCTTCCTCAATG+GGG 0.687342 2:+14676449 None:intergenic
AAGCTGTTAAGAAACAAATG+CGG 0.709469 2:-14674201 MsG0280007372.01.T01:CDS
TGTTAAGAAACAAATGCGGG+GGG 0.715839 2:-14674197 MsG0280007372.01.T01:CDS
ATGATGAAGATTCAACAACG+TGG 0.740230 2:-14676330 MsG0280007372.01.T01:CDS
ATAATGCTCGCAAGCCACAG+AGG 0.744250 2:+14674329 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCATAATATGAAATAAAA+GGG + Chr2:14675124-14675143 None:intergenic 10.0%
!! TATTCATAATATGAAATAAA+AGG + Chr2:14675125-14675144 None:intergenic 10.0%
!! TTTAATAAGTCTAAATATTA+AGG + Chr2:14676083-14676102 None:intergenic 10.0%
!! TATAGTATTAAACTTATTAG+TGG - Chr2:14675205-14675224 MsG0280007372.01.T01:intron 15.0%
!!! TTGAATTTGATTTATGTTTA+TGG - Chr2:14674290-14674309 MsG0280007372.01.T01:CDS 15.0%
!!! TTTTACTAGTTGTATATAAT+TGG - Chr2:14675073-14675092 MsG0280007372.01.T01:intron 15.0%
!!! TTTTGTAAATATGATCAAAT+AGG - Chr2:14674389-14674408 MsG0280007372.01.T01:intron 15.0%
!! AATCATAAGATATATGCATA+TGG + Chr2:14675443-14675462 None:intergenic 20.0%
!! ATTGTTTATTGATGAGTTAT+TGG - Chr2:14675777-14675796 MsG0280007372.01.T01:intron 20.0%
!! TTCTAAACCATTGAAAAAAT+CGG + Chr2:14675671-14675690 None:intergenic 20.0%
!! TTGATTTATGTTTATGGATT+TGG - Chr2:14674296-14674315 MsG0280007372.01.T01:CDS 20.0%
!!! AGTATTTCTTTTGAATAGTA+TGG - Chr2:14675180-14675199 MsG0280007372.01.T01:intron 20.0%
!!! ATGGGTATGTTTTTTTTAAA+TGG - Chr2:14674204-14674223 MsG0280007372.01.T01:CDS 20.0%
!!! ATTTTGCTTATAATTAAGAC+TGG - Chr2:14674433-14674452 MsG0280007372.01.T01:intron 20.0%
!!! CTATATGATTTGCAATTTTT+AGG - Chr2:14675832-14675851 MsG0280007372.01.T01:intron 20.0%
!!! GTTATTGTTTTACTATTGAT+AGG + Chr2:14675053-14675072 None:intergenic 20.0%
!!! TCTTTGATTTTTAATAAGAC+CGG - Chr2:14675969-14675988 MsG0280007372.01.T01:CDS 20.0%
!!! TGGGTATGTTTTTTTTAAAT+GGG - Chr2:14674205-14674224 MsG0280007372.01.T01:CDS 20.0%
! AACATATTCATCACAATGTA+AGG + Chr2:14674732-14674751 None:intergenic 25.0%
! AAGTTTAACAACATACTAGA+TGG + Chr2:14675881-14675900 None:intergenic 25.0%
! ACTACATTAATGAAAGGAAA+AGG + Chr2:14674706-14674725 None:intergenic 25.0%
! CTACATTAATGAAAGGAAAA+GGG + Chr2:14674705-14674724 None:intergenic 25.0%
! TTATTAAAAGATATCCTCTG+TGG - Chr2:14676095-14676114 MsG0280007372.01.T01:intron 25.0%
! TTCAGATGAAACTAAAACAA+TGG - Chr2:14676286-14676305 MsG0280007372.01.T01:CDS 25.0%
! TTTGGAAATATGTTGAACTT+AGG + Chr2:14675928-14675947 None:intergenic 25.0%
!! AATCATTGTGATTCTTTTTC+AGG + Chr2:14675389-14675408 None:intergenic 25.0%
!! GAATGAATGAATTTTGTATG+AGG - Chr2:14674265-14674284 MsG0280007372.01.T01:CDS 25.0%
!!! CAGCAATGTTTTGAAAAATT+TGG + Chr2:14675946-14675965 None:intergenic 25.0%
!!! GAAGTTTTGAGTAATGTTTA+AGG - Chr2:14675151-14675170 MsG0280007372.01.T01:intron 25.0%
AAGCTGTTAAGAAACAAATG+CGG - Chr2:14676237-14676256 MsG0280007372.01.T01:CDS 30.0%
AAGTTGAAAAATGTGAGCTT+TGG - Chr2:14675731-14675750 MsG0280007372.01.T01:intron 30.0%
ACAATGATTCAGCATTCATA+AGG - Chr2:14675400-14675419 MsG0280007372.01.T01:intron 30.0%
ACTTCAGTCAAAAGATAAAG+AGG - Chr2:14676358-14676377 MsG0280007372.01.T01:CDS 30.0%
AGATCTTCTCAATTTCCAAA+AGG + Chr2:14674638-14674657 None:intergenic 30.0%
AGGTTGACTACATTAATGAA+AGG + Chr2:14674712-14674731 None:intergenic 30.0%
ATTATTACAAGCTTCCTCAA+TGG + Chr2:14673994-14674013 None:intergenic 30.0%
ATTCTTGGTCAATTATTTGC+AGG - Chr2:14676134-14676153 MsG0280007372.01.T01:intron 30.0%
ATTGAGGAAGCTTGTAATAA+TGG - Chr2:14673993-14674012 MsG0280007372.01.T01:CDS 30.0%
CAGTATTGTTTATGTTGGAT+TGG + Chr2:14676038-14676057 None:intergenic 30.0%
CTATCATAAGTGCAAGAAAA+AGG + Chr2:14676166-14676185 None:intergenic 30.0%
CTCAACAGTATTGTTTATGT+TGG + Chr2:14676043-14676062 None:intergenic 30.0%
GAATAACCCAACAAAGAATT+AGG + Chr2:14675621-14675640 None:intergenic 30.0%
GTTTATTGATGAGTTATTGG+TGG - Chr2:14675780-14675799 MsG0280007372.01.T01:intron 30.0%
TGCTTATAATTAAGACTGGA+CGG - Chr2:14674437-14674456 MsG0280007372.01.T01:intron 30.0%
TGTTTGCCTAATTCTTTGTT+GGG - Chr2:14675612-14675631 MsG0280007372.01.T01:intron 30.0%
TTAAAACAACATAGCTGAAG+AGG - Chr2:14675999-14676018 MsG0280007372.01.T01:intron 30.0%
TTATTACAAGCTTCCTCAAT+GGG + Chr2:14673993-14674012 None:intergenic 30.0%
TTGTTTGCCTAATTCTTTGT+TGG - Chr2:14675611-14675630 MsG0280007372.01.T01:intron 30.0%
! ATGGTTTAGAATGAATGTTG+AGG - Chr2:14675680-14675699 MsG0280007372.01.T01:intron 30.0%
! TGATCAATCACAAAACCTTT+TGG - Chr2:14674620-14674639 MsG0280007372.01.T01:intron 30.0%
!! ACATTTGTAAGAGTGTTGTA+CGG - Chr2:14674554-14674573 MsG0280007372.01.T01:intron 30.0%
!! TTGTAATAATGGCTTTGGAT+CGG - Chr2:14674004-14674023 MsG0280007372.01.T01:CDS 30.0%
!!! ATGTTGTTTTAAGATGTGAC+CGG + Chr2:14675991-14676010 None:intergenic 30.0%
!!! GATTCTTTTTCAGGAAAACA+CGG + Chr2:14675380-14675399 None:intergenic 30.0%
!!! TTTGCTACCGATTTTTTCAA+TGG - Chr2:14675661-14675680 MsG0280007372.01.T01:intron 30.0%
AAGAACCATTCGAGAATTGA+AGG + Chr2:14674846-14674865 None:intergenic 35.0%
ACAGACTGCATCATTACATA+AGG - Chr2:14676186-14676205 MsG0280007372.01.T01:intron 35.0%
ACTCCGAAAGATCAGAAAAT+GGG + Chr2:14674676-14674695 None:intergenic 35.0%
AGCTGTTAAGAAACAAATGC+GGG - Chr2:14676238-14676257 MsG0280007372.01.T01:CDS 35.0%
ATCATTACATAAGGGAACTC+CGG - Chr2:14676195-14676214 MsG0280007372.01.T01:intron 35.0%
ATGATGAAGATTCAACAACG+TGG - Chr2:14674108-14674127 MsG0280007372.01.T01:CDS 35.0%
CAGACTGCATCATTACATAA+GGG - Chr2:14676187-14676206 MsG0280007372.01.T01:intron 35.0%
CAGTCAAAAGATAAAGAGGT+AGG - Chr2:14676362-14676381 MsG0280007372.01.T01:CDS 35.0%
CTGTTGTCAATGCAGAAATA+CGG + Chr2:14675266-14675285 None:intergenic 35.0%
GAAGCTTGTAATAATGGCTT+TGG - Chr2:14673999-14674018 MsG0280007372.01.T01:CDS 35.0%
GAGAATGTTCTACTTTCTTG+TGG - Chr2:14675753-14675772 MsG0280007372.01.T01:intron 35.0%
TATTACAAGCTTCCTCAATG+GGG + Chr2:14673992-14674011 None:intergenic 35.0%
TGAATATGATCGCTTACTTG+AGG - Chr2:14676439-14676458 MsG0280007372.01.T01:CDS 35.0%
TGATGAAGATTCAACAACGT+GGG - Chr2:14674109-14674128 MsG0280007372.01.T01:CDS 35.0%
TTCAGCATTCATAAGGCATA+AGG - Chr2:14675407-14675426 MsG0280007372.01.T01:intron 35.0%
TTGCGAGCATTATTCATTCT+TGG - Chr2:14676119-14676138 MsG0280007372.01.T01:intron 35.0%
! CTCTAAGGAAACAATCTGAA+GGG - Chr2:14676408-14676427 MsG0280007372.01.T01:CDS 35.0%
! GAACCCATTTTCTGATCTTT+CGG - Chr2:14674670-14674689 MsG0280007372.01.T01:intron 35.0%
! GTCATATCTAGTTTCCGTAT+CGG + Chr2:14674484-14674503 None:intergenic 35.0%
!!! TGGTATTTTAGCCTCCTAAT+GGG - Chr2:14675513-14675532 MsG0280007372.01.T01:intron 35.0%
!!! TTGGTATTTTAGCCTCCTAA+TGG - Chr2:14675512-14675531 MsG0280007372.01.T01:intron 35.0%
AACAAGAACAGTACCAGCAA+CGG + Chr2:14674064-14674083 None:intergenic 40.0%
AATACACGTTCACGAGGTAT+AGG + Chr2:14675352-14675371 None:intergenic 40.0%
AATCATATGAGCCCATTAGG+AGG + Chr2:14675527-14675546 None:intergenic 40.0%
AATTAGGCAAACAACCAACG+AGG + Chr2:14675605-14675624 None:intergenic 40.0%
CTGAATCATATGAGCCCATT+AGG + Chr2:14675530-14675549 None:intergenic 40.0%
CTGTTAAGAAACAAATGCGG+GGG - Chr2:14676240-14676259 MsG0280007372.01.T01:CDS 40.0%
CTTAGCCATAAGAACCTGAT+CGG + Chr2:14674163-14674182 None:intergenic 40.0%
CTTCTTGAAGCTACACTCAT+GGG - Chr2:14674186-14674205 MsG0280007372.01.T01:CDS 40.0%
GACTCCGAAAGATCAGAAAA+TGG + Chr2:14674677-14674696 None:intergenic 40.0%
GCTAAAATACCAAGAGTGAC+CGG + Chr2:14675505-14675524 None:intergenic 40.0%
GCTGTTAAGAAACAAATGCG+GGG - Chr2:14676239-14676258 MsG0280007372.01.T01:CDS 40.0%
GTTGTACGGATGTAATACAC+CGG - Chr2:14674568-14674587 MsG0280007372.01.T01:intron 40.0%
TCTTCTTGAAGCTACACTCA+TGG - Chr2:14674185-14674204 MsG0280007372.01.T01:CDS 40.0%
TGTTAAGAAACAAATGCGGG+GGG - Chr2:14676241-14676260 MsG0280007372.01.T01:CDS 40.0%
TGTTATTCAAGACCCCATTG+AGG - Chr2:14673977-14673996 MsG0280007372.01.T01:CDS 40.0%
TTCTGTCAACAGATTGTAGC+TGG + Chr2:14676484-14676503 None:intergenic 40.0%
TTCTGTTAATCCTACCGATC+AGG - Chr2:14674146-14674165 MsG0280007372.01.T01:CDS 40.0%
! AGTTATTGGTGGCATTAGCA+GGG - Chr2:14675791-14675810 MsG0280007372.01.T01:intron 40.0%
! TTTCCTTAGAGCACTTACAC+TGG + Chr2:14676399-14676418 None:intergenic 40.0%
!! GCTCTAAGGAAACAATCTGA+AGG - Chr2:14676407-14676426 MsG0280007372.01.T01:CDS 40.0%
AAGCCAGTGTAAGTGCTCTA+AGG - Chr2:14676393-14676412 MsG0280007372.01.T01:CDS 45.0%
ACATAAGGGAACTCCGGATT+CGG - Chr2:14676201-14676220 MsG0280007372.01.T01:intron 45.0%
CAACAGATTGTAGCTGGTTG+CGG + Chr2:14676478-14676497 None:intergenic 45.0%
CGAAGCGTAGCAGAAAAATC+AGG - Chr2:14674972-14674991 MsG0280007372.01.T01:intron 45.0%
CTGTCCTCTGTTGTCAAAAG+CGG - Chr2:14674931-14674950 MsG0280007372.01.T01:intron 45.0%
GTCAATGCAGAAATACGGTG+TGG + Chr2:14675261-14675280 None:intergenic 45.0%
GTTATGGTGAAAACCGTTGC+TGG - Chr2:14674048-14674067 MsG0280007372.01.T01:CDS 45.0%
GTTCGAAATACACGTTCACG+AGG + Chr2:14675358-14675377 None:intergenic 45.0%
TCCTACCGATCAGGTTCTTA+TGG - Chr2:14674155-14674174 MsG0280007372.01.T01:CDS 45.0%
TCTAGAAGCTGAACCTAGAC+AGG + Chr2:14675293-14675312 None:intergenic 45.0%
TCTTGAGTTTACTGTGCCTC+CGG - Chr2:14674862-14674881 MsG0280007372.01.T01:intron 45.0%
TGTAATACACCGGTGTGTGT+TGG - Chr2:14674578-14674597 MsG0280007372.01.T01:intron 45.0%
! GAGTTATTGGTGGCATTAGC+AGG - Chr2:14675790-14675809 MsG0280007372.01.T01:intron 45.0%
! GCAACGGTTTTCACCATAAC+AGG + Chr2:14674048-14674067 None:intergenic 45.0%
! TCTAGTTTCCGTATCGGATC+CGG + Chr2:14674478-14674497 None:intergenic 45.0%
! TTTTGACAACAGAGGACAGG+AGG + Chr2:14674930-14674949 None:intergenic 45.0%
AAATACGGTGTGGCGCATAG+CGG + Chr2:14675251-14675270 None:intergenic 50.0%
ACTTGTGAGTGAAGCCTCGT+TGG - Chr2:14675588-14675607 MsG0280007372.01.T01:CDS 50.0%
AGAAGTGGAAAGCGGCAAAG+CGG - Chr2:14675026-14675045 MsG0280007372.01.T01:intron 50.0%
AGAGAAAGAGCCTTCACCCT+TGG - Chr2:14675474-14675493 MsG0280007372.01.T01:intron 50.0%
AGAGGCTTTGCCCTGTCAAA+CGG - Chr2:14674901-14674920 MsG0280007372.01.T01:intron 50.0%
ATAATGCTCGCAAGCCACAG+AGG + Chr2:14676112-14676131 None:intergenic 50.0%
CAACAACGTGGGATCGAAGA+TGG - Chr2:14674120-14674139 MsG0280007372.01.T01:CDS 50.0%
CCATGCTCTTTCTCCGAATC+CGG + Chr2:14676217-14676236 None:intergenic 50.0%
CGGGTATCAGAAGTGGAAAG+CGG - Chr2:14675018-14675037 MsG0280007372.01.T01:intron 50.0%
GAGAAAGAGCCTTCACCCTT+GGG - Chr2:14675475-14675494 MsG0280007372.01.T01:intron 50.0%
GCCATAAGAACCTGATCGGT+AGG + Chr2:14674159-14674178 None:intergenic 50.0%
GGCTGCCTTCAATTCTCGAA+TGG - Chr2:14674838-14674857 MsG0280007372.01.T01:intron 50.0%
TCTGCTACGCTTCGCTATAG+CGG + Chr2:14674966-14674985 None:intergenic 50.0%
TGACAACAGAGCTCCTGTCT+AGG - Chr2:14675277-14675296 MsG0280007372.01.T01:intron 50.0%
TGACACTGACACAGACACGT+TGG - Chr2:14674500-14674519 MsG0280007372.01.T01:intron 50.0%
TTGCTGCATCTGATGCAGCA+TGG - Chr2:14674817-14674836 MsG0280007372.01.T01:intron 50.0%
TTGGGTAAACCGGTCACTCT+TGG - Chr2:14675493-14675512 MsG0280007372.01.T01:intron 50.0%
TTTGACAGGGCAAAGCCTCT+CGG + Chr2:14674901-14674920 None:intergenic 50.0%
! AAGAGTGACCGGTTTACCCA+AGG + Chr2:14675494-14675513 None:intergenic 50.0%
! ACGGAGTATCTGTCAAGCAC+CGG - Chr2:14674456-14674475 MsG0280007372.01.T01:intron 50.0%
! AGAGTGACCGGTTTACCCAA+GGG + Chr2:14675493-14675512 None:intergenic 50.0%
! CGCTTTTGACAACAGAGGAC+AGG + Chr2:14674933-14674952 None:intergenic 50.0%
! GAGAGCAGAAGTTGATCGTC+TGG - Chr2:14676328-14676347 MsG0280007372.01.T01:CDS 50.0%
! GCTTTGGATCGGTTGAAACG+AGG - Chr2:14674015-14674034 MsG0280007372.01.T01:CDS 50.0%
AAAATCAGGACCACTGCGCC+AGG - Chr2:14674986-14675005 MsG0280007372.01.T01:intron 55.0%
AATACGGTGTGGCGCATAGC+GGG + Chr2:14675250-14675269 None:intergenic 55.0%
ACGAGGTCGTGGACCTGTTA+TGG - Chr2:14674032-14674051 MsG0280007372.01.T01:CDS 55.0%
CAGATTGTAGCTGGTTGCGG+AGG + Chr2:14676475-14676494 None:intergenic 55.0%
CCGGATTCGGAGAAAGAGCA+TGG - Chr2:14676214-14676233 MsG0280007372.01.T01:CDS 55.0%
CGTTCACGAGGTATAGGCAC+TGG + Chr2:14675346-14675365 None:intergenic 55.0%
GAGGCTTTGCCCTGTCAAAC+GGG - Chr2:14674902-14674921 MsG0280007372.01.T01:intron 55.0%
GATCGGTTGAAACGAGGTCG+TGG - Chr2:14674021-14674040 MsG0280007372.01.T01:CDS 55.0%
GCCTTCACCCTTGGGTAAAC+CGG - Chr2:14675483-14675502 MsG0280007372.01.T01:intron 55.0%
TCTCCGTGACCAACACACAC+CGG + Chr2:14674590-14674609 None:intergenic 55.0%
! ACACCGGTGTGTGTTGGTCA+CGG - Chr2:14674584-14674603 MsG0280007372.01.T01:intron 55.0%
! ACCGGTTTACCCAAGGGTGA+AGG + Chr2:14675487-14675506 None:intergenic 55.0%
! GTAGCTGGTTGCGGAGGTTT+TGG + Chr2:14676469-14676488 None:intergenic 55.0%
!! ACTGGAAGAGGTGCCAACAG+GGG + Chr2:14675328-14675347 None:intergenic 55.0%
!! CACTGGAAGAGGTGCCAACA+GGG + Chr2:14675329-14675348 None:intergenic 55.0%
!! CGAGGTATAGGCACTGGAAG+AGG + Chr2:14675340-14675359 None:intergenic 55.0%
!! GTCAAGCACCGGATCCGATA+CGG - Chr2:14674467-14674486 MsG0280007372.01.T01:intron 55.0%
ACAGGAGGAGCCCGTTTGAC+AGG + Chr2:14674915-14674934 None:intergenic 60.0%
ACCTCTCTCAGCTCCCCTGT+TGG - Chr2:14675312-14675331 MsG0280007372.01.T01:intron 60.0%
ACTTCTGATACCCGCCGACG+CGG + Chr2:14675012-14675031 None:intergenic 60.0%
AGGGCAAAGCCTCTCGGACT+GGG + Chr2:14674895-14674914 None:intergenic 60.0%
CAGGAGGAGCCCGTTTGACA+GGG + Chr2:14674914-14674933 None:intergenic 60.0%
CTACGCTTCGCTATAGCGGC+CGG + Chr2:14674962-14674981 None:intergenic 60.0%
GAAACAAATGCGGGGGGCTG+AGG - Chr2:14676247-14676266 MsG0280007372.01.T01:CDS 60.0%
GTTGTCAAAAGCGGCCGCAC+CGG - Chr2:14674940-14674959 MsG0280007372.01.T01:intron 60.0%
! TCTCGGACTGGGGAAAGAGC+CGG + Chr2:14674884-14674903 None:intergenic 60.0%
!! CATAGCGGGCGCTATTTCGG+TGG + Chr2:14675236-14675255 None:intergenic 60.0%
!! GCACTGGAAGAGGTGCCAAC+AGG + Chr2:14675330-14675349 None:intergenic 60.0%
!! GCGCATAGCGGGCGCTATTT+CGG + Chr2:14675239-14675258 None:intergenic 60.0%
!!! GCGGCCGCTTTTGACAACAG+AGG + Chr2:14674938-14674957 None:intergenic 60.0%
CAGGGCAAAGCCTCTCGGAC+TGG + Chr2:14674896-14674915 None:intergenic 65.0%
CTTCGCTATAGCGGCCGGTG+CGG + Chr2:14674957-14674976 None:intergenic 65.0%
GCCAACAGGGGAGCTGAGAG+AGG + Chr2:14675316-14675335 None:intergenic 65.0%
GGCTCTTTCCCCAGTCCGAG+AGG - Chr2:14674883-14674902 MsG0280007372.01.T01:intron 65.0%
GGGCAAAGCCTCTCGGACTG+GGG + Chr2:14674894-14674913 None:intergenic 65.0%
GGGGCTGAGGATGCGAAAGC+TGG - Chr2:14676260-14676279 MsG0280007372.01.T01:CDS 65.0%
! GCGTCGGCGGGTATCAGAAG+TGG - Chr2:14675011-14675030 MsG0280007372.01.T01:intron 65.0%
! CGGACTGGGGAAAGAGCCGG+AGG + Chr2:14674881-14674900 None:intergenic 70.0%
ACCACTGCGCCAGGCCGCGT+CGG - Chr2:14674995-14675014 MsG0280007372.01.T01:intron 75.0%
TGATACCCGCCGACGCGGCC+TGG + Chr2:14675007-14675026 None:intergenic 75.0%
! ACTGCGCCAGGCCGCGTCGG+CGG - Chr2:14674998-14675017 MsG0280007372.01.T01:intron 80.0%
! GCCGACGCGGCCTGGCGCAG+TGG + Chr2:14674999-14675018 None:intergenic 85.0%
!! CTGCGCCAGGCCGCGTCGGC+GGG - Chr2:14674999-14675018 MsG0280007372.01.T01:intron 85.0%
Chromosome Type Strat End Strand Name
Chr2 gene 14673922 14676538 14673922 ID=MsG0280007372.01;Name=MsG0280007372.01
Chr2 mRNA 14673922 14676538 14673922 ID=MsG0280007372.01.T01;Parent=MsG0280007372.01;Name=MsG0280007372.01.T01;_AED=0.42;_eAED=0.56;_QI=0|0|0|1|0.66|0.75|4|0|320
Chr2 exon 14676211 14676538 14676211 ID=MsG0280007372.01.T01:exon:4046;Parent=MsG0280007372.01.T01
Chr2 exon 14675901 14676009 14675901 ID=MsG0280007372.01.T01:exon:4045;Parent=MsG0280007372.01.T01
Chr2 exon 14675546 14675625 14675546 ID=MsG0280007372.01.T01:exon:4044;Parent=MsG0280007372.01.T01
Chr2 exon 14673922 14674367 14673922 ID=MsG0280007372.01.T01:exon:4043;Parent=MsG0280007372.01.T01
Chr2 CDS 14676211 14676538 14676211 ID=MsG0280007372.01.T01:cds;Parent=MsG0280007372.01.T01
Chr2 CDS 14675901 14676009 14675901 ID=MsG0280007372.01.T01:cds;Parent=MsG0280007372.01.T01
Chr2 CDS 14675546 14675625 14675546 ID=MsG0280007372.01.T01:cds;Parent=MsG0280007372.01.T01
Chr2 CDS 14673922 14674367 14673922 ID=MsG0280007372.01.T01:cds;Parent=MsG0280007372.01.T01
Gene Sequence

>MsG0280007372.01.T01

ATGATTCATCTATTATTCACAGTGATTTTTTCAGAAGCAACAATGATCGCATTGCTGTTATTCAAGACCCCATTGAGGAAGCTTGTAATAATGGCTTTGGATCGGTTGAAACGAGGTCGTGGACCTGTTATGGTGAAAACCGTTGCTGGTACTGTTCTTGTTGTTTTGTCTTCTAGTGTTTATAGTATGATGAAGATTCAACAACGTGGGATCGAAGATGGTGCTTCTGTTAATCCTACCGATCAGGTTCTTATGGCTAAGCATCTTCTTGAAGCTACACTCATGGGTATGTTTTTTTTAAATGGGTTGTTGAAAAGTTTTGATTTTTACTGGACGGAGTATCTGTCAAGCACCGGATCCGATACGGAAACTAGATATGACACTGACACAGACACGTTGGAACAAAGAACGTTTTGTATGAGTTGTGAGATACATTTCATGGCTGCCTTCAATTCTCGAATGGTTCTTGAGTTTACTGTGCCTCCGGCTCTTTCCCCAGTCCGAGAGGCTTTGCCCTACTTATTAAAAGATATCCTCTGTGGCTTGCGAGCATTATTCATTCTTGGTCAATTATTTGCAGGTGCTATCCTTTTTCTTGCACTTATGATAGACAGACTGCATCATTACATAAGGGAACTCCGGATTCGGAGAAAGAGCATGGAAGCTGTTAAGAAACAAATGCGGGGGGCTGAGGATGCGAAAGCTGGAACTTCAGATGAAACTAAAACAATGGAGCAAGAAGCAGCTAAGTTGAGAGCAGAAGTTGATCGTCTGGAATCTGAACTTCAGTCAAAAGATAAAGAGGTAGGTGCTGCAGAAGCCAGTGTAAGTGCTCTAAGGAAACAATCTGAAGGGTTTCTTCTTGAATATGATCGCTTACTTGAGGAGAACCAAAACCTCCGCAACCAGCTACAATCTGTTGACAGAAGATTCTCACATTCAGTTTCCAAGAAGAATATGTGA
Protein sequence

>MsG0280007372.01.T01

MIHLLFTVIFSEATMIALLLFKTPLRKLVIMALDRLKRGRGPVMVKTVAGTVLVVLSSSVYSMMKIQQRGIEDGASVNPTDQVLMAKHLLEATLMGMFFLNGLLKSFDFYWTEYLSSTGSDTETRYDTDTDTLEQRTFCMSCEIHFMAAFNSRMVLEFTVPPALSPVREALPYLLKDILCGLRALFILGQLFAGAILFLALMIDRLHHYIRELRIRRKSMEAVKKQMRGAEDAKAGTSDETKTMEQEAAKLRAEVDRLESELQSKDKEVGAAEASVSALRKQSEGFLLEYDRLLEENQNLRNQLQSVDRRFSHSVSKKNM*