AlfalfaGEDB | MsG0280010463.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010463.01

KEGG: -
GO: GO:0016788
PFAM: PF00657

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010463.01.T01	XP_013464883.1	79.085	153	27	4	1	150	195	345	2.02E-71	229

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010463.01.T01	Q9FFC6	68.696	115	34	1	38	150	241	355	1.08E-52	174

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010463.01.T01	A0A072VBZ2	79.085	153	27	4	1	150	195	345	9.64e-72	229

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048652.01	MsG0280010463.01	0.808903	2.463238e-50	1.425644e-47
MsG0180001079.01	MsG0280010463.01	0.818559	1.839974e-52	1.381890e-49
MsG0180001801.01	MsG0280010463.01	0.800065	1.716323e-48	7.916460e-46
MsG0180001802.01	MsG0280010463.01	0.802827	4.662581e-49	2.306953e-46
MsG0180005757.01	MsG0280010463.01	0.814382	1.585044e-51	1.061996e-48
MsG0280006485.01	MsG0280010463.01	0.829269	5.657205e-55	5.762218e-52
MsG0280008659.01	MsG0280010463.01	0.807380	5.198649e-50	2.891277e-47
MsG0280010463.01	MsG0280011267.01	0.815629	8.382641e-52	5.810160e-49
MsG0280010463.01	MsG0380016476.01	0.815024	1.142448e-51	7.788064e-49
MsG0280010463.01	MsG0480022428.01	0.813499	2.481249e-51	1.623130e-48
MsG0280010463.01	MsG0580025117.01	0.814841	1.254429e-51	8.509038e-49
MsG0280010463.01	MsG0580027036.01	0.817811	2.717105e-52	1.999152e-49
MsG0280010463.01	MsG0580028023.01	0.824336	8.528850e-54	7.532144e-51
MsG0280010463.01	MsG0580029792.01	-0.801000	1.106675e-48	5.227431e-46
MsG0280010463.01	MsG0680030961.01	0.803928	2.758636e-49	1.403924e-46
MsG0280010463.01	MsG0780037751.01	0.834101	3.641694e-56	4.278160e-53
MsG0280010463.01	MsG0780040034.01	0.801650	8.146179e-49	3.911094e-46
MsG0280010463.01	MsG0880043261.01	0.811412	7.093240e-51	4.387833e-48
MsG0280010463.01	MsG0880043989.01	0.804883	1.743978e-49	9.096901e-47
MsG0280010463.01	MsG0880045804.01	0.811652	6.290040e-51	3.915320e-48
MsG0280010463.01	MsG0880046463.01	0.848391	6.315730e-60	1.157272e-56

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010463.01.T01	MTR_2g084585	79.085	153	27	4	1	150	195	345	2.44e-75	229
MsG0280010463.01.T01	MTR_1108s0010	57.851	121	50	1	41	160	95	215	3.63e-47	153
MsG0280010463.01.T01	MTR_4g056220	51.370	146	69	2	16	160	49	193	1.40e-46	150
MsG0280010463.01.T01	MTR_4g036385	57.265	117	49	1	41	156	20	136	2.55e-46	148
MsG0280010463.01.T01	MTR_4g056280	57.025	121	51	1	41	160	236	356	2.73e-44	149
MsG0280010463.01.T01	MTR_2g100620	57.143	112	47	1	40	150	236	347	2.28e-43	147
MsG0280010463.01.T01	MTR_8g014910	58.929	112	45	1	40	150	237	348	6.78e-43	145
MsG0280010463.01.T01	MTR_4g056160	54.545	110	48	1	42	149	235	344	3.17e-36	128
MsG0280010463.01.T01	MTR_6g049230	56.667	90	39	0	41	130	105	194	1.09e-33	117
MsG0280010463.01.T01	MTR_5g084770	38.525	122	75	0	28	149	222	343	6.02e-27	103
MsG0280010463.01.T01	MTR_8g075230	44.037	109	61	0	41	149	242	350	3.53e-26	102
MsG0280010463.01.T01	MTR_1g025760	40.000	110	65	1	40	149	286	394	2.68e-25	100
MsG0280010463.01.T01	MTR_5g084750	40.179	112	67	0	38	149	233	344	3.64e-24	96.7
MsG0280010463.01.T01	MTR_3g082830	41.284	109	64	0	41	149	244	352	7.40e-24	95.9
MsG0280010463.01.T01	MTR_3g464730	40.179	112	67	0	38	149	232	343	1.22e-23	95.1
MsG0280010463.01.T01	MTR_1g025780	38.532	109	66	1	41	149	246	353	1.08e-22	92.8
MsG0280010463.01.T01	MTR_5g074210	35.507	138	85	3	22	156	78	214	2.99e-22	89.0
MsG0280010463.01.T01	MTR_5g084790	36.697	109	69	0	37	145	234	342	7.03e-22	90.5
MsG0280010463.01.T01	MTR_5g074270	36.090	133	80	3	28	156	227	358	2.43e-21	89.0
MsG0280010463.01.T01	MTR_3g114090	37.273	110	68	1	40	149	242	350	2.71e-21	89.0
MsG0280010463.01.T01	MTR_3g114120	40.952	105	61	1	45	149	257	360	3.34e-21	88.6
MsG0280010463.01.T01	MTR_5g022630	41.905	105	61	0	40	144	250	354	5.79e-21	88.2
MsG0280010463.01.T01	MTR_4g056290	60.345	58	23	0	96	153	46	103	1.56e-20	82.0
MsG0280010463.01.T01	MTR_8g075220	36.697	109	69	0	41	149	251	359	2.17e-20	86.7
MsG0280010463.01.T01	MTR_1g025720	36.190	105	66	1	41	145	243	346	5.21e-20	85.5
MsG0280010463.01.T01	MTR_5g083040	38.017	121	72	3	37	156	240	358	1.23e-19	84.3
MsG0280010463.01.T01	MTR_5g022640	32.576	132	84	2	23	149	227	358	1.68e-19	84.0
MsG0280010463.01.T01	MTR_8g075200	35.780	109	70	0	41	149	247	355	2.20e-19	84.0
MsG0280010463.01.T01	MTR_2g015080	36.607	112	66	2	33	140	242	352	3.38e-19	83.2
MsG0280010463.01.T01	MTR_3g052490	35.514	107	68	1	42	148	242	347	5.49e-19	82.8
MsG0280010463.01.T01	MTR_1g025700	34.579	107	69	1	43	149	258	363	3.10e-18	80.5
MsG0280010463.01.T01	MTR_8g062580	33.913	115	75	1	37	151	70	183	4.01e-18	78.6
MsG0280010463.01.T01	MTR_3g052580	32.710	107	71	1	42	148	237	342	2.02e-17	78.6
MsG0280010463.01.T01	MTR_3g052580	32.710	107	71	1	42	148	236	341	2.14e-17	78.6
MsG0280010463.01.T01	MTR_1g025680	39.175	97	57	2	49	144	248	343	4.64e-17	77.8
MsG0280010463.01.T01	MTR_3g435540	33.645	107	70	1	43	149	232	337	3.37e-16	74.7
MsG0280010463.01.T01	MTR_7g033165	31.544	149	88	4	15	149	215	363	1.55e-15	73.2
MsG0280010463.01.T01	MTR_7g111780	34.127	126	76	2	38	159	135	257	1.71e-15	72.0
MsG0280010463.01.T01	MTR_5g078210	36.283	113	66	3	39	149	229	337	1.82e-15	72.8
MsG0280010463.01.T01	MTR_7g111780	34.127	126	76	2	38	159	234	356	2.55e-15	72.4
MsG0280010463.01.T01	MTR_4g108690	33.645	107	70	1	43	149	236	341	3.09e-15	72.0
MsG0280010463.01.T01	MTR_5g009100	29.907	107	74	1	42	148	254	359	3.91e-15	72.0
MsG0280010463.01.T01	MTR_8g079050	31.250	128	88	0	22	149	231	358	4.10e-15	72.0
MsG0280010463.01.T01	MTR_5g093090	30.833	120	81	2	40	159	236	353	4.51e-15	71.6
MsG0280010463.01.T01	MTR_8g028160	35.821	134	79	3	33	162	247	377	5.71e-15	71.6
MsG0280010463.01.T01	MTR_5g087690	33.654	104	68	1	43	146	253	355	1.65e-14	70.1
MsG0280010463.01.T01	MTR_4g087985	34.259	108	65	2	38	140	242	348	2.05e-14	69.7
MsG0280010463.01.T01	MTR_7g008060	35.185	108	69	1	42	149	228	334	2.29e-14	69.7
MsG0280010463.01.T01	MTR_1g069175	34.259	108	70	1	42	149	243	349	4.37e-13	66.2
MsG0280010463.01.T01	MTR_1g025910	27.869	122	87	1	19	140	219	339	4.60e-13	65.9
MsG0280010463.01.T01	MTR_4g122200	33.333	129	84	2	22	149	223	350	6.16e-13	65.5
MsG0280010463.01.T01	MTR_1g079540	30.000	130	89	2	30	159	227	354	6.45e-13	65.5
MsG0280010463.01.T01	MTR_7g116570	35.043	117	74	2	43	159	243	357	7.23e-13	65.5
MsG0280010463.01.T01	MTR_3g098270	31.731	104	70	1	42	145	245	347	8.65e-13	65.5
MsG0280010463.01.T01	MTR_2g033920	33.628	113	73	2	38	149	239	350	9.72e-13	65.1
MsG0280010463.01.T01	MTR_3g024510	27.027	148	102	3	1	145	203	347	1.09e-12	65.1
MsG0280010463.01.T01	MTR_0565s0010	30.476	105	72	1	41	145	239	342	1.29e-12	64.7
MsG0280010463.01.T01	MTR_7g033155	26.446	121	81	1	37	149	263	383	1.74e-12	64.3
MsG0280010463.01.T01	MTR_1g030275	33.051	118	70	2	41	149	249	366	1.78e-12	64.3
MsG0280010463.01.T01	MTR_8g087890	32.039	103	69	1	43	145	239	340	1.88e-12	64.3
MsG0280010463.01.T01	MTR_7g033150	28.099	121	79	2	37	149	249	369	3.02e-12	63.5
MsG0280010463.01.T01	MTR_8g022790	32.407	108	65	2	39	145	239	339	3.51e-12	63.5
MsG0280010463.01.T01	MTR_7g116510	32.407	108	72	1	42	149	234	340	4.75e-12	63.2
MsG0280010463.01.T01	MTR_1g030265	28.472	144	89	2	19	148	190	333	6.45e-12	62.8
MsG0280010463.01.T01	MTR_4g035353	30.769	104	71	1	42	145	239	341	7.36e-12	62.4
MsG0280010463.01.T01	MTR_8g031420	30.556	108	69	2	43	145	235	341	8.76e-12	62.4
MsG0280010463.01.T01	MTR_7g116870	28.369	141	86	3	33	161	232	369	1.03e-11	62.0
MsG0280010463.01.T01	MTR_3g052480	29.091	110	72	3	42	148	243	349	1.09e-11	62.0
MsG0280010463.01.T01	MTR_7g116500	31.667	120	77	2	38	153	233	351	1.23e-11	62.0
MsG0280010463.01.T01	MTR_1g030340	29.032	155	96	4	19	159	228	382	1.46e-11	61.6
MsG0280010463.01.T01	MTR_7g116520	34.579	107	69	1	43	149	243	348	1.60e-11	61.6
MsG0280010463.01.T01	MTR_5g093550	41.892	74	37	3	88	156	70	142	1.63e-11	59.3
MsG0280010463.01.T01	MTR_1g030268	32.479	117	70	2	41	148	250	366	1.89e-11	61.6
MsG0280010463.01.T01	MTR_6g007095	29.936	157	95	7	19	163	224	377	2.55e-11	61.2
MsG0280010463.01.T01	MTR_6g007095	28.169	142	93	4	19	157	553	688	6.57e-11	60.1
MsG0280010463.01.T01	MTR_2g015580	32.110	109	71	2	43	149	266	373	3.54e-11	60.8
MsG0280010463.01.T01	MTR_4g019880	41.538	65	37	1	85	149	285	348	5.35e-11	60.1
MsG0280010463.01.T01	MTR_2g033930	32.743	113	74	2	38	149	234	345	5.93e-11	60.1
MsG0280010463.01.T01	MTR_8g074570	28.704	108	76	1	43	149	247	354	7.22e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010463.01.T01	AT5G22810	68.696	115	34	1	38	150	238	352	1.28e-53	174
MsG0280010463.01.T01	AT5G03820	63.636	110	40	0	41	150	241	350	2.95e-48	160
MsG0280010463.01.T01	AT5G03820	63.636	110	40	0	41	150	269	378	5.51e-48	160
MsG0280010463.01.T01	AT5G03810	59.350	123	50	0	28	150	224	346	2.02e-47	157
MsG0280010463.01.T01	AT3G53100	59.633	109	44	0	41	149	235	343	3.47e-43	146
MsG0280010463.01.T01	AT3G16370	56.098	123	52	2	28	149	224	345	7.74e-42	143
MsG0280010463.01.T01	AT1G75880	44.954	109	60	0	41	149	259	367	8.79e-28	106
MsG0280010463.01.T01	AT1G75880	44.954	109	60	0	41	149	258	366	9.52e-28	106
MsG0280010463.01.T01	AT1G20120	40.909	110	65	0	40	149	283	392	6.08e-26	102
MsG0280010463.01.T01	AT5G42170	39.091	110	67	0	40	149	252	361	1.85e-25	100
MsG0280010463.01.T01	AT3G14820	43.636	110	61	1	40	149	234	342	7.57e-25	98.6
MsG0280010463.01.T01	AT1G75890	40.952	105	62	0	41	145	267	371	5.44e-24	96.7
MsG0280010463.01.T01	AT1G75890	40.952	105	62	0	41	145	283	387	6.19e-24	96.7
MsG0280010463.01.T01	AT1G75890	40.952	105	62	0	41	145	254	358	6.33e-24	96.3
MsG0280010463.01.T01	AT1G75900	39.450	109	66	0	41	149	248	356	8.87e-24	95.9
MsG0280010463.01.T01	AT2G04570	37.963	108	67	0	42	149	235	342	2.49e-23	94.4
MsG0280010463.01.T01	AT2G30220	36.842	133	77	2	20	146	215	346	8.05e-23	93.2
MsG0280010463.01.T01	AT1G59406	41.121	107	62	1	43	149	236	341	2.91e-22	91.7
MsG0280010463.01.T01	AT1G59030	41.121	107	62	1	43	149	236	341	2.91e-22	91.7
MsG0280010463.01.T01	AT1G58725	41.121	107	62	1	43	149	236	341	2.91e-22	91.7
MsG0280010463.01.T01	AT2G30310	35.433	127	75	2	20	140	216	341	5.31e-22	90.9
MsG0280010463.01.T01	AT2G42990	37.500	104	65	0	43	146	236	339	2.63e-21	89.0
MsG0280010463.01.T01	AT2G31550	34.906	106	68	1	43	148	105	209	3.22e-21	86.3
MsG0280010463.01.T01	AT4G26790	37.037	108	68	0	42	149	236	343	4.38e-21	88.2
MsG0280010463.01.T01	AT4G26790	37.037	108	68	0	42	149	236	343	4.38e-21	88.2
MsG0280010463.01.T01	AT1G06990	35.714	140	76	4	20	149	217	352	9.86e-21	87.4
MsG0280010463.01.T01	AT1G20130	38.739	111	64	1	43	149	399	509	2.24e-20	87.4
MsG0280010463.01.T01	AT2G31540	34.906	106	68	1	43	148	246	350	1.26e-19	84.3
MsG0280010463.01.T01	AT1G20130	38.739	111	64	1	43	149	419	529	1.26e-19	85.1
MsG0280010463.01.T01	AT1G20132	34.545	110	72	0	40	149	266	375	1.27e-19	84.7
MsG0280010463.01.T01	AT2G24560	37.113	97	60	1	43	139	245	340	3.84e-19	83.2
MsG0280010463.01.T01	AT2G40250	35.772	123	72	2	23	145	235	350	4.40e-19	82.8
MsG0280010463.01.T01	AT1G58430	34.906	106	68	1	43	148	246	350	5.29e-19	82.8
MsG0280010463.01.T01	AT1G58520	39.252	107	57	2	43	149	236	334	5.34e-19	82.4
MsG0280010463.01.T01	AT1G58525	39.252	107	57	2	43	149	236	334	5.34e-19	82.4
MsG0280010463.01.T01	AT5G45960	34.559	136	75	4	16	138	220	354	1.01e-18	82.0
MsG0280010463.01.T01	AT2G23540	34.375	128	84	0	22	149	242	369	3.69e-17	77.8
MsG0280010463.01.T01	AT3G43570	42.466	73	42	0	77	149	240	312	6.42e-17	76.6
MsG0280010463.01.T01	AT5G14450	34.559	136	77	3	22	149	240	371	1.07e-16	76.6
MsG0280010463.01.T01	AT5G37690	37.615	109	62	3	43	149	227	331	1.60e-16	75.9
MsG0280010463.01.T01	AT5G37690	37.615	109	62	3	43	149	235	339	1.76e-16	75.5
MsG0280010463.01.T01	AT5G45950	36.207	116	69	4	40	155	242	352	4.49e-16	74.7
MsG0280010463.01.T01	AT5G15720	32.759	116	76	2	43	157	210	324	4.90e-16	74.3
MsG0280010463.01.T01	AT5G15720	32.759	116	76	2	43	157	241	355	5.08e-16	74.3
MsG0280010463.01.T01	AT1G29660	35.922	103	65	1	43	145	240	341	8.08e-16	73.9
MsG0280010463.01.T01	AT4G18970	29.012	162	109	3	1	159	194	352	5.19e-15	71.6
MsG0280010463.01.T01	AT4G18970	29.012	162	109	3	1	159	194	352	5.90e-15	71.6
MsG0280010463.01.T01	AT1G29670	32.800	125	81	2	23	145	217	340	7.90e-15	71.2
MsG0280010463.01.T01	AT1G29670	32.800	125	81	2	23	145	285	408	1.30e-14	70.5
MsG0280010463.01.T01	AT1G33811	30.323	155	102	3	4	157	212	361	1.52e-14	70.5
MsG0280010463.01.T01	AT1G09390	28.947	152	101	3	15	159	212	363	2.76e-14	69.7
MsG0280010463.01.T01	AT5G45670	33.333	105	69	1	41	145	236	339	3.40e-14	69.3
MsG0280010463.01.T01	AT3G50400	31.008	129	88	1	22	149	228	356	7.34e-14	68.6
MsG0280010463.01.T01	AT1G56670	29.605	152	100	3	15	159	215	366	7.39e-14	68.2
MsG0280010463.01.T01	AT1G71691	34.615	104	67	1	42	145	158	260	8.59e-14	67.8
MsG0280010463.01.T01	AT1G71691	34.615	104	67	1	42	145	259	361	1.17e-13	67.8
MsG0280010463.01.T01	AT4G16233	30.088	113	77	2	38	149	1	112	3.15e-13	63.5
MsG0280010463.01.T01	AT1G23500	31.776	107	63	1	43	149	241	337	3.95e-13	66.2
MsG0280010463.01.T01	AT1G71250	32.692	104	69	1	42	145	249	351	4.35e-13	66.2
MsG0280010463.01.T01	AT4G10950	28.030	132	89	4	22	148	254	384	4.79e-13	66.2
MsG0280010463.01.T01	AT4G10950	28.030	132	89	4	22	148	250	380	4.81e-13	66.2
MsG0280010463.01.T01	AT1G28660	30.894	123	78	3	43	161	252	371	6.11e-13	65.9
MsG0280010463.01.T01	AT1G28660	30.894	123	78	3	43	161	251	370	6.16e-13	65.9
MsG0280010463.01.T01	AT3G04290	31.897	116	74	2	38	149	229	343	1.11e-12	65.1
MsG0280010463.01.T01	AT5G55050	31.452	124	81	4	41	162	249	370	1.85e-12	64.3
MsG0280010463.01.T01	AT1G28640	30.081	123	79	3	43	161	252	371	2.66e-12	63.9
MsG0280010463.01.T01	AT1G28650	30.894	123	78	3	43	161	254	373	5.51e-12	63.2
MsG0280010463.01.T01	AT1G53990	31.959	97	61	1	55	146	247	343	9.71e-12	62.4
MsG0280010463.01.T01	AT3G48460	30.000	110	74	1	43	149	252	361	1.01e-11	62.4
MsG0280010463.01.T01	AT4G01130	32.394	142	83	4	28	157	146	286	1.23e-11	61.6
MsG0280010463.01.T01	AT3G27950	29.688	128	81	2	41	160	240	366	1.23e-11	62.0
MsG0280010463.01.T01	AT1G74460	29.907	107	74	1	43	149	232	337	1.50e-11	61.6
MsG0280010463.01.T01	AT4G01130	32.394	142	83	4	28	157	232	372	1.52e-11	61.6
MsG0280010463.01.T01	AT5G18430	30.952	126	85	2	39	164	235	358	2.58e-11	61.2
MsG0280010463.01.T01	AT1G75930	34.286	105	57	2	47	149	241	335	2.59e-11	60.8
MsG0280010463.01.T01	AT1G28580	28.346	127	83	3	31	149	157	283	3.66e-11	60.5
MsG0280010463.01.T01	AT5G63170	34.694	98	54	1	43	140	234	321	3.92e-11	60.5
MsG0280010463.01.T01	AT5G33370	31.481	108	73	1	42	149	238	344	4.63e-11	60.5
MsG0280010463.01.T01	AT1G28580	28.346	127	83	3	31	149	238	364	4.71e-11	60.5
MsG0280010463.01.T01	AT5G40990	34.021	97	59	1	55	146	256	352	5.07e-11	60.1
MsG0280010463.01.T01	AT1G54790	25.882	170	106	4	9	161	204	370	5.43e-11	60.1
MsG0280010463.01.T01	AT1G54790	25.882	170	106	4	9	161	205	371	6.86e-11	59.7
MsG0280010463.01.T01	AT4G28780	27.966	118	83	2	42	159	239	354	7.96e-11	59.7
MsG0280010463.01.T01	AT1G71120	25.000	108	81	0	41	148	232	339	9.04e-11	59.3
MsG0280010463.01.T01	AT5G08460	32.710	107	68	2	42	145	258	363	9.17e-11	59.7

Find 41 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGCAACTGAATATGTATTT+TGG	0.133885	2:-69981178	MsG0280010463.01.T01:CDS
GGCTGATGATTTGCTAATTC+TGG	0.158898	2:-69981117	MsG0280010463.01.T01:CDS
TCCCTCTGAGGCTGCAAATA+AGG	0.251844	2:-69981144	MsG0280010463.01.T01:CDS
CTGCTGTTGTTAAATGTGTT+TGG	0.294917	2:-69981011	MsG0280010463.01.T01:three_prime_UTR
CATTGGTCTTATTACAATAA+TGG	0.295298	2:+69980937	None:intergenic
ATGTGTTTGGCTCATGTATA+AGG	0.303376	2:-69980998	MsG0280010463.01.T01:three_prime_UTR
GTCACTAAGCCTACAGAAAA+TGG	0.316924	2:-69981408	MsG0280010463.01.T01:intron
CCAGCAGCCATCACTCTCTT+TGG	0.324207	2:-69981578	MsG0280010463.01.T01:CDS
TGAGGCTGCAAATAAGGTTT+TGG	0.333933	2:-69981138	MsG0280010463.01.T01:CDS
TGCACTTGGGGCAAGGAAAT+TGG	0.354231	2:-69981632	MsG0280010463.01.T01:CDS
AGGAAGGCTTGTTGTGGAAC+TGG	0.380493	2:-69981254	MsG0280010463.01.T01:CDS
AACGTCTATATTGTGCAATA+AGG	0.437192	2:-69981222	MsG0280010463.01.T01:CDS
TGCATTGGCACACGTTCCTA+TGG	0.447027	2:+69981196	None:intergenic
TGTGACAACATTACCTCCAT+TGG	0.451988	2:-69981609	MsG0280010463.01.T01:CDS
GGAGAGGTTGGTAGATATCA+AGG	0.495758	2:+69981439	None:intergenic
CCAAAGAGAGTGATGGCTGC+TGG	0.506853	2:+69981578	None:intergenic
AAATACATATTCAGTTGCAT+TGG	0.517531	2:+69981181	None:intergenic
ACCTTATTTGCAGCCTCAGA+GGG	0.518564	2:+69981143	None:intergenic
AGAGAGTGATGGCTGCTGGC+AGG	0.519776	2:+69981582	None:intergenic
GTGACAACATTACCTCCATT+GGG	0.522286	2:-69981608	MsG0280010463.01.T01:CDS
TGACAGAATTTGTATGCACT+TGG	0.530747	2:-69981646	MsG0280010463.01.T01:intron
TGACAAGGTCATGGAGAGGT+TGG	0.538183	2:+69981427	None:intergenic
AAAATAATATATAAAATGCT+AGG	0.539742	2:+69981058	None:intergenic
CACAGCATGAATGTGTGGAC+AGG	0.541400	2:-69981551	MsG0280010463.01.T01:CDS
ATTTGTATGCACTTGGGGCA+AGG	0.542893	2:-69981639	MsG0280010463.01.T01:CDS
TAGGCTTAGTGACAAGGTCA+TGG	0.552443	2:+69981418	None:intergenic
CAGAAAGCTCTCAAGAACAT+TGG	0.554355	2:+69980920	None:intergenic
TTTCTGTAGGCTTAGTGACA+AGG	0.555785	2:+69981412	None:intergenic
GCTGCTGGCAGGCAACCCAA+TGG	0.573362	2:+69981593	None:intergenic
GAAGCAAGGAAGGCTTGTTG+TGG	0.578628	2:-69981260	MsG0280010463.01.T01:CDS
AACCTTATTTGCAGCCTCAG+AGG	0.590083	2:+69981142	None:intergenic
TTGTGCAATAAGGACTCCAT+AGG	0.590945	2:-69981212	MsG0280010463.01.T01:CDS
GACAGAATTTGTATGCACTT+GGG	0.603577	2:-69981645	MsG0280010463.01.T01:intron
GATTAATTATAAAGTTCACT+AGG	0.606294	2:-69980964	MsG0280010463.01.T01:three_prime_UTR
ACAGAATTTGTATGCACTTG+GGG	0.615606	2:-69981644	MsG0280010463.01.T01:intron
GCTGGCAGGCAACCCAATGG+AGG	0.616284	2:+69981596	None:intergenic
AGTGCTGAGTAACCTCACAT+TGG	0.660656	2:-69981466	MsG0280010463.01.T01:CDS
TATCAAGGACAGCCAATGTG+AGG	0.673893	2:+69981454	None:intergenic
GTGAGAGCCAAAGAGAGTGA+TGG	0.684894	2:+69981571	None:intergenic
GCTCTCACAGCATGAATGTG+TGG	0.685847	2:-69981556	MsG0280010463.01.T01:CDS
TTAGTGACAAGGTCATGGAG+AGG	0.721177	2:+69981423	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATAATATATAAAATGCT+AGG	+	Chr2:69981835-69981854	None:intergenic	10.0%
!!!	AAACTAAATATTTGTTTTAA+TGG	+	Chr2:69980971-69980990	None:intergenic	10.0%
!!!	ATATAATATATTACTTGTTA+TGG	-	Chr2:69981539-69981558	MsG0280010463.01.T01:CDS	10.0%
!!!	TATATATTTTTTTTAATTGG+TGG	+	Chr2:69981172-69981191	None:intergenic	10.0%
!!!	ATACTGATTTACATTTTATT+TGG	+	Chr2:69981011-69981030	None:intergenic	15.0%
!!!	CAAATATTTAGTTTTTAAGT+AGG	-	Chr2:69980977-69980996	MsG0280010463.01.T01:three_prime_UTR	15.0%
!!	AAGTCCTAAATTTAACTATT+TGG	-	Chr2:69981101-69981120	MsG0280010463.01.T01:CDS	20.0%
!!	AGTCCTAAATTTAACTATTT+GGG	-	Chr2:69981102-69981121	MsG0280010463.01.T01:CDS	20.0%
!!	GATTAATTATAAAGTTCACT+AGG	-	Chr2:69981926-69981945	MsG0280010463.01.T01:intron	20.0%
!!!	ATATTTGTTTTAATGGTACT+AGG	+	Chr2:69980964-69980983	None:intergenic	20.0%
!!!	GATTTACATTTTATTTGGTT+TGG	+	Chr2:69981006-69981025	None:intergenic	20.0%
!	AAATACATATTCAGTTGCAT+TGG	+	Chr2:69981712-69981731	None:intergenic	25.0%
!	AGACCCAAATAGTTAAATTT+AGG	+	Chr2:69981108-69981127	None:intergenic	25.0%
!	AGGACTTCATTTAAAAGTTT+AGG	+	Chr2:69981088-69981107	None:intergenic	25.0%
!	GACATTTCTAAATTCCAAAA+TGG	-	Chr2:69981218-69981237	MsG0280010463.01.T01:CDS	25.0%
!	GGACTTCATTTAAAAGTTTA+GGG	+	Chr2:69981087-69981106	None:intergenic	25.0%
!	TATTTGGGTCTCTAAATTTA+AGG	-	Chr2:69981117-69981136	MsG0280010463.01.T01:CDS	25.0%
!!	AACACTATACATTTTCATTC+AGG	-	Chr2:69980924-69980943	MsG0280010463.01.T01:three_prime_UTR	25.0%
!!	ATATCCTAATCCAACATTTT+TGG	-	Chr2:69981806-69981825	MsG0280010463.01.T01:intron	25.0%
!!	ATGCAACTGAATATGTATTT+TGG	-	Chr2:69981712-69981731	MsG0280010463.01.T01:intron	25.0%
!!	TATCCTAATCCAACATTTTT+GGG	-	Chr2:69981807-69981826	MsG0280010463.01.T01:intron	25.0%
!!	TGCAACTGAATATGTATTTT+GGG	-	Chr2:69981713-69981732	MsG0280010463.01.T01:intron	25.0%
!!!	CATTGGTCTTATTACAATAA+TGG	+	Chr2:69981956-69981975	None:intergenic	25.0%
!!!	TTACATTTTATTTGGTTTGG+TGG	+	Chr2:69981003-69981022	None:intergenic	25.0%
	AACGTCTATATTGTGCAATA+AGG	-	Chr2:69981668-69981687	MsG0280010463.01.T01:intron	30.0%
!	AAATTCTGTCACATCCATTT+TGG	+	Chr2:69981235-69981254	None:intergenic	30.0%
!	AGTAGTTTACCATTTTCTGT+AGG	+	Chr2:69981494-69981513	None:intergenic	30.0%
!!	ACTGAATATGTATTTTGGGA+CGG	-	Chr2:69981717-69981736	MsG0280010463.01.T01:intron	30.0%
!!	TTACTTTTAAGTGTTGTGTG+TGG	-	Chr2:69981853-69981872	MsG0280010463.01.T01:intron	30.0%
!!!	ATACACTACTTTTTTCTCAC+AGG	-	Chr2:69981600-69981619	MsG0280010463.01.T01:CDS	30.0%
!!!	TACACTACTTTTTTCTCACA+GGG	-	Chr2:69981601-69981620	MsG0280010463.01.T01:CDS	30.0%
	AAAGTAGTGTATGCAACAGA+AGG	+	Chr2:69981592-69981611	None:intergenic	35.0%
	ACAGAATTTGTATGCACTTG+GGG	-	Chr2:69981246-69981265	MsG0280010463.01.T01:CDS	35.0%
	CTGCTGTTGTTAAATGTGTT+TGG	-	Chr2:69981879-69981898	MsG0280010463.01.T01:intron	35.0%
	GAACCCAAAAATGTTGGATT+AGG	+	Chr2:69981813-69981832	None:intergenic	35.0%
	GACAGAATTTGTATGCACTT+GGG	-	Chr2:69981245-69981264	MsG0280010463.01.T01:CDS	35.0%
	TGACAGAATTTGTATGCACT+TGG	-	Chr2:69981244-69981263	MsG0280010463.01.T01:CDS	35.0%
!	ATGTGTTTGGCTCATGTATA+AGG	-	Chr2:69981892-69981911	MsG0280010463.01.T01:intron	35.0%
!	TTTTTGCAAGTTCTGTGATG+TGG	+	Chr2:69981405-69981424	None:intergenic	35.0%
	CAGAAAGCTCTCAAGAACAT+TGG	+	Chr2:69981973-69981992	None:intergenic	40.0%
	GTCACTAAGCCTACAGAAAA+TGG	-	Chr2:69981482-69981501	MsG0280010463.01.T01:CDS	40.0%
	GTGACAACATTACCTCCATT+GGG	-	Chr2:69981282-69981301	MsG0280010463.01.T01:intron	40.0%
	TGCTAGGAACCCAAAAATGT+TGG	+	Chr2:69981819-69981838	None:intergenic	40.0%
	TGTGACAACATTACCTCCAT+TGG	-	Chr2:69981281-69981300	MsG0280010463.01.T01:intron	40.0%
	TTGTGCAATAAGGACTCCAT+AGG	-	Chr2:69981678-69981697	MsG0280010463.01.T01:intron	40.0%
	TTTCTGTAGGCTTAGTGACA+AGG	+	Chr2:69981481-69981500	None:intergenic	40.0%
!	TGAGGCTGCAAATAAGGTTT+TGG	-	Chr2:69981752-69981771	MsG0280010463.01.T01:intron	40.0%
!!	AGGGTTTTATGAAGCAAGGA+AGG	-	Chr2:69981620-69981639	MsG0280010463.01.T01:CDS	40.0%
!!	GGCTGATGATTTGCTAATTC+TGG	-	Chr2:69981773-69981792	MsG0280010463.01.T01:intron	40.0%
!!	TCACAGGGTTTTATGAAGCA+AGG	-	Chr2:69981616-69981635	MsG0280010463.01.T01:CDS	40.0%
	ATTTGTATGCACTTGGGGCA+AGG	-	Chr2:69981251-69981270	MsG0280010463.01.T01:CDS	45.0%
	TAGGCTTAGTGACAAGGTCA+TGG	+	Chr2:69981475-69981494	None:intergenic	45.0%
	TATCAAGGACAGCCAATGTG+AGG	+	Chr2:69981439-69981458	None:intergenic	45.0%
	TTAGTGACAAGGTCATGGAG+AGG	+	Chr2:69981470-69981489	None:intergenic	45.0%
!	AACCTTATTTGCAGCCTCAG+AGG	+	Chr2:69981751-69981770	None:intergenic	45.0%
!	ACCTTATTTGCAGCCTCAGA+GGG	+	Chr2:69981750-69981769	None:intergenic	45.0%
!	AGTGCTGAGTAACCTCACAT+TGG	-	Chr2:69981424-69981443	MsG0280010463.01.T01:CDS	45.0%
!	CTTGTTATGGCTCATGAGCA+AGG	-	Chr2:69981552-69981571	MsG0280010463.01.T01:CDS	45.0%
!!	GGAGAGGTTGGTAGATATCA+AGG	+	Chr2:69981454-69981473	None:intergenic	45.0%
!!!	AACTATATATTTTTTTTAAT+TGG	+	Chr2:69981175-69981194	None:intergenic	5.0%
	CACAGCATGAATGTGTGGAC+AGG	-	Chr2:69981339-69981358	MsG0280010463.01.T01:intron	50.0%
	GCTCTCACAGCATGAATGTG+TGG	-	Chr2:69981334-69981353	MsG0280010463.01.T01:intron	50.0%
	GTGAGAGCCAAAGAGAGTGA+TGG	+	Chr2:69981322-69981341	None:intergenic	50.0%
	TGACAAGGTCATGGAGAGGT+TGG	+	Chr2:69981466-69981485	None:intergenic	50.0%
	TGCACTTGGGGCAAGGAAAT+TGG	-	Chr2:69981258-69981277	MsG0280010463.01.T01:CDS	50.0%
!	TGCATTGGCACACGTTCCTA+TGG	+	Chr2:69981697-69981716	None:intergenic	50.0%
!!	AGGAAGGCTTGTTGTGGAAC+TGG	-	Chr2:69981636-69981655	MsG0280010463.01.T01:CDS	50.0%
!!	GAAGCAAGGAAGGCTTGTTG+TGG	-	Chr2:69981630-69981649	MsG0280010463.01.T01:CDS	50.0%
!!	TCCCTCTGAGGCTGCAAATA+AGG	-	Chr2:69981746-69981765	MsG0280010463.01.T01:intron	50.0%
	CCAAAGAGAGTGATGGCTGC+TGG	+	Chr2:69981315-69981334	None:intergenic	55.0%
	CCAGCAGCCATCACTCTCTT+TGG	-	Chr2:69981312-69981331	MsG0280010463.01.T01:intron	55.0%
!	GGACGGTTTTCATCCCTCTG+AGG	-	Chr2:69981734-69981753	MsG0280010463.01.T01:intron	55.0%
	AGAGAGTGATGGCTGCTGGC+AGG	+	Chr2:69981311-69981330	None:intergenic	60.0%
	GCTGCTGGCAGGCAACCCAA+TGG	+	Chr2:69981300-69981319	None:intergenic	65.0%
	GCTGGCAGGCAACCCAATGG+AGG	+	Chr2:69981297-69981316	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	69980920	69981992	69980920	ID=MsG0280010463.01;Name=MsG0280010463.01
Chr2	mRNA	69980920	69981992	69980920	ID=MsG0280010463.01.T01;Parent=MsG0280010463.01;Name=MsG0280010463.01.T01;_AED=0.13;_eAED=0.13;_QI=0\|1\|1\|1\|0.25\|0.2\|5\|156\|164
Chr2	exon	69980920	69981290	69980920	ID=MsG0280010463.01.T01:exon:26831;Parent=MsG0280010463.01.T01
Chr2	exon	69981409	69981662	69981409	ID=MsG0280010463.01.T01:exon:26830;Parent=MsG0280010463.01.T01
Chr2	exon	69981967	69981992	69981967	ID=MsG0280010463.01.T01:exon:26829;Parent=MsG0280010463.01.T01
Chr2	CDS	69981967	69981992	69981967	ID=MsG0280010463.01.T01:cds;Parent=MsG0280010463.01.T01
Chr2	CDS	69981409	69981662	69981409	ID=MsG0280010463.01.T01:cds;Parent=MsG0280010463.01.T01
Chr2	CDS	69981076	69981290	69981076	ID=MsG0280010463.01.T01:cds;Parent=MsG0280010463.01.T01
Chr2	three_prime_UTR	69980920	69981075	69980920	ID=MsG0280010463.01.T01:three_prime_utr;Parent=MsG0280010463.01.T01

Gene Sequence

>MsG0280010463.01.T01

ATGCAACACTATACATTTTCATTCAGAATTTGTATGCACTTGGGGCAAGGAAATTGGTGTGACAACATTACCTCCATTGGGTTGCCTGCCAGCAGCCATCACTCTCTTTGGCTCTCACAGCATGAATGTGTGGACAGGCTAAACAATGATGCTCTTAACTTCAACACTAAGCTAAACACCACATCACAGAACTTGCAAAAAGTGCTGAGTAACCTCACATTGGCTGTCCTTGATATCTACCAACCTCTCCATGACCTTGTCACTAAGCCTACAGAAAATGGGTTTTATGAAGCAAGGAAGGCTTGTTGTGGAACTGGCTTGATAGAAACGTCTATATTGTGCAATAAGGACTCCATAGGAACGTGTGCCAATGCAACTGAATATGTATTTTGGGACGGTTTTCATCCCTCTGAGGCTGCAAATAAGGTTTTGGCTGATGATTTGCTAATTCTGGCATCTCTCTCATATCCTAATCCAACATTTTTGGGTTCCTAG

Protein sequence

>MsG0280010463.01.T01

MQHYTFSFRICMHLGQGNWCDNITSIGLPASSHHSLWLSQHECVDRLNNDALNFNTKLNTTSQNLQKVLSNLTLAVLDIYQPLHDLVTKPTENGFYEARKACCGTGLIETSILCNKDSIGTCANATEYVFWDGFHPSEAANKVLADDLLILASLSYPNPTFLGS*