AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043989.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043989.01.T01 MTR_8g045695 96.795 156 5 0 1 156 1 156 1.58e-111 313
MsG0880043989.01.T01 MTR_8g045735 88.961 154 17 0 1 154 1 154 4.93e-102 289
MsG0880043989.01.T01 MTR_8g045665 87.821 156 19 0 1 156 1 156 2.05e-100 285
MsG0880043989.01.T01 MTR_8g045640 83.974 156 25 0 1 156 1 156 2.23e-96 275
MsG0880043989.01.T01 MTR_8g045560 83.333 156 26 0 1 156 1 156 4.65e-95 271
MsG0880043989.01.T01 MTR_8g045520 78.344 157 33 1 1 156 1 157 6.07e-89 256
MsG0880043989.01.T01 MTR_8g045490 74.522 157 38 2 1 156 1 156 4.98e-85 246
MsG0880043989.01.T01 MTR_8g045400 72.611 157 41 2 1 156 1 156 1.37e-82 240
MsG0880043989.01.T01 MTR_8g045570 71.711 152 42 1 1 151 1 152 6.32e-81 236
MsG0880043989.01.T01 MTR_8g045555 70.395 152 44 1 1 151 1 152 3.10e-79 231
MsG0880043989.01.T01 MTR_8g045300 67.308 156 50 1 1 155 1 156 3.69e-78 229
MsG0880043989.01.T01 MTR_1g030810 43.137 153 83 3 1 150 1 152 1.27e-39 131
MsG0880043989.01.T01 MTR_1g030820 38.562 153 90 3 1 150 1 152 1.16e-37 126
MsG0880043989.01.T01 MTR_1g030840 38.462 156 87 5 1 148 1 155 5.64e-29 104
MsG0880043989.01.T01 MTR_8g012550 34.000 150 95 4 6 153 8 155 1.08e-26 98.6
MsG0880043989.01.T01 MTR_1g031640 38.514 148 81 6 7 151 7 147 1.22e-22 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043989.01.T01 AT5G28010 35.948 153 94 4 3 152 15 166 1.38e-25 96.3
MsG0880043989.01.T01 AT5G28010 35.948 153 94 4 3 152 15 166 1.38e-25 96.3
MsG0880043989.01.T01 AT1G70840 35.294 153 95 4 3 152 20 171 2.03e-24 93.2
MsG0880043989.01.T01 AT1G70890 35.948 153 94 4 3 152 7 158 6.00e-24 91.7
MsG0880043989.01.T01 AT1G70830 35.948 153 94 4 3 152 22 173 1.75e-23 90.9
MsG0880043989.01.T01 AT1G70830 35.714 154 95 4 3 153 22 174 2.43e-23 91.3
MsG0880043989.01.T01 AT1G70830 35.714 154 95 4 3 153 22 174 1.13e-22 90.5
MsG0880043989.01.T01 AT5G28000 33.333 153 98 4 3 152 13 164 1.22e-22 88.6
MsG0880043989.01.T01 AT1G70830 35.948 153 94 4 3 152 137 288 1.98e-22 90.9
MsG0880043989.01.T01 AT1G70830 32.990 97 63 2 59 153 31 127 3.76e-11 60.1
MsG0880043989.01.T01 AT1G70830 35.714 154 95 4 3 153 22 174 2.40e-22 91.3
MsG0880043989.01.T01 AT1G70830 35.948 153 94 4 3 152 184 335 5.93e-22 90.1
MsG0880043989.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 1.97e-21 87.0
MsG0880043989.01.T01 AT1G70850 36.364 143 87 4 13 152 175 316 3.64e-21 87.8
MsG0880043989.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 5.05e-21 87.4
MsG0880043989.01.T01 AT1G70850 36.364 143 87 4 13 152 175 316 3.64e-21 87.8
MsG0880043989.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 5.05e-21 87.4
MsG0880043989.01.T01 AT4G23670 33.333 153 98 4 1 151 1 151 6.29e-21 83.6
MsG0880043989.01.T01 AT1G14930 29.936 157 106 4 1 155 1 155 8.25e-20 80.9
MsG0880043989.01.T01 AT2G01520 30.065 153 103 4 1 151 1 151 2.99e-19 79.3
MsG0880043989.01.T01 AT1G70870 41.667 96 54 2 56 150 44 138 1.71e-18 77.0
MsG0880043989.01.T01 AT1G70880 30.065 153 103 4 3 152 8 159 1.61e-17 75.1
MsG0880043989.01.T01 AT1G14940 29.299 157 107 4 1 155 1 155 2.11e-17 74.7
MsG0880043989.01.T01 AT2G01530 30.065 153 103 4 1 151 1 151 3.86e-17 73.9
MsG0880043989.01.T01 AT4G14060 29.412 153 104 4 1 151 1 151 6.43e-17 73.2
MsG0880043989.01.T01 AT4G23680 29.605 152 103 4 1 150 1 150 1.11e-16 72.8
MsG0880043989.01.T01 AT1G24020 31.613 155 97 4 1 150 1 151 1.47e-16 72.4
MsG0880043989.01.T01 AT1G24020 31.613 155 97 4 1 150 1 151 1.47e-16 72.4
MsG0880043989.01.T01 AT1G23130 28.188 149 104 3 3 149 10 157 3.16e-16 71.6
MsG0880043989.01.T01 AT1G23120 29.333 150 98 5 3 149 1 145 4.90e-16 70.9
MsG0880043989.01.T01 AT1G14950 28.025 157 109 4 1 155 1 155 5.48e-16 70.9
MsG0880043989.01.T01 AT1G35260 29.932 147 92 5 9 149 8 149 1.25e-15 70.1
MsG0880043989.01.T01 AT1G23120 28.369 141 95 4 10 149 60 195 1.41e-15 70.9
MsG0880043989.01.T01 AT1G30990 28.477 151 103 5 1 149 1 148 5.33e-14 65.9
MsG0880043989.01.T01 AT1G14960 27.097 155 109 4 1 153 1 153 2.51e-13 63.9
MsG0880043989.01.T01 AT3G26460 27.451 153 108 3 1 151 1 152 7.59e-13 62.8
MsG0880043989.01.T01 AT1G35310 26.619 139 98 4 13 149 12 148 9.66e-12 59.7
MsG0880043989.01.T01 AT3G26450 25.325 154 110 5 1 151 1 152 1.39e-11 59.3

Find 27 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACATACAAGCTATT+TGG 0.273919 8:-33893434 MsG0880043989.01.T01:CDS
TGGAGATATTGAAAACTATA+AGG 0.391039 8:-33893411 MsG0880043989.01.T01:CDS
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8:-33893352 MsG0880043989.01.T01:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8:-33894984 MsG0880043989.01.T01:CDS
AAACACTGGACTTATGTCAT+AGG 0.470664 8:-33894831 MsG0880043989.01.T01:intron
AGGTAGTCCATCCATCCATT+TGG 0.478602 8:+33893290 None:intergenic
AGCTTCATGAGGGTGAAGAT+TGG 0.493681 8:-33894872 MsG0880043989.01.T01:CDS
TCATCTTGTCAAAGGGGAAA+AGG 0.504175 8:-33893240 MsG0880043989.01.T01:CDS
AGATTGGCATCATACTGATT+CGG 0.504369 8:-33894856 MsG0880043989.01.T01:CDS
AAGAGCACTAGAGATATTGA+TGG 0.505384 8:-33893263 MsG0880043989.01.T01:CDS
TTGATGGTCATCTTGTCAAA+GGG 0.506740 8:-33893247 MsG0880043989.01.T01:CDS
ATCTTCACCCTCATGAAGCT+TGG 0.519812 8:+33894875 None:intergenic
ATCACATACAAGCTATTTGG+TGG 0.530005 8:-33893431 MsG0880043989.01.T01:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8:-33894983 MsG0880043989.01.T01:CDS
ATTGATGGTCATCTTGTCAA+AGG 0.543582 8:-33893248 MsG0880043989.01.T01:CDS
GAGGATGTTGATACTCCAAA+TGG 0.546873 8:-33893305 MsG0880043989.01.T01:CDS
TATCTCTAGTGCTCTTGCTA+AGG 0.569595 8:+33893270 None:intergenic
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8:-33893371 MsG0880043989.01.T01:CDS
TGATACTCCAAATGGATGGA+TGG 0.602025 8:-33893297 MsG0880043989.01.T01:CDS
ATACTGATTCGGTTAAACAC+TGG 0.606046 8:-33894845 MsG0880043989.01.T01:CDS
TCATCATACCAAGCTTCATG+AGG 0.610699 8:-33894883 MsG0880043989.01.T01:CDS
ATGTTGATACTCCAAATGGA+TGG 0.627228 8:-33893301 MsG0880043989.01.T01:CDS
CATCATACCAAGCTTCATGA+GGG 0.642119 8:-33894882 MsG0880043989.01.T01:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8:-33893378 MsG0880043989.01.T01:CDS
TGATGGTCATCTTGTCAAAG+GGG 0.652198 8:-33893246 MsG0880043989.01.T01:CDS
TGACATAACAACAGATGGCG+AGG 0.663434 8:-33893504 MsG0880043989.01.T01:intron
TGATTATGAGAAGAACAATG+AGG 0.694185 8:-33893324 MsG0880043989.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAAAAAAATGTGAAA+GGG - Chr8:33894340-33894359 MsG0880043989.01.T01:intron 10.0%
!!! CTATTATTACATTATTTTTT+TGG + Chr8:33894197-33894216 None:intergenic 10.0%
!! ACTCTTAATCAAAAAAATAT+TGG + Chr8:33893787-33893806 None:intergenic 15.0%
!! CAATTATAAATTAAGTCAAT+AGG + Chr8:33893621-33893640 None:intergenic 15.0%
!! GAATAATATATATTATGAGT+GGG + Chr8:33893833-33893852 None:intergenic 15.0%
!! TATAGTAATAAAAAGAAGAT+TGG - Chr8:33893507-33893526 MsG0880043989.01.T01:intron 15.0%
!! TGAATAATATATATTATGAG+TGG + Chr8:33893834-33893853 None:intergenic 15.0%
!!! CATTTTTAAAAAAATGTGAA+AGG - Chr8:33894339-33894358 MsG0880043989.01.T01:intron 15.0%
!!! GGATTTATATTTTGTTTTTT+AGG - Chr8:33894250-33894269 MsG0880043989.01.T01:intron 15.0%
!!! GTTTTCTATTACAAATTTTT+TGG - Chr8:33893476-33893495 MsG0880043989.01.T01:CDS 15.0%
!!! TTACATTATTTTTTTGGAAT+CGG + Chr8:33894191-33894210 None:intergenic 15.0%
!! CTAGAGATAATTAAAAAGTA+GGG - Chr8:33893988-33894007 MsG0880043989.01.T01:intron 20.0%
!! TCTAGAGATAATTAAAAAGT+AGG - Chr8:33893987-33894006 MsG0880043989.01.T01:intron 20.0%
!! TCTTCGTTATATAATTCATT+TGG + Chr8:33893547-33893566 None:intergenic 20.0%
!! TTTATTTAAAGATCTAAGGA+TGG + Chr8:33894373-33894392 None:intergenic 20.0%
!!! ATTGCCTTTTTTTTTTTTTG+TGG + Chr8:33894549-33894568 None:intergenic 20.0%
!!! CAATAAAATACGTTTTTCAA+AGG + Chr8:33894681-33894700 None:intergenic 20.0%
!!! TATGTATTTGATTTATGCTT+AGG - Chr8:33894040-33894059 MsG0880043989.01.T01:intron 20.0%
!!! TTTTGTTTTTTAGGATACAA+AGG - Chr8:33894259-33894278 MsG0880043989.01.T01:intron 20.0%
! AAAAAATTATACTCGTACCA+AGG + Chr8:33893933-33893952 None:intergenic 25.0%
! AAAATCACATACAAGCTATT+TGG - Chr8:33894791-33894810 MsG0880043989.01.T01:intron 25.0%
! ACCACCACAAAAAAAAAAAA+AGG - Chr8:33894542-33894561 MsG0880043989.01.T01:intron 25.0%
! GTAAACTTATTACTGAACTT+GGG - Chr8:33893242-33893261 MsG0880043989.01.T01:CDS 25.0%
! TGGAGATATTGAAAACTATA+AGG - Chr8:33894814-33894833 MsG0880043989.01.T01:intron 25.0%
!! TCATTTTACAACAGTAATGT+GGG - Chr8:33894229-33894248 MsG0880043989.01.T01:intron 25.0%
!!! ATCTTTAAACAGTGTTTTAG+AGG - Chr8:33894438-33894457 MsG0880043989.01.T01:intron 25.0%
!!! GTGCTTTATTTAAAGATCTA+AGG + Chr8:33894377-33894396 None:intergenic 25.0%
!!! TTTTTTTTTTTTGTGGTGGT+CGG + Chr8:33894542-33894561 None:intergenic 25.0%
AAAGCAACAAATGACCTTAA+AGG - Chr8:33893875-33893894 MsG0880043989.01.T01:intron 30.0%
AAAGTAGGGTTTGCATAATA+TGG - Chr8:33894002-33894021 MsG0880043989.01.T01:intron 30.0%
AAGTAGGGTTTGCATAATAT+GGG - Chr8:33894003-33894022 MsG0880043989.01.T01:intron 30.0%
AGCCATGAAATTACAAATCA+TGG + Chr8:33894116-33894135 None:intergenic 30.0%
AGTAGCATTTCTTTCCTTTA+AGG + Chr8:33893892-33893911 None:intergenic 30.0%
GGTAAACTTATTACTGAACT+TGG - Chr8:33893241-33893260 MsG0880043989.01.T01:CDS 30.0%
TAATTTCATGGCTCTTTCTT+GGG - Chr8:33894123-33894142 MsG0880043989.01.T01:intron 30.0%
TGATTATGAGAAGAACAATG+AGG - Chr8:33894901-33894920 MsG0880043989.01.T01:CDS 30.0%
TTATCTCTAGACAAAAACTC+TGG + Chr8:33893978-33893997 None:intergenic 30.0%
! TGCCATGATTTGTAATTTCA+TGG - Chr8:33894111-33894130 MsG0880043989.01.T01:intron 30.0%
!! GTCATTTTACAACAGTAATG+TGG - Chr8:33894228-33894247 MsG0880043989.01.T01:intron 30.0%
!!! GCCTTTTTTTTTTTTTGTGG+TGG + Chr8:33894546-33894565 None:intergenic 30.0%
!!! TTTTTTTTTTTGTGGTGGTC+GGG + Chr8:33894541-33894560 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG - Chr8:33893394-33893413 MsG0880043989.01.T01:CDS 35.0%
AAATGCTACTCAACCATCTT+TGG - Chr8:33893902-33893921 MsG0880043989.01.T01:intron 35.0%
ATCACATACAAGCTATTTGG+TGG - Chr8:33894794-33894813 MsG0880043989.01.T01:intron 35.0%
ATGGAAATTCTGCTGTTAGA+TGG - Chr8:33894873-33894892 MsG0880043989.01.T01:CDS 35.0%
ATGTTGATACTCCAAATGGA+TGG - Chr8:33894924-33894943 MsG0880043989.01.T01:CDS 35.0%
GTAATTTCATGGCTCTTTCT+TGG - Chr8:33894122-33894141 MsG0880043989.01.T01:intron 35.0%
TGATTTATGCTTAGGAGTTC+AGG - Chr8:33894048-33894067 MsG0880043989.01.T01:intron 35.0%
! AGATTGGCATCATACTGATT+CGG - Chr8:33893369-33893388 MsG0880043989.01.T01:CDS 35.0%
! AGTTTGTAGAACTTTTCAGC+TGG + Chr8:33893277-33893296 None:intergenic 35.0%
! ATTGATGGTCATCTTGTCAA+AGG - Chr8:33894977-33894996 MsG0880043989.01.T01:CDS 35.0%
! TTGATGGTCATCTTGTCAAA+GGG - Chr8:33894978-33894997 MsG0880043989.01.T01:CDS 35.0%
!! AAGAGCACTAGAGATATTGA+TGG - Chr8:33894962-33894981 MsG0880043989.01.T01:CDS 35.0%
!! ATACTGATTCGGTTAAACAC+TGG - Chr8:33893380-33893399 MsG0880043989.01.T01:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - Chr8:33894847-33894866 MsG0880043989.01.T01:CDS 35.0%
AACCATCTTTGGATTCACCT+TGG - Chr8:33893913-33893932 MsG0880043989.01.T01:intron 40.0%
CATCATACCAAGCTTCATGA+GGG - Chr8:33893343-33893362 MsG0880043989.01.T01:CDS 40.0%
GAGGATGTTGATACTCCAAA+TGG - Chr8:33894920-33894939 MsG0880043989.01.T01:CDS 40.0%
GATGTCGAAATCATTGCACT+AGG - Chr8:33893722-33893741 MsG0880043989.01.T01:intron 40.0%
TACCAAGGTGAATCCAAAGA+TGG + Chr8:33893918-33893937 None:intergenic 40.0%
TATCTCTAGTGCTCTTGCTA+AGG + Chr8:33894958-33894977 None:intergenic 40.0%
TCATCATACCAAGCTTCATG+AGG - Chr8:33893342-33893361 MsG0880043989.01.T01:CDS 40.0%
TCATCTTGTCAAAGGGGAAA+AGG - Chr8:33894985-33895004 MsG0880043989.01.T01:CDS 40.0%
TGATACTCCAAATGGATGGA+TGG - Chr8:33894928-33894947 MsG0880043989.01.T01:CDS 40.0%
TGATGGTCATCTTGTCAAAG+GGG - Chr8:33894979-33894998 MsG0880043989.01.T01:CDS 40.0%
AGCTTCATGAGGGTGAAGAT+TGG - Chr8:33893353-33893372 MsG0880043989.01.T01:CDS 45.0%
AGGTAGTCCATCCATCCATT+TGG + Chr8:33894938-33894957 None:intergenic 45.0%
ATCTTCACCCTCATGAAGCT+TGG + Chr8:33893353-33893372 None:intergenic 45.0%
CTTGGGTGCAATGTATGCTT+GGG - Chr8:33894140-33894159 MsG0880043989.01.T01:intron 45.0%
GCCATCTGTTGTTATGTCAG+AGG + Chr8:33894720-33894739 None:intergenic 45.0%
GCCTCTGACATAACAACAGA+TGG - Chr8:33894716-33894735 MsG0880043989.01.T01:intron 45.0%
TCTTGGGTGCAATGTATGCT+TGG - Chr8:33894139-33894158 MsG0880043989.01.T01:intron 45.0%
TGACATAACAACAGATGGCG+AGG - Chr8:33894721-33894740 MsG0880043989.01.T01:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - Chr8:33894854-33894873 MsG0880043989.01.T01:CDS 45.0%
! TGTGCTTAGCTCAGTTGGTA+TGG - Chr8:33894480-33894499 MsG0880043989.01.T01:intron 45.0%
!! AATATATAAGATTATAATAA+AGG + Chr8:33894618-33894637 None:intergenic 5.0%
!!! GTATTATATATTTTTTTAAT+GGG - Chr8:33894580-33894599 MsG0880043989.01.T01:intron 5.0%
!!! TAATTTATAATTGTTTTAAT+TGG - Chr8:33893628-33893647 MsG0880043989.01.T01:intron 5.0%
!!! TATTATATATTTTTTTAATG+GGG - Chr8:33894581-33894600 MsG0880043989.01.T01:intron 5.0%
!!! TGTATTATATATTTTTTTAA+TGG - Chr8:33894579-33894598 MsG0880043989.01.T01:intron 5.0%
!!! TTATATATTATAATATTTTC+TGG - Chr8:33894629-33894648 MsG0880043989.01.T01:intron 5.0%
TGGTGGTCGGGATTTGAACT+CGG + Chr8:33894529-33894548 None:intergenic 50.0%
CCAACTGAGCTAAGCACACG+AGG + Chr8:33894478-33894497 None:intergenic 55.0%
CCTCGTGTGCTTAGCTCAGT+TGG - Chr8:33894475-33894494 MsG0880043989.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33893229 33895018 33893229 ID=MsG0880043989.01;Name=MsG0880043989.01
Chr8 mRNA 33893229 33895018 33893229 ID=MsG0880043989.01.T01;Parent=MsG0880043989.01;Name=MsG0880043989.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|156
Chr8 exon 33894832 33895018 33894832 ID=MsG0880043989.01.T01:exon:10309;Parent=MsG0880043989.01.T01
Chr8 exon 33893229 33893512 33893229 ID=MsG0880043989.01.T01:exon:10308;Parent=MsG0880043989.01.T01
Chr8 CDS 33894832 33895018 33894832 ID=MsG0880043989.01.T01:cds;Parent=MsG0880043989.01.T01
Chr8 CDS 33893229 33893512 33893229 ID=MsG0880043989.01.T01:cds;Parent=MsG0880043989.01.T01
Gene Sequence

>MsG0880043989.01.T01

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCAGCTGAAAAGTTCTACAAACTATTTGCATCAGAACTTCACGAAGTGCAAAATGTTTGTGAAAGAGTTCATCATACCAAGCTTCATGAGGGTGAAGATTGGCATCATACTGATTCGGTTAAACACTGGACTTATGTCATAGATGGCGAGGTACACACATGCAATGAGAGTGTTGAAGAAGTTGATGATCAGAACAAAAAAATCACATACAAGCTATTTGGTGGAGATATTGAAAACTATAAGGTATTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGATGGATGGACTACCTTAGCAAGAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MsG0880043989.01.T01

MVLAGKLITELGIKTPAEKFYKLFASELHEVQNVCERVHHTKLHEGEDWHHTDSVKHWTYVIDGEVHTCNESVEEVDDQNKKITYKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNNEDVDTPNGWMDYLSKSTRDIDGHLVKGEKVAL*