Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011756.01.T01 | XP_003598326.1 | 93.727 | 271 | 17 | 0 | 19 | 289 | 67 | 337 | 6.29E-178 | 510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011756.01.T01 | Q9C9H5 | 78.988 | 257 | 54 | 0 | 18 | 274 | 65 | 321 | 3.38E-144 | 417 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011756.01.T01 | G7IVD9 | 93.727 | 271 | 17 | 0 | 19 | 289 | 67 | 337 | 3.01e-178 | 510 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002808.01 | MsG0380011756.01 | 0.849878 | 2.436265e-60 | 4.685513e-57 |
| MsG0180004688.01 | MsG0380011756.01 | 0.814461 | 1.523032e-51 | 1.022581e-48 |
| MsG0180005621.01 | MsG0380011756.01 | 0.817673 | 2.919213e-52 | 2.139675e-49 |
| MsG0280006316.01 | MsG0380011756.01 | 0.828386 | 9.255880e-55 | 9.186417e-52 |
| MsG0280010617.01 | MsG0380011756.01 | 0.831654 | 1.476460e-55 | 1.612540e-52 |
| MsG0280010825.01 | MsG0380011756.01 | 0.827137 | 1.847504e-54 | 1.767921e-51 |
| MsG0280011016.01 | MsG0380011756.01 | 0.821000 | 5.095025e-53 | 4.095366e-50 |
| MsG0380011756.01 | MsG0380015730.01 | 0.830610 | 2.665448e-55 | 2.822478e-52 |
| MsG0380011756.01 | MsG0380016031.01 | 0.807200 | 5.676893e-50 | 3.142460e-47 |
| MsG0380011756.01 | MsG0380016456.01 | -0.804978 | 1.666571e-49 | 8.713681e-47 |
| MsG0380011756.01 | MsG0380017380.01 | 0.818446 | 1.951785e-52 | 1.461492e-49 |
| MsG0380011756.01 | MsG0380017658.01 | 0.800764 | 1.236754e-48 | 5.806320e-46 |
| MsG0380011756.01 | MsG0480018629.01 | -0.811927 | 5.481255e-51 | 3.436964e-48 |
| MsG0380011756.01 | MsG0480021740.01 | 0.800838 | 1.194474e-48 | 5.618594e-46 |
| MsG0380011756.01 | MsG0480021773.01 | 0.801956 | 7.051018e-49 | 3.411666e-46 |
| MsG0380011756.01 | MsG0480022244.01 | -0.801750 | 7.770952e-49 | 3.740352e-46 |
| MsG0380011756.01 | MsG0480022378.01 | 0.817945 | 2.534222e-52 | 1.871323e-49 |
| MsG0380011756.01 | MsG0480023532.01 | 0.809278 | 2.048209e-50 | 1.197451e-47 |
| MsG0380011756.01 | MsG0480023913.01 | 0.816646 | 4.967621e-52 | 3.540053e-49 |
| MsG0380011756.01 | MsG0580025007.01 | 0.808265 | 3.372056e-50 | 1.919411e-47 |
| MsG0380011756.01 | MsG0580025023.01 | 0.813276 | 2.778449e-51 | 1.806431e-48 |
| MsG0380011756.01 | MsG0580026449.01 | 0.800772 | 1.231769e-48 | 5.784105e-46 |
| MsG0380011756.01 | MsG0580028510.01 | 0.824842 | 6.482996e-54 | 5.809868e-51 |
| MsG0380011756.01 | MsG0680031356.01 | 0.840722 | 7.325455e-58 | 1.052715e-54 |
| MsG0380011756.01 | MsG0780035917.01 | 0.816150 | 6.413755e-52 | 4.509148e-49 |
| MsG0380011756.01 | MsG0780040459.01 | 0.801063 | 1.074573e-48 | 5.083673e-46 |
| MsG0380011756.01 | MsG0780041100.01 | 0.846225 | 2.481863e-59 | 4.242245e-56 |
| MsG0380011756.01 | MsG0780041820.01 | 0.838914 | 2.166280e-57 | 2.943780e-54 |
| MsG0380011756.01 | MsG0880044688.01 | 0.808063 | 3.722787e-50 | 2.107672e-47 |
| MsG0380011756.01 | MsG0880045802.01 | 0.840070 | 1.084946e-57 | 1.528063e-54 |
| MsG0380011756.01 | MsG0880047450.01 | 0.834149 | 3.542155e-56 | 4.166696e-53 |
| MsG0280006851.01 | MsG0380011756.01 | 0.813253 | 2.810161e-51 | 1.825844e-48 |
| MsG0280006909.01 | MsG0380011756.01 | 0.818311 | 2.094419e-52 | 1.562266e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011756.01.T01 | MTR_3g010330 | 93.727 | 271 | 17 | 0 | 19 | 289 | 67 | 337 | 0.0 | 510 |
| MsG0380011756.01.T01 | MTR_3g080810 | 59.770 | 261 | 101 | 1 | 18 | 274 | 76 | 336 | 2.90e-103 | 311 |
| MsG0380011756.01.T01 | MTR_8g104820 | 52.536 | 276 | 126 | 3 | 19 | 289 | 72 | 347 | 3.18e-92 | 282 |
| MsG0380011756.01.T01 | MTR_3g117500 | 54.962 | 262 | 110 | 4 | 20 | 274 | 67 | 327 | 1.57e-91 | 280 |
| MsG0380011756.01.T01 | MTR_4g035955 | 53.876 | 258 | 117 | 2 | 17 | 274 | 54 | 309 | 1.97e-90 | 277 |
| MsG0380011756.01.T01 | MTR_1g088190 | 57.255 | 255 | 104 | 2 | 25 | 275 | 69 | 322 | 1.26e-88 | 268 |
| MsG0380011756.01.T01 | MTR_1g088190 | 55.390 | 269 | 115 | 2 | 25 | 289 | 69 | 336 | 2.27e-87 | 270 |
| MsG0380011756.01.T01 | MTR_4g035975 | 50.345 | 290 | 138 | 4 | 18 | 304 | 75 | 361 | 3.67e-86 | 267 |
| MsG0380011756.01.T01 | MTR_4g035930 | 51.550 | 258 | 123 | 2 | 17 | 274 | 55 | 310 | 5.69e-86 | 266 |
| MsG0380011756.01.T01 | MTR_3g105230 | 41.549 | 284 | 157 | 4 | 19 | 300 | 72 | 348 | 4.62e-71 | 228 |
| MsG0380011756.01.T01 | MTR_5g009970 | 46.565 | 262 | 130 | 5 | 16 | 274 | 6 | 260 | 2.34e-70 | 224 |
| MsG0380011756.01.T01 | MTR_5g010000 | 49.035 | 259 | 122 | 5 | 19 | 274 | 82 | 333 | 8.88e-70 | 225 |
| MsG0380011756.01.T01 | MTR_5g085730 | 42.617 | 298 | 149 | 8 | 19 | 300 | 73 | 364 | 5.80e-69 | 223 |
| MsG0380011756.01.T01 | MTR_5g009970 | 46.718 | 259 | 128 | 5 | 19 | 274 | 83 | 334 | 6.93e-69 | 223 |
| MsG0380011756.01.T01 | MTR_8g099410 | 49.020 | 255 | 115 | 7 | 19 | 261 | 70 | 321 | 9.23e-68 | 220 |
| MsG0380011756.01.T01 | MTR_3g080190 | 43.537 | 294 | 152 | 7 | 19 | 300 | 82 | 373 | 1.04e-67 | 220 |
| MsG0380011756.01.T01 | MTR_3g023740 | 43.662 | 284 | 151 | 4 | 19 | 300 | 72 | 348 | 3.06e-67 | 218 |
| MsG0380011756.01.T01 | MTR_8g099410 | 49.020 | 255 | 115 | 7 | 19 | 261 | 70 | 321 | 4.70e-67 | 221 |
| MsG0380011756.01.T01 | MTR_5g093590 | 43.051 | 295 | 153 | 6 | 19 | 300 | 76 | 368 | 1.24e-63 | 209 |
| MsG0380011756.01.T01 | MTR_1g037340 | 48.780 | 246 | 117 | 5 | 19 | 258 | 67 | 309 | 1.60e-61 | 202 |
| MsG0380011756.01.T01 | MTR_3g113720 | 43.657 | 268 | 134 | 6 | 19 | 272 | 98 | 362 | 4.46e-59 | 197 |
| MsG0380011756.01.T01 | MTR_5g009970 | 44.167 | 240 | 124 | 5 | 38 | 274 | 1 | 233 | 2.96e-57 | 189 |
| MsG0380011756.01.T01 | MTR_4g074960 | 46.642 | 268 | 127 | 7 | 19 | 274 | 71 | 334 | 8.60e-57 | 193 |
| MsG0380011756.01.T01 | MTR_8g075990 | 44.238 | 269 | 133 | 7 | 19 | 273 | 104 | 369 | 5.60e-53 | 181 |
| MsG0380011756.01.T01 | MTR_3g082440 | 42.213 | 244 | 112 | 11 | 21 | 241 | 162 | 399 | 8.35e-38 | 142 |
| MsG0380011756.01.T01 | MTR_2g028480 | 44.492 | 236 | 101 | 10 | 20 | 232 | 159 | 387 | 2.79e-36 | 137 |
| MsG0380011756.01.T01 | MTR_3g090540 | 37.449 | 243 | 122 | 10 | 18 | 235 | 148 | 385 | 2.07e-35 | 135 |
| MsG0380011756.01.T01 | MTR_5g009970 | 43.860 | 171 | 89 | 3 | 105 | 274 | 1 | 165 | 3.33e-34 | 127 |
| MsG0380011756.01.T01 | MTR_1g037320 | 48.466 | 163 | 79 | 3 | 38 | 198 | 1 | 160 | 6.79e-32 | 120 |
| MsG0380011756.01.T01 | MTR_7g108920 | 50.847 | 59 | 25 | 1 | 186 | 240 | 86 | 144 | 3.55e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011756.01.T01 | AT1G22880 | 77.273 | 264 | 60 | 0 | 18 | 281 | 65 | 328 | 3.80e-148 | 419 |
| MsG0380011756.01.T01 | AT1G71380 | 78.988 | 257 | 54 | 0 | 18 | 274 | 65 | 321 | 3.44e-145 | 417 |
| MsG0380011756.01.T01 | AT1G22880 | 78.210 | 257 | 56 | 0 | 18 | 274 | 65 | 321 | 1.02e-144 | 416 |
| MsG0380011756.01.T01 | AT1G22880 | 76.793 | 237 | 55 | 0 | 38 | 274 | 1 | 237 | 6.60e-130 | 375 |
| MsG0380011756.01.T01 | AT4G02290 | 58.845 | 277 | 109 | 2 | 17 | 289 | 92 | 367 | 8.59e-103 | 310 |
| MsG0380011756.01.T01 | AT1G02800 | 57.092 | 282 | 114 | 3 | 17 | 294 | 83 | 361 | 4.20e-100 | 303 |
| MsG0380011756.01.T01 | AT1G70710 | 54.909 | 275 | 119 | 2 | 19 | 289 | 70 | 343 | 5.01e-99 | 300 |
| MsG0380011756.01.T01 | AT4G39010 | 52.874 | 261 | 116 | 3 | 19 | 272 | 71 | 331 | 1.71e-96 | 290 |
| MsG0380011756.01.T01 | AT1G23210 | 54.182 | 275 | 121 | 2 | 19 | 289 | 70 | 343 | 8.16e-96 | 291 |
| MsG0380011756.01.T01 | AT4G39010 | 52.874 | 261 | 116 | 3 | 19 | 272 | 71 | 331 | 6.84e-95 | 290 |
| MsG0380011756.01.T01 | AT4G02290 | 57.031 | 256 | 105 | 2 | 38 | 289 | 1 | 255 | 3.96e-89 | 272 |
| MsG0380011756.01.T01 | AT3G43860 | 47.735 | 287 | 141 | 4 | 16 | 300 | 73 | 352 | 2.75e-78 | 246 |
| MsG0380011756.01.T01 | AT3G43860 | 47.735 | 287 | 141 | 4 | 16 | 300 | 73 | 352 | 2.75e-78 | 246 |
| MsG0380011756.01.T01 | AT4G38990 | 47.547 | 265 | 132 | 3 | 17 | 274 | 63 | 327 | 1.91e-75 | 239 |
| MsG0380011756.01.T01 | AT4G39000 | 47.547 | 265 | 132 | 3 | 17 | 274 | 65 | 329 | 2.88e-73 | 234 |
| MsG0380011756.01.T01 | AT2G32990 | 44.932 | 296 | 145 | 8 | 19 | 300 | 81 | 372 | 2.44e-70 | 227 |
| MsG0380011756.01.T01 | AT4G23560 | 50.193 | 259 | 119 | 5 | 19 | 274 | 65 | 316 | 9.30e-68 | 219 |
| MsG0380011756.01.T01 | AT4G11050 | 44.898 | 294 | 146 | 9 | 19 | 300 | 68 | 357 | 1.24e-66 | 218 |
| MsG0380011756.01.T01 | AT1G75680 | 45.734 | 293 | 144 | 8 | 19 | 300 | 98 | 386 | 3.31e-66 | 216 |
| MsG0380011756.01.T01 | AT4G09740 | 47.059 | 289 | 127 | 8 | 19 | 289 | 65 | 345 | 3.64e-66 | 215 |
| MsG0380011756.01.T01 | AT4G11050 | 44.898 | 294 | 146 | 9 | 19 | 300 | 68 | 357 | 4.22e-66 | 218 |
| MsG0380011756.01.T01 | AT4G11050 | 44.898 | 294 | 146 | 9 | 19 | 300 | 68 | 357 | 4.53e-66 | 218 |
| MsG0380011756.01.T01 | AT1G64390 | 43.537 | 294 | 150 | 8 | 19 | 300 | 67 | 356 | 4.19e-64 | 213 |
| MsG0380011756.01.T01 | AT1G19940 | 43.609 | 266 | 137 | 6 | 19 | 274 | 89 | 351 | 2.40e-63 | 206 |
| MsG0380011756.01.T01 | AT4G38990 | 43.346 | 263 | 121 | 3 | 17 | 274 | 63 | 302 | 7.67e-63 | 202 |
| MsG0380011756.01.T01 | AT1G19940 | 43.609 | 266 | 137 | 6 | 19 | 274 | 89 | 351 | 3.37e-62 | 205 |
| MsG0380011756.01.T01 | AT1G19940 | 43.609 | 266 | 137 | 6 | 19 | 274 | 89 | 351 | 3.37e-62 | 205 |
| MsG0380011756.01.T01 | AT2G44540 | 47.368 | 247 | 121 | 6 | 18 | 258 | 74 | 317 | 8.01e-60 | 199 |
| MsG0380011756.01.T01 | AT2G44560 | 46.964 | 247 | 122 | 6 | 18 | 258 | 74 | 317 | 1.65e-57 | 192 |
| MsG0380011756.01.T01 | AT2G44550 | 46.341 | 246 | 124 | 5 | 18 | 258 | 74 | 316 | 2.22e-57 | 192 |
| MsG0380011756.01.T01 | AT2G44570 | 46.154 | 247 | 124 | 6 | 18 | 258 | 74 | 317 | 5.62e-57 | 189 |
| MsG0380011756.01.T01 | AT2G44570 | 46.154 | 247 | 124 | 6 | 18 | 258 | 74 | 317 | 3.34e-56 | 189 |
| MsG0380011756.01.T01 | AT1G48930 | 46.269 | 268 | 128 | 7 | 19 | 274 | 72 | 335 | 1.24e-53 | 185 |
| MsG0380011756.01.T01 | AT4G24260 | 41.111 | 270 | 127 | 10 | 20 | 262 | 156 | 420 | 2.72e-42 | 152 |
| MsG0380011756.01.T01 | AT4G24260 | 41.111 | 270 | 127 | 10 | 20 | 262 | 156 | 420 | 8.77e-42 | 150 |
| MsG0380011756.01.T01 | AT4G24260 | 41.111 | 270 | 127 | 10 | 20 | 262 | 156 | 420 | 5.20e-41 | 150 |
| MsG0380011756.01.T01 | AT5G49720 | 45.957 | 235 | 98 | 9 | 20 | 231 | 155 | 383 | 1.23e-38 | 144 |
| MsG0380011756.01.T01 | AT1G65610 | 37.313 | 268 | 135 | 12 | 21 | 261 | 161 | 422 | 2.03e-31 | 124 |
Find 57 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAATCATTCTCTATTCT+TGG | 0.199636 | 3:+4568369 | None:intergenic |
| TGCTGTTAAATACTACAATT+TGG | 0.250375 | 3:-4568768 | MsG0380011756.01.T01:CDS |
| CTACAATTTGGCCAAGTCTT+TGG | 0.251872 | 3:-4568756 | MsG0380011756.01.T01:CDS |
| CCAGAATATGAGCAATAAAA+TGG | 0.291892 | 3:+4569181 | None:intergenic |
| TACAATTTGGCCAAGTCTTT+GGG | 0.295167 | 3:-4568755 | MsG0380011756.01.T01:CDS |
| CAACATTTGTGATCAACATT+TGG | 0.314931 | 3:+4569436 | None:intergenic |
| TTATCCCAGCTGAAGATATC+AGG | 0.329473 | 3:+4568719 | None:intergenic |
| TCATGGAGCACAATTGAATA+TGG | 0.352249 | 3:-4569574 | MsG0380011756.01.T01:CDS |
| GCTCTTGCTTCTTTCATTTG+TGG | 0.396038 | 3:+4569538 | None:intergenic |
| ACTATAAATATAATAGTTGA+TGG | 0.397288 | 3:+4568405 | None:intergenic |
| TTCAGCAGCAACATCAGAAC+CGG | 0.401931 | 3:+4569354 | None:intergenic |
| ACACCGACATAGAGTCTTCC+AGG | 0.415923 | 3:+4569466 | None:intergenic |
| CAGTTTGCTTTGCAGTATCA+AGG | 0.418073 | 3:-4569238 | MsG0380011756.01.T01:CDS |
| TTGCTCATATTCTGGATTCA+AGG | 0.420522 | 3:-4569173 | MsG0380011756.01.T01:intron |
| CTAACAGTGTCCATATCTTC+TGG | 0.423308 | 3:+4569406 | None:intergenic |
| TTGGGAAAGACCAGAAGATA+TGG | 0.432588 | 3:-4569416 | MsG0380011756.01.T01:CDS |
| CAAATGTTGATCACAAATGT+TGG | 0.436805 | 3:-4569435 | MsG0380011756.01.T01:CDS |
| GTGTTAGCACTTTGTTCCAT+TGG | 0.437623 | 3:+4569700 | None:intergenic |
| TGTAGTTGAAGAGGGTGAAT+TGG | 0.441166 | 3:+4568486 | None:intergenic |
| AATTGAATATGGAAAGAGAA+TGG | 0.462216 | 3:-4569563 | MsG0380011756.01.T01:CDS |
| ATCAACCTGATATCTTCAGC+TGG | 0.462559 | 3:-4568724 | MsG0380011756.01.T01:CDS |
| AACAAAAGCTTAGAATAAGT+TGG | 0.464270 | 3:+4569283 | None:intergenic |
| AAATGTTGATCACAAATGTT+GGG | 0.467634 | 3:-4569434 | MsG0380011756.01.T01:CDS |
| TCTTCAGGATGAACTATTGT+GGG | 0.468517 | 3:-4568819 | MsG0380011756.01.T01:intron |
| GTGCACATGTCCTTCTCTCA+AGG | 0.474161 | 3:-4568685 | MsG0380011756.01.T01:CDS |
| ATTGAATATGGAAAGAGAAT+GGG | 0.486477 | 3:-4569562 | MsG0380011756.01.T01:CDS |
| ATTGTTGTTGTGAATGCCAT+TGG | 0.494336 | 3:+4569601 | None:intergenic |
| ACAGGAGGCTATTATGATGC+AGG | 0.500795 | 3:-4569646 | MsG0380011756.01.T01:CDS |
| ATCTGTGGCGTGGCGAATTG+CGG | 0.500987 | 3:+4569516 | None:intergenic |
| TCACAACAACAATGTTATCA+TGG | 0.509771 | 3:-4569591 | MsG0380011756.01.T01:CDS |
| ATCTTCAGGATGAACTATTG+TGG | 0.519125 | 3:-4568820 | MsG0380011756.01.T01:intron |
| ATTTCACTTGCATTATCCAA+TGG | 0.522169 | 3:-4569716 | None:intergenic |
| ATCATCAGCTCCCAAAGACT+TGG | 0.531480 | 3:+4568745 | None:intergenic |
| GTAGTTGAAGAGGGTGAATT+GGG | 0.534837 | 3:+4568487 | None:intergenic |
| AACTGCTGCTGCACTTGCTG+CGG | 0.539280 | 3:-4569332 | MsG0380011756.01.T01:CDS |
| ATAAGAACTTTGATCAGTAT+AGG | 0.541520 | 3:-4568543 | MsG0380011756.01.T01:CDS |
| TATGTATCTTCAAACAATCC+CGG | 0.544424 | 3:-4569373 | MsG0380011756.01.T01:CDS |
| CTACAGAGAGCATTGCTGAA+TGG | 0.545991 | 3:-4568568 | MsG0380011756.01.T01:intron |
| AGCTGGGATAACAAATATGC+TGG | 0.549315 | 3:-4568707 | MsG0380011756.01.T01:CDS |
| GGAAGACTCTATGTCGGTGT+TGG | 0.560843 | 3:-4569463 | MsG0380011756.01.T01:CDS |
| TTCAACTTACCCTTGAGAGA+AGG | 0.570746 | 3:+4568675 | None:intergenic |
| TTGCAGGTGGATTTGACAGG+AGG | 0.571495 | 3:-4569661 | MsG0380011756.01.T01:CDS |
| TTTGTTGAGGACTTCACAGA+AGG | 0.583258 | 3:-4569266 | MsG0380011756.01.T01:CDS |
| AACTATTGTGGGGAGCTGCA+TGG | 0.591973 | 3:-4568808 | MsG0380011756.01.T01:CDS |
| TGCACATGTCCTTCTCTCAA+GGG | 0.598418 | 3:-4568684 | MsG0380011756.01.T01:intron |
| GGCTCATACAGTGATTCACT+TGG | 0.605518 | 3:-4569217 | MsG0380011756.01.T01:CDS |
| TCAACCTGATATCTTCAGCT+GGG | 0.606032 | 3:-4568723 | MsG0380011756.01.T01:CDS |
| TGTATACTGTGTAGTTGAAG+AGG | 0.614209 | 3:+4568477 | None:intergenic |
| GTGTTGATGTTGCACACTTG+AGG | 0.620658 | 3:+4569488 | None:intergenic |
| AAGTGTGCAACATCAACACC+TGG | 0.627700 | 3:-4569484 | MsG0380011756.01.T01:CDS |
| TCAGCAGCAACATCAGAACC+GGG | 0.629928 | 3:+4569355 | None:intergenic |
| CTTCAGGATGAACTATTGTG+GGG | 0.648937 | 3:-4568818 | MsG0380011756.01.T01:intron |
| GTATACTGTGTAGTTGAAGA+GGG | 0.651354 | 3:+4568478 | None:intergenic |
| TGAGGAGATAATCTGTGGCG+TGG | 0.651976 | 3:+4569506 | None:intergenic |
| ACACCTGGAAGACTCTATGT+CGG | 0.686227 | 3:-4569469 | MsG0380011756.01.T01:CDS |
| ACACTTGAGGAGATAATCTG+TGG | 0.686648 | 3:+4569501 | None:intergenic |
| TCAACTACACAGTATACACA+AGG | 0.701067 | 3:-4568472 | MsG0380011756.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTATAAATATAATAGTTGA+TGG | + | Chr3:4569682-4569701 | None:intergenic | 15.0% |
| !!! | ATAAAATCAATTTTAAACAG+AGG | - | Chr3:4569135-4569154 | MsG0380011756.01.T01:intron | 15.0% |
| !!! | TAAAATCAATTTTAAACAGA+GGG | - | Chr3:4569136-4569155 | MsG0380011756.01.T01:intron | 15.0% |
| !!! | TATAGTTAAATATCTAGTTT+TGG | - | Chr3:4569000-4569019 | MsG0380011756.01.T01:intron | 15.0% |
| !! | AAAGTTTAGACAAAACAAAT+TGG | + | Chr3:4569457-4569476 | None:intergenic | 20.0% |
| ! | AAATGTTGATCACAAATGTT+GGG | - | Chr3:4568650-4568669 | MsG0380011756.01.T01:intron | 25.0% |
| ! | AACAAAAGCTTAGAATAAGT+TGG | + | Chr3:4568804-4568823 | None:intergenic | 25.0% |
| ! | AATAGGCATTATTTATCTTC+AGG | - | Chr3:4569250-4569269 | MsG0380011756.01.T01:CDS | 25.0% |
| ! | AATTGAATATGGAAAGAGAA+TGG | - | Chr3:4568521-4568540 | MsG0380011756.01.T01:CDS | 25.0% |
| ! | ATAAGAACTTTGATCAGTAT+AGG | - | Chr3:4569541-4569560 | MsG0380011756.01.T01:CDS | 25.0% |
| ! | ATTGAATATGGAAAGAGAAT+GGG | - | Chr3:4568522-4568541 | MsG0380011756.01.T01:CDS | 25.0% |
| ! | TCTCTCTAATAGTAAGAAAT+AGG | + | Chr3:4569202-4569221 | None:intergenic | 25.0% |
| ! | TGCTGTTAAATACTACAATT+TGG | - | Chr3:4569316-4569335 | MsG0380011756.01.T01:CDS | 25.0% |
| !! | TGTGAAATTCAATTTTCCAA+TGG | - | Chr3:4568467-4568486 | MsG0380011756.01.T01:CDS | 25.0% |
| !!! | TTTTGGTAGTATCACTATTA+GGG | - | Chr3:4569017-4569036 | MsG0380011756.01.T01:intron | 25.0% |
| CAAATGTTGATCACAAATGT+TGG | - | Chr3:4568649-4568668 | MsG0380011756.01.T01:intron | 30.0% | |
| CAACATTTGTGATCAACATT+TGG | + | Chr3:4568651-4568670 | None:intergenic | 30.0% | |
| CCAGAATATGAGCAATAAAA+TGG | + | Chr3:4568906-4568925 | None:intergenic | 30.0% | |
| TATGTATCTTCAAACAATCC+CGG | - | Chr3:4568711-4568730 | MsG0380011756.01.T01:CDS | 30.0% | |
| TCACAACAACAATGTTATCA+TGG | - | Chr3:4568493-4568512 | MsG0380011756.01.T01:CDS | 30.0% | |
| TCTACTTGTATCGTTCTAAT+AGG | - | Chr3:4569233-4569252 | MsG0380011756.01.T01:CDS | 30.0% | |
| ! | CCATTTTATTGCTCATATTC+TGG | - | Chr3:4568903-4568922 | MsG0380011756.01.T01:intron | 30.0% |
| ! | CTTATTCTAAGCTTTTGTTG+AGG | - | Chr3:4568805-4568824 | MsG0380011756.01.T01:CDS | 30.0% |
| ! | TTGGTTGTTAAGTTTGAGAT+TGG | - | Chr3:4569092-4569111 | MsG0380011756.01.T01:intron | 30.0% |
| ! | TTTGGTAGTATCACTATTAG+GGG | - | Chr3:4569018-4569037 | MsG0380011756.01.T01:intron | 30.0% |
| !! | ATACACAAACATTGTTTTGC+AGG | - | Chr3:4568407-4568426 | MsG0380011756.01.T01:CDS | 30.0% |
| !! | GTTTTGGTAGTATCACTATT+AGG | - | Chr3:4569016-4569035 | MsG0380011756.01.T01:intron | 30.0% |
| ATCTTCAGGATGAACTATTG+TGG | - | Chr3:4569264-4569283 | MsG0380011756.01.T01:CDS | 35.0% | |
| GTATACTGTGTAGTTGAAGA+GGG | + | Chr3:4569609-4569628 | None:intergenic | 35.0% | |
| TACAATTTGGCCAAGTCTTT+GGG | - | Chr3:4569329-4569348 | MsG0380011756.01.T01:CDS | 35.0% | |
| TCAACTACACAGTATACACA+AGG | - | Chr3:4569612-4569631 | MsG0380011756.01.T01:CDS | 35.0% | |
| TCATGGAGCACAATTGAATA+TGG | - | Chr3:4568510-4568529 | MsG0380011756.01.T01:CDS | 35.0% | |
| TCTTCAGGATGAACTATTGT+GGG | - | Chr3:4569265-4569284 | MsG0380011756.01.T01:CDS | 35.0% | |
| TGTATACTGTGTAGTTGAAG+AGG | + | Chr3:4569610-4569629 | None:intergenic | 35.0% | |
| TTGCTCATATTCTGGATTCA+AGG | - | Chr3:4568911-4568930 | MsG0380011756.01.T01:intron | 35.0% | |
| ! | ATTGTTGTTGTGAATGCCAT+TGG | + | Chr3:4568486-4568505 | None:intergenic | 35.0% |
| ! | CTATGTGATACAGTTTTTCG+AGG | - | Chr3:4569173-4569192 | MsG0380011756.01.T01:intron | 35.0% |
| ! | TTACACGTAGCAGACAATTT+TGG | - | Chr3:4569073-4569092 | MsG0380011756.01.T01:intron | 35.0% |
| ACACTTGAGGAGATAATCTG+TGG | + | Chr3:4568586-4568605 | None:intergenic | 40.0% | |
| AGCTGGGATAACAAATATGC+TGG | - | Chr3:4569377-4569396 | MsG0380011756.01.T01:CDS | 40.0% | |
| ATCAACCTGATATCTTCAGC+TGG | - | Chr3:4569360-4569379 | MsG0380011756.01.T01:CDS | 40.0% | |
| CCAATACTCGGATTCTTACT+CGG | - | Chr3:4568973-4568992 | MsG0380011756.01.T01:intron | 40.0% | |
| CTAACAGTGTCCATATCTTC+TGG | + | Chr3:4568681-4568700 | None:intergenic | 40.0% | |
| CTACAATTTGGCCAAGTCTT+TGG | - | Chr3:4569328-4569347 | MsG0380011756.01.T01:CDS | 40.0% | |
| CTTCAGGATGAACTATTGTG+GGG | - | Chr3:4569266-4569285 | MsG0380011756.01.T01:CDS | 40.0% | |
| GCTCTTGCTTCTTTCATTTG+TGG | + | Chr3:4568549-4568568 | None:intergenic | 40.0% | |
| GTAGTTGAAGAGGGTGAATT+GGG | + | Chr3:4569600-4569619 | None:intergenic | 40.0% | |
| GTGTTAGCACTTTGTTCCAT+TGG | + | Chr3:4568387-4568406 | None:intergenic | 40.0% | |
| TAGTATCACTATTAGGGGTC+TGG | - | Chr3:4569023-4569042 | MsG0380011756.01.T01:intron | 40.0% | |
| TCAACCTGATATCTTCAGCT+GGG | - | Chr3:4569361-4569380 | MsG0380011756.01.T01:CDS | 40.0% | |
| TGTAGTTGAAGAGGGTGAAT+TGG | + | Chr3:4569601-4569620 | None:intergenic | 40.0% | |
| TTATCCCAGCTGAAGATATC+AGG | + | Chr3:4569368-4569387 | None:intergenic | 40.0% | |
| TTCAACTTACCCTTGAGAGA+AGG | + | Chr3:4569412-4569431 | None:intergenic | 40.0% | |
| TTGGGAAAGACCAGAAGATA+TGG | - | Chr3:4568668-4568687 | MsG0380011756.01.T01:intron | 40.0% | |
| TTTGTTGAGGACTTCACAGA+AGG | - | Chr3:4568818-4568837 | MsG0380011756.01.T01:intron | 40.0% | |
| ! | CAGTTTGCTTTGCAGTATCA+AGG | - | Chr3:4568846-4568865 | MsG0380011756.01.T01:intron | 40.0% |
| !! | CACAAACATTGTTTTGCAGG+TGG | - | Chr3:4568410-4568429 | MsG0380011756.01.T01:CDS | 40.0% |
| AAGTGTGCAACATCAACACC+TGG | - | Chr3:4568600-4568619 | MsG0380011756.01.T01:intron | 45.0% | |
| ACACCTGGAAGACTCTATGT+CGG | - | Chr3:4568615-4568634 | MsG0380011756.01.T01:intron | 45.0% | |
| ACAGGAGGCTATTATGATGC+AGG | - | Chr3:4568438-4568457 | MsG0380011756.01.T01:CDS | 45.0% | |
| ATCATCAGCTCCCAAAGACT+TGG | + | Chr3:4569342-4569361 | None:intergenic | 45.0% | |
| CCGAGTAAGAATCCGAGTAT+TGG | + | Chr3:4568976-4568995 | None:intergenic | 45.0% | |
| GGCTCATACAGTGATTCACT+TGG | - | Chr3:4568867-4568886 | MsG0380011756.01.T01:intron | 45.0% | |
| GGGTCTGGATTCTCTAATGT+AGG | - | Chr3:4569038-4569057 | MsG0380011756.01.T01:intron | 45.0% | |
| TGCACATGTCCTTCTCTCAA+GGG | - | Chr3:4569400-4569419 | MsG0380011756.01.T01:CDS | 45.0% | |
| TTCAGCAGCAACATCAGAAC+CGG | + | Chr3:4568733-4568752 | None:intergenic | 45.0% | |
| ! | CCTAGAGCATCTCCAATACT+CGG | - | Chr3:4568961-4568980 | MsG0380011756.01.T01:intron | 45.0% |
| ! | GTGTTGATGTTGCACACTTG+AGG | + | Chr3:4568599-4568618 | None:intergenic | 45.0% |
| !! | CTACAGAGAGCATTGCTGAA+TGG | - | Chr3:4569516-4569535 | MsG0380011756.01.T01:CDS | 45.0% |
| !! | GTTTTGCAGGTGGATTTGAC+AGG | - | Chr3:4568420-4568439 | MsG0380011756.01.T01:CDS | 45.0% |
| AACTATTGTGGGGAGCTGCA+TGG | - | Chr3:4569276-4569295 | MsG0380011756.01.T01:CDS | 50.0% | |
| ACACCGACATAGAGTCTTCC+AGG | + | Chr3:4568621-4568640 | None:intergenic | 50.0% | |
| GGAAGACTCTATGTCGGTGT+TGG | - | Chr3:4568621-4568640 | MsG0380011756.01.T01:intron | 50.0% | |
| GTGCACATGTCCTTCTCTCA+AGG | - | Chr3:4569399-4569418 | MsG0380011756.01.T01:CDS | 50.0% | |
| TCAGCAGCAACATCAGAACC+GGG | + | Chr3:4568732-4568751 | None:intergenic | 50.0% | |
| TGAGGAGATAATCTGTGGCG+TGG | + | Chr3:4568581-4568600 | None:intergenic | 50.0% | |
| ! | GGGAGCTGCATGGCTTTTTA+GGG | - | Chr3:4569286-4569305 | MsG0380011756.01.T01:CDS | 50.0% |
| ! | TTGCAGGTGGATTTGACAGG+AGG | - | Chr3:4568423-4568442 | MsG0380011756.01.T01:CDS | 50.0% |
| !! | CCGAGTATTGGAGATGCTCT+AGG | + | Chr3:4568964-4568983 | None:intergenic | 50.0% |
| ATCTGTGGCGTGGCGAATTG+CGG | + | Chr3:4568571-4568590 | None:intergenic | 55.0% | |
| ! | AACTGCTGCTGCACTTGCTG+CGG | - | Chr3:4568752-4568771 | MsG0380011756.01.T01:CDS | 55.0% |
| ! | GGGGAGCTGCATGGCTTTTT+AGG | - | Chr3:4569285-4569304 | MsG0380011756.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 4568375 | 4569731 | 4568375 | ID=MsG0380011756.01;Name=MsG0380011756.01 |
| Chr3 | mRNA | 4568375 | 4569731 | 4568375 | ID=MsG0380011756.01.T01;Parent=MsG0380011756.01;Name=MsG0380011756.01.T01;_AED=0.44;_eAED=0.45;_QI=0|1|0.33|1|1|1|3|0|305 |
| Chr3 | exon | 4569174 | 4569731 | 4569174 | ID=MsG0380011756.01.T01:exon:9562;Parent=MsG0380011756.01.T01 |
| Chr3 | exon | 4568685 | 4568834 | 4568685 | ID=MsG0380011756.01.T01:exon:9561;Parent=MsG0380011756.01.T01 |
| Chr3 | exon | 4568375 | 4568584 | 4568375 | ID=MsG0380011756.01.T01:exon:9560;Parent=MsG0380011756.01.T01 |
| Chr3 | CDS | 4569174 | 4569731 | 4569174 | ID=MsG0380011756.01.T01:cds;Parent=MsG0380011756.01.T01 |
| Chr3 | CDS | 4568685 | 4568834 | 4568685 | ID=MsG0380011756.01.T01:cds;Parent=MsG0380011756.01.T01 |
| Chr3 | CDS | 4568375 | 4568584 | 4568375 | ID=MsG0380011756.01.T01:cds;Parent=MsG0380011756.01.T01 |
| Gene Sequence |
| Protein sequence |
