Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014928.01.T01 | XP_013460370.1 | 94.017 | 468 | 28 | 0 | 1 | 468 | 1 | 468 | 0 | 923 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014928.01.T01 | Q8GYW8 | 38.889 | 468 | 254 | 13 | 8 | 465 | 11 | 456 | 3.66E-92 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014928.01.T01 | A0A072UZ79 | 94.017 | 468 | 28 | 0 | 1 | 468 | 1 | 468 | 0.0 | 923 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014928.01.T01 | MTR_3g465930 | 94.017 | 468 | 28 | 0 | 1 | 468 | 1 | 468 | 0.0 | 923 |
| MsG0380014928.01.T01 | MTR_3g465910 | 88.248 | 468 | 50 | 3 | 1 | 466 | 1 | 465 | 0.0 | 862 |
| MsG0380014928.01.T01 | MTR_3g465950 | 76.116 | 448 | 95 | 3 | 1 | 447 | 1 | 437 | 0.0 | 695 |
| MsG0380014928.01.T01 | MTR_3g465980 | 68.455 | 466 | 139 | 5 | 1 | 464 | 1 | 460 | 0.0 | 664 |
| MsG0380014928.01.T01 | MTR_3g465990 | 66.524 | 466 | 148 | 5 | 1 | 464 | 1 | 460 | 0.0 | 650 |
| MsG0380014928.01.T01 | MTR_3g465880 | 67.161 | 472 | 142 | 8 | 1 | 464 | 1 | 467 | 0.0 | 640 |
| MsG0380014928.01.T01 | MTR_3g465870 | 69.214 | 471 | 100 | 6 | 1 | 468 | 1 | 429 | 0.0 | 639 |
| MsG0380014928.01.T01 | MTR_6g053810 | 60.814 | 467 | 172 | 8 | 1 | 461 | 1 | 462 | 0.0 | 556 |
| MsG0380014928.01.T01 | MTR_4g055170 | 48.718 | 468 | 212 | 12 | 7 | 464 | 9 | 458 | 3.14e-146 | 426 |
| MsG0380014928.01.T01 | MTR_2g015430 | 29.501 | 461 | 289 | 15 | 8 | 459 | 2 | 435 | 2.46e-58 | 199 |
| MsG0380014928.01.T01 | MTR_7g027175 | 30.043 | 466 | 272 | 16 | 3 | 456 | 10 | 433 | 5.85e-44 | 160 |
| MsG0380014928.01.T01 | MTR_1g053315 | 29.464 | 448 | 288 | 17 | 19 | 458 | 30 | 457 | 9.54e-40 | 149 |
| MsG0380014928.01.T01 | MTR_1g040175 | 31.594 | 345 | 214 | 11 | 10 | 348 | 9 | 337 | 1.07e-39 | 149 |
| MsG0380014928.01.T01 | MTR_8g096530 | 28.571 | 462 | 281 | 16 | 8 | 459 | 5 | 427 | 9.98e-38 | 143 |
| MsG0380014928.01.T01 | MTR_7g110610 | 31.940 | 335 | 170 | 9 | 17 | 348 | 12 | 291 | 1.88e-37 | 141 |
| MsG0380014928.01.T01 | MTR_3g071320 | 29.455 | 404 | 250 | 14 | 10 | 400 | 5 | 386 | 5.49e-37 | 141 |
| MsG0380014928.01.T01 | MTR_1g081660 | 26.247 | 461 | 304 | 15 | 8 | 461 | 16 | 447 | 1.95e-36 | 140 |
| MsG0380014928.01.T01 | MTR_3g098980 | 28.571 | 455 | 283 | 18 | 8 | 456 | 12 | 430 | 2.74e-36 | 139 |
| MsG0380014928.01.T01 | MTR_1g040215 | 29.462 | 353 | 223 | 8 | 4 | 348 | 3 | 337 | 1.56e-35 | 137 |
| MsG0380014928.01.T01 | MTR_5g076700 | 26.531 | 392 | 263 | 10 | 17 | 403 | 17 | 388 | 2.58e-35 | 137 |
| MsG0380014928.01.T01 | MTR_7g114520 | 27.412 | 456 | 290 | 16 | 17 | 461 | 18 | 443 | 1.07e-34 | 135 |
| MsG0380014928.01.T01 | MTR_7g114550 | 28.955 | 335 | 223 | 8 | 17 | 348 | 17 | 339 | 5.59e-33 | 130 |
| MsG0380014928.01.T01 | MTR_7g114470 | 26.582 | 395 | 264 | 12 | 17 | 405 | 18 | 392 | 1.32e-32 | 129 |
| MsG0380014928.01.T01 | MTR_7g114490 | 26.835 | 395 | 261 | 11 | 17 | 405 | 17 | 389 | 7.46e-32 | 127 |
| MsG0380014928.01.T01 | MTR_7g114560 | 27.761 | 335 | 227 | 7 | 17 | 348 | 17 | 339 | 1.29e-30 | 124 |
| MsG0380014928.01.T01 | MTR_2g105330 | 26.115 | 471 | 278 | 17 | 10 | 456 | 3 | 427 | 1.55e-29 | 120 |
| MsG0380014928.01.T01 | MTR_3g112180 | 26.271 | 472 | 252 | 19 | 18 | 455 | 20 | 429 | 1.70e-29 | 120 |
| MsG0380014928.01.T01 | MTR_2g064495 | 26.739 | 460 | 289 | 18 | 10 | 456 | 3 | 427 | 4.35e-29 | 119 |
| MsG0380014928.01.T01 | MTR_8g075610 | 28.177 | 362 | 228 | 12 | 17 | 369 | 1 | 339 | 5.51e-29 | 118 |
| MsG0380014928.01.T01 | MTR_4g007540 | 26.596 | 470 | 290 | 17 | 10 | 465 | 3 | 431 | 1.10e-27 | 115 |
| MsG0380014928.01.T01 | MTR_1g040315 | 26.741 | 359 | 229 | 12 | 4 | 352 | 3 | 337 | 3.19e-27 | 113 |
| MsG0380014928.01.T01 | MTR_4g127650 | 30.612 | 343 | 205 | 14 | 18 | 346 | 18 | 341 | 1.69e-26 | 112 |
| MsG0380014928.01.T01 | MTR_3g049150 | 26.812 | 414 | 244 | 18 | 18 | 400 | 10 | 395 | 1.06e-25 | 109 |
| MsG0380014928.01.T01 | MTR_3g026950 | 24.543 | 383 | 247 | 14 | 18 | 380 | 10 | 370 | 6.39e-25 | 107 |
| MsG0380014928.01.T01 | MTR_4g097090 | 27.859 | 341 | 220 | 12 | 18 | 347 | 17 | 342 | 7.68e-24 | 104 |
| MsG0380014928.01.T01 | MTR_1g040320 | 26.744 | 344 | 202 | 11 | 7 | 348 | 6 | 301 | 9.03e-24 | 103 |
| MsG0380014928.01.T01 | MTR_1g040195 | 25.885 | 452 | 275 | 16 | 17 | 461 | 16 | 414 | 1.06e-22 | 100 |
| MsG0380014928.01.T01 | MTR_7g086000 | 27.446 | 368 | 223 | 16 | 18 | 359 | 12 | 361 | 2.33e-22 | 99.8 |
| MsG0380014928.01.T01 | MTR_7g085930 | 27.964 | 329 | 197 | 14 | 69 | 372 | 59 | 372 | 2.58e-21 | 96.7 |
| MsG0380014928.01.T01 | MTR_0502s0010 | 28.514 | 249 | 156 | 7 | 110 | 348 | 2 | 238 | 2.40e-20 | 90.5 |
| MsG0380014928.01.T01 | MTR_4g013050 | 25.000 | 360 | 214 | 14 | 18 | 372 | 12 | 320 | 3.68e-20 | 92.8 |
| MsG0380014928.01.T01 | MTR_7g085990 | 27.829 | 327 | 200 | 13 | 69 | 372 | 55 | 368 | 1.46e-19 | 91.3 |
| MsG0380014928.01.T01 | MTR_3g102020 | 27.174 | 276 | 179 | 7 | 69 | 334 | 69 | 332 | 1.61e-17 | 85.1 |
| MsG0380014928.01.T01 | MTR_7g084940 | 25.272 | 459 | 297 | 19 | 8 | 455 | 14 | 437 | 1.27e-16 | 82.4 |
| MsG0380014928.01.T01 | MTR_3g101960 | 28.417 | 278 | 173 | 7 | 69 | 334 | 68 | 331 | 1.45e-16 | 82.0 |
| MsG0380014928.01.T01 | MTR_7g114510 | 25.316 | 316 | 206 | 10 | 154 | 461 | 8 | 301 | 2.88e-16 | 80.1 |
| MsG0380014928.01.T01 | MTR_0042s0190 | 28.333 | 180 | 119 | 5 | 172 | 348 | 1 | 173 | 2.51e-15 | 77.0 |
| MsG0380014928.01.T01 | MTR_7g056087 | 24.307 | 469 | 292 | 17 | 9 | 454 | 3 | 431 | 6.74e-15 | 77.0 |
| MsG0380014928.01.T01 | MTR_3g101900 | 23.977 | 342 | 224 | 9 | 10 | 334 | 7 | 329 | 1.28e-14 | 76.3 |
| MsG0380014928.01.T01 | MTR_3g101930 | 24.275 | 276 | 187 | 6 | 69 | 332 | 70 | 335 | 1.35e-13 | 73.2 |
| MsG0380014928.01.T01 | MTR_4g022825 | 24.354 | 271 | 194 | 6 | 71 | 334 | 57 | 323 | 3.76e-12 | 68.6 |
| MsG0380014928.01.T01 | MTR_5g090880 | 24.534 | 322 | 205 | 10 | 69 | 368 | 67 | 372 | 4.50e-12 | 68.2 |
| MsG0380014928.01.T01 | MTR_3g450710 | 23.602 | 322 | 209 | 9 | 69 | 368 | 65 | 371 | 5.38e-12 | 68.2 |
| MsG0380014928.01.T01 | MTR_4g022800 | 26.207 | 290 | 180 | 11 | 64 | 334 | 50 | 324 | 1.38e-11 | 66.6 |
| MsG0380014928.01.T01 | MTR_3g070170 | 24.534 | 322 | 181 | 10 | 69 | 348 | 66 | 367 | 2.69e-11 | 65.9 |
| MsG0380014928.01.T01 | MTR_5g026320 | 22.460 | 374 | 253 | 11 | 2 | 365 | 12 | 358 | 3.76e-11 | 65.5 |
| MsG0380014928.01.T01 | MTR_8g041770 | 24.354 | 271 | 185 | 7 | 69 | 332 | 64 | 321 | 3.98e-11 | 65.1 |
| MsG0380014928.01.T01 | MTR_7g084910 | 25.513 | 341 | 213 | 14 | 8 | 331 | 15 | 331 | 5.61e-11 | 64.7 |
| MsG0380014928.01.T01 | MTR_5g090830 | 22.769 | 325 | 206 | 11 | 69 | 368 | 66 | 370 | 9.38e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014928.01.T01 | AT5G07080 | 38.723 | 470 | 239 | 13 | 8 | 461 | 12 | 448 | 1.06e-97 | 301 |
| MsG0380014928.01.T01 | AT2G25150 | 38.889 | 468 | 254 | 13 | 8 | 465 | 11 | 456 | 3.73e-93 | 290 |
| MsG0380014928.01.T01 | AT2G23510 | 38.696 | 460 | 256 | 13 | 8 | 461 | 11 | 450 | 7.53e-89 | 278 |
| MsG0380014928.01.T01 | AT3G47170 | 37.155 | 471 | 260 | 12 | 10 | 461 | 12 | 465 | 3.07e-87 | 275 |
| MsG0380014928.01.T01 | AT1G28680 | 34.341 | 463 | 272 | 15 | 6 | 459 | 2 | 441 | 2.28e-74 | 241 |
| MsG0380014928.01.T01 | AT1G28680 | 36.096 | 374 | 212 | 13 | 6 | 370 | 2 | 357 | 6.00e-61 | 204 |
| MsG0380014928.01.T01 | AT5G41040 | 29.412 | 459 | 279 | 15 | 8 | 457 | 15 | 437 | 1.93e-47 | 169 |
| MsG0380014928.01.T01 | AT5G41040 | 29.412 | 459 | 279 | 15 | 8 | 457 | 31 | 453 | 2.02e-47 | 170 |
| MsG0380014928.01.T01 | AT3G48720 | 28.924 | 446 | 276 | 18 | 17 | 456 | 16 | 426 | 7.71e-43 | 157 |
| MsG0380014928.01.T01 | AT3G62160 | 30.206 | 437 | 253 | 16 | 16 | 444 | 11 | 403 | 1.71e-42 | 156 |
| MsG0380014928.01.T01 | AT3G03480 | 27.273 | 462 | 300 | 13 | 7 | 461 | 16 | 448 | 1.08e-36 | 140 |
| MsG0380014928.01.T01 | AT5G63560 | 29.455 | 404 | 250 | 15 | 8 | 405 | 7 | 381 | 2.41e-36 | 139 |
| MsG0380014928.01.T01 | AT5G17540 | 28.178 | 472 | 284 | 18 | 7 | 461 | 6 | 439 | 5.89e-35 | 136 |
| MsG0380014928.01.T01 | AT1G03390 | 27.442 | 430 | 275 | 15 | 20 | 436 | 32 | 437 | 1.04e-33 | 132 |
| MsG0380014928.01.T01 | AT2G40230 | 28.141 | 398 | 259 | 11 | 10 | 400 | 5 | 382 | 4.61e-32 | 127 |
| MsG0380014928.01.T01 | AT5G48930 | 27.970 | 404 | 258 | 15 | 8 | 400 | 1 | 382 | 4.26e-30 | 122 |
| MsG0380014928.01.T01 | AT1G27620 | 27.451 | 408 | 242 | 15 | 11 | 401 | 10 | 380 | 2.13e-29 | 120 |
| MsG0380014928.01.T01 | AT2G19070 | 26.522 | 460 | 289 | 16 | 18 | 455 | 12 | 444 | 3.42e-25 | 108 |
| MsG0380014928.01.T01 | AT5G57840 | 27.249 | 389 | 223 | 16 | 8 | 371 | 1 | 354 | 3.32e-21 | 96.3 |
| MsG0380014928.01.T01 | AT1G24430 | 30.982 | 326 | 182 | 19 | 17 | 334 | 12 | 302 | 1.92e-20 | 94.0 |
| MsG0380014928.01.T01 | AT4G31910 | 27.561 | 410 | 227 | 21 | 28 | 400 | 29 | 405 | 1.14e-19 | 91.7 |
| MsG0380014928.01.T01 | AT1G65450 | 27.970 | 404 | 248 | 17 | 8 | 395 | 16 | 392 | 2.06e-17 | 84.7 |
| MsG0380014928.01.T01 | AT5G23940 | 26.437 | 261 | 174 | 9 | 69 | 318 | 65 | 318 | 4.34e-13 | 71.6 |
| MsG0380014928.01.T01 | AT4G15390 | 23.876 | 356 | 228 | 13 | 4 | 348 | 2 | 325 | 7.46e-13 | 70.9 |
| MsG0380014928.01.T01 | AT4G15390 | 23.876 | 356 | 228 | 13 | 4 | 348 | 2 | 325 | 7.46e-13 | 70.9 |
| MsG0380014928.01.T01 | AT5G47950 | 32.161 | 199 | 110 | 9 | 7 | 199 | 3 | 182 | 1.20e-12 | 70.1 |
| MsG0380014928.01.T01 | AT3G26040 | 25.000 | 276 | 174 | 11 | 17 | 286 | 10 | 258 | 4.49e-12 | 68.2 |
Find 79 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCATTGCTACTAAGCTTTA+AGG | 0.103803 | 3:+62629018 | None:intergenic |
| TTTCACGAAAATATTGATTT+TGG | 0.166223 | 3:-62628692 | MsG0380014928.01.T01:CDS |
| AGGACGAAATTGAAGCTTTA+AGG | 0.184665 | 3:-62628505 | MsG0380014928.01.T01:CDS |
| AAGTTACCCTTTGGTGTTAA+TGG | 0.185564 | 3:-62629431 | MsG0380014928.01.T01:CDS |
| ACTTTACCTAAAGGGTATTA+TGG | 0.240183 | 3:-62628941 | MsG0380014928.01.T01:CDS |
| AGTTTGGACAATATTATTAT+TGG | 0.244046 | 3:+62630436 | None:intergenic |
| AATACCGACGGTGCAGAATT+TGG | 0.271742 | 3:-62629571 | MsG0380014928.01.T01:CDS |
| CCGATCATCATCAGCAATTC+AGG | 0.282349 | 3:-62630402 | MsG0380014928.01.T01:CDS |
| CTTTGTGGTGGTTTCACAAT+TGG | 0.302913 | 3:-62629397 | MsG0380014928.01.T01:CDS |
| AGAATTTGGCATTCCTTTCT+TGG | 0.315072 | 3:-62629557 | MsG0380014928.01.T01:CDS |
| CTAACTCCTCCTTCCATTGA+TGG | 0.334307 | 3:+62628569 | None:intergenic |
| TTTGACAGAGTGGAAGCATT+TGG | 0.351631 | 3:-62628717 | MsG0380014928.01.T01:CDS |
| AACTTAGGCATGGCATCAAC+AGG | 0.352804 | 3:+62628530 | None:intergenic |
| CACAGAAATTGCAAAACATT+TGG | 0.368692 | 3:-62629482 | MsG0380014928.01.T01:CDS |
| GAATTGCCATAATACCCTTT+AGG | 0.371067 | 3:+62628935 | None:intergenic |
| CATGCTATCTGTGACGGATT+TGG | 0.372536 | 3:-62629361 | MsG0380014928.01.T01:CDS |
| CTATCAACACTTGACAATAA+AGG | 0.389671 | 3:-62630461 | MsG0380014928.01.T01:CDS |
| TTGACAGAGTGGAAGCATTT+GGG | 0.391527 | 3:-62628716 | MsG0380014928.01.T01:CDS |
| GTATTATGGCAATTCTACTA+TGG | 0.400718 | 3:-62628927 | MsG0380014928.01.T01:CDS |
| ATTATGATACTTTACCTAAA+GGG | 0.416811 | 3:-62628949 | MsG0380014928.01.T01:CDS |
| GTTTCTTTACTCGAAGTTGT+AGG | 0.420009 | 3:-62628483 | None:intergenic |
| CATCAATGGAAGGAGGAGTT+AGG | 0.420940 | 3:-62628568 | MsG0380014928.01.T01:CDS |
| TGGTATGAGACGTAACTTGA+TGG | 0.424968 | 3:-62628972 | MsG0380014928.01.T01:CDS |
| TCAAGATAAAAGTTACCCTT+TGG | 0.428708 | 3:-62629440 | MsG0380014928.01.T01:CDS |
| TGATGCCATGCCTAAGTTCA+AGG | 0.446582 | 3:-62628525 | MsG0380014928.01.T01:CDS |
| TTAGCAGTTGCTTCCAAGAA+AGG | 0.451873 | 3:+62629544 | None:intergenic |
| ACTTTCTTCCCTTCATCACC+TGG | 0.454187 | 3:-62629515 | MsG0380014928.01.T01:CDS |
| GGTGGTTTCACAATTGGAAT+GGG | 0.460166 | 3:-62629391 | MsG0380014928.01.T01:CDS |
| TCAATTTCGTCCTTGAACTT+AGG | 0.461185 | 3:+62628515 | None:intergenic |
| TGCAACAGATTCTCTGTCCA+TGG | 0.465029 | 3:+62629213 | None:intergenic |
| GATTATGATACTTTACCTAA+AGG | 0.466862 | 3:-62628950 | MsG0380014928.01.T01:CDS |
| ATCGGTATACATGAACAGTT+TGG | 0.466930 | 3:+62630420 | None:intergenic |
| TTGTCACCATTAACACCAAA+GGG | 0.469934 | 3:+62629425 | None:intergenic |
| TCCCACACAGGTTTCACTGA+TGG | 0.471401 | 3:+62629286 | None:intergenic |
| TGGTGGTTTCACAATTGGAA+TGG | 0.478781 | 3:-62629392 | MsG0380014928.01.T01:CDS |
| TCCATGGGGCATTGTGGAAA+AGG | 0.482880 | 3:+62629229 | None:intergenic |
| GGTTGCTTTCTCATATTCCA+TGG | 0.492823 | 3:+62630553 | None:intergenic |
| ACAATGCTAAATATATTAGT+TGG | 0.493633 | 3:-62628992 | MsG0380014928.01.T01:CDS |
| CGATAATCATGATCCATCAA+TGG | 0.498996 | 3:-62628582 | MsG0380014928.01.T01:CDS |
| AGAAAGAATACAAGAAGGAG+TGG | 0.500364 | 3:+62630484 | None:intergenic |
| TTTCTGTGTTAGTATTGTCC+AGG | 0.504574 | 3:+62629497 | None:intergenic |
| TAGTATTGTCCAGGTGATGA+AGG | 0.508869 | 3:+62629506 | None:intergenic |
| GATAGAGAAAGAATACAAGA+AGG | 0.520122 | 3:+62630479 | None:intergenic |
| AGCAACCCTAAAGATTGAAA+AGG | 0.526012 | 3:-62630537 | MsG0380014928.01.T01:CDS |
| ATAATAATAAAACAAAGCCT+TGG | 0.539420 | 3:+62630331 | None:intergenic |
| AGCTTAACAATTTCATAGAG+TGG | 0.539783 | 3:+62628863 | None:intergenic |
| GCAACAGATTCTCTGTCCAT+GGG | 0.542222 | 3:+62629214 | None:intergenic |
| CTCCTCTTGATTTGTCTCCA+CGG | 0.547932 | 3:+62628781 | None:intergenic |
| TTTGTCACCATTAACACCAA+AGG | 0.555424 | 3:+62629424 | None:intergenic |
| TTCGTCCTTGAACTTAGGCA+TGG | 0.563446 | 3:+62628520 | None:intergenic |
| GTGACAAAATTTCTTTGTGG+TGG | 0.567922 | 3:-62629409 | MsG0380014928.01.T01:CDS |
| GCAGTTGTTGAACTTGCAAG+AGG | 0.580378 | 3:-62629319 | MsG0380014928.01.T01:CDS |
| ATGGTGACAAAATTTCTTTG+TGG | 0.580801 | 3:-62629412 | MsG0380014928.01.T01:CDS |
| ATTCAAAGAAGCGTTGTCCA+AGG | 0.592306 | 3:-62630348 | MsG0380014928.01.T01:CDS |
| TCTCTGTCCATGGGGCATTG+TGG | 0.602929 | 3:+62629223 | None:intergenic |
| ATGTCACATGCTATCTGTGA+CGG | 0.605048 | 3:-62629367 | MsG0380014928.01.T01:CDS |
| AGTATTGTCCAGGTGATGAA+GGG | 0.605282 | 3:+62629507 | None:intergenic |
| AAGTGCTTGCAGGTTATGCG+TGG | 0.605328 | 3:-62629057 | MsG0380014928.01.T01:CDS |
| TGAATTAGTAACATAATCAG+TGG | 0.606396 | 3:+62628811 | None:intergenic |
| CTTCTTTGAATATATGACAA+GGG | 0.606817 | 3:+62630360 | None:intergenic |
| AAAACTCGTATACCTGCTAG+AGG | 0.608559 | 3:+62630308 | None:intergenic |
| CCTGAATTGCTGATGATGAT+CGG | 0.617513 | 3:+62630402 | None:intergenic |
| AATCATGATCCATCAATGGA+AGG | 0.619997 | 3:-62628578 | MsG0380014928.01.T01:CDS |
| CTCCGTGGAGACAAATCAAG+AGG | 0.634372 | 3:-62628783 | MsG0380014928.01.T01:CDS |
| TCAAGAGGAGGATTTCAGCA+TGG | 0.634848 | 3:-62628768 | MsG0380014928.01.T01:CDS |
| AATGCCAAATTCTGCACCGT+CGG | 0.636963 | 3:+62629567 | None:intergenic |
| TACTAATTCAATAAACTCCG+TGG | 0.637783 | 3:-62628798 | MsG0380014928.01.T01:CDS |
| GTGGTTTCACAATTGGAATG+GGG | 0.639088 | 3:-62629390 | MsG0380014928.01.T01:CDS |
| AGACTAGTGAGACATGCTGA+TGG | 0.640547 | 3:-62629613 | MsG0380014928.01.T01:CDS |
| GCTTCTTTGAATATATGACA+AGG | 0.640717 | 3:+62630359 | None:intergenic |
| TGCTTGCAGGTTATGCGTGG+AGG | 0.643318 | 3:-62629054 | MsG0380014928.01.T01:CDS |
| CATGATCCATCAATGGAAGG+AGG | 0.648343 | 3:-62628575 | MsG0380014928.01.T01:CDS |
| CGTGGAGACAAATCAAGAGG+AGG | 0.666183 | 3:-62628780 | MsG0380014928.01.T01:CDS |
| ACTTAGGCATGGCATCAACA+GGG | 0.673752 | 3:+62628531 | None:intergenic |
| AGCATGGAATTAGAATCTAG+TGG | 0.674836 | 3:-62628752 | MsG0380014928.01.T01:CDS |
| AGCCATCAGTGAAACCTGTG+TGG | 0.685980 | 3:-62629288 | MsG0380014928.01.T01:CDS |
| CAACAGATTCTCTGTCCATG+GGG | 0.687729 | 3:+62629215 | None:intergenic |
| AGAGTCAAGATCAATACCGA+CGG | 0.695857 | 3:-62629583 | MsG0380014928.01.T01:CDS |
| GCCATCAGTGAAACCTGTGT+GGG | 0.717270 | 3:-62629287 | MsG0380014928.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATAAAAATGAAAAAAT+AGG | - | Chr3:62629941-62629960 | MsG0380014928.01.T01:intron | 10.0% |
| !! | TGAATAAAAATGAAAAAATA+GGG | - | Chr3:62629942-62629961 | MsG0380014928.01.T01:intron | 10.0% |
| !! | AGTTGTTCTTAAATAAAATA+AGG | + | Chr3:62629120-62629139 | None:intergenic | 15.0% |
| !!! | ACATTAACTTTTTTCATATA+GGG | - | Chr3:62628898-62628917 | MsG0380014928.01.T01:CDS | 15.0% |
| !!! | GTAATTATTAGACATTTTTA+CGG | - | Chr3:62629144-62629163 | MsG0380014928.01.T01:CDS | 15.0% |
| !! | AACTAGTTGTTAAACATTTA+GGG | + | Chr3:62628777-62628796 | None:intergenic | 20.0% |
| !! | ACAATAAGTAAGTATATTGA+GGG | - | Chr3:62628961-62628980 | MsG0380014928.01.T01:CDS | 20.0% |
| !! | ACAATGCTAAATATATTAGT+TGG | - | Chr3:62630043-62630062 | MsG0380014928.01.T01:intron | 20.0% |
| !! | AGTTTGGACAATATTATTAT+TGG | + | Chr3:62628602-62628621 | None:intergenic | 20.0% |
| !! | ATAATAATAAAACAAAGCCT+TGG | + | Chr3:62628707-62628726 | None:intergenic | 20.0% |
| !! | ATTATGATACTTTACCTAAA+GGG | - | Chr3:62630086-62630105 | MsG0380014928.01.T01:intron | 20.0% |
| !! | GATAATATGTAAGAACTTAT+AGG | - | Chr3:62628929-62628948 | MsG0380014928.01.T01:CDS | 20.0% |
| !! | TAACTAGTTGTTAAACATTT+AGG | + | Chr3:62628778-62628797 | None:intergenic | 20.0% |
| !!! | AGTATTAGTTTTATTCACAA+AGG | + | Chr3:62629855-62629874 | None:intergenic | 20.0% |
| !!! | CAATATTGTTTTAAAGTAGA+GGG | - | Chr3:62629887-62629906 | MsG0380014928.01.T01:intron | 20.0% |
| !!! | GACATTAACTTTTTTCATAT+AGG | - | Chr3:62628897-62628916 | MsG0380014928.01.T01:CDS | 20.0% |
| !!! | GTATTAGTTTTATTCACAAA+GGG | + | Chr3:62629854-62629873 | None:intergenic | 20.0% |
| !!! | TTTCACGAAAATATTGATTT+TGG | - | Chr3:62630343-62630362 | MsG0380014928.01.T01:CDS | 20.0% |
| ! | AAAGTTAATGTCAACATTGA+TGG | + | Chr3:62628889-62628908 | None:intergenic | 25.0% |
| ! | CTTCTTTGAATATATGACAA+GGG | + | Chr3:62628678-62628697 | None:intergenic | 25.0% |
| ! | GACAATAAGTAAGTATATTG+AGG | - | Chr3:62628960-62628979 | MsG0380014928.01.T01:CDS | 25.0% |
| ! | GATTATGATACTTTACCTAA+AGG | - | Chr3:62630085-62630104 | MsG0380014928.01.T01:intron | 25.0% |
| ! | TATACGACTCAACAAAAATA+TGG | + | Chr3:62629244-62629263 | None:intergenic | 25.0% |
| ! | TGAATTAGTAACATAATCAG+TGG | + | Chr3:62630227-62630246 | None:intergenic | 25.0% |
| !! | ATCTTCCTTTTCAATCTTTA+GGG | + | Chr3:62628506-62628525 | None:intergenic | 25.0% |
| !! | TTTTATTATTATCCTCTAGC+AGG | - | Chr3:62628715-62628734 | MsG0380014928.01.T01:CDS | 25.0% |
| !!! | AAGTAATTAGTTTGTTCGTT+AGG | + | Chr3:62629328-62629347 | None:intergenic | 25.0% |
| !!! | AGGTATACGAGTTTTTTTTA+TGG | - | Chr3:62628735-62628754 | MsG0380014928.01.T01:CDS | 25.0% |
| !!! | ATACTATTTTGAGACTATTG+TGG | - | Chr3:62629055-62629074 | MsG0380014928.01.T01:CDS | 25.0% |
| !!! | GCAATATTGTTTTAAAGTAG+AGG | - | Chr3:62629886-62629905 | MsG0380014928.01.T01:intron | 25.0% |
| !!! | GGTATACGAGTTTTTTTTAT+GGG | - | Chr3:62628736-62628755 | MsG0380014928.01.T01:CDS | 25.0% |
| AGCTTAACAATTTCATAGAG+TGG | + | Chr3:62630175-62630194 | None:intergenic | 30.0% | |
| ATGGTGACAAAATTTCTTTG+TGG | - | Chr3:62629623-62629642 | MsG0380014928.01.T01:intron | 30.0% | |
| ATTGGTGCAATTAAAGATTG+TGG | + | Chr3:62629383-62629402 | None:intergenic | 30.0% | |
| CACAGAAATTGCAAAACATT+TGG | - | Chr3:62629553-62629572 | MsG0380014928.01.T01:CDS | 30.0% | |
| CTATCAACACTTGACAATAA+AGG | - | Chr3:62628574-62628593 | MsG0380014928.01.T01:CDS | 30.0% | |
| GATAGAGAAAGAATACAAGA+AGG | + | Chr3:62628559-62628578 | None:intergenic | 30.0% | |
| GCTTCTTTGAATATATGACA+AGG | + | Chr3:62628679-62628698 | None:intergenic | 30.0% | |
| GTATTATGGCAATTCTACTA+TGG | - | Chr3:62630108-62630127 | MsG0380014928.01.T01:intron | 30.0% | |
| TACTAATTCAATAAACTCCG+TGG | - | Chr3:62630237-62630256 | MsG0380014928.01.T01:intron | 30.0% | |
| TCAAGATAAAAGTTACCCTT+TGG | - | Chr3:62629595-62629614 | MsG0380014928.01.T01:CDS | 30.0% | |
| ! | AAAGGGTAACTTTTATCTTG+AGG | + | Chr3:62629596-62629615 | None:intergenic | 30.0% |
| ! | CATCTTCCTTTTCAATCTTT+AGG | + | Chr3:62628507-62628526 | None:intergenic | 30.0% |
| ! | TAACTTTTATCTTGAGGAGA+AGG | + | Chr3:62629590-62629609 | None:intergenic | 30.0% |
| ! | TTTTATGGGTAGCAATGATT+TGG | - | Chr3:62628750-62628769 | MsG0380014928.01.T01:CDS | 30.0% |
| !! | ACTTTACCTAAAGGGTATTA+TGG | - | Chr3:62630094-62630113 | MsG0380014928.01.T01:intron | 30.0% |
| !!! | CACGAAAATATTGATTTTGG+AGG | - | Chr3:62630346-62630365 | MsG0380014928.01.T01:CDS | 30.0% |
| !!! | GATGGACTATTTTAATGTCA+GGG | + | Chr3:62628871-62628890 | None:intergenic | 30.0% |
| !!! | TGATGGACTATTTTAATGTC+AGG | + | Chr3:62628872-62628891 | None:intergenic | 30.0% |
| AATCATGATCCATCAATGGA+AGG | - | Chr3:62630457-62630476 | MsG0380014928.01.T01:CDS | 35.0% | |
| AGAAAGAATACAAGAAGGAG+TGG | + | Chr3:62628554-62628573 | None:intergenic | 35.0% | |
| AGAATTTGGCATTCCTTTCT+TGG | - | Chr3:62629478-62629497 | MsG0380014928.01.T01:CDS | 35.0% | |
| AGCAACCCTAAAGATTGAAA+AGG | - | Chr3:62628498-62628517 | MsG0380014928.01.T01:CDS | 35.0% | |
| AGCATGGAATTAGAATCTAG+TGG | - | Chr3:62630283-62630302 | MsG0380014928.01.T01:intron | 35.0% | |
| AGGACGAAATTGAAGCTTTA+AGG | - | Chr3:62630530-62630549 | MsG0380014928.01.T01:CDS | 35.0% | |
| ATCGGTATACATGAACAGTT+TGG | + | Chr3:62628618-62628637 | None:intergenic | 35.0% | |
| CGATAATCATGATCCATCAA+TGG | - | Chr3:62630453-62630472 | MsG0380014928.01.T01:CDS | 35.0% | |
| GAATTGCCATAATACCCTTT+AGG | + | Chr3:62630103-62630122 | None:intergenic | 35.0% | |
| GTCATTGCTACTAAGCTTTA+AGG | + | Chr3:62630020-62630039 | None:intergenic | 35.0% | |
| GTGACAAAATTTCTTTGTGG+TGG | - | Chr3:62629626-62629645 | MsG0380014928.01.T01:intron | 35.0% | |
| TCAATTTCGTCCTTGAACTT+AGG | + | Chr3:62630523-62630542 | None:intergenic | 35.0% | |
| TGGGAAAGAGAAAAACAAGT+TGG | - | Chr3:62629767-62629786 | MsG0380014928.01.T01:intron | 35.0% | |
| TTGTCACCATTAACACCAAA+GGG | + | Chr3:62629613-62629632 | None:intergenic | 35.0% | |
| TTTCTGTGTTAGTATTGTCC+AGG | + | Chr3:62629541-62629560 | None:intergenic | 35.0% | |
| TTTGTCACCATTAACACCAA+AGG | + | Chr3:62629614-62629633 | None:intergenic | 35.0% | |
| !! | AAGTTACCCTTTGGTGTTAA+TGG | - | Chr3:62629604-62629623 | MsG0380014928.01.T01:CDS | 35.0% |
| AAAACTCGTATACCTGCTAG+AGG | + | Chr3:62628730-62628749 | None:intergenic | 40.0% | |
| AGAGTCAAGATCAATACCGA+CGG | - | Chr3:62629452-62629471 | MsG0380014928.01.T01:CDS | 40.0% | |
| AGTATTGTCCAGGTGATGAA+GGG | + | Chr3:62629531-62629550 | None:intergenic | 40.0% | |
| ATGTCACATGCTATCTGTGA+CGG | - | Chr3:62629668-62629687 | MsG0380014928.01.T01:intron | 40.0% | |
| CTTTGTGGTGGTTTCACAAT+TGG | - | Chr3:62629638-62629657 | MsG0380014928.01.T01:intron | 40.0% | |
| GGTGGTTTCACAATTGGAAT+GGG | - | Chr3:62629644-62629663 | MsG0380014928.01.T01:intron | 40.0% | |
| GTGGTTTCACAATTGGAATG+GGG | - | Chr3:62629645-62629664 | MsG0380014928.01.T01:intron | 40.0% | |
| TAGTATTGTCCAGGTGATGA+AGG | + | Chr3:62629532-62629551 | None:intergenic | 40.0% | |
| TGGTATGAGACGTAACTTGA+TGG | - | Chr3:62630063-62630082 | MsG0380014928.01.T01:intron | 40.0% | |
| TGGTGGTTTCACAATTGGAA+TGG | - | Chr3:62629643-62629662 | MsG0380014928.01.T01:intron | 40.0% | |
| TTAGCAGTTGCTTCCAAGAA+AGG | + | Chr3:62629494-62629513 | None:intergenic | 40.0% | |
| ! | ACAGTAGCAAGCATTTTGCA+TGG | + | Chr3:62630400-62630419 | None:intergenic | 40.0% |
| ! | CCTGAATTGCTGATGATGAT+CGG | + | Chr3:62628636-62628655 | None:intergenic | 40.0% |
| ! | TGTTTTTCTCTTTCCCACAC+AGG | + | Chr3:62629764-62629783 | None:intergenic | 40.0% |
| !! | ATTCAAAGAAGCGTTGTCCA+AGG | - | Chr3:62628687-62628706 | MsG0380014928.01.T01:CDS | 40.0% |
| !! | CAAGAAGGAGTGGATTTTGA+TGG | + | Chr3:62628544-62628563 | None:intergenic | 40.0% |
| !! | CAGTAACAGTTTTGACAGAG+TGG | - | Chr3:62630308-62630327 | MsG0380014928.01.T01:intron | 40.0% |
| !! | TTGACAGAGTGGAAGCATTT+GGG | - | Chr3:62630319-62630338 | MsG0380014928.01.T01:intron | 40.0% |
| !! | TTTGACAGAGTGGAAGCATT+TGG | - | Chr3:62630318-62630337 | MsG0380014928.01.T01:intron | 40.0% |
| !!! | ACAACTTTTGAAGTGCTTGC+AGG | - | Chr3:62629968-62629987 | MsG0380014928.01.T01:intron | 40.0% |
| AACTTAGGCATGGCATCAAC+AGG | + | Chr3:62630508-62630527 | None:intergenic | 45.0% | |
| AATACCGACGGTGCAGAATT+TGG | - | Chr3:62629464-62629483 | MsG0380014928.01.T01:CDS | 45.0% | |
| AATGCCAAATTCTGCACCGT+CGG | + | Chr3:62629471-62629490 | None:intergenic | 45.0% | |
| ACTTAGGCATGGCATCAACA+GGG | + | Chr3:62630507-62630526 | None:intergenic | 45.0% | |
| ACTTTCTTCCCTTCATCACC+TGG | - | Chr3:62629520-62629539 | MsG0380014928.01.T01:CDS | 45.0% | |
| AGACTAGTGAGACATGCTGA+TGG | - | Chr3:62629422-62629441 | MsG0380014928.01.T01:CDS | 45.0% | |
| CAACAGATTCTCTGTCCATG+GGG | + | Chr3:62629823-62629842 | None:intergenic | 45.0% | |
| CATCAATGGAAGGAGGAGTT+AGG | - | Chr3:62630467-62630486 | MsG0380014928.01.T01:CDS | 45.0% | |
| CATGATCCATCAATGGAAGG+AGG | - | Chr3:62630460-62630479 | MsG0380014928.01.T01:CDS | 45.0% | |
| CCGATCATCATCAGCAATTC+AGG | - | Chr3:62628633-62628652 | MsG0380014928.01.T01:CDS | 45.0% | |
| CCTGCAAAACAAGCGAAGAT+TGG | + | Chr3:62629401-62629420 | None:intergenic | 45.0% | |
| CTAACTCCTCCTTCCATTGA+TGG | + | Chr3:62630469-62630488 | None:intergenic | 45.0% | |
| GCAACAGATTCTCTGTCCAT+GGG | + | Chr3:62629824-62629843 | None:intergenic | 45.0% | |
| GCAGTTGTTGAACTTGCAAG+AGG | - | Chr3:62629716-62629735 | MsG0380014928.01.T01:intron | 45.0% | |
| TCAAGAGGAGGATTTCAGCA+TGG | - | Chr3:62630267-62630286 | MsG0380014928.01.T01:intron | 45.0% | |
| TGATGCCATGCCTAAGTTCA+AGG | - | Chr3:62630510-62630529 | MsG0380014928.01.T01:CDS | 45.0% | |
| TGCAACAGATTCTCTGTCCA+TGG | + | Chr3:62629825-62629844 | None:intergenic | 45.0% | |
| TTCGTCCTTGAACTTAGGCA+TGG | + | Chr3:62630518-62630537 | None:intergenic | 45.0% | |
| !! | CATGCTATCTGTGACGGATT+TGG | - | Chr3:62629674-62629693 | MsG0380014928.01.T01:intron | 45.0% |
| !! | CTCCTCTTGATTTGTCTCCA+CGG | + | Chr3:62630257-62630276 | None:intergenic | 45.0% |
| !!! | CCAATCTTCGCTTGTTTTGC+AGG | - | Chr3:62629398-62629417 | MsG0380014928.01.T01:CDS | 45.0% |
| AAGTGCTTGCAGGTTATGCG+TGG | - | Chr3:62629978-62629997 | MsG0380014928.01.T01:intron | 50.0% | |
| AGCCATCAGTGAAACCTGTG+TGG | - | Chr3:62629747-62629766 | MsG0380014928.01.T01:intron | 50.0% | |
| CGTGGAGACAAATCAAGAGG+AGG | - | Chr3:62630255-62630274 | MsG0380014928.01.T01:intron | 50.0% | |
| CTCCGTGGAGACAAATCAAG+AGG | - | Chr3:62630252-62630271 | MsG0380014928.01.T01:intron | 50.0% | |
| GCCATCAGTGAAACCTGTGT+GGG | - | Chr3:62629748-62629767 | MsG0380014928.01.T01:intron | 50.0% | |
| TCCCACACAGGTTTCACTGA+TGG | + | Chr3:62629752-62629771 | None:intergenic | 50.0% | |
| ! | ACCTTTTCCACAATGCCCCA+TGG | - | Chr3:62629805-62629824 | MsG0380014928.01.T01:intron | 50.0% |
| ! | GCAAGCATTTTGCATGGTGC+TGG | + | Chr3:62630394-62630413 | None:intergenic | 50.0% |
| !! | TCCATGGGGCATTGTGGAAA+AGG | + | Chr3:62629809-62629828 | None:intergenic | 50.0% |
| TGCTTGCAGGTTATGCGTGG+AGG | - | Chr3:62629981-62630000 | MsG0380014928.01.T01:intron | 55.0% | |
| !! | TCTCTGTCCATGGGGCATTG+TGG | + | Chr3:62629815-62629834 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 62628484 | 62630573 | 62628484 | ID=MsG0380014928.01;Name=MsG0380014928.01 |
| Chr3 | mRNA | 62628484 | 62630573 | 62628484 | ID=MsG0380014928.01.T01;Parent=MsG0380014928.01;Name=MsG0380014928.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|468 |
| Chr3 | exon | 62630321 | 62630573 | 62630321 | ID=MsG0380014928.01.T01:exon:29547;Parent=MsG0380014928.01.T01 |
| Chr3 | exon | 62628484 | 62629637 | 62628484 | ID=MsG0380014928.01.T01:exon:29546;Parent=MsG0380014928.01.T01 |
| Chr3 | CDS | 62630321 | 62630573 | 62630321 | ID=MsG0380014928.01.T01:cds;Parent=MsG0380014928.01.T01 |
| Chr3 | CDS | 62628484 | 62629637 | 62628484 | ID=MsG0380014928.01.T01:cds;Parent=MsG0380014928.01.T01 |
| Gene Sequence |
| Protein sequence |
