AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019047.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019047.01.T01 MTR_4g022825 93.864 440 27 0 1 440 1 440 0.0 860
MsG0480019047.01.T01 MTR_4g022800 73.303 442 116 2 1 440 1 442 0.0 683
MsG0480019047.01.T01 MTR_4g022720 73.923 441 108 2 1 440 1 435 0.0 682
MsG0480019047.01.T01 MTR_4g022670 73.636 440 115 1 1 439 1 440 0.0 679
MsG0480019047.01.T01 MTR_7g026860 72.336 441 118 2 1 440 1 438 0.0 667
MsG0480019047.01.T01 MTR_3g088420 67.500 440 136 1 1 440 1 433 0.0 617
MsG0480019047.01.T01 MTR_3g026310 65.101 447 148 3 1 439 1 447 0.0 585
MsG0480019047.01.T01 MTR_4g022760 64.853 441 110 2 1 440 1 397 0.0 579
MsG0480019047.01.T01 MTR_8g041770 58.889 450 164 6 1 439 1 440 0.0 535
MsG0480019047.01.T01 MTR_0190s0020 57.206 451 173 7 1 440 1 442 0.0 519
MsG0480019047.01.T01 MTR_4g094835 55.165 455 172 9 1 436 1 442 5.66e-171 487
MsG0480019047.01.T01 MTR_4g094842 56.044 455 174 8 1 438 1 446 1.49e-168 481
MsG0480019047.01.T01 MTR_4g094838 51.754 456 169 10 4 436 1 428 2.82e-154 444
MsG0480019047.01.T01 MTR_4g094828 50.763 459 196 12 1 439 1 449 5.37e-147 426
MsG0480019047.01.T01 MTR_4g094832 56.283 382 141 6 69 436 57 426 7.60e-146 422
MsG0480019047.01.T01 MTR_8g079710 51.092 458 191 12 1 440 1 443 1.93e-144 419
MsG0480019047.01.T01 MTR_6g016815 52.500 440 136 7 1 440 15 381 1.20e-142 412
MsG0480019047.01.T01 MTR_4g022290 50.763 459 187 13 1 437 1 442 2.24e-140 409
MsG0480019047.01.T01 MTR_3g101900 38.964 444 243 12 12 440 18 448 3.14e-92 286
MsG0480019047.01.T01 MTR_8g040760 63.208 212 72 2 1 206 1 212 1.59e-89 271
MsG0480019047.01.T01 MTR_3g102020 38.409 440 243 10 15 437 20 448 1.43e-88 277
MsG0480019047.01.T01 MTR_3g101930 37.615 436 241 10 24 439 30 454 9.17e-87 272
MsG0480019047.01.T01 MTR_3g101970 37.004 454 247 11 3 439 5 436 1.47e-86 271
MsG0480019047.01.T01 MTR_3g101960 37.529 437 246 9 15 435 20 445 2.73e-86 271
MsG0480019047.01.T01 MTR_8g040780 59.722 216 83 2 224 435 12 227 1.09e-84 260
MsG0480019047.01.T01 MTR_3g102010 37.644 433 243 10 24 440 30 451 8.79e-84 265
MsG0480019047.01.T01 MTR_7g026850 70.060 167 49 1 29 194 12 178 1.55e-81 249
MsG0480019047.01.T01 MTR_7g026770 59.474 190 48 2 141 329 132 293 1.58e-70 225
MsG0480019047.01.T01 MTR_3g070170 32.700 474 261 12 3 436 11 466 8.34e-63 210
MsG0480019047.01.T01 MTR_4g015180 32.784 485 248 11 16 439 19 486 3.60e-61 206
MsG0480019047.01.T01 MTR_5g081960 31.809 481 251 10 16 436 19 482 5.41e-61 206
MsG0480019047.01.T01 MTR_6g045553 30.820 451 276 10 16 436 27 471 1.81e-54 188
MsG0480019047.01.T01 MTR_4g094825 48.000 200 96 5 5 198 72 269 7.74e-54 184
MsG0480019047.01.T01 MTR_4g094825 57.823 147 49 2 292 436 252 387 9.94e-49 171
MsG0480019047.01.T01 MTR_3g102030 34.713 314 164 7 87 396 44 320 1.58e-50 174
MsG0480019047.01.T01 MTR_6g034495 61.194 134 42 3 57 180 31 164 2.20e-48 163
MsG0480019047.01.T01 MTR_6g034485 57.576 132 48 2 188 319 2 125 1.17e-45 154
MsG0480019047.01.T01 MTR_7g026800 50.292 171 54 4 211 379 19 160 1.39e-38 138
MsG0480019047.01.T01 MTR_4g022780 49.133 173 57 4 211 381 20 163 4.09e-38 136
MsG0480019047.01.T01 MTR_7g026830 71.429 84 24 0 82 165 14 97 1.35e-36 130
MsG0480019047.01.T01 MTR_7g026780 56.364 110 24 2 331 440 26 111 7.27e-31 115
MsG0480019047.01.T01 MTR_8g096530 30.312 353 213 12 49 391 53 382 3.65e-30 122
MsG0480019047.01.T01 MTR_8g075610 27.226 393 242 16 55 429 47 413 1.07e-28 117
MsG0480019047.01.T01 MTR_4g007540 30.084 359 215 16 55 398 56 393 4.84e-28 115
MsG0480019047.01.T01 MTR_3g098980 27.704 379 236 13 55 421 66 418 1.76e-27 114
MsG0480019047.01.T01 MTR_2g064495 27.604 384 241 15 55 422 56 418 1.30e-26 111
MsG0480019047.01.T01 MTR_2g105330 27.344 384 242 15 55 422 56 418 1.30e-26 111
MsG0480019047.01.T01 MTR_3g026950 26.562 384 209 16 52 392 51 404 9.64e-23 100
MsG0480019047.01.T01 MTR_1g081660 26.303 403 226 15 24 392 31 396 1.58e-22 99.8
MsG0480019047.01.T01 MTR_7g014250 25.280 447 277 16 22 431 24 450 3.95e-21 95.9
MsG0480019047.01.T01 MTR_4g013050 26.287 369 187 12 53 392 51 363 4.97e-21 95.1
MsG0480019047.01.T01 MTR_3g071320 25.115 434 291 14 17 436 19 432 8.38e-21 94.7
MsG0480019047.01.T01 MTR_2g089650 24.638 414 265 18 38 417 43 443 1.94e-20 94.0
MsG0480019047.01.T01 MTR_7g014330 25.412 425 260 16 18 405 20 424 4.06e-20 92.8
MsG0480019047.01.T01 MTR_7g026870 74.074 54 14 0 297 350 98 151 9.29e-20 85.9
MsG0480019047.01.T01 MTR_7g015860 24.029 412 248 14 22 392 26 413 6.44e-19 89.4
MsG0480019047.01.T01 MTR_7g114520 24.722 360 240 13 44 392 53 392 1.48e-18 88.2
MsG0480019047.01.T01 MTR_3g086020 26.076 395 243 16 55 440 61 415 1.54e-18 87.8
MsG0480019047.01.T01 MTR_5g076700 23.832 428 272 16 25 422 24 427 1.97e-18 87.8
MsG0480019047.01.T01 MTR_3g112180 25.000 444 273 19 9 427 18 426 2.05e-18 87.8
MsG0480019047.01.T01 MTR_7g114550 23.583 441 284 16 25 436 24 440 3.42e-18 87.0
MsG0480019047.01.T01 MTR_3g049150 24.823 282 171 8 138 391 131 399 3.66e-18 86.7
MsG0480019047.01.T01 MTR_7g026880 68.627 51 16 0 389 439 2 52 4.01e-18 78.2
MsG0480019047.01.T01 MTR_7g014230 25.576 434 248 15 18 405 22 426 1.28e-17 85.1
MsG0480019047.01.T01 MTR_1g053315 24.048 420 256 18 33 422 60 446 1.40e-17 85.1
MsG0480019047.01.T01 MTR_4g097090 24.079 407 273 13 55 439 65 457 2.30e-17 84.3
MsG0480019047.01.T01 MTR_6g015320 25.641 312 202 10 137 427 142 444 4.88e-17 83.6
MsG0480019047.01.T01 MTR_7g114470 23.898 431 269 18 25 422 25 429 6.47e-17 83.2
MsG0480019047.01.T01 MTR_7g014200 23.025 443 292 12 22 427 28 458 1.08e-16 82.4
MsG0480019047.01.T01 MTR_7g014160 25.945 397 254 12 55 421 69 455 1.19e-16 82.4
MsG0480019047.01.T01 MTR_2g089765 24.757 412 242 14 26 391 45 434 2.44e-16 81.3
MsG0480019047.01.T01 MTR_7g084940 26.950 282 179 10 53 323 67 332 2.46e-16 81.3
MsG0480019047.01.T01 MTR_2g089735 25.708 424 255 17 41 424 43 446 2.55e-16 81.3
MsG0480019047.01.T01 MTR_7g027175 24.870 386 253 14 55 427 68 429 3.54e-16 80.5
MsG0480019047.01.T01 MTR_2g089670 23.981 417 242 16 21 391 42 429 3.88e-16 80.9
MsG0480019047.01.T01 MTR_6g015315 24.684 316 207 10 137 430 139 445 4.82e-16 80.5
MsG0480019047.01.T01 MTR_4g055170 27.815 302 197 11 55 350 69 355 1.07e-15 79.3
MsG0480019047.01.T01 MTR_6g015830 25.455 440 287 15 17 429 27 452 1.21e-15 79.3
MsG0480019047.01.T01 MTR_8g012750 23.515 404 270 10 22 391 24 422 1.27e-15 79.3
MsG0480019047.01.T01 MTR_6g015815 21.719 442 291 14 22 429 32 452 2.47e-15 78.2
MsG0480019047.01.T01 MTR_7g084910 27.407 270 162 9 53 307 68 318 4.66e-15 77.4
MsG0480019047.01.T01 MTR_7g085930 26.523 279 186 6 43 308 47 319 7.16e-15 76.6
MsG0480019047.01.T01 MTR_6g015850 23.611 432 290 12 22 428 32 448 7.94e-15 76.6
MsG0480019047.01.T01 MTR_5g084870 23.380 432 270 15 5 413 4 397 1.03e-14 76.3
MsG0480019047.01.T01 MTR_7g014610 25.432 405 249 15 55 421 68 457 2.39e-14 75.1
MsG0480019047.01.T01 MTR_1g040195 24.645 422 260 18 25 427 23 405 2.57e-14 75.1
MsG0480019047.01.T01 MTR_6g053810 25.806 279 184 10 55 323 71 336 2.99e-14 74.7
MsG0480019047.01.T01 MTR_1g040215 23.721 430 260 18 8 408 13 403 8.72e-14 73.6
MsG0480019047.01.T01 MTR_8g079950 25.606 371 235 14 41 396 42 386 1.33e-13 72.8
MsG0480019047.01.T01 MTR_5g090810 24.362 431 262 19 2 392 6 412 2.10e-13 72.4
MsG0480019047.01.T01 MTR_5g090830 24.594 431 261 19 2 392 6 412 8.73e-13 70.5
MsG0480019047.01.T01 MTR_7g086000 26.442 416 254 18 58 439 63 460 1.44e-12 69.7
MsG0480019047.01.T01 MTR_7g014360 26.692 266 175 8 138 395 90 343 1.54e-12 69.3
MsG0480019047.01.T01 MTR_7g085990 26.165 279 187 6 43 308 43 315 2.65e-12 68.9
MsG0480019047.01.T01 MTR_5g090880 24.728 368 229 16 55 393 67 415 5.24e-12 67.8
MsG0480019047.01.T01 MTR_3g450710 26.613 372 220 18 52 392 62 411 1.11e-11 67.0
MsG0480019047.01.T01 MTR_3g450690 26.829 369 220 18 52 392 69 415 1.71e-11 66.2
MsG0480019047.01.T01 MTR_7g013850 21.831 426 263 16 52 427 65 470 1.78e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019047.01.T01 AT5G67150 43.349 436 224 11 16 439 21 445 3.36e-112 337
MsG0480019047.01.T01 AT3G50300 42.069 435 227 11 1 421 1 424 4.39e-100 306
MsG0480019047.01.T01 AT3G50280 40.371 431 239 8 16 437 19 440 2.31e-98 302
MsG0480019047.01.T01 AT5G67160 38.657 432 236 10 17 440 22 432 4.65e-93 288
MsG0480019047.01.T01 AT3G50270 37.969 453 259 10 2 440 4 448 1.12e-91 285
MsG0480019047.01.T01 AT5G42830 37.123 431 246 10 24 440 30 449 1.53e-84 266
MsG0480019047.01.T01 AT5G38130 39.379 419 226 12 16 421 39 442 1.86e-84 266
MsG0480019047.01.T01 AT5G07860 37.701 435 235 10 24 439 31 448 1.91e-80 256
MsG0480019047.01.T01 AT5G07850 37.071 437 241 12 24 440 31 453 7.02e-75 241
MsG0480019047.01.T01 AT5G07870 38.061 423 236 10 38 440 45 461 5.82e-74 239
MsG0480019047.01.T01 AT2G39980 33.473 478 245 13 16 439 21 479 3.40e-68 225
MsG0480019047.01.T01 AT3G50280 38.871 319 180 5 125 437 1 310 1.93e-66 215
MsG0480019047.01.T01 AT5G01210 34.542 469 245 13 16 440 19 469 1.56e-64 215
MsG0480019047.01.T01 AT5G23940 32.751 458 269 13 5 436 4 448 1.74e-64 215
MsG0480019047.01.T01 AT3G50290 40.876 274 148 7 122 389 4 269 6.94e-51 173
MsG0480019047.01.T01 AT5G48930 27.989 368 215 10 53 398 54 393 1.34e-28 117
MsG0480019047.01.T01 AT3G29670 25.117 426 278 14 20 421 25 433 5.60e-25 107
MsG0480019047.01.T01 AT3G48720 27.473 364 229 12 42 392 44 385 9.88e-25 106
MsG0480019047.01.T01 AT3G29590 26.281 449 298 17 3 431 7 442 1.24e-23 103
MsG0480019047.01.T01 AT2G19070 25.054 459 271 21 27 438 19 451 1.06e-22 100
MsG0480019047.01.T01 AT5G41040 28.011 357 218 16 50 392 64 395 3.72e-22 98.6
MsG0480019047.01.T01 AT5G41040 28.011 357 218 16 50 392 80 411 4.37e-22 98.6
MsG0480019047.01.T01 AT5G57840 27.873 409 242 18 41 421 39 422 5.38e-20 92.4
MsG0480019047.01.T01 AT5G39080 25.990 404 245 18 52 421 62 445 7.25e-20 92.0
MsG0480019047.01.T01 AT3G29635 24.771 436 268 16 22 421 27 438 3.46e-19 90.1
MsG0480019047.01.T01 AT5G63560 26.735 389 238 16 50 422 56 413 4.78e-19 89.4
MsG0480019047.01.T01 AT3G29680 25.879 398 248 15 49 421 56 431 6.71e-19 89.0
MsG0480019047.01.T01 AT1G24430 24.654 434 262 19 26 440 38 425 1.47e-18 87.8
MsG0480019047.01.T01 AT5G39050 24.282 383 226 13 66 414 90 442 3.45e-18 87.0
MsG0480019047.01.T01 AT3G26040 24.272 412 257 17 49 440 53 429 5.50e-18 86.3
MsG0480019047.01.T01 AT2G40230 24.645 422 287 16 22 431 24 426 1.20e-17 85.1
MsG0480019047.01.T01 AT1G28680 25.126 398 248 15 58 440 65 427 1.92e-16 81.6
MsG0480019047.01.T01 AT5G07080 27.027 296 183 13 42 321 53 331 5.04e-16 80.5
MsG0480019047.01.T01 AT1G03390 25.490 408 255 19 42 422 59 444 1.55e-15 79.0
MsG0480019047.01.T01 AT5G39090 26.642 274 170 12 135 391 140 399 1.92e-15 78.6
MsG0480019047.01.T01 AT5G61160 21.554 399 267 14 22 392 23 403 8.54e-15 76.6
MsG0480019047.01.T01 AT5G17540 25.792 442 269 21 25 434 23 437 4.15e-14 74.3
MsG0480019047.01.T01 AT5G23970 26.667 450 278 21 4 436 3 417 5.17e-14 73.9
MsG0480019047.01.T01 AT1G28680 24.916 297 202 9 58 348 65 346 5.76e-14 73.6
MsG0480019047.01.T01 AT3G30280 24.000 375 211 17 54 398 61 391 6.71e-14 73.9
MsG0480019047.01.T01 AT5G16410 22.558 430 286 15 1 413 45 444 3.55e-13 71.6
MsG0480019047.01.T01 AT1G65450 25.352 284 188 12 53 324 70 341 7.16e-13 70.5
MsG0480019047.01.T01 AT1G03940 23.020 404 277 11 50 428 61 455 9.10e-13 70.5
MsG0480019047.01.T01 AT1G32910 25.876 371 210 18 53 392 63 399 9.90e-13 70.1
MsG0480019047.01.T01 AT2G23510 24.611 386 255 18 55 422 69 436 1.04e-12 70.1
MsG0480019047.01.T01 AT3G47170 25.839 298 184 11 44 321 64 344 2.44e-12 68.9
MsG0480019047.01.T01 AT2G25150 25.414 362 234 14 50 395 62 403 7.28e-12 67.4
MsG0480019047.01.T01 AT4G15390 22.525 404 258 15 10 394 24 391 2.54e-11 65.9
MsG0480019047.01.T01 AT4G15390 22.525 404 258 15 10 394 24 391 2.54e-11 65.9

Find 82 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4:+13756093 MsG0480019047.01.T01:CDS
GTGTCTTCCTTACAAGATTT+TGG 0.202537 4:+13756224 MsG0480019047.01.T01:CDS
CCATTGCCAGAAGATTATTT+TGG 0.248348 4:+13755862 MsG0480019047.01.T01:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4:+13756105 MsG0480019047.01.T01:CDS
TGGTTGGATAAAGTAGAAAT+TGG 0.327409 4:-13755054 None:intergenic
GCGCTTAATACGTTCATAAT+AGG 0.328958 4:+13755817 MsG0480019047.01.T01:CDS
TGATCATGAATTCATGGATA+TGG 0.332225 4:+13756260 MsG0480019047.01.T01:CDS
AATGTCTTAGCCGTTGAATT+TGG 0.349561 4:-13755117 None:intergenic
TTCTTGTGGATCAAGATGTT+TGG 0.349905 4:-13755791 None:intergenic
AATGAAAGCTGGGGAGTTGT+TGG 0.360707 4:+13755912 MsG0480019047.01.T01:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.360883 4:-13755528 None:intergenic
TTTCATTGCTTTGATTGTAT+TGG 0.364692 4:-13754993 None:intergenic
GGAATGGTTTGTGCCTTTGC+TGG 0.368156 4:+13756171 MsG0480019047.01.T01:CDS
TGAGAAATCCAAGCTTCATT+AGG 0.373854 4:+13756028 MsG0480019047.01.T01:CDS
GCGTTTCCAAAATAATCTTC+TGG 0.379778 4:-13755868 None:intergenic
GCCAGCAAGAGGTTCGAAAA+AGG 0.381613 4:-13755158 None:intergenic
CTAGCTGACGGCATCTTCAT+AGG 0.398680 4:+13755403 MsG0480019047.01.T01:CDS
GGGAGTTGTTGGAGGAAGGT+GGG 0.400037 4:+13755923 MsG0480019047.01.T01:CDS
GAAACGCGATTATAGGTTGC+GGG 0.410719 4:+13755884 MsG0480019047.01.T01:CDS
ATGGGCTGTTGAATACCATT+TGG 0.419783 4:-13755550 None:intergenic
TTGTATTGGTGGAGAGTACT+TGG 0.455345 4:-13754979 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.455624 4:-13756082 None:intergenic
GGGAAATGATCATGAATTCA+TGG 0.457595 4:+13756254 MsG0480019047.01.T01:CDS
TTGTTGGAGGAAGGTGGGAT+TGG 0.457605 4:+13755928 MsG0480019047.01.T01:CDS
GGGGAGTTGTTGGAGGAAGG+TGG 0.459892 4:+13755922 MsG0480019047.01.T01:CDS
CGGAGTGGAAATAGAACAAA+TGG 0.464324 4:+13756150 MsG0480019047.01.T01:CDS
TGCGGGTTTAAAATGAAAGC+TGG 0.465815 4:+13755901 MsG0480019047.01.T01:CDS
GGCAATGGTGGAACAAGTCT+TGG 0.472119 4:-13755847 None:intergenic
CCAAAATAATCTTCTGGCAA+TGG 0.477773 4:-13755862 None:intergenic
GCTGGGGAGTTGTTGGAGGA+AGG 0.483907 4:+13755919 MsG0480019047.01.T01:CDS
AAAACCCACATATGTTCCTT+CGG 0.485243 4:+13755297 MsG0480019047.01.T01:CDS
GAGGAAGGTGGGATTGGTAA+AGG 0.486075 4:+13755934 MsG0480019047.01.T01:CDS
CATTGCTTTGATTGTATTGG+TGG 0.486507 4:-13754990 None:intergenic
CTATCAAGCCTAATGAAGCT+TGG 0.491157 4:-13756036 None:intergenic
GGGGAAAGCCTGTGGCAGTT+CGG 0.491671 4:+13756130 MsG0480019047.01.T01:CDS
ATAAAATTGATTTAACTCCA+TGG 0.501405 4:+13755026 MsG0480019047.01.T01:CDS
ATTGCCTCCAAAATCTTGTA+AGG 0.504306 4:-13756231 None:intergenic
TAAAATTGATTTAACTCCAT+GGG 0.505681 4:+13755027 MsG0480019047.01.T01:CDS
GGAAACCAACGCTCTAGTGT+TGG 0.511281 4:-13755529 None:intergenic
GCCTTTGCTGGAATAGAAGA+AGG 0.511653 4:+13756183 MsG0480019047.01.T01:CDS
TTCAATCATGTTGTAGCTGA+CGG 0.511810 4:+13755433 MsG0480019047.01.T01:CDS
TATAGTTAAAGGAAATCGTA+TGG 0.515144 4:-13755569 None:intergenic
CTAGAGCGTTGGTTTCCAAA+TGG 0.515960 4:+13755535 MsG0480019047.01.T01:CDS
TTTCCACTCCGAACTGCCAC+AGG 0.522499 4:-13756138 None:intergenic
TGAATTTGGTCTGATGCTAC+AGG 0.526430 4:-13755103 None:intergenic
TGTAAGGAAGACACACTTGA+AGG 0.538009 4:-13756215 None:intergenic
TAAAGAATGATTATGAGTCA+TGG 0.538860 4:+13756004 MsG0480019047.01.T01:CDS
GCGGGTTTAAAATGAAAGCT+GGG 0.538973 4:+13755902 MsG0480019047.01.T01:CDS
GTATTAAGCGCATCTTCTTG+TGG 0.540410 4:-13755805 None:intergenic
AGGTACATCAAAATCATTGT+TGG 0.540953 4:+13755363 MsG0480019047.01.T01:CDS
GAAAGCTGGGGAGTTGTTGG+AGG 0.541663 4:+13755915 MsG0480019047.01.T01:CDS
TGGCCATTAGCAGTTCTCCA+TGG 0.541677 4:+13756079 MsG0480019047.01.T01:CDS
AAATAATCTTCTGGCAATGG+TGG 0.542517 4:-13755859 None:intergenic
GGAAACGCGATTATAGGTTG+CGG 0.544739 4:+13755883 MsG0480019047.01.T01:CDS
TAGTTTGTAACTCCGTTGAA+AGG 0.548129 4:-13755337 None:intergenic
CGTGTTCTGTAACTTTGAGA+CGG 0.548497 4:-13755180 None:intergenic
TGGAAATAGAACAAATGGAA+TGG 0.553380 4:+13756155 MsG0480019047.01.T01:CDS
ACCTTCTTCTATTCCAGCAA+AGG 0.555908 4:-13756184 None:intergenic
AGTAGAAATTGGAGATCCCA+TGG 0.565431 4:-13755043 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.569521 4:-13755527 None:intergenic
ATGGCATTTCATCAATTCGT+GGG 0.570140 4:+13755465 MsG0480019047.01.T01:CDS
AACAACCGAAGGAACATATG+TGG 0.575430 4:-13755302 None:intergenic
ATAGTTAAAGGAAATCGTAT+GGG 0.585010 4:-13755568 None:intergenic
CTACAGGATGGTGATAAAGA+AGG 0.592406 4:-13755087 None:intergenic
CATGGCATTTCATCAATTCG+TGG 0.610417 4:+13755464 MsG0480019047.01.T01:CDS
ACTTTGAGACGGCCAGCAAG+AGG 0.616523 4:-13755169 None:intergenic
TCTGTAACATGCAATAATGC+AGG 0.619827 4:+13755223 MsG0480019047.01.T01:CDS
ATTGTTGGCAGTTCAAGTGA+CGG 0.619862 4:+13755378 MsG0480019047.01.T01:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.632370 4:-13755526 None:intergenic
TAGCTGACGGACAGTCCACA+TGG 0.634434 4:+13755446 MsG0480019047.01.T01:CDS
CAAGTGACGGAGCTAGCTGA+CGG 0.639821 4:+13755391 MsG0480019047.01.T01:CDS
CGGGTTTAAAATGAAAGCTG+GGG 0.642028 4:+13755903 MsG0480019047.01.T01:CDS
AAGCCTGTGGCAGTTCGGAG+TGG 0.647498 4:+13756135 MsG0480019047.01.T01:CDS
ACAACCGAAGGAACATATGT+GGG 0.650889 4:-13755301 None:intergenic
AACGGAGTTACAAACTACGA+AGG 0.656439 4:+13755343 MsG0480019047.01.T01:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4:-13756096 None:intergenic
TAGCATCAGACCAAATTCAA+CGG 0.663707 4:+13755107 MsG0480019047.01.T01:CDS
AGGTGTAAACTTTATACATG+CGG 0.677512 4:+13755243 MsG0480019047.01.T01:CDS
GCCACAAAAGAATTACTCTG+AGG 0.711684 4:+13755594 MsG0480019047.01.T01:CDS
CGAATTGATGAAATGCCATG+TGG 0.718082 4:-13755461 None:intergenic
TTTGGTCTGATGCTACAGGA+TGG 0.756053 4:-13755099 None:intergenic
TCGTGGGCTGAAATGTCACG+TGG 0.758657 4:+13755481 MsG0480019047.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAATTGATTTAACTCCA+TGG + Chr4:13755026-13755045 MsG0480019047.01.T01:CDS 20.0%
!!! TAAAATTGATTTAACTCCAT+GGG + Chr4:13755027-13755046 MsG0480019047.01.T01:CDS 20.0%
!!! TTGTAGAGAAGATATTTTTT+TGG - Chr4:13755734-13755753 None:intergenic 20.0%
! AAAAATTCTATCTATCTGAG+AGG + Chr4:13755644-13755663 MsG0480019047.01.T01:CDS 25.0%
! ATAGTTAAAGGAAATCGTAT+GGG - Chr4:13755571-13755590 None:intergenic 25.0%
! TAAAGAATGATTATGAGTCA+TGG + Chr4:13756004-13756023 MsG0480019047.01.T01:CDS 25.0%
! TATAGTTAAAGGAAATCGTA+TGG - Chr4:13755572-13755591 None:intergenic 25.0%
! TTTCATTGCTTTGATTGTAT+TGG - Chr4:13754996-13755015 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + Chr4:13756105-13756124 MsG0480019047.01.T01:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + Chr4:13756110-13756129 MsG0480019047.01.T01:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + Chr4:13756109-13756128 MsG0480019047.01.T01:CDS 25.0%
AGGTACATCAAAATCATTGT+TGG + Chr4:13755363-13755382 MsG0480019047.01.T01:CDS 30.0%
AGGTGTAAACTTTATACATG+CGG + Chr4:13755243-13755262 MsG0480019047.01.T01:CDS 30.0%
CTTTATCCAACCAACAAAAA+AGG + Chr4:13755064-13755083 MsG0480019047.01.T01:CDS 30.0%
TGATCATGAATTCATGGATA+TGG + Chr4:13756260-13756279 MsG0480019047.01.T01:CDS 30.0%
TGGAAATAGAACAAATGGAA+TGG + Chr4:13756155-13756174 MsG0480019047.01.T01:CDS 30.0%
TGGTTGGATAAAGTAGAAAT+TGG - Chr4:13755057-13755076 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG - Chr4:13756099-13756118 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG + Chr4:13756111-13756130 MsG0480019047.01.T01:CDS 30.0%
!! TATTTTGGAAACGCGATTAT+AGG + Chr4:13755877-13755896 MsG0480019047.01.T01:CDS 30.0%
!! TTTTGTGGCTCTATAGTTAA+AGG - Chr4:13755583-13755602 None:intergenic 30.0%
!!! ATAAAGAAGGCCTTTTTTGT+TGG - Chr4:13755077-13755096 None:intergenic 30.0%
!!! CGTTCATTTTTTCCTTTCAA+CGG + Chr4:13755325-13755344 MsG0480019047.01.T01:CDS 30.0%
!!! CTTGAGCACTAAGAATTTTT+TGG + Chr4:13756059-13756078 MsG0480019047.01.T01:CDS 30.0%
AAAAATGAACGAACAACCGA+AGG - Chr4:13755316-13755335 None:intergenic 35.0%
AAAACCCACATATGTTCCTT+CGG + Chr4:13755297-13755316 MsG0480019047.01.T01:CDS 35.0%
AAATAATCTTCTGGCAATGG+TGG - Chr4:13755862-13755881 None:intergenic 35.0%
AAGAGGTTCGAAAAAGGAAA+GGG - Chr4:13755155-13755174 None:intergenic 35.0%
AATGTCTTAGCCGTTGAATT+TGG - Chr4:13755120-13755139 None:intergenic 35.0%
ATGGCATTTCATCAATTCGT+GGG + Chr4:13755465-13755484 MsG0480019047.01.T01:CDS 35.0%
ATTGCCTCCAAAATCTTGTA+AGG - Chr4:13756234-13756253 None:intergenic 35.0%
CATTGCTTTGATTGTATTGG+TGG - Chr4:13754993-13755012 None:intergenic 35.0%
CCAAAATAATCTTCTGGCAA+TGG - Chr4:13755865-13755884 None:intergenic 35.0%
GCGCTTAATACGTTCATAAT+AGG + Chr4:13755817-13755836 MsG0480019047.01.T01:CDS 35.0%
GCGTTTCCAAAATAATCTTC+TGG - Chr4:13755871-13755890 None:intergenic 35.0%
GGGAAATGATCATGAATTCA+TGG + Chr4:13756254-13756273 MsG0480019047.01.T01:CDS 35.0%
TAGCATCAGACCAAATTCAA+CGG + Chr4:13755107-13755126 MsG0480019047.01.T01:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG + Chr4:13756093-13756112 MsG0480019047.01.T01:CDS 35.0%
TCTGTAACATGCAATAATGC+AGG + Chr4:13755223-13755242 MsG0480019047.01.T01:CDS 35.0%
TGAGAAATCCAAGCTTCATT+AGG + Chr4:13756028-13756047 MsG0480019047.01.T01:CDS 35.0%
TTCAATCATGTTGTAGCTGA+CGG + Chr4:13755433-13755452 MsG0480019047.01.T01:CDS 35.0%
TTCTTGTGGATCAAGATGTT+TGG - Chr4:13755794-13755813 None:intergenic 35.0%
! AAGGAACATATGTGGGTTTT+AGG - Chr4:13755297-13755316 None:intergenic 35.0%
! CCATTGCCAGAAGATTATTT+TGG + Chr4:13755862-13755881 MsG0480019047.01.T01:CDS 35.0%
! GTGTCTTCCTTACAAGATTT+TGG + Chr4:13756224-13756243 MsG0480019047.01.T01:CDS 35.0%
! TAGTTTGTAACTCCGTTGAA+AGG - Chr4:13755340-13755359 None:intergenic 35.0%
! TCCTCAGAGTAATTCTTTTG+TGG - Chr4:13755598-13755617 None:intergenic 35.0%
! TCTTCCTTACAAGATTTTGG+AGG + Chr4:13756227-13756246 MsG0480019047.01.T01:CDS 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG + Chr4:13755752-13755771 MsG0480019047.01.T01:CDS 35.0%
!! TACAAGATTTTGGAGGCAAT+GGG + Chr4:13756234-13756253 MsG0480019047.01.T01:CDS 35.0%
!! TTACAAGATTTTGGAGGCAA+TGG + Chr4:13756233-13756252 MsG0480019047.01.T01:CDS 35.0%
AAACCATGGAGAACTGCTAA+TGG - Chr4:13756085-13756104 None:intergenic 40.0%
AACAACCGAAGGAACATATG+TGG - Chr4:13755305-13755324 None:intergenic 40.0%
AACGGAGTTACAAACTACGA+AGG + Chr4:13755343-13755362 MsG0480019047.01.T01:CDS 40.0%
ACAACCGAAGGAACATATGT+GGG - Chr4:13755304-13755323 None:intergenic 40.0%
ACCTTCTTCTATTCCAGCAA+AGG - Chr4:13756187-13756206 None:intergenic 40.0%
AGTAGAAATTGGAGATCCCA+TGG - Chr4:13755046-13755065 None:intergenic 40.0%
ATTGTTGGCAGTTCAAGTGA+CGG + Chr4:13755378-13755397 MsG0480019047.01.T01:CDS 40.0%
CAAGAGGTTCGAAAAAGGAA+AGG - Chr4:13755156-13755175 None:intergenic 40.0%
CATGGCATTTCATCAATTCG+TGG + Chr4:13755464-13755483 MsG0480019047.01.T01:CDS 40.0%
CGAATTGATGAAATGCCATG+TGG - Chr4:13755464-13755483 None:intergenic 40.0%
CGGAGTGGAAATAGAACAAA+TGG + Chr4:13756150-13756169 MsG0480019047.01.T01:CDS 40.0%
CTACAGGATGGTGATAAAGA+AGG - Chr4:13755090-13755109 None:intergenic 40.0%
CTATCAAGCCTAATGAAGCT+TGG - Chr4:13756039-13756058 None:intergenic 40.0%
GCCACAAAAGAATTACTCTG+AGG + Chr4:13755594-13755613 MsG0480019047.01.T01:CDS 40.0%
GTATTAAGCGCATCTTCTTG+TGG - Chr4:13755808-13755827 None:intergenic 40.0%
TGAATTTGGTCTGATGCTAC+AGG - Chr4:13755106-13755125 None:intergenic 40.0%
TGTAAGGAAGACACACTTGA+AGG - Chr4:13756218-13756237 None:intergenic 40.0%
! ATGGGCTGTTGAATACCATT+TGG - Chr4:13755553-13755572 None:intergenic 40.0%
! CGGGTTTAAAATGAAAGCTG+GGG + Chr4:13755903-13755922 MsG0480019047.01.T01:CDS 40.0%
! CGTGTTCTGTAACTTTGAGA+CGG - Chr4:13755183-13755202 None:intergenic 40.0%
! GCGGGTTTAAAATGAAAGCT+GGG + Chr4:13755902-13755921 MsG0480019047.01.T01:CDS 40.0%
! TGCGGGTTTAAAATGAAAGC+TGG + Chr4:13755901-13755920 MsG0480019047.01.T01:CDS 40.0%
!! TTGTATTGGTGGAGAGTACT+TGG - Chr4:13754982-13755001 None:intergenic 40.0%
!!! AGAAGGCCTTTTTTGTTGGT+TGG - Chr4:13755073-13755092 None:intergenic 40.0%
AAACCAACGCTCTAGTGTTG+GGG - Chr4:13755530-13755549 None:intergenic 45.0%
AATGAAAGCTGGGGAGTTGT+TGG + Chr4:13755912-13755931 MsG0480019047.01.T01:CDS 45.0%
GAAACCAACGCTCTAGTGTT+GGG - Chr4:13755531-13755550 None:intergenic 45.0%
GAAACGCGATTATAGGTTGC+GGG + Chr4:13755884-13755903 MsG0480019047.01.T01:CDS 45.0%
GCCTTTGCTGGAATAGAAGA+AGG + Chr4:13756183-13756202 MsG0480019047.01.T01:CDS 45.0%
GGAAACGCGATTATAGGTTG+CGG + Chr4:13755883-13755902 MsG0480019047.01.T01:CDS 45.0%
TTTGGTCTGATGCTACAGGA+TGG - Chr4:13755102-13755121 None:intergenic 45.0%
!! CTAGAGCGTTGGTTTCCAAA+TGG + Chr4:13755535-13755554 MsG0480019047.01.T01:CDS 45.0%
!!! TCCTTTTTCGAACCTCTTGC+TGG + Chr4:13755157-13755176 MsG0480019047.01.T01:CDS 45.0%
CTAGCTGACGGCATCTTCAT+AGG + Chr4:13755403-13755422 MsG0480019047.01.T01:CDS 50.0%
GCCAGCAAGAGGTTCGAAAA+AGG - Chr4:13755161-13755180 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG - Chr4:13755532-13755551 None:intergenic 50.0%
TGGCCATTAGCAGTTCTCCA+TGG + Chr4:13756079-13756098 MsG0480019047.01.T01:CDS 50.0%
TTGTTGGAGGAAGGTGGGAT+TGG + Chr4:13755928-13755947 MsG0480019047.01.T01:CDS 50.0%
! AACCAACGCTCTAGTGTTGG+GGG - Chr4:13755529-13755548 None:intergenic 50.0%
! GAGGAAGGTGGGATTGGTAA+AGG + Chr4:13755934-13755953 MsG0480019047.01.T01:CDS 50.0%
! GGAATGGTTTGTGCCTTTGC+TGG + Chr4:13756171-13756190 MsG0480019047.01.T01:CDS 50.0%
! GGCAATGGTGGAACAAGTCT+TGG - Chr4:13755850-13755869 None:intergenic 50.0%
! TTTTGGATGGGGAAAGCCTG+TGG + Chr4:13756122-13756141 MsG0480019047.01.T01:CDS 50.0%
AACCCCCAACACTAGAGCGT+TGG + Chr4:13755524-13755543 MsG0480019047.01.T01:CDS 55.0%
GAAAGCTGGGGAGTTGTTGG+AGG + Chr4:13755915-13755934 MsG0480019047.01.T01:CDS 55.0%
GGGAGTTGTTGGAGGAAGGT+GGG + Chr4:13755923-13755942 MsG0480019047.01.T01:CDS 55.0%
TTTCCACTCCGAACTGCCAC+AGG - Chr4:13756141-13756160 None:intergenic 55.0%
! CAAGTGACGGAGCTAGCTGA+CGG + Chr4:13755391-13755410 MsG0480019047.01.T01:CDS 55.0%
! TAGCTGACGGACAGTCCACA+TGG + Chr4:13755446-13755465 MsG0480019047.01.T01:CDS 55.0%
! TCGTGGGCTGAAATGTCACG+TGG + Chr4:13755481-13755500 MsG0480019047.01.T01:CDS 55.0%
!! ACTTTGAGACGGCCAGCAAG+AGG - Chr4:13755172-13755191 None:intergenic 55.0%
AAGCCTGTGGCAGTTCGGAG+TGG + Chr4:13756135-13756154 MsG0480019047.01.T01:CDS 60.0%
GCTGGGGAGTTGTTGGAGGA+AGG + Chr4:13755919-13755938 MsG0480019047.01.T01:CDS 60.0%
GGGGAAAGCCTGTGGCAGTT+CGG + Chr4:13756130-13756149 MsG0480019047.01.T01:CDS 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG + Chr4:13755922-13755941 MsG0480019047.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 13754968 13756290 13754968 ID=MsG0480019047.01;Name=MsG0480019047.01
Chr4 mRNA 13754968 13756290 13754968 ID=MsG0480019047.01.T01;Parent=MsG0480019047.01;Name=MsG0480019047.01.T01;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|440
Chr4 exon 13754968 13756290 13754968 ID=MsG0480019047.01.T01:exon:34130;Parent=MsG0480019047.01.T01
Chr4 CDS 13754968 13756290 13754968 ID=MsG0480019047.01.T01:cds;Parent=MsG0480019047.01.T01
Gene Sequence

>MsG0480019047.01.T01

ATGGGTGCATTCCAAGTACTCTCCACCAATACAATCAAAGCAATGAAATCAAGTAATCATAAAATTGATTTAACTCCATGGGATCTCCAATTTCTACTTTATCCAACCAACAAAAAAGGCCTTCTTTATCACCATCCTGTAGCATCAGACCAAATTCAACGGCTAAGACATTCTCTTTCTTCTGCCCTTTCCTTTTTCGAACCTCTTGCTGGCCGTCTCAAAGTTACAGAACACGAAGACAGTACTGTCTCGTGTTCTGTAACATGCAATAATGCAGGTGTAAACTTTATACATGCGGCAGCAAAGAATACTTGTGTTGCTGACATCCTAAAACCCACATATGTTCCTTCGGTTGTTCGTTCATTTTTTCCTTTCAACGGAGTTACAAACTACGAAGGTACATCAAAATCATTGTTGGCAGTTCAAGTGACGGAGCTAGCTGACGGCATCTTCATAGGCTGTAATTTCAATCATGTTGTAGCTGACGGACAGTCCACATGGCATTTCATCAATTCGTGGGCTGAAATGTCACGTGGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCAAATGGTATTCAACAGCCCATACGATTTCCTTTAACTATAGAGCCACAAAAGAATTACTCTGAGGAGTTGAGTTTTTCGTCATCTGATGACGAAAAATTCTATCTATCTGAGAGGATATTTCACTTCACAAAGAAGAAAATCATGCAATTGAAATCAAAAGTCAACGCAGCGATTGATACCAAAAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGTTCAGTGATTCGTTCCAAACATCTTGATCCACAAGAAGATGCGCTTAATACGTTCATAATAGGAGTTAGACCAAGACTTGTTCCACCATTGCCAGAAGATTATTTTGGAAACGCGATTATAGGTTGCGGGTTTAAAATGAAAGCTGGGGAGTTGTTGGAGGAAGGTGGGATTGGTAAAGGTGCTTTGTTGATGAATAAGTTAATTGCTTTGCACTCTAATGAGAAATTAAAGAATGATTATGAGTCATGGTTGAGAAATCCAAGCTTCATTAGGCTTGATAGCTTGAGCACTAAGAATTTTTTGGCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTGGCAGTTCGGAGTGGAAATAGAACAAATGGAATGGTTTGTGCCTTTGCTGGAATAGAAGAAGGTAGCATTGACCTTCAAGTGTGTCTTCCTTACAAGATTTTGGAGGCAATGGGAAATGATCATGAATTCATGGATATGGTTAACTAA
Protein sequence

>MsG0480019047.01.T01

MGAFQVLSTNTIKAMKSSNHKIDLTPWDLQFLLYPTNKKGLLYHHPVASDQIQRLRHSLSSALSFFEPLAGRLKVTEHEDSTVSCSVTCNNAGVNFIHAAAKNTCVADILKPTYVPSVVRSFFPFNGVTNYEGTSKSLLAVQVTELADGIFIGCNFNHVVADGQSTWHFINSWAEMSRGCHHQISKPPTLERWFPNGIQQPIRFPLTIEPQKNYSEELSFSSSDDEKFYLSERIFHFTKKKIMQLKSKVNAAIDTKKISSLQALLTHLWCSVIRSKHLDPQEDALNTFIIGVRPRLVPPLPEDYFGNAIIGCGFKMKAGELLEEGGIGKGALLMNKLIALHSNEKLKNDYESWLRNPSFIRLDSLSTKNFLAISSSPWFDVYGNDFGWGKPVAVRSGNRTNGMVCAFAGIEEGSIDLQVCLPYKILEAMGNDHEFMDMVN*