AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017154.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017154.01.T01 MTR_3g102020 95.261 422 20 0 1 422 36 457 0.0 838
MsG0380017154.01.T01 MTR_3g101960 90.024 411 40 1 1 411 36 445 0.0 781
MsG0380017154.01.T01 MTR_3g102010 86.150 426 50 3 1 422 37 457 0.0 760
MsG0380017154.01.T01 MTR_3g101970 85.132 417 48 1 1 417 36 438 0.0 748
MsG0380017154.01.T01 MTR_3g101900 69.856 418 120 3 1 417 37 449 0.0 596
MsG0380017154.01.T01 MTR_3g101930 67.064 419 133 3 1 415 37 454 0.0 583
MsG0380017154.01.T01 MTR_3g102030 79.245 318 40 2 52 369 29 320 0.0 522
MsG0380017154.01.T01 MTR_3g070170 41.009 456 196 12 1 412 40 466 1.85e-102 313
MsG0380017154.01.T01 MTR_4g015180 40.213 470 211 11 1 416 34 487 4.82e-97 299
MsG0380017154.01.T01 MTR_5g081960 39.186 467 211 11 1 412 34 482 2.65e-95 295
MsG0380017154.01.T01 MTR_8g041770 40.291 412 229 9 8 416 44 441 6.19e-92 285
MsG0380017154.01.T01 MTR_4g022825 39.048 420 228 10 8 416 38 440 1.59e-86 270
MsG0380017154.01.T01 MTR_4g022800 38.389 422 230 11 8 416 38 442 3.74e-85 267
MsG0380017154.01.T01 MTR_4g022720 39.952 413 227 9 9 416 39 435 5.25e-85 266
MsG0380017154.01.T01 MTR_4g094842 39.379 419 225 11 8 412 41 444 1.36e-84 266
MsG0380017154.01.T01 MTR_3g088420 39.277 415 227 10 8 416 38 433 3.50e-84 264
MsG0380017154.01.T01 MTR_4g094835 38.741 413 231 8 7 412 45 442 8.02e-84 264
MsG0380017154.01.T01 MTR_0190s0020 38.257 413 238 8 8 416 43 442 2.63e-83 262
MsG0380017154.01.T01 MTR_4g094838 38.554 415 219 8 7 412 41 428 2.98e-83 262
MsG0380017154.01.T01 MTR_4g022670 38.571 420 231 10 8 416 38 441 4.89e-81 256
MsG0380017154.01.T01 MTR_7g026860 37.381 420 233 11 8 416 38 438 1.50e-78 250
MsG0380017154.01.T01 MTR_3g026310 37.939 427 235 12 11 416 31 448 7.26e-78 248
MsG0380017154.01.T01 MTR_4g094828 38.318 428 229 14 7 417 42 451 4.38e-77 246
MsG0380017154.01.T01 MTR_4g022290 36.816 402 221 11 7 395 43 424 3.45e-73 236
MsG0380017154.01.T01 MTR_4g094832 41.834 349 188 8 71 412 86 426 3.39e-72 233
MsG0380017154.01.T01 MTR_8g079710 38.916 406 212 17 7 397 42 426 4.44e-70 228
MsG0380017154.01.T01 MTR_4g022760 36.141 368 199 11 8 352 38 392 5.91e-62 206
MsG0380017154.01.T01 MTR_6g045553 32.812 448 251 11 2 415 43 474 7.96e-58 197
MsG0380017154.01.T01 MTR_6g016815 31.951 410 202 12 11 416 45 381 1.15e-46 165
MsG0380017154.01.T01 MTR_8g040780 36.607 224 137 4 200 419 14 236 1.30e-39 142
MsG0380017154.01.T01 MTR_8g075610 27.157 394 240 14 34 406 47 414 1.02e-32 128
MsG0380017154.01.T01 MTR_2g064495 26.753 385 256 13 34 406 56 426 7.60e-32 125
MsG0380017154.01.T01 MTR_8g096530 31.013 316 178 11 28 324 53 347 2.29e-31 124
MsG0380017154.01.T01 MTR_4g007540 26.650 394 246 13 34 406 56 427 4.19e-31 124
MsG0380017154.01.T01 MTR_2g105330 26.494 385 257 13 34 406 56 426 4.75e-31 124
MsG0380017154.01.T01 MTR_7g026850 41.026 156 80 4 8 162 21 165 9.50e-30 114
MsG0380017154.01.T01 MTR_3g098980 29.703 303 186 9 34 324 66 353 7.84e-29 117
MsG0380017154.01.T01 MTR_3g049150 27.594 424 239 17 22 406 46 440 8.41e-29 117
MsG0380017154.01.T01 MTR_4g127650 26.988 415 230 16 30 402 62 445 1.56e-27 114
MsG0380017154.01.T01 MTR_7g026770 37.288 177 90 3 121 297 132 287 5.99e-27 109
MsG0380017154.01.T01 MTR_3g026950 25.000 424 253 16 28 411 48 446 2.01e-26 110
MsG0380017154.01.T01 MTR_4g097090 26.961 408 240 14 30 402 61 445 7.63e-26 109
MsG0380017154.01.T01 MTR_8g040760 36.612 183 104 5 8 189 41 212 1.02e-24 101
MsG0380017154.01.T01 MTR_4g094825 38.000 150 82 2 267 416 253 391 2.64e-24 103
MsG0380017154.01.T01 MTR_4g094825 30.396 227 127 7 7 217 105 316 1.04e-19 90.9
MsG0380017154.01.T01 MTR_7g027175 29.841 315 195 10 22 324 56 356 1.38e-23 102
MsG0380017154.01.T01 MTR_6g034495 43.220 118 63 3 45 159 45 161 3.43e-23 95.9
MsG0380017154.01.T01 MTR_6g053810 31.818 286 177 12 35 310 72 349 3.48e-23 101
MsG0380017154.01.T01 MTR_3g112180 27.042 355 217 13 31 365 58 390 1.65e-22 99.8
MsG0380017154.01.T01 MTR_5g076700 25.296 423 274 17 1 402 30 431 3.15e-22 99.0
MsG0380017154.01.T01 MTR_7g114470 25.714 420 277 16 1 402 31 433 1.42e-21 97.1
MsG0380017154.01.T01 MTR_3g071320 28.710 310 190 10 33 324 59 355 5.29e-21 95.1
MsG0380017154.01.T01 MTR_7g114550 26.196 439 275 20 1 414 30 444 1.88e-20 93.6
MsG0380017154.01.T01 MTR_1g081660 25.185 405 265 14 31 419 70 452 3.18e-20 92.8
MsG0380017154.01.T01 MTR_1g040195 23.709 426 282 16 13 416 16 420 6.29e-20 91.7
MsG0380017154.01.T01 MTR_1g040175 27.248 367 230 17 31 385 61 402 6.64e-20 92.0
MsG0380017154.01.T01 MTR_3g465980 26.712 292 196 8 18 300 52 334 1.93e-19 90.5
MsG0380017154.01.T01 MTR_4g055170 26.690 281 179 8 34 300 69 336 4.94e-19 89.4
MsG0380017154.01.T01 MTR_7g086000 26.768 396 238 19 37 397 63 441 1.20e-18 88.2
MsG0380017154.01.T01 MTR_7g114560 25.459 436 282 19 1 414 30 444 1.10e-17 85.1
MsG0380017154.01.T01 MTR_3g465930 28.058 278 178 8 35 300 70 337 1.60e-17 84.7
MsG0380017154.01.T01 MTR_3g465990 26.621 293 195 9 18 300 52 334 1.68e-17 84.7
MsG0380017154.01.T01 MTR_7g114960 25.815 399 265 11 31 412 60 444 2.55e-17 84.0
MsG0380017154.01.T01 MTR_3g465950 28.302 265 169 7 35 287 69 324 4.79e-17 83.2
MsG0380017154.01.T01 MTR_2g015430 25.926 297 200 9 37 324 61 346 6.38e-17 82.8
MsG0380017154.01.T01 MTR_1g040215 27.835 291 190 11 31 313 61 339 9.29e-17 82.4
MsG0380017154.01.T01 MTR_6g015850 25.420 417 249 15 26 407 62 451 1.16e-16 82.0
MsG0380017154.01.T01 MTR_6g034485 35.043 117 69 2 171 283 2 115 1.21e-16 76.3
MsG0380017154.01.T01 MTR_5g026320 24.586 423 262 17 13 411 59 448 3.10e-16 80.9
MsG0380017154.01.T01 MTR_3g465910 28.674 279 167 9 35 297 69 331 6.34e-16 80.1
MsG0380017154.01.T01 MTR_7g084940 24.038 312 206 10 30 326 65 360 9.42e-16 79.3
MsG0380017154.01.T01 MTR_4g013050 23.691 401 232 16 32 406 51 403 1.14e-15 79.0
MsG0380017154.01.T01 MTR_6g015830 24.882 422 248 16 26 407 62 454 2.03e-15 78.2
MsG0380017154.01.T01 MTR_7g026830 50.000 68 33 1 77 143 28 95 2.16e-14 68.9
MsG0380017154.01.T01 MTR_7g014160 24.777 448 267 18 7 407 43 467 5.73e-14 73.9
MsG0380017154.01.T01 MTR_3g049140 25.980 204 133 5 208 397 27 226 6.37e-14 71.6
MsG0380017154.01.T01 MTR_7g085990 23.358 411 260 14 31 402 52 446 4.04e-13 71.2
MsG0380017154.01.T01 MTR_6g015320 24.138 406 259 15 26 402 58 443 4.28e-13 71.2
MsG0380017154.01.T01 MTR_3g450710 23.473 311 175 11 28 302 59 342 4.71e-13 70.9
MsG0380017154.01.T01 MTR_1g040315 26.246 301 196 11 1 291 29 313 2.04e-12 68.9
MsG0380017154.01.T01 MTR_7g013850 23.358 411 250 15 7 368 42 436 2.14e-12 68.9
MsG0380017154.01.T01 MTR_7g014620 24.130 431 275 21 2 407 23 426 3.69e-12 68.2
MsG0380017154.01.T01 MTR_7g014200 23.843 432 286 19 7 407 43 462 5.86e-12 67.8
MsG0380017154.01.T01 MTR_6g015315 24.574 411 266 19 19 402 48 441 1.56e-11 66.2
MsG0380017154.01.T01 MTR_5g090830 23.779 307 187 15 29 308 61 347 4.77e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017154.01.T01 AT5G42830 57.619 420 169 2 1 417 37 450 2.27e-180 510
MsG0380017154.01.T01 AT5G07850 55.635 417 183 2 1 416 38 453 4.72e-173 492
MsG0380017154.01.T01 AT5G07860 55.502 418 176 5 1 415 38 448 1.21e-162 466
MsG0380017154.01.T01 AT5G07870 52.336 428 188 3 1 416 38 461 2.71e-159 457
MsG0380017154.01.T01 AT2G39980 40.215 465 209 9 1 416 36 480 6.64e-104 317
MsG0380017154.01.T01 AT5G67150 40.566 424 231 10 2 417 37 447 4.79e-96 295
MsG0380017154.01.T01 AT5G01210 39.868 454 217 10 1 416 34 469 9.44e-91 283
MsG0380017154.01.T01 AT3G50270 35.897 429 248 12 1 417 36 449 3.27e-77 247
MsG0380017154.01.T01 AT3G50280 36.190 420 246 11 1 412 34 439 4.71e-73 236
MsG0380017154.01.T01 AT3G50300 33.645 428 256 9 1 415 34 446 1.12e-70 230
MsG0380017154.01.T01 AT5G38130 36.029 408 235 12 1 399 54 444 5.15e-70 228
MsG0380017154.01.T01 AT5G67160 34.856 416 244 11 6 417 41 433 2.67e-67 221
MsG0380017154.01.T01 AT5G23940 34.499 429 254 10 2 414 33 450 7.57e-61 205
MsG0380017154.01.T01 AT3G50280 37.459 307 181 6 112 412 8 309 3.62e-55 185
MsG0380017154.01.T01 AT3G50290 37.452 259 149 6 113 362 15 269 9.93e-44 154
MsG0380017154.01.T01 AT5G48930 27.470 415 252 15 13 406 41 427 2.26e-33 130
MsG0380017154.01.T01 AT2G19070 29.314 423 243 17 12 402 42 440 1.27e-32 128
MsG0380017154.01.T01 AT5G41040 29.526 359 206 15 28 365 63 395 1.58e-32 128
MsG0380017154.01.T01 AT5G41040 30.447 358 204 16 28 365 79 411 2.01e-32 128
MsG0380017154.01.T01 AT3G48720 26.598 391 242 15 7 386 40 396 1.68e-23 102
MsG0380017154.01.T01 AT2G40230 27.632 380 206 14 29 375 54 397 5.39e-23 100
MsG0380017154.01.T01 AT5G63560 28.846 312 186 11 28 324 55 345 1.51e-22 99.4
MsG0380017154.01.T01 AT1G65450 26.935 323 201 12 22 324 60 367 2.17e-21 96.3
MsG0380017154.01.T01 AT1G27620 26.984 315 182 10 34 324 58 348 2.14e-20 93.2
MsG0380017154.01.T01 AT5G57840 26.190 420 251 16 11 402 39 427 2.55e-20 93.2
MsG0380017154.01.T01 AT1G28680 26.014 296 200 11 37 324 65 349 3.91e-19 89.7
MsG0380017154.01.T01 AT1G28680 26.014 296 200 11 37 324 65 349 4.02e-19 89.0
MsG0380017154.01.T01 AT2G25150 27.333 300 186 11 27 310 60 343 1.69e-15 78.6
MsG0380017154.01.T01 AT1G78990 24.430 307 196 11 13 308 52 333 1.81e-15 78.6
MsG0380017154.01.T01 AT1G03390 25.248 404 253 18 19 398 64 442 1.90e-15 78.6
MsG0380017154.01.T01 AT5G16410 23.168 423 255 15 32 421 89 474 4.58e-15 77.4
MsG0380017154.01.T01 AT5G07080 25.090 279 173 11 35 295 71 331 6.21e-14 73.9
MsG0380017154.01.T01 AT1G24420 25.191 393 246 17 45 422 76 435 7.42e-14 73.6
MsG0380017154.01.T01 AT1G24420 25.191 393 246 17 45 422 76 435 7.42e-14 73.6
MsG0380017154.01.T01 AT1G65450 25.837 209 133 6 132 324 1 203 1.23e-13 71.6
MsG0380017154.01.T01 AT2G23510 26.667 255 171 7 34 282 69 313 9.45e-12 67.0
MsG0380017154.01.T01 AT1G03940 22.249 418 280 18 22 407 54 458 1.46e-11 66.6
MsG0380017154.01.T01 AT5G23970 24.566 403 247 17 31 412 51 417 6.61e-11 64.3
MsG0380017154.01.T01 AT3G29590 22.997 387 256 14 41 402 68 437 8.41e-11 63.9

Find 95 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTTCCTAAGGAATATTT+TGG 0.126132 3:-92705300 MsG0380017154.01.T01:CDS
CATTCAAGTTACTGAATTAT+TGG 0.137906 3:-92705757 MsG0380017154.01.T01:CDS
AATATGTATGGGAATGAATT+TGG 0.211414 3:-92705060 MsG0380017154.01.T01:CDS
AATATTCTCTCTCTCAAAAT+AGG 0.288986 3:+92705513 None:intergenic
AGGAAGAGCTGGTTCCATTC+TGG 0.290483 3:+92705314 None:intergenic
AATCTTTATCAGCACTAGTT+TGG 0.302845 3:-92705410 MsG0380017154.01.T01:CDS
TCAACGGACAACTTGCAAAT+TGG 0.320331 3:-92705352 MsG0380017154.01.T01:CDS
CTACCCATCATCACAGTATA+AGG 0.343091 3:+92705096 None:intergenic
CATTCTGGTTCGGTTGTTTA+CGG 0.353477 3:+92705329 None:intergenic
GGAAACCATCTATTGTGAAT+AGG 0.358486 3:+92705606 None:intergenic
ATAAACCTATTCACAATAGA+TGG 0.359431 3:-92705611 MsG0380017154.01.T01:CDS
CTTCCTCCTCCTTCTTTACC+TGG 0.359731 3:+92704970 None:intergenic
TCAAAGAATGATTGAACAAT+AGG 0.361615 3:+92705828 None:intergenic
ATGATTGGACCATAACCTTC+TGG 0.364910 3:+92705585 None:intergenic
AGATGAAGAGTTCATGAATT+CGG 0.367038 3:-92704896 MsG0380017154.01.T01:CDS
ATCTGAGAAAATGATGTTGC+TGG 0.375240 3:-92704923 MsG0380017154.01.T01:CDS
TCACCAAGGTTCAATATGTA+TGG 0.377799 3:-92705072 MsG0380017154.01.T01:CDS
TTCACCACTCACCGCATAGA+TGG 0.382856 3:+92705272 None:intergenic
TGAATAGGTTTATGTGAAAT+CGG 0.391414 3:+92705621 None:intergenic
TTGGTGATGGAACTGGTTAT+TGG 0.394500 3:-92705698 MsG0380017154.01.T01:CDS
ATCACCAATACAATGATTCA+TGG 0.399519 3:+92705713 None:intergenic
GTCGATTGCGAAAACAGTCC+TGG 0.405346 3:-92705918 MsG0380017154.01.T01:CDS
GGATATGCTAACAAATTTGA+TGG 0.406437 3:-92705009 MsG0380017154.01.T01:CDS
AGATGGTTTCCAGAAGGTTA+TGG 0.413058 3:-92705594 MsG0380017154.01.T01:CDS
TTCCATTTCTATCCCCTCTC+AGG 0.425196 3:-92705990 MsG0380017154.01.T01:CDS
ACAATAGGTGGCACATCAAC+AGG 0.427451 3:+92705843 None:intergenic
AGTGTTTCAGTTTCTCTACT+AGG 0.438345 3:+92706030 None:intergenic
GGTTCTCTATGAAATGTTGT+TGG 0.438871 3:+92706051 None:intergenic
TGATCCTTATACTGTGATGA+TGG 0.442813 3:-92705100 MsG0380017154.01.T01:CDS
GAGCTGGTTCCATTCTGGTT+CGG 0.444220 3:+92705319 None:intergenic
CATTGTATTGGTGATGGAAC+TGG 0.455215 3:-92705705 MsG0380017154.01.T01:CDS
AGTGTAAGAATGAGGATCTT+CGG 0.458409 3:+92705947 None:intergenic
AATGGAAAAGTGTGAGGGAA+AGG 0.463707 3:+92706006 None:intergenic
TTGAAAGGAAGGTTGATGAT+TGG 0.467597 3:+92705570 None:intergenic
TGATAAAGTGGTGCGAAATT+CGG 0.474906 3:-92705172 MsG0380017154.01.T01:CDS
AGAGTTCATGAATTCGGTTT+CGG 0.476951 3:-92704890 MsG0380017154.01.T01:CDS
GAATTCCCAAAATATTCCTT+AGG 0.479625 3:+92705294 None:intergenic
CATCATAATTTGCAATAGCC+AGG 0.479956 3:+92705193 None:intergenic
CAATAATTCAGTAACTTGAA+TGG 0.486762 3:+92705758 None:intergenic
AACAACGGTAGGGGAATTGC+TGG 0.488208 3:-92705250 MsG0380017154.01.T01:CDS
CGTCGTGATGTTTGAAAGGA+AGG 0.488568 3:+92705559 None:intergenic
ATGGAAAAGTGTGAGGGAAA+GGG 0.490102 3:+92706007 None:intergenic
GGTTTATGTGAAATCGGAAC+AGG 0.490426 3:+92705627 None:intergenic
ATCTGCATACTATATCCAAA+AGG 0.491658 3:-92706111 MsG0380017154.01.T01:CDS
TGTTCCATGAATCATTGTAT+TGG 0.493564 3:-92705717 MsG0380017154.01.T01:CDS
AAATATTCCTTAGGAAGAGC+TGG 0.504661 3:+92705303 None:intergenic
AGGTGAGAGAATATCATTGA+TGG 0.504727 3:+92705863 None:intergenic
GAATTGCTGGAAAATGATCT+AGG 0.510030 3:-92705237 MsG0380017154.01.T01:CDS
CAAGTTACTGAATTATTGGA+TGG 0.511663 3:-92705753 MsG0380017154.01.T01:CDS
TGCTGGAAAATGATCTAGGA+TGG 0.517189 3:-92705233 MsG0380017154.01.T01:CDS
TTCCCATACATATTGAACCT+TGG 0.519386 3:+92705069 None:intergenic
TATGGGAATGAATTTGGAAT+GGG 0.522099 3:-92705054 MsG0380017154.01.T01:CDS
AGATCCATTCGATACACAAA+AGG 0.526309 3:+92705129 None:intergenic
ATTTGGAATGGGAAAAGCAT+TGG 0.528592 3:-92705043 MsG0380017154.01.T01:CDS
TGGTTTAGTGATGATGATGT+TGG 0.530066 3:+92706071 None:intergenic
ATGAATCATTGTATTGGTGA+TGG 0.530598 3:-92705711 MsG0380017154.01.T01:CDS
GCATGAATAAATTTAGCTCC+AGG 0.532002 3:+92705900 None:intergenic
ACATCATATCCAGGTAAAGA+AGG 0.534700 3:-92704979 MsG0380017154.01.T01:CDS
GGAAAAGTAACATCATATCC+AGG 0.542909 3:-92704988 MsG0380017154.01.T01:CDS
AACTCGTCGTGATGTTTGAA+AGG 0.543380 3:+92705555 None:intergenic
CACAGAGCTGTCAACCATGA+TGG 0.543528 3:-92705801 MsG0380017154.01.T01:CDS
CACAATAGATGGTTTCCAGA+AGG 0.548245 3:-92705600 MsG0380017154.01.T01:CDS
CGTAAACAACCGAACCAGAA+TGG 0.549236 3:-92705328 MsG0380017154.01.T01:CDS
AGTAACAAGACGACCTGAGA+GGG 0.549751 3:+92705977 None:intergenic
GACCTGAGAGGGGATAGAAA+TGG 0.554535 3:+92705988 None:intergenic
TGCAAATTATGATGATAAAG+TGG 0.556314 3:-92705184 MsG0380017154.01.T01:CDS
ATGATCTAGGATGGGCAGCA+TGG 0.557831 3:-92705224 MsG0380017154.01.T01:CDS
CTCATCAAAGACTAAAACAA+AGG 0.560969 3:+92704859 None:intergenic
CACCAAGGTTCAATATGTAT+GGG 0.561237 3:-92705071 MsG0380017154.01.T01:CDS
TCTGCATACTATATCCAAAA+GGG 0.562084 3:-92706110 MsG0380017154.01.T01:CDS
AATGGAACCAGCTCTTCCTA+AGG 0.569685 3:-92705310 MsG0380017154.01.T01:CDS
GTCATTTACAACATGGTCAA+CGG 0.571152 3:-92705368 MsG0380017154.01.T01:CDS
GTATGGGAATGAATTTGGAA+TGG 0.573460 3:-92705055 MsG0380017154.01.T01:CDS
GAGTGGTGAAACAACGGTAG+GGG 0.575446 3:-92705259 MsG0380017154.01.T01:CDS
GATCCTTATACTGTGATGAT+GGG 0.590324 3:-92705099 MsG0380017154.01.T01:CDS
GGCAGCATGGAAGATTCACC+TGG 0.593449 3:-92705211 MsG0380017154.01.T01:CDS
AAGAATGATTGAACAATAGG+TGG 0.594549 3:+92705831 None:intergenic
AAAGCATTGGCAGTTAGAAG+TGG 0.597903 3:-92705030 MsG0380017154.01.T01:CDS
TATCCAGGTAAAGAAGGAGG+AGG 0.601869 3:-92704973 MsG0380017154.01.T01:CDS
TGAGTGGTGAAACAACGGTA+GGG 0.603070 3:-92705260 MsG0380017154.01.T01:CDS
TGCGAAATTCGGTTGAAGAG+TGG 0.613912 3:-92705161 MsG0380017154.01.T01:CDS
GATAGAAATGGAAAAGTGTG+AGG 0.622911 3:+92706000 None:intergenic
GCTGGAAAATGATCTAGGAT+GGG 0.626677 3:-92705232 MsG0380017154.01.T01:CDS
CGTGCACGTCATTTACAACA+TGG 0.627245 3:-92705375 MsG0380017154.01.T01:CDS
TGCGGTGAGTGGTGAAACAA+CGG 0.630833 3:-92705265 MsG0380017154.01.T01:CDS
GTGAGTGGTGAAACAACGGT+AGG 0.635845 3:-92705261 MsG0380017154.01.T01:CDS
AAGACATGGTGTGTCCATCA+TGG 0.638582 3:+92705787 None:intergenic
TCATATCCAGGTAAAGAAGG+AGG 0.645386 3:-92704976 MsG0380017154.01.T01:CDS
TTGAATGGACAACAAAGACA+TGG 0.674790 3:+92705773 None:intergenic
AATTCCATCTATGCGGTGAG+TGG 0.681160 3:-92705276 MsG0380017154.01.T01:CDS
ATAGAAATGGAAAAGTGTGA+GGG 0.681846 3:+92706001 None:intergenic
CATCATGGTTGACAGCTCTG+TGG 0.688486 3:+92705802 None:intergenic
TAGTAACAAGACGACCTGAG+AGG 0.695039 3:+92705976 None:intergenic
TGATGATGGGTAGCTCACCA+AGG 0.697945 3:-92705086 MsG0380017154.01.T01:CDS
GTAACAAGACGACCTGAGAG+GGG 0.714877 3:+92705978 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATATGTATGGGAATGAATT+TGG - Chr3:92705926-92705945 MsG0380017154.01.T01:CDS 25.0%
! AATATTCTCTCTCTCAAAAT+AGG + Chr3:92705476-92705495 None:intergenic 25.0%
! ATAAACCTATTCACAATAGA+TGG - Chr3:92705375-92705394 MsG0380017154.01.T01:CDS 25.0%
! CAATAATTCAGTAACTTGAA+TGG + Chr3:92705231-92705250 None:intergenic 25.0%
! TCAAAGAATGATTGAACAAT+AGG + Chr3:92705161-92705180 None:intergenic 25.0%
! TGAATAGGTTTATGTGAAAT+CGG + Chr3:92705368-92705387 None:intergenic 25.0%
! TGCAAATTATGATGATAAAG+TGG - Chr3:92705802-92705821 MsG0380017154.01.T01:CDS 25.0%
!! CATTCAAGTTACTGAATTAT+TGG - Chr3:92705229-92705248 MsG0380017154.01.T01:CDS 25.0%
!!! TTATTGGATGGTGTTTTTAT+AGG - Chr3:92705245-92705264 MsG0380017154.01.T01:CDS 25.0%
AAGAATGATTGAACAATAGG+TGG + Chr3:92705158-92705177 None:intergenic 30.0%
AATCTTTATCAGCACTAGTT+TGG - Chr3:92705576-92705595 MsG0380017154.01.T01:CDS 30.0%
ACAAAAACAGTGTAAGAATG+AGG + Chr3:92705050-92705069 None:intergenic 30.0%
AGATGAAGAGTTCATGAATT+CGG - Chr3:92706090-92706109 MsG0380017154.01.T01:CDS 30.0%
ATCACCAATACAATGATTCA+TGG + Chr3:92705276-92705295 None:intergenic 30.0%
ATCTGCATACTATATCCAAA+AGG - Chr3:92704875-92704894 MsG0380017154.01.T01:CDS 30.0%
CAAGTTACTGAATTATTGGA+TGG - Chr3:92705233-92705252 MsG0380017154.01.T01:CDS 30.0%
GAATTCCCAAAATATTCCTT+AGG + Chr3:92705695-92705714 None:intergenic 30.0%
GGATATGCTAACAAATTTGA+TGG - Chr3:92705977-92705996 MsG0380017154.01.T01:CDS 30.0%
TCTGCATACTATATCCAAAA+GGG - Chr3:92704876-92704895 MsG0380017154.01.T01:CDS 30.0%
TGTTCCATGAATCATTGTAT+TGG - Chr3:92705269-92705288 MsG0380017154.01.T01:CDS 30.0%
! ATAGAAATGGAAAAGTGTGA+GGG + Chr3:92704988-92705007 None:intergenic 30.0%
! ATGAATCATTGTATTGGTGA+TGG - Chr3:92705275-92705294 MsG0380017154.01.T01:CDS 30.0%
! CTCTTCCTAAGGAATATTTT+GGG - Chr3:92705687-92705706 MsG0380017154.01.T01:CDS 30.0%
! TATGGGAATGAATTTGGAAT+GGG - Chr3:92705932-92705951 MsG0380017154.01.T01:CDS 30.0%
AAATATTCCTTAGGAAGAGC+TGG + Chr3:92705686-92705705 None:intergenic 35.0%
ACATCATATCCAGGTAAAGA+AGG - Chr3:92706007-92706026 MsG0380017154.01.T01:CDS 35.0%
AGAGTTCATGAATTCGGTTT+CGG - Chr3:92706096-92706115 MsG0380017154.01.T01:CDS 35.0%
AGATCCATTCGATACACAAA+AGG + Chr3:92705860-92705879 None:intergenic 35.0%
AGGTGAGAGAATATCATTGA+TGG + Chr3:92705126-92705145 None:intergenic 35.0%
AGTGTAAGAATGAGGATCTT+CGG + Chr3:92705042-92705061 None:intergenic 35.0%
AGTGTTTCAGTTTCTCTACT+AGG + Chr3:92704959-92704978 None:intergenic 35.0%
ATCTGAGAAAATGATGTTGC+TGG - Chr3:92706063-92706082 MsG0380017154.01.T01:CDS 35.0%
CACCAAGGTTCAATATGTAT+GGG - Chr3:92705915-92705934 MsG0380017154.01.T01:CDS 35.0%
GAATTGCTGGAAAATGATCT+AGG - Chr3:92705749-92705768 MsG0380017154.01.T01:CDS 35.0%
GATCCTTATACTGTGATGAT+GGG - Chr3:92705887-92705906 MsG0380017154.01.T01:CDS 35.0%
GGAAAAGTAACATCATATCC+AGG - Chr3:92705998-92706017 MsG0380017154.01.T01:CDS 35.0%
GGAAACCATCTATTGTGAAT+AGG + Chr3:92705383-92705402 None:intergenic 35.0%
GGTTCTCTATGAAATGTTGT+TGG + Chr3:92704938-92704957 None:intergenic 35.0%
GTATGGGAATGAATTTGGAA+TGG - Chr3:92705931-92705950 MsG0380017154.01.T01:CDS 35.0%
GTCATTTACAACATGGTCAA+CGG - Chr3:92705618-92705637 MsG0380017154.01.T01:CDS 35.0%
TCACCAAGGTTCAATATGTA+TGG - Chr3:92705914-92705933 MsG0380017154.01.T01:CDS 35.0%
TGATAAAGTGGTGCGAAATT+CGG - Chr3:92705814-92705833 MsG0380017154.01.T01:CDS 35.0%
TGATCCTTATACTGTGATGA+TGG - Chr3:92705886-92705905 MsG0380017154.01.T01:CDS 35.0%
TTCCCATACATATTGAACCT+TGG + Chr3:92705920-92705939 None:intergenic 35.0%
TTGAATGGACAACAAAGACA+TGG + Chr3:92705216-92705235 None:intergenic 35.0%
! ATTTGGAATGGGAAAAGCAT+TGG - Chr3:92705943-92705962 MsG0380017154.01.T01:CDS 35.0%
! CATCATAATTTGCAATAGCC+AGG + Chr3:92705796-92705815 None:intergenic 35.0%
! GATAGAAATGGAAAAGTGTG+AGG + Chr3:92704989-92705008 None:intergenic 35.0%
! GCATGAATAAATTTAGCTCC+AGG + Chr3:92705089-92705108 None:intergenic 35.0%
! GCTCTTCCTAAGGAATATTT+TGG - Chr3:92705686-92705705 MsG0380017154.01.T01:CDS 35.0%
! TGGTTTAGTGATGATGATGT+TGG + Chr3:92704918-92704937 None:intergenic 35.0%
! TTCTCCTTTTGTGTATCGAA+TGG - Chr3:92705853-92705872 MsG0380017154.01.T01:CDS 35.0%
!! TTGAAAGGAAGGTTGATGAT+TGG + Chr3:92705419-92705438 None:intergenic 35.0%
!! TTTTGAAGAGAAGACCCTTT+TGG + Chr3:92704893-92704912 None:intergenic 35.0%
!! TTTTGGGAATTCCATCTATG+CGG - Chr3:92705703-92705722 MsG0380017154.01.T01:CDS 35.0%
AACTCGTCGTGATGTTTGAA+AGG + Chr3:92705434-92705453 None:intergenic 40.0%
AGATGGTTTCCAGAAGGTTA+TGG - Chr3:92705392-92705411 MsG0380017154.01.T01:CDS 40.0%
ATGATTGGACCATAACCTTC+TGG + Chr3:92705404-92705423 None:intergenic 40.0%
CACAATAGATGGTTTCCAGA+AGG - Chr3:92705386-92705405 MsG0380017154.01.T01:CDS 40.0%
CTACCCATCATCACAGTATA+AGG + Chr3:92705893-92705912 None:intergenic 40.0%
GGTTTATGTGAAATCGGAAC+AGG + Chr3:92705362-92705381 None:intergenic 40.0%
TCAACGGACAACTTGCAAAT+TGG - Chr3:92705634-92705653 MsG0380017154.01.T01:CDS 40.0%
TCATATCCAGGTAAAGAAGG+AGG - Chr3:92706010-92706029 MsG0380017154.01.T01:CDS 40.0%
TGCTGGAAAATGATCTAGGA+TGG - Chr3:92705753-92705772 MsG0380017154.01.T01:CDS 40.0%
! AATGGAAAAGTGTGAGGGAA+AGG + Chr3:92704983-92705002 None:intergenic 40.0%
! ATGGAAAAGTGTGAGGGAAA+GGG + Chr3:92704982-92705001 None:intergenic 40.0%
! GCTGGAAAATGATCTAGGAT+GGG - Chr3:92705754-92705773 MsG0380017154.01.T01:CDS 40.0%
!! AAAGCATTGGCAGTTAGAAG+TGG - Chr3:92705956-92705975 MsG0380017154.01.T01:CDS 40.0%
!! CATTCTGGTTCGGTTGTTTA+CGG + Chr3:92705660-92705679 None:intergenic 40.0%
!! CATTGTATTGGTGATGGAAC+TGG - Chr3:92705281-92705300 MsG0380017154.01.T01:CDS 40.0%
!! TTGGTGATGGAACTGGTTAT+TGG - Chr3:92705288-92705307 MsG0380017154.01.T01:CDS 40.0%
AAGACATGGTGTGTCCATCA+TGG + Chr3:92705202-92705221 None:intergenic 45.0%
AATGGAACCAGCTCTTCCTA+AGG - Chr3:92705676-92705695 MsG0380017154.01.T01:CDS 45.0%
AATTCCATCTATGCGGTGAG+TGG - Chr3:92705710-92705729 MsG0380017154.01.T01:CDS 45.0%
ACAATAGGTGGCACATCAAC+AGG + Chr3:92705146-92705165 None:intergenic 45.0%
AGTAACAAGACGACCTGAGA+GGG + Chr3:92705012-92705031 None:intergenic 45.0%
CGTAAACAACCGAACCAGAA+TGG - Chr3:92705658-92705677 MsG0380017154.01.T01:CDS 45.0%
CGTCGTGATGTTTGAAAGGA+AGG + Chr3:92705430-92705449 None:intergenic 45.0%
CGTGCACGTCATTTACAACA+TGG - Chr3:92705611-92705630 MsG0380017154.01.T01:CDS 45.0%
TAGTAACAAGACGACCTGAG+AGG + Chr3:92705013-92705032 None:intergenic 45.0%
TATCCAGGTAAAGAAGGAGG+AGG - Chr3:92706013-92706032 MsG0380017154.01.T01:CDS 45.0%
TGAGTGGTGAAACAACGGTA+GGG - Chr3:92705726-92705745 MsG0380017154.01.T01:CDS 45.0%
TGCGAAATTCGGTTGAAGAG+TGG - Chr3:92705825-92705844 MsG0380017154.01.T01:CDS 45.0%
TTCCATTTCTATCCCCTCTC+AGG - Chr3:92704996-92705015 MsG0380017154.01.T01:CDS 45.0%
AACAACGGTAGGGGAATTGC+TGG - Chr3:92705736-92705755 MsG0380017154.01.T01:CDS 50.0%
CACAGAGCTGTCAACCATGA+TGG - Chr3:92705185-92705204 MsG0380017154.01.T01:CDS 50.0%
CATCATGGTTGACAGCTCTG+TGG + Chr3:92705187-92705206 None:intergenic 50.0%
CTTCCTCCTCCTTCTTTACC+TGG + Chr3:92706019-92706038 None:intergenic 50.0%
GACCTGAGAGGGGATAGAAA+TGG + Chr3:92705001-92705020 None:intergenic 50.0%
GAGTGGTGAAACAACGGTAG+GGG - Chr3:92705727-92705746 MsG0380017154.01.T01:CDS 50.0%
GTAACAAGACGACCTGAGAG+GGG + Chr3:92705011-92705030 None:intergenic 50.0%
GTCGATTGCGAAAACAGTCC+TGG - Chr3:92705068-92705087 MsG0380017154.01.T01:CDS 50.0%
GTGAGTGGTGAAACAACGGT+AGG - Chr3:92705725-92705744 MsG0380017154.01.T01:CDS 50.0%
TGCGGTGAGTGGTGAAACAA+CGG - Chr3:92705721-92705740 MsG0380017154.01.T01:CDS 50.0%
TTCACCACTCACCGCATAGA+TGG + Chr3:92705717-92705736 None:intergenic 50.0%
! AGGAAGAGCTGGTTCCATTC+TGG + Chr3:92705675-92705694 None:intergenic 50.0%
! ATGATCTAGGATGGGCAGCA+TGG - Chr3:92705762-92705781 MsG0380017154.01.T01:CDS 50.0%
! GAGCTGGTTCCATTCTGGTT+CGG + Chr3:92705670-92705689 None:intergenic 50.0%
!! TGATGATGGGTAGCTCACCA+AGG - Chr3:92705900-92705919 MsG0380017154.01.T01:CDS 50.0%
GGCAGCATGGAAGATTCACC+TGG - Chr3:92705775-92705794 MsG0380017154.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 92704870 92706138 92704870 ID=MsG0380017154.01;Name=MsG0380017154.01
Chr3 mRNA 92704870 92706138 92704870 ID=MsG0380017154.01.T01;Parent=MsG0380017154.01;Name=MsG0380017154.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|422
Chr3 exon 92704870 92706138 92704870 ID=MsG0380017154.01.T01:exon:15721;Parent=MsG0380017154.01.T01
Chr3 CDS 92704870 92706138 92704870 ID=MsG0380017154.01.T01:cds;Parent=MsG0380017154.01.T01
Gene Sequence

>MsG0380017154.01.T01

ATGTTATCTGCATACTATATCCAAAAGGGTCTTCTCTTCAAAAAACCAACATCATCATCACTAAACCAACAACATTTCATAGAGAACCTAGTAGAGAAACTGAAACACTCCCTTTCCCTCACACTTTTCCATTTCTATCCCCTCTCAGGTCGTCTTGTTACTAAAAAATCCGAAGATCCTCATTCTTACACTGTTTTTGTCGATTGCGAAAACAGTCCTGGAGCTAAATTTATTCATGCTACTTTAGATATCACCATCAATGATATTCTCTCACCTGTTGATGTGCCACCTATTGTTCAATCATTCTTTGATCACCACAGAGCTGTCAACCATGATGGACACACCATGTCTTTGTTGTCCATTCAAGTTACTGAATTATTGGATGGTGTTTTTATAGGTTGTTCCATGAATCATTGTATTGGTGATGGAACTGGTTATTGGAATTTCTTTAACTCATTTTCTGAGATATTTCAAAATGATGTTCATGTTCCTGTTCCGATTTCACATAAACCTATTCACAATAGATGGTTTCCAGAAGGTTATGGTCCAATCATCAACCTTCCTTTCAAACATCACGACGAGTTTATTCATAGATATGAAACACCTATTTTGAGAGAGAGAATATTTCATTTTTCAGCAGAGTCTATTGCAAAACTTAAAGCAAAAGCTAACAAAGAGTCTGATACAAACAAAATCTCTTCTTTTCAATCTTTATCAGCACTAGTTTGGAGATCTCTAACGCGTGCACGTCATTTACAACATGGTCAACGGACAACTTGCAAATTGGCCGTAAACAACCGAACCAGAATGGAACCAGCTCTTCCTAAGGAATATTTTGGGAATTCCATCTATGCGGTGAGTGGTGAAACAACGGTAGGGGAATTGCTGGAAAATGATCTAGGATGGGCAGCATGGAAGATTCACCTGGCTATTGCAAATTATGATGATAAAGTGGTGCGAAATTCGGTTGAAGAGTGGTTACGTTCTCCTTTTGTGTATCGAATGGATCTGTTCTTTGATCCTTATACTGTGATGATGGGTAGCTCACCAAGGTTCAATATGTATGGGAATGAATTTGGAATGGGAAAAGCATTGGCAGTTAGAAGTGGATATGCTAACAAATTTGATGGAAAAGTAACATCATATCCAGGTAAAGAAGGAGGAGGAAGCATTGATCTTGAAGTGTGTCTTTCATCTGAGAAAATGATGTTGCTGGAAACAGATGAAGAGTTCATGAATTCGGTTTCGGTGTTTAATCCTTTGTTTTAG
Protein sequence

>MsG0380017154.01.T01

MLSAYYIQKGLLFKKPTSSSLNQQHFIENLVEKLKHSLSLTLFHFYPLSGRLVTKKSEDPHSYTVFVDCENSPGAKFIHATLDITINDILSPVDVPPIVQSFFDHHRAVNHDGHTMSLLSIQVTELLDGVFIGCSMNHCIGDGTGYWNFFNSFSEIFQNDVHVPVPISHKPIHNRWFPEGYGPIINLPFKHHDEFIHRYETPILRERIFHFSAESIAKLKAKANKESDTNKISSFQSLSALVWRSLTRARHLQHGQRTTCKLAVNNRTRMEPALPKEYFGNSIYAVSGETTVGELLENDLGWAAWKIHLAIANYDDKVVRNSVEEWLRSPFVYRMDLFFDPYTVMMGSSPRFNMYGNEFGMGKALAVRSGYANKFDGKVTSYPGKEGGGSIDLEVCLSSEKMMLLETDEEFMNSVSVFNPLF*