AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045887.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045887.01.T01 MTR_4g094828 90.949 453 39 2 1 453 1 451 0.0 853
MsG0880045887.01.T01 MTR_4g022290 83.850 452 62 3 1 450 1 443 0.0 768
MsG0880045887.01.T01 MTR_8g079710 74.558 452 100 7 1 448 1 441 0.0 666
MsG0880045887.01.T01 MTR_4g094835 54.746 453 181 12 1 444 1 438 2.93e-167 478
MsG0880045887.01.T01 MTR_4g094842 53.509 456 184 8 1 444 1 440 2.74e-160 461
MsG0880045887.01.T01 MTR_0190s0020 49.781 456 211 5 1 452 1 442 8.24e-152 439
MsG0880045887.01.T01 MTR_3g088420 51.316 456 195 11 1 452 1 433 8.70e-152 438
MsG0880045887.01.T01 MTR_4g022800 51.188 463 194 13 1 452 1 442 1.30e-149 433
MsG0880045887.01.T01 MTR_4g022825 51.310 458 197 12 1 451 1 439 8.10e-149 431
MsG0880045887.01.T01 MTR_3g026310 50.535 467 197 11 1 452 1 448 2.62e-148 430
MsG0880045887.01.T01 MTR_4g022720 50.989 455 196 13 1 450 1 433 4.92e-148 429
MsG0880045887.01.T01 MTR_4g094838 50.221 452 182 11 6 444 3 424 6.87e-146 423
MsG0880045887.01.T01 MTR_8g041770 49.563 458 208 9 1 452 1 441 1.03e-145 423
MsG0880045887.01.T01 MTR_7g026860 50.545 459 195 13 1 450 1 436 2.06e-144 420
MsG0880045887.01.T01 MTR_4g022670 50.435 460 201 12 1 452 1 441 2.09e-144 420
MsG0880045887.01.T01 MTR_4g094832 54.712 382 147 9 73 444 57 422 6.81e-138 403
MsG0880045887.01.T01 MTR_4g022760 44.227 459 187 10 1 452 1 397 1.11e-118 353
MsG0880045887.01.T01 MTR_6g016815 41.630 454 176 10 1 452 15 381 7.31e-104 314
MsG0880045887.01.T01 MTR_3g101900 37.443 438 243 13 29 453 30 449 3.10e-81 258
MsG0880045887.01.T01 MTR_3g101930 37.415 441 236 13 25 444 26 447 1.21e-80 257
MsG0880045887.01.T01 MTR_3g102020 36.481 466 257 15 6 453 8 452 2.03e-77 248
MsG0880045887.01.T01 MTR_3g102010 36.404 445 249 13 25 453 26 452 8.02e-77 247
MsG0880045887.01.T01 MTR_3g101970 35.491 448 240 14 23 453 23 438 2.19e-75 243
MsG0880045887.01.T01 MTR_3g101960 36.446 439 243 13 23 444 23 442 3.82e-75 243
MsG0880045887.01.T01 MTR_8g040760 53.456 217 93 4 1 212 1 214 1.25e-69 220
MsG0880045887.01.T01 MTR_5g081960 32.922 486 264 15 6 444 8 478 1.16e-64 216
MsG0880045887.01.T01 MTR_4g094825 56.085 189 75 5 20 202 83 269 2.18e-62 207
MsG0880045887.01.T01 MTR_4g094825 50.000 146 59 4 300 444 251 383 1.83e-36 138
MsG0880045887.01.T01 MTR_3g070170 31.410 468 271 12 13 448 17 466 1.93e-60 204
MsG0880045887.01.T01 MTR_4g015180 31.474 502 269 14 8 452 4 487 7.16e-60 203
MsG0880045887.01.T01 MTR_7g026850 59.868 152 59 2 34 184 12 162 4.09e-57 187
MsG0880045887.01.T01 MTR_8g040780 45.205 219 105 7 230 444 17 224 2.64e-50 171
MsG0880045887.01.T01 MTR_6g034495 66.379 116 37 1 70 183 45 160 2.54e-46 158
MsG0880045887.01.T01 MTR_3g102030 32.759 348 178 13 70 405 17 320 2.01e-43 155
MsG0880045887.01.T01 MTR_6g045553 29.018 448 272 12 25 442 31 462 4.20e-38 144
MsG0880045887.01.T01 MTR_7g026770 40.526 190 81 4 146 335 132 289 4.62e-34 129
MsG0880045887.01.T01 MTR_7g026830 67.073 82 27 0 89 170 16 97 1.46e-32 119
MsG0880045887.01.T01 MTR_6g034485 40.288 139 69 3 191 329 1 125 7.49e-27 105
MsG0880045887.01.T01 MTR_8g075610 25.635 394 255 15 59 441 47 413 3.41e-26 110
MsG0880045887.01.T01 MTR_2g105330 25.547 411 263 15 46 439 39 423 5.59e-25 107
MsG0880045887.01.T01 MTR_2g064495 25.547 411 263 15 46 439 39 423 1.05e-24 106
MsG0880045887.01.T01 MTR_4g007540 26.108 406 256 15 46 433 39 418 4.26e-24 104
MsG0880045887.01.T01 MTR_7g085930 25.424 413 264 13 49 431 49 447 1.72e-21 97.1
MsG0880045887.01.T01 MTR_8g096530 26.816 358 212 14 59 400 59 382 1.98e-21 96.7
MsG0880045887.01.T01 MTR_3g049150 24.408 422 263 16 46 437 38 433 4.89e-21 95.5
MsG0880045887.01.T01 MTR_7g027175 25.879 398 262 14 49 439 58 429 5.85e-21 95.1
MsG0880045887.01.T01 MTR_3g098980 25.000 356 224 14 59 402 66 390 2.50e-20 93.2
MsG0880045887.01.T01 MTR_1g040175 24.317 366 245 12 45 401 45 387 3.44e-20 93.2
MsG0880045887.01.T01 MTR_7g085990 24.096 415 283 11 49 441 45 449 1.05e-19 91.7
MsG0880045887.01.T01 MTR_5g026320 25.532 470 275 22 6 444 20 445 1.67e-19 90.9
MsG0880045887.01.T01 MTR_7g014200 23.427 461 275 16 27 439 28 458 1.18e-17 85.5
MsG0880045887.01.T01 MTR_1g040215 24.619 394 244 15 31 401 24 387 3.88e-17 84.0
MsG0880045887.01.T01 MTR_2g089765 22.588 425 253 16 26 400 36 434 1.26e-16 82.4
MsG0880045887.01.T01 MTR_4g097090 24.356 427 253 19 46 433 45 440 6.56e-16 80.1
MsG0880045887.01.T01 MTR_7g014610 22.484 467 278 17 32 443 32 469 2.84e-15 78.2
MsG0880045887.01.T01 MTR_7g014250 22.906 406 257 14 33 404 30 413 2.98e-15 78.2
MsG0880045887.01.T01 MTR_6g015315 23.308 399 257 15 33 403 30 407 3.56e-15 77.8
MsG0880045887.01.T01 MTR_7g014160 24.390 451 270 18 29 433 30 455 3.72e-15 77.8
MsG0880045887.01.T01 MTR_5g090880 24.064 374 227 12 59 401 67 414 3.75e-15 77.8
MsG0880045887.01.T01 MTR_8g012750 24.279 416 258 17 26 400 23 422 5.02e-15 77.4
MsG0880045887.01.T01 MTR_6g015815 23.558 416 247 15 27 403 32 415 6.74e-15 77.0
MsG0880045887.01.T01 MTR_7g026780 41.593 113 39 5 340 452 26 111 8.09e-15 70.9
MsG0880045887.01.T01 MTR_6g015320 21.749 423 264 15 24 403 11 409 1.01e-14 76.3
MsG0880045887.01.T01 MTR_4g127650 23.951 405 248 20 59 433 66 440 1.53e-14 75.9
MsG0880045887.01.T01 MTR_1g081660 23.383 402 243 15 29 401 31 396 1.67e-14 75.9
MsG0880045887.01.T01 MTR_3g112180 23.133 415 285 13 33 439 38 426 2.23e-14 75.5
MsG0880045887.01.T01 MTR_7g014230 24.390 410 258 15 27 404 26 415 3.13e-14 75.1
MsG0880045887.01.T01 MTR_6g015830 24.505 404 254 14 29 403 34 415 4.22e-14 74.3
MsG0880045887.01.T01 MTR_6g015850 23.114 411 252 16 27 403 32 412 4.42e-14 74.3
MsG0880045887.01.T01 MTR_5g090830 23.573 403 244 14 37 401 36 412 5.13e-14 74.3
MsG0880045887.01.T01 MTR_2g089670 24.557 395 240 18 41 400 58 429 1.21e-13 73.2
MsG0880045887.01.T01 MTR_6g053810 26.948 308 203 11 59 360 71 362 5.50e-13 71.2
MsG0880045887.01.T01 MTR_7g114490 24.379 443 274 19 31 442 25 437 1.07e-12 70.1
MsG0880045887.01.T01 MTR_8g079980 24.543 383 248 16 52 425 52 402 1.55e-12 69.7
MsG0880045887.01.T01 MTR_2g089650 23.261 417 276 16 42 429 42 443 2.56e-12 68.9
MsG0880045887.01.T01 MTR_7g114520 23.441 401 260 16 31 408 26 402 2.68e-12 68.9
MsG0880045887.01.T01 MTR_7g114550 28.326 233 142 9 31 247 25 248 1.02e-11 67.0
MsG0880045887.01.T01 MTR_7g026880 56.364 55 21 2 398 452 2 53 3.00e-11 58.9
MsG0880045887.01.T01 MTR_7g014620 22.172 442 265 17 29 433 15 414 9.68e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045887.01.T01 AT5G67150 38.528 462 253 14 4 453 5 447 2.60e-95 295
MsG0880045887.01.T01 AT3G50270 36.364 462 268 13 2 453 4 449 1.12e-86 272
MsG0880045887.01.T01 AT5G67160 38.173 427 230 13 25 444 25 424 1.35e-85 269
MsG0880045887.01.T01 AT3G50300 37.527 469 252 15 1 451 1 446 7.13e-85 268
MsG0880045887.01.T01 AT3G50280 34.368 451 273 10 1 444 1 435 2.54e-80 256
MsG0880045887.01.T01 AT5G38130 36.343 443 251 11 1 433 21 442 1.85e-79 254
MsG0880045887.01.T01 AT5G42830 34.852 439 254 12 29 453 30 450 1.94e-75 243
MsG0880045887.01.T01 AT5G07860 35.402 435 252 10 29 451 31 448 1.79e-70 230
MsG0880045887.01.T01 AT5G07850 33.864 440 258 12 29 452 31 453 2.46e-69 228
MsG0880045887.01.T01 AT5G07870 35.650 446 250 13 29 452 31 461 2.63e-66 220
MsG0880045887.01.T01 AT2G39980 31.524 479 266 12 19 452 19 480 1.86e-65 218
MsG0880045887.01.T01 AT5G01210 31.692 467 266 11 22 452 20 469 2.55e-65 218
MsG0880045887.01.T01 AT3G50280 35.463 313 184 7 130 438 1 299 6.71e-56 188
MsG0880045887.01.T01 AT3G50290 38.078 281 156 7 124 398 1 269 2.20e-49 170
MsG0880045887.01.T01 AT5G23940 31.264 435 267 11 25 441 21 441 6.72e-47 169
MsG0880045887.01.T01 AT5G41040 26.617 402 254 16 59 448 69 441 1.75e-25 108
MsG0880045887.01.T01 AT5G41040 26.667 405 250 16 59 448 85 457 1.83e-25 108
MsG0880045887.01.T01 AT3G48720 26.077 418 264 18 39 441 37 424 2.83e-24 105
MsG0880045887.01.T01 AT5G48930 26.699 412 246 18 46 433 39 418 1.84e-23 102
MsG0880045887.01.T01 AT5G63560 24.359 390 244 13 59 434 61 413 1.73e-22 99.8
MsG0880045887.01.T01 AT2G19070 26.577 444 270 21 29 441 25 443 5.79e-20 92.4
MsG0880045887.01.T01 AT1G78990 25.203 369 233 13 48 401 56 396 4.05e-18 87.0
MsG0880045887.01.T01 AT3G29590 22.727 440 294 14 27 443 26 442 9.01e-18 85.9
MsG0880045887.01.T01 AT5G47950 25.888 394 233 14 55 436 57 403 5.66e-17 83.2
MsG0880045887.01.T01 AT1G32910 24.860 358 231 13 59 401 65 399 5.96e-17 83.2
MsG0880045887.01.T01 AT5G39080 23.095 420 279 16 13 403 12 416 4.77e-16 80.5
MsG0880045887.01.T01 AT3G03480 25.381 394 241 16 31 401 34 397 8.73e-15 76.6
MsG0880045887.01.T01 AT5G16410 23.940 401 245 16 24 401 56 419 9.51e-15 76.6
MsG0880045887.01.T01 AT5G23970 24.515 412 243 17 45 434 38 403 1.72e-14 75.5
MsG0880045887.01.T01 AT3G26040 26.055 403 231 15 53 433 53 410 1.76e-14 75.5
MsG0880045887.01.T01 AT1G24420 26.074 349 200 17 70 403 76 381 2.60e-14 75.1
MsG0880045887.01.T01 AT1G24420 26.074 349 200 17 70 403 76 381 2.60e-14 75.1
MsG0880045887.01.T01 AT1G03390 24.455 413 260 21 47 434 59 444 1.57e-12 69.7
MsG0880045887.01.T01 AT5G47980 24.286 420 236 17 58 445 60 429 7.89e-12 67.4
MsG0880045887.01.T01 AT5G39090 23.671 414 264 17 13 400 12 399 1.48e-11 66.6
MsG0880045887.01.T01 AT5G61160 21.481 405 266 15 26 401 22 403 2.73e-11 65.9
MsG0880045887.01.T01 AT1G31490 24.318 403 250 17 18 401 18 384 9.42e-11 63.9

Find 90 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTCCTTATGATATTT+TGG 0.223517 8:-66000418 MsG0880045887.01.T01:CDS
TCAAAAGCTAATGCAGAAAT+TGG 0.276477 8:-66000969 MsG0880045887.01.T01:CDS
GGATCTCAAATATCTTAAAA+TGG 0.314276 8:-66001615 MsG0880045887.01.T01:CDS
GTTGGATTTAAAAGAAATAT+TGG 0.317499 8:-66000492 MsG0880045887.01.T01:CDS
GTGTAGACGCCAAATCATTT+TGG 0.321096 8:-66001217 MsG0880045887.01.T01:CDS
ATGGTCGATTTGGTTTGTTT+TGG 0.322357 8:+66001559 None:intergenic
AATGCAAGTAGGGAAATTAA+TGG 0.338622 8:-66000736 MsG0880045887.01.T01:CDS
ACACCATTTCTAACTGCAAT+AGG 0.350294 8:+66000513 None:intergenic
TTTGGCATTTCATCAACTCT+TGG 0.368556 8:-66001199 MsG0880045887.01.T01:CDS
TTGGCATTTCATCAACTCTT+GGG 0.376104 8:-66001198 MsG0880045887.01.T01:CDS
ATGGAACATGAGGCGTTGTT+AGG 0.379377 8:+66001458 None:intergenic
TTTGTTTAAGATGGTCGATT+TGG 0.383044 8:+66001549 None:intergenic
GTTGAAAACCCCGTCTATAT+CGG 0.384392 8:-66000622 MsG0880045887.01.T01:CDS
AAGAATAATGAGGAAACAAT+AGG 0.387662 8:+66001347 None:intergenic
TCACCACGGTTCAATTATTA+CGG 0.389485 8:-66000558 MsG0880045887.01.T01:CDS
ATGGGACGATTAATGTTGTT+TGG 0.400321 8:+66001116 None:intergenic
TCGTGTTACCCGTAGATTGT+AGG 0.401751 8:-66000827 MsG0880045887.01.T01:CDS
TTACGGTAATGACTTTGGTT+GGG 0.403096 8:-66000541 MsG0880045887.01.T01:CDS
TTAATCGACGGTATCTTCAT+TGG 0.408333 8:-66001260 MsG0880045887.01.T01:CDS
GGAAGTGGAATCGGAGCAAC+TGG 0.414902 8:-66000768 MsG0880045887.01.T01:CDS
GATGAAATGCCAAAATGATT+TGG 0.418439 8:+66001208 None:intergenic
AAAATGGCACCAATCCAAAA+AGG 0.433344 8:-66001599 MsG0880045887.01.T01:CDS
AATTATTACGGTAATGACTT+TGG 0.438421 8:-66000546 MsG0880045887.01.T01:CDS
CTTTGTGAAAGGAAACTGTA+TGG 0.444172 8:+66001097 None:intergenic
TGGAAAGTGTTTGTTTAAGA+TGG 0.447960 8:+66001540 None:intergenic
ATTACGGTAATGACTTTGGT+TGG 0.453916 8:-66000542 MsG0880045887.01.T01:CDS
GAGGAACATACTTTGGTTGA+AGG 0.461926 8:+66001369 None:intergenic
CATTCTTGTCCTACAATCTA+CGG 0.463033 8:+66000818 None:intergenic
TACGGTAATGACTTTGGTTG+GGG 0.464169 8:-66000540 MsG0880045887.01.T01:CDS
ATCAAAATCTCATCATGAAA+AGG 0.465218 8:-66000667 MsG0880045887.01.T01:CDS
GGAAACCAACGTTCGAAAGA+AGG 0.465248 8:+66001137 None:intergenic
TCGAATCTTCCACTTTACTA+AGG 0.472625 8:-66001012 MsG0880045887.01.T01:CDS
AAGCCTATTGCAGTTAGAAA+TGG 0.478020 8:-66000516 MsG0880045887.01.T01:CDS
ACGAAAGCAGACCTTTCTTC+TGG 0.482415 8:+66000846 None:intergenic
ACAATAGGAGGAACATACTT+TGG 0.484514 8:+66001362 None:intergenic
ATAGCCTCCAAAATATCATA+AGG 0.495132 8:+66000411 None:intergenic
AACTTCTTTCCACCACTCGC+TGG 0.495410 8:-66001509 MsG0880045887.01.T01:CDS
GTAGGGAAATTAATGGAAAG+TGG 0.496846 8:-66000729 MsG0880045887.01.T01:CDS
GGTAGAGTTATTGTGCTTGG+TGG 0.507052 8:-66000471 MsG0880045887.01.T01:CDS
GAATTTGTTCCTTAGTAAAG+TGG 0.513443 8:+66001003 None:intergenic
GATATGTAATAATGCAAGTA+GGG 0.513879 8:-66000746 MsG0880045887.01.T01:CDS
TTAAGATATTTGAGATCCCA+TGG 0.520479 8:+66001620 None:intergenic
AAATTAATGGAAAGTGGGAT+TGG 0.520728 8:-66000723 MsG0880045887.01.T01:CDS
TACCTGGTTCCGATATAGAC+GGG 0.523820 8:+66000613 None:intergenic
AAATCCCTTCTTTCGAACGT+TGG 0.525283 8:-66001142 MsG0880045887.01.T01:CDS
TACTTATAATTAAACGCCAA+AGG 0.529613 8:+66000883 None:intergenic
TAGTGAGTAATTATGAGTCT+TGG 0.531485 8:-66000644 MsG0880045887.01.T01:CDS
ATTGGTAGAGTTATTGTGCT+TGG 0.531528 8:-66000474 MsG0880045887.01.T01:CDS
GGATATGTAATAATGCAAGT+AGG 0.531741 8:-66000747 MsG0880045887.01.T01:CDS
TAGGGAAATTAATGGAAAGT+GGG 0.533012 8:-66000728 MsG0880045887.01.T01:CDS
AAAATCTTACACCAGAAGAA+AGG 0.535334 8:-66000857 MsG0880045887.01.T01:CDS
GAAACCAACGTTCGAAAGAA+GGG 0.538266 8:+66001138 None:intergenic
ATTGCAGTTAGAAATGGTGT+TGG 0.538305 8:-66000510 MsG0880045887.01.T01:CDS
TTAGACAGTGATAGCATCCA+TGG 0.538602 8:+66000371 None:intergenic
GATTCACTCAACACCATGGC+AGG 0.542716 8:-66001725 None:intergenic
AATTATCGATTTAGCACCAT+GGG 0.552815 8:-66001636 MsG0880045887.01.T01:CDS
ACCAGCGTTGTTGCAAATGA+TGG 0.553843 8:+66001439 None:intergenic
GTTACTTGTACTGCAAGTAA+TGG 0.555096 8:+66001287 None:intergenic
CATACAGTTTCCTTTCACAA+AGG 0.556469 8:-66001096 MsG0880045887.01.T01:CDS
ATACTTTGGTTGAAGGATGT+CGG 0.566713 8:+66001376 None:intergenic
TGGGATTGGTAAAATTGCAA+TGG 0.567200 8:-66000709 MsG0880045887.01.T01:CDS
ATTCTTGTCCTACAATCTAC+GGG 0.570480 8:+66000819 None:intergenic
AACGAAGTGCAAAACTATGA+AGG 0.575652 8:-66001320 MsG0880045887.01.T01:CDS
GTGCTTGGTGGTGCAGAAGA+AGG 0.576706 8:-66000459 MsG0880045887.01.T01:CDS
AAAGCTAATGCAGAAATTGG+TGG 0.576763 8:-66000966 MsG0880045887.01.T01:CDS
TGGTTGAAGGATGTCGGCAA+TGG 0.578913 8:+66001382 None:intergenic
TCCATCATTTGCAACAACGC+TGG 0.580870 8:-66001440 MsG0880045887.01.T01:CDS
TTCGTTGAGTAAGAATAATG+AGG 0.583239 8:+66001337 None:intergenic
CTACCTGGTTCCGATATAGA+CGG 0.584378 8:+66000612 None:intergenic
TGGAAAGAAGTTGAGAGTGT+TGG 0.586632 8:+66001520 None:intergenic
TTTGTGAAAGGAAACTGTAT+GGG 0.590779 8:+66001098 None:intergenic
GGGAAATGATCATCTCTCCA+TGG 0.596430 8:-66000388 MsG0880045887.01.T01:CDS
ACAATCCGAGCAACAAAACA+TGG 0.599813 8:-66001680 MsG0880045887.01.T01:CDS
AAATTACTTGATGATCTACC+TGG 0.604805 8:+66000597 None:intergenic
GATAAGTAGAATGCCTGCCA+TGG 0.611905 8:+66001712 None:intergenic
ACCCCGTCTATATCGGAACC+AGG 0.621903 8:-66000615 MsG0880045887.01.T01:CDS
TAGTGTGTTCAGCTGTGGCA+TGG 0.623030 8:+66001405 None:intergenic
TCTTGGGCTGAAATCGCACA+AGG 0.627018 8:-66001182 MsG0880045887.01.T01:CDS
AATAATGAGGAAACAATAGG+AGG 0.628636 8:+66001350 None:intergenic
ACAAGACGACCAGCGAGTGG+TGG 0.630223 8:+66001500 None:intergenic
AATTGCAATGGAAATGAACA+AGG 0.638167 8:-66000697 MsG0880045887.01.T01:CDS
AAATTATCGATTTAGCACCA+TGG 0.647404 8:-66001637 MsG0880045887.01.T01:CDS
ATTTGGCGTCTACACCCACA+TGG 0.667304 8:+66001225 None:intergenic
AATGGTAGTGTGTTCAGCTG+TGG 0.672501 8:+66001400 None:intergenic
CAAGTAACAGAGTTAATCGA+CGG 0.676652 8:-66001272 MsG0880045887.01.T01:CDS
ACCTGGTTCCGATATAGACG+GGG 0.680926 8:+66000614 None:intergenic
ATTACAAGACGACCAGCGAG+TGG 0.695572 8:+66001497 None:intergenic
GTTGCAAATGATGGAACATG+AGG 0.705156 8:+66001448 None:intergenic
TGATAGCAAGCAGTTCACCA+CGG 0.707603 8:-66000572 MsG0880045887.01.T01:CDS
TTACCGTAATAATTGAACCG+TGG 0.738497 8:+66000555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTCTCTTCTAACTT+TGG + Chr8:66001292-66001311 None:intergenic 20.0%
!! GTTGGATTTAAAAGAAATAT+TGG - Chr8:66001589-66001608 MsG0880045887.01.T01:CDS 20.0%
!!! AAGTTAGAAGAGAATTTTTT+TGG - Chr8:66001292-66001311 MsG0880045887.01.T01:CDS 20.0%
! AAGAATAATGAGGAAACAAT+AGG + Chr8:66000737-66000756 None:intergenic 25.0%
! AATTATTACGGTAATGACTT+TGG - Chr8:66001535-66001554 MsG0880045887.01.T01:CDS 25.0%
! ATCAAAATCTCATCATGAAA+AGG - Chr8:66001414-66001433 MsG0880045887.01.T01:CDS 25.0%
! GATATGTAATAATGCAAGTA+GGG - Chr8:66001335-66001354 MsG0880045887.01.T01:CDS 25.0%
! GGATCTCAAATATCTTAAAA+TGG - Chr8:66000466-66000485 MsG0880045887.01.T01:CDS 25.0%
! TACTTATAATTAAACGCCAA+AGG + Chr8:66001201-66001220 None:intergenic 25.0%
!! TTGTCTTCCTTATGATATTT+TGG - Chr8:66001663-66001682 MsG0880045887.01.T01:CDS 25.0%
!!! AATTTTTTTGGAAGTGGAAT+CGG - Chr8:66001304-66001323 MsG0880045887.01.T01:CDS 25.0%
AAAATCTTACACCAGAAGAA+AGG - Chr8:66001224-66001243 MsG0880045887.01.T01:CDS 30.0%
AAATTAATGGAAAGTGGGAT+TGG - Chr8:66001358-66001377 MsG0880045887.01.T01:CDS 30.0%
AATAATGAGGAAACAATAGG+AGG + Chr8:66000734-66000753 None:intergenic 30.0%
AATGCAAGTAGGGAAATTAA+TGG - Chr8:66001345-66001364 MsG0880045887.01.T01:CDS 30.0%
AATTGCAATGGAAATGAACA+AGG - Chr8:66001384-66001403 MsG0880045887.01.T01:CDS 30.0%
ATAGCCTCCAAAATATCATA+AGG + Chr8:66001673-66001692 None:intergenic 30.0%
GATGAAATGCCAAAATGATT+TGG + Chr8:66000876-66000895 None:intergenic 30.0%
GGATATGTAATAATGCAAGT+AGG - Chr8:66001334-66001353 MsG0880045887.01.T01:CDS 30.0%
TAGGGAAATTAATGGAAAGT+GGG - Chr8:66001353-66001372 MsG0880045887.01.T01:CDS 30.0%
TAGTGAGTAATTATGAGTCT+TGG - Chr8:66001437-66001456 MsG0880045887.01.T01:CDS 30.0%
TCAAAAGCTAATGCAGAAAT+TGG - Chr8:66001112-66001131 MsG0880045887.01.T01:CDS 30.0%
TTCGTTGAGTAAGAATAATG+AGG + Chr8:66000747-66000766 None:intergenic 30.0%
TTTGTGAAAGGAAACTGTAT+GGG + Chr8:66000986-66001005 None:intergenic 30.0%
! AAAAGAAGACCTTTTTGGAT+TGG + Chr8:66000494-66000513 None:intergenic 30.0%
! AAATTACTTGATGATCTACC+TGG + Chr8:66001487-66001506 None:intergenic 30.0%
! GAATTTGTTCCTTAGTAAAG+TGG + Chr8:66001081-66001100 None:intergenic 30.0%
! TATGGAAAAGAAGACCTTTT+TGG + Chr8:66000499-66000518 None:intergenic 30.0%
! TTAAGATATTTGAGATCCCA+TGG + Chr8:66000464-66000483 None:intergenic 30.0%
! TTTGTTTAAGATGGTCGATT+TGG + Chr8:66000535-66000554 None:intergenic 30.0%
! TTTTGTGCTTCATTTGTGAA+AGG + Chr8:66001025-66001044 None:intergenic 30.0%
!! AAATTATCGATTTAGCACCA+TGG - Chr8:66000444-66000463 MsG0880045887.01.T01:CDS 30.0%
!! AATTATCGATTTAGCACCAT+GGG - Chr8:66000445-66000464 MsG0880045887.01.T01:CDS 30.0%
!! TATGATATTTTGGAGGCTAT+GGG - Chr8:66001673-66001692 MsG0880045887.01.T01:CDS 30.0%
!! TCTTCCTTATGATATTTTGG+AGG - Chr8:66001666-66001685 MsG0880045887.01.T01:CDS 30.0%
!! TGGAAAGTGTTTGTTTAAGA+TGG + Chr8:66000544-66000563 None:intergenic 30.0%
!! TTATGATATTTTGGAGGCTA+TGG - Chr8:66001672-66001691 MsG0880045887.01.T01:CDS 30.0%
!!! GAAGAGAATTTTTTTGGAAG+TGG - Chr8:66001298-66001317 MsG0880045887.01.T01:CDS 30.0%
!!! TTTGGTTTGTTTTGGGTTTA+TGG + Chr8:66000517-66000536 None:intergenic 30.0%
AAAATGGCACCAATCCAAAA+AGG - Chr8:66000482-66000501 MsG0880045887.01.T01:CDS 35.0%
AAAGCTAATGCAGAAATTGG+TGG - Chr8:66001115-66001134 MsG0880045887.01.T01:CDS 35.0%
AACGAAGTGCAAAACTATGA+AGG - Chr8:66000761-66000780 MsG0880045887.01.T01:CDS 35.0%
AAGCCTATTGCAGTTAGAAA+TGG - Chr8:66001565-66001584 MsG0880045887.01.T01:CDS 35.0%
ACAATAGGAGGAACATACTT+TGG + Chr8:66000722-66000741 None:intergenic 35.0%
ACACCATTTCTAACTGCAAT+AGG + Chr8:66001571-66001590 None:intergenic 35.0%
ATACTTTGGTTGAAGGATGT+CGG + Chr8:66000708-66000727 None:intergenic 35.0%
ATGGGACGATTAATGTTGTT+TGG + Chr8:66000968-66000987 None:intergenic 35.0%
ATTACGGTAATGACTTTGGT+TGG - Chr8:66001539-66001558 MsG0880045887.01.T01:CDS 35.0%
ATTCTTGTCCTACAATCTAC+GGG + Chr8:66001265-66001284 None:intergenic 35.0%
ATTGCAGTTAGAAATGGTGT+TGG - Chr8:66001571-66001590 MsG0880045887.01.T01:CDS 35.0%
ATTGGTAGAGTTATTGTGCT+TGG - Chr8:66001607-66001626 MsG0880045887.01.T01:CDS 35.0%
CAAGTAACAGAGTTAATCGA+CGG - Chr8:66000809-66000828 MsG0880045887.01.T01:CDS 35.0%
CATACAGTTTCCTTTCACAA+AGG - Chr8:66000985-66001004 MsG0880045887.01.T01:CDS 35.0%
CATTCTTGTCCTACAATCTA+CGG + Chr8:66001266-66001285 None:intergenic 35.0%
CTTTGTGAAAGGAAACTGTA+TGG + Chr8:66000987-66001006 None:intergenic 35.0%
GTAGGGAAATTAATGGAAAG+TGG - Chr8:66001352-66001371 MsG0880045887.01.T01:CDS 35.0%
GTTACTTGTACTGCAAGTAA+TGG + Chr8:66000797-66000816 None:intergenic 35.0%
TCACCACGGTTCAATTATTA+CGG - Chr8:66001523-66001542 MsG0880045887.01.T01:CDS 35.0%
TCGAATCTTCCACTTTACTA+AGG - Chr8:66001069-66001088 MsG0880045887.01.T01:CDS 35.0%
TTAATCGACGGTATCTTCAT+TGG - Chr8:66000821-66000840 MsG0880045887.01.T01:CDS 35.0%
TTACCGTAATAATTGAACCG+TGG + Chr8:66001529-66001548 None:intergenic 35.0%
TTACGGTAATGACTTTGGTT+GGG - Chr8:66001540-66001559 MsG0880045887.01.T01:CDS 35.0%
TTGGCATTTCATCAACTCTT+GGG - Chr8:66000883-66000902 MsG0880045887.01.T01:CDS 35.0%
TTTGGCATTTCATCAACTCT+TGG - Chr8:66000882-66000901 MsG0880045887.01.T01:CDS 35.0%
! GGTTTTGCAATTAACCATGT+GGG - Chr8:66000842-66000861 MsG0880045887.01.T01:CDS 35.0%
! TCGAAAGAAGGGATTTTTGT+TGG + Chr8:66000935-66000954 None:intergenic 35.0%
! TGGGATTGGTAAAATTGCAA+TGG - Chr8:66001372-66001391 MsG0880045887.01.T01:CDS 35.0%
! TTTTGTGCTTCCTTTGTGAA+AGG + Chr8:66000998-66001017 None:intergenic 35.0%
!! AAGCACTTTTAAGTCACCTT+TGG - Chr8:66001182-66001201 MsG0880045887.01.T01:CDS 35.0%
!! TGGTTTTGCAATTAACCATG+TGG - Chr8:66000841-66000860 MsG0880045887.01.T01:CDS 35.0%
!!! ATGGTCGATTTGGTTTGTTT+TGG + Chr8:66000525-66000544 None:intergenic 35.0%
!!! TGGTCGATTTGGTTTGTTTT+GGG + Chr8:66000524-66000543 None:intergenic 35.0%
AAATCCCTTCTTTCGAACGT+TGG - Chr8:66000939-66000958 MsG0880045887.01.T01:CDS 40.0%
ACAATCCGAGCAACAAAACA+TGG - Chr8:66000401-66000420 MsG0880045887.01.T01:CDS 40.0%
GAAACCAACGTTCGAAAGAA+GGG + Chr8:66000946-66000965 None:intergenic 40.0%
GAGGAACATACTTTGGTTGA+AGG + Chr8:66000715-66000734 None:intergenic 40.0%
GTTGAAAACCCCGTCTATAT+CGG - Chr8:66001459-66001478 MsG0880045887.01.T01:CDS 40.0%
TACGGTAATGACTTTGGTTG+GGG - Chr8:66001541-66001560 MsG0880045887.01.T01:CDS 40.0%
TTAGACAGTGATAGCATCCA+TGG + Chr8:66001713-66001732 None:intergenic 40.0%
! GTGTAGACGCCAAATCATTT+TGG - Chr8:66000864-66000883 MsG0880045887.01.T01:CDS 40.0%
! GTTGCAAATGATGGAACATG+AGG + Chr8:66000636-66000655 None:intergenic 40.0%
! TGGAAAGAAGTTGAGAGTGT+TGG + Chr8:66000564-66000583 None:intergenic 40.0%
ACCAGCGTTGTTGCAAATGA+TGG + Chr8:66000645-66000664 None:intergenic 45.0%
ACGAAAGCAGACCTTTCTTC+TGG + Chr8:66001238-66001257 None:intergenic 45.0%
CTACCTGGTTCCGATATAGA+CGG + Chr8:66001472-66001491 None:intergenic 45.0%
GGAAACCAACGTTCGAAAGA+AGG + Chr8:66000947-66000966 None:intergenic 45.0%
GGGAAATGATCATCTCTCCA+TGG - Chr8:66001693-66001712 MsG0880045887.01.T01:CDS 45.0%
GGTAGAGTTATTGTGCTTGG+TGG - Chr8:66001610-66001629 MsG0880045887.01.T01:CDS 45.0%
TACCTGGTTCCGATATAGAC+GGG + Chr8:66001471-66001490 None:intergenic 45.0%
TCCATCATTTGCAACAACGC+TGG - Chr8:66000641-66000660 MsG0880045887.01.T01:CDS 45.0%
TCGTGTTACCCGTAGATTGT+AGG - Chr8:66001254-66001273 MsG0880045887.01.T01:CDS 45.0%
TGATAGCAAGCAGTTCACCA+CGG - Chr8:66001509-66001528 MsG0880045887.01.T01:CDS 45.0%
! AATGGTAGTGTGTTCAGCTG+TGG + Chr8:66000684-66000703 None:intergenic 45.0%
!! ATGGAACATGAGGCGTTGTT+AGG + Chr8:66000626-66000645 None:intergenic 45.0%
!! CGTCACCATGTTTTGTTGCT+CGG + Chr8:66000409-66000428 None:intergenic 45.0%
AACTTCTTTCCACCACTCGC+TGG - Chr8:66000572-66000591 MsG0880045887.01.T01:CDS 50.0%
ACCTGGTTCCGATATAGACG+GGG + Chr8:66001470-66001489 None:intergenic 50.0%
ATTACAAGACGACCAGCGAG+TGG + Chr8:66000587-66000606 None:intergenic 50.0%
ATTTGGCGTCTACACCCACA+TGG + Chr8:66000859-66000878 None:intergenic 50.0%
TAGTGTGTTCAGCTGTGGCA+TGG + Chr8:66000679-66000698 None:intergenic 50.0%
TCTTGGGCTGAAATCGCACA+AGG - Chr8:66000899-66000918 MsG0880045887.01.T01:CDS 50.0%
TGGTTGAAGGATGTCGGCAA+TGG + Chr8:66000702-66000721 None:intergenic 50.0%
ACCCCGTCTATATCGGAACC+AGG - Chr8:66001466-66001485 MsG0880045887.01.T01:CDS 55.0%
GGAAGTGGAATCGGAGCAAC+TGG - Chr8:66001313-66001332 MsG0880045887.01.T01:CDS 55.0%
!! GTGCTTGGTGGTGCAGAAGA+AGG - Chr8:66001622-66001641 MsG0880045887.01.T01:CDS 55.0%
ACAAGACGACCAGCGAGTGG+TGG + Chr8:66000584-66000603 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 66000371 66001732 66000371 ID=MsG0880045887.01;Name=MsG0880045887.01
Chr8 mRNA 66000371 66001732 66000371 ID=MsG0880045887.01.T01;Parent=MsG0880045887.01;Name=MsG0880045887.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|453
Chr8 exon 66000371 66001732 66000371 ID=MsG0880045887.01.T01:exon:13715;Parent=MsG0880045887.01.T01
Chr8 CDS 66000371 66001732 66000371 ID=MsG0880045887.01.T01:cds;Parent=MsG0880045887.01.T01
Gene Sequence

>MsG0880045887.01.T01

ATGGCAGGCATTCTACTTATCTCGACTACTACAATCCGAGCAACAAAACATGGTGACGACAACTCAACTCACAAAATTATCGATTTAGCACCATGGGATCTCAAATATCTTAAAATGGCACCAATCCAAAAAGGTCTTCTTTTCCATAAACCCAAAACAAACCAAATCGACCATCTTAAACAAACACTTTCCAACACTCTCAACTTCTTTCCACCACTCGCTGGTCGTCTTGTAATAACACAACACGACGAACCTAACAACGCCTCATGTTCCATCATTTGCAACAACGCTGGTGCATTGTTTGTCCATGCCACAGCTGAACACACTACCATTGCCGACATCCTTCAACCAAAGTATGTTCCTCCTATTGTTTCCTCATTATTCTTACTCAACGAAGTGCAAAACTATGAAGGCACATCACAACCATTACTTGCAGTACAAGTAACAGAGTTAATCGACGGTATCTTCATTGGTTTTGCAATTAACCATGTGGGTGTAGACGCCAAATCATTTTGGCATTTCATCAACTCTTGGGCTGAAATCGCACAAGGTTTCAATAAGCCAACAAAAATCCCTTCTTTCGAACGTTGGTTTCCAAACAACATTAATCGTCCCATACAGTTTCCTTTCACAAAGGAAGCACAAAAGCAGCCTTTCACAAATGAAGCACAAAATCAACAATGTGAAGTTTCGTCTCATCGAATCTTCCACTTTACTAAGGAACAAATTCTGCAACTCAAATCAAAAGCTAATGCAGAAATTGGTGGTAGTAGTACTAGTAGTGATAAAATAATAACAATATCGTCATTGCAAGCACTTTTAAGTCACCTTTGGCGTTTAATTATAAGTAAACAAAATCTTACACCAGAAGAAAGGTCTGCTTTCGTGTTACCCGTAGATTGTAGGACAAGAATGTGTCCAAAGTTAGAAGAGAATTTTTTTGGAAGTGGAATCGGAGCAACTGGATATGTAATAATGCAAGTAGGGAAATTAATGGAAAGTGGGATTGGTAAAATTGCAATGGAAATGAACAAGGAGATTTCATCAAAATCTCATCATGAAAAGGTAGTGAGTAATTATGAGTCTTGGTTGAAAACCCCGTCTATATCGGAACCAGGTAGATCATCAAGTAATTTGTTGATAGCAAGCAGTTCACCACGGTTCAATTATTACGGTAATGACTTTGGTTGGGGTAAGCCTATTGCAGTTAGAAATGGTGTTGGATTTAAAAGAAATATTGGTAGAGTTATTGTGCTTGGTGGTGCAGAAGAAGGTAGTATTGATATTCAAGTTTGTCTTCCTTATGATATTTTGGAGGCTATGGGAAATGATCATCTCTCCATGGATGCTATCACTGTCTAA
Protein sequence

>MsG0880045887.01.T01

MAGILLISTTTIRATKHGDDNSTHKIIDLAPWDLKYLKMAPIQKGLLFHKPKTNQIDHLKQTLSNTLNFFPPLAGRLVITQHDEPNNASCSIICNNAGALFVHATAEHTTIADILQPKYVPPIVSSLFLLNEVQNYEGTSQPLLAVQVTELIDGIFIGFAINHVGVDAKSFWHFINSWAEIAQGFNKPTKIPSFERWFPNNINRPIQFPFTKEAQKQPFTNEAQNQQCEVSSHRIFHFTKEQILQLKSKANAEIGGSSTSSDKIITISSLQALLSHLWRLIISKQNLTPEERSAFVLPVDCRTRMCPKLEENFFGSGIGATGYVIMQVGKLMESGIGKIAMEMNKEISSKSHHEKVVSNYESWLKTPSISEPGRSSSNLLIASSSPRFNYYGNDFGWGKPIAVRNGVGFKRNIGRVIVLGGAEEGSIDIQVCLPYDILEAMGNDHLSMDAITV*