AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016378.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016378.01.T01 MTR_3g088420 94.919 433 22 0 1 433 1 433 0.0 863
MsG0380016378.01.T01 MTR_3g026310 72.545 448 108 3 1 433 1 448 0.0 662
MsG0380016378.01.T01 MTR_4g022800 69.005 442 128 3 1 433 1 442 0.0 617
MsG0380016378.01.T01 MTR_4g022670 67.574 441 135 2 1 433 1 441 0.0 613
MsG0380016378.01.T01 MTR_4g022825 67.727 440 135 1 1 433 1 440 0.0 609
MsG0380016378.01.T01 MTR_4g022720 68.506 435 135 2 1 433 1 435 0.0 605
MsG0380016378.01.T01 MTR_7g026860 67.120 441 134 3 1 433 1 438 0.0 591
MsG0380016378.01.T01 MTR_8g041770 61.937 444 155 6 1 433 1 441 0.0 539
MsG0380016378.01.T01 MTR_0190s0020 61.036 444 160 6 1 433 1 442 0.0 531
MsG0380016378.01.T01 MTR_4g022760 60.544 441 122 3 1 433 1 397 0.0 521
MsG0380016378.01.T01 MTR_4g094842 56.763 451 170 9 1 431 1 446 3.20e-169 483
MsG0380016378.01.T01 MTR_4g094835 54.464 448 179 10 1 429 1 442 1.71e-164 470
MsG0380016378.01.T01 MTR_4g094828 51.868 455 194 11 1 434 1 451 1.78e-153 442
MsG0380016378.01.T01 MTR_6g016815 55.196 433 128 6 1 433 15 381 5.97e-153 439
MsG0380016378.01.T01 MTR_8g079710 54.362 447 180 12 1 429 1 441 2.55e-150 434
MsG0380016378.01.T01 MTR_4g094838 51.670 449 173 9 4 429 1 428 4.39e-149 431
MsG0380016378.01.T01 MTR_4g022290 51.441 451 189 11 1 430 1 442 2.40e-146 424
MsG0380016378.01.T01 MTR_4g094832 55.053 376 148 7 69 429 57 426 3.33e-136 398
MsG0380016378.01.T01 MTR_3g101900 39.352 432 239 10 19 434 25 449 5.00e-94 290
MsG0380016378.01.T01 MTR_8g040780 63.229 223 78 2 210 428 5 227 2.96e-93 281
MsG0380016378.01.T01 MTR_8g040760 65.566 212 67 2 1 206 1 212 4.98e-91 275
MsG0380016378.01.T01 MTR_3g101930 37.762 429 243 9 24 432 30 454 2.00e-86 271
MsG0380016378.01.T01 MTR_3g102020 37.931 435 245 10 19 434 24 452 2.71e-86 271
MsG0380016378.01.T01 MTR_3g101970 37.188 441 239 11 11 434 19 438 7.04e-84 264
MsG0380016378.01.T01 MTR_3g102010 37.995 429 242 10 24 434 30 452 1.78e-83 263
MsG0380016378.01.T01 MTR_3g101960 36.574 432 242 11 19 428 24 445 3.70e-80 255
MsG0380016378.01.T01 MTR_7g026850 72.903 155 42 0 29 183 12 166 6.46e-79 242
MsG0380016378.01.T01 MTR_3g070170 31.381 478 265 13 2 433 10 470 1.36e-62 209
MsG0380016378.01.T01 MTR_6g034485 72.581 124 33 1 189 312 3 125 1.66e-58 188
MsG0380016378.01.T01 MTR_7g026770 49.787 235 76 4 109 322 80 293 2.10e-58 194
MsG0380016378.01.T01 MTR_5g081960 31.381 478 249 12 19 429 17 482 2.57e-58 199
MsG0380016378.01.T01 MTR_4g015180 32.985 479 242 16 20 433 23 487 1.74e-56 194
MsG0380016378.01.T01 MTR_4g094825 56.287 167 66 4 16 176 83 248 5.88e-53 182
MsG0380016378.01.T01 MTR_4g094825 51.656 151 60 2 285 433 252 391 1.10e-41 152
MsG0380016378.01.T01 MTR_6g045553 29.957 464 281 12 4 429 14 471 2.54e-50 177
MsG0380016378.01.T01 MTR_6g034495 62.774 137 41 3 49 180 33 164 1.00e-47 161
MsG0380016378.01.T01 MTR_3g102030 33.550 307 170 6 87 389 44 320 1.34e-45 160
MsG0380016378.01.T01 MTR_7g026830 75.000 84 21 0 82 165 14 97 1.86e-39 137
MsG0380016378.01.T01 MTR_8g096530 28.261 414 228 15 53 426 57 441 6.26e-32 126
MsG0380016378.01.T01 MTR_3g026950 27.596 366 221 12 52 385 51 404 1.57e-30 122
MsG0380016378.01.T01 MTR_7g026800 45.000 160 57 4 215 372 30 160 3.11e-29 113
MsG0380016378.01.T01 MTR_4g007540 24.490 441 284 15 4 417 3 421 6.47e-29 118
MsG0380016378.01.T01 MTR_8g075610 26.943 386 245 15 55 422 47 413 1.29e-28 117
MsG0380016378.01.T01 MTR_3g098980 27.066 351 209 11 55 385 66 389 1.41e-28 117
MsG0380016378.01.T01 MTR_4g022780 43.558 163 61 4 214 374 30 163 5.81e-28 109
MsG0380016378.01.T01 MTR_2g105330 25.594 379 248 12 55 415 56 418 1.19e-27 114
MsG0380016378.01.T01 MTR_2g064495 25.654 382 244 13 55 415 56 418 9.35e-27 112
MsG0380016378.01.T01 MTR_7g026780 50.909 110 30 2 324 433 26 111 2.53e-26 102
MsG0380016378.01.T01 MTR_3g049150 28.684 380 222 17 42 384 32 399 3.91e-26 110
MsG0380016378.01.T01 MTR_4g013050 25.419 358 205 9 49 385 47 363 2.67e-24 104
MsG0380016378.01.T01 MTR_4g097090 25.000 448 283 19 21 432 27 457 2.58e-22 99.4
MsG0380016378.01.T01 MTR_4g127650 25.362 414 264 15 49 432 59 457 2.60e-22 99.4
MsG0380016378.01.T01 MTR_1g081660 25.773 388 240 15 24 385 31 396 7.35e-21 94.7
MsG0380016378.01.T01 MTR_7g085930 26.166 386 218 13 52 390 56 421 4.66e-20 92.4
MsG0380016378.01.T01 MTR_2g089670 26.601 406 238 15 21 384 42 429 2.68e-19 90.5
MsG0380016378.01.T01 MTR_7g014330 24.096 415 248 15 19 388 21 413 3.03e-19 90.1
MsG0380016378.01.T01 MTR_7g027175 24.619 394 266 12 50 431 63 437 4.09e-19 89.4
MsG0380016378.01.T01 MTR_6g015830 24.208 442 287 12 15 422 25 452 2.85e-18 87.0
MsG0380016378.01.T01 MTR_7g026870 48.718 78 40 0 266 343 74 151 3.21e-18 81.6
MsG0380016378.01.T01 MTR_1g040195 26.603 421 260 18 25 427 23 412 3.60e-18 86.7
MsG0380016378.01.T01 MTR_6g015320 24.808 391 257 11 24 387 29 409 3.78e-18 86.7
MsG0380016378.01.T01 MTR_5g076700 23.303 442 278 17 25 429 24 441 6.21e-18 86.3
MsG0380016378.01.T01 MTR_2g089755 25.346 434 260 19 24 409 37 454 7.51e-18 85.9
MsG0380016378.01.T01 MTR_6g015850 23.884 448 286 14 8 421 22 448 1.19e-17 85.1
MsG0380016378.01.T01 MTR_7g085990 26.471 306 199 8 52 337 52 351 6.29e-17 83.2
MsG0380016378.01.T01 MTR_7g026880 63.462 52 19 0 382 433 2 53 6.63e-17 74.7
MsG0380016378.01.T01 MTR_3g087640 25.282 443 278 16 22 420 29 462 5.77e-16 80.1
MsG0380016378.01.T01 MTR_7g114470 23.799 437 283 15 25 429 25 443 6.40e-16 80.1
MsG0380016378.01.T01 MTR_7g014200 24.146 410 255 14 22 388 28 424 1.95e-15 78.6
MsG0380016378.01.T01 MTR_2g089650 23.709 426 245 16 38 409 43 442 2.66e-15 78.2
MsG0380016378.01.T01 MTR_7g114550 21.622 444 282 19 25 429 24 440 2.96e-15 77.8
MsG0380016378.01.T01 MTR_7g014620 24.942 429 255 17 24 414 15 414 3.27e-15 77.8
MsG0380016378.01.T01 MTR_8g012750 24.816 407 254 12 22 384 24 422 4.07e-15 77.4
MsG0380016378.01.T01 MTR_3g086020 25.620 363 223 11 55 406 61 387 4.91e-15 77.0
MsG0380016378.01.T01 MTR_7g015860 25.914 301 170 11 122 385 129 413 5.37e-15 77.0
MsG0380016378.01.T01 MTR_7g084910 28.467 274 173 9 49 308 62 326 7.44e-15 76.6
MsG0380016378.01.T01 MTR_1g040175 27.305 282 175 11 50 317 59 324 1.09e-14 76.3
MsG0380016378.01.T01 MTR_3g028210 22.511 462 288 17 4 415 8 449 1.11e-14 76.3
MsG0380016378.01.T01 MTR_1g053315 25.323 387 247 19 55 416 78 447 1.37e-14 75.9
MsG0380016378.01.T01 MTR_7g086000 30.385 260 159 12 58 302 63 315 1.39e-14 75.9
MsG0380016378.01.T01 MTR_2g089765 23.697 422 255 14 15 384 28 434 1.53e-14 75.9
MsG0380016378.01.T01 MTR_7g114520 24.365 394 239 19 25 385 25 392 1.68e-14 75.5
MsG0380016378.01.T01 MTR_7g084940 28.223 287 172 11 49 316 61 332 3.09e-14 74.7
MsG0380016378.01.T01 MTR_4g055170 25.413 303 196 10 55 343 69 355 1.16e-13 73.2
MsG0380016378.01.T01 MTR_0502s0010 28.879 232 149 9 95 317 1 225 1.74e-13 70.1
MsG0380016378.01.T01 MTR_3g465980 25.993 277 177 8 55 316 68 331 4.29e-13 71.2
MsG0380016378.01.T01 MTR_5g090880 24.213 413 244 17 55 422 67 455 5.06e-13 71.2
MsG0380016378.01.T01 MTR_5g090810 24.623 398 235 17 32 385 36 412 5.50e-13 70.9
MsG0380016378.01.T01 MTR_3g049140 25.974 231 147 9 204 414 6 232 1.43e-12 67.8
MsG0380016378.01.T01 MTR_5g090830 24.433 397 237 16 32 385 36 412 1.65e-12 69.3
MsG0380016378.01.T01 MTR_3g112180 24.041 391 253 16 52 420 58 426 9.92e-12 67.0
MsG0380016378.01.T01 MTR_7g014360 25.856 263 174 10 138 388 90 343 1.03e-11 66.6
MsG0380016378.01.T01 MTR_3g465990 25.725 276 179 10 55 316 68 331 1.91e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016378.01.T01 AT5G67160 40.183 438 237 11 6 434 12 433 3.48e-98 301
MsG0380016378.01.T01 AT5G67150 38.767 454 251 11 1 434 1 447 1.25e-97 300
MsG0380016378.01.T01 AT3G50300 42.290 428 229 11 1 414 1 424 4.57e-97 298
MsG0380016378.01.T01 AT3G50280 38.202 445 255 11 1 430 1 440 1.08e-92 287
MsG0380016378.01.T01 AT3G50270 39.450 436 248 11 12 434 17 449 3.48e-91 283
MsG0380016378.01.T01 AT5G38130 40.233 430 233 12 1 414 21 442 4.32e-88 276
MsG0380016378.01.T01 AT5G42830 36.866 434 256 10 15 434 21 450 5.18e-85 267
MsG0380016378.01.T01 AT5G07860 36.807 451 257 14 2 432 6 448 3.14e-78 250
MsG0380016378.01.T01 AT5G07870 36.364 462 258 14 2 433 6 461 6.11e-77 247
MsG0380016378.01.T01 AT5G07850 35.153 458 261 15 2 433 6 453 3.75e-70 229
MsG0380016378.01.T01 AT2G39980 32.712 483 254 13 11 433 9 480 8.77e-66 218
MsG0380016378.01.T01 AT3G50280 38.782 312 179 6 127 430 3 310 2.85e-65 212
MsG0380016378.01.T01 AT3G50290 42.593 270 142 5 122 382 4 269 2.63e-61 200
MsG0380016378.01.T01 AT5G01210 32.439 410 222 11 66 433 73 469 9.42e-59 199
MsG0380016378.01.T01 AT5G23940 31.648 455 273 15 4 429 3 448 1.96e-56 194
MsG0380016378.01.T01 AT3G48720 27.907 344 216 11 55 385 61 385 1.33e-27 114
MsG0380016378.01.T01 AT5G48930 27.348 362 218 15 53 391 54 393 3.80e-27 113
MsG0380016378.01.T01 AT5G41040 26.225 347 226 10 52 385 66 395 1.80e-25 108
MsG0380016378.01.T01 AT5G41040 26.801 347 224 11 52 385 82 411 1.93e-25 108
MsG0380016378.01.T01 AT2G19070 26.014 419 253 19 50 431 53 451 1.06e-22 100
MsG0380016378.01.T01 AT5G57840 28.030 396 231 18 49 414 51 422 1.79e-22 99.8
MsG0380016378.01.T01 AT3G29590 24.499 449 301 12 2 424 6 442 3.02e-22 99.0
MsG0380016378.01.T01 AT3G29635 25.952 420 272 14 24 414 29 438 7.01e-21 95.1
MsG0380016378.01.T01 AT3G26040 26.290 407 246 17 51 434 55 430 9.82e-21 94.4
MsG0380016378.01.T01 AT5G63560 25.501 349 214 12 55 385 61 381 2.03e-20 93.2
MsG0380016378.01.T01 AT1G28680 24.390 451 285 18 1 427 1 419 3.32e-19 90.1
MsG0380016378.01.T01 AT3G29670 22.706 436 285 15 20 422 25 441 1.78e-18 87.8
MsG0380016378.01.T01 AT5G39050 26.836 354 187 14 122 420 132 468 7.61e-18 85.9
MsG0380016378.01.T01 AT1G24420 23.556 450 301 16 4 434 5 430 8.83e-18 85.5
MsG0380016378.01.T01 AT1G24420 23.556 450 301 16 4 434 5 430 8.83e-18 85.5
MsG0380016378.01.T01 AT2G40230 27.136 398 243 17 11 389 22 391 1.10e-17 85.1
MsG0380016378.01.T01 AT1G28680 24.932 365 230 16 1 343 1 343 1.96e-16 81.3
MsG0380016378.01.T01 AT5G39090 28.831 385 216 18 42 394 56 414 8.65e-16 79.7
MsG0380016378.01.T01 AT3G30280 22.963 405 271 16 49 434 55 437 2.04e-15 78.6
MsG0380016378.01.T01 AT1G24430 24.868 378 218 16 54 406 61 397 6.05e-15 77.0
MsG0380016378.01.T01 AT1G03390 23.587 407 257 16 42 415 59 444 2.18e-14 75.5
MsG0380016378.01.T01 AT5G23970 22.637 455 283 16 4 429 3 417 4.32e-14 74.3
MsG0380016378.01.T01 AT5G61160 22.251 391 267 13 22 385 23 403 5.01e-14 74.3
MsG0380016378.01.T01 AT3G29680 23.858 394 250 12 24 385 25 400 2.06e-13 72.4
MsG0380016378.01.T01 AT1G78990 25.706 354 220 15 53 385 65 396 2.70e-13 72.0
MsG0380016378.01.T01 AT5G47950 23.714 350 217 15 50 385 56 369 2.00e-12 68.9

Find 91 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCGTGGTTTGATGTTTA+TGG 0.126200 3:+82676838 MsG0380016378.01.T01:CDS
CCATTGCCTGAAGATTATTT+TGG 0.195668 3:+82676607 MsG0380016378.01.T01:CDS
ATTGGCTAATGTATTATTAT+TGG 0.203749 3:-82676809 None:intergenic
GTGTCTTCCTCACAAGATTT+TGG 0.229479 3:+82676969 MsG0380016378.01.T01:CDS
ACTAATTGCACCCACCAAAA+AGG 0.252147 3:+82675829 MsG0380016378.01.T01:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 3:+82676850 MsG0380016378.01.T01:CDS
GCCTGCAAGTGGTGGGAAAA+AGG 0.338259 3:-82675924 None:intergenic
ACGTTCTAGTGTTATGAGTT+TGG 0.352542 3:-82676287 None:intergenic
TTCAGATGTTGAATTTGATT+TGG 0.376205 3:-82675878 None:intergenic
GTGGGCTTGGTAAAGGTGCT+TGG 0.384077 3:+82676686 MsG0380016378.01.T01:CDS
ACACGAAGAAGTTTATTATA+TGG 0.390132 3:+82676552 MsG0380016378.01.T01:CDS
TTCGTGTGGGTGAAATTGTT+TGG 0.393408 3:-82676536 None:intergenic
TTTAACCACGCGGTTGTTGA+TGG 0.400033 3:+82676199 MsG0380016378.01.T01:CDS
GCATTTCCAAAATAATCTTC+AGG 0.400923 3:-82676613 None:intergenic
AATGAAAGCAGGACAGTTGT+TGG 0.413215 3:+82676657 MsG0380016378.01.T01:CDS
AATTTGGTGACTTGATTGTA+TGG 0.415203 3:-82675760 None:intergenic
GGAATGATTACTGTATTTGC+TGG 0.415451 3:+82676916 MsG0380016378.01.T01:CDS
TTGTATGGGTAGAGAGGACT+TGG 0.418563 3:-82675745 None:intergenic
AAACCACGGGGAATTGCTAT+TGG 0.430657 3:-82676827 None:intergenic
GGGAAATGACCATGACTTCA+TGG 0.444511 3:+82676999 MsG0380016378.01.T01:CDS
TGGAAAAGAAGAAGGTAGTA+TGG 0.447023 3:+82676936 MsG0380016378.01.T01:CDS
GAAATGCTGTTGAAGTTTGT+AGG 0.451612 3:+82676629 MsG0380016378.01.T01:CDS
TTAGTTGACGGCATCTTCAT+TGG 0.458413 3:+82676169 MsG0380016378.01.T01:CDS
TTGAAAGCCAACATGGAGAT+TGG 0.465372 3:+82676451 MsG0380016378.01.T01:CDS
CGCAGTGGAAATAAAATAAG+TGG 0.469980 3:+82676895 MsG0380016378.01.T01:CDS
TGAAGAATCACTACGAGTCT+TGG 0.474671 3:+82676749 MsG0380016378.01.T01:CDS
CCAAAATAATCTTCAGGCAA+TGG 0.482695 3:-82676607 None:intergenic
TGGGCTTGGTAAAGGTGCTT+GGG 0.485107 3:+82676687 MsG0380016378.01.T01:CDS
ATTTAACTCCATGGGATCTC+CGG 0.488953 3:+82675801 MsG0380016378.01.T01:CDS
GAAGATGGTGGGCTTGGTAA+AGG 0.490096 3:+82676679 MsG0380016378.01.T01:CDS
ACCTTGATCTAGCTCCTATC+GGG 0.496130 3:-82676576 None:intergenic
AACCTTGATCTAGCTCCTAT+CGG 0.500296 3:-82676577 None:intergenic
GCATGTGACGGAACATGAAA+TGG 0.505364 3:-82675978 None:intergenic
AATCACTACGAGTCTTGGTT+GGG 0.505413 3:+82676754 MsG0380016378.01.T01:CDS
GTTGGTACCAATCTCCATGT+TGG 0.507485 3:-82676458 None:intergenic
GAATCACTACGAGTCTTGGT+TGG 0.508104 3:+82676753 MsG0380016378.01.T01:CDS
TCGAGACGGCCTGCAAGTGG+TGG 0.521804 3:-82675932 None:intergenic
GCAGGACAGTTGTTGGAAGA+TGG 0.523872 3:+82676664 MsG0380016378.01.T01:CDS
TATAGTGAAAGGAAATCGTA+TGG 0.523888 3:-82676335 None:intergenic
AACTCATAACACTAGAACGT+TGG 0.524522 3:+82676290 MsG0380016378.01.T01:CDS
TACCATAAACATCAAACCAC+GGG 0.529973 3:-82676840 None:intergenic
TTTCCACTGCGAATTGCCAC+AGG 0.532396 3:-82676883 None:intergenic
GACAGTTGTTGGAAGATGGT+GGG 0.539492 3:+82676668 MsG0380016378.01.T01:CDS
TGTGATCTGTTATTTCGAGA+CGG 0.540547 3:-82675946 None:intergenic
AAATGGAATAACCTCTCTGA+TGG 0.546561 3:-82676400 None:intergenic
ACTTGCGTAGGTGACATACT+TGG 0.546620 3:+82676043 MsG0380016378.01.T01:CDS
TGTAGGGTTACAATGAAAGC+AGG 0.550470 3:+82676646 MsG0380016378.01.T01:CDS
TTGTTGGAAGATGGTGGGCT+TGG 0.553654 3:+82676673 MsG0380016378.01.T01:CDS
ACCAGCATTGTTGCATGTGA+CGG 0.553807 3:-82675990 None:intergenic
AAATGCTGTTGAAGTTTGTA+GGG 0.553867 3:+82676630 MsG0380016378.01.T01:CDS
TCCCGATAGGAGCTAGATCA+AGG 0.559796 3:+82676575 MsG0380016378.01.T01:CDS
GTATTTGCTGGAAAAGAAGA+AGG 0.567059 3:+82676928 MsG0380016378.01.T01:CDS
AAATAATCTTCAGGCAATGG+TGG 0.571644 3:-82676604 None:intergenic
GGACAGTTGTTGGAAGATGG+TGG 0.574775 3:+82676667 MsG0380016378.01.T01:CDS
AACGGATCACAGAGCGCCAT+AGG 0.575889 3:-82676513 None:intergenic
ACTGAAATTGAAAGCCAACA+TGG 0.579416 3:+82676444 MsG0380016378.01.T01:CDS
ATTTCGAGACGGCCTGCAAG+TGG 0.589823 3:-82675935 None:intergenic
TGGGTGCAATTAGTAGGTAC+CGG 0.591093 3:-82675820 None:intergenic
GTTTATTATATGGTCCCGAT+AGG 0.594388 3:+82676562 MsG0380016378.01.T01:CDS
AAGTGTTCTCCGCTGCGGCA+TGG 0.595760 3:-82676024 None:intergenic
ATATAATAAACTTCTTCGTG+TGG 0.595960 3:-82676550 None:intergenic
TGAATTTGATTTGGCACTAG+AGG 0.597459 3:-82675869 None:intergenic
TATAATAAACTTCTTCGTGT+GGG 0.598129 3:-82676549 None:intergenic
TCCGTCACATGCAACAATGC+TGG 0.599979 3:+82675989 MsG0380016378.01.T01:CDS
ACACAACATCCATGAAGTCA+TGG 0.605152 3:-82677008 None:intergenic
ATCGACGATGGGAGGAACGT+AGG 0.605346 3:-82676071 None:intergenic
AGTAGGTACCGGAGATCCCA+TGG 0.606618 3:-82675809 None:intergenic
CGAGACGGCCTGCAAGTGGT+GGG 0.611766 3:-82675931 None:intergenic
ATTTGGTGACTTGATTGTAT+GGG 0.612690 3:-82675759 None:intergenic
ATGGGATGTTGAATACCACT+TGG 0.613611 3:-82676316 None:intergenic
GTGGGTGAAATTGTTTGGAA+CGG 0.621788 3:-82676531 None:intergenic
CAAGTGACTGAGTTAGTTGA+CGG 0.622653 3:+82676157 MsG0380016378.01.T01:CDS
TTTGATTTGGCACTAGAGGA+TGG 0.624229 3:-82675865 None:intergenic
ATAGTGAAAGGAAATCGTAT+GGG 0.628856 3:-82676334 None:intergenic
ACTTGATTGTATGGGTAGAG+AGG 0.629120 3:-82675751 None:intergenic
TTGTTGATGGAGAGTCCACA+TGG 0.630682 3:+82676212 MsG0380016378.01.T01:CDS
GTCACTTGCACAGCTAACAA+TGG 0.634835 3:-82676142 None:intergenic
GAGGTTATTCCATTTCACAA+AGG 0.635344 3:+82676408 MsG0380016378.01.T01:CDS
TCATGGGCTAAAATCTCGCG+TGG 0.637561 3:+82676247 MsG0380016378.01.T01:CDS
AAACCTGTGGCAATTCGCAG+TGG 0.640198 3:+82676880 MsG0380016378.01.T01:CDS
TTACCATAAACATCAAACCA+CGG 0.662908 3:-82676841 None:intergenic
ATTGCCTCCAAAATCTTGTG+AGG 0.672285 3:-82676976 None:intergenic
GCAGCGGAGAACACTTGCGT+AGG 0.674294 3:+82676031 MsG0380016378.01.T01:CDS
ATCGGAGTTCAAAACTACGA+AGG 0.674883 3:+82676109 MsG0380016378.01.T01:CDS
CTAGAACGTTGGTTTCCAAG+TGG 0.676278 3:+82676301 MsG0380016378.01.T01:CDS
TACGCAAGTGTTCTCCGCTG+CGG 0.687317 3:-82676029 None:intergenic
TGAGTTAATGAAAAGCCATG+TGG 0.717706 3:-82676227 None:intergenic
GCTCTTTGTCCATGCCGCAG+CGG 0.721004 3:+82676015 MsG0380016378.01.T01:CDS
ACCATAAACATCAAACCACG+GGG 0.743607 3:-82676839 None:intergenic
TAGCCAATAGCAATTCCCCG+TGG 0.764549 3:+82676824 MsG0380016378.01.T01:CDS
ACTCTCCATCAACAACCGCG+TGG 0.786602 3:-82676204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTGATCAATTGAATT+TGG - Chr3:82675779-82675798 None:intergenic 15.0%
!! ATTGGCTAATGTATTATTAT+TGG - Chr3:82676812-82676831 None:intergenic 20.0%
!! CTAAAAAATCTAACAATGTT+TGG - Chr3:82676784-82676803 None:intergenic 20.0%
!!! AAAAAATTGATTTAACTCCA+TGG + Chr3:82675792-82675811 MsG0380016378.01.T01:CDS 20.0%
!!! AAAAATTGATTTAACTCCAT+GGG + Chr3:82675793-82675812 MsG0380016378.01.T01:CDS 20.0%
!!! GATTCATTTTTTCCATTAAT+CGG + Chr3:82676091-82676110 MsG0380016378.01.T01:CDS 20.0%
! ACACGAAGAAGTTTATTATA+TGG + Chr3:82676552-82676571 MsG0380016378.01.T01:CDS 25.0%
! ATATAATAAACTTCTTCGTG+TGG - Chr3:82676553-82676572 None:intergenic 25.0%
! TATAATAAACTTCTTCGTGT+GGG - Chr3:82676552-82676571 None:intergenic 25.0%
! TTCAGATGTTGAATTTGATT+TGG - Chr3:82675881-82675900 None:intergenic 25.0%
!!! GATGTTTATGGTAATGATTT+TGG + Chr3:82676850-82676869 MsG0380016378.01.T01:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG + Chr3:82676855-82676874 MsG0380016378.01.T01:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG + Chr3:82676854-82676873 MsG0380016378.01.T01:CDS 25.0%
AAAAATTCAATCCATCAGAG+AGG + Chr3:82676389-82676408 MsG0380016378.01.T01:CDS 30.0%
AAATGCTGTTGAAGTTTGTA+GGG + Chr3:82676630-82676649 MsG0380016378.01.T01:CDS 30.0%
ATAGTGAAAGGAAATCGTAT+GGG - Chr3:82676337-82676356 None:intergenic 30.0%
GCATTTCCAAAATAATCTTC+AGG - Chr3:82676616-82676635 None:intergenic 30.0%
TATAGTGAAAGGAAATCGTA+TGG - Chr3:82676338-82676357 None:intergenic 30.0%
TTACCATAAACATCAAACCA+CGG - Chr3:82676844-82676863 None:intergenic 30.0%
! AATTTGGTGACTTGATTGTA+TGG - Chr3:82675763-82675782 None:intergenic 30.0%
! ATGGCTTTTCATTAACTCAT+GGG + Chr3:82676231-82676250 MsG0380016378.01.T01:CDS 30.0%
! ATTTGGTGACTTGATTGTAT+GGG - Chr3:82675762-82675781 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG + Chr3:82676856-82676875 MsG0380016378.01.T01:CDS 30.0%
!!! TAGTTTTGAACTCCGATTAA+TGG - Chr3:82676106-82676125 None:intergenic 30.0%
AAATAATCTTCAGGCAATGG+TGG - Chr3:82676607-82676626 None:intergenic 35.0%
AAATGGAATAACCTCTCTGA+TGG - Chr3:82676403-82676422 None:intergenic 35.0%
AACTCATAACACTAGAACGT+TGG + Chr3:82676290-82676309 MsG0380016378.01.T01:CDS 35.0%
ACGTTCTAGTGTTATGAGTT+TGG - Chr3:82676290-82676309 None:intergenic 35.0%
ACTGAAATTGAAAGCCAACA+TGG + Chr3:82676444-82676463 MsG0380016378.01.T01:CDS 35.0%
CCAAAATAATCTTCAGGCAA+TGG - Chr3:82676610-82676629 None:intergenic 35.0%
CGCAGTGGAAATAAAATAAG+TGG + Chr3:82676895-82676914 MsG0380016378.01.T01:CDS 35.0%
GAAATGCTGTTGAAGTTTGT+AGG + Chr3:82676629-82676648 MsG0380016378.01.T01:CDS 35.0%
GAGGTTATTCCATTTCACAA+AGG + Chr3:82676408-82676427 MsG0380016378.01.T01:CDS 35.0%
GGAAAAAATGAATCGACGAT+GGG - Chr3:82676085-82676104 None:intergenic 35.0%
GGAATGATTACTGTATTTGC+TGG + Chr3:82676916-82676935 MsG0380016378.01.T01:CDS 35.0%
GTTTATTATATGGTCCCGAT+AGG + Chr3:82676562-82676581 MsG0380016378.01.T01:CDS 35.0%
TACCATAAACATCAAACCAC+GGG - Chr3:82676843-82676862 None:intergenic 35.0%
TGAGTTAATGAAAAGCCATG+TGG - Chr3:82676230-82676249 None:intergenic 35.0%
TGGAAAAAATGAATCGACGA+TGG - Chr3:82676086-82676105 None:intergenic 35.0%
TGGAAAAGAAGAAGGTAGTA+TGG + Chr3:82676936-82676955 MsG0380016378.01.T01:CDS 35.0%
! ATAAAGAAGACCCTTTTTGG+TGG - Chr3:82675843-82675862 None:intergenic 35.0%
! CATGGCTTTTCATTAACTCA+TGG + Chr3:82676230-82676249 MsG0380016378.01.T01:CDS 35.0%
! CCATTGCCTGAAGATTATTT+TGG + Chr3:82676607-82676626 MsG0380016378.01.T01:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG - Chr3:82676420-82676439 None:intergenic 35.0%
! GTATTTGCTGGAAAAGAAGA+AGG + Chr3:82676928-82676947 MsG0380016378.01.T01:CDS 35.0%
! GTGATAAAGAAGACCCTTTT+TGG - Chr3:82675846-82675865 None:intergenic 35.0%
! TTGGAGGGATGATATTTTGT+TGG - Chr3:82676479-82676498 None:intergenic 35.0%
!! AAGCACTTTTTACACACCTA+TGG + Chr3:82676497-82676516 MsG0380016378.01.T01:CDS 35.0%
!! ATAGGTGTGTAAAAAGTGCT+TGG - Chr3:82676498-82676517 None:intergenic 35.0%
!! TAAAGAAGACCCTTTTTGGT+GGG - Chr3:82675842-82675861 None:intergenic 35.0%
!! TGAATTTGATTTGGCACTAG+AGG - Chr3:82675872-82675891 None:intergenic 35.0%
!! TGGTTTTACATTTAACCACG+CGG + Chr3:82676189-82676208 MsG0380016378.01.T01:CDS 35.0%
!! TGTGATCTGTTATTTCGAGA+CGG - Chr3:82675949-82675968 None:intergenic 35.0%
!! TTTTGTGGCTCTATAGTGAA+AGG - Chr3:82676349-82676368 None:intergenic 35.0%
AAAAATGAATCGACGATGGG+AGG - Chr3:82676082-82676101 None:intergenic 40.0%
AATCACTACGAGTCTTGGTT+GGG + Chr3:82676754-82676773 MsG0380016378.01.T01:CDS 40.0%
AATGAAAGCAGGACAGTTGT+TGG + Chr3:82676657-82676676 MsG0380016378.01.T01:CDS 40.0%
ACACAACATCCATGAAGTCA+TGG - Chr3:82677011-82677030 None:intergenic 40.0%
ACCATAAACATCAAACCACG+GGG - Chr3:82676842-82676861 None:intergenic 40.0%
ACTAATTGCACCCACCAAAA+AGG + Chr3:82675829-82675848 MsG0380016378.01.T01:CDS 40.0%
ACTTGATTGTATGGGTAGAG+AGG - Chr3:82675754-82675773 None:intergenic 40.0%
ATCGGAGTTCAAAACTACGA+AGG + Chr3:82676109-82676128 MsG0380016378.01.T01:CDS 40.0%
ATGGGATGTTGAATACCACT+TGG - Chr3:82676319-82676338 None:intergenic 40.0%
ATTGCCTCCAAAATCTTGTG+AGG - Chr3:82676979-82676998 None:intergenic 40.0%
ATTTAACTCCATGGGATCTC+CGG + Chr3:82675801-82675820 MsG0380016378.01.T01:CDS 40.0%
CAAGTGACTGAGTTAGTTGA+CGG + Chr3:82676157-82676176 MsG0380016378.01.T01:CDS 40.0%
CTAATTGCACCCACCAAAAA+GGG + Chr3:82675830-82675849 MsG0380016378.01.T01:CDS 40.0%
GTGGGTGAAATTGTTTGGAA+CGG - Chr3:82676534-82676553 None:intergenic 40.0%
TGAAGAATCACTACGAGTCT+TGG + Chr3:82676749-82676768 MsG0380016378.01.T01:CDS 40.0%
TGTAGGGTTACAATGAAAGC+AGG + Chr3:82676646-82676665 MsG0380016378.01.T01:CDS 40.0%
TTAGTTGACGGCATCTTCAT+TGG + Chr3:82676169-82676188 MsG0380016378.01.T01:CDS 40.0%
TTCGTGTGGGTGAAATTGTT+TGG - Chr3:82676539-82676558 None:intergenic 40.0%
TTGAAAGCCAACATGGAGAT+TGG + Chr3:82676451-82676470 MsG0380016378.01.T01:CDS 40.0%
! AACCTTGATCTAGCTCCTAT+CGG - Chr3:82676580-82676599 None:intergenic 40.0%
! GTGTCTTCCTCACAAGATTT+TGG + Chr3:82676969-82676988 MsG0380016378.01.T01:CDS 40.0%
! TCATCAGAGTCGTTCTTTTG+TGG - Chr3:82676364-82676383 None:intergenic 40.0%
! TCTTCCTCACAAGATTTTGG+AGG + Chr3:82676972-82676991 MsG0380016378.01.T01:CDS 40.0%
!! CACAAGATTTTGGAGGCAAT+GGG + Chr3:82676979-82676998 MsG0380016378.01.T01:CDS 40.0%
!! GTGTGTAAAAAGTGCTTGGA+GGG - Chr3:82676494-82676513 None:intergenic 40.0%
!! TCACAAGATTTTGGAGGCAA+TGG + Chr3:82676978-82676997 MsG0380016378.01.T01:CDS 40.0%
!! TTTGATTTGGCACTAGAGGA+TGG - Chr3:82675868-82675887 None:intergenic 40.0%
!! TTTTGGTGGGTGCAATTAGT+AGG - Chr3:82675829-82675848 None:intergenic 40.0%
AAACCACGGGGAATTGCTAT+TGG - Chr3:82676830-82676849 None:intergenic 45.0%
ACCAGCATTGTTGCATGTGA+CGG - Chr3:82675993-82676012 None:intergenic 45.0%
ACTTGCGTAGGTGACATACT+TGG + Chr3:82676043-82676062 MsG0380016378.01.T01:CDS 45.0%
GAATCACTACGAGTCTTGGT+TGG + Chr3:82676753-82676772 MsG0380016378.01.T01:CDS 45.0%
GACAGTTGTTGGAAGATGGT+GGG + Chr3:82676668-82676687 MsG0380016378.01.T01:CDS 45.0%
GGGAAATGACCATGACTTCA+TGG + Chr3:82676999-82677018 MsG0380016378.01.T01:CDS 45.0%
GTCACTTGCACAGCTAACAA+TGG - Chr3:82676145-82676164 None:intergenic 45.0%
GTTGGTACCAATCTCCATGT+TGG - Chr3:82676461-82676480 None:intergenic 45.0%
TCCCCGTGGTTTGATGTTTA+TGG + Chr3:82676838-82676857 MsG0380016378.01.T01:CDS 45.0%
TGGGTGCAATTAGTAGGTAC+CGG - Chr3:82675823-82675842 None:intergenic 45.0%
TTGTATGGGTAGAGAGGACT+TGG - Chr3:82675748-82675767 None:intergenic 45.0%
TTTAACCACGCGGTTGTTGA+TGG + Chr3:82676199-82676218 MsG0380016378.01.T01:CDS 45.0%
! AAGTGGTGGGAAAAAGGCAA+GGG - Chr3:82675921-82675940 None:intergenic 45.0%
! ACCTTGATCTAGCTCCTATC+GGG - Chr3:82676579-82676598 None:intergenic 45.0%
! GCATGTGACGGAACATGAAA+TGG - Chr3:82675981-82676000 None:intergenic 45.0%
! TTGTTGATGGAGAGTCCACA+TGG + Chr3:82676212-82676231 MsG0380016378.01.T01:CDS 45.0%
! TTTTGGATGGGGAAAACCTG+TGG + Chr3:82676867-82676886 MsG0380016378.01.T01:CDS 45.0%
!! CTAGAACGTTGGTTTCCAAG+TGG + Chr3:82676301-82676320 MsG0380016378.01.T01:CDS 45.0%
!! GGTGTGTAAAAAGTGCTTGG+AGG - Chr3:82676495-82676514 None:intergenic 45.0%
AAACCTGTGGCAATTCGCAG+TGG + Chr3:82676880-82676899 MsG0380016378.01.T01:CDS 50.0%
GCAGGACAGTTGTTGGAAGA+TGG + Chr3:82676664-82676683 MsG0380016378.01.T01:CDS 50.0%
GGACAGTTGTTGGAAGATGG+TGG + Chr3:82676667-82676686 MsG0380016378.01.T01:CDS 50.0%
TAGCCAATAGCAATTCCCCG+TGG + Chr3:82676824-82676843 MsG0380016378.01.T01:CDS 50.0%
TCCCGATAGGAGCTAGATCA+AGG + Chr3:82676575-82676594 MsG0380016378.01.T01:CDS 50.0%
TCCGTCACATGCAACAATGC+TGG + Chr3:82675989-82676008 MsG0380016378.01.T01:CDS 50.0%
TTTCCACTGCGAATTGCCAC+AGG - Chr3:82676886-82676905 None:intergenic 50.0%
! CAAGTGGTGGGAAAAAGGCA+AGG - Chr3:82675922-82675941 None:intergenic 50.0%
! TCATGGGCTAAAATCTCGCG+TGG + Chr3:82676247-82676266 MsG0380016378.01.T01:CDS 50.0%
!! GAAGATGGTGGGCTTGGTAA+AGG + Chr3:82676679-82676698 MsG0380016378.01.T01:CDS 50.0%
!! TGGGCTTGGTAAAGGTGCTT+GGG + Chr3:82676687-82676706 MsG0380016378.01.T01:CDS 50.0%
!! TTGTTGGAAGATGGTGGGCT+TGG + Chr3:82676673-82676692 MsG0380016378.01.T01:CDS 50.0%
ACTCTCCATCAACAACCGCG+TGG - Chr3:82676207-82676226 None:intergenic 55.0%
ATCGACGATGGGAGGAACGT+AGG - Chr3:82676074-82676093 None:intergenic 55.0%
ATTTCGAGACGGCCTGCAAG+TGG - Chr3:82675938-82675957 None:intergenic 55.0%
TACGCAAGTGTTCTCCGCTG+CGG - Chr3:82676032-82676051 None:intergenic 55.0%
! AACGGATCACAGAGCGCCAT+AGG - Chr3:82676516-82676535 None:intergenic 55.0%
! GCCTGCAAGTGGTGGGAAAA+AGG - Chr3:82675927-82675946 None:intergenic 55.0%
! GCCTTTTTCCCACCACTTGC+AGG + Chr3:82675923-82675942 MsG0380016378.01.T01:CDS 55.0%
!! AGTAGGTACCGGAGATCCCA+TGG - Chr3:82675812-82675831 None:intergenic 55.0%
!! GTGGGCTTGGTAAAGGTGCT+TGG + Chr3:82676686-82676705 MsG0380016378.01.T01:CDS 55.0%
AAGTGTTCTCCGCTGCGGCA+TGG - Chr3:82676027-82676046 None:intergenic 60.0%
GCAGCGGAGAACACTTGCGT+AGG + Chr3:82676031-82676050 MsG0380016378.01.T01:CDS 60.0%
GCTCTTTGTCCATGCCGCAG+CGG + Chr3:82676015-82676034 MsG0380016378.01.T01:CDS 60.0%
CGAGACGGCCTGCAAGTGGT+GGG - Chr3:82675934-82675953 None:intergenic 65.0%
TCGAGACGGCCTGCAAGTGG+TGG - Chr3:82675935-82675954 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 82675734 82677038 82675734 ID=MsG0380016378.01;Name=MsG0380016378.01
Chr3 mRNA 82675734 82677038 82675734 ID=MsG0380016378.01.T01;Parent=MsG0380016378.01;Name=MsG0380016378.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|434
Chr3 exon 82675734 82677038 82675734 ID=MsG0380016378.01.T01:exon:33137;Parent=MsG0380016378.01.T01
Chr3 CDS 82675734 82677038 82675734 ID=MsG0380016378.01.T01:cds;Parent=MsG0380016378.01.T01
Gene Sequence

>MsG0380016378.01.T01

ATGGCTGCAATCCAAGTCCTCTCTACCCATACAATCAAGTCACCAAATTCAATTGATCAAAAAATTGATTTAACTCCATGGGATCTCCGGTACCTACTAATTGCACCCACCAAAAAGGGTCTTCTTTATCACCATCCTCTAGTGCCAAATCAAATTCAACATCTGAAACACTCTCTATCTTCTGCCCTTGCCTTTTTCCCACCACTTGCAGGCCGTCTCGAAATAACAGATCACAAAGACAACACCATTTCATGTTCCGTCACATGCAACAATGCTGGTGCGCTCTTTGTCCATGCCGCAGCGGAGAACACTTGCGTAGGTGACATACTTGGATGCACCTACGTTCCTCCCATCGTCGATTCATTTTTTCCATTAATCGGAGTTCAAAACTACGAAGGCACGTCACAACCATTGTTAGCTGTGCAAGTGACTGAGTTAGTTGACGGCATCTTCATTGGTTTTACATTTAACCACGCGGTTGTTGATGGAGAGTCCACATGGCTTTTCATTAACTCATGGGCTAAAATCTCGCGTGGTTGTTGCAATCAAGTATCCAAACTCATAACACTAGAACGTTGGTTTCCAAGTGGTATTCAACATCCCATACGATTTCCTTTCACTATAGAGCCACAAAAGAACGACTCTGATGATAACGAAAAATTCAATCCATCAGAGAGGTTATTCCATTTCACAAAGGAGAAAATCGCTCAACTGAAATTGAAAGCCAACATGGAGATTGGTACCAACAAAATATCATCCCTCCAAGCACTTTTTACACACCTATGGCGCTCTGTGATCCGTTCCAAACAATTTCACCCACACGAAGAAGTTTATTATATGGTCCCGATAGGAGCTAGATCAAGGTTTGTTCCACCATTGCCTGAAGATTATTTTGGAAATGCTGTTGAAGTTTGTAGGGTTACAATGAAAGCAGGACAGTTGTTGGAAGATGGTGGGCTTGGTAAAGGTGCTTGGGCGATACACAAAATGATTTCTTTGCAGTCAAATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTGGGAAATCCAAACATTGTTAGATTTTTTAGTGCGACCAATAATAATACATTAGCCAATAGCAATTCCCCGTGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAACCTGTGGCAATTCGCAGTGGAAATAAAATAAGTGGAATGATTACTGTATTTGCTGGAAAAGAAGAAGGTAGTATGGACTTTCAAGTGTGTCTTCCTCACAAGATTTTGGAGGCAATGGGAAATGACCATGACTTCATGGATGTTGTGTCTATTTAA
Protein sequence

>MsG0380016378.01.T01

MAAIQVLSTHTIKSPNSIDQKIDLTPWDLRYLLIAPTKKGLLYHHPLVPNQIQHLKHSLSSALAFFPPLAGRLEITDHKDNTISCSVTCNNAGALFVHAAAENTCVGDILGCTYVPPIVDSFFPLIGVQNYEGTSQPLLAVQVTELVDGIFIGFTFNHAVVDGESTWLFINSWAKISRGCCNQVSKLITLERWFPSGIQHPIRFPFTIEPQKNDSDDNEKFNPSERLFHFTKEKIAQLKLKANMEIGTNKISSLQALFTHLWRSVIRSKQFHPHEEVYYMVPIGARSRFVPPLPEDYFGNAVEVCRVTMKAGQLLEDGGLGKGAWAIHKMISLQSNEKLKNHYESWLGNPNIVRFFSATNNNTLANSNSPWFDVYGNDFGWGKPVAIRSGNKISGMITVFAGKEEGSMDFQVCLPHKILEAMGNDHDFMDVVSI*