AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045884.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045884.01.T01 MTR_4g094835 72.026 454 117 3 1 452 1 446 0.0 677
MsG0880045884.01.T01 MTR_4g094838 71.930 456 99 7 4 453 1 433 0.0 633
MsG0880045884.01.T01 MTR_4g094832 73.475 377 91 3 81 453 60 431 0.0 560
MsG0880045884.01.T01 MTR_4g094842 60.352 454 164 8 1 448 1 444 0.0 541
MsG0880045884.01.T01 MTR_0190s0020 59.202 451 168 8 1 448 1 438 0.0 527
MsG0880045884.01.T01 MTR_8g041770 57.650 451 174 8 1 448 1 437 0.0 515
MsG0880045884.01.T01 MTR_3g088420 58.000 450 166 10 1 448 1 429 7.12e-178 504
MsG0880045884.01.T01 MTR_4g094828 56.608 454 175 10 1 444 1 442 1.46e-176 502
MsG0880045884.01.T01 MTR_4g022720 56.512 453 170 9 1 448 1 431 2.02e-176 501
MsG0880045884.01.T01 MTR_4g022825 56.140 456 172 10 1 448 1 436 1.38e-174 496
MsG0880045884.01.T01 MTR_8g079710 57.965 452 165 11 1 443 1 436 3.14e-174 496
MsG0880045884.01.T01 MTR_4g022800 55.895 458 172 10 1 448 1 438 1.08e-173 494
MsG0880045884.01.T01 MTR_4g022290 57.489 454 166 13 1 444 1 437 3.91e-172 490
MsG0880045884.01.T01 MTR_4g022670 54.705 457 178 10 1 448 1 437 1.28e-169 484
MsG0880045884.01.T01 MTR_3g026310 54.212 463 178 11 1 448 1 444 6.24e-168 480
MsG0880045884.01.T01 MTR_7g026860 54.605 456 177 11 1 448 1 434 5.60e-164 469
MsG0880045884.01.T01 MTR_4g022760 49.342 456 160 10 1 448 1 393 5.20e-142 412
MsG0880045884.01.T01 MTR_6g016815 42.889 450 168 13 1 448 15 377 5.93e-105 317
MsG0880045884.01.T01 MTR_3g101900 40.000 430 238 9 28 448 26 444 7.27e-95 293
MsG0880045884.01.T01 MTR_4g094825 85.632 174 22 2 20 193 80 250 9.83e-95 291
MsG0880045884.01.T01 MTR_4g094825 85.161 155 13 1 299 453 248 392 6.69e-86 268
MsG0880045884.01.T01 MTR_3g102020 38.445 463 248 11 3 448 5 447 7.33e-94 291
MsG0880045884.01.T01 MTR_3g101930 39.359 437 230 10 32 448 30 451 2.48e-92 287
MsG0880045884.01.T01 MTR_3g102010 39.443 431 234 9 32 448 30 447 3.26e-89 279
MsG0880045884.01.T01 MTR_3g101960 37.527 461 252 11 3 447 5 445 1.47e-88 277
MsG0880045884.01.T01 MTR_3g101970 37.850 428 232 9 32 448 29 433 3.70e-86 271
MsG0880045884.01.T01 MTR_5g081960 33.333 483 243 9 30 453 25 487 5.41e-80 256
MsG0880045884.01.T01 MTR_3g070170 35.358 461 239 10 30 452 31 470 1.25e-78 252
MsG0880045884.01.T01 MTR_8g040780 58.937 207 80 3 241 444 20 224 1.19e-77 242
MsG0880045884.01.T01 MTR_8g040760 54.867 226 88 5 1 223 1 215 8.54e-76 236
MsG0880045884.01.T01 MTR_4g015180 32.716 486 244 11 27 448 17 483 1.19e-70 232
MsG0880045884.01.T01 MTR_7g026850 54.598 174 70 3 37 208 12 178 5.80e-58 189
MsG0880045884.01.T01 MTR_3g102030 32.523 329 182 5 82 406 28 320 4.66e-50 173
MsG0880045884.01.T01 MTR_6g045553 27.950 483 293 12 2 449 10 472 2.07e-48 172
MsG0880045884.01.T01 MTR_7g026770 49.171 181 65 4 156 334 132 287 6.78e-46 161
MsG0880045884.01.T01 MTR_6g034485 56.154 130 52 2 201 330 1 125 2.73e-44 151
MsG0880045884.01.T01 MTR_6g034495 59.055 127 44 2 72 193 37 160 9.36e-44 151
MsG0880045884.01.T01 MTR_7g026830 68.293 82 26 0 99 180 16 97 3.10e-33 121
MsG0880045884.01.T01 MTR_4g007540 26.343 391 255 10 64 441 56 426 2.88e-29 119
MsG0880045884.01.T01 MTR_3g098980 27.877 391 239 17 57 434 59 419 2.16e-28 117
MsG0880045884.01.T01 MTR_8g096530 30.110 362 211 13 57 404 52 385 2.31e-28 117
MsG0880045884.01.T01 MTR_3g049150 25.688 436 272 13 37 439 21 437 1.09e-26 112
MsG0880045884.01.T01 MTR_2g105330 24.181 397 258 10 64 437 56 432 3.96e-26 110
MsG0880045884.01.T01 MTR_3g026950 24.755 408 255 15 61 437 51 437 3.24e-25 107
MsG0880045884.01.T01 MTR_2g064495 23.822 382 259 9 64 433 56 417 3.82e-25 107
MsG0880045884.01.T01 MTR_8g075610 25.000 396 250 15 64 441 47 413 6.84e-25 106
MsG0880045884.01.T01 MTR_4g097090 25.057 439 258 16 40 433 28 440 1.82e-24 105
MsG0880045884.01.T01 MTR_4g127650 22.748 444 287 14 22 433 21 440 2.65e-22 99.4
MsG0880045884.01.T01 MTR_7g026780 47.222 108 31 3 341 448 26 107 8.71e-22 90.5
MsG0880045884.01.T01 MTR_3g071320 25.970 335 205 11 49 361 42 355 8.89e-22 97.8
MsG0880045884.01.T01 MTR_7g027175 26.276 392 257 12 57 439 61 429 1.42e-21 97.1
MsG0880045884.01.T01 MTR_1g081660 23.702 443 294 16 32 452 31 451 1.28e-20 94.4
MsG0880045884.01.T01 MTR_6g015850 24.359 468 302 15 2 441 6 449 1.36e-20 94.4
MsG0880045884.01.T01 MTR_7g026800 39.216 153 61 6 239 389 38 160 1.72e-19 86.7
MsG0880045884.01.T01 MTR_6g015815 23.182 440 291 14 30 441 32 452 3.02e-19 90.1
MsG0880045884.01.T01 MTR_5g026320 24.819 415 247 15 61 447 71 448 6.40e-19 89.4
MsG0880045884.01.T01 MTR_6g015315 26.250 320 199 9 154 441 141 455 6.97e-19 89.0
MsG0880045884.01.T01 MTR_4g022780 37.821 156 65 6 239 392 39 164 6.98e-19 84.3
MsG0880045884.01.T01 MTR_2g089765 25.519 482 282 22 3 436 15 467 1.58e-18 88.2
MsG0880045884.01.T01 MTR_7g084940 25.967 362 230 12 52 401 57 392 1.74e-18 87.8
MsG0880045884.01.T01 MTR_5g084870 25.693 397 231 17 33 402 17 376 2.83e-18 87.0
MsG0880045884.01.T01 MTR_3g086020 25.845 414 229 18 62 453 59 416 7.49e-18 85.9
MsG0880045884.01.T01 MTR_7g014330 24.691 405 253 15 30 401 24 409 7.62e-18 85.9
MsG0880045884.01.T01 MTR_3g049140 27.230 213 134 5 243 439 27 234 8.63e-18 83.2
MsG0880045884.01.T01 MTR_6g015320 26.582 316 187 12 154 439 144 444 9.27e-18 85.9
MsG0880045884.01.T01 MTR_1g040195 23.982 442 256 18 33 446 23 412 1.95e-17 84.7
MsG0880045884.01.T01 MTR_8g012750 25.060 415 252 16 30 401 24 422 4.09e-17 84.0
MsG0880045884.01.T01 MTR_2g089755 26.471 408 249 19 32 402 37 430 8.51e-17 82.8
MsG0880045884.01.T01 MTR_8g079980 24.060 399 240 11 33 402 17 381 2.20e-16 81.3
MsG0880045884.01.T01 MTR_7g114470 23.690 439 268 18 33 436 25 431 4.15e-16 80.9
MsG0880045884.01.T01 MTR_7g014250 25.301 415 238 18 30 401 24 409 4.16e-16 80.9
MsG0880045884.01.T01 MTR_7g085930 23.601 411 250 14 64 428 59 451 6.34e-16 80.1
MsG0880045884.01.T01 MTR_5g076700 22.297 444 287 16 33 446 24 439 9.34e-16 79.7
MsG0880045884.01.T01 MTR_7g086000 25.369 406 263 13 62 441 58 449 1.06e-15 79.3
MsG0880045884.01.T01 MTR_2g089670 23.981 417 244 16 29 401 42 429 2.09e-15 78.6
MsG0880045884.01.T01 MTR_7g014200 24.654 434 238 18 23 401 21 420 1.26e-14 76.3
MsG0880045884.01.T01 MTR_7g014230 24.257 404 256 15 30 401 26 411 1.41e-14 75.9
MsG0880045884.01.T01 MTR_4g055170 24.752 303 206 7 64 360 69 355 2.06e-14 75.5
MsG0880045884.01.T01 MTR_4g013050 21.463 410 248 12 57 443 46 404 2.17e-14 75.1
MsG0880045884.01.T01 MTR_3g450710 25.707 389 216 16 58 406 59 414 2.57e-14 75.1
MsG0880045884.01.T01 MTR_2g089650 23.961 409 272 17 23 402 20 418 2.70e-14 75.1
MsG0880045884.01.T01 MTR_7g014160 23.207 474 285 19 17 433 4 455 3.61e-14 74.7
MsG0880045884.01.T01 MTR_7g114490 23.788 433 259 19 37 439 40 431 1.13e-13 73.2
MsG0880045884.01.T01 MTR_7g114520 23.919 393 248 19 37 409 41 402 1.44e-13 72.8
MsG0880045884.01.T01 MTR_5g090830 25.401 374 223 15 61 402 63 412 1.90e-13 72.4
MsG0880045884.01.T01 MTR_1g053315 21.762 386 268 15 64 433 78 445 2.06e-13 72.4
MsG0880045884.01.T01 MTR_3g112180 22.636 349 243 11 61 400 58 388 3.59e-13 71.6
MsG0880045884.01.T01 MTR_8g467580 26.218 431 248 20 49 448 41 432 3.69e-13 71.6
MsG0880045884.01.T01 MTR_7g056087 23.227 409 265 16 40 418 30 419 4.31e-13 71.2
MsG0880045884.01.T01 MTR_5g090880 24.474 380 228 16 57 402 60 414 6.09e-13 70.9
MsG0880045884.01.T01 MTR_1g040315 24.503 302 193 10 33 319 23 304 1.31e-12 69.7
MsG0880045884.01.T01 MTR_7g085990 22.837 416 247 15 64 428 55 447 1.48e-12 69.7
MsG0880045884.01.T01 MTR_1g094790 24.222 450 256 21 40 448 32 437 6.70e-12 67.8
MsG0880045884.01.T01 MTR_7g084910 24.161 298 188 9 42 318 38 318 7.03e-12 67.4
MsG0880045884.01.T01 MTR_7g026870 45.714 70 32 2 297 360 82 151 7.91e-12 63.5
MsG0880045884.01.T01 MTR_3g028210 24.411 467 290 23 4 433 8 448 8.26e-12 67.4
MsG0880045884.01.T01 MTR_7g026880 57.778 45 19 0 404 448 5 49 1.01e-11 60.1
MsG0880045884.01.T01 MTR_8g467590 25.000 412 245 15 63 448 59 432 2.08e-11 66.2
MsG0880045884.01.T01 MTR_2g015430 24.716 352 227 13 64 402 58 384 3.20e-11 65.5
MsG0880045884.01.T01 MTR_7g013850 22.169 415 254 14 37 401 37 432 8.52e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045884.01.T01 AT5G67150 41.538 455 243 7 2 448 3 442 1.63e-117 352
MsG0880045884.01.T01 AT3G50300 40.481 457 249 8 1 448 1 443 4.13e-104 317
MsG0880045884.01.T01 AT3G50280 37.389 452 266 6 1 448 1 439 3.35e-100 307
MsG0880045884.01.T01 AT3G50270 39.955 448 254 8 6 448 7 444 6.46e-99 304
MsG0880045884.01.T01 AT5G38130 39.401 434 247 6 5 433 20 442 4.50e-98 302
MsG0880045884.01.T01 AT5G67160 39.252 428 233 8 25 447 22 427 1.25e-96 297
MsG0880045884.01.T01 AT5G42830 36.028 433 252 9 28 448 26 445 7.37e-86 270
MsG0880045884.01.T01 AT2G39980 33.546 471 240 9 30 448 27 476 3.04e-81 259
MsG0880045884.01.T01 AT5G07850 36.574 432 246 10 32 448 31 449 1.47e-79 254
MsG0880045884.01.T01 AT5G07860 36.866 434 238 11 32 448 31 445 2.95e-79 253
MsG0880045884.01.T01 AT5G01210 33.406 458 249 8 30 448 25 465 2.28e-74 241
MsG0880045884.01.T01 AT5G07870 35.294 442 246 9 32 448 31 457 1.98e-73 238
MsG0880045884.01.T01 AT3G50280 39.103 312 184 3 140 448 1 309 4.21e-72 230
MsG0880045884.01.T01 AT5G23940 31.900 442 268 10 28 449 21 449 3.17e-61 207
MsG0880045884.01.T01 AT3G50290 39.925 268 154 3 137 399 4 269 4.54e-58 192
MsG0880045884.01.T01 AT2G19070 25.909 440 278 12 35 441 19 443 8.84e-29 118
MsG0880045884.01.T01 AT3G48720 27.119 354 228 12 57 402 54 385 7.49e-27 112
MsG0880045884.01.T01 AT3G29590 25.734 443 286 14 24 443 20 442 3.88e-26 110
MsG0880045884.01.T01 AT5G48930 25.000 408 261 11 49 436 39 421 9.83e-26 109
MsG0880045884.01.T01 AT5G41040 26.667 390 248 11 57 433 62 426 1.11e-25 109
MsG0880045884.01.T01 AT5G41040 26.667 390 248 11 57 433 78 442 1.34e-25 109
MsG0880045884.01.T01 AT5G57840 27.338 417 238 17 49 433 39 422 4.39e-22 98.6
MsG0880045884.01.T01 AT3G29635 24.312 436 274 15 30 433 27 438 4.84e-22 98.6
MsG0880045884.01.T01 AT1G78990 27.763 371 223 13 51 402 52 396 1.22e-21 97.4
MsG0880045884.01.T01 AT5G63560 24.615 390 250 14 57 433 54 412 2.06e-20 93.6
MsG0880045884.01.T01 AT1G24430 23.499 383 243 14 55 425 53 397 3.40e-19 90.1
MsG0880045884.01.T01 AT1G31490 26.098 410 256 15 61 453 59 438 5.42e-19 89.4
MsG0880045884.01.T01 AT5G23970 24.598 435 279 17 28 449 20 418 4.45e-18 86.7
MsG0880045884.01.T01 AT5G39050 25.240 313 199 12 150 434 145 450 5.88e-18 86.7
MsG0880045884.01.T01 AT2G40230 25.121 414 271 13 1 402 1 387 8.60e-18 85.9
MsG0880045884.01.T01 AT1G28680 27.451 255 167 9 156 402 143 387 1.13e-17 85.5
MsG0880045884.01.T01 AT5G47950 23.362 458 298 16 1 448 1 415 2.14e-17 84.3
MsG0880045884.01.T01 AT3G29670 23.799 458 296 17 3 433 2 433 2.43e-17 84.3
MsG0880045884.01.T01 AT3G30280 25.641 429 249 21 1 402 1 386 2.80e-17 84.3
MsG0880045884.01.T01 AT1G24420 25.255 392 243 16 74 452 75 429 1.05e-16 82.4
MsG0880045884.01.T01 AT1G24420 25.255 392 243 16 74 452 75 429 1.05e-16 82.4
MsG0880045884.01.T01 AT5G39090 25.682 440 281 20 20 436 16 432 1.19e-16 82.4
MsG0880045884.01.T01 AT3G26040 24.384 406 255 14 60 448 55 425 1.45e-16 82.0
MsG0880045884.01.T01 AT1G28680 28.095 210 138 7 156 358 143 346 2.19e-16 81.3
MsG0880045884.01.T01 AT5G61160 25.890 309 201 13 150 439 139 438 3.31e-15 77.8
MsG0880045884.01.T01 AT5G07080 24.595 309 194 10 42 332 44 331 3.02e-14 75.1
MsG0880045884.01.T01 AT5G16410 25.816 337 193 16 94 402 112 419 2.67e-13 72.0
MsG0880045884.01.T01 AT3G47170 23.279 305 198 10 57 340 63 352 8.29e-13 70.5
MsG0880045884.01.T01 AT5G47980 25.182 413 242 20 63 448 60 432 7.61e-12 67.4
MsG0880045884.01.T01 AT2G25150 24.427 262 182 8 154 405 148 403 9.71e-12 67.4
MsG0880045884.01.T01 AT1G65450 22.161 361 244 13 55 401 63 400 2.48e-11 65.9

Find 93 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATTCTTTCAGCTAAAT+TGG 0.161367 8:+65970130 None:intergenic
GTGTCTTTCTTATGAGATTT+TGG 0.228049 8:-65969547 MsG0880045884.01.T01:CDS
CGTTGAAATGAAGGGAGTTT+TGG 0.257934 8:+65970245 None:intergenic
TCATTCTTCCCACCCTTTGC+AGG 0.261438 8:-65970623 MsG0880045884.01.T01:CDS
TATGGGAACCTTTCGAGATT+TGG 0.266155 8:+65970273 None:intergenic
GGGAAATGATGTTGAGTTCT+TGG 0.297726 8:-65969517 MsG0880045884.01.T01:CDS
TTTGGCATTTCGTTAATTCT+TGG 0.304274 8:-65970298 MsG0880045884.01.T01:CDS
TACATTCTGATGAGAAAATA+AGG 0.315586 8:-65969791 MsG0880045884.01.T01:CDS
GGAAACGCAGTAGTGGTTAA+TGG 0.325663 8:-65969891 MsG0880045884.01.T01:CDS
ATGGGAACCTTTCGAGATTT+GGG 0.326542 8:+65970274 None:intergenic
GGTGGAAAAGAAGGCCTCTT+TGG 0.329077 8:+65970705 None:intergenic
TGAGATGGTTGATTTGGTTT+TGG 0.333677 8:+65970669 None:intergenic
AAGCACTATTATCTCATGTT+TGG 0.349361 8:-65970022 MsG0880045884.01.T01:CDS
TAAGGAACCATTACGAATCT+TGG 0.357996 8:-65969773 MsG0880045884.01.T01:CDS
TTTGTTTGAGATGGTTGATT+TGG 0.369144 8:+65970663 None:intergenic
ACCAAGGATGATTCCACCTT+TGG 0.373650 8:-65969928 MsG0880045884.01.T01:CDS
ATTGGAATTTCAATATCGTT+TGG 0.377241 8:+65970215 None:intergenic
TTGGAATTTCAATATCGTTT+GGG 0.377876 8:+65970216 None:intergenic
TTGTAGACGCCAAGTCGTTT+TGG 0.392431 8:-65970316 MsG0880045884.01.T01:CDS
TGTTGTGGATATCACCTTGA+TGG 0.409513 8:+65970832 None:intergenic
AAAACCAACTCTAGCAATTC+AGG 0.414621 8:-65970390 MsG0880045884.01.T01:CDS
TTAAGTGACGGCATCTTCAT+TGG 0.417429 8:-65970359 MsG0880045884.01.T01:CDS
CCTTTGGAAGAAGATTATTT+CGG 0.422258 8:-65969912 MsG0880045884.01.T01:CDS
TGTGTGAAAGGGAAGCGTAT+TGG 0.429614 8:+65970197 None:intergenic
CAGTCACATTACTTAGAACT+AGG 0.433385 8:+65969492 None:intergenic
GTTTGTGGTGTCGTTGTGTT+GGG 0.434146 8:+65970586 None:intergenic
CATTCTTCCCACCCTTTGCA+GGG 0.439523 8:-65970622 MsG0880045884.01.T01:CDS
AGCTTTCTTCAAAGGAGTCT+TGG 0.442985 8:+65970159 None:intergenic
TCCAAAGGTGGAATCATCCT+TGG 0.454320 8:+65969927 None:intergenic
TGTTTGTGGTGTCGTTGTGT+TGG 0.462700 8:+65970585 None:intergenic
TTTGGTTAGGAATGGGTTGG+TGG 0.465905 8:+65970687 None:intergenic
CCGAAATAATCTTCTTCCAA+AGG 0.467292 8:+65969912 None:intergenic
AGATTATTATTGTTGTCAAT+TGG 0.472545 8:+65970797 None:intergenic
GGGAACCAACGTTGAAATGA+AGG 0.474068 8:+65970236 None:intergenic
GACAATTGGAGGTGTGTAGT+TGG 0.479530 8:+65970460 None:intergenic
TGGAAAGTGTTTGTTTGAGA+TGG 0.485281 8:+65970654 None:intergenic
TTCTCAACCAAGATTCGTAA+TGG 0.489014 8:+65969766 None:intergenic
GAGGTTCTAGAGTGTGAAAT+CGG 0.491146 8:-65969849 MsG0880045884.01.T01:CDS
AACGAAATGCCAAAACGACT+TGG 0.492743 8:+65970307 None:intergenic
TGTGATTCTATTTGTGTGAA+AGG 0.494618 8:+65970185 None:intergenic
GAATGGGTTGGTGGAAAAGA+AGG 0.503869 8:+65970696 None:intergenic
GGATTCTAAGCTTTCTTCAA+AGG 0.505903 8:+65970151 None:intergenic
CTTCTTCATGGATTCCATCA+AGG 0.511613 8:-65970846 None:intergenic
AATGGTGTGAGGATGAAAGC+AGG 0.512088 8:-65969873 MsG0880045884.01.T01:CDS
GTTACCTGAATTGCTAGAGT+TGG 0.515092 8:+65970386 None:intergenic
TGGTTTCAAGTGGAAGGAAT+CGG 0.519621 8:+65970729 None:intergenic
GAGGTGTGTAGTTGGATTGA+AGG 0.522441 8:+65970468 None:intergenic
TTTGGATGGTTTCAAGTGGA+AGG 0.524015 8:+65970723 None:intergenic
GAAAAGAAGGCCTCTTTGGA+TGG 0.529505 8:+65970709 None:intergenic
GTGATTCTATTTGTGTGAAA+GGG 0.534325 8:+65970186 None:intergenic
CTCCCACTTCGAAACGCCAC+AGG 0.534418 8:+65969639 None:intergenic
TTCCGAAGTGGTAGTTCGCT+AGG 0.540203 8:-65969732 MsG0880045884.01.T01:CDS
ACCGAGGTTGTTGCAAATGA+TGG 0.541381 8:+65970538 None:intergenic
GATTACAAGACGCCCTGCAA+AGG 0.543778 8:+65970610 None:intergenic
AGCCTGTGGCGTTTCGAAGT+GGG 0.545492 8:-65969641 MsG0880045884.01.T01:CDS
AACTCCCTTCATTTCAACGT+TGG 0.545872 8:-65970241 MsG0880045884.01.T01:CDS
GTGGCGTTTCGAAGTGGGAG+TGG 0.549176 8:-65969636 MsG0880045884.01.T01:CDS
ATGGTGTGAGGATGAAAGCA+GGG 0.551554 8:-65969872 MsG0880045884.01.T01:CDS
GTGTTTGCTGGTGCTGAAGA+AGG 0.553326 8:-65969588 MsG0880045884.01.T01:CDS
ATTACAAGACGCCCTGCAAA+GGG 0.555357 8:+65970611 None:intergenic
TTCGCTAGGTGGTAATATGT+TGG 0.564328 8:-65969718 MsG0880045884.01.T01:CDS
AAATAATCTTCTTCCAAAGG+TGG 0.566011 8:+65969915 None:intergenic
TGGTGCTTGAATTGTTGTTG+TGG 0.571268 8:+65970817 None:intergenic
GGAACCAACGTTGAAATGAA+GGG 0.576282 8:+65970237 None:intergenic
TCTTGGTCCCAAATCTCGAA+AGG 0.578370 8:-65970281 MsG0880045884.01.T01:CDS
AACTCTAGCAATTCAGGTAA+CGG 0.581378 8:-65970384 MsG0880045884.01.T01:CDS
GAAAATTGATCTAACACCAT+GGG 0.583475 8:-65970756 MsG0880045884.01.T01:CDS
CACCTAGCGAACTACCACTT+CGG 0.583535 8:+65969730 None:intergenic
CCTCTTTGGATGGTTTCAAG+TGG 0.585328 8:+65970719 None:intergenic
TCCATCATTTGCAACAACCT+CGG 0.586818 8:-65970539 MsG0880045884.01.T01:CDS
ACATAGAAAACATCTTCTTG+TGG 0.596961 8:+65969969 None:intergenic
GGGTGGGAAGAATGAGAGTG+TGG 0.597075 8:+65970631 None:intergenic
GGAAGGAATCGGAGATCCCA+TGG 0.597088 8:+65970740 None:intergenic
AAGCCTGTGGCGTTTCGAAG+TGG 0.599152 8:-65969642 MsG0880045884.01.T01:CDS
ACAAGACGCCCTGCAAAGGG+TGG 0.600540 8:+65970614 None:intergenic
ACACCAACGTTGTTCCGAAG+TGG 0.610004 8:-65969744 MsG0880045884.01.T01:CDS
TGGGAAGAATGAGAGTGTGG+TGG 0.610635 8:+65970634 None:intergenic
TTATTTCGGAAACGCAGTAG+TGG 0.625782 8:-65969898 MsG0880045884.01.T01:CDS
AGAAAATTGATCTAACACCA+TGG 0.626932 8:-65970757 MsG0880045884.01.T01:CDS
CCACTTGAAACCATCCAAAG+AGG 0.628828 8:-65970719 MsG0880045884.01.T01:CDS
CTACCACTTCGGAACAACGT+TGG 0.629293 8:+65969741 None:intergenic
CAAGACGCCCTGCAAAGGGT+GGG 0.638377 8:+65970615 None:intergenic
AAGAATCTTCCATTTCACAA+AGG 0.647468 8:-65970117 MsG0880045884.01.T01:CDS
TGGTGTGAGGATGAAAGCAG+GGG 0.647651 8:-65969871 MsG0880045884.01.T01:CDS
CGAAGTGGTAGTTCGCTAGG+TGG 0.653912 8:-65969729 MsG0880045884.01.T01:CDS
CAGGTAACGGAGTTAAGTGA+CGG 0.655221 8:-65970371 MsG0880045884.01.T01:CDS
AGCACAAAACACAACTGTCG+CGG 0.659280 8:-65970495 MsG0880045884.01.T01:CDS
TGTGAGGATGAAAGCAGGGG+AGG 0.662636 8:-65969868 MsG0880045884.01.T01:CDS
CAGTAGTGGTTAATGGTGTG+AGG 0.690545 8:-65969884 MsG0880045884.01.T01:CDS
TTTCACAAAGGAGAAAATCG+CGG 0.713213 8:-65970105 MsG0880045884.01.T01:CDS
ACTTGGCGTCTACAACAGCG+TGG 0.778771 8:+65970324 None:intergenic
TGATGATAGATGTTAGACCA+AGG 0.780037 8:-65969944 MsG0880045884.01.T01:CDS
CGTGGACGAAGAGTGCACCG+AGG 0.794757 8:+65970522 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTAGATTTATCTGTTT+CGG + Chr8:65970287-65970306 None:intergenic 15.0%
!! AGATTATTATTGTTGTCAAT+TGG + Chr8:65969545-65969564 None:intergenic 20.0%
! ATTGGAATTTCAATATCGTT+TGG + Chr8:65970127-65970146 None:intergenic 25.0%
! TACATTCTGATGAGAAAATA+AGG - Chr8:65970548-65970567 MsG0880045884.01.T01:CDS 25.0%
! TTGGAATTTCAATATCGTTT+GGG + Chr8:65970126-65970145 None:intergenic 25.0%
!!! CACTCATTTTTTACACTTAA+CGG - Chr8:65969902-65969921 MsG0880045884.01.T01:CDS 25.0%
AAATAATCTTCTTCCAAAGG+TGG + Chr8:65970427-65970446 None:intergenic 30.0%
AAGAATCTTCCATTTCACAA+AGG - Chr8:65970222-65970241 MsG0880045884.01.T01:CDS 30.0%
AAGCACTATTATCTCATGTT+TGG - Chr8:65970317-65970336 MsG0880045884.01.T01:CDS 30.0%
ACATAGAAAACATCTTCTTG+TGG + Chr8:65970373-65970392 None:intergenic 30.0%
AGAAAATTGATCTAACACCA+TGG - Chr8:65969582-65969601 MsG0880045884.01.T01:CDS 30.0%
CGTTAAGTGTAAAAAATGAG+TGG + Chr8:65969904-65969923 None:intergenic 30.0%
GAAAATTGATCTAACACCAT+GGG - Chr8:65969583-65969602 MsG0880045884.01.T01:CDS 30.0%
GAAGATTCTTTCAGCTAAAT+TGG + Chr8:65970212-65970231 None:intergenic 30.0%
GGGAGTGGTAATAAAAATAA+TGG - Chr8:65970718-65970737 MsG0880045884.01.T01:CDS 30.0%
GTAAAAAATGAGTGGACAAT+TGG + Chr8:65969896-65969915 None:intergenic 30.0%
GTGATTCTATTTGTGTGAAA+GGG + Chr8:65970156-65970175 None:intergenic 30.0%
TGTGATTCTATTTGTGTGAA+AGG + Chr8:65970157-65970176 None:intergenic 30.0%
TTGCCAAAAACATCAAATCT+CGG + Chr8:65970661-65970680 None:intergenic 30.0%
TTTGGCATTTCGTTAATTCT+TGG - Chr8:65970041-65970060 MsG0880045884.01.T01:CDS 30.0%
! CCTTTGGAAGAAGATTATTT+CGG - Chr8:65970427-65970446 MsG0880045884.01.T01:CDS 30.0%
! GTGTCTTTCTTATGAGATTT+TGG - Chr8:65970792-65970811 MsG0880045884.01.T01:CDS 30.0%
! TCTTTCTTATGAGATTTTGG+AGG - Chr8:65970795-65970814 MsG0880045884.01.T01:CDS 30.0%
! TTTGTTTGAGATGGTTGATT+TGG + Chr8:65969679-65969698 None:intergenic 30.0%
!! AAGGGAGTTTTGGTATTTTA+TGG + Chr8:65970087-65970106 None:intergenic 30.0%
!! AGGGAGTTTTGGTATTTTAT+GGG + Chr8:65970086-65970105 None:intergenic 30.0%
!! TTTTTGGCAATGATTTTGGT+TGG - Chr8:65970671-65970690 MsG0880045884.01.T01:CDS 30.0%
!!! ATTTGGTTTTGGTTAGGAAT+GGG + Chr8:65969662-65969681 None:intergenic 30.0%
!!! GATGTTTTTGGCAATGATTT+TGG - Chr8:65970667-65970686 MsG0880045884.01.T01:CDS 30.0%
!!! TTTTGGCAATGATTTTGGTT+GGG - Chr8:65970672-65970691 MsG0880045884.01.T01:CDS 30.0%
AAAAATGAGTGGACAATTGG+AGG + Chr8:65969893-65969912 None:intergenic 35.0%
AAAACCAACTCTAGCAATTC+AGG - Chr8:65969949-65969968 MsG0880045884.01.T01:CDS 35.0%
AACTCTAGCAATTCAGGTAA+CGG - Chr8:65969955-65969974 MsG0880045884.01.T01:CDS 35.0%
CCGAAATAATCTTCTTCCAA+AGG + Chr8:65970430-65970449 None:intergenic 35.0%
GGAAAAATTAGTGTGTTTGC+TGG - Chr8:65970739-65970758 MsG0880045884.01.T01:CDS 35.0%
GGATTCTAAGCTTTCTTCAA+AGG + Chr8:65970191-65970210 None:intergenic 35.0%
TAAGGAACCATTACGAATCT+TGG - Chr8:65970566-65970585 MsG0880045884.01.T01:CDS 35.0%
TGATGATAGATGTTAGACCA+AGG - Chr8:65970395-65970414 MsG0880045884.01.T01:CDS 35.0%
TTCTCAACCAAGATTCGTAA+TGG + Chr8:65970576-65970595 None:intergenic 35.0%
TTTCACAAAGGAGAAAATCG+CGG - Chr8:65970234-65970253 MsG0880045884.01.T01:CDS 35.0%
! CGATTTTCTCCTTTGTGAAA+TGG + Chr8:65970234-65970253 None:intergenic 35.0%
! TCTCCGAGATTTGATGTTTT+TGG - Chr8:65970655-65970674 MsG0880045884.01.T01:CDS 35.0%
!! GTTGTTGTTGTTGTTGTTTG+TGG + Chr8:65969771-65969790 None:intergenic 35.0%
!! TATGAGATTTTGGAGGCTTT+GGG - Chr8:65970802-65970821 MsG0880045884.01.T01:CDS 35.0%
!! TGGAAAGTGTTTGTTTGAGA+TGG + Chr8:65969688-65969707 None:intergenic 35.0%
!! TTATGAGATTTTGGAGGCTT+TGG - Chr8:65970801-65970820 MsG0880045884.01.T01:CDS 35.0%
!!! GATTTGGTTTTGGTTAGGAA+TGG + Chr8:65969663-65969682 None:intergenic 35.0%
!!! TGAGATGGTTGATTTGGTTT+TGG + Chr8:65969673-65969692 None:intergenic 35.0%
!!! TGGTTGATTTGGTTTTGGTT+AGG + Chr8:65969668-65969687 None:intergenic 35.0%
!!! TTTGGCAATGATTTTGGTTG+GGG - Chr8:65970673-65970692 MsG0880045884.01.T01:CDS 35.0%
AACGAAATGCCAAAACGACT+TGG + Chr8:65970035-65970054 None:intergenic 40.0%
AACTCCCTTCATTTCAACGT+TGG - Chr8:65970098-65970117 MsG0880045884.01.T01:CDS 40.0%
AGCTTTCTTCAAAGGAGTCT+TGG + Chr8:65970183-65970202 None:intergenic 40.0%
ATGGGAACCTTTCGAGATTT+GGG + Chr8:65970068-65970087 None:intergenic 40.0%
GGAACCAACGTTGAAATGAA+GGG + Chr8:65970105-65970124 None:intergenic 40.0%
GGGAAATGATGTTGAGTTCT+TGG - Chr8:65970822-65970841 MsG0880045884.01.T01:CDS 40.0%
GTTACCTGAATTGCTAGAGT+TGG + Chr8:65969956-65969975 None:intergenic 40.0%
TATGGGAACCTTTCGAGATT+TGG + Chr8:65970069-65970088 None:intergenic 40.0%
TCCATCATTTGCAACAACCT+CGG - Chr8:65969800-65969819 MsG0880045884.01.T01:CDS 40.0%
TGTTGTGGATATCACCTTGA+TGG + Chr8:65969510-65969529 None:intergenic 40.0%
TTAAGTGACGGCATCTTCAT+TGG - Chr8:65969980-65969999 MsG0880045884.01.T01:CDS 40.0%
TTATTTCGGAAACGCAGTAG+TGG - Chr8:65970441-65970460 MsG0880045884.01.T01:CDS 40.0%
TTCGCTAGGTGGTAATATGT+TGG - Chr8:65970621-65970640 MsG0880045884.01.T01:CDS 40.0%
! CGTTGAAATGAAGGGAGTTT+TGG + Chr8:65970097-65970116 None:intergenic 40.0%
! TGGTGCTTGAATTGTTGTTG+TGG + Chr8:65969525-65969544 None:intergenic 40.0%
! TGGTTTCAAGTGGAAGGAAT+CGG + Chr8:65969613-65969632 None:intergenic 40.0%
! TTTGGATGGTTTCAAGTGGA+AGG + Chr8:65969619-65969638 None:intergenic 40.0%
!! GAGGTTCTAGAGTGTGAAAT+CGG - Chr8:65970490-65970509 MsG0880045884.01.T01:CDS 40.0%
AATGGTGTGAGGATGAAAGC+AGG - Chr8:65970466-65970485 MsG0880045884.01.T01:CDS 45.0%
ACCAAGGATGATTCCACCTT+TGG - Chr8:65970411-65970430 MsG0880045884.01.T01:CDS 45.0%
ACCGAGGTTGTTGCAAATGA+TGG + Chr8:65969804-65969823 None:intergenic 45.0%
AGCACAAAACACAACTGTCG+CGG - Chr8:65969844-65969863 MsG0880045884.01.T01:CDS 45.0%
ATGGTGTGAGGATGAAAGCA+GGG - Chr8:65970467-65970486 MsG0880045884.01.T01:CDS 45.0%
ATTACAAGACGCCCTGCAAA+GGG + Chr8:65969731-65969750 None:intergenic 45.0%
CAGTAGTGGTTAATGGTGTG+AGG - Chr8:65970455-65970474 MsG0880045884.01.T01:CDS 45.0%
CCACTTGAAACCATCCAAAG+AGG - Chr8:65969620-65969639 MsG0880045884.01.T01:CDS 45.0%
CCTCTTTGGATGGTTTCAAG+TGG + Chr8:65969623-65969642 None:intergenic 45.0%
GAAAAGAAGGCCTCTTTGGA+TGG + Chr8:65969633-65969652 None:intergenic 45.0%
GAGGTGTGTAGTTGGATTGA+AGG + Chr8:65969874-65969893 None:intergenic 45.0%
GGAAACGCAGTAGTGGTTAA+TGG - Chr8:65970448-65970467 MsG0880045884.01.T01:CDS 45.0%
GGGAACCAACGTTGAAATGA+AGG + Chr8:65970106-65970125 None:intergenic 45.0%
TCCAAAGGTGGAATCATCCT+TGG + Chr8:65970415-65970434 None:intergenic 45.0%
TCTTGGTCCCAAATCTCGAA+AGG - Chr8:65970058-65970077 MsG0880045884.01.T01:CDS 45.0%
! GACAATTGGAGGTGTGTAGT+TGG + Chr8:65969882-65969901 None:intergenic 45.0%
! GACAGTTGTGTTTTGTGCTG+TGG + Chr8:65969843-65969862 None:intergenic 45.0%
! TGTGTGAAAGGGAAGCGTAT+TGG + Chr8:65970145-65970164 None:intergenic 45.0%
! TTGTAGACGCCAAGTCGTTT+TGG - Chr8:65970023-65970042 MsG0880045884.01.T01:CDS 45.0%
!! CAGGTAACGGAGTTAAGTGA+CGG - Chr8:65969968-65969987 MsG0880045884.01.T01:CDS 45.0%
!! GAATGGGTTGGTGGAAAAGA+AGG + Chr8:65969646-65969665 None:intergenic 45.0%
!! GTTTGTGGTGTCGTTGTGTT+GGG + Chr8:65969756-65969775 None:intergenic 45.0%
!! TGTTTGTGGTGTCGTTGTGT+TGG + Chr8:65969757-65969776 None:intergenic 45.0%
!! TTTGGTTAGGAATGGGTTGG+TGG + Chr8:65969655-65969674 None:intergenic 45.0%
!!! GGTTTTGGTTAGGAATGGGT+TGG + Chr8:65969658-65969677 None:intergenic 45.0%
ACACCAACGTTGTTCCGAAG+TGG - Chr8:65970595-65970614 MsG0880045884.01.T01:CDS 50.0%
CACCTAGCGAACTACCACTT+CGG + Chr8:65970612-65970631 None:intergenic 50.0%
CATTCTTCCCACCCTTTGCA+GGG - Chr8:65969717-65969736 MsG0880045884.01.T01:CDS 50.0%
CTACCACTTCGGAACAACGT+TGG + Chr8:65970601-65970620 None:intergenic 50.0%
GATTACAAGACGCCCTGCAA+AGG + Chr8:65969732-65969751 None:intergenic 50.0%
GGTGGAAAAGAAGGCCTCTT+TGG + Chr8:65969637-65969656 None:intergenic 50.0%
TCATTCTTCCCACCCTTTGC+AGG - Chr8:65969716-65969735 MsG0880045884.01.T01:CDS 50.0%
TGGTGTGAGGATGAAAGCAG+GGG - Chr8:65970468-65970487 MsG0880045884.01.T01:CDS 50.0%
TTCCGAAGTGGTAGTTCGCT+AGG - Chr8:65970607-65970626 MsG0880045884.01.T01:CDS 50.0%
! GTGTTTGCTGGTGCTGAAGA+AGG - Chr8:65970751-65970770 MsG0880045884.01.T01:CDS 50.0%
! TGGGAAGAATGAGAGTGTGG+TGG + Chr8:65969708-65969727 None:intergenic 50.0%
! TTGTGTTTTGTGCTGTGGCG+TGG + Chr8:65969838-65969857 None:intergenic 50.0%
!! TTTTGGTTGGGGAAAGCCTG+TGG - Chr8:65970684-65970703 MsG0880045884.01.T01:CDS 50.0%
AAGCCTGTGGCGTTTCGAAG+TGG - Chr8:65970697-65970716 MsG0880045884.01.T01:CDS 55.0%
ACTTGGCGTCTACAACAGCG+TGG + Chr8:65970018-65970037 None:intergenic 55.0%
AGCCTGTGGCGTTTCGAAGT+GGG - Chr8:65970698-65970717 MsG0880045884.01.T01:CDS 55.0%
CGAAGTGGTAGTTCGCTAGG+TGG - Chr8:65970610-65970629 MsG0880045884.01.T01:CDS 55.0%
GGAAGGAATCGGAGATCCCA+TGG + Chr8:65969602-65969621 None:intergenic 55.0%
GGGTGGGAAGAATGAGAGTG+TGG + Chr8:65969711-65969730 None:intergenic 55.0%
TGTGAGGATGAAAGCAGGGG+AGG - Chr8:65970471-65970490 MsG0880045884.01.T01:CDS 55.0%
ACAAGACGCCCTGCAAAGGG+TGG + Chr8:65969728-65969747 None:intergenic 60.0%
CAAGACGCCCTGCAAAGGGT+GGG + Chr8:65969727-65969746 None:intergenic 60.0%
CTCCCACTTCGAAACGCCAC+AGG + Chr8:65970703-65970722 None:intergenic 60.0%
GTGGCGTTTCGAAGTGGGAG+TGG - Chr8:65970703-65970722 MsG0880045884.01.T01:CDS 60.0%
! CGTGGACGAAGAGTGCACCG+AGG + Chr8:65969820-65969839 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 65969500 65970861 65969500 ID=MsG0880045884.01;Name=MsG0880045884.01
Chr8 mRNA 65969500 65970861 65969500 ID=MsG0880045884.01.T01;Parent=MsG0880045884.01;Name=MsG0880045884.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|453
Chr8 exon 65969500 65970861 65969500 ID=MsG0880045884.01.T01:exon:13709;Parent=MsG0880045884.01.T01
Chr8 CDS 65969500 65970861 65969500 ID=MsG0880045884.01.T01:cds;Parent=MsG0880045884.01.T01
Gene Sequence

>MsG0880045884.01.T01

ATGGATTCCATCAAGGTGATATCCACAACAACAATTCAAGCACCAATTGACAACAATAATAATCTCAATTCAACTGATTATCAGAAAATTGATCTAACACCATGGGATCTCCGATTCCTTCCACTTGAAACCATCCAAAGAGGCCTTCTTTTCCACCAACCCATTCCTAACCAAAACCAAATCAACCATCTCAAACAAACACTTTCCACCACACTCTCATTCTTCCCACCCTTTGCAGGGCGTCTTGTAATCACCCAACACAACGACACCACAAACAACAACAACAACAACACATCATGTTCCATCATTTGCAACAACCTCGGTGCACTCTTCGTCCACGCCACAGCACAAAACACAACTGTCGCGGACATCCTTCAATCCAACTACACACCTCCAATTGTCCACTCATTTTTTACACTTAACGGTGTTAAAAACTACGAAGCTACGTCAAAACCAACTCTAGCAATTCAGGTAACGGAGTTAAGTGACGGCATCTTCATTGGCTTCACATGCAACCACGCTGTTGTAGACGCCAAGTCGTTTTGGCATTTCGTTAATTCTTGGTCCCAAATCTCGAAAGGTTCCCATAAAATACCAAAACTCCCTTCATTTCAACGTTGGTTCCCAAACGATATTGAAATTCCAATACGCTTCCCTTTCACACAAATAGAATCACAAAACCAAGACTCCTTTGAAGAAAGCTTAGAATCCAATTTAGCTGAAAGAATCTTCCATTTCACAAAGGAGAAAATCGCGGAACTGAAATCAAAAGCAAACGCAGAAGCCGAAACAGATAAATCTAAAATATCTTCGCTTCAAGCACTATTATCTCATGTTTGGCGTTCTGTTGTTCGTTGCAAAAAACACGATCCACAAGAAGATGTTTTCTATGTGTTGATGATAGATGTTAGACCAAGGATGATTCCACCTTTGGAAGAAGATTATTTCGGAAACGCAGTAGTGGTTAATGGTGTGAGGATGAAAGCAGGGGAGGTTCTAGAGTGTGAAATCGGAAAAGTTGCTTTAGAGATAAACAAGATGATTTCGTTACATTCTGATGAGAAAATAAGGAACCATTACGAATCTTGGTTGAGAACACCAACGTTGTTCCGAAGTGGTAGTTCGCTAGGTGGTAATATGTTGGCAATGAGCAGTTCTCCGAGATTTGATGTTTTTGGCAATGATTTTGGTTGGGGAAAGCCTGTGGCGTTTCGAAGTGGGAGTGGTAATAAAAATAATGGAAAAATTAGTGTGTTTGCTGGTGCTGAAGAAGGTAGTATAGATGTTGAAATGTGTCTTTCTTATGAGATTTTGGAGGCTTTGGGAAATGATGTTGAGTTCTTGGTTCCTAGTTCTAAGTAA
Protein sequence

>MsG0880045884.01.T01

MDSIKVISTTTIQAPIDNNNNLNSTDYQKIDLTPWDLRFLPLETIQRGLLFHQPIPNQNQINHLKQTLSTTLSFFPPFAGRLVITQHNDTTNNNNNNTSCSIICNNLGALFVHATAQNTTVADILQSNYTPPIVHSFFTLNGVKNYEATSKPTLAIQVTELSDGIFIGFTCNHAVVDAKSFWHFVNSWSQISKGSHKIPKLPSFQRWFPNDIEIPIRFPFTQIESQNQDSFEESLESNLAERIFHFTKEKIAELKSKANAEAETDKSKISSLQALLSHVWRSVVRCKKHDPQEDVFYVLMIDVRPRMIPPLEEDYFGNAVVVNGVRMKAGEVLECEIGKVALEINKMISLHSDEKIRNHYESWLRTPTLFRSGSSLGGNMLAMSSSPRFDVFGNDFGWGKPVAFRSGSGNKNNGKISVFAGAEEGSIDVEMCLSYEILEALGNDVEFLVPSSK*