AlfalfaGEDB | MsG0380016143.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016143.01

KEGG: -
GO: -
PFAM: PF05498

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016143.01.T01	XP_003601688.1	91.398	93	5	2	2	93	45	135	6.51E-54	174

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016143.01.T01	Q2HIM9	60	75	28	1	19	93	41	113	4.02E-25	93.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016143.01.T01	G7JAS2	91.398	93	5	2	2	93	45	135	3.11e-54	174

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001588.01	MsG0380016143.01	0.801189	1.012532e-48	4.805257e-46
MsG0180003905.01	MsG0380016143.01	0.812723	3.673086e-51	2.353076e-48
MsG0180005367.01	MsG0380016143.01	0.805825	1.107102e-49	5.915850e-47
MsG0180006076.01	MsG0380016143.01	-0.802817	4.686080e-49	2.317879e-46
MsG0180006182.01	MsG0380016143.01	0.825810	3.827025e-54	3.525583e-51
MsG0380012818.01	MsG0380016143.01	0.819037	1.433266e-52	1.090810e-49
MsG0380015656.01	MsG0380016143.01	0.827837	1.254473e-54	1.225170e-51
MsG0380016143.01	MsG0480018111.01	0.805251	1.460601e-49	7.690092e-47
MsG0380016143.01	MsG0480018228.01	0.804581	2.016674e-49	1.043643e-46
MsG0380016143.01	MsG0480018767.01	0.823531	1.317257e-53	1.137174e-50
MsG0380016143.01	MsG0480023064.01	0.805565	1.255438e-49	6.663489e-47
MsG0380016143.01	MsG0480023375.01	-0.807990	3.859351e-50	2.180784e-47
MsG0380016143.01	MsG0480023626.01	0.810309	1.229076e-50	7.383280e-48
MsG0380016143.01	MsG0480023663.01	0.828864	7.090547e-55	7.134968e-52
MsG0380016143.01	MsG0580024773.01	0.816524	5.289468e-52	3.756815e-49
MsG0380016143.01	MsG0580025153.01	-0.804244	2.370970e-49	1.216325e-46
MsG0380016143.01	MsG0580025464.01	0.809952	1.466823e-50	8.730244e-48
MsG0380016143.01	MsG0580028330.01	0.805648	1.206000e-49	6.415002e-47
MsG0380016143.01	MsG0580028364.01	0.811373	7.232085e-51	4.469041e-48
MsG0380016143.01	MsG0780038393.01	0.819838	9.412126e-53	7.324422e-50
MsG0380016143.01	MsG0780040276.01	0.834011	3.835997e-56	4.494197e-53
MsG0380016143.01	MsG0780040978.01	0.824046	9.980130e-54	8.741432e-51
MsG0380016143.01	MsG0780040990.01	0.807869	4.093272e-50	2.305577e-47
MsG0380016143.01	MsG0880044139.01	0.806833	6.789706e-50	3.722806e-47
MsG0380016143.01	MsG0880044356.01	0.825549	4.412329e-54	4.034408e-51
MsG0380016143.01	MsG0880045584.01	0.812518	4.071742e-51	2.594422e-48
MsG0380016143.01	MsG0880045963.01	0.800568	1.355828e-48	6.333909e-46
MsG0380016143.01	MsG0880045989.01	0.807562	4.757284e-50	2.658190e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016143.01.T01	MTR_3g084350	91.398	93	5	2	2	93	45	135	7.88e-58	174
MsG0380016143.01.T01	MTR_7g108600	53.750	80	35	2	4	82	37	115	4.14e-22	83.2
MsG0380016143.01.T01	MTR_4g119860	54.321	81	29	3	11	84	47	126	9.09e-22	82.8
MsG0380016143.01.T01	MTR_4g088685	62.121	66	23	2	21	84	44	109	1.84e-21	81.6
MsG0380016143.01.T01	MTR_7g113080	54.545	77	30	3	10	82	50	125	1.63e-20	79.7
MsG0380016143.01.T01	MTR_6g060260	61.538	65	18	2	21	84	45	103	5.38e-20	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016143.01.T01	AT4G13950	60.000	75	28	1	19	93	41	113	4.10e-26	93.6
MsG0380016143.01.T01	AT4G15800	58.140	86	31	3	3	84	32	116	1.82e-24	89.4
MsG0380016143.01.T01	AT3G23805	68.421	57	18	0	28	84	55	111	1.45e-23	87.0
MsG0380016143.01.T01	AT1G02900	60.274	73	27	2	11	82	47	118	7.91e-23	85.1
MsG0380016143.01.T01	AT1G28270	58.824	68	27	1	21	87	43	110	6.59e-21	80.1
MsG0380016143.01.T01	AT2G33775	58.462	65	26	1	21	84	43	107	8.75e-21	79.7
MsG0380016143.01.T01	AT3G05490	65.000	60	19	2	26	84	61	119	2.24e-20	79.0
MsG0380016143.01.T01	AT3G16570	64.407	59	19	2	27	84	80	137	1.38e-19	77.4
MsG0380016143.01.T01	AT3G16570	64.407	59	19	2	27	84	80	137	1.38e-19	77.4
MsG0380016143.01.T01	AT3G16570	64.407	59	19	2	27	84	80	137	1.38e-19	77.4

Find 18 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAATCCATCAACATTTC+AGG	0.211686	3:+79567153	None:intergenic
AGAGAGTAATAGAAGAGTTT+TGG	0.275974	3:-79567133	MsG0380016143.01.T01:CDS
TCTTCTATGACAATTATAAT+AGG	0.319384	3:+79567038	None:intergenic
CAAACACTCTCCAATGGTTT+TGG	0.332682	3:+79567182	None:intergenic
ACCTCACAACCTCTACTATA+AGG	0.344011	3:+79567006	None:intergenic
TTCTTGACTTTGAGCACCTC+TGG	0.366948	3:+79566969	None:intergenic
GACAGAACCTGAAATGTTGA+TGG	0.394954	3:-79567160	MsG0380016143.01.T01:CDS
AGGGTTTGTACCAAAACCAT+TGG	0.510060	3:-79567192	MsG0380016143.01.T01:CDS
TGATACATTGAAGAGAGATA+TGG	0.513283	3:-79567085	MsG0380016143.01.T01:CDS
TTCTGTCAAACACTCTCCAA+TGG	0.555363	3:+79567176	None:intergenic
TCCTTATAGTAGAGGTTGTG+AGG	0.577106	3:-79567007	MsG0380016143.01.T01:CDS
CAAGCTAATCCTTATAGTAG+AGG	0.579620	3:-79567015	MsG0380016143.01.T01:CDS
CAATTATAATAGGAAGCACC+AGG	0.603685	3:+79567048	None:intergenic
TGATGCAGTAATCACAAAGA+GGG	0.632909	3:-79567211	MsG0380016143.01.T01:CDS
GTTATTACTGCATGTGCCAG+AGG	0.645620	3:-79566985	MsG0380016143.01.T01:CDS
ATGATGCAGTAATCACAAAG+AGG	0.663336	3:-79567212	MsG0380016143.01.T01:CDS
ATGGTTCCTTGTGATAGACC+TGG	0.696540	3:-79567066	MsG0380016143.01.T01:CDS
GAAGCACCAGGTCTATCACA+AGG	0.718482	3:+79567060	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCTTCTATGACAATTATAAT+AGG	+	Chr3:79567138-79567157	None:intergenic	20.0%
	TGATACATTGAAGAGAGATA+TGG	-	Chr3:79567088-79567107	MsG0380016143.01.T01:CDS	30.0%
!	AGAGAGTAATAGAAGAGTTT+TGG	-	Chr3:79567040-79567059	MsG0380016143.01.T01:CDS	30.0%
	ATGATGCAGTAATCACAAAG+AGG	-	Chr3:79566961-79566980	MsG0380016143.01.T01:CDS	35.0%
	CAAGCTAATCCTTATAGTAG+AGG	-	Chr3:79567158-79567177	MsG0380016143.01.T01:CDS	35.0%
	TCTGAATCCATCAACATTTC+AGG	+	Chr3:79567023-79567042	None:intergenic	35.0%
	TGATGCAGTAATCACAAAGA+GGG	-	Chr3:79566962-79566981	MsG0380016143.01.T01:CDS	35.0%
!!	CAATTATAATAGGAAGCACC+AGG	+	Chr3:79567128-79567147	None:intergenic	35.0%
	ACCTCACAACCTCTACTATA+AGG	+	Chr3:79567170-79567189	None:intergenic	40.0%
	AGGGTTTGTACCAAAACCAT+TGG	-	Chr3:79566981-79567000	MsG0380016143.01.T01:CDS	40.0%
	GACAGAACCTGAAATGTTGA+TGG	-	Chr3:79567013-79567032	MsG0380016143.01.T01:CDS	40.0%
	TCCTTATAGTAGAGGTTGTG+AGG	-	Chr3:79567166-79567185	MsG0380016143.01.T01:CDS	40.0%
	TTCTGTCAAACACTCTCCAA+TGG	+	Chr3:79567000-79567019	None:intergenic	40.0%
!	CAAACACTCTCCAATGGTTT+TGG	+	Chr3:79566994-79567013	None:intergenic	40.0%
	ATGGTTCCTTGTGATAGACC+TGG	-	Chr3:79567107-79567126	MsG0380016143.01.T01:CDS	45.0%
	GTTATTACTGCATGTGCCAG+AGG	-	Chr3:79567188-79567207	MsG0380016143.01.T01:CDS	45.0%
	TTCTTGACTTTGAGCACCTC+TGG	+	Chr3:79567207-79567226	None:intergenic	45.0%
!	GAAGCACCAGGTCTATCACA+AGG	+	Chr3:79567116-79567135	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	79566957	79567238	79566957	ID=MsG0380016143.01;Name=MsG0380016143.01
Chr3	mRNA	79566957	79567238	79566957	ID=MsG0380016143.01.T01;Parent=MsG0380016143.01;Name=MsG0380016143.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|93
Chr3	exon	79566957	79567238	79566957	ID=MsG0380016143.01.T01:exon:15217;Parent=MsG0380016143.01.T01
Chr3	CDS	79566957	79567238	79566957	ID=MsG0380016143.01.T01:cds;Parent=MsG0380016143.01.T01

Gene Sequence	>MsG0380016143.01.T01 ATGGATGATGCAGTAATCACAAAGAGGGTTTGTACCAAAACCATTGGAGAGTGTTTGACAGAACCTGAAATGTTGATGGATTCAGAGAGTAATAGAAGAGTTTTGGCAATGCAGAAAAAGTACATAAGCTATGATACATTGAAGAGAGATATGGTTCCTTGTGATAGACCTGGTGCTTCCTATTATAATTGTCATAGAAGACAAGCTAATCCTTATAGTAGAGGTTGTGAGGTTATTACTGCATGTGCCAGAGGTGCTCAAAGTCAAGAAATCAACACTTGA
Protein sequence	>MsG0380016143.01.T01 MDDAVITKRVCTKTIGECLTEPEMLMDSESNRRVLAMQKKYISYDTLKRDMVPCDRPGASYYNCHRRQANPYSRGCEVITACARGAQSQEINT*