AlfalfaGEDB | MsG0180005367.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005367.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005367.01.T01	PHT50556.1	74.254	268	46	1	1	245	1	268	2.94E-139	402
MsG0180005367.01.T02	PHT50556.1	74.254	268	46	1	1	245	1	268	2.94E-139	402

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005367.01.T01	Q9SZ96	72.775	191	44	3	1	183	1	191	9.57E-90	271
MsG0180005367.01.T02	Q9SZ96	72.775	191	44	3	1	183	1	191	9.57E-90	271

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005367.01.T01	A0A2N9J1Q6	80.597	268	29	1	1	245	1	268	6.34e-153	436
MsG0180005367.01.T02	A0A2N9J1Q6	80.597	268	29	1	1	245	1	268	6.34e-153	436

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049008.01	MsG0180005367.01	0.812444	4.226329e-51	2.687795e-48
MsG0180000535.01	MsG0180005367.01	0.830779	2.422661e-55	2.578238e-52
MsG0180003905.01	MsG0180005367.01	0.852904	3.398272e-61	7.210775e-58
MsG0180004705.01	MsG0180005367.01	0.807705	4.436122e-50	2.488052e-47
MsG0180004828.01	MsG0180005367.01	0.806784	6.951889e-50	3.806867e-47
MsG0180005325.01	MsG0180005367.01	0.809507	1.828596e-50	1.075587e-47
MsG0180005367.01	MsG0180005655.01	0.821987	3.013815e-53	2.490240e-50
MsG0180005367.01	MsG0180005741.01	0.854912	8.973211e-62	2.035608e-58
MsG0180005367.01	MsG0180005898.01	-0.815199	1.044971e-51	7.156986e-49
MsG0180005367.01	MsG0180006076.01	-0.818735	1.678351e-52	1.266626e-49

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005367.01.T01	MTR_1g098240	82.723	191	25	2	1	183	1	191	5.30e-104	304
MsG0180005367.01.T01	MTR_1g098240	95.918	98	2	1	148	245	225	320	4.07e-60	192
MsG0180005367.01.T01	MTR_3g095130	64.921	191	59	3	1	183	1	191	2.95e-79	242
MsG0180005367.01.T01	MTR_3g095130	83.505	97	16	0	148	244	225	321	7.46e-53	174
MsG0180005367.01.T01	MTR_8g092190	61.538	195	67	3	1	187	1	195	5.97e-77	236
MsG0180005367.01.T01	MTR_8g092190	75.510	98	21	1	150	244	227	324	4.61e-46	157
MsG0180005367.01.T01	MTR_3g095130	83.505	97	16	0	148	244	176	272	2.48e-53	174
MsG0180005367.01.T01	MTR_3g095130	61.972	142	46	3	50	183	1	142	1.70e-50	166
MsG0180005367.01.T01	MTR_1g026590	49.730	185	81	4	17	190	15	198	1.81e-36	132
MsG0180005367.01.T01	MTR_1g026590	48.958	96	40	3	150	242	227	316	2.46e-20	88.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005367.01.T01	AT4G09810	72.775	191	44	3	1	183	1	191	8.08e-93	273
MsG0180005367.01.T01	AT4G09810	72.775	191	44	3	1	183	1	191	9.75e-91	271
MsG0180005367.01.T01	AT4G09810	78.571	98	21	0	146	243	223	320	7.13e-52	172
MsG0180005367.01.T01	AT1G34020	73.298	191	43	3	1	183	1	191	3.44e-81	247
MsG0180005367.01.T01	AT1G34020	83.505	97	16	0	147	243	224	320	1.40e-53	176
MsG0180005367.01.T01	AT1G34020	73.298	191	43	3	1	183	1	191	3.44e-81	247
MsG0180005367.01.T01	AT1G34020	83.505	97	16	0	147	243	224	320	1.40e-53	176
MsG0180005367.01.T01	AT1G34020	73.298	191	43	3	1	183	1	191	3.44e-81	247
MsG0180005367.01.T01	AT1G34020	83.505	97	16	0	147	243	224	320	1.40e-53	176
MsG0180005367.01.T01	AT4G39390	66.842	190	55	3	2	183	3	192	2.08e-73	227

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Find 40 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAGGTGGGGTGTGT+TGG	0.426524	1:+90880512	None:intergenic
AAAAGGAAGCTAACCGTGGA+TGG	0.545489	1:+90880477	None:intergenic
AAAGAAAGGTGGGGTGTGTT+GGG	0.420540	1:+90880513	None:intergenic
AAAGCTTTGATGGGTGGTTA+TGG	0.350738	1:-90879557	MsG0180005367.01.T01:CDS
AACAAAGAAGCCTGGCAAAA+TGG	0.330737	1:+90879776	None:intergenic
AAGAAGCCTGGCAAAATGGA+AGG	0.591306	1:+90879780	None:intergenic
AAGAATGATCTGAGGTTGAA+AGG	0.535238	1:+90880536	None:intergenic
AAGTGGAGGGAATGTTATGG+AGG	0.604217	1:-90879721	MsG0180005367.01.T01:five_prime_UTR
ACTCCTGCATTATTTGAAAG+TGG	0.523920	1:-90879738	MsG0180005367.01.T01:five_prime_UTR
AGATTGTTGTTAATTGTGTT+TGG	0.399409	1:-90880609	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAGTATTCACTCATTATGA+TGG	-	Chr1:90880463-90880482	MsG0180005367.01.T01:intron	30.0%
ACCATCCAGTATAATAACAA+AGG	+	Chr1:90879726-90879745	None:intergenic	30.0%
ACCTAAGTCATATCTTTGTT+CGG	-	Chr1:90879227-90879246	MsG0180005367.01.T01:CDS	30.0%
ACTAACTTAATCCCACAAAT+CGG	-	Chr1:90879163-90879182	MsG0180005367.01.T01:intron	30.0%
AGTTAAAATTCTAAATGGCC+CGG	+	Chr1:90878946-90878965	None:intergenic	30.0%
ATCAGAAACGAATACTAATG+CGG	-	Chr1:90879291-90879310	MsG0180005367.01.T01:CDS	30.0%
ATCTATAGATTTCTGACACA+TGG	+	Chr1:90878970-90878989	None:intergenic	30.0%
ATGTGTCAGAAATCTATAGA+TGG	-	Chr1:90878969-90878988	MsG0180005367.01.T01:intron	30.0%
ATTGAGTGTATCACTTTAGA+TGG	-	Chr1:90877254-90877273	MsG0180005367.01.T01:intron	30.0%
CAGAAATCTATAGATGGAAT+TGG	-	Chr1:90878975-90878994	MsG0180005367.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	90876677	90880626	90876677	ID=MsG0180005367.01;Name=MsG0180005367.01
Chr1	mRNA	90876677	90880626	90876677	ID=MsG0180005367.01.T01;Parent=MsG0180005367.01;Name=MsG0180005367.01.T01;_AED=0.37;_eAED=0.37;_QI=247\|0.75\|0.8\|1\|0.75\|0.6\|5\|171\|245
Chr1	exon	90879546	90879786	90879546	ID=MsG0180005367.01.T01:exon:48108;Parent=MsG0180005367.01.T01
Chr1	exon	90880491	90880626	90880491	ID=MsG0180005367.01.T01:exon:48107;Parent=MsG0180005367.01.T01
Chr1	five_prime_UTR	90880491	90880626	90880491	ID=MsG0180005367.01.T01:five_prime_utr;Parent=MsG0180005367.01.T01
Chr1	five_prime_UTR	90879676	90879786	90879676	ID=MsG0180005367.01.T01:five_prime_utr;Parent=MsG0180005367.01.T01
Chr1	CDS	90879546	90879675	90879546	ID=MsG0180005367.01.T01:cds;Parent=MsG0180005367.01.T01
Chr1	CDS	90879226	90879410	90879226	ID=MsG0180005367.01.T01:cds;Parent=MsG0180005367.01.T01
Chr1	CDS	90879012	90879143	90879012	ID=MsG0180005367.01.T01:cds;Parent=MsG0180005367.01.T01
Chr1	CDS	90876848	90877138	90876848	ID=MsG0180005367.01.T01:cds;Parent=MsG0180005367.01.T01
Chr1	three_prime_UTR	90876677	90876847	90876677	ID=MsG0180005367.01.T01:three_prime_utr;Parent=MsG0180005367.01.T01
Chr1	mRNA	90876677	90880037	90876677	ID=MsG0180005367.01.T02;Parent=MsG0180005367.01;Name=MsG0180005367.01.T02;_AED=0.35;_eAED=0.35;_QI=362\|0.66\|0.75\|1\|1\|1\|4\|171\|245
Chr1	exon	90879546	90880037	90879546	ID=MsG0180005367.01.T02:exon:48112;Parent=MsG0180005367.01.T02
Chr1	five_prime_UTR	90879676	90880037	90879676	ID=MsG0180005367.01.T02:five_prime_utr;Parent=MsG0180005367.01.T02
Chr1	CDS	90879546	90879675	90879546	ID=MsG0180005367.01.T02:cds;Parent=MsG0180005367.01.T02
Chr1	CDS	90879226	90879410	90879226	ID=MsG0180005367.01.T02:cds;Parent=MsG0180005367.01.T02
Chr1	CDS	90879012	90879143	90879012	ID=MsG0180005367.01.T02:cds;Parent=MsG0180005367.01.T02
Chr1	CDS	90876848	90877138	90876848	ID=MsG0180005367.01.T02:cds;Parent=MsG0180005367.01.T02
Chr1	three_prime_UTR	90876677	90876847	90876677	ID=MsG0180005367.01.T02:three_prime_utr;Parent=MsG0180005367.01.T02

Gene Sequence

>MsG0180005367.01.T01

ATGGCTCCTTCAAGCAAAGCTGAGAAGAAGGCAGCCGTAGATGCTGCGGCATGGATGTTCAATGTTGTCACATCTGTTGGAATTATCATTGTAAACAAAGCTTTGATGGGTGGTTATGGCTTCAGTTTTGCTACAACGTTAACGGGTCTTCATTTTGTTACTACAACTATCATGACGCTCGTACTACGGGTGCTGGGATACGTCCAGCCATCTCATCTTCCATTGTCCGAACTTCTAAAATTTGTTTTCTTTGCTAACTTCTCTATTGTTGGAATGAATGTCAGTTTAATGTGGAACTCAGTTGGATTCTATCAAATTGCCAAATTGAGTATGATCCCTGTATCCTGCTTATTGGAAGTTTGTTTGGACAAGATTCGCTATTCAAGAGACACAAAACTGAGCATAGGTATTGTTCTTTTGGGAGTTGGTGTTTGCACTGTTACTGACATGTTCTTAATCCTGTCATGCACTATTGCAGTTGGGACCAACCTCAGCCAATTTATCTGCATTGGCAGATTTACAGCTGTATCTTTCCAAGTACTTGGACATATGAAGACAATACTTGTTTTAATAATGGGATTCTTTTTCTTTGGGAGGGAAGGTCTTAATCTTCATGTAGTTATAGGGATGATAATAGCTGTTGTTGGAATGATGTGGTATGGTAATGCCTCATCAAAGCCGGGTGGAAAGGAACGTTGGAGTCACTCTCTCCCTACCAGCAAAACAGAAAGTCGGTGA

>MsG0180005367.01.T02

Protein sequence

>MsG0180005367.01.T01

MAPSSKAEKKAAVDAAAWMFNVVTSVGIIIVNKALMGGYGFSFATTLTGLHFVTTTIMTLVLRVLGYVQPSHLPLSELLKFVFFANFSIVGMNVSLMWNSVGFYQIAKLSMIPVSCLLEVCLDKIRYSRDTKLSIGIVLLGVGVCTVTDMFLILSCTIAVGTNLSQFICIGRFTAVSFQVLGHMKTILVLIMGFFFFGREGLNLHVVIGMIIAVVGMMWYGNASSKPGGKERWSHSLPTSKTESR*

>MsG0180005367.01.T02